Re: [Histonet] Crystal/Mount
You're quite right René . Googling "crystalmount" brings up a helpful item (for a competing mounting medium) at the top of the heap, with detailed instructions for use. The third and fifth hits are also helpful. Unfortunately these are followed by hundreds of items, most having nothing to do with microtechnique! The refractive index of CrystalMount is very low (1.35) but it increases with drying to something close to that of a regular resinous mountant (>1.5). See http://www.proscitech.com.au It is unfortunate that the compositions of most resinous mounting media are trade secrets. It's possible (in principle) to make up your own Canada balsam (very expensive) or DPX (might not work; bought DPX is sometimes NBG!). There are plenty of aqueous mountants that can easily be made in any lab or kitchen, some with refractive indices >1.5. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Rene J Buesa rjbu...@yahoo.com > Why you just don't Google it? > René J. > > --- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E] > wrote: > > > From: Yaskovich, Ruth A (NIH/NIDCR) [E] > Subject: [Histonet] Crystal/Mount > > I know this has been asked before. Where can I purchase > Crystal/Mount mounting media? > Thanks > Ruth N.I.H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fire in the lab
Once upon a time in a far away land, we used to boil our embedding molds in boiling soapy water, over an open Bunsen burner, followed by an alcohol rinse then air dry. One time the fire alarm was activated and we had to evacuate the hospital. We were out there quit awhile. When we received the all clear to go back into the hospital, I was the first one back in the lab and the fire department was there, looking into our pot that had boiled out and was smoking up the lab. This wasn't the cause of the first alarm, but it did set off the second. Joe - Original Message - From: "CHRISTIE GOWAN" To: Sent: Friday, February 26, 2010 8:20 AM Subject: [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Crystal/Mount
Thermo Fisher or whatever they are called this week has it Joe - Original Message - From: "Rene J Buesa" To: ; " Ruth A (NIH/NIDCR) [E]Yaskovich" Sent: Friday, February 26, 2010 2:28 PM Subject: Re: [Histonet] Crystal/Mount Why you just don't Google it? René J. --- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote: From: Yaskovich, Ruth A (NIH/NIDCR) [E] Subject: [Histonet] Crystal/Mount To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 26, 2010, 3:10 PM I know this has been asked before. Where can I purchase Crystal/Mount mounting media? Thanks Ruth N.I.H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines
The Leica TP1020 is a good and reliable instrument. René J. --- On Fri, 2/26/10, Parsons, Catherine wrote: From: Parsons, Catherine Subject: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 26, 2010, 3:50 PM I work in a small research lab, and we are shopping for a new tissue embedding machine. Our current unit is a very old AutoTechnicon carousel style system. Space is limited, and we do not have large numbers or constant flow of samples to embed, so we are happy to stay with the carousel style. I would be very interested to hear some reviews of some of the modern models in terms of quality and reliability, as well as ease of use. I have literature on the Leica TP1020 and Thermo Shandon Citadel. Does anyone have others to suggest? Thanks, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] minimum fixation time for needle core prostate biopsies
A poor fixation will affect all the cell components. You may have a result but if the fixation is incomplete, the results will not indicate the real amount. At least 8 hours of fixation will be required. René J. --- On Fri, 2/26/10, Diana McCaig wrote: From: Diana McCaig Subject: [Histonet] minimum fixation time for needle core prostate biopsies To: histonet@lists.utsouthwestern.edu Date: Friday, February 26, 2010, 3:45 PM Is there a minimum time for prostate biopsies to be fixed. Does fixation times affect PIN4's? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines
