[Histonet] problem in IF stains

[Aazath Raj]

Dear Friends,

 I am having a problem in IF stains on Renal Bx.In direct 
immunofloresence on the frozen sections does not have disered contrast and many 
of the cases its falsely negative.

Can you people tell me the possible mistake which may cause these kind of 
effects on the IF stain.

I dont see any gross mistakes on the Frozen sections after sectioning.All the 
temperature and other things are fine on the cryostate, but my pathologist 
feels that there may be any problem in section.will that have any effects on 
the IF stains..

 

Aazathraj.

Technical Officer.

Apollo Hospitals

India.
  
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RE: [Histonet] Superfrost Plus Slides

[Cathy]

Hi Claire,

We experienced problems with our IHC staining the fall of 2008 and it was
traced back to a problem with particular lot numbers of the Superfrost
slides.  It was difficult to track down due to the inconsistency of the
problem and the variation in the problem.  We finally spoke with our Ventana
technical specialist and she was able to tie our problem to problems that
other facilities were experiencing.  
You could try contacting your Ventana technical resource person; they may be
able to help.

One thing we found out was that the Superfrost slides are manufactured at
one site and distributed to vendors under their own name.

Cathy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fahy, Claire
Sent: Tuesday, March 16, 2010 4:05 AM
To: Lynn Lee
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Superfrost Plus Slides

Hi Lynn

Yes, our instrument is decontaminated and cleaned as per manual.

There was some old threads on Histonet where some people had got varying
staining between runs so I was wondering if anybody had found a solution to
this problem.Maybe it is simply the slides?
-Original Message-
From: Lynn Lee [mailto:lynnlee2...@live.com]
Sent: 16 March 2010 10:43
To: Fahy, Claire
Subject: RE: [Histonet] Superfrost Plus Slides

You could have contamination in the instrument. Has it been decontaminated
quarterly per the instruction manual? I have never heard of uneven staining
from plus slides.






> Date: Tue, 16 Mar 2010 10:16:38 +
> From: claire.f...@agriculture.gov.ie
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Superfrost Plus Slides
>
> Hi
>
> I know this is in reply to an old threat but we are experiencing varying
and uneven staining of our sections on the Ventana Benchmark.It is not
confined to a perticular anitbody and doesn't happen all the time either.
>
> We have been told that it may be the slides.We currently use Thermo
Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the
Ventana IHC slides?
>
> Has anyone has experienced similar problems?If so,what have you done to
resolve them.
>
> Thanks
>
> Claire fahy
> Lab analyst,
> Histopatholgy Lab
>
> Department of Agriculture, Fisheries and Food
>
> The information contained in this email and in any attachments is
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tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air,
nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat
de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar
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[John Kiernan]

There is
Garvey,W (1991): Modification of the Mayer hematoxylin stain. J. Histotechnol. 
14, 163-165.
The title is rather confusing. Her method included a modified composition of 
Mayer's haemalum (ethanol instead of chloral hydrate, and less alum), and 
instead of eosin Y the counterstain was a solution containing phloxin B 
(CI45410, Acid red 92) and saffron (which is CI 75100, Natural yellow 6). The 
author stated that saffron bought from a health food store was OK and much less 
expensive than that bought from a lab vendor.
 This counterstain was used by Rostoker et al (2001) Nephrology Dialysis 
Transplantation 16:513-517 in a study of intestinal pathology in 
glomerulonephritis.

John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: sheila adey 
Date: Tuesday, March 16, 2010 15:05
Subject: [Histonet] Saffron/Eosin as a counterstain for H&E???
To: histonet@lists.utsouthwestern.edu

> 
> Hello,
> 
> Our pathologist said that he say H&E slides with a counterstain 
> that had some amount of saffron in it?
> 
> Is anyone familiar with this? 
> 
> Sheila Adey HT MLT 
> 
> 
>
> _
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Re: [Histonet] Spencer AO 820 microtome questions

[Michael Folsom]

Scott:

Does the chuck have a clamp in it?  If so soak a piece of soft wood that
fits in the chuck in 100% ETOH (pulling a vacuum on it would help) and
transition into paraffin.  Once its soaked in paraffin pull it out and
allow to solidify.  Your tissue will need to be flat embedded in a
simple boat.  Use a razor blade to cut it out but leave a good bit of
paraffin behind and around it. Once its cool use an artists pallet knife
(small) to melt the surface of the paraffin on the wood and the back of
the block and weld in place with the melted paraffin.

Re: the knife - they make a knife holder that holds either hi or low
profile disposable blades which fits in the microtome's knife holder.

One thing about the old AO - they are almost indestructible but never
back up the crank because it can strip the gears.

