[Histonet] I will be out of office beginning the afternoon of 6/3/2010 returning 3/24/2010

[Marilyn . A . Weiss]

I will be out of the office starting  06/03/2010 and will not return until
06/07/2010.

.In my absence please ask for Mary Campbell .  If this is urgent you can
contact me on my cell phone number 858-472-4266.
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Re: [Histonet] H&E Stainer Validations

[Rene J Buesa]

Albert:
In histology there are only very few standards. That is why CAP has a check 
list when inspecting histolabs.
If you are doing a "validation", get as many different tissue sections as you 
can. Stain them "as usual" (namely using the method you are trying to 
substitute).
Stain identical sections with your method or instruyment. Label them just by 
numbers, and give them to the pathologist for a blind evaluation.
They should answer just one questions: is either section within each pair 
better than the other (telling which one) or are they equally useful for 
diagnosis.
The results analyze with Chi a square test where the "theoretical" frequency is 
P=0.50.
As "simple" as that.
René J. 

--- On Fri, 6/4/10, Santiago, Albert  wrote:


From: Santiago, Albert 
Subject: [Histonet] H&E Stainer Validations
To: histonet@lists.utsouthwestern.edu
Date: Friday, June 4, 2010, 11:30 AM


Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was
wondering if anyone knows if there's a standard H&E Validation protocol
or if it just varies from lab to lab? Thanks for your help :-)

Albert Santiago, HT(ASCP)
Laboratory Manager
Dermatopathology
215-662-6759/6539-office
215-520-3797-BB
215-662-7520-fax
We will be relocating, as of 6/14/2010 
our lab will be located
at 3020 Market St. Ste. 201



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Re: [Histonet] PARAPLAST QUALITY

[Langenberg, Stacey]

We use paraplast Xtra supplied from Leica\Surgipath and have seen no problems 
whatsoever.
Stacey Langenberg
Sent via BlackBerry from T-Mobile

-Original Message-
From: Karen Kay 
Date: Fri, 4 Jun 2010 11:43:05
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] PARAPLAST QUALITY

Good Morning,
Our Paraplast has also been produced by McCormick and to this point have not 
seen any issues.  However it is my understanding that Leica(Surgipath) has 
taken over as supplier and producer of the Paraplast (trademark).   This 
information was relayed to us by our Surgipath representative quite a few 
months ago.
This past week, I received a phone call from a different Lab supplier 
indicating to me that there have been issues cropping up with the Paraplast 
from the new manufacturer.
I did do a search on the Histonet and have not found messages to that effect.  
It would be interesting and proactive to find out if there indeed have been 
issues  cropping up recently with the Paraplast.
Thanks
Karen J Kay, MLT
Supervisor - Histopathology and Cytology Laboratory
Chinook Regional Hospital-South Zone West - Alberta Health Services
Lethbridge, Alberta, Canada
karen@albertahealthservices.ca


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: June 4, 2010 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 79, Issue 4

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. outside review. (Tench, Bill)
   2. Laryngotracheitis  (Charles, Roger)
   3. Paraplast (Rebecca Johnson)
   4. RE: Paraplast (Baez, Janet)
   5. Lab Manager Job Opportunity in San Francisco
  (adam.t.anth...@kp.org)
   6. coverslippers (Gale Limron)
   7. RE: coverslippers (Horn, Hazel V)
   8. INI-1 (Horn, Hazel V)
   9. Re: Paraplast (dkb...@chs.net)
  10. AW: INI-1 (Kappeler, Andreas (PATHOLOGY))
  11. RE: INI-1 (McMahon, Loralee A)
  12. Unsubscribe. (McCormick, James)
  13. H&E Stainer Validations (Santiago, Albert)
  14. Re: Unsubscribe. (Emily Sours)


--

Message: 1
Date: Thu, 3 Jun 2010 10:49:45 -0700
From: "Tench, Bill" 
Subject: [Histonet] outside review.
To: histonet@lists.utsouthwestern.edu
Message-ID: <2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local>
Content-Type: text/plain; charset=us-ascii

I thought I sent this yesterday, but it didn't address properly.  I guess 
things are different in my part of the world in terms of the frequency of this 
activity compared with others.  Please see the rest.

The policy/procedure requiring review of outside slides before definitive 
treatment (including surgery, radiation therapy, or
chemotherapy) in another institution is a fairly common but frequently 
unenforced policy in many hospitals other than academic institutions.
The policy may reside in the Pathology Dept, Surgery Dept, or more frequently 
(and safely) as a standard Hospital policy.  Typically, enforceability is 
improved if the policy is a Hospital policy.  There is an ever growing body of 
evidence that this policy benefits the patient, the hospital, the clinician, 
and the pathologist.  Compliance is always an issue, especially outside of 
academic centers, who, out of a belief that the community pathologists are 
prone to mistakes that academic centers never make, insist on review before 
treatment.  "Everyone's benefit" is always a nice "carrot," but unfortunately, 
without a "stick," compliance may be a challenge.  For the surgeons, at least, 
the risk of cancellation of the case if a TIMELY request for outside review has 
not been made serves as a useful "stick," the application of which should be 
applied judiciously (and perhaps with a flack jacket).
Radiation and Chemo cases require either: 1) cooperation of these departments  
or 2) external review of all cases treated in those departments to determine 
whether or not case review has occurred.  The goal is to, as best as possible, 
confirm the diagnosis as well as other aspects that may have an impact on the 
appropriate therapy, and to do so without significant delay in the patient's 
care.  Anything that makes the process easier is a good idea.  In my system, 
the responsibility of the treating physician is to fax to the pathology dept a 
copy of the report when they have decided to proceed with additional treatment 
(surgery, r

