[Histonet] Re: Histonet Digest, Vol 79, Issue 22
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[Histonet] Re: Histonet Digest, Vol 79, Issue 22
___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 79, Issue 22
___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] beta-myosin heavy chain (Myh7) antibody
Does anyone know of a supplier for an antibody against beta-myosin heavy chain (Myh7) that works in mouse tissue and have experience working with it? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
One thing I noticed was: "*Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* and then everything else became clear. Mary Abosso Denver, CO From: histonet-boun...@lists.utsouthwestern.edu on behalf of Mike Pence Sent: Fri 6/18/2010 2:12 PM To: Thomas Jasper; Mark Tarango Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Tom I did notice that too and wondered just how long this person had been "out" of the working lab setting. This was something that was done back when IHC was done all manually. I think I will just take my chances with what I am doing now as acceptable and wait and see what the CAP inspector thinks or at least how they are dealing with this question! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Friday, June 18, 2010 2:38 PM To: Mark Tarango Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 11:47 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpas...@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran > from the previous lot and compare it to the slide that you have >
Re: [Histonet] New CAP question ANP.22760
http://www.thefreedictionary.com/contemporaneously From: "anni...@gmail.com" To: Mark Tarango ; Histonet Sent: Fri, June 18, 2010 3:38:01 PM Subject: Re: [Histonet] New CAP question ANP.22760 Am following this IHC/CAP thread with great interest, but I have to ask...what is the origin of the word 'contemporaneously'? English is my mother tongue but this word is new to me- simultaneous and contemporary make sense but this 'amalgamation' is an abomination! Annie. Anyone? Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Mark Tarango Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 18 Jun 2010 11:46:53 To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the “old” lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpas...@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran from > the previous lot and compare it to the slide that you have stained with the > new lot number. To see if they are sufficient diagnostic quality. Not put > both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpe...@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee >_ > > From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] > To: Ellen Yee [mailto:e...@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utso
RE: [Histonet] New CAP question ANP.22760
They're taking lessons from our former president of the United St
ates!! =)
Sarah Goebel, B.A., HT (ASCP)
<= /em>
Histotechnicia= n
XBiotech USA Inc.
<= div>8201 East Riverside Dr. B= ldg 4 Suite 100
Austin, Texas 78744
<= div>
(512)= 386-5107
Original Message
Subject: Re: [Histonet] New CAP question ANP.22760
From: [1]anni...@gmail.com
Date: Fri, June 18, 2010 1:38 pm
To: "Mark Tarango" <[2]marktaran= g...@gmail.com>, "Histonet"
<[3]histo...@lists.u= tsouthwestern.edu>
Am following this IHC/CAP thread with great interest, but I have to
ask...w= hat is the origin of the word 'contemporaneously'?
English is my mother tongue but this word is new to me- simultaneous
and co= ntemporary make sense but this 'amalgamation' is an
abomination!
Annie.
Anyone?
Empower your Business with BlackBerry® and Mobile Solutions from
Etisa= lat
-Original Message-
From: Mark Tarango <[4]marktaran= g...@gmail.com>
Sender: [5]hist= onet-boun...@lists.utsouthwestern.edu
Date: Fri, 18 Jun 2010 11:46:53
To: McMahon, Loralee A<[6]loralee_mcma...@urmc.rochester.edu>
Cc: [7]histo...@lists.u
tsouthwestern.edu<[8]histo...@lists.utsouthwestern.edu>
Subject: Re: [Histonet] New CAP question ANP.22760
That's what I thought at first too. It might be helpful to post this
lette= r
that I got from the CAP about this. I tried to argue with them, but
this i= s
the answer I got.
Dear Mark,
Your questions were forwarded to me for response.
During the Audio-conference, the idea of comparing a previously
stained
slide (that had used the “old” lot) to one stained
with the= new lot was
deemed acceptable, but not optimal. Doing a simultaneous staining
using old and new lots, better demonstrates the performance
characteristics of
the
reagent. The reason parallel staining is considered best practice is
that<= br> all other variables, such as variations in the lot of
detection reagent or<= br> instrument function, are eliminated from
consideration when the slides are<= br> stained contemporaneously.
The antibody "getting weak over time" should not happen to a
significant
degree if the antibody is used within its expiration date. If the lab
is having this kind of trouble, it should look carefully at its
storage
conditions.
Demonstrating acceptable performance of the new lot, before being
place int= o
service, is *required* for all accredited laboratories.