Good luck -


Mike
mwfol...@rgbio.cm



On Tue, 2010-03-16 at 14:20 -0400, Scott Parker wrote:
> Dear all,
> 
> I have access to a Spencer AO 820 microtome (a "black beauty" in
> Histo-parlance)  that is does not have a disposable blade holder nor a chuck
> for holding paraffin cassettes. The microtome otherwise appears to be in
> good condition. I would like to get suggestions for where I might be able to
> purchase a chuck and disposable blade holder for this classic model.
> Alternatively, would it be a better idea for me to put this piece of
> equipment on display in a glass case and instead purchase a newer microtome.
> My work involves relatively low volume, basic sectioning of paraffin blocks
> for teaching and research. I don't need anything too fancy, just a microtome
> that is reliable and appropriate for student researchers to use.
> 
> Thank you in advance for your responses.
> 
> Scott
> 
> 
> Scott L. Parker, Ph.D.
> 
> Assistant Professor Biology
> 
> Coastal Carolina University
> 
> P.O. Box  261954
> 
> Conway, SC 29528-6054
> 
> 
> 
> Office Phone: (843)-349-2491
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RE: [Histonet] (no subject)

[WILLIAM DESALVO]

An additional method to use to create positive identification when accessioning 
and grossing like specimens together when you must or just to increase the 
indetification factor for all specimens: 

 

Use the Davidson marking system and have the grossing person rotate the colors 
used to mark margins or small tissue by case and/or specimen and of course 
dictate to specific color used. Once they are in the habit, no two cases or 
specimens will have the same ink color and you increase quality assurance.

William DeSalvo, B.S., HTL(ASCP)




 
> Date: Tue, 16 Mar 2010 14:23:45 -0700
> From: pathmas...@yahoo.com
> To: allison_sc...@hchd.tmc.edu
> CC: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
> 
> Hi Allison- 
> That's our policy- don't accession like biopsies back to back. 
> But if it is unavoidable, you can mark the second like biopsy case with a dab 
> of yellow margin ink on at least one piece in each block and dictate that 
> into the gross.  ie One fragment is marked in yellow for positive patient 
> identification.
> 
> Jeff Silverman- busy PA
> 
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RE: [Histonet] (no subject)

[Sherwood, Margaret]

We also just wash in running tap water and the yellow stain disappears. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey
Silverman
Sent: Tuesday, March 16, 2010 5:15 PM
To: cscam...@uci.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Don't know what you mean by rinses, but 5 to at most 10 minutes in gently
running tap water should do the trick. If not, the lithium carbonate is what's
prescribed. 

Jeff Silverman

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Re: [Histonet] Removing Yellow color from slides refixed in Bouin's solution

[Joseph Saby]

Running tap water for 10 minutes should do the trick.

Joe Saby





From: Cynthia Pyse 
To: cscam...@uci.edu; HistoNet 
Sent: Tue, March 16, 2010 10:52:20 AM
Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's 
solution

I just place the slides into 80% ETOH for 2 minutes.
Cindy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
cscam...@uci.edu
Sent: Monday, March 15, 2010 7:42 PM
To: HistoNet
Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's
solution

Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water are not
doing the trick. Does anyone have some advice on how to bring the slides
back to a clear color so that I may proceed with the Masson Trichrome
procedure?

Thanks!
-Colin


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[Histonet] (no subject)

[Jeffrey Silverman]

Hi Allison- 
That's our policy- don't accession like biopsies back to back. 
But if it is unavoidable, you can mark the second like biopsy case with a dab 
of yellow margin ink on at least one piece in each block and dictate that into 
the gross.  ie One fragment is marked in yellow for positive patient 
identification.

Jeff Silverman- busy PA

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[Histonet] CAP cancer protocols and checklists

[Carol Bryant]

I am interested in how you are implementing the CAP cancer protocols in your 
laboratory?  Are you using synoptic reporting, dictating from the checklists, 
etc?  Any information you can provide would be greatly appreciated.
Thank you in advance for your comments and input.

Carol

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cb...@lexclin.com



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[Histonet] (no subject)

[Jeffrey Silverman]

Don't know what you mean by rinses, but 5 to at most 10 minutes in gently 
running tap water should do the trick. If not, the lithium carbonate is what's 
prescribed. 

Jeff Silverman

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[Histonet] NY License

[Jeffrey Silverman]

New York State Education Department- google it and you'll see a link for 
licensed professionals. 

Jeff Silverman

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histonet@lists.utsouthwestern.edu

[Liz Chlipala]

Its hematoxylin, ploxine and saffron.  Believe it or not it was the
stain that we used at my first job back in 1973.  We also fixed in
Zenkers too.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sheila
adey
Sent: Tuesday, March 16, 2010 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Saffron/Eosin as a counterstain for H&E???


