Re: [Histonet] Oncotech

[Brandi Higgins]

Hello,

Many of our surgeons who previously requested we send specimens to Oncotech
switched to Precision Therapeutics about a year or so ago (although some
still used Oncotech at times).  We have used Precision with no problems.
They even supplied us with a refrigerator to store the specimen collection
kits which need to be refrigerated, and our reps have been very helpful
overall.  Hope this helps.

Brandi Higgins, HT(ASCP)
On Mon, Jun 7, 2010 at 9:27 AM, Senn, Amy R  wrote:

> Hello Histonet,
>
>
>
>
>
> Now that Oncotech is out of business, who is everyone sending their
> extreme resistance assays to?
>
>
>
>
>
> Thanks,
>
> Amy, HT
>
> Camp Hill, PA
>
>
>
>
>
>
>
>
>
>
>
> Attention:  This Message is intended only for the use of the individual or
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Re: RE: [Histonet] block disposal

[Steven Hacker]

   Hey, Joyce...

   Contact  some  of  your  vendors...Many  of  them  would  love  to get
   addition=  al  blocks to run assays, use as controls...etc, etc...Some
   companies  will d= onate to laboratory/facility sponsored charities to
   compensate/donate  to  th=  ose  causes...It  can  make  a significant
   difference in future assays.



   Regards,

   Steven Hacker



   Ps...Yes,  I  work  for Biocare Medical and we're always interested in
   bl=  ocks  for  our  'library'...Oh also, you should know vendors only
   prefer  to kn= ow what specimen type it is, what assays were run on it
   and   if   they   were  po=  sitive/negative...No  HIPPA  compromising
   information will ever be accepted.<= /DIV>
   Jun 8, 2010 01:38:11 PM, jsta...@masshistology.com wrote:
   <= /DIV>

 We  are  also  s=  trictly  research  and  avoid  this situation by
 returning all
 blocks back t= o the clients.
 ___
 James E. Staruk HT(ASCP)www.masshistology.com
 www.nehorselabs.com
 -Orig= inal Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [ma=  ilto:histonet-boun...@lists.utsouthwestern.edu]  On Behalf Of
 Sherwood,
 M= argaret
 Sent: Tuesday, June 08, 2010 11:36 AM
 To: Joyce Cline; histo= n...@lists.utsouthwestern.edu
 Subject: RE: [Histonet] block disposal
 <= BR>
 We  are  a  research laboratory. Our policy, based on communication
 via= the
 Histonet,  is  to keep 2 years worth of paraffin blocks in the lab.
 B= locks
 greater than 2 years old up to 10 years old, we send to off-site s 
torage.
 Blocks  greater  than 10 years old are destroyed by the storage co 
mpany since
 they have them.
 When  we first set up this system last= year, we had 20 years worth
 of blocks
 and the majority were put in our = red hazardous waste bucket to be
 incinerated.
 Peggy
 = -Original Message-
 From: histonet-boun...@lists.utsouthwestern.e= du
 [mailto:histonet-boun...@lists.utsouthwestern.edu]   On  Behalf  Of
 Joyce= Cline
 Sent: Tuesday, June 08, 2010 10:28 AM
 To: histo...@lists.utsou= thwestern.edu
 Subject: [Histonet] block disposal
 How  does  everyon= e handle disposing of blocks that are more than
 10 years
 old?  Are  they  i=  ncinerated,  hauled away by a waste management
 company or just
 thrown in = the trash.
 In  Maryland I was told they can't be thrown in the regula= r trash
 by a state
 waste official.
 Joyce Cline, Technical Special= ist
 Hagerstown Medical Laboratory
 301-665-4980
 fax 301-665-4941 jo= yce.cl...@wchsys.org
 
 *   CONFIDENT=   IALITY  NOTICE  *  This  message  contains
 confidential
 information and i= s intended only for the individual named. If you
 are not
 the  named  addre=  ssee  you should not disseminate, distribute or
 copy this
 e-mail.  Please  =  notify  the sender immediately by e-mail if you
 have received
 this e-mail= by mistake and delete this e-mail from your system.
 ___= 
 Histonet mailing list
 histo...@lists.uts= outhwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histone= t
 The  information in this e-mail is intended only for the person= to
 whom it is
 addressed.  If  you  believe this e-mail was sent to you in = error
 and the
 e-mail  contains patient information, please contact the Pa= rtners
 Compliance
 HelpLine  at  http://www.partners.org/complianceline  .  I=  f  the
 e-mail was sent
 to  you in error but does not contain patient infor= mation, please
 contact the
 sender and properly dispose of the e-mail.
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 Checked by AVG - www.avg.com
 Versi=  on:  9.0.829 / Virus Database: 271.1.1/2916 - Release Date:
 06/08/10
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[Histonet] Oncotech

[Marc & Sandy Shaeffer]

Amy Senn asked:

Now that Oncotech is out of business, who is everyone sending their
extreme resistance assays to?