To answer the last question, the key is to order the new reagent well
befor= e
you run out of the old lot so that the parallel stain can be performed
before the old lot is consumed. One multi-tissue slide control slide
would<= br> suffice to evaluate a primary antibody lot in most cases,
which helps to
minimize the impact on the lab.
I hope that this information is helpful. Thank you for your
participation<= br> in the Laboratory Accreditation Program.
Sincerely,
*Kathy Passarelli, MT(ASCP)SBB*
*Technical Specialist*
*Laboratory Accreditation Program*
*College** of American** Pathologists*
*Phone: 1-(800)-323-4040 ext 7486*
*e-mail: [9]**kpas...@cap.org* <= ;[10]kpas...@cap.org>
On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A <
[11]loralee_mcma...@urm= c.rochester.edu> wrote:
> I think that CAP means that you need to save the slide that you ran
fr= om
> the previous lot and compare it to the slide that you have stained
wit= h the
> new lot number. To see if they are sufficient diagnostic quality.
No= t put
> both lot numbers on the machine at the same time and then compare
the<= br> > slides? We run Dako machines and it would be tricky to put
both numb= ers on
> the same machine.
>
> Although this is my interpretation.
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
>
> From: [12]h= istonet-boun...@lists.utsouthwestern.edu [
> [13]histone= t-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence [
> [14]mpe...@grhs.net]
> Sent: Friday, June 18, 2010 12:41 PM
> To: Ellen Yee; Laurie Colbert
> Cc: [15]histo...@l= ists.utsouthwestern.edu
> Subject: RE: [Histonet] New CAP question ANP.22760
>
> I don't think I can do this with the automated system we are
currently > using. Ventana. Does any other Ventana users know if you can
do this
i= n
> "parallel"
>
> Mike
> -Original Message-
> From: [16]h= istonet-boun...@lists.utsouthwestern.edu
> [[17]mailto:h= istonet-boun...@lists.utsouthwestern.edu] On Behalf
Of Ellen
> Yee
> Sent: Thursday, June 17, 2010 7:21 PM
Re: [Histonet] New CAP question ANP.22760
Hi Tom, I did notice. In my original question, I had said something like "is it possible that the Pathologists that gave the teleconference are somewhat out of touch with the realities of doing this in the lab?" Instead of the speakers writing back, I got her response. I was just glad that she did say it was acceptable (but not best practice) to compare against a previosly stained slide. In the teleconference he was very clear that he wanted the slides to be stained side-by-side. Mark On Fri, Jun 18, 2010 at 12:38 PM, Thomas Jasper wrote: > Mark, > > Did you notice the credentials from this CAP representative? MT with a > Blood Bank specialty I believe. What I glean from that is...more than > likely this person does not grasp the logistics of "contemporaneously" > staining identical Abs from separate lots. She also likely does not > understand the logistical application for detection and automation > either. > > I'm not trying to be overly critical of this person. I'm sure she is > quite intelligent and would not have the MT/SBB if she wasn't > intelligent. It comes down to a lack of understanding Anatomic > Pathology testing application re: automated IHC. I believe this is a > common problem in and out of CAP. Many lab directors and other folks in > positions of authority without AP/Histology/Cytology backgrounds seem to > believe that broad clinical lab modalities apply to Anatomic Path > scenarios. I used to refer to this in my former position as - "Trying > to put the yoke of clinical lab onto anatomic path." We are > laboratorians, but in many instances do not fit the general clinical lab > mold. > > It's unfortunate that CAP has put this person in the position to > respond. It is apparent to me that she's not grasping the particulars > here. She probably never will unless she decides to go into a working, > automated IHC "tissue" lab and take the time to ask questions and > understand (learn) what we're all about. > > Thanks, > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, OR 97701 > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark > Tarango > Sent: Friday, June 18, 2010 11:47 AM > To: McMahon, Loralee A > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] New CAP question ANP.22760 > > That's what I thought at first too. It might be helpful to post this > letter that I got from the CAP about this. I tried to argue with them, > but this is the answer I got. > > > Dear Mark, > > Your questions were forwarded to me for response. > > > > During the Audio-conference, the idea of comparing a previously stained > slide (that had used the "old" lot) to one stained with the new lot was > deemed acceptable, but not optimal. Doing a simultaneous staining using > old and new lots, better demonstrates the performance characteristics of > the reagent. The reason parallel staining is considered best practice > is that all other variables, such as variations in the lot of detection > reagent or instrument function, are eliminated from consideration when > the slides are stained contemporaneously. > > > > The antibody "getting weak over time" should not happen to a significant > degree if the antibody is used within its expiration date. If the lab > is having this kind of trouble, it should look carefully at its storage > conditions. > > > > Demonstrating acceptable performance of the new lot, before being place > into service, is *required* for all accredited laboratories. > > > > To answer the last question, the key is to order the new reagent well > before you run out of the old lot so that the parallel stain can be > performed before the old lot is consumed. One multi-tissue slide control > slide would suffice to evaluate a primary antibody lot in most cases, > which helps to minimize the impact on the lab. > > > > I hope that this information is helpful. Thank you for your > participation in the Laboratory Accreditation Program. > > > > Sincerely, > > > > *Kathy Passarelli, MT(ASCP)SBB* > > *Technical Specialist* > > *Laboratory Accreditation Program* > > *College** of American** Pathologists* > > *Phone: 1-(800)-323-4040 ext 7486* > > *e-mail: **kpas...