[Histonet] Eosin in Processor and iHC

[sgoebel]

   Has  anyone  had  any  trouble prestaining tissues in eosin and then
doing  IHC?   I'm  making  cell  blocks from clear fluid full of cells
   embe=  dded  in agar before processing and I just was wanting a way to
   be  able  to s= ee it.  Does anyone see a problem with a drop of eosin
   in the cells be= fore processing?  I don't just didn't know if someone
   else saw an issu= e?  Hope everyone has a great weekend!!

   =)

   <= br>

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   XBiotech= USA Inc.
   8201 East Riverside Dr. Bl= dg 4 Suite 100
   Austin, Texas  7= 8744
   (512)386-5107
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histonet@lists.utsouthwestern.edu

[Montina Van Meter]

Hello Histonetters,

 

  The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.

 

  Meeting location:  The Embassy Suites Hotel

 

   414 Constitution Ave.

 

   Baton Rouge, LA  70808  

 

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date for reservations has been extended to May 20,
2010.   Following that date the rooms may be reserved at that rate per
availability.  The hotel will honor this rate two days prior and two
days after our meeting (please contact the Embassy Suites Hotel for
further information: 1-225-924-6566 or 1-800-Embassy).   A complimentary
hotel shuttle service is provided for those flying into Baton Rouge.

Great dining, beautiful southern plantations and gambling boats on the
Mississippi River are just a few of the attractions in the Baton Rouge
area.  Remember to ask for the Louisiana Society for Histotechnology
group when placing your reservation.  Walk-ins are always welcome!  If
you have any questions please contact:  Tina Van Meter at 225-603-0953
or vanme...@pbrc.edu.

 

 

Workshops:

 

 WS #1:  FISH - IT'S NOT JUST FOR DINNER!

 

 Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

 OSU Medical Center

 

  

 

 WS #2:  What is the Tumor Registry?

 

 Cynthia Boudreaux, LPN, CTR

 Touro Infirmary

 

 

 

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

 

 Pam Marcum, B.S., M.S., HT, (ASCP) 

 UAMS

 

 

 

 WS #4:  Microtomy, It's About Technique!

 

 Mari Ann Mailhiot, BA, HT (ASCP)

 Leica Microsystems

 

 

 

 WS #5:  Boot Camp for Histotechs 

 

 Mari Ann Mailhiot, BS, HT (ASCP)

 Leica Microsystems

 

 

 

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

 

Pam Marcum, B.S., M.S., HT (ASCP), 

UAMS

 

Bonnie Whitaker, HT (ASCP) QIHC

OSU Medical Center

 

  

 

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

 

Robin Simpkins, HT (ASCP) 

Biocare Medical

 

  

 

  WS #8:  Detection Chemistry & Antibody Production

 

Tania Ewing-Finchem, HT (ASCP)


Ventana Roche

 

 

 

Hope to see you in June!

 

 

Tina

 

LSH Secretary

 

 

 

 

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Re: [Histonet] afb contamination

[Jackie M O'Connor]

You can eliminate the crossover contamination by performing the stain 
horizontally on a staining rack.  A lot of labs re-use the carbol fuchsin, 
which can have lots of extraneous AFB floating around, waiting patiently 
to attach itself to an unsuspecting patient slide.  Using the staining 
rack, you don't use a lot of reagent, and you never cross-contaminate.




From:
"Tench, Bill" 
To:
histonet@lists.utsouthwestern.edu
Date:
05/21/2010 02:34 PM
Subject:
[Histonet] afb contamination
Sent by:
histonet-boun...@lists.utsouthwestern.edu



We had a problem with contamination on our AFB stains, and we discovered
that it was the control slide flaking off in the copland jar which was
being used for staining the control and target slide at the same time
(makes sense as a real "control').  We identified these contaminants
because they were frequently large clusters (by "large" I would say 4-8
organisms) which we almost never see in real cases, and fortunately,
they were also not in the same plane of focus (but that can be subtle).
they did create problems.  Our solution was to stain the control
separately from the case.  No more problems. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

bill.te...@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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[Histonet] afb contamination

[Tench, Bill]

We had a problem with contamination on our AFB stains, and we discovered
that it was the control slide flaking off in the copland jar which was
being used for staining the control and target slide at the same time
(makes sense as a real "control').  We identified these contaminants
because they were frequently large clusters (by "large" I would say 4-8
organisms) which we almost never see in real cases, and fortunately,
they were also not in the same plane of focus (but that can be subtle).
they did create problems.  Our solution was to stain the control
separately from the case.  No more problems. 