We were just briefed today by a company called Precision Therapeutics 
out of Pittsburgh PA that they perform Chemo sensitivity and resistance 
tests and the test is called ChemoFx.  Their website is 
www.precisiontherapeutics.com.  I have no affiliation with this company.


Precision Therapeutics supplies the shipping container and fluid, 
usually overnight delivery with Saturday deliveries allowed.


Marc Shaeffer
Tucson Medical Center
Arizona

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[Histonet] Re: Leica Paraplast

[Debbie Dreesen]

Hi Becky,
There were several posts between March 23-31 addressing the quality of the 
Paraplast if you want to check them out. We tried trouble-shooting everything 
we could think of when we noticed a change in the paraffin. The texture was 
very different and it was so sticky our sections stuck to the blade no matter 
how we tried to fix the problem. It was gritty and caused nicks in the blade 
after just a few sections. We tried several different blades to make sure it 
wasn't a problem there or a bad batch. Different brands as well as both low and 
high profile. We tried the different blades and settings because we had just 
replaced all our microtomes and thought maybe we just needed to make some 
adjustments but we experienced the same problem with each blade at every 
setting we tried and concluded the paraffin was the problem. The issues were 
resolved after we switched back to ordering the EM-400 from Cardinal.


Debbie Dreesen, HT(ASCP)



Message: 3
Date: Thu, 3 Jun 2010 15:20:37 -0500
From: "Rebecca Johnson" 
Subject: [Histonet] Paraplast
To: "histonet" 
Message-ID: 
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
    reply-type=original

What paraffin is everyone using. We have been a Paraplast user made by 
McCormick.  We have noticed black to gray small dusty particles in the 
embedding well. I have tried a couple others, but the ribbons compress.  I 
have tried the a different angle at 3 with no difference.  I would 
appreciate some help from all of you.
Thanks
Becky 

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[Histonet] Re: Block Disposal

[Debbie Dreesen]

Joyce,
We disposed of of tissues and their containers after 6 weeks on the shelf. 
Outdated blocks were placed in the same totes used for the tissues and picked 
up by an outside company for incineration.

Debbie Dreesen, HT(ASCP)



Message: 9
Date: Tue, 8 Jun 2010 10:27:55 -0400
From: Joyce Cline 
Subject: [Histonet] block disposal
To: "histonet@lists.utsouthwestern.edu"
    
Message-ID:
    
Content-Type: text/plain; charset="iso-8859-1"

How does everyone handle disposing of blocks that are more than 10 years old? 
Are they incinerated, hauled away by a waste management company or just thrown 
in the trash.

In Maryland I was told they can't be thrown in the regular trash by a state 
waste official.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cl...@wchsys.orgmailto:joyce.cl...@wchsys.org

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[Histonet] RE: Refrigerator Mold

[Weems, Joyce]

oops = didn't intend to hit send..

But perhaps try dessicant or baking soda, which would reduce the moisture

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson 
[jander...@halozyme.com]
Sent: Tuesday, June 08, 2010 3:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks 
of folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that 
seems to have worked, but I'm not sure if that is detrimental to slide and 
reagent storage.
I have purchased some strong disinfectants also, but I don't know if those 
vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
jander...@halozyme.com




The information transmitted in this email is confidential and is intended only 
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[Histonet] Histology Jobs

[K.C. Carpenter]

Hello Everyone, 

 

I am one of the owners of a healthcare recruiting firm that specializes in 
placing Lab Professionals and I wanted to see if you'd be interested in 
learning about potential career advancing job opportunities? We are completely 
free of charge to candidates and can be a great resource for you in helping you 
to find your next job. Our clients offer competitive compensation packages and 
typically assist with relocation expenses. I am currently working on some great 
job opportunities in various areas across the country. 
 

