Re: [Histonet] cassette labels erased by processor

[Malika Benatti]

Another option would be to invest in a cassette labeling machine, they  
are few a available in the market, we use the Surgipath Cassette  
Marker, they are pretty good as the ink fix with formalin and survive  
processing.
Though ribbon need to keep refrigirated when not in use(overnight and  
weekend) and printing heard need to be clean at least once a week, but  
apart from tha it is pretty straight forward to use,
also it can be program so it make printing a large number of cassette  
painless .


I have also used in tha past cassette marker that use thermal print  
ribbon so again no risk of lab number not surviving processing.So if  
you lab as some spare cash to spend  get few on trial in, az they are  
worth it.


Best wishes,

Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London

 " ... Smile it confuses people ..."

On 1 Apr 2010, at 00:24, Amos Brooks  wrote:


Indeed,
 One of the most EVIL products ever designed is the "Permanent  
Lab Marker" by VWR. Talk about false advertising! We have  
researchers drop off buckets of cassettes that they have labeled  
using these wonderful products. Pencil is the ONLY acceptable means  
of labeling cassettes. It is just not worth the risk to use anything  
else. (Except cassette labelers they are specifically designed for  
this and have a sales rep you can beat up if they fail.) Even pens  
specifically designed for cassettes have failed at times.


Just my $0.02,
Amos


Message: 23
Date: Wed, 31 Mar 2010 15:40:44 +0100
From: Malika Benatti 
Subject: Re: [Histonet] cassette labels erased by processor
To: Catherine Breen 
Cc: Histonet List 
Message-ID: 
Content-Type: text/plain;   charset=us-ascii;
format=flowed;  delsp=yes


Hi there,
I would suggest to use a pencil rather than a marker pen to label
cassettes when processing cassette with the Sakura VIP , my lab had a
number of the so called solvent proof pen on trial and have yet to
find one that survive processing.

Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London

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Re: [Histonet] cassette labels erased by processor

[Amos Brooks]

Indeed,
 One of the most EVIL products ever designed is the "Permanent Lab
Marker" by VWR. Talk about false advertising! We have researchers drop off
buckets of cassettes that they have labeled using these wonderful products.
Pencil is the ONLY acceptable means of labeling cassettes. It is just not
worth the risk to use anything else. (Except cassette labelers they are
specifically designed for this and have a sales rep you can beat up if they
fail.) Even pens specifically designed for cassettes have failed at times.

Just my $0.02,
Amos


Message: 23
Date: Wed, 31 Mar 2010 15:40:44 +0100
From: Malika Benatti 
Subject: Re: [Histonet] cassette labels erased by processor
To: Catherine Breen 
Cc: Histonet List 
Message-ID: 
Content-Type: text/plain;   charset=us-ascii;   format=flowed;
 delsp=yes

Hi there,
I would suggest to use a pencil rather than a marker pen to label
cassettes when processing cassette with the Sakura VIP , my lab had a
number of the so called solvent proof pen on trial and have yet to
find one that survive processing.

Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London
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RE: [Histonet] mouse perfusion rate

[Andrea T. Hooper]

Very interesting! Coming out the nose is definitely bad for any work I have 
done in the past - lungs get blown out, liver doesn't perfuse well and bone 
marrow looks horrific. However, if you are working with PFA and doing a 
post-fix anyway, you will probably be fine. If you are using GA and counting on 
the perfusion to ensure excellent fixation for things such as lacZ staining 
(b/c post-fix in GA never works well for bone or deep into tissues) then 
blowing it out the nose is bad. Very bad.

Andrea 
 

--- On Mon, 3/29/10, charles.scou...@leica-microsystems.com 
 wrote:


From: charles.scou...@leica-microsystems.com 

Subject: RE: [Histonet] mouse perfusion rate
To: lei...@buffalo.edu, saby_josep...@yahoo.com, mak...@ufl.edu, 
histonet@lists.utsouthwestern.edu
Date: Monday, March 29, 2010, 6:26 PM


I have perfused mice and rats at 300 mm Hg, about double physiological
level, don't know what that made the flow rate.  All mammals have the
same blood pressure (within tolerances), so it is easier to select a
suitable pressure to use than a flow rate, which varies dramatically.  



I look at brain, never pay any attention to the gut.  Clear fluid comes
out the nose, that is a good sign.  There are pressure release valves
across the cribiform plate to release CSF if there is too much.  I am
flooding the system, fluid coming out the nose means the extracellular
fluid and CSF is being replaced as well as vascular blood.  Good.  



The tissue is quality is excellent, free of red blood cells, can be
unshrunk depending on the tonicity (should be sub isotonic) of the
fixative fluid.  Have looked at Nissl and EM material, no evidence of
damage to the tissue.



If gut is extended, might have something to do with the large intestines
job of removing fluid from feces, and flooding the system swells the
tissue.  But does it matter?  Do you use that tissue?  What is the
tissue quality if you use it after physiological pressure perfusion.



Cordially,

Charles W. Scouten, Ph.D

Product Manager, MNL

Biosystems Division



Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America

Telephone 630 964 0501

facsimile +1 630 964 0576

www.MyNeuroLab.com  

www.leica-microsystems.com  



IMPORTANT - This email and any attachments may be confidential. Any
retransmissions, dissemination or other use of

these materials by persons or entities other than the intended recipient
is prohibited. If received in error, please contact

us and delete all copies. Before opening or using attachments, check
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to resupplying any affected attachments. [Any representations or
opinions expressed in this email are those of the

individual sender].





From: Merced M Leiker  [mailto:Merced M Leiker
] 
Sent: Monday, March 29, 2010 9:05 AM
To: Joseph Saby ;
charles.scou...@leica-microsystems.com; mak...@ufl.edu;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mouse perfusion rate



Hi Joe, 

Thanks for that notice about flow rates. But I think for the mouse you 
meant 1-3mls/min (not per 10min?)... 

Regards, 
Merced 

--On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby 
< saby_josep...@yahoo.com> wrote: 

> 
> 
> All- 
> 
> From previous work with rat perfusions, the flow rate was about 10 
> ml/minute. If I had to guess, the equivalent flow rate for a mouse
would 
> be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will 
> definitely cause blowout artefacts. 
> 
> Joe Saby, BA HT 
> 
> 
> 
> 
> __ 
> From: Merced M Leiker < lei...@buffalo.edu> 
> To: charles.scou...@leica-microsystems.com; mak...@ufl.edu; 
> histonet@lists.utsouthwestern.edu 
> Sent: Fri, March 19, 2010 9:21:38 AM 
> Subject: RE: [Histonet] mouse perfusion rate 
> 
> The vasculature will leak too much and the mouse will get bloated - 
> you'll 
> see it first in either the intestines blowing up like a balloon or
fluid 
> coming out of the nose. Just not the same as the heart pumping when
the 
> mouse is alive with intact physiology and normal functioning. Don't
know 
> exactly why, but that's what happens when you go too fast. Perhaps the
> vasculature has lost its control to compensate for the pressure? I'm
not 
> a 
> physiologist so I'm not sure why...maybe someone on the Histonet can 
> answer 
> that? 
> 
> Regards, 
> Merced 
> 
> --On Thursday, March 18, 2010 5:49 PM -0500 
> charles.scou...@leica-microsystems.com wrote: 
> 
>> 
>> 
>> Why not? What happens? One would think the mammalian cardiovascular 
>> system could withstand physiological pressures and flow rates, at
least 
>> for one lifetime? 
>> 
>> 
>> 
>> 
>> Cordially, 
>> 
>> Charles W. Scouten, Ph.D 
>> 
>> Product Manager, MNL 
>> 
>> Biosystems Division 
>> 
>> 
>> 
>> Leica Biosystems Richmond, Inc. 
>> 5205 Route 12 
>> P.O. Box 528 
>> Richmond, IL 60071 
>> United S

[Histonet] IHC detection for Pig tissue?

