Re: [Histonet] bat wing histology
Hi, We did this on derm punch biopsies for a while. We used PLP (paraformaldehyde-lysine periodate) fixative. This was also done on a sliding microtome at 50um with piles of dry ice to keep the sucrose frozen. It was a bit of a pain in the butt, but we got decent results. I think the sectioning process could have been easier, but the PI was obsessed with not modifying the project at all, even if it meant improvements. We stained these as floating sections in 96 well plates, so in this case I would be concerned that the wing rolls would un-roll if you were to do the swiss roll thing I described. Have fun :-) Amos On Tue, Jul 6, 2010 at 5:10 PM, Mohit Chadha wrote: > Thank you everyone for replying, much appreciated. > > Having also talked to people in my dept, I have a rudimentary protocol > ready. Of course, I will have to tweak it to see what works. I will be using > anti PGP9.5 antibody for neuronal immunology. > > I am still not sure how to section the wing. In most likelihood, I will be > using freezing sliding microtome. The "swiss roll" method sound good and I > will definitely try it. I am thinking that since the wing membrane is thin > (~30 um), I will also try to use the whole mount of small pieces. > > Any other thoughts and advice would be appreciated. > > Thank you, > Mohit Chadha, > Univ of Maryland. > > > > > > > > On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > >> Hi, >> I do hope you are looking at cross sections of the wing and not the >> flat. That would be very difficult indeed. For good cross sections I would >> try a "Swiss Roll". This is a way of demonstrating a large amount of cross >> sectional area in small space. Take the membrane and fix it by submersion in >> the fixative of your choice. Prior to processing roll the whole membrane up >> then cut the membrane log into sections small enough to fit in a cassette. >> You can use foam biopsy pads to support this shape. Embed it and section it >> on edge to show a long coiled membrane. The hairs should be able to be >> displayed in this way as well. To show a lot of membrane at the same time >> you could place multiple rolls in one cassette. This should work well. >> >> Good Luck, >> Amos >> >> >> Message: 21 >> Date: Thu, 1 Jul 2010 11:46:17 -0400 >> From: Mohit Chadha >> Subject: [Histonet] bat wing histology >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello everyone, >> >> This is my very first post and I am desperately looking for help. I am new >> to histology, so any help would be much appreciated. >> >> I am studying the peripheral sensory innervation of bat wings. As a first >> step, I would like to demonstrate the innervation pattern on the different >> parts of the wing membrane (a whole mount of the wing?). Second, I would >> like to demonstrate the mechanoreceptor make-up of the tiny hairs on the >> wing membrane. >> >> Bat wings are highly elastic, with numerous folds, a thickness of about >> 35-45 microns (in the species I study), a network of thin collagen >> bundles, >> and pigmented superficial epidermal layers. >> I could provide more information if required. >> >> Hoping to hear back from the members. >> Thank you. >> > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Has anyone used a biotin block in their antibody diluent?
Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Rnase free slides?
Honestly, I think RNases are a bunch of hooha. If you're being careful anyway because you're doing a PCR, that should be enough. Wear gloves, be sterile. When I worked with a Russian post-doc, she said she did RNA in situ hybridization without gloves and it worked. Of course, god only knows what her protocol was, as she had some crazy stories about Russian labs. Emily -- Dark Pictures, thrones and stones that pilgrims kiss And poems that take a thousand years to die But ape the immortality of this Red label on a little butterfly. -Vladimir Nabokov, concluding stanza of ‘A Discovery’ 1941. On Tue, Jul 6, 2010 at 3:49 PM, Caroline Bass wrote: > Hello, > > I'm doing RNA work for the first time. My plan is to take a fresh rat brain, > block quickly, freeze by immersing in dry-ice cooled isopentane, storing at > -80, collecting tissue sections (thickness will be determined, somewhere > between 20 and 300 um), and punching the particular regions I need out of > the sections. I will then isolate RNA from the punches for qPCR analysis. > > Questions: > > 1) does this sound like a viable plan? > 2) and suggestions, what to be careful of? > 3) where do I have to be careful of Rnase, should I use disposable blades, > cleaned with Rnase away? > 4) where can I find Rnase free slides, or should I just make my own. I > usually use charged slides. > > Any and all suggestions will be appreciated. I'm new to this and don't know > where I will have problems. > > Thanks! > > Caroline > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] used histology equipment
Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] used histology equipment
Does anyone know of a reputable dealer for used equipment? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gout Crytals
REMOVE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, July 06, 2010 3:50 PM To: Behnaz Sohrab Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Gout Crytals Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-boun...@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Schmitz, Sandy is out of the office.