One position in particular you may be interested in is a 1st shift 
Histotechnologist job located outside of Indianapolis. This opportunity is with 
a 250 bed hospital that is consistently ranked as being one of the top 10 
employers in Indiana.  My client is offering an above average compensation 
package, excellent benefits and relocation assistance when necessary. Qualified 
candidates must be HT or HTL (ASCP) certified with prior experience working in 
a clinical lab setting.  Please let me know if you're be interested in hearing 
more about this position and learning if it might be the next step in your 
career. 

 

Below is a list of some of the opportunities we're currently working on. If you 
do not see an opening in a location in which you live or would like to live, 
please send me an email me a copy of your resume and let me know where you 
would be interested in a job. I will then tailor a search for you that is 
completely confidential.



 

IN - Columbus - Histotechnologist (1st Shift) *New Opening*


GA - Histology Supervisor


NY- Upstate- Histotech (1st Shift)


NY- New York City - Histology Supervisor 


NY - New York City - Histotech (3rd shift)


NY - New York City - Grossing Technologist *New Opening*




NV -Las Vegas - Histotech (3rd shift)


NV Las Vegas - Histology Supervisor (3rd shift)


CA - Southern - Histotech


GA - Atlanta - Histotech


MA - Histotech




MA - IHC Tech





 






















If you're interested in learning more about these opportunities or 
opportunities in a certain geographic location please reply with an updated 
resume and let me know when a good time to reach you is. If this is not the 
right time for you to make a career change then please let me know if you can 
refer someone who is.  We offer a generous referral bonus for anyone you refer 
to us that we place into any position across the country.  

 


To learn more about us please visit our website at www.ka-recruiting.com.  










 

Sincerely












KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



k...@ka-recruiting.com



www.ka-recruiting.com
  








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[Histonet] RE: Refrigerator Mold

[Weems, Joyce]

Scrape off the mold and make us all some control blocks!! 

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson 
[jander...@halozyme.com]
Sent: Tuesday, June 08, 2010 3:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks 
of folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that 
seems to have worked, but I'm not sure if that is detrimental to slide and 
reagent storage.
I have purchased some strong disinfectants also, but I don't know if those 
vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
jander...@halozyme.com




The information transmitted in this email is confidential and is intended only 
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retransmission, dissemination or other use of, or taking of any action in 
reliance upon, this information by individuals or entities other than the 
intended recipient is prohibited by Halozyme and may be in violation of 
applicable laws. If you received this in error, please contact the sender and 
delete/destroy this email.
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RE: [Histonet] Refrigerator Mold

[Jennifer MacDonald]

Bleach can cause corrosion of the metal parts.




"Sherwood, Margaret "  
Sent by: histonet-boun...@lists.utsouthwestern.edu
06/08/2010 01:36 PM

To
"Jennifer Anderson" , 

cc

Subject
RE: [Histonet] Refrigerator Mold






By folders, I assume you mean the cardboard ones.  Why not store the 
slides in
something else, i.e. plastic boxes?

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Tuesday, June 08, 2010 3:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have 
stacks of
folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and 
that
seems to have worked, but I'm not sure if that is detrimental to slide and
reagent storage.
I have purchased some strong disinfectants also, but I don't know if those
vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
jander...@halozyme.com




The information transmitted in this email is confidential and is intended 
only
for the person(s) or entity to which it is addressed. Delivery of this 
message
to any person other than the intended recipient(s) is not intended in any 
way to
waive confidentiality or any applicable privilege. Any review, 
retransmission,
dissemination or other use of, or taking of any action in reliance upon, 
this
information by individuals or entities other than the intended recipient 
is
prohibited by Halozyme and may be in violation of applicable laws. If you
received this in error, please contact the sender and delete/destroy this 
email.
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is
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e-mail
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[Histonet] Special Stain Procedures

[thisisann]

Does anyone have the following procedures they can share with me:
- Rhodamine (for copper)
- Victoria Blue
Thank you,
Ann



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RE: [Histonet] Refrigerator Mold

[Sherwood, Margaret]

By folders, I assume you mean the cardboard ones.  Why not store the slides in
something else, i.e. plastic boxes?