 

Bill Tench

Associate Dir. Laboratory Services

Chief, Cytology Services

Palomar Medical Center

555 E. Valley Parkway

Escondido, California  92025

bill.te...@pph.org

Voice: 760- 739-3037

Fax: 760-739-2604

 


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which it is addressed, and may contain information that is privileged, 
confidential, or otherwise protected from disclosure.  Dissemination, 
distribution, or copying of this e-mail or the information herein by anyone 
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[Histonet] Polymers

[sgoebel]

   Has  anyone  ever  used  Golden  Bridge  International before?  I = am
   trying  to  find  a  secondary  polymer  (HRP)  for mouse tissue and a
   primary = antibody from Rat.  I don't want the cross linking of murine
   proteins.=  This  company  has  a  polymer, but I have never used them
   before.  Any f= eedback or other suggestions?

   Sarah Goebel, = B.A., HT (ASCP)

   Histotechnician
   <= /span>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100
   Austin, Texas  78744
   (512)386-5107
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[Histonet] Immunostaining for West Nile virus in old paraffin blocks

[Douglas Gregg]

I have developed two avidin-biotin alkaline phosphatase stains for
West Nile virus in tissues that are formalin fixed and paraffin
embedded. One uses a polyclonal and the other a monoclonal (7H2)
antibody. Both work fine in positive control chicken embryo heart
tissue. I have had difficulty in finding antigen in West Nile infected
brain tissues collected one year ago even though there is severe
encephalitis present.  It is possible that the tissue was collected
too late in disease. It was 10-14 days post exposure. However, I am
concerned that the negative results may be  due to the age of the
tissues in paraffin rather than having been collected too late in the
disease.  I have a good retrieval method and the tests are very
reliable on acute cases that are formalin fixed for up to one month
and paraffin embedded.

Has anyone done any retrospective studies on paraffin blocks of West
Nile virus infected  tissues as old as a year or more.If so, did they
stain? Also, I would very much like to find a paraffin block that is
one year old for a control. A crow heart would be a good choice. I am
sure there must be labs out there that have done testing of crows or
other animals found dead and submitted for testing. I would like to
try my staining on one that was collected a year ago. If it stains,
then it is not likely due to the age of the tissues in paraffin.

Help would be most appreciated if anyone can spare an old paraffin
block of a known case.

Douglas Gregg DVM PhD
Veterinary Pathologist
Southold, NY

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[Histonet] RE: possible AFB contaminant

[Della Speranza, Vinnie]

Do you use DI water in your flotation baths ?

If your DI system is not already so equipped, install a 0.5 micron filter where 
you withdraw the water and be sure to change this filter monthly.

I'm willing to bet the bugs are on the slides before the stain is performed 


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
debra.or...@uchospitals.edu
Sent: Thursday, May 20, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] possible AFB contaminant

Please help, we have had an increase in demands for repeats on our AFB stain. 
Our pathologists have been noticing what they believe are "false positives" on 
our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. 
Because of this problem, we have been doing side by side comparisons using the 
Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our 
DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.
 
Thanks Debi


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notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 


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RE: [Histonet] weekend fixation

[sgoebel]

   This  may  be true today, but I heard CAP was going to set this int= o
   stone in the next couple of months?

   Sarah Goebel, B.A., HT (ASCP)<= /em>

   Histotechnician

   XBiotech USA Inc.