I will be out of the office starting 07/05/2010 and will not return until 07/07/2010. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] bat wing histology
Thank you everyone for replying, much appreciated. Having also talked to people in my dept, I have a rudimentary protocol ready. Of course, I will have to tweak it to see what works. I will be using anti PGP9.5 antibody for neuronal immunology. I am still not sure how to section the wing. In most likelihood, I will be using freezing sliding microtome. The "swiss roll" method sound good and I will definitely try it. I am thinking that since the wing membrane is thin (~30 um), I will also try to use the whole mount of small pieces. Any other thoughts and advice would be appreciated. Thank you, Mohit Chadha, Univ of Maryland. On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks wrote: > Hi, > I do hope you are looking at cross sections of the wing and not the > flat. That would be very difficult indeed. For good cross sections I would > try a "Swiss Roll". This is a way of demonstrating a large amount of cross > sectional area in small space. Take the membrane and fix it by submersion in > the fixative of your choice. Prior to processing roll the whole membrane up > then cut the membrane log into sections small enough to fit in a cassette. > You can use foam biopsy pads to support this shape. Embed it and section it > on edge to show a long coiled membrane. The hairs should be able to be > displayed in this way as well. To show a lot of membrane at the same time > you could place multiple rolls in one cassette. This should work well. > > Good Luck, > Amos > > > Message: 21 > Date: Thu, 1 Jul 2010 11:46:17 -0400 > From: Mohit Chadha > Subject: [Histonet] bat wing histology > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everyone, > > This is my very first post and I am desperately looking for help. I am new > to histology, so any help would be much appreciated. > > I am studying the peripheral sensory innervation of bat wings. As a first > step, I would like to demonstrate the innervation pattern on the different > parts of the wing membrane (a whole mount of the wing?). Second, I would > like to demonstrate the mechanoreceptor make-up of the tiny hairs on the > wing membrane. > > Bat wings are highly elastic, with numerous folds, a thickness of about > 35-45 microns (in the species I study), a network of thin collagen bundles, > and pigmented superficial epidermal layers. > I could provide more information if required. > > Hoping to hear back from the members. > Thank you. > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gout Crytals
This works for us. Process normally, we usually fix in 100% alcohol. Cut slide Formula 83 or Xylene 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Eosin for 20 seconds 100% alcohol 20 seconds 100% alcohol 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Form 83 or Xylene for 20 seconds coverslip normally Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab [sohra...@ah.org] Sent: Tuesday, July 06, 2010 4:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gout Crytals
If you have fixed in formalin the gout crystals are all gone&
nbsp; Have to start over with a new sample if possible. The crystals
= dissolve in water, they should be fixed in 100% alcohol.
Sarah Goebel, B.A.= , HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin,= Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] Gout Crytals
From: "Behnaz Sohrab" <[1]sohra...@ah.= org>
Date: Tue, July 06, 2010 1:12 pm
To: <[2]histo...@lis= ts.utsouthwestern.edu>
Please refresh my memory!! Processing For Gout Crystal !1 Do I skip
all alc= ohols? Tissue has been fixed in formalin.?
Thank you
___
Histonet mailing list
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References
1. 3D"mailto://sohra...@ah.org"/
2. 3D"mailto://histonet@lists.utsouthwestern.edu"/
3. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
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Re: [Histonet] Gout Crytals
Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" Sent by: histonet-boun...@lists.utsouthwestern.edu 07/06/2010 01:28 PM To cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gout Crytals
Should skip the formalin!!! They are water soluable... :>( -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, July 06, 2010 16:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gout Crytals
Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rnase free slides?
Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] universal microtome aligner tool
Yes, I just seen that too. Would like information too if anyone has any. Thanks Kathy Gorham H.T. Grande Ronde Hospital La Grande, Or 9780 GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, July 06, 2010 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] universal microtome aligner tool Has anyone used the universal microtome aligner tool from Newcomer Supply? It is universal and much less expensive than most I have seen. I am just looking for some feedback. Thanks Nancy Schmitt HT, MLT (ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet- Xylene Substitutes
Vanessa, I work with a lot of great histology folks and what I know about it is that eosin bleeding and blue-haze problems usually occur as a consequence of either not fully removing all of the water during the final absolute alcohol rinse step, and/or not fully removing all of the alcohol during the clearing step. If the water and alcohol are completely removed, bleeding and haze will not occur. I hope this helps. Beth Austin-Sell CBG Biotech -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: control tissue for Hall's bile stain (Robert Richmond) 2. RE: BILLING QUESTION (Cynthia Pyse) 3. RE: ER and PR validation (jmye...@aol.com) 4. Thomas Crowell is out of the office. (thomas.crow...@novartis.com) 5. Looking for MIN6 cell line (Debra Baluch) 6. bat wing histology (Amos Brooks) 7. RE: BILLING QUESTION (Feher, Stephen) 8. xylene substitute (Vanessa Avalos) -- Message: 1 Date: Fri, 2 Jul 2010 13:48:52 -0400 From: Robert Richmond Subject: [Histonet] Re: control tissue for Hall's bile stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hall's bile stain - not really a stain but a reaction, like the Perls prussian blue reaction for iron - uses trichloracetic acid and ferric chloride to oxidize yellow-brown bilirubin to bright green biliverdin. Or so I've been told - I've never seen one. Something I've wondered about for a long time: could Hall's reaction be used to demonstrate meconium in macrophages in the chorion of the human placenta? These can be hard to see, particularly if you don't have a very good microscope. - If this would work, it would provide a ready source for controls, at least for the hospital pathologist. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 2 Date: Fri, 2 Jul 2010 13:51:51 -0400 From: "Cynthia Pyse" Subject: RE: [Histonet] BILLING QUESTION To: "'Sara Baldwin/mhhcc.org'" , Message-ID: <01cb1a0f$40ca1c10$c25e54...@com> Content-Type: text/plain; charset="us-ascii" We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Fri, 2 Jul 2010 13:54:26 EDT From: jmye...@aol.com Subject: RE: [Histonet] ER and PR validation To: histonet@lists.utsouthwestern.edu Cc: dorothy.l.w...@healthpartners.com Message-ID: <8f84.3cbd32f0.395f8...@aol.com> Content-Type: text/plain; charset="US-ASCII" This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe -- Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: "Webb, Dorothy L" Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent article written by 4 pathologists, including Dr. Elizabeth Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validate
[Histonet] universal microtome aligner tool
Has anyone used the universal microtome aligner tool from Newcomer Supply? It is universal and much less expensive than most I have seen. I am just looking for some feedback. Thanks Nancy Schmitt HT, MLT (ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 访问我的Netlog个人主页
嗨, 我创建了一个Ｎｅｔｌｏｇ个人主页，其中包括我的图片、视频、博客和活动。非常希望邀请你成为我的朋友，一起共享我们的天地。这需要你先在Ｎｅｔｌｏｇ上注册哦！在登录后，你也可以创建属于自己的个人主页了。 看一看: http://zh.netlog.com/go/mailurl/-bT0xMzQ2MDU0NDMmbD0xJmdtPTM3JnU9JTJGZ28lMkZyZWdpc3RlciUyRmlkJTNEOTk5OTU0NTk5JTI2aSUzRHQ5MQ__ 祝好, 沈淑琼 如果你不想再接接收到来自朋友的任何邀请，则请 http://zh.netlog.com/go/mailurl/-bT0xMzQ2MDU0NDMmbD0yJmdtPTM3JnU9JTJGZ28lMkZub21haWxzJTJGaW52aXRlJTJGZW1haWwlM0QtYUdsemRHOXVaWFJBYkdsemRITXVkWFJ6YjNWMGFIZGxjM1JsY200dVpXUjElMjZjb2RlJTNEMTMzNTU0NTAlMjZpZCUzRDk5OTk1NDU5OSUyNmklM0R0OTI_ Don't want to receive invitations from your friends anymore? http://zh.netlog.com/go/mailurl/-bT0xMzQ2MDU0NDMmbD0zJmdtPTM3JnU9aHR0cCUzQSUyRiUyRmVuLm5ldGxvZy5jb20lMkZnbyUyRm5vbWFpbHMlMkZpbnZpdGUlMkZlbWFpbCUzRC1hR2x6ZEc5dVpYUkFiR2x6ZEhNdWRYUnpiM1YwYUhkbGMzUmxjbTR1WldSMSUyNmNvZGUlM0QxMzM1NTQ1MCUyNmlkJTNEOTk5OTU0NTk5JTI2aSUzRHQ5Mg__ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Unsubscribe