@cap.org* > > > > On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < > loralee_mcma...@urmc.rochester.edu> wrote: > > > I think that CAP means that you need to save the slide that you ran > > from the previous lot and compare it to the slide that you have > > stained with the new lot number. To see if they are sufficient > > diagnostic quality. Not put both lot numbers on the machine at the > same time and then compare the > > slides? We run Dako machines and it would be tricky to put both > numbers on > > the same machine. > > > > Although this is my interpretation. > > > > Loralee McMahon, HTL (ASCP) > > Immunohistochemistry Supervisor > > St
Re: [Histonet] New CAP question ANP.22760
Am following this IHC/CAP thread with great interest, but I have to ask...what is the origin of the word 'contemporaneously'? English is my mother tongue but this word is new to me- simultaneous and contemporary make sense but this 'amalgamation' is an abomination! Annie. Anyone? Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Mark Tarango Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 18 Jun 2010 11:46:53 To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the “old” lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpas...@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran from > the previous lot and compare it to the slide that you have stained with the > new lot number. To see if they are sufficient diagnostic quality. Not put > both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpe...@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee >_ > > From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] > To: Ellen Yee [mailto:e...@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > >
RE: [Histonet] PPARalpha
We tried the ppar from abcam and was not to impressed by it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Peter Boor Sent: Friday, June 18, 2010 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PPARalpha Hi everyone, Does anyone has experience with PPARalpha staining in rats and mice (in kidneys)? Which antibodies work? I have tried the Abcam polyclonal rabbit (ab8934) Ab that is supposed to work, but it is not (tried methyl carnoyls solution, formalin, frozen sections and various Ag retrievals). I would be very grateful for any suggestions! Many thanks in advance, Peter P E T E RB O O R, MD, PhD Dpt. of Nephrology & Inst. of Pathology, RWTH University Aachen Pauwelstr. 30 52074 Aachen, Germany Tel.:+49 241 80 89670 E-Mail: b...@email.cz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Breast Specimen handling
Kathy: After 48-72 hrs 10% NBF we wash our cassettes during 10-15 mins in tap water, then store it in 70% isopropyl alcohol (IPA) before processing. You can store tissues here as long as need. The IPA does not harden it as ethanol. We using as dehydrant also IPA. Sincerely, Maxim Peshkov, Taganrog, Russia. ---Original message--- > Date: Thu, 17 Jun 2010 13:24:22 -0400 > From: "Sara Baldwin/mhhcc.org" > Subject: [Histonet] Breast Specimen handling > To: Histo Net > Message-ID: > > > > Content-Type: text/plain; charset="ISO-8859-1" > > >Hi histonetters > >Was wondering if anyone saw the new gui > specimen handling ? I am sure you have.&nbs is > wondering is if you have a large specimen and do > tissue for processing what would the agent be > to apply specimen to stop penetration of the > formlalin before the 72 hour ti me arrives? Would > it be sodium chloride? Can someone help? >< Thanks >Pathology Supervisor >Kathy Baldwin, S Memorial Hospital and Health > Care&nbs [1]sbald...@m Ph 812-482-0210, 482-0216, > Fax 81 Pager 812-481-0897 >Confidential information, Authorized use only. > > References > >1. 3D"mailto:sbald...@mhhcc.org"; mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
Tom I did notice that too and wondered just how long this person had been "out" of the working lab setting. This was something that was done back when IHC was done all manually. I think I will just take my chances with what I am doing now as acceptable and wait and see what the CAP inspector thinks or at least how they are dealing with this question! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Friday, June 18, 2010 2:38 PM To: Mark Tarango Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 11:47 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpas...@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran > from the previous lot and compare it to the slide that you have > stained with the new lot number. To see if they are sufficient > diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > _
[Histonet] PPARalpha
Hi everyone, Does anyone has experience with PPARalpha staining in rats and mice (in kidneys)? Which antibodies work? I have tried the Abcam polyclonal rabbit (ab8934) Ab that is supposed to work, but it is not (tried methyl carnoyls solution, formalin, frozen sections and various Ag retrievals). I would be very grateful for any suggestions! Many thanks in advance, Peter P E T E RB O O R, MD, PhD Dpt. of Nephrology & Inst. of Pathology, RWTH University Aachen Pauwelstr. 30 52074 Aachen, Germany Tel.:+49 241 80 89670 E-Mail: b...@email.cz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: test
