Re: [Histonet] Anti-human abs work on porcine tissue
I routine use anti-human antibodies to stain mouse tissue, so it can be done. It completely depends on the antigen they used to immunize. If the human and pig proteins are highly homologous or identical, then it has a good chance of working. If they're not, you're in uncharted territories. If you ask nicely, most companies will tell you what antigen or antigen fragment they used, but most don't actively advertise this. Adam On Wed, Mar 17, 2010 at 8:15 PM, RICKY MATHIS wrote: > I work with some porcine tissues and it is sometimes difficult to find > antibodies specific to pig. A doctor I work with asked some one he knew in > Europe about using antibodies that are listed as working in human being used > on porcine tissue. This person stated that there would be about a 10% > chance that the anti-human antibodies would work on porcine tissues. The > antibody companies mostly give the same "we did not try it on pig tissue" > response. I understand that it is likely expensive to continue to test > antibodies on a wide variety of animal tissues, so that is fine. But what > do you guys think about the 10% chance? I would have said more than that. > Thank you in advance for your time, > Cathy > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anti-human abs work on porcine tissue
I work with some porcine tissues and it is sometimes difficult to find antibodies specific to pig. A doctor I work with asked some one he knew in Europe about using antibodies that are listed as working in human being used on porcine tissue. This person stated that there would be about a 10% chance that the anti-human antibodies would work on porcine tissues. The antibody companies mostly give the same "we did not try it on pig tissue" response. I understand that it is likely expensive to continue to test antibodies on a wide variety of animal tissues, so that is fine. But what do you guys think about the 10% chance? I would have said more than that. Thank you in advance for your time, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vibratome skipping sections
Hello, I have just started using a vibratome (Leica VT1000S) to cut sections of fish embryos after whole mount in situ hybridization, but I've encountered a problem with this. I have been setting the vibratome to cut sections of 50um to 70um in thickness (on the recommendation of a colleague who routinely uses this instrument), and I've noticed that after a few nice sections, the machine starts skipping a section (i.e. the blade will just pass over the block). When this happens, the next section to be cut is approximately twice as thick (i.e. if my section thickness is set to 70um, the blade will miss a section, and will then cut a section that is approxaimtely 140um thick). I'm not sure if this is a problem with the settings I'm using, or with the way I prepare the blocks I tend to cut with a speed of 5-7, and a frequency of ~8 (again, based on settings recommended by a colleague). To prepare the block, I embed the embryos in 30% gelatin in PBS, and once set, I trim it and fix the block in 4% PFA for 48hrs. I try not to make my blocks too tall, and they seem nice and firm when I mount them on the machine There are certainly a lot of variables that I could play with here - and I could probably spend weeks fiddling with it! I would really appreciate hearing from anyone else has experienced this, and who has ideas of how to fix it. Thank you very much, Andrew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue processing
Sheri Your processing cycle is too long. We process 20 minutes per station for mouse tissue only 2 absolutes and 2 xylenes and 3 paraffins. Even 3 absolutes and 3 xylenes at 20 minutes a station we find over processes the tissue. Liz From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sheri Kelemen Sent: Wed 3/17/2010 4:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue processing Hi everyone, I need some advice. I have been doing tissue processing (on an old Shandon Hypercenter), embedding and sectioning for 15 yrs. and never really had any problems. I recently processed mouse tissues on a brand new tissue processor (Leica ASP300S). When I went to cut 5 micron sections the tissues (spleen, heart, aorta and lung) were very dry and brittle. They made what looked like sawdust after each cut. Soaking them in cold ammonia water helped some; but they still did not cut nicely. Here is my protocol: I perfusion fixed the mouse with fresh 10% Neutral Buffered Formalin (NBF). Then I removed the tissues and cut them in smaller pieces (no more that 5mm thick) and put them in 10% NBF for 24 hrs., then washed with PBS and stored in 70% ethanol (made with PBS) for 2 days before processing (only because I didn't have time to process till then). My processing protocol is as follows: 70% ETOH x1 1hr. 95% ETOH x2 1hr each 100% ETOH x3 1hr each Xylene x3 1hr each wax x3 45 min each 60°C Can any one tell me why they think my tissues are so so dry. What could I change for the next time I do this? Thank you. Sheri Kelemen Research Associate Cardiovascular Research Center, Temple University 3500 N. Broad St. Philadelphia, PA 19140 215-707-3170 work skele...@temple.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue processing
