[Histonet] CPT coding

[histotech]

Hi Histonetters!

We have run into a question about CPT charges and I was hoping to get a more
definitive answer than what we currently have.  I know if you have two
tonsils in one container, one with a stitch and one without, you can charge
for both tonsils.  How about if you have one container with a placenta and a
stillborn fetus?  One suggestion is to charge an 88307 and the other is to
charge 88300 AND an 88307 (to cover the gross only on the fetus and the
tissue that was submitted for the placenta).  What is the standard, shall I
say legal?, way of charging for these specimens?  Must you choose one of the
charges or are we allowed to use both charges?

Thanks for any light you can shed on this!

Michelle


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[Histonet] New job opportunities

[Jan . Minshew]

Hello everyone,

Leica Microsystems is adding new positions and would like to hear from
those of you who might be interested.  The positions are for Field Support
Specialists (FSS) and will be based in Arizona, Washington state, New
England, Florida and Montreal.  Applicants for the Montreal position must
be fluent in both French and English.  Some of the responsibilities include
in-field support for IHC, conducting demonstrations and post-sales customer
training, performing validations and optimizations and providing technical
support for remote problem solving.

If you are interested in trying something new and different and feel that
you are qualified, please contact Linda Jordan, HR Recruiter at
hrco...@leica-microsystems.com.

Kind regards,

Jan Minshew
Marketing Manager
Leica Microsystems
Biosystems Division
2345 Waukegan Road
Bannockburn, IL 60015

Office:  847.405.7051
Cell: 847.970.8468
Fax: 847.405.6560

www.leica-microsystems.com


Click Here for this month's special offers!
http://www.leica-microsystems.com/bsdspecial


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[Histonet] Masson Trichome Staining: Can I use Fast Green?

[cscampbe]

Hi Histonet,

I am currently using the protocol from Stainsfile for staining heart
tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm

My lab had Fast Green readily available so I substituted it for Light
Green in Solution C. The slides have not been coming out well. I failed to
account for the difference in timing between FG and LG - running the slide
in FG for the full 10 minutes. Has anyone used FG in Masson's Trichrome?
If so, how long did it take to get the desired colors in the solution? Or,
is Fast Green a poor substitute for Light Green?

Thanks for your advice!
-Colin


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[Histonet] Histo Aid / Histotech

[Scherer, Kris]

We would like to hire a histology aid.  What are the educational requirements 
for a histology aid, compared to a histotechnician?


--Kris
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[Histonet] Illinois Annual Meeting

[Rae Staskiewicz]

This year the Illinois Society for Histotechnologists is holding their
Annual Spring meeting at Eaglewood Resort in Itaska, Illinois. The dates are
May 13-14, 2010. Unfortunately it seems as though we are trying to follow
the example of the Republican National Committee and have an incorrect phone
number for room reservations at the resort. The correct number is
1-877-285-6150. Be sure to mention that you are with the ISH when making
your reservation in order to receive the convention rates. Reservations must
be made by April 12 in order to receive the reduced rates. Check out the
Eaglewood Resort at www.eaglewoodresort.com
  to see all of the amenities waiting for
you! If you would like an electronic brochure, contact myself at
raest...@grics.net. 

 

 

Rae Ann Staskiewicz

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[Histonet] eProtocols

[Shea's]

Our Pathologist found it more time consuming to delete lines that were 
non-applicable than to dictate what they do want from the CAP Cancer Protocols:

1. I copied and pasted the commonly used protocols into the canned text 
dictionary (in meditech, magic) then each pathologist will edit it to meet 
their usual needs, and save it again (giving it a name they will remember). To 
use it, they go into the Diagnosis data section, type the canned text, then 
they still add/delete lines that are applicable, but it is still a bit more 
user friendly. They each have a thick binder notebook of the protocols in print 
to follow along if needed.

2. Now that we use dragon voice recognition, they have all made up "macros" for 
the most commonly used ones. They will go to the diagnosis data section and 
voice the command for the protocol. A macro/canned text version will appear. 
They will also have a printed copy from CAP Protocol to follow along if they 
need to add or delete anything.