re > test > > _ > > From: Thomas Jasper > Sent: Friday, June 18, 2010 11:17 AM > To: 'histonet-boun...@lists.utsouthwestern.edu' > Subject: test > > > Thanks, checking on connection. > tj > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 11:47 AM To: McMahon, Loralee A Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the "old" lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpas...@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran > from the previous lot and compare it to the slide that you have > stained with the new lot number. To see if they are sufficient > diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpe...@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this > in "parallel" > > Mike > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent
RE: [Histonet] New CAP question ANP.22760
As I have found over the years the opinions and interpretation of CAP intent is differing. Everyone has how they see the question and think it to best be answered. I am not sure why it is not acceptable for the lab to run a control on the new lot and make sure it works and that is the QC/QA. The pathologist is the one that determines if the stain is acceptable for diagnosis. Some of the suggestions would be very time consuming and costly to do each new lot#. -Original Message- From: Vickroy, Jim [mailto:vickroy@mhsil.com] Sent: Friday, June 18, 2010 1:06 PM To: Mark Tarango; Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 I'm kind of getting in on the middle of this but is anybody else doing this with prep kit stickers? I have not seen the new question but has anyone talked to CAP to get a clarification of what they mean on this question? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 12:27 PM To: Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 You'll have to use prep kit stickers and duplicate protocols to do it, but it is possible. You just need to copy the protocol and save it as another number, then change the primary antibody to a prep kit sticker save again and then put that sticker on the dispenser. Then you need to print stickers for both protocols and stick them on two controls slides and run. I admit its a little bit of a pain. Mark On Fri, Jun 18, 2010 at 9:41 AM, Mike Pence wrote: > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this > in "parallel" > > Mike > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents > tested in parallel with old lots? (NOTE: New lots of primary antibody > and detection system reagents must be compared to the previous lot > using an appropriate panel of control tissues.) > > Ellen Yee > _ > > From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] > To: Ellen Yee [mailto:e...@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] New CAP question ANP.22760
That's what I thought at first too. It might be helpful to post this letter that I got from the CAP about this. I tried to argue with them, but this is the answer I got. Dear Mark, Your questions were forwarded to me for response. During the Audio-conference, the idea of comparing a previously stained slide (that had used the “old” lot) to one stained with the new lot was deemed acceptable, but not optimal. Doing a simultaneous staining using old and new lots, better demonstrates the performance characteristics of the reagent. The reason parallel staining is considered best practice is that all other variables, such as variations in the lot of detection reagent or instrument function, are eliminated from consideration when the slides are stained contemporaneously. The antibody "getting weak over time" should not happen to a significant degree if the antibody is used within its expiration date. If the lab is having this kind of trouble, it should look carefully at its storage conditions. Demonstrating acceptable performance of the new lot, before being place into service, is *required* for all accredited laboratories. To answer the last question, the key is to order the new reagent well before you run out of the old lot so that the parallel stain can be performed before the old lot is consumed. One multi-tissue slide control slide would suffice to evaluate a primary antibody lot in most cases, which helps to minimize the impact on the lab. I hope that this information is helpful. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, *Kathy Passarelli, MT(ASCP)SBB* *Technical Specialist* *Laboratory Accreditation Program* *College** of American** Pathologists* *Phone: 1-(800)-323-4040 ext 7486* *e-mail: **kpas...@cap.org* On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A < loralee_mcma...@urmc.rochester.edu> wrote: > I think that CAP means that you need to save the slide that you ran from > the previous lot and compare it to the slide that you have stained with the > new lot number. To see if they are sufficient diagnostic quality. Not put > both lot numbers on the machine at the same time and then compare the > slides? We run Dako machines and it would be tricky to put both numbers on > the same machine. > > Although this is my interpretation. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [ > mpe...@grhs.net] > Sent: Friday, June 18, 2010 12:41 PM > To: Ellen Yee; Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee > _ > > From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] > To: Ellen Yee [mailto:e...@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histone
[Histonet] RE: IHC kits