Hi everyone, I need some advice. I have been doing tissue processing (on an old Shandon Hypercenter), embedding and sectioning for 15 yrs. and never really had any problems. I recently processed mouse tissues on a brand new tissue processor (Leica ASP300S). When I went to cut 5 micron sections the tissues (spleen, heart, aorta and lung) were very dry and brittle. They made what looked like sawdust after each cut. Soaking them in cold ammonia water helped some; but they still did not cut nicely. Here is my protocol: I perfusion fixed the mouse with fresh 10% Neutral Buffered Formalin (NBF). Then I removed the tissues and cut them in smaller pieces (no more that 5mm thick) and put them in 10% NBF for 24 hrs., then washed with PBS and stored in 70% ethanol (made with PBS) for 2 days before processing (only because I didn't have time to process till then). My processing protocol is as follows: 70% ETOH x1 1hr. 95% ETOH x2 1hr each 100% ETOH x3 1hr each Xylene x3 1hr each wax x3 45 min each 60°C Can any one tell me why they think my tissues are so so dry. What could I change for the next time I do this? Thank you. Sheri Kelemen Research Associate Cardiovascular Research Center, Temple University 3500 N. Broad St. Philadelphia, PA 19140 215-707-3170 work skele...@temple.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] technologist productivity
I apologize in advance if I offend anyone with this inevitably touchy question. In the last two years we have lost one "older" histotechnologist, and the routine reliable services of another of that group and are now facing the issue of what can be expected from their replacements. I have little feel for this other than my experience with these and other previous employees, so I am hoping that some of you would be willing to share your thoughts/data with me either in response on this listserv, or alternatively "off line" directly to my email address. I am interested in some numbers on how many blocks one could expect to have embedded within an hour, with break down for simple larger or single pieces vs multiple small bx's like GI bx's or prostate needle bx's. I am looking for similar information in regard to the number of sections one could expect to be cut in an hour from routine blocks (and for this purpose, small and large bxs should be included in the same analysis-there is so much variation in regard to the number of sections that labs routinely cut from "smalls", so just a general mix of the number of slides would be helpful.) Thank you in advance for sharing-we are just trying to maintain reasonable expectations from our new/replacement technologists. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] question about mouse perfusion
Hi Li, Try 1-2 ml/min perfusion rate. Yes 10ml/min is way too fast. I know it's tricky; I've done it by hand and by pump. Perfuse with PBS or Saline (same thing) first, then 4% PFA til mouse is stiff, then after taking the brain out immerse it in increasing grades of sucrose (10%-->20%-->30%) at 4C til brain sinks to the bottom of the vessel each time. Takes longer to sink with increasing grades - I think if I remember correctly in 10% it's only half an hour, 20% is a few hours, and 30% is overnight. Something like that. Some people skip the 20% and just do 10% and 30%. Some people just do 30%. Sucrose helps cryopreserve the delicate brain tissue. Regards, Merced --On Wednesday, March 17, 2010 11:58 AM -0700 Li Zhang wrote: Dear all, I have just started to learn how to do perfusion in mice and this site has helped me a lot. We don't have the pump or Perfusion one in the lab, and probably won't be able to get one in the near future, so I'm using the hand injection method. I'm using a 60 ml syringe with 21G needle. I am doing perfusion in order to do immunofluorescence on the brain. My question is: can anyone give me a rough idea of how fast I should inject ( like ml/min). I think I've tried like 30 ml in 3 min, and I suspect that it's too fast because I do observe tissue swelling sometimes. And another question, which of the following is better for perfsusion: 1) PBS and then 4% PFA 2) Saline and then 4% PFA 3) sucrose--> saline--> PFA? Thank you very much for your help! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD3 on mouse
Dako's rabbit anti-CD3 cross reacts with mouse Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mauger, Joanne Sent: Wednesday, March 17, 2010 1:31 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD3 on mouse Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD3 on mouse
Hi All, Does anyone know a CD3 antibody that works well on FFPE mouse tissue? Thanks in advance, Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura MicroArray Apparatus
We are making our own research / immunohistochemistry controls and have found that the tips, as well as the molds are becoming quite expensive. The tips ( especially in the hands of a resident) have a tendency to be rather "soft" and loose their shape rather quickly. Any other users having this issue? Anyone know of someone that provides this service for a fee? We would provide tissues, pay for block making. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] question about mouse perfusion
Dear all, I have just started to learn how to do perfusion in mice and this site has helped me a lot. We don't have the pump or Perfusion one in the lab, and probably won't be able to get one in the near future, so I'm using the hand injection method. I'm using a 60 ml syringe with 21G needle. I am doing perfusion in order to do immunofluorescence on the brain. My question is: can anyone give me a rough idea of how fast I should inject ( like ml/min). I think I've tried like 30 ml in 3 min, and I suspect that it's too fast because I do observe tissue swelling sometimes. And another question, which of the following is better for perfsusion: 1) PBS and then 4% PFA 2) Saline and then 4% PFA 3) sucrose--> saline--> PFA? Thank you very much for your help! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ammonium chloride in ihc