Thanks for catching my typo..., we are looking for a carousel style tissue processor. Cathy From: Richard Yeo [mailto:r...@wchosp.org] Sent: Friday, February 26, 2010 4:04 PM To: Parsons, Catherine Subject: RE: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines You are talking about processors. Embedding centers are not carousel design. Do you need info on embedding stations or processors? Rich Y From: histonet-boun...@lists.utsouthwestern.edu on behalf of Parsons, Catherine Sent: Fri 2/26/2010 3:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines I work in a small research lab, and we are shopping for a new tissue embedding machine. Our current unit is a very old AutoTechnicon carousel style system. Space is limited, and we do not have large numbers or constant flow of samples to embed, so we are happy to stay with the carousel style. I would be very interested to hear some reviews of some of the modern models in terms of quality and reliability, as well as ease of use. I have literature on the Leica TP1020 and Thermo Shandon Citadel. Does anyone have others to suggest? Thanks, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Replacing an AutoTechnicon - reviews on modern carousel tissue embedding machines
I work in a small research lab, and we are shopping for a new tissue embedding machine. Our current unit is a very old AutoTechnicon carousel style system. Space is limited, and we do not have large numbers or constant flow of samples to embed, so we are happy to stay with the carousel style. I would be very interested to hear some reviews of some of the modern models in terms of quality and reliability, as well as ease of use. I have literature on the Leica TP1020 and Thermo Shandon Citadel. Does anyone have others to suggest? Thanks, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] minimum fixation time for needle core prostate biopsies
Is there a minimum time for prostate biopsies to be fixed. Does fixation times affect PIN4's? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOP for Room Temp./Humidity Monitors
Does anyone have an SOP for monitoring room temp and humidity they can share with me? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Crystal/Mount
Why you just don't Google it? René J. --- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote: From: Yaskovich, Ruth A (NIH/NIDCR) [E] Subject: [Histonet] Crystal/Mount To: "histonet@lists.utsouthwestern.edu" Date: Friday, February 26, 2010, 3:10 PM I know this has been asked before. Where can I purchase Crystal/Mount mounting media? Thanks Ruth N.I.H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Crystal/Mount
I know this has been asked before. Where can I purchase Crystal/Mount mounting media? Thanks Ruth N.I.H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fire in the lab
One of those chemicals could have been chromic acid which will ignite when alcohol is introduced. One of my techs cleaned up the counter with alcohol after measuring chromic acid and we all ran for the extinguisher. She just stood there and shook her hands and stared at the flames. This was right next to all the alcohols, xylenes and stains for the H&E, we did not have automation back then. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephanie Rosenwinkel Sent: Friday, February 26, 2010 12:58 PM To: dkb...@chs.net; christiego...@msn.com Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Fire in the lab A few years back, I also had a fire break out while cleaning off the weighing instrument. There was left over powders of other chemicals on there and my gauze started on fire. So needless to say, please clean up after yourselves! It was Scary, very scary! I tried to yell for help but nothing would come out of my mouth, so I called 911. It was a lesson learned the hard way, for sure. Steph HT(ASCP) > To: christiego...@msn.com > From: dkb...@chs.net > Date: Fri, 26 Feb 2010 09:50:08 -0500 > Subject: Re: [Histonet] Fire in the lab > CC: histonet@lists.utsouthwestern.edu; > histonet-boun...@lists.utsouthwestern.edu > > Not exactly the same, but very similar. We had an automatic stainer by > the sink and one of the techs was washing glassware, the stainer was > running. The water apparently splashed on the wiring and a fire broke > out. We jumped into action. Just as we had been in-service. You are > correct what a mess to clean up! Fire extinquishers are wonderful but > extremely messy. We had totally taken care of the situation by the time > the fire department got here. We actually got accolades for preventing a > much larger fire. It was determined that there was some exposed wires on > the stainer. > A good lesson for all. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkb...@chs.net > > > > > > > > CHRISTIE GOWAN > Sent by: histonet-boun...