You could try Biocare regents. I use many Biocare reagents. Or sign a contract with Dako to lock in your prices... Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mauger, Joanne [mau...@email.chop.edu] Sent: Friday, June 18, 2010 1:59 PM To: Charles, Roger; Histonet(histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: IHC kits The Leica Bond detection kits are better than LSAB. They are barcoded for the Bondmax machine-not sure if they offer packaged another way. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger [rchar...@state.pa.us] Sent: Friday, June 18, 2010 10:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC kits Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: test
test _ From: Thomas Jasper Sent: Friday, June 18, 2010 11:17 AM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: test Thanks, checking on connection. tj ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
Regarding this question. This is my take on this. You always have to check Lot to Lot antibodies and make sure you have consistent results. We have a ledger where our new lots are recorded when received into the lab. There is also a place for all the necessary information about the antibody, the lot number , open date, Q.C. date and the antibody's expiration date. Once the antibody is opened it is Q.C'd on tissue that we have used in the past for control material. The control is checked and it is noted if the results are favorable or not. If there are favorable results it is put into production. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 "McMahon, Loralee A" , Ellen u>Yee , Laurie Sent by: Colbert histonet-bounces@ ern.educc "histonet@lists.utsouthwestern.edu" 06/18/2010 01:47 Subject PMRE: [Histonet] New CAP question ANP.22760 I think that CAP means that you need to save the slide that you ran from the previous lot and compare it to the slide that you have stained with the new lot number. To see if they are sufficient diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the slides? We run Dako machines and it would be tricky to put both numbers on the same machine. Although this is my interpretation. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpe...@grhs.net] Sent: Friday, June 18, 2010 12:41 PM To: Ellen Yee; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _ From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] To: Ellen Yee [mailto:e...@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@list
RE: [Histonet] New CAP question ANP.22760
I'm kind of getting in on the middle of this but is anybody else doing this with prep kit stickers? I have not seen the new question but has anyone talked to CAP to get a clarification of what they mean on this question? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, June 18, 2010 12:27 PM To: Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New CAP question ANP.22760 You'll have to use prep kit stickers and duplicate protocols to do it, but it is possible. You just need to copy the protocol and save it as another number, then change the primary antibody to a prep kit sticker save again and then put that sticker on the dispenser. Then you need to print stickers for both protocols and stick them on two controls slides and run. I admit its a little bit of a pain. Mark On Fri, Jun 18, 2010 at 9:41 AM, Mike Pence wrote: > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee > _ > > From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] > To: Ellen Yee [mailto:e...@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC kits
The Leica Bond detection kits are better than LSAB. They are barcoded for the Bondmax machine-not sure if they offer packaged another way. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger [rchar...@state.pa.us] Sent: Friday, June 18, 2010 10:38 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC kits Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
I think that CAP means that you need to save the slide that you ran from the previous lot and compare it to the slide that you have stained with the new lot number. To see if they are sufficient diagnostic quality. Not put both lot numbers on the machine at the same time and then compare the slides? We run Dako machines and it would be tricky to put both numbers on the same machine. Although this is my interpretation. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpe...@grhs.net] Sent: Friday, June 18, 2010 12:41 PM To: Ellen Yee; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _ From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] To: Ellen Yee [mailto:e...@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 79, Issue 21
Any pros/cons with leica cm1950 against thermofisher hm550/fumigation option with cryostats? Two to four days a week using cryostat. Durability issues Thanks and enjoy the weekend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] New CAP question ANP.22760
You'll have to use prep kit stickers and duplicate protocols to do it, but it is possible. You just need to copy the protocol and save it as another number, then change the primary antibody to a prep kit sticker save again and then put that sticker on the dispenser. Then you need to print stickers for both protocols and stick them on two controls slides and run. I admit its a little bit of a pain. Mark On Fri, Jun 18, 2010 at 9:41 AM, Mike Pence wrote: > > I don't think I can do this with the automated system we are currently > using. Ventana. Does any other Ventana users know if you can do this in > "parallel" > > Mike > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Thursday, June 17, 2010 7:21 PM > To: Laurie Colbert > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] New CAP question ANP.22760 > > > Sorry, I should have included it. > > ANP.22760 Are new lots of antibody and detection system reagents tested > in parallel with old lots? (NOTE: New lots of primary antibody and > detection system reagents must be compared to the previous lot using an > appropriate panel of control tissues.) > > Ellen Yee > _ > > From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] > To: Ellen Yee [mailto:e...