[Reuel Cornelia]

I just wanted to know if anybody who are working with Pig bone tissue fix in 
10%NBF and decalcified in 14% EDTA, citrate buffer (dako) antigen retrieval  
pH7.0 and ph 9.0 by steaming for 20 minutes, what is their IHC detection kit 
for mouse monoclonal and rabbit polyclonal antibodies. I am using a Powervision 
kit and I have a lot of background staining even with my negative staining. 
Please help.



Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768



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[Histonet] Just three months?????

[Jeffrey Silverman]

CAP is now saying no more gross processing of small things that are  entirely 
submitted- it's all gross examination now whether we mean straining  
currettings into a cassette or dissecting a complex cancer resection. Dumb as 
all get out if you ask me. 

As for the three month training thing, Joe, I'm not so sure about that. They 
seem to spell out specific amounts of college education required IN ADDITION to 
training in the laboratory. 
The requirement first spells out  two education choices-
 1: an earned associates degree in medical laboratory  science 

OR 2:Education/training equivalent to the above that includes at least 60 
semester
 hours or equivalent from an accredited institution. This education 
MUST (caps mine)  include 24 semester hours of medical laboratory technology 
courses, OR 24
 semester hours of science courses that includes 6 semester hours of 
chemistry,
 6 semester hours of biology, and 12 semester hours of chemistry, 
biology
 or medical laboratory technology in any combination.

So in addition to all the college courses, which we all know you need to count 
and measure six endoscopic biopsies and put them in a cassette, much more 
training and experience needed than what an unregistered on the job tech needs 
to orient and embed them properly LOL!!!    CLIA then requires  that the
 individual must have (additional)  laboratory training including either 
completion of 
 a clinical laboratory training program approved or accredited by 
the ABHES,  NAACLA, or other organization approved by HHS (note that 
this training may  be included in the 60 semester hours listed 
above), 
OR at least 3 months  documented laboratory training in each 
specialty in which the individual performs high complexity testing. 

Now there are grandfathering clauses in CLIA which may enable folks (like 
myself) to continue to gross. I haven't digested  that yet, but I'm ready to 
get that job in Pathmark if necessary. 

Sheesh, does it ever end? 

Jeff Silverman
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FW: [Histonet] Coverslipping with SubX

[Vanessa Avalos]

Sally, 

I added SubX to my acrymount since I have plenty of that in stock. It
worked!!  And I am so glad I can use up what I have instead of having to
purchase a case of Sub X glue. My slides look clear and film free. I now am
more confident in passing these slides over to be read. 

Thanks for all the helpful suggestions everyone!

-Original Message-
From: Breeden, Sara [mailto:sbree...@nmda.nmsu.edu] 
Sent: Tuesday, March 30, 2010 11:25 AM
To: Vanessa Avalos
Subject: RE: [Histonet] Coverslipping with SubX

Try thinning out the Sub-X-based mounting medium with some Sub-X.  It
sounds as if your mounting medium is too thick.  Try that and see if it
helps.  You want your mounting medium to drip like cheap pancake syrup
would drip - not too thick and not too thin.  Too thick makes bubbles.

I use Sub-X on my processor but use xylene on my autostainer, but that's
because I have an tape coverslipper.  I know you mean you're
coverslipping by hand but see if the thinner medium helps you.  Let me
know!

Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM  87106
505-841-2576


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[Histonet] ***NEW HISTOLOGY JOBS***

[K.C. Carpenter]

Are you interested in hearing about new job opportunities? I am a one of the 
founders of a Healthcare Recruiting firm that specializes in placing Lab 
Professionals. We work exclusively on permanent positions and have clients 
across the country. We are completely free of charge to candidates and are 
currently working on numerous Histology positions. Our clients often assist 
with relocation expenses. 


 


Below is a list of some of the Histology opportunities we are currently working 
on.




 




New York, NY - Histotech 3rd shift


Las Vegas, NV - Histotech 3rd shift


Las Vegas, NV - Histology Supervisor - 3rd shift


Palm Springs, CA - Histotech - 1st shift


Los Angeles, CA -  Histotech


Northeastern, VA - Histotech 2nd shift


Southern, NH - HTL and HT


Atlanta, GA - Atlanta - Histotech


Atlanta, GA - Atlanta - Pathology Coordinator (HT or CT)


Southeastern, MA - IHC Tech


Southeastern, MA - Histotech



 






If you're interested in learning more about any of these opportunities then 
please email me a resume and let me know how best to get in touch with you.  
If none of these are a fit please let me know what you'd be interested in and 
where you're looking so I can tailor a search for you.  With the New Year upon 
us many of our clients have fresh hiring budgets and will be looking to add 
people over the next several months. We work on positions at all levels and 
cover the entire US. To view some additional opportunities please visit our 
website at www.ka-recruiting.com
.  






Sincerely














KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



k...@ka-recruiting.com



www.ka-recruiting.com
  






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RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines

[Mahoney,Janice A]

But above that after the education piece it says "in Addition".
Jan, Omaha

-Original Message-
From: Joe Nocito [mailto:jnoc...@satx.rr.com]
Sent: Wednesday, March 31, 2010 4:08 PM
To: Mahoney,Janice A; Histonet
Subject: Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines

just had a lively discussion at work. My take is that the only thing CAP
changed was that they combined the "processing" and "grossing" pieces
together again, which I don't know why they split them in the first place.
But you don't have the entire CAP note and many people miss this.  The last
item states OR three months of documented laboratory training in the high
complexity area. Again, my take is that an unregistered histotech can have
at least three months of documented training in grossing complex specimens,
have the record signed off by the medical director and be ok. How far off am
I?

Joe
- Original Message -
From: "Mahoney,Janice A" 
To: "Histonet" 
Sent: Wednesday, March 31, 2010 2:44 PM
Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines


Is anyone concerned about the new (old) grossing personnel guidelines from
CAP.  Many labs use people to "process " tissue.  No more!


ANP.11610 Phase II

If individuals other than a pathologist or pathology resident assist in
gross examinations, do such individuals qualify as high complexity testing
personnel under CLIA regulations?

NOTE: The laboratory director may delegate the dissection of specimens to
non-pathologist individuals; these individuals must be qualified as high
complexity testing personnel under CLIA regulations. The minimum
training/experience required of such personnel is:

 1.  An earned associate degree in a laboratory science or medical
laboratory technology, obtained from an accredited institution, OR
 2.  Education/training equivalent to the above that includes at least 60
semester hours or equivalent from an accredited institution. This education
must include 24 semester hours of medical laboratory technology courses, OR
24 semester hours of science courses that includes 6 semester hours of
chemistry, 6 semester hours of biology, and 12 semester hours of chemistry,
biology or medical laboratory technology in any combination. In addition,
the individual must have laboratory training including either completion of
a clinical laboratory training program approved or accredited by the ABHES,
NAACLA, or other organization approved by HHS (note that this training may
be included in the 60 semester hours listed above), OR at least 3 months
documented laboratory training in each specialty in which the individual
performs high complexity testing.

The CLIA regulations on high complexity testing personnel may be found at HC
Testing Personnel.

In addition, the CLIA regulations include exceptions for grandfathered
individuals; these regulations (42CFR493.1489 and 1491) may be found at the
above Web address and at Grandfathered
Exceptions.

