[Histonet] IgG4 source

[Joao Pessoa]

I need to find a source for an IgG4 antibody for use with IHC studies on
human FFPE tissue.  Any ideas?  My preference would be for a
concentrated IVD.

Thanks,

Joao
Histo Tech
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[Histonet] nonspecific staining in liver

[Jennifer Campbell]

Hi All,
 
 I am having some problems with marked nonspecific staining in liver
when running my CD3 and CD79a.  I am using an avidin and biotin block,
as well as a protein block.  Any other suggestions as to what I could do
to eliminate this?
 
Thanks in advance,
 
Jennifer Campbell
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[Histonet] Re: Histonet Digest, Vol 78, Issue 8

[Stephanie Rodriguez]

Tonia,

If you are interested in sending specimens out for validation, our
laboratory currently provides this service for HER2 testing by IHC and FISH.
Information is on our website at www.phenopath.com, under ŒDiagnostic
Services¹ at the top of the home page.

Good luck!

Stephanie Rodriguez, BS, HTL(ASCP), QIHC
Senior Molecular Technologist, FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA


On 5/7/10 5:47 AM, "histonet-requ...@lists.utsouthwestern.edu"
 wrote:

> Message: 7
> Date: Thu, 6 May 2010 11:38:42 -0700
> From: "Morken, Tim" 
> Subject: RE: [Histonet] Responses to IHC CAP Validation question
> To: "tonia.richm...@gracepathology.com"
>  , "McMahon, Loralee A"
>  
> Cc: "histonet@lists.utsouthwestern.edu"
>  , "thisis...@aol.com"
>  
> Message-ID:
>  <1aaf670737f193429070841c6b2add4c018766a...@exmbmcb15.ucsfmedicalcenter.org>
>  
> Content-Type: text/plain; charset=utf-8
> 
> Tonia, obviously you have to validate your systems so you need to do some
> validation ahead of time with non-patient samples. You can validate your
> instruments and reagents on tissue arrays (you can even get commercial
> validated arrays for ER, PR and Her2). I suggest Pantomics (www.pantomics.com)
> for excellent quality arrays of all kinds.
> 
> To validate the actual test, once you start up you will have to do some
> parallel testing by testing in-house and sending paired samples out to be
> tested. Then document the results of each. When you can show concordance of
> results over a set of samples (for most antibodies, 10 or more, 5 pos and 5
> negative) that test can be considered validated. For ER, PR and Her2 you will
> need to have concordance on many more samples (25 - 100). I suggest, if
> possible, taking extra tissue from patient samples and making either tissue
> arrays or sausage arrays to do this testing. It will drastically lower the
> number of slides you have to test in house and to send out to be tested.
> 
> It may be possible to partner with another lab to do this testing. As part of
> proficiency testing you can ask other labs for samples (wet tissue or
> unstained slides) to validate your results against theirs. Some labs are
> looking for partners to do this kind of testing. You then each validate each
> others tests.
> 
> Tim Morken
> Supervisor, Histology / IPOX
> UCSF Medical Center
> San Francisco, CA
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> tonia.richm...@gracepathology.com
> Sent: Thursday, May 06, 2010 8:28 AM
> To: McMahon, Loralee A
> Cc: histonet@lists.utsouthwestern.edu; thisis...@aol.com
> Subject: RE: [Histonet] Responses to IHC CAP Validation question
> 
> 
>If  you  are a brand new lab, how do you validate IHC if you are no=
>yet  receiving patient specimens?  Can the validation be done on cont   rol
> tissues only?
> 
> 
> 
> 
>Sincerely,
> 
> 
>Tonia Richmond, AS, HT (ASCP)
>Chief Operations Officer =aboratory
>Grace Pathology
>PH:  (501) 765-7367
>Email: =]tonia.=chm...@gracepathology.com



This e-mail message, including any attachments, is for the sole use of the 
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review, use, disclosure or distribution is prohibited. If you are not the 
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recipient, please contact the sender by e-mail and destroy all copies of the 
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[Histonet] DiD retrograde tracing study. Vibratome or cryostat

[Nicole Cosenza]

We are beginning a retrograde tracing study in rats. We injected DiD 
into the rats and in 4 weeks we will perfuse and harvest the brainstems. 
My question is, is vibratome sectioning better than cryostat sectioning? 
The literature that accompanied the dye said either is an option, but 
some have noted dye leaching out when cyrostat sectioned. How common is 
this problem?  If vibratome is the better choice, is there a good 
embedding protocol?



Nicole
Southern Illinois University School of Medicine

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[Histonet] Re: Special stain to demonstrate silver

[Johnson, Teri]

Maria,

I don't know of any particular procedure or stain to demonstrate silver, but I 
got to thinking wouldn't it reduce with formalin fixation to a visible state? 
Or maybe strong sunlight?