   <= div>

   8201 = East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744<= /span>

   (512)386-5107

    Original Message 
   Subject: RE: [Histonet] weekend fixation
   From: "Richard Cartun" <[1]rcar...@= harthosp.org>
   Date: Thu, May 20, 2010 8:07 pm
   To: <[2]linda.marg...@childr= ens.com>,<[3]mpe...@grhs.net&= gt;,
   <[4]sgoe...@xbiotech.com>
   Cc: <[5]histo...@lists= .utsouthwestern.edu>
   Dear Colleagues:

   Pleasekeep   in   mind   that=   the   ASCO/CAP   guidelines   are
   "recommendations",  not mandates.  You can=  experiment, validate, and
   establish  your own formalin-fixation p= rotocol for breast specimens.
   At  Hartford  Hospital  I have established= a minimum of 4.5 hours for
   needle  core  specimens.   This  includes  0.5 = hour of pre-processor
   fixation  and  4  hours of fixation on our tissue proces= sors.  We do
   use  the  6  hour minimum for all excisional specimens. = ; WE HAVE NO
   MAXIMUM  FIXATION  TIME.   I  can show you a= bsolutely beautiful HER2
   immunoreactivity  and  gene  amplification  on breast = cancer tissues
   that  have  been  fixed for 1 week, 1 month, 1 year, and l= onger.  If
   you  don't  agree with the guidelines, work with your patholo= gist(s)
   and  establish  your  own  fixation  policy.   And,  please remember,   
fixation time is only one piece to the puzzle.  In my experience, min   imizing 
 ischemic  time,  making sure that the tissue does not dry out
   prior  t= o fixation, and submitting thin (2 mm) sections are probably
   more impo= rtant than fixation time.

   Richard
   <= div style="clear: both;">Richard W. Cartun, Ph.D.
   Director, Histology = & Immunopathology
   Director, Biospecimens
   Assistant Director, Anat= omic Pathology
   Hartford Hospital
   80 Seymour Street
   Hartford, CT 06= 102
   (860) 545-1596
   (860) 545-0174 Fax
   >>> "Mike Pen= ce" <[6]mpe...@grhs.net> 05/20/10= 6:10 PM >>>
   Yeah. Good luck with that one. The minimum clearl= y states 6 hrs.
   Mike
   -Original Message-
   From: <= a
   href="mailto:histonet-boun...@lists.utsouthwestern.edu";>histonet-bou
   nce= s...@lists.utsouthwestern.edu
   [[7]mailto:histonet-boun...@lists.utsouthwestern.edu]= On Behalf Of
   [8]sgoe...@xbiotech.= com
   Sent: Thursday, May 20, 2010 3:08 PM
   To: LINDA MARGRAF
   = Cc: [9]histo...@lists.uts= outhwestern.edu
   Subject: RE: [Histonet] weekend fixation
   From what I have seen and heard you can have fixation times up to=2   hours 
and still be ok?
   Sarah Goebel, B.A., HT (ASCP)
   &= lt;=iv>
   Histo=echnician
   XBiotech USA Inc.
   82= 01 East Rivers=de Dr. Bldg 4 Suite 100
   Austin, Texas 78744
   (5= 12)386-5107
    Original Message 
   Subject: [Histon= et] weekend fixation
   From: "LINDA MARGRAF" <[10]linda.marg...@childrens.com>
   Date: Thu, M= ay 20, 2010 12:04 pm
   To: <[11]histo...@lists.utsouthwestern.edu>
   Histonetters:
   = Here's a message I was asked to post..
   Dear Colleagues,
   I have = the following question concerning tissue processing. We do
   a
   lot  o=  =  IHC work on NF fixed tissue. To standardize and minimize
   the
   effect= of NF=ixation, we fixate the tissue always for 24h. This is
   of cours= e a problem=for tissues taken on Friday. In the past, we
   asked our te= chnicians to come=n Saturday to embed the tissues in
   paraffin. Unfort= unately, this is not p=ssible anymore, and that is
   why I need your ad= vice. What would you suggest= 1) to leave the
   tissue in NF until Sund= ay evening and start processing, =r 2) to
   keep the fixation time (24 = hours) and leave the tissue in alcohol
   70% until Sunday evening and the= n start processing.
   Thanks for your advice.
   Kind regards,
   Wim.Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
   Cell biology and Histol= ogy
   Department of Morphology - Faculty of Veterinary Medicine
   Ghent= University
   Salisburylaan 133, B-9820 Merelbeke, BELGIUM
   Please con= sider the environment before printing this e-mail.
   This e-mail, facsimi= le, or letter and any files or
   attachments
   transmitted =ith it co= ntains
   information that is confidential and privileged. This informatio= n
   is
   intend=d only for the
   use of the individual(s) and entit= y(ies) to whom it is addressed.
   If
   you ar= the intended
   recip= ient, further disclosures are prohibited without
   proper
   authorizati= on.=f you are not
   the intended recipient, any disclosure, copying, pr= inting, or use
   of
   this i=formation is
   strictly prohibited and= possibly a violation of federal or state
   law
   and re=ulations. If= you
   have received this information in error, please notify
   Childre= n's
   Medical C=nter Dallas
   immediately at 214-456- or via e

[Histonet] HOT JOB! Histology Supervisor needed in Dallas, TX

[Betsy Hoffman]

Hi..