It is the responsibility of the laboratory director to determine whether an
individual's education, training and experience satisfies the requirements
of this checklist question.
Jan Mahoney
Omaha, NE


Sponsored by Catholic Health Initiatives and Immanuel Health Systems,
Alegent Health is faithful to the healing ministry of Jesus Christ,
providing high quality care for the body, mind and spirit of every person.

The information contained in this communication, including attachments, is
confidential and private and intended only for the use of the addressees.
Unauthorized use, disclosure, distribution or copying is strictly prohibited
and may be unlawful. If you received this communication in error, please
inform us of the erroneous delivery by return e-mail message from your
computer. Additionally, although all attachments have been scanned at the
source for viruses, the recipient should check any attachments for the
presence of viruses before opening. Alegent Health accepts no liability for
any damage caused by any virus transmitted by this e-mail. Thank you for
your cooperation.
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Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent 
Health is faithful to the healing ministry of Jesus Christ, providing high 
quality care for the body, mind and spirit of every person.

The information contained in this communication, including attachments, is 
confidential and private and intended only for the use of the addressees.  
Unauthorized use, disclosure, distribution or copying is strictly prohibited 
and may be unlawful.  If you received this communication in error, please 
inform us of the erroneous delivery by return e-mail message from your 
computer.  Additionally, although all attachments have 

Re: [?? Probable Spam] [Histonet] New CAP grossing guidelines

[Joe Nocito]

just had a lively discussion at work. My take is that the only thing CAP 
changed was that they combined the "processing" and "grossing" pieces 
together again, which I don't know why they split them in the first place. 
But you don't have the entire CAP note and many people miss this.  The last 
item states OR three months of documented laboratory training in the high 
complexity area. Again, my take is that an unregistered histotech can have 
at least three months of documented training in grossing complex specimens, 
have the record signed off by the medical director and be ok. How far off am 
I?


Joe
- Original Message - 
From: "Mahoney,Janice A" 

To: "Histonet" 
Sent: Wednesday, March 31, 2010 2:44 PM
Subject: [?? Probable Spam] [Histonet] New CAP grossing guidelines


Is anyone concerned about the new (old) grossing personnel guidelines from 
CAP.  Many labs use people to "process " tissue.  No more!



ANP.11610 Phase II

If individuals other than a pathologist or pathology resident assist in 
gross examinations, do such individuals qualify as high complexity testing 
personnel under CLIA regulations?


NOTE: The laboratory director may delegate the dissection of specimens to 
non-pathologist individuals; these individuals must be qualified as high 
complexity testing personnel under CLIA regulations. The minimum 
training/experience required of such personnel is:


1.  An earned associate degree in a laboratory science or medical 
laboratory technology, obtained from an accredited institution, OR
2.  Education/training equivalent to the above that includes at least 60 
semester hours or equivalent from an accredited institution. This education 
must include 24 semester hours of medical laboratory technology courses, OR 
24 semester hours of science courses that includes 6 semester hours of 
chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, 
biology or medical laboratory technology in any combination. In addition, 
the individual must have laboratory training including either completion of 
a clinical laboratory training program approved or accredited by the ABHES, 
NAACLA, or other organization approved by HHS (note that this training may 
be included in the 60 semester hours listed above), OR at least 3 months 
documented laboratory training in each specialty in which the individual 
performs high complexity testing.


The CLIA regulations on high complexity testing personnel may be found at HC 
Testing Personnel.


In addition, the CLIA regulations include exceptions for grandfathered 
individuals; these regulations (42CFR493.1489 and 1491) may be found at the 
above Web address and at Grandfathered 
Exceptions.


It is the responsibility of the laboratory director to determine whether an 
individual's education, training and experience satisfies the requirements 
of this checklist question.

Jan Mahoney
Omaha, NE


Sponsored by Catholic Health Initiatives and Immanuel Health Systems, 
Alegent Health is faithful to the healing ministry of Jesus Christ, 
providing high quality care for the body, mind and spirit of every person.


The information contained in this communication, including attachments, is 
confidential and private and intended only for the use of the addressees. 
Unauthorized use, disclosure, distribution or copying is strictly prohibited 
and may be unlawful. If you received this communication in error, please 
inform us of the erroneous delivery by return e-mail message from your 
computer. Additionally, although all attachments have been scanned at the 
source for viruses, the recipient should check any attachments for the 
presence of viruses before opening. Alegent Health accepts no liability for 
any damage caused by any virus transmitted by this e-mail. Thank you for 
your cooperation.

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[Histonet] New CAP grossing guidelines

[Mahoney,Janice A]

Is anyone concerned about the new (old) grossing personnel guidelines from CAP. 
 Many labs use people to "process " tissue.  No more!


ANP.11610 Phase II

If individuals other than a pathologist or pathology resident assist in gross 
examinations, do such individuals qualify as high complexity testing personnel 
under CLIA regulations?

NOTE: The laboratory director may delegate the dissection of specimens to 
non-pathologist individuals; these individuals must be qualified as high 
complexity testing personnel under CLIA regulations. The minimum 
training/experience required of such personnel is:

 1.  An earned associate degree in a laboratory science or medical laboratory 
technology, obtained from an accredited institution, OR
 2.  Education/training equivalent to the above that includes at least 60 
semester hours or equivalent from an accredited institution. This education 
must include 24 semester hours of medical laboratory technology courses, OR 24 
semester hours of science courses that includes 6 semester hours of chemistry, 
6 semester hours of biology, and 12 semester hours of chemistry, biology or 
medical laboratory technology in any combination. In addition, the individual 
must have laboratory training including either completion of a clinical 
laboratory training program approved or accredited by the ABHES, NAACLA, or 
other organization approved by HHS (note that this training may be included in 
the 60 semester hours listed above), OR at least 3 months documented laboratory 
training in each specialty in which the individual performs high complexity 
testing.

The CLIA regulations on high complexity testing personnel may be found at HC 
Testing Personnel.

In addition, the CLIA regulations include exceptions for grandfathered 
individuals; these regulations (42CFR493.1489 and 1491) may be found at the 
above Web address and at Grandfathered 
Exceptions.

It is the responsibility of the laboratory director to determine whether an 
individual's education, training and experience satisfies the requirements of 
this checklist question.
Jan Mahoney
Omaha, NE


Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent 
Health is faithful to the healing ministry of Jesus Christ, providing high 
quality care for the body, mind and spirit of every person.

The information contained in this communication, including attachments, is 
confidential and private and intended only for the use of the addressees. 
Unauthorized use, disclosure, distribution or copying is strictly prohibited 
and may be unlawful. If you received this communication in error, please inform 
us of the erroneous delivery by return e-mail message from your computer. 
Additionally, although all attachments have been scanned at the source for 
viruses, the recipient should check any attachments for the presence of viruses 
before opening. Alegent Health accepts no liability for any damage caused by 
any virus transmitted by this e-mail. Thank you for your cooperation.
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[Histonet] DAKO Her2 antibody

[Bernice Frederick]

Anyone out there noticing problems with the Her2 antibody for Dako? Seems
real strong and deteriorating quickly. We order it in lots, so you can
imagine... Seems like every run is different. Same stainer, same titre ,
same tech. Tissue is from all over the country and world so we cannot
control fixation etc.

Bernice

 

 

Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

ECOGPCO-RL 

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723

 

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[Histonet] RE: haunted Cryostat

[Reynolds,Donna M]

We had the same problem with our Leica 3050S. It happened occasionally then 
became more and more frequent. We went through a lot of the same hoops you have 
gone through. When it shut down one day while I was cutting I knew it had to be 
a machine malfunction. Leica replaced something that was connected with the arm 
that holds the block and then goes to the power. This was about 13 months ago 
and I am afraid it is starting it again. I come in and the computer panel says 
"Power Failure" but it is still cooling. I think this is how it started out 
last time. In the beginning I figured we had had an electrical outage or 
someone was messing with it at night.  