Hello,

Our pathologist said that he say H&E slides with a counterstain that had
some amount of saffron in it?

Is anyone familiar with this? 

Sheila Adey HT MLT 


  
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histonet@lists.utsouthwestern.edu

[sheila adey]

Hello,

Our pathologist said that he say H&E slides with a counterstain that had some 
amount of saffron in it?

Is anyone familiar with this? 

Sheila Adey HT MLT 


  
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[Histonet] Lab Assistant Position in Phoenix, AZ

[Turner, Mark]

 

Caris MPI, a division of Caris Life Sciences, is looking to hire a Lab
Assistant for 2nd shift.  This will be a support position for our team
of histology professionals, and is a great entry level position into the
laboratory setting.  Some college is preferred, with a focus on the
sciences.  Opportunities for advancement abound, as this position is
open due to our previous lab assistant moving into a histotech role.

 

Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand
new lab in May 2010.  Equipment is state of the art, and there is a
corporate commitment toward quality.   Caris has had a presence in the
Phoenix area for 3 years and is continuing to grow.  The area is the 5th
largest metropolitan in the country and has available anything you need
to make life more interesting.

 

If you would like to grow with us, please submit your resume in
confidence to mtur...@carisdx.com.

 

 

Mark Turner, Ph. D., HT(ASCP) QIHC

Supervisor IHC

Target Now MPI

 

Caris Life Sciences

445 N. 5th Street

Phoenix, AZ  85004

 

Cell:   602-309-5084

Direct  602-358-8913

Fax:  602-358-8919

 

mtur...@carisdx.com

 

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[Histonet] Job Openings in Phoenix, Arizona

[Turner, Mark]

Hello Histonetters!

 

Caris MPI, a division of Caris Life Sciences, is looking for 2
histotechs to assist with a dramatically increasing workflow.  We are a
very dynamic IHC lab which is growing exponentially.  The positions are
day shift, Monday through Friday, with occasional weekends.  The
successful candidates will have HT (ASCP) certification, 1-2 years IHC
experience, preferably with the Ventana and Dako platforms, and will
enjoy being part of a fast paced, quality oriented team.  Qualification
in Immunohistochemistry (QIHC) is a plus.

 

Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand
new lab in May 2010.  Equipment is state of the art, and there is a
corporate commitment toward quality.   Caris has had a presence in the
Phoenix area for 3 years and is continuing to grow.  The area is the 5th
largest metropolitan in the country and has available anything you need
to make life more interesting.

 

If you would like to grow with us, please submit your resume in
confidence to mtur...@carisdx.com.

 

 

 

Mark Turner, Ph. D., HT(ASCP) QIHC

Supervisor IHC

Target Now MPI

 

Caris Life Sciences

445 N. 5th Street

Phoenix, AZ  85004

 

Cell:   602-309-5084

Direct  602-358-8913

Fax:  602-358-8919

 

mtur...@carisdx.com

 

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[Histonet] Spencer AO 820 microtome questions

[Scott Parker]

Dear all,

I have access to a Spencer AO 820 microtome (a "black beauty" in
Histo-parlance)  that is does not have a disposable blade holder nor a chuck
for holding paraffin cassettes. The microtome otherwise appears to be in
good condition. I would like to get suggestions for where I might be able to
purchase a chuck and disposable blade holder for this classic model.
Alternatively, would it be a better idea for me to put this piece of
equipment on display in a glass case and instead purchase a newer microtome.
My work involves relatively low volume, basic sectioning of paraffin blocks
for teaching and research. I don't need anything too fancy, just a microtome
that is reliable and appropriate for student researchers to use.

Thank you in advance for your responses.

Scott


Scott L. Parker, Ph.D.

Assistant Professor Biology

Coastal Carolina University

P.O. Box  261954

Conway, SC 29528-6054



Office Phone: (843)-349-2491
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[Histonet] Re: proficiency testing for Muscle EHC- cont'd.

[Barone, Carol]

Histonetter's I only received one reply on my question regarding "what
everyone is doing for proficiency testing of enzyme HC staining for
muscle"...espeically for CLIA.compliance, now that I will lose my
indepent reviewer. 

 My thanks to Jan Minchew for her reply. I am working from that for now,
though my sitiation somewhat different. .but, I am finding it
particularly frustrating finding an answer to this question over the
last three months or more, that I have searched everywhere from the
histonet to the internet toevery friend I have doing EHC. Is it required
under CLIA? My auditor says yes..so I am trying to comply, though I
cannot fiind it specifically in any CLIA reg.