@dpmginc.com] > Sent: Thu, 17 Jun 2010 08:47:38 -0700 > Subject: RE: [Histonet] New CAP question ANP.22760 > > Can you give us the wording of that question/checklist item? Laurie > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen > Yee > Sent: Wednesday, June 16, 2010 10:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New CAP question ANP.22760 > > How are IHC labs complying with this question? What is considered an > appropriate panel of control tissues? What do you stain to test your > detection systems? > > Ellen Yee > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
I do this but it has to be separate runs or wait until the kit is just running out. I also keep the slides from the initial QC run and compare it with the new lot to assure same intensity staining is obtained when changing lots. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com >>> "Mike Pence" 6/18/2010 11:41 AM >>> I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _ From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] To: Ellen Yee [mailto:e...@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryojane question
I've used it for serial frozen sections in the past; worked very well for that application. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, June 18, 2010 9:10 AM To: Kim Merriam; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryojane question Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
I don't think I can do this with the automated system we are currently using. Ventana. Does any other Ventana users know if you can do this in "parallel" Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 7:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _ From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] To: Ellen Yee [mailto:e...@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] JB-4
I am still struggling when I have a spare minute to get our "inherited" JB-4 Sorvall manual microtome to work for paraffin sectioning. Now the micron knob moves freely and I can't get it to stay set on the thickness. If I get it on say 5 microns as you are cutting it drifts so you never know what thickness you are going to get. Should I just scrap this old machine or does anyone know how I need to adjust something ? As you probably can tell I am not mechanically inclined. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: cryojane question
To Kim, What thickness are you using to cut these? I'm simply amazed you're having trouble keeping them on using the 4x slides and 2 flashes. Have you tried using the 1/2x or even the 1x slides? Did they wash worse using these? Are they fixed at any time, or fresh frozen and sectioned? To Emily, I don't think the UV flash would affect the ISH at all. To Liz, We share your experience with it. The undecalcified bone and other hard tissues are very tricky to work with, and the quality of our efforts really do vary from day to day. Using this to section cartilage and decalcified bone should be pretty easy and satisfying. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] porcine intervertebral disc
Jennifer, If you can gently remove the disc, section appropriately and process for cross-section cuts you will get nice results. You might want to extend the paraffin times for very good infiltration. If the disc/bone placement needs to be in place, it becomes more difficult as the decal is hard on the disc tissue and porcine bone take a long time to decal. With disc/bone embedding and sectioning the soft and hard differences in the tissue are problematic. I suggest sectioning thicker. It can be done and the results beautiful! Vicki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC kits
We use Vectastain kits and they always work well. I don't know if you can use them for your particular IHC but here's their site www.vectorlabs.com Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Fri, Jun 18, 2010 at 10:38 AM, Charles, Roger wrote: > Happy Friday to all, > Dako has once again raised their prices to the point that I'm asked to find > another IHC labeling kit that is cheaper. Could those doing IHC please > recommend a kit that is as good as Dako's LSAB2? > Thanks so much. > Roger > > Roger Charles > Microbiologist II > PA Veterinary Laboratory > 717-787-8808 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dermatopathology
Most pathologists I have worked with (some are specialized as der
mapathologists) work with a group if it's a clinical setting. There
a= re specializations they do to become one type or another (heme
guys, derm g= uys, cyto guys, etc.) The clinical ones I have worked
with own part o= f the company that they work with (and they make a
crap ton!). The tw= o different research places I now have worked for
pay them a salary, but I = have heard of some getting a per slide
charge. Good luck, I think it'= s all up to you and what you can work
out with the docs! =)
Sarah Goebel,= B.A., HT (ASCP)
Histotechnician
XBiotech US= A Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
A= ustin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] Dermatopathology
From: Alyssa Peterson <[1]aly...@alliedsearchpartners.com>
Date: Fri, June 18, 2010 7:10 am
To: [2]histo...@lists.u= tsouthwestern.edu
Hello,
I know this might be a little out of the rhelm of histonet but, I
thought I would give it a try.