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Tuesday, June 08, 2010 3:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks of
folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that
seems to have worked, but I'm not sure if that is detrimental to slide and
reagent storage.
I have purchased some strong disinfectants also, but I don't know if those
vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
jander...@halozyme.com




The information transmitted in this email is confidential and is intended only
for the person(s) or entity to which it is addressed. Delivery of this message
to any person other than the intended recipient(s) is not intended in any way to
waive confidentiality or any applicable privilege. Any review, retransmission,
dissemination or other use of, or taking of any action in reliance upon, this
information by individuals or entities other than the intended recipient is
prohibited by Halozyme and may be in violation of applicable laws. If you
received this in error, please contact the sender and delete/destroy this email.
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RE: [Histonet] water bath information

[Truscott, Tom]

Hi, The old TBS's are blue, and the new ones are white. Tom T

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Tuesday, June 08, 2010 12:02 PM
To: 'Cheryl'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] water bath information

I am also looking for the same type of waterbaths and would appreciate any
information.
Thanks
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, June 08, 2010 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] water bath information

Hi Guys-
 
We need new waterbaths and I'm looking for a specific type.  It's blue with
a square glass baking dish inset on the top.  We don't care if they're blue
but the small footprint with the glass insert is the key--
 
Vendors and refurb dealer responses welcome-
 
Thanks!
 
Cheryl Kerry, HT(ASCP)
Houston
ker...@labcorp.com
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[Histonet] Waterbath Options

[Breeden, Sara]

Okay, time for my two cents' worth.  Water baths - I don't like the
glass-dish-insert kind because water can get beneath the glass dish and
can temp fluctuations. And if the glass insert breaks, it might be
difficult to find a replacement (Pyrex?).  I like the good ol' fashioned
round or rectangular black-lined fill-it-with-water-and-turn-it-on kind.
They hold temp much better and because they are lined with something
like black Teflon (or whatever), they clean like a dream.  I found that
I really do not like the romantic under-tissue lighting.  It's all about
preferences and this is mine...not that you asked,  but that never
stopped me before, did it?  Happy Tuesday!

 

Sally Breeden, HT(ASCP)

Veterinary Diagnostic Services

New Mexico Department of Agriculture

700 Camino de Salud NE

Albuquerque, NM  87108

505-841-2576

 

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[Histonet] Refrigerator Mold

[Jennifer Anderson]

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks 
of folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that 
seems to have worked, but I'm not sure if that is detrimental to slide and 
reagent storage.
I have purchased some strong disinfectants also, but I don't know if those 
vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
jander...@halozyme.com




The information transmitted in this email is confidential and is intended only 
for the person(s) or entity to which it is addressed. Delivery of this message 
to any person other than the intended recipient(s) is not intended in any way 
to waive confidentiality or any applicable privilege. Any review, 
retransmission, dissemination or other use of, or taking of any action in 
reliance upon, this information by individuals or entities other than the 
intended recipient is prohibited by Halozyme and may be in violation of 
applicable laws. If you received this in error, please contact the sender and 
delete/destroy this email.
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RE: [Histonet] water bath information

[Cynthia Pyse]

I am also looking for the same type of waterbaths and would appreciate any
information.
Thanks
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, June 08, 2010 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] water bath information

Hi Guys-
 
We need new waterbaths and I'm looking for a specific type.  It's blue with
a square glass baking dish inset on the top.  We don't care if they're blue
but the small footprint with the glass insert is the key--
 
Vendors and refurb dealer responses welcome-
 
Thanks!
 
Cheryl Kerry, HT(ASCP)
Houston
ker...@labcorp.com
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[Histonet] Away for instrument training.

[Ken_Marissael]

I will be out of the office starting  06/08/2010 and will not return until
06/14/2010.

I will be out of the field beginning June 8th and will return on June 14th.
Please call Customer Service at 1-800-932-5000 for any of your immediate
needs. I will have very limited access to e-mail and v-mail, but will try
to respond as quickly as I can.


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Re: [Histonet] block disposal

[DKBoyd]

 We "red bag" (biohazard) ours and they are incinerated.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net





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[Histonet] water bath information

[Cheryl]

Hi Guys-
 
We need new waterbaths and I'm looking for a specific type.  It's blue with a 
square glass baking dish inset on the top.  We don't care if they're blue but 
the small footprint with the glass insert is the key--
 
Vendors and refurb dealer responses welcome-
 
Thanks!
 
Cheryl Kerry, HT(ASCP)
Houston
ker...@labcorp.com
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RE: [Histonet] block disposal

[jstaruk]

We are also strictly research and avoid this situation by returning all
blocks back to the clients.

___
James E. Staruk HT(ASCP)
 www.masshistology.com
   www.nehorselabs.com
 
 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret 
Sent: Tuesday, June 08, 2010 11:36 AM
To: Joyce Cline; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] block disposal


We are a research laboratory.  Our policy, based on communication via the
Histonet, is to keep 2 years worth of paraffin blocks in the lab.  Blocks
greater than 2 years old up to 10 years old, we send to off-site storage.
Blocks greater than 10 years old are destroyed by the storage company since
they have them.