Who is the CLIA expert out there...and why is this not included in HQIP?
Can someone shed more light. Thx. CB
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[Histonet] HT/HTL position Gainesville. FL

[Konni Black]

Fulltime HT/HTL opening in GI lab in Gainesville, Florida. Must have 3-5 years 
experience. Great lab. Flexible work schedule. Contact Konni Black at 
kbl...@digestivehlth.com or 253-503-2560. 
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Re: [Histonet] UNSUBSCRIBE

[Kim . Donadio]

I have a suggestion. If you are extremely frustrated with getting all the 
emails that come from here and you are having a hard time unsubscribing, 
perhaps you could put a block on histonet so you cant receive emails from 
them until you get unsubscribed?  Just a thought.






Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



"Vanessa Avalos"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/16/2010 12:01 PM

To
"'HISTONET LISTS'" 
cc

Subject
[Histonet] UNSUBSCRIBE






I can only speak for myself but yes, I am aware of all steps to be taken 
in unsubscribing. Have followed them more than once but have been 
unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS! 
:)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily 
Sours
Sent: Tuesday, March 16, 2010 7:53 AM
To: Dimitri Scholz
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

both of you are not allowed to use the internet anymore.
because you can't follow instructions.
WE CAN'T UNSUBSCRIBE YOU.  ONLY YOU CAN.


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at pistol-point.  We must fight for variety if we fight at all.  The
uniform is as dull as a sculptured egg.
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On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz  
wrote:
> Me too!
>
> Dimitri Scholz, PhD, Dr. Sci.
> Director of Biological Imaging
> Conway Institute
> University College Dublin (UCD)
> Belfield, Dublin 4
> Ireland
> Tel: +353-1 716 6736
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa
> Gutierrez
> Sent: 16 March 2010 14:21
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND 
STILL AM
> ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING 
THE
> RULES GETS ME OFF!
>
> THANK YOU
>
>
>
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recipient, please take notice that any use, copying, printing,
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[Histonet] Exciting Histotech Job in New England!!

[Alisha Dynan]

Hi Histotechs,






















I am a recruiter at a highly successful and well respected Healthcare 
recruiting firm.  I help place Lab Professionals in permanent positions across 
the country and I wanted to see if you are interested in exploring other career 
opportunities?  We are completely free of charge to candidates and and we work 
on quite a few laboratory openings across the country. Our clients typically 
assist with relocation expenses. I am currently working on some great job 
opportunities in various areas across the country.

One particular client that I am working with is a financially sound, medically 
advanced, full-service hospital in New England. My client is looking for both a 
HTL(ASCP) certified histotechnologist and a HT(ASCP) certified histotech for 
their hospital. Both positions would work a day shift. My client is offering a 
very competitive compensation package, full benefits, and relocation 
assistance. If interested, please email me a copy of your resume and follow-up 
with a call to learn more!

Below is a list of some of the great opportunities we are currently working on. 
If you do not see an opening in a location in which you live or would like to 
live, please send me an email me a copy of your resume and let me know where 
you would be interested in a job. I will then tailor a search for you that is 
completely confidential and free to candidates.



Current Opportunities: 



CT - Histology Operations Manager


NY - New York City - Histotech 3rd shift


NV -Las Vegas - Histotech 3rd shift


NV Las Vegas - Histology Supervisor - 3rd shift


PA - Cytology Supervisor


CA - Palm Springs - Histotech - 1st shift


CA - Southern - Histotech


VA - Histotech 2nd shift


NH - HTL and HT


GA - Atlanta - Histotech


GA - Atlanta - Pathology Coordinator (HT or CT)


NV - Pathologist Assistant


TN - Pathologist Assistant


ND - Pathologist Assistant


GA - Pathologist Assistant

























If you're interested in learning more about these opportunities or 
opportunities in a certain geographic location please reply with an updated 
resume and let me know when a good time to reach you is.  

If this is not the right fit for you please let me know who you can recommend 
and give me an idea of what types of positions you'd be interested in hearing 
about in the future.  I cover the entire US and have am working on Lab 
positions at all levels. We offer a very generous referral bonus for anyone you 
refer to us that we place into any position across the country.  


To view some additional opportunities please visit our website at 
www.ka-recruiting.com.  
































Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

ali...@ka-recruiting.com

www.ka-recruiting.com

 






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[Histonet] UNSUBSCRIBE

[Vanessa Avalos]

I can only speak for myself but yes, I am aware of all steps to be taken in 
unsubscribing. Have followed them more than once but have been unsuccessful. So 
yes, my frustration as lead me to write IN ALL CAPS!  :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, March 16, 2010 7:53 AM
To: Dimitri Scholz
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

both of you are not allowed to use the internet anymore.
because you can't follow instructions.
WE CAN'T UNSUBSCRIBE YOU.  ONLY YOU CAN.