I am struggling to figure out how a Dermatopathologist is paid? Is
he/she paid per slide? Or is he/she paid a base salary, or both? I
would just
really like an idea. Thank you for any help.
--
Alyssa Peterson, Director of Candidate Recruitment
Allied Search Partners
T: 888.388.7571 ext. 101
F: 888.388.7572
[3]www.alliedsearchpartners.co= m
This email including its attachments is intended only for the
confidential<= br> use of the individual to whom it is addressed. If
you are not the intended<= br> recipient, any use, dissemination,
distribution or copying of this message<= br> or its attachments is
prohibited. If you have received this message in
error, please notify us immediately, and delete this message and its
attachments permanently from your system.
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References
1. 3D"mailto://aly...@alliedsearchpartners.c=/
2. 3D"mailto://histonet@lists.utsouthwestern.edu"/
3. 3D"http://www.alliedsearchpartners.com"/
4. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
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RE: [Histonet] ISH on histogel cell pellets
Try using 0.9% plain 'ol agar. I use it all the time, it's = cheaper
then the histogel and it works fine with the IHCs. I don't kn= ow
about ISH, but it's a thought (and a super cheap trial?)
<= br>
Sarah Goebel, B= .A., HT (ASCP)
Histotechnician
<= /em>
XBiotech USA I= nc.
8201 East Riverside Dr. Bldg 4 Suite 100
Aust= in, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] ISH on histogel cell pellets
From: "Liz Chlipala" <[1]...@premie= rlab.com>
Date: Fri, June 18, 2010 7:46 am
To: "Kim Merriam" <[2]kmerriam2= 0...@yahoo.com>, "Histonet"
<[3]histo...@lists.u= tsouthwestern.edu>
Kim the histogel should not interfere with the IHC staining, I'm not
sure a= bout the ISH
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
[4]www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-Original Message-
From: [5]histon= et-boun...@lists.utsouthwestern.edu
[[6]mailto:histonet-boun...@lists.utsouthwestern.edu= ] On Behalf Of
Kim Merriam
Sent: Friday, June 18, 2010 7:09 AM
To: Histonet
Subject: [Histonet] ISH on histogel cell pellets
Hi Everyone,
We have some cells that we have processed and embedded in histogel
(thanks&= nbsp;to everyone for the protocols that were sent to me a
couple of we= eks ago).
We are planning to do IHC and ISH on these pellets. Will the his
togel interfere with the ISH procedure at all?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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1. 3D"mailto://l...@premierlab.com"/
2. 3D"mailto://kmerriam2...@yahoo.com"/
3. 3D"mailto://histonet@lists.utsouthwestern.edu"/
4. 3D"http://www.premierlab.com"/
5. 3D"mailto://histonet-boun...@lists.utsouthwestern.edu"/
6. 3D"mailto:histonet-bounces 7.
3D"mailto://Histonet@lists.utsouthwestern.edu"/
8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
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[Histonet] PARTS FOR HACKER/MEISEI COVERSLIPPER
We have a glass coverslipper that is not currently working because we need a diaphragm for the vacuum pump in it. Our repair people tell us we have to get it from Japan and it will take eight weeks. Anybody have an idea where we can get a new or used vacuum pump for this coverslipper? We have budgeted for a new coverslipper in October but that is a long way off. Surely someone has an old coverslipper that we could get the pump off of for a decent price. Any help would be greatly appreciated. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryojane question
Does this kind of seem unnecessary? What are the applicatio= ns that
would make this Cryo-tape beneficial. I have always used the
paintbrush method which takes 3 seconds, and has always worked
perfect.&nbs= p; This looks like it would take several minutes? It
looks cool and a= ll, but just confused as to what application you
need this for; it's defini= tely not cheaper than the brush?
Sarah Goebel, B.A., HT (ASCP)<= /div>
Histotechnician
XBiotech USA Inc.
<= em>
8201 East = Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: Re: [Histonet] cryojane question
From: Emily Sours <[1]talulahgos= h...@gmail.com>
Date: Fri, June 18, 2010 7:10 am
To: Kim Merriam <[2]kmerriam200= 3...@yahoo.com>,
[3]histo...@lists.utsou= thwestern.edu
Histonetters,
I just looked up what a cryojane was, and it's pretty neat!