[Histonet] PARAPLAST QUALITY

[Karen Kay]

Good Morning,
Our Paraplast has also been produced by McCormick and to this point have not 
seen any issues.  However it is my understanding that Leica(Surgipath) has 
taken over as supplier and producer of the Paraplast (trademark).   This 
information was relayed to us by our Surgipath representative quite a few 
months ago.
This past week, I received a phone call from a different Lab supplier 
indicating to me that there have been issues cropping up with the Paraplast 
from the new manufacturer.
I did do a search on the Histonet and have not found messages to that effect.  
It would be interesting and proactive to find out if there indeed have been 
issues  cropping up recently with the Paraplast.
Thanks
Karen J Kay, MLT
Supervisor - Histopathology and Cytology Laboratory
Chinook Regional Hospital-South Zone West - Alberta Health Services
Lethbridge, Alberta, Canada
karen@albertahealthservices.ca


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: June 4, 2010 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 79, Issue 4

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. outside review. (Tench, Bill)
   2. Laryngotracheitis  (Charles, Roger)
   3. Paraplast (Rebecca Johnson)
   4. RE: Paraplast (Baez, Janet)
   5. Lab Manager Job Opportunity in San Francisco
  (adam.t.anth...@kp.org)
   6. coverslippers (Gale Limron)
   7. RE: coverslippers (Horn, Hazel V)
   8. INI-1 (Horn, Hazel V)
   9. Re: Paraplast (dkb...@chs.net)
  10. AW: INI-1 (Kappeler, Andreas (PATHOLOGY))
  11. RE: INI-1 (McMahon, Loralee A)
  12. Unsubscribe. (McCormick, James)
  13. H&E Stainer Validations (Santiago, Albert)
  14. Re: Unsubscribe. (Emily Sours)


--

Message: 1
Date: Thu, 3 Jun 2010 10:49:45 -0700
From: "Tench, Bill" 
Subject: [Histonet] outside review.
To: histonet@lists.utsouthwestern.edu
Message-ID: <2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local>
Content-Type: text/plain; charset=us-ascii

I thought I sent this yesterday, but it didn't address properly.  I guess 
things are different in my part of the world in terms of the frequency of this 
activity compared with others.  Please see the rest.

The policy/procedure requiring review of outside slides before definitive 
treatment (including surgery, radiation therapy, or
chemotherapy) in another institution is a fairly common but frequently 
unenforced policy in many hospitals other than academic institutions.
The policy may reside in the Pathology Dept, Surgery Dept, or more frequently 
(and safely) as a standard Hospital policy.  Typically, enforceability is 
improved if the policy is a Hospital policy.  There is an ever growing body of 
evidence that this policy benefits the patient, the hospital, the clinician, 
and the pathologist.  Compliance is always an issue, especially outside of 
academic centers, who, out of a belief that the community pathologists are 
prone to mistakes that academic centers never make, insist on review before 
treatment.  "Everyone's benefit" is always a nice "carrot," but unfortunately, 
without a "stick," compliance may be a challenge.  For the surgeons, at least, 
the risk of cancellation of the case if a TIMELY request for outside review has 
not been made serves as a useful "stick," the application of which should be 
applied judiciously (and perhaps with a flack jacket).
Radiation and Chemo cases require either: 1) cooperation of these departments  
or 2) external review of all cases treated in those departments to determine 
whether or not case review has occurred.  The goal is to, as best as possible, 
confirm the diagnosis as well as other aspects that may have an impact on the 
appropriate therapy, and to do so without significant delay in the patient's 
care.  Anything that makes the process easier is a good idea.  In my system, 
the responsibility of the treating physician is to fax to the pathology dept a 
copy of the report when they have decided to proceed with additional treatment 
(surgery, rads, etc), and the pathology dept does all the work associated with 
requesting the slides and insuring a timely review.  We advise (and 
continuously train) the outside office staff that they must provide us this 
information ASAP because some outside institutions are woefully slow in sending 
th

[Histonet] Control Blocks

[Knutson, Deanne]

Hello,

Does anyone out there have positive Pneumocystis control blocks that they may 
want to trade with?  We have some positive GRAM control blocks that we could 
exchange.  Please let me know.  Thank you so much!

Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
 (701)-530-6730
dknut...@primecare.org



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Re: [Histonet] Unsubscribe.

[Emily Sours]

No.

Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose


On Fri, Jun 4, 2010 at 11:03 AM, McCormick, James wrote:

> Please unsubscribe my email from your list.
>
> Thank you,
> James B. McCormick, M.D.
>
> 
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> delete it from your system. Any review, dissemination, distribution, or
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> and may be subject to legal restriction.
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[Histonet] H&E Stainer Validations

[Santiago, Albert]

Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was
wondering if anyone knows if there's a standard H&E Validation protocol
or if it just varies from lab to lab? Thanks for your help :-)

Albert Santiago, HT(ASCP)
Laboratory Manager
Dermatopathology
215-662-6759/6539-office
215-520-3797-BB
215-662-7520-fax
We will be relocating, as of 6/14/2010 
our lab will be located
at 3020 Market St. Ste. 201



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[Histonet] Unsubscribe.

[McCormick, James]

Please unsubscribe my email from your list.

Thank you,
James B. McCormick, M.D.


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reproduction of this message by unintended recipients is strictly prohibited 
and may be subject to legal restriction.


Thank you for your cooperation.
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[Histonet] RE: INI-1

[McMahon, Loralee A]

We have been using an INI-1 from Cell Marque.  With a 1/50 dilution and a low 
pH heat retrieval.  Although I accidently had a slide go through the higher pH 
heat retrieval and I didn't see a big difference in the staining.  
I use the Dako system so it was a 30 minute incubation with a linker added.  
Hope that helps. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V 
[hor...@archildrens.org]
Sent: Friday, June 04, 2010 7:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] INI-1

We are having problems with this immunostain.  If your lab is
successfully using this antibody will you please help me out?

Where do you purchase your antibody?  Is it RTU?  What type of antigen
retrieval?  Incubation time?

Thanks.



Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's WaySlot 820

Little Rock, AR   72202



phone   501.364.4240

fax501.364.3155



visit us on the web at:www.archildrens.org



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[Histonet] AW: INI-1

[Kappeler, Andreas (PATHOLOGY)]

Hi Hazel

We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg 
Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100 
mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5. 
Incubation at RT, 60 min. Visualization with polymer-based HRP system/DAB. Hope 
this helps.

Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

P.S. when asking about advice on antibodies, try to be as specific as possible: 
clone, Ig-conc and/or dilution, retrieval protocols tried, visualization 
systems tried. Helps o narrow down possilbe problems.


Von: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] im Auftrag von Horn, Hazel V 
[hor...@archildrens.org]
Gesendet: Freitag, 4. Juni 2010 13:55
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] INI-1

We are having problems with this immunostain.  If your lab is
successfully using this antibody will you please help me out?

Where do you purchase your antibody?  Is it RTU?  What type of antigen
retrieval?  Incubation time?

Thanks.



Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's WaySlot 820

Little Rock, AR   72202



phone   501.364.4240

fax501.364.3155



visit us on the web at:www.archildrens.org



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Re: [Histonet] Paraplast

[DKBoyd]

We use Richard Allen, Type 6 for both impregnation and embedding.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







"Rebecca Johnson"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
06/03/2010 04:42 PM

To
"histonet" 
cc

Subject
[Histonet] Paraplast






What paraffin is everyone using. We have been a Paraplast user made by 
McCormick.  We have noticed black to gray small dusty particles in the 
embedding well. I have tried a couple others, but the ribbons compress.  I 

have tried the a different angle at 3 with no difference.  I would 
appreciate some help from all of you.
Thanks
Becky 



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[Histonet] INI-1

[Horn, Hazel V]

We are having problems with this immunostain.  If your lab is
successfully using this antibody will you please help me out?

Where do you purchase your antibody?  Is it RTU?  What type of antigen
retrieval?  Incubation time?  

Thanks.

 

Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's WaySlot 820

Little Rock, AR   72202

 

phone   501.364.4240

fax501.364.3155

 

visit us on the web at:www.archildrens.org

 

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dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
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RE: [Histonet] coverslippers

[Horn, Hazel V]

We love our CV5030 glass coverslipper.  It integrates with their stainer
for a seamless flow.   It's so nice to put the slides on to stain and
have them coverslipped when they come off.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax501.364.3155
 
visit us on the web at:www.archildrens.org
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale
Limron
Sent: Friday, June 04, 2010 5:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] coverslippers

 We are currently evaluating coverslippers and I'd appreciate any
info/opinions on the following:
Hacker HCM 6000
TBS SHUR/Mount
Thermo Scientific (Shandon) ClearVue
Tissue-Tek Glas g2
Leica CV5030
 We will be looking at stainers within a couple of years so this is
something we need to consider also...
Thanks,
Gale


Gale Limron
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext. 2562



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[Histonet] coverslippers

[Gale Limron]

 We are currently evaluating coverslippers and I'd appreciate any info/opinions 
on the following:
Hacker HCM 6000
TBS SHUR/Mount
Thermo Scientific (Shandon) ClearVue
Tissue-Tek Glas g2
Leica CV5030
 We will be looking at stainers within a couple of years so this is something 
we need to consider also...
Thanks,
Gale


Gale Limron
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext. 2562



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without copying and kindly e-mail a reply to inform us of the mistake in 
delivery.
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