Either way they find it easier than deleting lines. Also I should add, this is 
easiest for them since we do not have a pathology secretary or transcriptionist.

Jan
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[Histonet] Tungesten Carbide Knives and araldite

[Eridana]

I have a tiny project using araldite on mouse nerves and wondered if anyone 
could recommend the tungsten carbide knife that Ted Pella cat #121-50 or any 
other company sells. I cut methacrylate ages ago with glass knives but wonder 
if the tungesten carbide blade might be a  easier and way less expensive option 
than a $9k Leica glass knife breaker.  I used the Sorvall breaker  but it looks 
to be discontinued.

If anyone has an opinion on araldite, I would appreciate that too.

Thanks

Donna

Donna Harclerode HT,HTL,QIHC (ASCP),SLS 
Histology Core Manager 
UCSD, Dept of Pathology 
9500 Gillman Drive 
BSB 2010 
San Diego, CA 92093
858 534 7438


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[Histonet] fixtives

[mohamed abd el razik]

are you using bouin's , susa or formol zinker?? how many times do you use the 
same fixtive? i know that zinker need to be freshly prepaired
 
thanxs 
 
 
mohamed



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[Histonet] Re: Histonet Digest, Vol 77, Issue 10

[Hocking, Mark]

D
Mark Hocking
Symphony Technical Applications Specialist
Ventana Medical Systems, Inc.
A member of the Roche group

Message sent via blackberry

- Original Message -
From: histonet-boun...@lists.utsouthwestern.edu 

To: histonet@lists.utsouthwestern.edu 
Sent: Thu Apr 08 13:03:49 2010
Subject: Histonet Digest, Vol 77, Issue 10

Send Histonet mailing list submissions to
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To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: frozen lung sections (Tony Henwood)
   2. Re: BREAST IMPLANTS AND HARDWARE (Anne van Binsbergen)
   3. Traveling Histo Tech (Marshall, Kimberly)
   4. RE: TX Society meeting? (Nita Searcy)
   5. trich/bouin's/FS/Inst cleaning/mast cells (John PJ Coleman)
   6. eProtocols (Weems, Joyce)
   7. Sestrin 2 (Bernard Martin)
   8. Re: eProtocols (dkb...@chs.net)
   9. RE: Traveling Histo Tech (Cheri Miller)
  10. RE: Travelling Histo Tech (Edwards, Richard E.)
  11.  (Thotakura, Anil Kumar)
  12. CD 25 protocol on Benchmark (Gudrun Lang)
  13. CD138 IHC (julia hough)
  14. Re: eProtocols (Bill)
  15. Re: Just three months? (Michael LaFriniere)


--

Message: 1
Date: Thu, 8 Apr 2010 11:16:31 +1000
From: "Tony Henwood" 
Subject: RE: [Histonet] frozen lung sections
To: "Molinari, Betsy" ,

Message-ID: 
Content-Type: text/plain; charset="us-ascii"

Betsy,

I would dissolve the OCT in Hanks solution (or similar cell culture
medium - not saline). Place in dilute OCT (50/50 with distilled water),
place on stirrer until the lung settles in the OCT 30-60min. Blot with
absorbent paper, refreeze in fresh OCT. It should cut quite well.

I have used this previously on some tissue that was received the same
way. It seems that the OCT had "freeze-dried" prior to receipt in my
lab.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Molinari, Betsy
Sent: Thursday, 8 April 2010 12:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] frozen lung sections


Hi all,

 I have received some lung samples on OCT. These lungs have not been in
PBS or any fixative. They went directly from dissection into OCT and
frozen.

 I cannot get a section. The temp of the cryostat is -20. I have done
some reading and it seems the best results come from immersion in PBS,
then 4% paraformaldehyde then into sucrose. Can anyone clarify the
procedure or offer advice? I am unfamiliar with this technique since
most of my frozen sectioning is heart tissue and it does not present the
same problem as the lung.