Kind regards,

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO


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RE: [Histonet] Special stain to demonstrate Silver

[Troutman, Kenneth A]

Hi Fanny,

I don't know of one, but perhaps you could modify a GMS.  I don't think you 
would need to oxidize it at the beginning with the periodic acid or chromic 
acid.  Make the working Methenamine silver solution without the silver.  (Don't 
forget to add the Borate) and try and visualize it that way.  I probably 
wouldn't do the hypo and gold chloride until I looked at it under the scope to 
see if it was actually staining, you wouldn't want to remove the silver you are 
trying to visualize.  I would be careful in interpreting that because I don't 
know what else it might be picking up--it might pick up other metals.  That's 
just a suggestion off the cuff.  If you try that, let us know what happens!  
Good luck!

Thanks,

Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN  37232
615-343-9134

Message: 15
Date: Fri, 7 May 2010 07:00:25 -0500
From: "DeGuzman, Maria" 
Subject: [Histonet] Special stain to demonstrate Silver
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<5476245379016b4d8212e8bcd11ecffbb77a5...@amwpvex01.kci.com>
Content-Type: text/plain; charset="us-ascii"
Hello Histoneters,
Is there a special stain to detect silver in tissue?  If there is please share 
the procedure.
Thanks,
Fanny
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[Histonet] Rhodanine method for silver

[Gudrun Lang]

This procedure can be found in „theory and practice of hitological
techniques“, page 257.

 

http://books.google.at/books?hl=de

&lr=&id=Dhn2KispfdQC&oi=fnd&pg=PA233&dq=rhodanine+silver+pigment+okamoto&ots
=JxpCewSzG8&sig=uF-Hxldt-U5dtVRhTWaaACpsf7k#v=onepage&q&f=false

 

This e-book shows only the first side of the procedure, but some comments on
silver pigments.

On the next page in brief:

Method: deparaffinize, rehydrate, incubate sections in rhodanin solution
37°C, 24 hours., wash well in destilled water, mount in glycerin jelly.

Results: silver-deposits reddish brown.

Section should be examined immidiatly, and some more notes in the book.

 

Hope this helps

Gudrun

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[Histonet] 2010 Louisiana Society for Histotechnology Symposium/Conference

[Montina Van Meter]

Hello Histonetters,

  The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.


 

  Meeting location:  The Embassy Suites Hotel

   414 Constitution Ave.

   Baton Rouge, LA  70808  

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  After the cut-off date of May13, 2010, the rooms may be
reserved at that rate per availability.  The hotel will honor this rate
two days prior and two days after our meeting (please contact the
Embassy Suites Hotel for further information: 1-225-924-6566 or
1-800-Embassy).   A complimentary hotel shuttle service is provided for
those flying into Baton Rouge.  Great dining, beautiful southern
plantations and gambling boats on the Mississippi River are just a few
of the attractions in the Baton Rouge area.  Remember to ask for the
Louisiana Society for Histotechnology group when placing your
reservation.  Walk-ins are always welcome!  If you have any questions
please contact:  Tina Van Meter at 225-603-0953 or vanme...@pbrc.edu.

 

Workshops:

 

 WS #1:  FISH - IT'S NOT JUST FOR DINNER!

 Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

 OSU Medical Center

  

 WS #2:  What is the Tumor Registry?

 Cynthia Boudreaux, LPN, CTR

 Touro Infirmary

 

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

 Pam Marcum, B.S., M.S., HT, (ASCP) 

 UAMS

 

 WS #4:  Microtomy, It's About Technique!

 Mari Ann Mailhiot, BA, HT (ASCP)

 Leica Microsystems

 

 WS #5:  Boot Camp for Histotechs 

 Mari Ann Mailhiot, BS, HT (ASCP)

 Leica Microsystems

 

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

Pam Marcum, B.S., M.S., HT (ASCP),  Bonnie Whitaker, HT (ASCP) QIHC

UAMSOSU Medical
Center

  

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

Robin Simpkins, HT (ASCP)

Biocare Medical

  

 WS #8:  Detection Chemistry & Antibody Production

Tania Ewing-Finchem, HT (ASCP)

Ventana Roche

 

 

Hope to see you in June!

 

Tina

LSH Secretary

 

 

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[Histonet] RE: proper disposal of chemicals

[Smith, Allen]

Look up "Waste Hauling" in the Yellow Pages.  Ask every company listed for a 
quote.  
Make sure the ammoniacal silver nitrate is wet; when it dries, it can rearrange 
to silver azide, a powerful and touchy primary explosive.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Friday, May 07, 2010 10:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] proper disposal of chemicals

I have a fair amount of different chemicals that are expired and need to be 
disposed of.  I am looking for advice on how to properly dispose of the 
following chemicals beyond "in accordance with local, state, and federal 
guidelines":
*Gold Chloride 0.2% aq
*Schiff reagent
*Aniline Carbol Fuchsin
*Gomori Trichrome Stain
*Glycerol
*Iodine Powder
*Ammoniacal Silver Nitrate
*1N Sodium Hydroxide
Any advice would be greatly appreciated
I am located in PA
Thanks,
Allison
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RE: [Histonet] proper disposal of chemicals

[Connolly, Brett M]

We use Clean Harbors http://www.cleanharbors.com/ and let them handle
everything.