Thought you'd be interested in seeing this HOT Histotech job opportunity 
currently available through CompHealth.  If you want to find out more...contact 
me!!

Histology Supervisor needed in Dallas, TX
This leading anatomic pathology practice located in Dallas, Texas is seeking an 
experienced Histology Supervisor to cover their overnight shift.   If you have 
a BS degree in Biological/Physical sciences or equivalent combination of 
education and experience; HT (ASCP) or HTL (ASCP) certification;  and 3-6 years 
Histology experience, with 1-2 years in a leadership role we want to talk to 
you!
Responsible for the day-to-day operations of the Histology laboratory and 
supervision of
the technical and support staff. In conjunction with the Department Manager, 
ensures that
all departmental policies and procedures meet the standards of current state 
and federal
regulations.
Night Shift: 10p - 7a
**NO TRAVELERS PLEASE!**


**Who do you know??
If this doesn't appeal to you...maybe you know someone else who's 
lookingrefer them to me and we'll pay you a referral fee.

Betsy Hoffman
Search Consultant, Lab Sciences
CompHealth Permanent Placement
6451 North Federal Highway, Suite 702
Ft. Lauderdale, FL 33308
Direct: 1-954-837-2622| Office:  1-866-782-9029, X2622| Fax: 1-800-420-2329
betsy.hoff...@comphealth.com


Search Jobs Online | Visit us at  www.comphealth.com

Learn more about our award-winning company and the people behind it!


ASK ABOUT OUR $250 BONUS FOR REFERRALS!!!

Customer service is the key to success.
Are you satisfied with your experience?  Send your comments to my manager at  
carlos.hag...@comphealth.com


This is a commercial email from CompHealth.  If you do not want to receive 
future emails from CompHealth, please reply to the sender of this email and ask 
to be removed.





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[Histonet] recommendation for C-kit antibody

[Jennifer Campbell]

I'm looking for a C-kit antibody for IHC on FFPE K9 and Fel tissue.
I've only found a couple of vendors that meet those requirements.  Can
anyone recommend one to me?
 
Thanks,
 
Jennifer Campbell
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RE: [Histonet] Nissl Stain P0 Mouse Head

[sgoebel]

   Did you decal the skull?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician

   XBiotech USA Inc.

   8201 Eas= t Riverside Dr. Bldg 4 Suite 100

   = Austin, Texas  78744

   (512)386-5107

    Original Message 
   Subject: [Histonet] Nissl Stain P0 Mouse Head
   From: Kevin Gillinder <[1]= k.r.gillin...@newcastle.ac.uk>
   Date: Fri, May 21, 2010 5:00 am
   To: "Histonet@lists.utsouthwestern.edu"
   <[2]histo...@lists.uts= outhwestern.edu>
   Hi,
   I  am  having  some  trouble  with  Nissl  staining newborn (P0) mouse
   heads/hindb= rain.
   The  nissl  stain  appears  very  faint  in the centre of my sections.
   (Paraffin = embedded, 7um thick)
   Is this likely to be a penetration issue?
   I fixed in 4% PFA O/N before processing - Would that affect it?
   I  left the skull intact, but removed the skin - should I have removed
   some = of the skull?
   Any help appreciated!!
   Regards,
   --
   Kevin
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References

   1. 3D"mailto:k.r.gillin...@newcastle.ac.uk";
   2. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   3. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   4. 
3D"http://lists.utsouthwestern.edu/mailman/listin___
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[Histonet] High Paying Histology Jobs

[Alisha Dynan]

Dear Histonet Members,









I hope you are doing well. I am a Recruiter at a highly successful and well 
respected Healthcare recruiting firm.  I help place Lab Professionals in 
permanent positions across the country and I wanted to see if you are 
interested in exploring other career opportunities?  We are completely free of 
charge to candidates and and we work on quite a few laboratory openings across 
the country. Our clients typically assist with relocation expenses. 