A state-imposed metaphysic or religion should be opposed, if necessary
at pistol-point.  We must fight for variety if we fight at all.  The
uniform is as dull as a sculptured egg.
--Lawrence Durrell, Consequential Data, "Balthazar"



On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz  wrote:
> Me too!
>
> Dimitri Scholz, PhD, Dr. Sci.
> Director of Biological Imaging
> Conway Institute
> University College Dublin (UCD)
> Belfield, Dublin 4
> Ireland
> Tel: +353-1 716 6736
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa
> Gutierrez
> Sent: 16 March 2010 14:21
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM
> ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE
> RULES GETS ME OFF!
>
> THANK YOU
>
>
>
> ___
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>
>
> ___
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FW: [Histonet] How to unsubscribe

[Vanessa Avalos]

Oh yes, I have gone to the link, gone to the home page and followed all of
the instructions for unsubscribing. Replied with the code it gives you, have
been given a confirmation email and still I am flooded with this. Have done
that twice and posted it three times now.  I added my work email to this and
want my personal one off. I enjoy reading about all the topics but not
twice. Lol. Thanks anyway.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor
Tobias
Sent: Tuesday, March 16, 2010 7:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How to unsubscribe

Vanessa,

Did you click on this link at the bottom of every email?

http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Once you get to the page scroll down until you see;

To unsubscribe from Histonet, get a password reminder, or change your 
subscription options enter your subscription email address:


Enter your email address and press unsubscribe.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


On 3/16/2010 7:21 AM, Vanessa Gutierrez wrote:
> UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL
AM ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING
THE RULES GETS ME OFF!
>
> THANK YOU
>
>
>
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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[Histonet] Accessioning and Grossing Alike Specimens

[Scott, Allison D]

What policies do you have in place for accessioning and grossing
specimens that are alike, especiall biopsy specimens. It has been my
experience that you should not have alike specimens back to back.  

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital 
5656 Kelley 
Houston, Texas 77026
713-566-5287

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

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[Histonet] Fwd: spinal cord paraffin embedding

[Elisa Ballarini]

   Hi.
   First of all I would like to thank all of you for your advices.
   Because,  my  previous  e-mail  wasn't  complete  and to avoid further
   suggestions  regarding  protocols  I can not follow, here I write some
   more information.
   First  of  all,  we  can't perfuse our rats. Also, when we harvest rat
   spinal cord (cut in half, thus around 3mm diameter) we fix it only for
   4-5  hours  in  4%  paraformaldehyde because we need to perform an IHC
   essay so we would like to avoid epitope masking.
   We  don't  think  fixation is the problem since frozen sections of the
   same fixed spinal cords do not show any problem in the white matter.
   In  conclusion,  we need to obtain good results using paraffin both in
   term= s of morphology and IHC staining.
   Our paraffin embedding machine program is as follows:
   Ethanol 95%   1h
   Ethanol 95%   45'
   Ethanol 95%   45'
   Ethanol 100%  45'
   Ethanol 100%  1h
   Ethanol 100%  1h
   Ethanol 100%  1h
   Xylol 45'
   Xylol 1h
   Xylol 1h
   Paraffin 1h
   Paraffin 1h
   Paraffin 1h
   Can  you  tell  me  if  are there any specific protocols to embedd rat
   spinal = cord in paraffin to avoid washing out of myelin?
   Thank you.
   Regards
   --
   -- 
   Dott.ssa Elisa Ballarini,PhD student
   Dipartimento di Neuroscie= nze e Tecnologie Biomediche
   Università degli Studi Milano-Bicocca
   Via Cadore 48-20052,Monza,MB
   e.ballari...@campus.uni= mib.it
   Tel. 02-64488119
   Fax. 02-64488253

--- segue il m= essaggio inoltrato ---
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Re: [Histonet] Unsubscribe please

[Adam .]

I think a lot of grief could be saved if the managers of HistoNet added the
following four words at the bottom of each e-mail. Who would I need to
contact to make such a suggestion?

http://lists.utsouthwestern.edu/mailman/listinfo/histonet to change
subscription preferences

Adam

On Tue, Mar 16, 2010 at 10:08 AM, Emily Sours  wrote:

> JUPITER'S THUNDER!
> GO TO THIS WEBSITE.
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> SCROLL DOWN TO THE BOX WHERE IT SAYS UNSUBSCRIBE (MAKE SURE YOU USE
> THE CORRECT BOX OR YOU WILL NOT BE UNSUBSCRIBED) AND TYPE IN YOUR
> EMAIL.
> CLICK ON THE BOX THAT SAYS UNSUBSCRIBE.
> DO NOT WRITE TO THE LIST.
>
> A state-imposed metaphysic or religion should be opposed, if necessary
> at pistol-point.  We must fight for variety if we fight at all.  The
> uniform is as dull as a sculptured egg.
> --Lawrence Durrell, Consequential Data, "Balthazar"
>
>
>
> On Tue, Mar 16, 2010 at 10:57 AM, Barbara Ratner  wrote:
> >
> >
> > Barbara Ratner
> > Vice President
> > Merraine Group Inc.
> > One Executive Blvd.
> > Suite #110
> > Montebello, NY 10901
> >
> > direct: (845) 290-1900
> > main: (845) 357-3355 x104
> > fax (212) 918-9184
> > e-mail: b...@merraine.com  or b...@sensationaltalent.com
> > www.merraine.com 
> >
> > "Providing Leaders for Healthcare & Energy"
> > Domestic & International Placements
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
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[Histonet] the spammer is back!

[Geoff McAuliffe]

Dear all:

aa aa is a troll/spammer. Do not reply to his attempts to cause trouble, 
it only encourages him.


Geoff


aa aa wrote:

How Do I unsuscrive from this list please



  
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--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcaul...@umdnj.edu

**



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[Histonet] UNSUSCRIVE REQUIST

[aa aa]

How Do I unsuscrive from this list please



  
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Re: [Histonet] Unsubscribe please

[Emily Sours]

JUPITER'S THUNDER!
GO TO THIS WEBSITE.
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
SCROLL DOWN TO THE BOX WHERE IT SAYS UNSUBSCRIBE (MAKE SURE YOU USE
THE CORRECT BOX OR YOU WILL NOT BE UNSUBSCRIBED) AND TYPE IN YOUR
EMAIL.
CLICK ON THE BOX THAT SAYS UNSUBSCRIBE.
DO NOT WRITE TO THE LIST.

A state-imposed metaphysic or religion should be opposed, if necessary
at pistol-point.  We must fight for variety if we fight at all.  The
uniform is as dull as a sculptured egg.
--Lawrence Durrell, Consequential Data, "Balthazar"



On Tue, Mar 16, 2010 at 10:57 AM, Barbara Ratner  wrote:
>
>
> Barbara Ratner
> Vice President
> Merraine Group Inc.
> One Executive Blvd.
> Suite #110
> Montebello, NY 10901
>
> direct: (845) 290-1900
> main: (845) 357-3355 x104
> fax (212) 918-9184
> e-mail: b...@merraine.com  or b...@sensationaltalent.com
> www.merraine.com 
>
> "Providing Leaders for Healthcare & Energy"
> Domestic & International Placements
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

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[Histonet] Question Salary Compensation

[Alyssa Peterson]

Hello,

I am not sure if this is too far off of subject here. However, I have a
client in NYC, brand new private lab who is looking into hiring Sales Reps
to aquire more clients to read slides for, within his Pathology Lab. Does
anyone out there in histoland have an idea of a Sales Reps Salary working
with  Private Pathology Laboratory?

Thank you in advance for any help.

-Alyssa
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[Histonet] Unsubscribe please

[Barbara Ratner]

 
 
Barbara Ratner
Vice President
Merraine Group Inc.
One Executive Blvd.
Suite #110
Montebello, NY 10901
 
direct: (845) 290-1900
main: (845) 357-3355 x104
fax (212) 918-9184
e-mail: b...@merraine.com  or b...@sensationaltalent.com
www.merraine.com  
 
"Providing Leaders for Healthcare & Energy"
Domestic & International Placements 
 
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RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution

[Cynthia Pyse]

I just place the slides into 80% ETOH for 2 minutes.
Cindy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
cscam...@uci.edu
Sent: Monday, March 15, 2010 7:42 PM
To: HistoNet
Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's
solution

Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water are not
doing the trick. Does anyone have some advice on how to bring the slides
back to a clear color so that I may proceed with the Masson Trichrome
procedure?

Thanks!
-Colin


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Re: [Histonet] (no subject)

[Emily Sours]

both of you are not allowed to use the internet anymore.
because you can't follow instructions.
WE CAN'T UNSUBSCRIBE YOU.  ONLY YOU CAN.


A state-imposed metaphysic or religion should be opposed, if necessary
at pistol-point.  We must fight for variety if we fight at all.  The
uniform is as dull as a sculptured egg.
--Lawrence Durrell, Consequential Data, "Balthazar"



On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz  wrote:
> Me too!
>
> Dimitri Scholz, PhD, Dr. Sci.
> Director of Biological Imaging
> Conway Institute
> University College Dublin (UCD)
> Belfield, Dublin 4
> Ireland
> Tel: +353-1 716 6736
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa
> Gutierrez
> Sent: 16 March 2010 14:21
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM
> ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE
> RULES GETS ME OFF!
>
> THANK YOU
>
>
>
> ___
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>
>
> ___
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>

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Re: [Histonet] JOH Article Question

[Rene J Buesa]

If you are a member of the NSH you can ask them for a "pdf" of this, or any 
other article published in the JOH.
René J.