@lists.utsouthwestern.edu > 02/26/2010 09:21 AM > > To > > cc > > Subject > [Histonet] Fire in the lab > > > > > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into > the processor room and smelled something burning. He noticed our old > paraffin pot had charred looking labels on the outside so he went over, > opened the lid and poof!!! the pot went up in flames. The thermostat had > gone haywire and heated the paraffin to flash point. Opening the lid gave > it the oxygen it needed to ignite. He triggered the alarm, made the > appropriate call and then put it out with an extinguisher. Of course it > kept re-igniting because he could not get behind it to pull the plug. The > fire dept finally was able to get it pulled out and unplugged. Needless to > say the tech was shaken and the room was a mess. I applaud his courage and > am not sure I would have done the same. There was enough xylene and > alcohol on the 4 processors to cause quite an explosion but everything > else was in a flammable cabinet. I was wondering if this type of thing had > ever happened to anyone else?? Needless to say, we have de-comissioned all > old paraffin pots and will order only those with over temp safety > features. I guess I just wanted to remind everyone that fires can happen > in the lab and do probably more often than we hear about. This was the > first time for me and I have been in this business for over 20 years. Take > care and be safe. > > Christie Gowan HT (ASCP) > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __
[Histonet] special coloration for fish gill chloride cells
Hello friends I have some gill tissues for chloride cells localization. Do you know a method for chloride cells coloration except immunohistochemical using NKA antibody??? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, February 26, 2010 9:46 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. NBS/NIST Thermometers (thisis...@aol.com) 2. Control slides for Flag-Tag IHC (Randolph-Habecker, Julie) 3. Control tissue for Fluoro Jade (Van Fleet, Jonilyn (J)) 4. RE: NBS/NIST Thermometers (McMahon, Loralee A) 5. Re: Snap Freezing Tissue (Robert Richmond) 6. voice recognition (Mahoney,Janice A) 7. haematoxylin (Hana Peter) 8. Re: haematoxylin (John Kiernan) 9. Re: shandon's formal fixx and cytoblock kit (shehnaz khan) 10. RE: [ SOLVED ][ Histonet ] Strange circles in IHC slides (Hoekert, W.E.J.) 11. Fire in the lab (CHRISTIE GOWAN) 12. Re: Fire in the lab (dkb...@chs.net) 13. Re: [ SOLVED ][ Histonet ] Strange circles in IHC slides (Alexandra Meinl) 14. Saturday Tech Needed Orange County California (Paula Lucas) 15. ruo antibodies (Vickroy, Jim) 16. RE: Fire in the lab (Stephanie Rosenwinkel) -- Message: 1 Date: Thu, 25 Feb 2010 13:59:23 -0500 From: thisis...@aol.com Subject: [Histonet] NBS/NIST Thermometers To: histonet@lists.utsouthwestern.edu Message-ID: <8cc8463c76e2cbf-55ec-1...@webmail-m063.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Does anyone know how frequently you have to certify your NIST Thermometers (the thermometer you use to calibrate all of the thermometers in the laboratory), including a reference. The certifiicate for my NIST Thermometer does not document the next time it needs to be calibrated. Thank you, Ann Angelo -- Message: 2 Date: Thu, 25 Feb 2010 11:13:38 -0800 From: "Randolph-Habecker, Julie" Subject: [Histonet] Control slides for Flag-Tag IHC To: Message-ID: <040346fa7309bd439c327f97d4c4d69b072c7...@isis.fhcrc.org> Content-Type: text/plain; charset="us-ascii" Folks, I am looking for a control for some Flag-tag IHC I am doing on FFPE tissue. I was wondering if someone could share some slides off of a block of flag transfected cells. THANKS!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabe...@fhcrc.org -- Message: 3 Date: Thu, 25 Feb 2010 15:44:21 -0500 From: "Van Fleet, Jonilyn (J)" Subject: [Histonet] Control tissue for Fluoro Jade To: Message-ID: <11dee9b567c45c42aec620f3b836d53c0325e...@usmdlmdowx027.dow.com> Content-Type: text/plain; charset="us-ascii" Hello Histonet, I am looking for a positive control for the Fluoro Jade Stain consisting of rodent brain tissue demonstrating neuronal necrosis in the hippocampus caused by administration of trimethyltin. Does anyone have access to a stock of controls or can anyone tell me who to contact. I have contacted the AFIP and NSH but neither were able to help. Any information would be greatly appreciated. Thank-you, Jonilyn Van Fleet, HT (ASCP) The Dow Chemical Company 989-636-3539 jvanfl...@dow.com -- Message: 4 Date: Thu, 25 Feb 2010 16:32:35 -0500 From: "McMahon, Loralee A" Subject: RE: [Histonet] NBS/NIST Thermometers To: "thisis...@aol.com" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am in NY State and they just told me yesterday that we need to do it annually. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of thisis...@aol.com [thisis...@aol.com] Sent: Thursday, February 25, 2010 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NBS/NIST Thermometers Does anyone know how frequently you have to certify your NIST Thermometers