Thanks,

Betsy



Betsy Molinari HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812



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Message: 2
Date: Thu, 8 Apr 2010 08:11:50 +0400
From: Anne van Binsbergen 
Subject: Re: [Histonet] BREAST IMPLANTS AND HARDWARE
To: "Vickroy, Jim" 
Cc: "Histonet@lists.utsouthwestern.edu"

Message-ID:

Content-Type: text/plain; charset=ISO-8859-1

your path dept is being 'used' as a tracking system for these items - the
charge is perfectly justifiable - we do it all the time
tell the plastics guys to set up their own tracking system for these sample

Re: [Histonet] Just three months?????

[Michael LaFriniere]

FYI
The Grandfather clause still stands as it always had
 
 
 
Michael R. LaFriniere
Executive Director
Cytology Services of Maryland (CSM)
301-206-2555 ext 27  301-206-2595 fax
michael.lafrini...@csmlab.com


>>> On 4/1/2010 at 8:46:10 PM, "Joe Nocito"  wrote:
I see that now, wasn't the first time I was wrong today, probably wouldn't 
be the last either

- Original Message - 
From: "Jeffrey Silverman" 
To: 
Sent: Wednesday, March 31, 2010 5:03 PM
Subject: [Histonet] Just three months?


CAP is now saying no more gross processing of small things that are entirely 
submitted- it's all gross examination now whether we mean straining 
currettings into a cassette or dissecting a complex cancer resection. Dumb 
as all get out if you ask me.

As for the three month training thing, Joe, I'm not so sure about that. They 
seem to spell out specific amounts of college education required IN ADDITION 
to training in the laboratory.
The requirement first spells out two education choices-
1: an earned associates degree in medical laboratory science

OR 2:Education/training equivalent to the above that includes at least 60
semester
hours or equivalent from an accredited institution. This education
MUST (caps mine) include 24 semester hours of medical laboratory technology 
courses, OR 24
semester hours of science courses that includes 6 semester hours of
chemistry,
6 semester hours of biology, and 12 semester hours of chemistry,
biology
or medical laboratory technology in any combination.

So in addition to all the college courses, which we all know you need to 
count and measure six endoscopic biopsies and put them in a cassette, much 
more training and experience needed than what an unregistered on the job 
tech needs to orient and embed them properly LOL!!! CLIA then requires that 
the
individual must have (additional) laboratory training including either 
completion of
a clinical laboratory training program approved or accredited by
the ABHES, NAACLA, or other organization approved by HHS (note that
this training may be included in the 60 semester hours listed
above),
OR at least 3 months documented laboratory training in each
specialty in which the individual performs high complexity testing.

Now there are grandfathering clauses in CLIA which may enable folks (like 
myself) to continue to gross. I haven't digested that yet, but I'm ready to 
get that job in Pathmark if necessary.

Sheesh, does it ever end?

Jeff Silverman
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Re: [Histonet] eProtocols

[Bill]

At 9:54 AM -0400 4/8/10, Weems, Joyce wrote:
>Are any of your pathologists using the Cancer eProtocols provided by CAP? If 
>so you would you let me know of your experiences and how you interfaced it 
>with your LIS?<

We export data from our LIS and pick it up in Word merge templates. We then use 
autotext to add the appropriate protocol info (modified) for the diagnosis. 

Bill


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[Histonet] CD138 IHC

[julia hough]

Dear all.
 
Does anyone have a protocol for the immunohistochemistry staining of CD138 on 
paraffin embedded mouse tibia, including the fixation and decalcification 
protocol?
 
Thanks
 
BW
 
Julia 
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[Histonet] CD 25 protocol on Benchmark

[Gudrun Lang]

Hi Benchmark-users!

Can someone of you provide me with a protocol for CD 25 for FFPE tissue. We
have purchased the antibody from Cell marque and have found nuclear staining
in trephine bone marrow biopsy with mastocytosis. This specimen was
decalcified with formic acid.

Used protocol: CC1 mild, 1:10 for 40 min

I would be happy about any help.

 

Gudrun Lang

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[Histonet]

[Thotakura, Anil Kumar]

Hi All,

I have a problem with immunohistochmesitry. I am have raised my own antibody
and it is not commercially available. This monoclonal antibody works fine
for western blot. When it come to immunohistochemistry on mice liver frozen
section, it is binding every where. I can see staining in my control as
well.

Any suggestion or guess's from you guys?