Unsubscribe please. Thanks, Jeff Oliver Services Lab Specialist II Asterand, Inc 440 Burroughs Detroit, Michigan 48202 phone: 313-263-0960 fax:313-263-0961 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Turtox Internal Ear, cochlea slides
Hi Michelle, I worked for a company called WARD'S Natural Science in Rochester, NY; they produce/provide science education materials. I can't say I ever produced the internal ear slide myself, but I can recall others in the department working on something similar. I would give them a call at 1-800-962-2660 and ask to speak with a tech or supervisor in the microscope slides department. Even if they don't normally carry what you're looking for, I am sure they would be happy to try and provide you with what you want. I hope you find what you're looking for! Best of luck. Adrienne Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Sunday, July 4, 2010 1:45:10 PM Subject: Histonet Digest, Vol 80, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides (Patsy Ruegg) 2. RE: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks (Patsy Ruegg) 3. RE: SPAM-LOW: RE: [Histonet] Canine bone sectioning trouble (Patsy Ruegg) 4. RE: SPAM-LOW: Re: [Histonet] Unstained Slides (Patsy Ruegg) -- Message: 1 Date: Sun, 4 Jul 2010 10:42:47 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides To: "'Atoska Gentry'" , Message-ID: <7568a26877eb4c92bd7f83fd0c984...@prueggihctechlt> Content-Type: text/plain; charset="us-ascii" Wow that is going to be a tuff one, back in the day I tried to cut Petris pyramid from the inter ear and was told it was the densest/hardest bone in the body and it sure seemed to be. Even decalcified we had to embed it in plastic and use permanent tungsten carbide D profile knives to cut any sections. I am not at the university anymore so can't access those slides or I would try to help you out. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Thursday, July 01, 2010 10:24 AM To: histo...@pathology.swmed.edu Subject: SPAM-LOW: [Histonet] Re: replacement Turtox Internal Ear, cochlea slides Hello! please see below inquiry from a colleague. Thanks! Atoska Our histology class is looking for replacements for old Turtox (Guinea Pig) Internal Ear, cochlea slides and was wondering if anyone knew a company who supplied these slides or tissue blocks? I don't think it has to be guinea pig but I haven't been able to locate internal ear slides from any species. Any help would be appreciated. Thanks! ~~~ Michelle (Shelly) Aono Histology Technician IV CVM & AU Staff Advisory Council Representative Auburn University CVM-APP 212 Greene Hall, Auburn, AL 36832 (334) 844-5594 -- Message: 2 Date: Sun, 4 Jul 2010 10:46:25 -0600 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks To: "'Kim Merriam'" , "'Sherwood, Margaret'" , "'Jay Lundgren'" , "'Laurie Colbert'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <8178eeb0db06440ab8a5250a81e75...@prueggihctechlt> Content-Type: text/plain; charset="iso-8859-1" Yep that is what I do, I use the plastic containers I get salad or other stuff in I would throw away, fill it and freeze it, I add some water to it and put the blocks on the frozen tray and they get cold and hydrated at the same time. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Thursday, July 01, 2010 6:38 AM To: Sherwood, Margaret; Jay Lundgren; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Chilling Tray for Blocks we use something like this: https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=414004-256&in E=1&highlight=414004-256 we keep several sizes in the freezer, depending on�how many blocks we need to cut.� The nice thing is that it is cheap and you can hydrate your blocks at the same time you are chilling them. Kim �Kim Merriam, MA,