When we first set up this system last year, we had 20 years worth of blocks
and the majority were put in our red hazardous waste bucket to be
incinerated. 

Peggy   


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline
Sent: Tuesday, June 08, 2010 10:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block disposal

How does everyone handle disposing of blocks that are more than 10 years
old? Are they incinerated, hauled away by a waste management company or just
thrown in the trash.

In Maryland I was told they can't be thrown in the regular trash by a state
waste official.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941 joyce.cl...@wchsys.org



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Re: [Histonet] block disposal

[BSullivan]

We double bag our blocks in Bio Hazard bags and then they are incinerated.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


   
 Joyce Cline   
 To 
 Sent by:  "histonet@lists.utsouthwestern.edu" 
 histonet-bounces@  
 lists.utsouthwest  cc 
 ern.edu   
   Subject 
   [Histonet] block disposal   
 06/08/2010 10:27  
 AM
   
   
   
   




How does everyone handle disposing of blocks that are more than 10 years
old? Are they incinerated, hauled away by a waste management company or
just thrown in the trash.

In Maryland I was told they can't be thrown in the regular trash by a state
waste official.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cl...@wchsys.org



* CONFIDENTIALITY NOTICE * This message contains confidential
information and is intended only for the individual named. If you are not
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RE: [Histonet] block disposal

[Baez, Janet]

We donate our blocks to research. Find out if any institution in your
area wants them if not we have to incinerate them by California law.

Janet Baez
Interscope Pathology Medical Group

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: Tuesday, June 08, 2010 7:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block disposal

How does everyone handle disposing of blocks that are more than 10 years
old? Are they incinerated, hauled away by a waste management company or
just thrown in the trash.

In Maryland I was told they can't be thrown in the regular trash by a
state waste official.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cl...@wchsys.org



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Re: [Histonet] Gordon & Sweet Reticulin Stain problem on BMT Sections

[Rene J Buesa]

Usually an excess of ammonium in the amoniacal-silver solution is the culprit. 
Check this solution.
René J.

--- On Tue, 6/8/10, Malika Benatti  wrote:


From: Malika Benatti 
Subject: [Histonet] Gordon & Sweet Reticulin Stain problem on BMT Sections
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, June 8, 2010, 10:33 AM


** Proprietary **
** Reply Requested When Convenient **

Dear all,

Lately we have been experiencing problem with our Retic Stains, who keep 
lifting off slides after the gold chloride step.

We have changed all solutions one by one, trying to see which one was causing 
problem without success.
BMT Bx are cut at 3 micron on X-tra adhesive slides.
BMT BX are decal in 10 % Formic Formaldehyde.

Here is the protocol that we are currently using.

1.   Sections to water.
2.   Treat with 0.25% acidified Potassium permanganate.   --- 5 mins
3.   Bleach in oxalic acid.
4.   Rinse in distilled water.
5.   Treat with 5% iron alum.                            --- 15 mins       
6.   Rinse briefly in distilled water.
7.   Impregnate with filtered ammoniacal silver solution          ---1 min
NB: Time in ammoniacal silver may need to be increased up to 5 min for bone 
marrow trephines
8.   Rinse well in distilled water.
9.   Reduce in 10% formalin (in tap water).                    --- 1min
10.  Rinse in distilled water.
11.  Tone in 0.2% Gold Chloride                         ---1 min
12.  Rinse in distilled water.
13.  Fix in 5%  sodium thiosulphate (hypo)                    --- 30 sec
14.  Wash in water.
15.  Counterstain with neutral red.
16.  Dehydrate, clear and mount. 


Any suggestions would be  appreciated.

Malika


Malika Benatti

Specialist BMS 
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH


Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875

ben...@gosh.nhs.uk


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RE: [Histonet] block disposal

[Sherwood, Margaret]

We are a research laboratory.  Our policy, based on communication via the
Histonet, is to keep 2 years worth of paraffin blocks in the lab.  Blocks
greater than 2 years old up to 10 years old, we send to off-site storage.
Blocks greater than 10 years old are destroyed by the storage company since they
have them.

When we first set up this system last year, we had 20 years worth of blocks and
the majority were put in our red hazardous waste bucket to be incinerated. 