--

Message: 3
Date: Mon, 29 Mar 2010 15:20:35 -0400
From: mtitf...@aol.com
Subject: [Histonet] Is my Leica CM 1850 cryostat haunted?
To: histo...@pathology.swmed.edu
Message-ID: <8cc9d8c0aa7a02f-b40-5...@webmail-m081.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"



We have a Leica CM 1850 cryostat that about once every three weeks to a month, 
turnes itself off! Has anyone else experienced this? How do I fix it?

Initially, we thought someone after hours was turning it off, maybe someone in 
housekeeping or a passing med tech. That was not the case.
Later our Biomedical people replaced a capacitor or something in the bowels of 
the cryostat thinking that may be faulty and contribute to the problem. That 
did not help either.
Still later, the cryostat was put on its own circuit (No help), and then after 
that, we tried an uninterruptible power source thinking minor power 
fluctuations were the cause (no help either).

Anyone know how to fix this problem?. It is disconcerting to walk into the lab 
in the morning and the cryostat is at room temperature and the OR is going to 
have a busy day.

Michael Titford
Pathology USA
Mobile AL






--
*

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Re: [Histonet] GMS stain

[Malika Benatti]

Hi Margaret,

What problem are you experiencing ?
Are you over impregnated or under impregnated ?
At what thickness do you cut your Section ? Ideally 3 to 4 microns
How do you make up you Hexamine solution ? if you get contamination of
Hexamine solution make sure that glass ware is clean.

Try this.
Make 0.75 g of hexamine in 100 ml coplin jar / leave it dissolve in
distilled H2O at 60 oC while your slides are in chromic acid. When ready
slowly ad 18 drops (approximately 1ml) of 5% silver nitrate, (make sure
solution remain clear if cloudy, then glassware is contaminated and repeat
this steps with chromic acid clean glassware) then add 2 ml of borax. Sliver
solution should remain clear. add slides and check macroscopically slide
after 5 to 7 mins, then at 5 mins interval after that. Depending on tissue,
it should not take more that 15 mins to develop, then carry to next step.

Hope this help

Best wishes

Malika


Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Hospital
London, UK


On Wed, Mar 31, 2010 at 7:46 PM, Perry, Margaret  wrote:

> We sometimes have problems with the stain if we use positive slides.
> Margaret
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
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[Histonet] Haunted Cryostat

[SHargrove]

We had the exact same problem that just started out of no where. One night
I came in  really late to do a post and went through a department that
backed up to ours. Floor care was in there stripping the floors. The
cryostat was off again when I went in the room. The next day we used that
plug for our heat gun and flipped the cryostat off. They did  not always
use that plug, just had started after a desk had been moved.  When they
tripped the breaker they would reset it , but our machine had to be turned
back on every time. We had a new plug installed that day. It would be nice
if it was that simple for you.

(Embedded image moved to file: pic05610.jpg)___
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[Histonet] Re: retention of prosthetic valves

[Robert Richmond]

Anne Marie (where? Perhaps in Canada, which is going to have different
rules from the USA) >>was wondering what your hospital/centre's
pathology policies indicate in terms of the time you retain prosthetic
valve specimens? Months? Years?<<

I don't know of any specific policies. I've had a Carbomedics aortic
valve prosthesis now for 8 years. In 2005 a colleague of mine did an
autopsy on a man who had one (and died of unrelated causes), and I
took the opportunity to call Carbomedics to ask them if they wanted
the valve, and if they'd want mine when the time comes.

I was pleased to find out that they don't want them back, because
they're working just fine.

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] MM24 mounting media

[Houston, Ronald]

We use it both for manual and automated coverslipping with great results

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
tigger...@aol.com
Sent: Wednesday, March 31, 2010 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MM24 mounting media


Hello,

Does anyone use MM24 mounting media from surgipath?  We use cytoseal 60
but I can get the MM24 at a lower price.  If anyone has used this, could
you tell me whether you were satisfied with the product or not?  Thanks
so much.

Brandi Higgins
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[Histonet] GMS stain

[Perry, Margaret]

We sometimes have problems with the stain if we use positive slides.
Margaret
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[Histonet] RE: Histonet Digest, Vol 76, Issue 45

[John O'Brien]

Denise, Note#16 is a guy Named Felton Nails , I have done business with
him in the past he at Children hospital in Texas, try  calling him and
see if you would try our paraffin,  You can mention my name and let him
know I said hello
John

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: None
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 45


Send Histonet mailing list submissions to
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To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."


Today's Topics:

   1. Coverslipping with SubX (Vanessa Avalos)
   2. Haunted cryostat/Thanks! (mtitf...@aol.com)
   3. GMS on decal tissue (Morken, Tim)
   4. questions (Webb, Dorothy L)
   5. Re: her2 validation (Pat Laurie)
   6. FREE MONEY! (Jackie M O'Connor)
   7. RE: questions (Garrison, Becky)
   8. Thermo's Excelsior Tissue Processor Program's
  (Akemi Allison-Tacha)
   9. re: cyto prep tech (Kim Tournear)
  10. her-2 neu validation (Debbie Nannenga)
  11. RE: questions (Tony Henwood)
  12. RE: RE: Leica Paraplast (connie grubaugh)
  13. (no subject) (Green JumpyOne)
  14. Fwd: [Histonet] (no subject) (Malika Benatti)
  15. RE: Thermo's Excelsior Tissue Processor Program's
  (Heckford, Karen - SMMC-SF)
  16. RE: RE: Leica Paraplast (Nails, Felton)
  17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett)
  18. Texas Society for Histotechnology April 23-25, 2010
  (kdwyer3...@aol.com)
  19. cassette labels erased by processor (Catherine Breen)
  20. RE:  her2 validation (Weems, Joyce)
  21. RE: cassette labels erased by processor (Sherwood, Margaret )
  22. FW: [Histonet] cassette labels erased by processor (Cheri Miller)
  23. Re: cassette labels erased by processor (Malika Benatti)


--

Message: 1
Date: Tue, 30 Mar 2010 11:15:38 -0700
From: "Vanessa Avalos" 
Subject: [Histonet] Coverslipping with SubX
To: "'HISTONET LISTS'" 
Message-ID: <01cad035$00d2f5b0$0278e1...@com>
Content-Type: text/plain;   charset="us-ascii"

Is anyone using SubX ? I am trying it out and am having some difficulty
coverslipping. I am using the Subx glue as directed since Acrymount
didn't seem to work as well. I still get a hazy film under the glass and
am getting big air bubbles as well. I can eventually get them out but
its just takes a while and you know how time is precious when you have a
line of slides to stain. The process is not as smooth as before. I
really would like this to work out for me and eliminate xylene.

Any suggestions??

 

Vanessa

Fax: 602-277-2134

 



--

Message: 2
Date: Tue, 30 Mar 2010 14:44:26 -0400
From: mtitf...@aol.com
Subject: [Histonet] Haunted cryostat/Thanks!
To: histo...@pathology.swmed.edu
Message-ID: <8cc9e5028adfb36-1500-2...@webmail-m010.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"



Thank you everyone who responded to my problem with a Leica CM 1850
cryostat turning itself off. About 11 people responded with tips. Thank
you!! The Histonet is great!! In answer to some enquirys:
1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts
for an hour. Jackie O' Conner asks if it is set to go back "on" after
the defrost. I have no idea. It defrosts well the rest of the time, and
turns itself back on. Brian Cornett-Early recommends changing the start
device on the compressor.
2) Someone else asked if power is getting to the cryostat -  Yes, when
it turns itself off, the on/off switch is on "off". All you have to do
is flip the switch and it starts pumping and  cooling.
3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on
the side of the compressor. That is probably where we will start. Mari
Ann works for Leica so it sounds like good advice.