Does anyone else use this?
The one flaw seems to be that you can only put one section on a slide
(or a= t
least that the way it's depicted here:
[4]htt= p://www.instrumedics.com/cryojanetapetransferprocess.htm )
which makes it pretty time consuming. Also does the uv step interfere
with in situ protocols? I guess not since the DNA/RNA is already
transcribed and fixed and therefore wouldn't be mutated.
Emily
Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
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References
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2. 3D"mailto://kmerriam2...@yahoo.com"/
3. 3D"mailto://histonet@lists.utsouthwestern.edu"/
4. 3D"http://www.instrumedics.com/cryojanetapetransferprocess.htm";
5. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
6. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
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RE: [Histonet] ISH on histogel cell pellets
Kim the histogel should not interfere with the IHC staining, I'm not sure about the ISH Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, June 18, 2010 7:09 AM To: Histonet Subject: [Histonet] ISH on histogel cell pellets Hi Everyone, We have some cells that we have processed and embedded in histogel (thanks to everyone for the protocols that were sent to me a couple of weeks ago). We are planning to do IHC and ISH on these pellets. Will the histogel interfere with the ISH procedure at all? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryojane question
Emily You can put more than one section on a slide if you need to, but in our experience it does not work as nicely as depicted all of the time, it can be a bit tricky to work with on undecalcifed bone and harder tissues. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, June 18, 2010 8:10 AM To: Kim Merriam; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryojane question Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC kits
Happy Friday to all, Dako has once again raised their prices to the point that I'm asked to find another IHC labeling kit that is cheaper. Could those doing IHC please recommend a kit that is as good as Dako's LSAB2? Thanks so much. Roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dermatopathology
Hello, I know this might be a little out of the rhelm of histonet but, I thought I would give it a try. I am struggling to figure out how a Dermatopathologist is paid? Is he/she paid per slide? Or is he/she paid a base salary, or both? I would just really like an idea. Thank you for any help. -- Alyssa Peterson, Director of Candidate Recruitment Allied Search Partners T: 888.388.7571 ext. 101 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryojane question
Histonetters, I just looked up what a cryojane was, and it's pretty neat! Does anyone else use this? The one flaw seems to be that you can only put one section on a slide (or at least that the way it's depicted here: http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which makes it pretty time consuming. Also does the uv step interfere with in situ protocols? I guess not since the DNA/RNA is already transcribed and fixed and therefore wouldn't be mutated. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ISH on histogel cell pellets
Hi Everyone, We have some cells that we have processed and embedded in histogel (thanks to everyone for the protocols that were sent to me a couple of weeks ago). We are planning to do IHC and ISH on these pellets. Will the histogel interfere with the ISH procedure at all? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New CAP question ANP.22760
Should these slides be retained? If so, how long? Or is it enough just to have the documentation? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Ellen Yee Sent: Thursday, June 17, 2010 8:21 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Sorry, I should have included it. ANP.22760 Are new lots of antibody and detection system reagents tested in parallel with old lots? (NOTE: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues.) Ellen Yee _ From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] To: Ellen Yee [mailto:e...@dpmginc.com] Sent: Thu, 17 Jun 2010 08:47:38 -0700 Subject: RE: [Histonet] New CAP question ANP.22760 Can you give us the wording of that question/checklist item? Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Yee Sent: Wednesday, June 16, 2010 10:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CAP question ANP.22760 How are IHC labs complying with this question? What is considered an appropriate panel of control tissues? What do you stain to test your detection systems? Ellen Yee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet]
Dear All, I have a PFA fixed liver frozen sections and I want to stain for cd45.1, cd45.2 and f4/80. Can you guys please help me with the protocols for the staining. I would like to do Immunofluroscence. Thank you all in advance. Many Thanks, Anil Kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryojane question
Happy Friday! We recently purchased a cryojane for sectioning frozen human and rat cartilage. I am having some issues with my cartilage sections. I am using the 4X slides with 2 flashes. The sections look beautiful, until I put them into buffer to do staining. I am getting folds and the sections are lifting off during IHC staining. I am staining either by hand (on flat trays, old-fashioned style) or in the sequenza racks. Luckily, the cartilage is not not coming off the slides completely, so I have been able to get the data I need. I was wondering if anyone had any tips for me to prevent the folds or the lift-off. Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