--- On Mon, 3/15/10, Amanda Woolsey  wrote:


From: Amanda Woolsey 
Subject: [Histonet] JOH Article Question
To: "histonet@lists.utsouthwestern.edu" 
Cc: "gcal...@montana.edu" 
Date: Monday, March 15, 2010, 11:27 PM


To Whom It May Concern,

I am trying to contact Gayle Callis about the article she refers to in the text 
below.
I would appreciate any information as I am trying to obtain a copy of this 
article.
(I have already tried through the NSH website and I am just trying all of the 
avenues!)

Thank you,

Amanda Woolsey, HTL
Histology - Pathology
Primary Children's Medical Center
Salt Lake City, Utah



[Histonet] growing gram pos and neg
Gayle Callis gcallis <@t> montana.edu
Mon Aug 20 10:30:38 CDT 2007

    * Previous message: [Histonet] growing gram pos and neg
    * Next message: [Histonet] Safran du Gatinais
    * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Jennifer,

There is a wonderful publication on making a combined Gram negative and 
Gram positive control in J of Histotechnology, December 2006.   The author 
is Kristine J. Vaughn (do not have email contact here) but I will CC to my 
home email so I can put you in contact with her.  She has reprints 
available, and will be delighted to send you one.  Her method is simple and 
very effective, plus addresses biosafety issues with human tissue use.   If 
you are a member of NSH, you can contact JOH and request this reprint free 
of charge too.  Go to the NSH website, then click on JOH for this request.

     At 04:57 PM 8/17/2007, you wrote:
>Hello all,
>
>I would like to hear your ideas on the best way to grow gram positive
>and negative organisms in tissue. It would have to be tissue that is not
>fixed so, what do you use? Did you grow the organisms, inject into
>tissue and then put into incubator? or do you grow organisms on agar and
>place tissue on it and incubate? or in a broth? Thank you for your
>input.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610





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[Histonet] How to unsubscribe

[Victor Tobias]

Vanessa,

Did you click on this link at the bottom of every email?

http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Once you get to the page scroll down until you see;

To unsubscribe from Histonet, get a password reminder, or change your 
subscription options enter your subscription email address:



Enter your email address and press unsubscribe.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


On 3/16/2010 7:21 AM, Vanessa Gutierrez wrote:

UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM ON 
THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE RULES 
GETS ME OFF!

THANK YOU



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RE: [Histonet] (no subject)

[Dimitri Scholz]

Me too!

Dimitri Scholz, PhD, Dr. Sci.
Director of Biological Imaging
Conway Institute 
University College Dublin (UCD)
Belfield, Dublin 4 
Ireland
Tel: +353-1 716 6736


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa
Gutierrez
Sent: 16 March 2010 14:21
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM
ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE
RULES GETS ME OFF! 

THANK YOU



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[Histonet] Inquiry

[Nita Searcy]

Anyone know of a company that performs microarrays commercially? 

Thanks
Nita

Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438

BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
END:VCARD

BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
END:VCARD

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[Histonet] (no subject)

[Vanessa Gutierrez]

UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM ON 
THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE RULES 
GETS ME OFF! 

THANK YOU



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Re: [Histonet] Removing Yellow color from slides refixed in Bouin's solution

[Geoff McAuliffe]

A few drops of saturated aqueous lithium carbonate added to the 70% 
alcohol step will do the job nicely.


Geoff

cscam...@uci.edu wrote:

Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water are not
doing the trick. Does anyone have some advice on how to bring the slides
back to a clear color so that I may proceed with the Masson Trichrome
procedure?

Thanks!
-Colin


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--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcaul...@umdnj.edu

**



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[Histonet] Histology Management Openings, Northeast

[Jonah Levinger]

To fellow histoneters: 

 

Our recruitment firm, Merraine Group Inc., specializes in Laboratory and
Pathology Administration.  We have been retained on two exclusive Histology
searches.  Feel free to forward this information to your colleagues. 

 

Histology Supervisor: Large hospital in New York City.  Two years of
supervisory experience.  Salary: $75,000 to 90,000 per year.

 

Manager Anatomic Pathology: Large hospital in Maryland/Virginia region.
Four years management experience.  Salary: $95-110k per year.

 

Jonah Levinger, Team Leader

Medical Recruitment

Merraine Group Inc.