RE: [Histonet] Fire in the lab
I did the same thing several years ago - caught my uniform on fire but ran to the sink and all was well in a minute - techs ran to my rescue. But then one of the gang said... "Grab the marshmallows, the supervisor's on fire!"... Gotta love em... Happy Friday!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephanie Rosenwinkel Sent: Friday, February 26, 2010 12:58 To: dkb...@chs.net; christiego...@msn.com Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Fire in the lab A few years back, I also had a fire break out while cleaning off the weighing instrument. There was left over powders of other chemicals on there and my gauze started on fire. So needless to say, please clean up after yourselves! It was Scary, very scary! I tried to yell for help but nothing would come out of my mouth, so I called 911. It was a lesson learned the hard way, for sure. Steph HT(ASCP) > To: christiego...@msn.com > From: dkb...@chs.net > Date: Fri, 26 Feb 2010 09:50:08 -0500 > Subject: Re: [Histonet] Fire in the lab > CC: histonet@lists.utsouthwestern.edu; > histonet-boun...@lists.utsouthwestern.edu > > Not exactly the same, but very similar. We had an automatic stainer > by the sink and one of the techs was washing glassware, the stainer > was running. The water apparently splashed on the wiring and a fire broke > out. We jumped into action. Just as we had been in-service. You are > correct what a mess to clean up! Fire extinquishers are wonderful but > extremely messy. We had totally taken care of the situation by the > time the fire department got here. We actually got accolades for > preventing a much larger fire. It was determined that there was some > exposed wires on the stainer. > A good lesson for all. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional > Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l > T: 804-765-5050 l F: > 804-765-5582 l dkb...@chs.net > > > > > > > > CHRISTIE GOWAN Sent by: > histonet-boun...@lists.utsouthwestern.edu > 02/26/2010 09:21 AM > > To > > cc > > Subject > [Histonet] Fire in the lab > > > > > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old > paraffin pot. One of my techs came in on Sunday to embed some tissues, > went into the processor room and smelled something burning. He noticed > our old paraffin pot had charred looking labels on the outside so he > went over, opened the lid and poof!!! the pot went up in flames. The > thermostat had gone haywire and heated the paraffin to flash point. > Opening the lid gave it the oxygen it needed to ignite. He triggered > the alarm, made the appropriate call and then put it out with an > extinguisher. Of course it kept re-igniting because he could not get > behind it to pull the plug. The fire dept finally was able to get it > pulled out and unplugged. Needless to say the tech was shaken and the > room was a mess. I applaud his courage and am not sure I would have > done the same. There was enough xylene and alcohol on the 4 processors > to cause quite an explosion but everything else was in a flammable > cabinet. I was wondering if this type of thing had ever happened to > anyone else?? Needless to say, we have de-comissioned all old paraffin > pots and will order only those with over temp safety features. I guess > I just wanted to remind everyone that fires can happen in the lab and > do probably more often than we hear about. This was the first time for > me and I have been in this business for over 20 years. Take care and be safe. > > Christie Gowan HT (ASCP) > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your > computer. Do not deliver, distribute or copy this message and do not > disclose its contents or take any action in reliance on the > information it contains. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _
RE: [Histonet] Fire in the lab