Thank you everyone. Since it only breaks down about once a month, it
will take a long time to determine if the problem is fixed.

Michael Titford
Pathology USA
Mobile AL USA



--

Message: 3
Date: Tue, 30 Mar 2010 11:52:17 -0700
From: "Morken, Tim" 
Subject: [Histonet] GMS on decal tissue
To: histonet 
Message-ID:

<1aaf670737f193429070841c6b2add4c013b16c...@exmbmcb15.ucsfmedicalcenter.
org>

Content-Type: text/plain; charset=us-ascii

Has anyone seen or heard of problems with Grocott Methenamine Silver
staining for fungi on decal tissues? Specifically, background or false
positives? I can't find anything in 

RE: [Histonet] MM24 mounting media

[Roberta Horner]

I use this and have had no problem with it.
Roberta

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
tigger...@aol.com
Sent: Wednesday, March 31, 2010 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MM24 mounting media


Hello,

Does anyone use MM24 mounting media from surgipath?  We use cytoseal 60 but I 
can get the MM24 at a lower price.  If anyone has used this, could you tell me 
whether you were satisfied with the product or not?  Thanks so much.

Brandi Higgins
___
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[Histonet] MM24 mounting media

[tigger13b]

Hello,

Does anyone use MM24 mounting media from surgipath?  We use cytoseal 60 but I 
can get the MM24 at a lower price.  If anyone has used this, could you tell me 
whether you were satisfied with the product or not?  Thanks so much.

Brandi Higgins
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RE: [Histonet] retention of prosthetic valves

[Weems, Joyce]

We photograph and retain only if requested. If not requested, we discard with 
the regular specimens usually at least a month. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lise Matzke
Sent: Wednesday, March 31, 2010 12:22
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] retention of prosthetic valves

Hi there,
 
I was wondering what your hospital/centre's pathology policies indicate in 
terms of the time you retain prosthetic valve specimens?  Months?  Years?
 
 
thanks for your feedback,
 
Anne Marie

***CONFIDENTIALITY NOTICE***
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the addressee and may contain information that is privileged and confidential.  
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unauthorized individuals is strictly prohibited. If you have received this 
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and delete the original and all copies from your system.

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It may contain information that is privileged and 
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[Histonet] Message-ID:

[Andrew Burgeson]

I use nothing but SurgiPath (now LEICA) Blue Ribbon
Paraffin.

I run dermpath labs and this stuff is formulated for optimal
skin sectioning.



Highly recommend ithavent used anything else for 13
years.

AB

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Re: [Histonet] RE: lab markers KP

[Merced M Leiker]

I agree as well! - I use them to mark everything, even things that don't 
get treated with chemicals.


One note: they work best if you mark hard non-porous surfaces (like the 
plastic cassettes) the day before to give the ink time to bind with the 
material.


Regards,
Merced

--On Wednesday, March 31, 2010 11:31 AM -0400 "Blazek, Linda" 
 wrote:



I have to agree.  They have never failed and work forever.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary,
Joseph Sent: Wednesday, March 31, 2010 11:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] lab markers KP

The only consistent lab pen I have used that will hold up under decal,
processing with various alcohol, xylene, hemo de is the KP marker plus.
I am sure there are several vendors, I use Mercedes.

Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010
10:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 45

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Coverslipping with SubX (Vanessa Avalos)
   2. Haunted cryostat/Thanks! (mtitf...@aol.com)
   3. GMS on decal tissue (Morken, Tim)
   4. questions (Webb, Dorothy L)
   5. Re: her2 validation (Pat Laurie)
   6. FREE MONEY! (Jackie M O'Connor)
   7. RE: questions (Garrison, Becky)
   8. Thermo's Excelsior Tissue Processor Program's
  (Akemi Allison-Tacha)
   9. re: cyto prep tech (Kim Tournear)
  10. her-2 neu validation (Debbie Nannenga)
  11. RE: questions (Tony Henwood)
  12. RE: RE: Leica Paraplast (connie grubaugh)
  13. (no subject) (Green JumpyOne)
  14. Fwd: [Histonet] (no subject) (Malika Benatti)
  15. RE: Thermo's Excelsior Tissue Processor Program's
  (Heckford, Karen - SMMC-SF)
  16. RE: RE: Leica Paraplast (Nails, Felton)
  17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett)
  18. Texas Society for Histotechnology April 23-25, 2010
  (kdwyer3...@aol.com)
  19. cassette labels erased by processor (Catherine Breen)
  20. RE:  her2 validation (Weems, Joyce)
  21. RE: cassette labels erased by processor (Sherwood, Margaret )
  22. FW: [Histonet] cassette labels erased by processor (Cheri Miller)
  23. Re: cassette labels erased by processor (Malika Benatti)


--

Message: 1
Date: Tue, 30 Mar 2010 11:15:38 -0700
From: "Vanessa Avalos" 
Subject: [Histonet] Coverslipping with SubX
To: "'HISTONET LISTS'" 
Message-ID: <01cad035$00d2f5b0$0278e1...@com>
Content-Type: text/plain;   charset="us-ascii"

Is anyone using SubX ? I am trying it out and am having some difficulty
coverslipping. I am using the Subx glue as directed since Acrymount didn't
seem to work as well. I still get a hazy film under the glass and am
getting big air bubbles as well. I can eventually get them out but its
just takes a while and you know how time is precious when you have a line
of slides to stain. The process is not as smooth as before. I really
would like this to work out for me and eliminate xylene.

Any suggestions??



Vanessa

Fax: 602-277-2134





--

Message: 2
Date: Tue, 30 Mar 2010 14:44:26 -0400
From: mtitf...@aol.com
Subject: [Histonet] Haunted cryostat/Thanks!
To: histo...@pathology.swmed.edu
Message-ID: <8cc9e5028adfb36-1500-2...@webmail-m010.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"



Thank you everyone who responded to my problem with a Leica CM 1850
cryostat turning itself off. About 11 people responded with tips. Thank
you!! The Histonet is great!! In answer to some enquirys:
1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts
for an hour. Jackie O' Conner asks if it is set to go back "on" after the
defrost. I have no idea. It defrosts well the rest of the time, and turns
itself back on. Brian Cornett-Early recommends changing the start device
on the compressor. 2) Someone else asked if power is getting to the
cryostat -  Yes, when it turns itself off, the on/off switch is on "off".
All you have to do is flip the switch and it starts pumping and  cooling.
3) Mari Ann Mailhiot with Leica recommends changing a cicui

[Histonet] retention of prosthetic valves

[Lise Matzke]

Hi there,
 
I was wondering what your hospital/centre's pathology policies indicate in 
terms of the time you retain prosthetic valve specimens?  Months?  Years?
 
 
thanks for your feedback,
 
Anne Marie

***CONFIDENTIALITY NOTICE***
This electronic message and any attachments are intended only for the use of 
the addressee and may contain information that is privileged and confidential.  
Any dissemination, distribution or copying of this communication by 
unauthorized individuals is strictly prohibited. If you have received this 
communication in error, please notify the sender immediately by reply e-mail 
and delete the original and all copies from your system.

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[Histonet] <29a3cb81288e6f4ba2c9b3c8015a9a130176a...@md1ev002.medimmune.com>

[Andrew Burgeson]

I agree that these pens are excellent.

Many years ago at the National Naval Medical center in
Bethesda, MD we had an entire run of cassettes marked with
"Xylene-proof" markers come out of the processor with all
the ink dissolved and NOTHING on the cassettes!