Hello all, We are trying a new antibody and the protocol from the company suggests a 50 mM ammonium chloride wash before blocking and adding the primary antibody. What is the purpose of this--antigen retrieval, autofluorescent quenching, something else? We were thinking about trying this step as our protocol does not yield any staining. Emily A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: CAP cancer protocols and checklists
Here, the pathologist or the transcriptionist inserts the table, which has been typed into Word and saved as an autocorrect or a Dragon template, into the report. We used them for years and have updated according to the new standards this year. Is anyone using the tables from the CAP website? I have asked how to get them into our reports but seems we need to use the autocorrect approach with our system. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, March 17, 2010 12:24 To: 'Carol Bryant'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP cancer protocols and checklists We have the Meditech Computer system and we have built a data section between Micro and Diagnosis namde Synoptic Tumor Protocol nd have canned texts available for all tumor types that pull in the required information needed. All the Pathologist's have to do is enter the information and hit enter and it goes to the next field needed to fill in. That way it is done consistently and within the CAP protocols. Dawn D. Schneider, HT(ASCP) Lead Histology Tech Howard Young Medical Center 240 Maple St. Woodruff, WI 54568 715-356-8174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Tuesday, March 16, 2010 4:20 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP cancer protocols and checklists I am interested in how you are implementing the CAP cancer protocols in your laboratory? Are you using synoptic reporting, dictating from the checklists, etc? Any information you can provide would be greatly appreciated. Thank you in advance for your comments and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit plastics and Exakt System
Hi all, I just joined this mailing list, and I wonder if there is a subgroup or another list for people who work often with the Exakt cutting and grinding system. I use mainly Technovit 7200 and work with a lot of bone samples with some kind of biomaterial. Recently I started working with stented arteries, and had GREAT help with some issues from Gayle Callis and Lori Garcia. Thank you again girls! So, if there is a list or a group for "those people", where is it, or what is the URL? If not, I propose that we form one so we can help each other with these interesting techniques. I am sure I am not the only one who has had more than one "scratching the head" moments with those! What do you think? I am not sure if you can reply to this directly to my e-mail or if it has to go to the histonet group. My gmail is clqsousa. Thanks! Carol Bain Technical Research Assistant Histopathology Services Laboratory Comparative Pathology Deartment - Purdue University. (765) 494 0581 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP cancer protocols and checklists
We have the Meditech Computer system and we have built a data section between Micro and Diagnosis namde Synoptic Tumor Protocol nd have canned texts available for all tumor types that pull in the required information needed. All the Pathologist's have to do is enter the information and hit enter and it goes to the next field needed to fill in. That way it is done consistently and within the CAP protocols. Dawn D. Schneider, HT(ASCP) Lead Histology Tech Howard Young Medical Center 240 Maple St. Woodruff, WI 54568 715-356-8174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Tuesday, March 16, 2010 4:20 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP cancer protocols and checklists I am interested in how you are implementing the CAP cancer protocols in your laboratory? Are you using synoptic reporting, dictating from the checklists, etc? Any information you can provide would be greatly appreciated. Thank you in advance for your comments and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HPS Stain
I think there is confusion regarding the original question posed. They were not asking about the HPS stain. Some hospitals use an eosin/saffron stain in their routine H&E. I had another lab recently asking about the formula to prepare this solution instead of eosin. Any advise? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Wednesday, March 17, 2010 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPS Stain Hi Sheila, HPS (Hematoxylin-Phloxine-Saffron) is the routine stain we use. There are a few institutions in Canada that also still use this stain; around Ottawa and a few other places in Ontario and Quebec. Perhaps one of these institutions sent slides to your pathologist for referral/consult? It is quite a nice trichrome stain, but somewhat more complex and expensive, uses more staining dishes, and it's sometimes tricky to get a proper phloxine/saffron balance. Due to the use of H&E being more widespread, our pathology residents usually ask for H&E slides for their files when preparing for exams. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HPS Stain
Hi Sheila, HPS (Hematoxylin-Phloxine-Saffron) is the routine stain we use. There are a few institutions in Canada that also still use this stain; around Ottawa and a few other places in Ontario and Quebec. Perhaps one of these institutions sent slides to your pathologist for referral/consult? It is quite a nice trichrome stain, but somewhat more complex and expensive, uses more staining dishes, and it's sometimes tricky to get a proper phloxine/saffron balance. Due to the use of H&E being more widespread, our pathology residents usually ask for H&E slides for their files when preparing for exams. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Unsubscribing adventures