Peggy   


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline
Sent: Tuesday, June 08, 2010 10:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block disposal

How does everyone handle disposing of blocks that are more than 10 years old?
Are they incinerated, hauled away by a waste management company or just thrown
in the trash.

In Maryland I was told they can't be thrown in the regular trash by a state
waste official.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cl...@wchsys.org



* CONFIDENTIALITY NOTICE * This message contains confidential
information and is intended only for the individual named. If you are not the
named addressee you should not disseminate, distribute or copy this e-mail.
Please notify the sender immediately by e-mail if you have received this e-mail
by mistake and delete this e-mail from your system.
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contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
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[Histonet] Histodeck Flash Cards

[laurie]

Hi Everyone, 

I was wondering if anyone out there has used the Histodeck flash cards to study 
for the HTL exam?  Just curious.

Thanks, 
Laurie



***

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[Histonet] Gordon & Sweet Reticulin Stain problem on BMT Sections

[Malika Benatti]

** Proprietary **
** Reply Requested When Convenient **

Dear all,

Lately we have been experiencing problem with our Retic Stains, who keep 
lifting off slides after the gold chloride step.

We have changed all solutions one by one, trying to see which one was causing 
problem without success.
BMT Bx are cut at 3 micron on X-tra adhesive slides.
BMT BX are decal in 10 % Formic Formaldehyde.

Here is the protocol that we are currently using.

1.   Sections to water.
2.   Treat with 0.25% acidified Potassium permanganate. --- 5 mins
3.   Bleach in oxalic acid.
4.   Rinse in distilled water.
5.   Treat with 5% iron alum.   --- 15 mins 
   
6.   Rinse briefly in distilled water.
7.   Impregnate with filtered ammoniacal silver solution  ---1 min
NB: Time in ammoniacal silver may need to be increased up to 5 min for bone 
marrow trephines
8.   Rinse well in distilled water.
9.   Reduce in 10% formalin (in tap water). --- 1min
10.  Rinse in distilled water.
11.  Tone in 0.2% Gold Chloride  ---1 min
12.  Rinse in distilled water.
13.  Fix in 5%  sodium thiosulphate (hypo)  --- 30 sec
14.  Wash in water.
15.  Counterstain with neutral red.
16.  Dehydrate, clear and mount. 


Any suggestions would be  appreciated.

Malika


Malika Benatti
 
Specialist BMS 
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH

 
Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875
 
ben...@gosh.nhs.uk


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[Histonet] block disposal

[Joyce Cline]

How does everyone handle disposing of blocks that are more than 10 years old? 
Are they incinerated, hauled away by a waste management company or just thrown 
in the trash.

In Maryland I was told they can't be thrown in the regular trash by a state 
waste official.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cl...@wchsys.org



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by mistake and delete this e-mail from your system.
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[Histonet] What's up at the Region II Symposium, June 10,11,12?

[Barone, Carol]

Histonetters:Here are the sessions  open for your interest - Region II
Symposium: 

*   Thursday June 10: Region II Registration opens for registration
at 12:30: Session 1: Trouble shooting Histology Stains, Session 2
Manager's Forum, Friday June 11, AM -Session 1: VIR Seminars, Session 2:
Sectioning Artifacts, Session 3: Going LEAN: LEAN to Green, Session 4:
Validation/Troubleshooting IHC 
*   Venors open at noon, Lunch
*   PM - Session 1: Clinical Seminars.,Session 2: Cardio System;
Form and Function, Session 3. Principles of LEAN live simulation,
Session 4: Ergononmics f
*   Saturday 12, AM, Session 1: Safety, Session 2: Prostate Cancer
Markers, Session 3: Ultramicrotomy, Session 4: CAP Accreditation
*   Saturday PM, Session 1: Open Seminars, Session 2: Preparing for
the HT/HTL, Seeion 3: Cytoprep Techniques, Session 4: Polymer Revolution
in IHC

Friday and Satuday seessions begin at 8:00 AM ..over 30 vendors, Guest
Speaker: Peggy Wenk, and Friday evening net-working - FIESTA
Walk-ins will be accepted with no late fees! So Come on down to the
Shore! More info available @ www.nsh.org/content/region-ii-meeting
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[Histonet] VIP6

[Sheila Haas]

Hi all. Anyone out there with a VIP 6 using the cassette count as a way of 
tracking to determine reagent changes? Just curious as to what number of 
cassettes are recommended before changes. We're used to changing by number of 
runs vs number of cassettes. Thanks in advance!
 
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories



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