Fortunately, we had everything in order with a grossing log
and were religious about keeping the order of grossed
cassettes.

This was a scary deal.

SO I WILL NEVER TRUST THESE FOR CASSETTES, but I DO THINK
that KP markers are great and the MOHS tech at the last
practice and lab i worked for uses them.

KP definitely good. I don't recommend any you havent used
before...or test them first. A bad batch of ink and your
entire run is blank.



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[Histonet] RE: [BULK] Histonet Digest, Vol 76, Issue 45

[Tench, Bill]

For placentas, you will find that you get consistently good sections if you 
gross the placenta fresh, take the samples you will want from the cord, disc 
and make a membrane role (which is easily done if they are not fixed).  Fix 
your samples overnight and trim for blocks the next day. For the membrane role, 
grab the free edge of the membrane with a large forceps, role the membrane up 
on the forceps toward the disc, stick two pins through the space between the 
forceps, and cut from disc, then slightly release grip on forceps and slide 
role into formalin cup.

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
bill.te...@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, March 31, 2010 7:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 76, Issue 45

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Coverslipping with SubX (Vanessa Avalos)
   2. Haunted cryostat/Thanks! (mtitf...@aol.com)
   3. GMS on decal tissue (Morken, Tim)
   4. questions (Webb, Dorothy L)
   5. Re: her2 validation (Pat Laurie)
   6. FREE MONEY! (Jackie M O'Connor)
   7. RE: questions (Garrison, Becky)
   8. Thermo's Excelsior Tissue Processor Program's
  (Akemi Allison-Tacha)
   9. re: cyto prep tech (Kim Tournear)
  10. her-2 neu validation (Debbie Nannenga)
  11. RE: questions (Tony Henwood)
  12. RE: RE: Leica Paraplast (connie grubaugh)
  13. (no subject) (Green JumpyOne)
  14. Fwd: [Histonet] (no subject) (Malika Benatti)
  15. RE: Thermo's Excelsior Tissue Processor Program's
  (Heckford, Karen - SMMC-SF)
  16. RE: RE: Leica Paraplast (Nails, Felton)
  17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett)
  18. Texas Society for Histotechnology April 23-25, 2010
  (kdwyer3...@aol.com)
  19. cassette labels erased by processor (Catherine Breen)
  20. RE:  her2 validation (Weems, Joyce)
  21. RE: cassette labels erased by processor (Sherwood, Margaret )
  22. FW: [Histonet] cassette labels erased by processor (Cheri Miller)
  23. Re: cassette labels erased by processor (Malika Benatti)


--

Message: 1
Date: Tue, 30 Mar 2010 11:15:38 -0700
From: "Vanessa Avalos" 
Subject: [Histonet] Coverslipping with SubX
To: "'HISTONET LISTS'" 
Message-ID: <01cad035$00d2f5b0$0278e1...@com>
Content-Type: text/plain;   charset="us-ascii"

Is anyone using SubX ? I am trying it out and am having some difficulty
coverslipping. I am using the Subx glue as directed since Acrymount didn't
seem to work as well. I still get a hazy film under the glass and am getting
big air bubbles as well. I can eventually get them out but its just takes a
while and you know how time is precious when you have a line of slides to
stain. The process is not as smooth as before. I really would like this to
work out for me and eliminate xylene.

Any suggestions??

 

Vanessa

Fax: 602-277-2134

 



--

Message: 2
Date: Tue, 30 Mar 2010 14:44:26 -0400
From: mtitf...@aol.com
Subject: [Histonet] Haunted cryostat/Thanks!
To: histo...@pathology.swmed.edu
Message-ID: <8cc9e5028adfb36-1500-2...@webmail-m010.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"



Thank you everyone who responded to my problem with a Leica CM 1850 cryostat 
turning itself off. About 11 people responded with tips. Thank you!! The 
Histonet is great!!
In answer to some enquirys:
1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an 
hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I 
have no idea. It defrosts well the rest of the time, and turns itself back on. 
Brian Cornett-Early recommends changing the start device on the compressor.
2) Someone else asked if power is getting to the cryostat -  Yes, when it turns 
itself off, the on/off switch is on "off". All you have to do is flip the 
switch and it starts pumping and  cooling.
3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the 
side of the compressor. That is probably where we will start. Mari Ann works 
for Leica so it sounds like good advice.

Thank you everyone. Since it only breaks down about once a month, it will take

[Histonet] lab markers KP

[Madary, Joseph]

The only consistent lab pen I have used that will hold up under decal, 
processing with various alcohol, xylene, hemo de is the KP marker plus.  I am 
sure there are several vendors, I use Mercedes.

Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr, 
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, March 31, 2010 10:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 45

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Coverslipping with SubX (Vanessa Avalos)
   2. Haunted cryostat/Thanks! (mtitf...@aol.com)
   3. GMS on decal tissue (Morken, Tim)
   4. questions (Webb, Dorothy L)
   5. Re: her2 validation (Pat Laurie)
   6. FREE MONEY! (Jackie M O'Connor)
   7. RE: questions (Garrison, Becky)
   8. Thermo's Excelsior Tissue Processor Program's
  (Akemi Allison-Tacha)
   9. re: cyto prep tech (Kim Tournear)
  10. her-2 neu validation (Debbie Nannenga)
  11. RE: questions (Tony Henwood)
  12. RE: RE: Leica Paraplast (connie grubaugh)
  13. (no subject) (Green JumpyOne)
  14. Fwd: [Histonet] (no subject) (Malika Benatti)
  15. RE: Thermo's Excelsior Tissue Processor Program's
  (Heckford, Karen - SMMC-SF)
  16. RE: RE: Leica Paraplast (Nails, Felton)
  17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett)
  18. Texas Society for Histotechnology April 23-25, 2010
  (kdwyer3...@aol.com)
  19. cassette labels erased by processor (Catherine Breen)
  20. RE:  her2 validation (Weems, Joyce)
  21. RE: cassette labels erased by processor (Sherwood, Margaret )
  22. FW: [Histonet] cassette labels erased by processor (Cheri Miller)
  23. Re: cassette labels erased by processor (Malika Benatti)


--

Message: 1
Date: Tue, 30 Mar 2010 11:15:38 -0700
From: "Vanessa Avalos" 
Subject: [Histonet] Coverslipping with SubX
To: "'HISTONET LISTS'" 
Message-ID: <01cad035$00d2f5b0$0278e1...@com>
Content-Type: text/plain;   charset="us-ascii"

Is anyone using SubX ? I am trying it out and am having some difficulty
coverslipping. I am using the Subx glue as directed since Acrymount didn't
seem to work as well. I still get a hazy film under the glass and am getting
big air bubbles as well. I can eventually get them out but its just takes a
while and you know how time is precious when you have a line of slides to
stain. The process is not as smooth as before. I really would like this to
work out for me and eliminate xylene.

Any suggestions??

 

Vanessa

Fax: 602-277-2134

 



--

Message: 2
Date: Tue, 30 Mar 2010 14:44:26 -0400
From: mtitf...@aol.com
Subject: [Histonet] Haunted cryostat/Thanks!
To: histo...@pathology.swmed.edu
Message-ID: <8cc9e5028adfb36-1500-2...@webmail-m010.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"



Thank you everyone who responded to my problem with a Leica CM 1850 cryostat 
turning itself off. About 11 people responded with tips. Thank you!! The 
Histonet is great!!
In answer to some enquirys:
1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an 
hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I 
have no idea. It defrosts well the rest of the time, and turns itself back on. 
Brian Cornett-Early recommends changing the start device on the compressor.
2) Someone else asked if power is getting to the cryostat -  Yes, when it turns 
itself off, the on/off switch is on "off". All you have to do is flip the 
switch and it starts pumping and  cooling.
3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the 
side of the compressor. That is probably where we will start. Mari Ann works 
for Leica so it sounds like good advice.