(845) 357-3355 Ex. 108
j...@merraine.com

www.merraine.com 

 

 

 
https://www.logoworks.com/uploads/projects/183596601953/finalartwork/183596_
logo_final.jpg
 

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Jonah Levinger
Team Leader, Medical Recruitment
Wk:845.357.3355x108 Main:845.290.1900
Fax: 212.918.9184
  j...@merraine.com
  www.merraine.com

 

 
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RE: [Histonet] Superfrost Plus Slides

[Fahy, Claire]

Hi Lynn

Yes, our instrument is decontaminated and cleaned as per manual.

There was some old threads on Histonet where some people had got varying 
staining between runs so I was wondering if anybody had found a solution to 
this problem.Maybe it is simply the slides?
-Original Message-
From: Lynn Lee [mailto:lynnlee2...@live.com]
Sent: 16 March 2010 10:43
To: Fahy, Claire
Subject: RE: [Histonet] Superfrost Plus Slides

You could have contamination in the instrument. Has it been decontaminated 
quarterly per the instruction manual? I have never heard of uneven staining 
from plus slides.






> Date: Tue, 16 Mar 2010 10:16:38 +
> From: claire.f...@agriculture.gov.ie
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Superfrost Plus Slides
>
> Hi
>
> I know this is in reply to an old threat but we are experiencing varying and 
> uneven staining of our sections on the Ventana Benchmark.It is not confined 
> to a perticular anitbody and doesn't happen all the time either.
>
> We have been told that it may be the slides.We currently use Thermo 
> Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana 
> IHC slides?
>
> Has anyone has experienced similar problems?If so,what have you done to 
> resolve them.
>
> Thanks
>
> Claire fahy
> Lab analyst,
> Histopatholgy Lab
>
> Department of Agriculture, Fisheries and Food
>
> The information contained in this email and in any attachments is 
> confidential and is designated solely for the attention and use of the 
> intended recipient(s). This information may be subject to legal and 
> professional privilege. If you are not an intended recipient of this email, 
> you must not use, disclose, copy, distribute or retain this message or any 
> part of it. If you have received this email in error, please notify the 
> sender immediately and delete all copies of this email from your computer 
> system(s).
>
> An Roinn Talmhaíochta, Iascaigh agus Bia
>
> Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi 
> phribhléid agus faoi rún agus le h-aghaigh an seolaí amháin. D’fhéadfadh 
> ábhar an seoladh seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura 
> tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air, 
> nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat 
> de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar 
> ó do ríomhaire le do thoil.
>
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Department of Agriculture, Fisheries and Food

The information contained in this email and in any attachments is confidential 
and is designated solely for the attention and use of the intended 
recipient(s). This information may be subject to legal and professional 
privilege. If you are not an intended recipient of this email, you must not 
use, disclose, copy, distribute or retain this message or any part of it. If 
you have received this email in error, please notify the sender immediately and 
delete all copies of this email from your computer system(s).

An Roinn Talmhaíochta, Iascaigh agus Bia

Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi phribhléid 
agus faoi rún agus le h-aghaigh an seolaí amháin. D’fhéadfadh ábhar an seoladh 
seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura tusa an seolaí a bhí 
beartaithe leis an ríomhphost seo a fháil, tá cosc air, nó aon chuid de, a 
úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat de bharr dearmad, 
téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar ó do ríomhaire le do 
thoil.
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[Histonet] Superfrost Plus Slides

[Fahy, Claire]

Hi

I know this is in reply to an old threat but we are experiencing varying and 
uneven staining of our sections on the Ventana Benchmark.It is not confined to 
a perticular anitbody and doesn't happen all the time either.

We have been told that it may be the slides.We currently use Thermo Scientific 
(Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides?

Has anyone has experienced similar problems?If so,what have you done to resolve 
them.

Thanks

Claire fahy
Lab analyst,
Histopatholgy Lab

Department of Agriculture, Fisheries and Food

The information contained in this email and in any attachments is confidential 
and is designated solely for the attention and use of the intended 
recipient(s). This information may be subject to legal and professional 
privilege.  If you are not an intended recipient of this email, you must not 
use, disclose, copy, distribute or retain this message or any part of it. If 
you have received this email in error, please notify the sender immediately and 
delete all copies of this email from your computer system(s).

An Roinn Talmhaíochta, Iascaigh agus Bia

Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi phribhléid 
agus faoi rún agus le h-aghaigh an seolaí amháin. D’fhéadfadh ábhar an seoladh 
seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura tusa an seolaí a bhí 
beartaithe leis an ríomhphost seo a fháil, tá cosc air, nó aon chuid de, a 
úsáid, a chóipeál, nó a scaoileadh.  Má tháinig sé chugat de bharr dearmad, 
téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar ó do ríomhaire le do 
thoil.

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