A few years back, I also had a fire break out while cleaning off the weighing instrument. There was left over powders of other chemicals on there and my gauze started on fire. So needless to say, please clean up after yourselves! It was Scary, very scary! I tried to yell for help but nothing would come out of my mouth, so I called 911. It was a lesson learned the hard way, for sure. Steph HT(ASCP) > To: christiego...@msn.com > From: dkb...@chs.net > Date: Fri, 26 Feb 2010 09:50:08 -0500 > Subject: Re: [Histonet] Fire in the lab > CC: histonet@lists.utsouthwestern.edu; > histonet-boun...@lists.utsouthwestern.edu > > Not exactly the same, but very similar. We had an automatic stainer by > the sink and one of the techs was washing glassware, the stainer was > running. The water apparently splashed on the wiring and a fire broke > out. We jumped into action. Just as we had been in-service. You are > correct what a mess to clean up! Fire extinquishers are wonderful but > extremely messy. We had totally taken care of the situation by the time > the fire department got here. We actually got accolades for preventing a > much larger fire. It was determined that there was some exposed wires on > the stainer. > A good lesson for all. > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkb...@chs.net > > > > > > > > CHRISTIE GOWAN > Sent by: histonet-boun...@lists.utsouthwestern.edu > 02/26/2010 09:21 AM > > To > > cc > > Subject > [Histonet] Fire in the lab > > > > > > > > > > Dear Histonet Friends, > > I just wanted to share an incident we recently had with an old paraffin > pot. One of my techs came in on Sunday to embed some tissues, went into > the processor room and smelled something burning. He noticed our old > paraffin pot had charred looking labels on the outside so he went over, > opened the lid and poof!!! the pot went up in flames. The thermostat had > gone haywire and heated the paraffin to flash point. Opening the lid gave > it the oxygen it needed to ignite. He triggered the alarm, made the > appropriate call and then put it out with an extinguisher. Of course it > kept re-igniting because he could not get behind it to pull the plug. The > fire dept finally was able to get it pulled out and unplugged. Needless to > say the tech was shaken and the room was a mess. I applaud his courage and > am not sure I would have done the same. There was enough xylene and > alcohol on the 4 processors to cause quite an explosion but everything > else was in a flammable cabinet. I was wondering if this type of thing had > ever happened to anyone else?? Needless to say, we have de-comissioned all > old paraffin pots and will order only those with over temp safety > features. I guess I just wanted to remind everyone that fires can happen > in the lab and do probably more often than we hear about. This was the > first time for me and I have been in this business for over 20 years. Take > care and be safe. > > Christie Gowan HT (ASCP) > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail: Trusted email with Microsoft’s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ruo antibodies
Our new CAP checklist does not mention the use of RUO antibodies anymore. This was under the question using ASR antibodies in the past. I believe the requirement was that if we wanted to use an RUO antibody we had to have a disclaimer similar to the ASR disclaimer but we also had to have a statement stating that we had searched for an IVD or ASR antibody. Does anyone know if this is still the practice or am I missing something? I do know of course than when using either an ASR or RUO antibody we have to establish and verify the performance. Any thoughts about the RUO(s)? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Saturday Tech Needed Orange County California
Hello histotechs- please email me if you're interested in working a few hours on Saturday mornings. We are flexible, and have another Saturday tech that can fill in if you can not work on certain dates. I'll give you more info privately. I'm only seeking histotechs who have at least 2 years experience embedding and cutting surgical/biopsy cases in a hospital or private lab setting. Thanks, Paula Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides
Hello, I'm glad that you already solved the problem your way. I didn't read your first post, but we had exactly the same problem (and we're also using cover plates). This artifact is very likely caused by tiny air bubbles which are trapped under the cover plate. The crucial step is when you drip a little buffer onto the plate in order to get your slide in proper position. You get much lesser air bubbles if a) no detergent is used and b) the PBS or TBS is at room temperature and not cold (which isn't good anyway). We don't use detergents anymore (on coverplates). Alexandra Meinl Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.me...@trauma.lbg.ac.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fire in the lab