Thank you everyone. Since it only breaks down about once a month, it will take 
a long time to determine if the problem is fixed.

Michael Titford
Pathology USA
Mobile AL USA



--

Message: 3
Date: Tue, 30 Mar 2010 11:52:17 -0700
From: "Morken, Tim" 
Subject: [Histonet] GMS on decal tissue
To: histonet 
Message-ID:

<1aaf670737f193429070841c6b2add4c013b16c...@exmbmcb15.ucsfmedicalcenter.org>

Content-Type: text/plain; charset=us-ascii

Has anyone seen or heard of problems with Gr

[Histonet] RE: lab markers KP

[Blazek, Linda]

I have to agree.  They have never failed and work forever.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph
Sent: Wednesday, March 31, 2010 11:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] lab markers KP

The only consistent lab pen I have used that will hold up under decal, 
processing with various alcohol, xylene, hemo de is the KP marker plus.  I am 
sure there are several vendors, I use Mercedes.

Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, March 31, 2010 10:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 45

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Today's Topics:

   1. Coverslipping with SubX (Vanessa Avalos)
   2. Haunted cryostat/Thanks! (mtitf...@aol.com)
   3. GMS on decal tissue (Morken, Tim)
   4. questions (Webb, Dorothy L)
   5. Re: her2 validation (Pat Laurie)
   6. FREE MONEY! (Jackie M O'Connor)
   7. RE: questions (Garrison, Becky)
   8. Thermo's Excelsior Tissue Processor Program's
  (Akemi Allison-Tacha)
   9. re: cyto prep tech (Kim Tournear)
  10. her-2 neu validation (Debbie Nannenga)
  11. RE: questions (Tony Henwood)
  12. RE: RE: Leica Paraplast (connie grubaugh)
  13. (no subject) (Green JumpyOne)
  14. Fwd: [Histonet] (no subject) (Malika Benatti)
  15. RE: Thermo's Excelsior Tissue Processor Program's
  (Heckford, Karen - SMMC-SF)
  16. RE: RE: Leica Paraplast (Nails, Felton)
  17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett)
  18. Texas Society for Histotechnology April 23-25, 2010
  (kdwyer3...@aol.com)
  19. cassette labels erased by processor (Catherine Breen)
  20. RE:  her2 validation (Weems, Joyce)
  21. RE: cassette labels erased by processor (Sherwood, Margaret )
  22. FW: [Histonet] cassette labels erased by processor (Cheri Miller)
  23. Re: cassette labels erased by processor (Malika Benatti)


--

Message: 1
Date: Tue, 30 Mar 2010 11:15:38 -0700
From: "Vanessa Avalos" 
Subject: [Histonet] Coverslipping with SubX
To: "'HISTONET LISTS'" 
Message-ID: <01cad035$00d2f5b0$0278e1...@com>
Content-Type: text/plain;   charset="us-ascii"

Is anyone using SubX ? I am trying it out and am having some difficulty
coverslipping. I am using the Subx glue as directed since Acrymount didn't
seem to work as well. I still get a hazy film under the glass and am getting
big air bubbles as well. I can eventually get them out but its just takes a
while and you know how time is precious when you have a line of slides to
stain. The process is not as smooth as before. I really would like this to
work out for me and eliminate xylene.

Any suggestions??



Vanessa

Fax: 602-277-2134





--

Message: 2
Date: Tue, 30 Mar 2010 14:44:26 -0400
From: mtitf...@aol.com
Subject: [Histonet] Haunted cryostat/Thanks!
To: histo...@pathology.swmed.edu
Message-ID: <8cc9e5028adfb36-1500-2...@webmail-m010.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"



Thank you everyone who responded to my problem with a Leica CM 1850 cryostat 
turning itself off. About 11 people responded with tips. Thank you!! The 
Histonet is great!!
In answer to some enquirys:
1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an 
hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I 
have no idea. It defrosts well the rest of the time, and turns itself back on. 
Brian Cornett-Early recommends changing the start device on the compressor.
2) Someone else asked if power is getting to the cryostat -  Yes, when it turns 
itself off, the on/off switch is on "off". All you have to do is flip the 
switch and it starts pumping and  cooling.
3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the 
side of the compressor. That is probably where we will start. Mari Ann works 
for Leica so it sounds like good advice.

Thank you everyone. Since it only breaks down about once a month, it will take 
a long time to determine if the problem is fixed.

Michael Titford
Pathology USA
Mobile AL USA

[Histonet] Wanted IEC-CTD cryostat

[Eric Baltazar]

Hello fellow Histonetters! I'm looking for cryostat machine model IEC-CTD.
They're the old ice cream box type used for frozen section, in case you have
this park on your storage areas and not using it, I'm very much willing to
purchase it regardless of its condition.

You can call me at 323-4789871 or return me an email @
drmtec...@gmail.comfor this inquiry update.Thank you and looking
forward for a favorable reply
soon!
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[Histonet] LC3-II Autophagy antibodies for IHC ?

[Perry, Samuel]

Hi,
Tried post this the other day but I didn't see it go up.  Can you make sure it
gets on the mailing list.  Thanks! -Sam

"Hi All,
I am trying to do IHC staining on FFPE mouse and human tissue to look for
autophagy.
Can anyone a recommend a good antibody which recognizes human or mouse LC3? In
particular, I would hope that it recognizes only the autophagy specific
membrane-bound  LC3-II form not total LC3. Does such an antibody exist?
Thanks -Sam Perry 
Dana-Farber Cancer Institute
Research Technician "



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FW: [Histonet] cassette labels erased by processor

[Cheri Miller]

YES! That is why I use pencil only. I had a processor full of blank/ unlabeled 
cassettes because the lot# of the pens was bad. There is no way of knowing if 
the ink is reagent proof until a disaster like you have occurs.  You don't 
always know if the ink lot passed its QC with absolute certainty. Pencil is 
fail proof. I hope your day gets better, Cheri

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine Breen
Sent: Wednesday, March 31, 2010 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cassette labels erased by processor

I

PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you 
are not the addressee intended / indicated or agent responsible for delivering 
it to the addressee, you are hereby notified that you are in possession of 
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Re: [Histonet] cassette labels erased by processor

[Malika Benatti]

Hi there,

I would suggest to use a pencil rather than a marker pen to label  
cassettes when processing cassette with the Sakura VIP , my lab had a  
number of the so called solvent proof pen on trial and have yet to  
find one that survive processing.





Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London

 " ... Smile it confuses people ..."

On 31 Mar 2010, at 15:13, "Catherine Breen"  wrote:

I am looking for help solving a lab mystery.  The cassettes in our  
lab are labeled with an SP Securline Marker II and then processed in  
a Sakura VIP processor.  Two weeks ago the entire batch came out  
labeled much more lightly than usual, some to the point of where the  
label was completely effaced.
Our current theory is that an acid cleanser (Citronox) or possibly  
another acid was accidentally introduced into the processor.

Has any lab experienced this problem before, especially with Citronox?

Thank you.


Best Weight Loss Program - Click Here!
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RE: [Histonet] cassette labels erased by processor

[Sherwood, Margaret]

I had similar issues with all of the marking pens out there.  We finally
switched to Tissue-Tek Marking pencils #4160 and have not had a problem since.
Plus they never "dry" out!