Anil 


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[Histonet] RE: Travelling Histo Tech

[Edwards, Richard E.]

So you  could sum it  up thus:-  "Have knife, will slice for a price".

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: 08 April 2010 16:17
To: Marshall, Kimberly; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Traveling Histo Tech

Only to say I am envious, good luck and have fun!

Cheryl Miller HT ASCP cm
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148



PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you 
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[Histonet] RE: Traveling Histo Tech

[Cheri Miller]

Only to say I am envious, good luck and have fun!

Cheryl Miller HT ASCP cm
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148



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Re: [Histonet] eProtocols

[DKBoyd]

I have printed off the  CAP worksheets off the web site.  We use them as 
work sheets for the pathologist.  He checks off and fills in what is 
pertinent to the case, then the transcriptionist transcribes the Synoptic 
Report into the final report directly under the Final Diagnosis.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







"Weems, Joyce"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
04/08/2010 09:54 AM

To
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] eProtocols






Hello Everyone,

Are any of your pathologists using the Cancer eProtocols provided by CAP? 
If so you would you let me know of your experiences and how you interfaced 
it with your LIS?

Thanks much!, j


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


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[Histonet] Sestrin 2

[Bernard Martin]

Hi all,

I asked this a couple weeks ago, but thought I'd give it one more try- 

has anyone had any experience with Sestrin 2 for IHC?  

I can't find anything in the literature, and thought maybe some has tried it.  
There are ones available, but they all use the same picture, and I am concerned 
perhaps it doesn't work well.  Funds are limited so we would like to get one 
that someone has had some success with.  Thanks for any info you could provide!

Bern


  

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[Histonet] eProtocols

[Weems, Joyce]

Hello Everyone,

Are any of your pathologists using the Cancer eProtocols provided by CAP? If so 
you would you let me know of your experiences and how you interfaced it with 
your LIS?

Thanks much!, j


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


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for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.
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[Histonet] trich/bouin's/FS/Inst cleaning/mast cells

[John PJ Coleman]

Trichrome: Our Masson Trichrome procedure uses a combined  
phosphotungstic/phosphomolybdic acid step for 20 minutes @ RT without  
a rinse after, our smooth muscle stays RED..


Bouin's: Working in a rat research lab for years, we fixed all tissue  
in bouins for 24 hours, rinsed in water for about an hour and stored  
in 70% alcohol till processing.  Is all your tissue post fixed in  
bouins or just selected items? Why not Bouin's as a primary fixative?  
We got great results on PTAH, Trichrome, Luxol Fast Blue stains.


Frozens: CAP says monitor FS times, if 90% are not within 20 minutes,  
document the evaluation of why.   ANP.11820


Instrument cleaning: We use TBQ which is virucidal and does not rust  
instruments like bleach does.


Mast cells: We have not had unintentional mast cell staining on our  
fleet of Ultras using the either Ultraview or the alk phos kits.



John PJ Coleman, HT(ASCP)QIHC






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RE: [Histonet] TX Society meeting?

[Nita Searcy]

Website has all of the information

Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438

>>> "Feher, Stephen"  4/7/2010 4:13 PM >>>
Contact kdwyer3...@aol.com.  April 23-25, Houston, TX.  Be sure to sign up for 
the "Designing a LEAN Pathology Lab from Scratch" workshop on Saturday, April 
24. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, April 06, 2010 5:09 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] TX Society meeting?


 
 Any word on Texas' regional histology meeting?  I'm looking for dates and the 
registration info for my team--
 
Thanks!
 
Cheryl Kerry, HT(ASCP)
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TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
END:VCARD

BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org
N:Searcy;Nita
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[Histonet] Traveling Histo Tech

[Marshall, Kimberly]

Hello all,

  A few years back I went on vacation and we used a traveling Histo tech to 
cover.  Back then I thought it was a neat idea and said to myself, when the 
kids are grown and out of the house I am gonna do that.  Well that time has 
come, so before I started making calls and researching it I wanted to ask my 
fellow Histo folks what they know about it.  Is there still a market for 
traveling techs?  Any information about this would sure be appreciated.
Thanks in advance
Kimberly Marshall HT(ASCP)

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