Not exactly the same, but very similar. We had an automatic stainer by the sink and one of the techs was washing glassware, the stainer was running. The water apparently splashed on the wiring and a fire broke out. We jumped into action. Just as we had been in-service. You are correct what a mess to clean up! Fire extinquishers are wonderful but extremely messy. We had totally taken care of the situation by the time the fire department got here. We actually got accolades for preventing a much larger fire. It was determined that there was some exposed wires on the stainer. A good lesson for all. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net CHRISTIE GOWAN Sent by: histonet-boun...@lists.utsouthwestern.edu 02/26/2010 09:21 AM To cc Subject [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fire in the lab
Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides
Are you sure that you don't introduce air bubbles when you put your slides into the coverplates? The antibody will not touch the tissue if there is an air bubble. Willem Hoekert Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: do 25-2-2010 16:42 Aan: histonet@lists.utsouthwestern.edu; histonet.nos...@vneubert.com Onderwerp: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To me it seems that the sections after being picked from the water bath were not completely drained and the dewaxing process was incomplete in a way that the "round" areas kept certain amount of paraffin wax that prevented the reagents reactions. The fact that the areas are round are an indication that water was involved, since water always leave a round imprint, due to its surface tension. I would suggest that you dewax the sections with a 2% aq. solution of dish washer soap. Dewaxing with xylene sections containing water will be incomplete because it does not mix completely with water but the detergent will mix with the water and will better remove the paraffin.René J. --- On Thu, 2/25/10, histonet.nos...@vneubert.com wrote: From: histonet.nos...@vneubert.com Subject:>[Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides To: histonet@lists.utsouthwestern.edu Date: Thursday, February 25, 2010, 8:34 AM Hello Histonet, it has been a while (~10 months) since I posted a problem about uneven immuno-staining with specimen showing unstained circles after manual staining with HRP-polymere/DAB method; complete mail see below, response mails see Histonet archive (via website). Links to pictures I took: http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg The problem occured suddenly, without having changed any reagents or methods. Things I changed to avoid the unstained spots: *Adding 0,05% Tween 20 to TBS *Blocking peroxidase in coplin jar, not mounted in racks *Lowering antibody concentration which temporarily produced better results. After reviewing a big number of slides it showed up that most of the tissue affected was lung, liver and kidney which mostly means a lot of blood in the tissue when fixation in formalin starts. Erythrocytes, granulocytes and macrophages show a lot of endogenous and pseudoendogenous peroxidase activity. This is how it's done since then: Slides are taken from racks into a big coplin jar with 3% H2O2 diluted in distilled water (demineralized H2O). A slow magnetic stirrer on the bottom of the jar keeps the solution floating around the tissue, removing any O2 bubble that might appear. Slides then are remounted and rinsed a lot with TBS-T. Thank you for all your help, though it's a little late... V. Neubert, Germany - Original Message - From: histonet.nos...@vneubert.com To: histonet@lists.utsouthwestern.edu Date: 02.04.2009 18:12:18 Subject: [Histonet] Strange circles in IHC slides [...]> http://img12.imageshack.us/img12/8513/ts0402162049.jpg > http://img13.imageshack.us/img13/6514/ts0402162104.jpg [...]> > So, has ever anyone experienced sth. like this? > My conjugate control (every step except the antibody) was fine, nothing > to be seen about DAB and no circles at all. > > I used Shandon single-use coverplates, sterile buffer, fresh antibody > aliquots. Any idea? > > Thanks, > > V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: shandon's formal fixx and cytoblock kit
On Sat, Feb 20, 2010 at 3:54 PM, shehnaz khan wrote: > Hi Histonetters > > Could someone kindly share their views on the shandon's formal fixx > (concentrate) and cytoblock kit for cell block preparation in cytology? Has > it been effective for cellular preservation and cell capture from inadeduate > samples? > > Thanking you in advance. > > S .Khan > Dept of Cytology > University of Witwatersrand > Johannesburg > South Africa > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