Several people have expressed frustration with the unsubscribe process. The process will not work unless you unsubscribe the same Email address that you used when you signed up for the list. If you have changed Email addresses, and the old address is forwarding to the new address, you will receive the mail, but when you unsubscribe under the new address, the listserv bot doesn't recognize it, and so nothing happens. Return with us now to those thrilling days of yesteryear, when you signed up for the list... What was your Email address back then? I hope this helps. Eric Hoy === Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric@utsouthwestern.edu === > Date: Tue, 16 Mar 2010 10:01:22 -0700 > From: "Vanessa Avalos" > Subject: [Histonet] UNSUBSCRIBE > To: "'HISTONET LISTS'" > Message-ID: <001001cac52a$4ef4ba00$ecde2e...@com> > Content-Type: text/plain; charset="UTF-8" > > I can only speak for myself but yes, I am aware of all steps to be taken in > unsubscribing. Have followed them more than once but have been unsuccessful. > So yes, my frustration as lead me to write IN ALL CAPS! :) > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: proficiency testing for Muscle EHC- cont'd.
Carol, I take these kinds of CAP issues to mean, develop your own program for proficiency testing and show how you have validated it, then show it to CAP, they usually want to know that you have a plan in place even if it is something you developed yourself. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Tuesday, March 16, 2010 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: proficiency testing for Muscle EHC- cont'd. Histonetter's I only received one reply on my question regarding "what everyone is doing for proficiency testing of enzyme HC staining for muscle"...espeically for CLIA.compliance, now that I will lose my indepent reviewer. My thanks to Jan Minchew for her reply. I am working from that for now, though my sitiation somewhat different. .but, I am finding it particularly frustrating finding an answer to this question over the last three months or more, that I have searched everywhere from the histonet to the internet toevery friend I have doing EHC. Is it required under CLIA? My auditor says yes..so I am trying to comply, though I cannot fiind it specifically in any CLIA reg. Who is the CLIA expert out there...and why is this not included in HQIP? Can someone shed more light. Thx. CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: [Histonet] Spencer AO 820 microtome questions
Scott, You can look for the parts with some of the used equipment vendors, that "black beauty" was my favorite microtome of all times, it was heavy enough to cut plastic sections on when the new microtomes weren't, I am sure it would serve you well if you find a knife holder (you can purchase disposable blade holders that fit right in where the permanent blades went) and block holder. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Parker Sent: Tuesday, March 16, 2010 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Spencer AO 820 microtome questions Dear all, I have access to a Spencer AO 820 microtome (a "black beauty" in Histo-parlance) that is does not have a disposable blade holder nor a chuck for holding paraffin cassettes. The microtome otherwise appears to be in good condition. I would like to get suggestions for where I might be able to purchase a chuck and disposable blade holder for this classic model. Alternatively, would it be a better idea for me to put this piece of equipment on display in a glass case and instead purchase a newer microtome. My work involves relatively low volume, basic sectioning of paraffin blocks for teaching and research. I don't need anything too fancy, just a microtome that is reliable and appropriate for student researchers to use. Thank you in advance for your responses. Scott Scott L. Parker, Ph.D. Assistant Professor Biology Coastal Carolina University P.O. Box 261954 Conway, SC 29528-6054 Office Phone: (843)-349-2491 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mc Coy fixative
Hi Histonetters I am having a senior moment. Can anyone tell me what McCoy fixative is made of and what it is used for? Thanks in advance for the help. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Accessioning Similar Samples
We have agar based markers - red, blue, green, yellow, orange, black, that we include in the block for similar tissues - as we have several that be separated. It is noted in the gross and confirmed at micro that the markers are present. After we've gone through the colors once, we put two, three, etc. It has worked well for us. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Accessioning Similar Samples
Sara, Unfortunately the response you got is more common than I would like to see. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Accessioning Similar Samples
Regarding the need to avoid accessioning similar samples consecutively, I addressed this last week with my bosses. Specifically, I asked that spleen specimens not be accessioned consecutively (because of shattering and static and the possibility of fragments being carried over); my response was that I should just not CUT the blocks consecutively. Not the point I was trying to make but typical in some cases when addressing the issues histotechs face, true? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