Great Histotech Day Shift Job:


I am currently working with a client in rural Central New York in Otsego 
County.  This teaching hospital has a very strong academic program and 
affiliation with one the top universities in the state. My client is currently 
expanding their laboratory and looking for qualified Histotechnologists with 
valid NYS license (If you do not have one, please let me know and I can assist 
you in apply for one). My client is looking for candidates for 2 day shift 
positions that he has available. This hospital system offers a very competitive 
base salary/hourly rate, shift differentials, an outstanding benefits and 
retirement package, and relocation assistance, if needed. 

Great Histology Supervisor Day Shift Job:



I am currently working with a client in central Georgia.  This 500+ bed 
teaching hospital has a very strong academic program.  My client is currently 
looking for a qualified Histology Supervisor, with at least 5 years experience 
in histology and preferably experience as a supervisor or lead technologist. 
This hospital system offers a very competitive base salary/hourly rate, shift 
differentials, an outstanding benefits and retirement package, and relocation 
assistance, if needed. 


If you're interested in learning more about these opportunities or 
opportunities in a certain geographic location please reply with an updated 
resume and let me know when a good time to reach you is.  







If this is not the right fit for you please let me know who you can recommend 
and give me an idea of what types of positions you'd be interested in hearing 
about in the future.  I cover the entire US and have am working on Lab 
positions at all levels. We offer a very generous referral bonus for anyone you 
refer to us that we place into any position across the country.  

To view some additional opportunities please visit our website at 
www.ka-recruiting.com.  




















Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

ali...@ka-recruiting.com

www.ka-recruiting.com

 






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[Histonet] DOG1 (c-kit neg. GIST tissue)

[Steven Wilkes]

Hello

We are interested in validating DOG1, but while the literature states 5-15%
of GIST cases are c-kit negative, we are unable to obtain any.  We currently
use CD117, but would add DOG1 to our library if we could validate that it is
a more sensitive marker - and to do so we'd need c-kit negative GIST
tissue.  Does anyone have a c-kit negaitve GIST case on hand or know of a
company that sells such tissue?  Thanks in advance

Steven
srwil...@gmail.com
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[Histonet] Oil Red O Staining

[Willman, Sharon]

Hi,
Other than using plus slides and air drying, what other techniques do you use 
to help frozen section tissues for Oil Red O to adhere to the slides?  How long 
do you usually air dry slides for cryostat sectioning?
Thanks in advance for your input.
Sharon



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[Histonet] Nissl Stain P0 Mouse Head

[Kevin Gillinder]

Hi,

I am having some trouble with Nissl staining newborn (P0) mouse heads/hindbrain.
The nissl stain appears very faint in the centre of my sections. (Paraffin 
embedded, 7um thick)

Is this likely to be a penetration issue?
I fixed in 4% PFA  O/N before processing - Would that affect it?
I left the skull intact, but removed the skin - should I have removed some of 
the skull?

Any help appreciated!!

Regards,

--
Kevin

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Re: [Histonet] possible AFB contaminant

[BSullivan]

My first question would be what are you using for  a control? I know this
sounds strange but I have, in the past, had a problem with commercially
made ones. The AFB actually came away from the material and applied itself
to the patient slide. Crazy huh? This caused a real mess until we figured
it out and changed vendors. Also if you are doing them by hand you need to
filter after each use. Hope this helps. There is a definate difference in
appearance between a patient positive result and a loose organism. Hope
this helps.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


   
   
 Sent by:   To 
 histonet-bounces@  
 lists.utsouthwest  cc 
 ern.edu   
   Subject 
   [Histonet] possible AFB contaminant 
 05/20/2010 03:41  
 PM
   
   
   
   




Please help, we have had an increase in demands for repeats on our AFB
stain. Our pathologists have been noticing what they believe are "false
positives" on our slides. We perform our AFBs on the Ventana Nexus and some
are done by hand. Because of this problem, we have been doing side by side
comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested
our DI water sytem and have different techs cutting using different
waterbaths.
Any advice or comments would be greatly appreciated.

Thanks Debi



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University of Chicago Medical Center



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