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton,
Allison
Sent: Friday, May 07, 2010 10:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] proper disposal of chemicals

I have a fair amount of different chemicals that are expired and need to
be disposed of.  I am looking for advice on how to properly dispose of
the following chemicals beyond "in accordance with local, state, and
federal guidelines":
*Gold Chloride 0.2% aq
*Schiff reagent
*Aniline Carbol Fuchsin
*Gomori Trichrome Stain
*Glycerol
*Iodine Powder
*Ammoniacal Silver Nitrate
*1N Sodium Hydroxide
Any advice would be greatly appreciated
I am located in PA
Thanks,
Allison
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[Histonet] Reference Labs

[Marian Powers]

Hi:  Is there anyone who can provide me some references/feedback on
OncoDiagnostic Laboratories?

Thanks,

M.Seay

--
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[Histonet] proper disposal of chemicals

[Hutton, Allison]

I have a fair amount of different chemicals that are expired and need to be 
disposed of.  I am looking for advice on how to properly dispose of the 
following chemicals beyond "in accordance with local, state, and federal 
guidelines":
*Gold Chloride 0.2% aq
*Schiff reagent
*Aniline Carbol Fuchsin
*Gomori Trichrome Stain
*Glycerol
*Iodine Powder
*Ammoniacal Silver Nitrate
*1N Sodium Hydroxide
Any advice would be greatly appreciated
I am located in PA
Thanks,
Allison
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[Histonet] Special stain to demonstrate Silver

[DeGuzman, Maria]

Hello Histoneters,

Is there a special stain to detect silver in tissue?  If there is please share 
the procedure.

Thanks,

Fanny


Maria V. De Guzman| Histology Technician I

Main 908.947.1100  Fax   908.947.1085

Direct   908.947.1482 Email   mdeguz...@lifecell.com

Mobile  732.688.1386  www.Lifecell.com



LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Thursday, May 06, 2010 2:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 78, Issue 7

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Today's Topics:

   1. clo test (Tench, Bill)
   2. IDEA FOR A NEW RECYCLER (Madary, Joseph)
   3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz)
   4. CD 133 (Ingles Claire )
   5. Disposable blade holder - amateur microscopist lookingfor a
  cheap one! (Gordon Brown)
   6. Disposable blade holder - amateur microscopist lookingfor a
  cheap one! (Gordon Brown)
   7. NSH Region II Meeting-discount hotel rate deadline is coming
  up! (Goodwin, Diana)
   8. dehydration of hydrated slide (Eva Permaul)
   9. RE: dehydration of hydrated slide (Sebree Linda A)
  10. RE: dehydration of hydrated slide (Mauger, Joanne)
  11. B5 fixative (histot...@imagesbyhopper.com)
  12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED))
  13. RE: Responses to IHC CAP Validation question
  (tonia.richm...@gracepathology.com)
  14. Starting up new lab (Shaw, Sharon)
  15. Formalin fixation time for breast specimens (Richard Cartun)
  16. RE: Responses to IHC CAP Validation question
  (bsulli...@shorememorial.org)
  17. Re: myocyte damage (Merced M Leiker)


--

Message: 1
Date: Wed, 5 May 2010 13:57:20 -0700
From: "Tench, Bill" 
Subject: [Histonet] clo test
To: histonet@lists.utsouthwestern.edu
Message-ID: <2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local>
Content-Type: text/plain; charset=us-ascii

The Clo test is a clinical lab test.  You need to go to that part of the
CPT coding book (sorry I don't have it available).  88300 is an anatomic
code (gross only, ie, it requires examination of a piece of tissue or
foreign body) and is entirely inappropriate for this test.

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
bill.te...@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, May 05, 2010 1:36 PM
To: histonet@lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 78, Issue 6




Hi everyone,

I am looking to see what CPT code everyone is using for reading Clo
Tests in the pathology department. I have heard of using 87081 but I am
not sure if this is accurate as this is for culture and the CLO is
biochemical reaction not a culture.  Currently I have been using 88300
gross only.
Any help would be appreciated.

Thank you,
Amy Farnan

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Message: 2
Date: Wed, 5 May 2010 17:22:22 -0400
From: "Madary, Joseph" 
Subject: [Histonet] IDEA FOR A NEW RECYCLER
To: 
Message-ID:
<29a3cb81288e6f4ba2c9b3c8015a9a1301a37...@md1ev002.medimmune.com>
Content-Type: text/plain;   charset="us-ascii"

Some manufacturer should find a way where you can add all of the
chemicals together in one container and when the recycler runs it spits
out formalin