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine Breen
Sent: Wednesday, March 31, 2010 10:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cassette labels erased by processor

I am looking for help solving a lab mystery.  The cassettes in our lab are
labeled with an SP Securline Marker II and then processed in a Sakura VIP
processor.  Two weeks ago the entire batch came out labeled much more lightly
than usual, some to the point of where the label was completely effaced.  
Our current theory is that an acid cleanser (Citronox) or possibly another acid
was accidentally introduced into the processor.
Has any lab experienced this problem before, especially with Citronox? 

Thank you.


Best Weight Loss Program - Click Here!
Weight Loss Program
http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOA
AYAAADNAAAEUlAqCWY=
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[Histonet] RE: her2 validation

[Weems, Joyce]

>From June-15, 2009 CAP checklist



ANP.22997   Phase I N/A  YES  NO

If the laboratory performs HER2 testing (HER2  protein over-expression by 
immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization 
[e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented appropriate 
validation for the assay(s)?  

NOTE:  Initial test validation must be performed on a minimum of 25 cases 
(recommended 25-100). Validation may be performed by comparing the results of 
testing with a validated alternative method (i.e. IHC vs. FISH) either in the 
same laboratory or another laboratory, or with the same validated method 
performed in another laboratory; validation testing must be done using the same 
set of cases in both labs.

If specimens are fixed in a medium other than 10% neutral buffered formalin, 
the validation study must show that results are concordant with results from 
formalin-fixed tissues. 

If  significant changes are made in testing methods (e.g., antibody clone, 
antigen retrieval protocol or detection system, FISH probe or pretreatment 
protocol), revalidation is required.

This checklist item applies to laboratories that perform the technical testing 
of specimens for HER2 amplification. Patient specimens should be fixed in the 
same manner as the specimens used for the validation study(ies).

*CISH =  chromogenic in-situ hybridization; SISH = silver-enhanced in-situ 
hybridization


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

Confidentiality Notice:
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property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.


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[Histonet] cassette labels erased by processor

[Catherine Breen]

I am looking for help solving a lab mystery.  The cassettes in our lab are 
labeled with an SP Securline Marker II and then processed in a Sakura VIP 
processor.  Two weeks ago the entire batch came out labeled much more lightly 
than usual, some to the point of where the label was completely effaced.  
Our current theory is that an acid cleanser (Citronox) or possibly another acid 
was accidentally introduced into the processor.
Has any lab experienced this problem before, especially with Citronox? 

Thank you.


Best Weight Loss Program - Click Here!
Weight Loss Program
http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAADNAAAEUlAqCWY=
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[Histonet] Texas Society for Histotechnology April 23-25, 2010

[kdwyer3322]

Histonetters, 
It is not too late to join us for the 2010 TSH meeting in Houston Texas April 
23-25, 2010. 

The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors 
and Attendees.  

Workshops begin Friday April 23, 2010 at 8:00am.  

There is still plenty of time to register and get a hotel room to enjoy 2 full 
days of workshops and symposiums.  

If you would like a program go to txsh.org  or contact me via this e-mail.  

Thanks, 
TSH Convention Committee



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[Histonet] Thermo Fisher Excelsior Tissue Processor Programs

[Ann Bennett]

At one point we looked at getting an Excelsior and I spoke with our sales rep 
and he said all the reagents we use in our processor now are absolutely fine.  
He mentioned nothing about not being able to use Reagent alcohols or xylene 
substitutes.  Hope this helps - have a happy histo day!
 
 



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RE: [Histonet] RE: Leica Paraplast

[Nails, Felton]

There is about three brands of paraplast and they all cut differently. In one 
of my labs I use paraplast plus and it ribbons very well however the blocks 
have to be colder then if you are using TissuePrep from Fisher.
Leica may have sent you one of the other types of paraplast. (paraplast, 
Paraplast Plus, Paraplast Xtra) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of connie grubaugh
Sent: Tuesday, March 30, 2010 9:58 PM
To: ddree...@sbcglobal.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Leica Paraplast


Hi all, I questioned the Leica paraplast that we have received too.  It is real 
gummy and sticky.  Takes me forever to cut. I asked and was informed that it is 
the same stuff we have always got and there is no difference. Except all of us 
techs have noticed a big difference. 



Connie G.



 

> Date: Tue, 23 Mar 2010 11:29:03 -0700
> From: ddree...@sbcglobal.net
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Leica Paraplast
> 
> 
> Hi Kristen,
> We were using the Paraplast Xtra and switched to something else after we 
> noticed a difference in the quality of the paraffin. The tissues weren't 
> cutting as well and the paraffin seemed to be "gritty". We found we were 
> going through many more blades due to nicks and scratches and many times had 
> to switch blades mid-block.
> 
> 
> From: histonet-requ...@lists.utsouthwestern.edu 
>  Message: 4
> Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT)
> From: kristen arvidson 
> Subject: [Histonet] Leica Paraplast
> To: histonet 
> Message-ID: <94596.93077...@web65707.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> Has anyone who uses paraplast (we use the basic one) noticed a change in the 
> quality of your tissue?  I have recently found out that they have changed 
> manufacturing sites in the past couple of months.  I am having on and off 
> issues with my skin specimens that have been going on for about 2 months or 
> so.  Thought there may be a correlation.  Any thoughts? 
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RE: [Histonet] Thermo's Excelsior Tissue Processor Program's

[Heckford, Karen - SMMC-SF]

Pretty much any problems I have had with the Excelsior and I have been using 
one for 4 years now is with Pathologists loading it incorrectly.  I use reagent 
alcohol in it all the time, with no problems.   I prefer using xylene in my 
processor.  Every single time I have switched and started using a non-xylene 
sub.  I have had nothing but problems.  If I use a non-xylene I save it for the 
stainer. 
Take it easy,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi 
Allison-Tacha
Sent: Tuesday, March 30, 2010 4:04 PM
To: histo net
Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's

Hi All of you in Histoland,
 
I am working with a facility that recently purchased a Thermo Excelsior Tissue 
Processor.  They have had processing problems with several of their specimens 
using non-xylene clearing agent.  These issues were with both bx's and larger 
tissues.  The program and the non-xylene clearing agent was recommended by the 
technical staff at Thermo. 
 
The histology staff have switched back to using xylene verses the non-xylene 
clearing agent, and most of the issues have disappeared.
 
Also, the histotech's were using reagent alcohol, histological grade.  This 
grade of alcohol is denatured with methyl alcohol.  The sales representative 
was in and informed us that this type of alcohol has damaging effects on 
the instrument.  The representative will be coming in to flush out the system 
and reprogram the instrument next Monday.  
 
I looked over the current VIP program which is being used, and the program, 
timing and temperatures are a little different from most hospital and private 
laboratories I have worked with.  We are going to use basically the same 
program for the Excelsior, that we use on the VIP.
 
I would like to know what other Excelsior Tissue Processor users that use 
xylene have for their programs.  It would be great to compare the reagents, 
programs, timing, and temperatures for Routine overnight runs and for Rapid 
Biopsy Runs.  Thank you in advance for your assistance.

Akemi Allison-Tacha BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com


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Fwd: [Histonet] (no subject)

[Malika Benatti]

Dear Histonet list manager

Is they anyway you could filter/block spam such as this one from be  
sent out to the Histonet list.


Cheers

Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London

 " ... Smile it confuses people ..."

Begin forwarded message:


From: Green JumpyOne 
Date: 31 March 2010 09:52:42 GMT+01:00
To: , , >

Subject: [Histonet] (no subject)




http://www.trainedlabor.com/H6UH4Yh1UJ.html

_
The New Busy is not the old busy. Search, chat and e-mail from your  
inbox.

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[Histonet] (no subject)

[Green JumpyOne]

http://www.trainedlabor.com/H6UH4Yh1UJ.html
  
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