Re: [Histonet] Rnase free slides?
Hi Emily, As you said RNAases are everywhere, so chasing them all down would be akin to hunting the boogyman. The way I see it is since there is no realistic way of eliminating any contact at all with RNAase it really comes down to merely damage control. Start with gloves, you reduce the introduction of RNAase by a certain percentage. Wipe down all the surfaces that come in contact with the tissue with RNAase away, you reduced the percentage a bit further. Only use DEPC treated water and you reduce the percentage further and so on until it gets to be so labor intensive that it is non productive. You can hold your breath to reduce them too... don't laugh I found myself doing it unwittingly one day. So what happens if we scale it down to a workable level? Some RNAases will inevitably be introduced this will reduce the PCR yield some. If you are doing it a certain way and your yields are sufficient, it aint broke so don't fix it! So if you are using untreated slides and it's working, don't change a thing. That having been said I never assume anything is RNAase free. This includes the slides and any chemicals I use, previously opened or not. So if it doesn't take much effort to RNAase away treat something I usually do it. Otherwise I just chalk it up to acceptable yield loss. Incidentally I meant 30% sucrose when I said 30% in my last post. I've never done a side by side comparison of the difference between 30% sucrose in DEPC and OCT. There may be no appreciable difference at all. I merely mentioned it as an option. So a funny story: I had a researcher really giving me H-E-double hockey sticks wanting me to make absolutly sure there was no possibility of RNAase contamination. (Thus the holding my breath I mentioned earlier.) So I went into full OCD mode and decontaminated everything in RNAase away including the box I put the slides in. The researcher picked up the slides and after grilling me again about my technique he popped open the box (bare-handed, mind you) to check the slides out and ran his finger down the sides of the slides. I literally saw the RNAase bugs eating away all my hard work before my eyes. When I caught my breath I took a long lunch and went home early. Later that week I saw the researcher. He said the yield was great and I must have followed his advise well. That was a Scotch night! RNAase ... the boogeyman and Big Foot! Happy Wednesday, Amos On Wed, Jul 7, 2010 at 7:32 PM, Emily Sours wrote: > Am I wrong in assuming that the glass slides I get (which are in a > plastic box, wrapped in plastic) are not RNase free? They're > superfrost plus from Fisher. It hasn't been a problem for us to > assume so, but I'm wondering what other labs think about it. > We've never treated our slides with Rnase Away or rinsed in DEPC > water. We use DEPC water for in situ hybridization solutions. The in > situ dishes are sprayed with RNase away, then DEPC water. > Also use OCT when you section, it'll make your life so much easier! > It's assumed in my lab that if a chemical came in and hasn't been > opened, it's RNase free. To keep it RNase free, you always wear > gloves. Store your slides in a new box to keep them RNase free. > > RNases are everywhere on people, I assumed, and that was the problem. > I didn't think RNases are everywhere in the universe on every object. > But I could be wrong. Anyone else have an opinion? > > Emily > -- > Dark Pictures, thrones and stones that pilgrims kiss > And poems that take a thousand years to die > But ape the immortality of this > Red label on a little butterfly. > > -Vladimir Nabokov, concluding stanza of ‘A Discovery’ 1941. > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] DAB
Keri, For the free base of DAB you will need to dissolve it in N,N dimethyl formamide. The free base is poorly soluble in aqueous solutions. Substitute the formamide for the distilled water: 1. Weigh out 2g DAB in a fume hood 2. Dissolve in 10ml N,N dimethyl formamide 3. Label fifty 1ml reagent vials 4. Aliquot 0.1ml DAB solution in each tube 5. Freeze and Store at -20oC For use: Add 0.1ml DAB solution to 50ml buffer and add 50 microlitres of 30% hydrogen peroxide. I prefer to use the DAB tetrahydrochloride Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Thursday, 8 July 2010 7:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB Hello Histonetters, I am currently trying to work out a new DAB protocol to be used with IHC on FFPE sections. I am testing three different protocols, and am having limited success. The first protocol requires me to make a DAB stock solution of 10mg in 1 ml dH20, which is then filtered and frozen immediately. This protocol suggests using DAB tetrahydrochloride XH20, but I am using the free base of DAB as that is what was on hand. The free base does not want to go into solution, but has produced some reaction in my IHC. My second protocol asks for 40mg DAB in 1.5 ml Tris buffer (0.05M, pH 7.6), to make my stock solution. I am again using the free base, and have been stirring since 11:30 am yesterday (MDT) with the result being a chocolate milk-like substance. My third protocol requires me to make up the DAB fresh each time I want to use it. This protocol also asks for the tetrahydrochloride form, but again I used the free base and did get some reaction. Does anyone have thoughts on about how to get the free base to go into solution without oxidizing, or thoughts about free base versus the other form of DAB? Thanks! Keri Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colw...@inspection.gc.ca Telephone | Téléphone: 403-382-5500 Facsimile | Télécopieur: 403-382-5583 Government of Canada | Gouvernement du Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Reprocess
You do not need to rehydrate the blocks back to water, While the tissue is still hot (ie the wax is still molten) blot the tissue dry, place it back in its cassette and place the cassette in 10% formalin for reprocessing. This procedure dramatically improves the results in at least 90% of cases. The excellent results are probably due to the protective nature of the wax present in the adequately processed portions of the block. This insulates the tissue from the harmful effects of ethanol on the adequately processed portions of the tissue preventing the tissue from becoming hard and brittle (Johnson 2003). Johnson (2003) Histologic 36(1):21-22. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Thursday, 8 July 2010 1:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =oday is...I grossed in some fat that I need to routine process. I ha=e done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =the middle of my block. I fixed for 48 hours, but guess my section we=re too big and needed more. I want to fix for a little longer and rep=ocess the block. I know I need to melt it down, but then what? =I have done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=SCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Rnase free slides?
Am I wrong in assuming that the glass slides I get (which are in a plastic box, wrapped in plastic) are not RNase free? They're superfrost plus from Fisher. It hasn't been a problem for us to assume so, but I'm wondering what other labs think about it. We've never treated our slides with Rnase Away or rinsed in DEPC water. We use DEPC water for in situ hybridization solutions. The in situ dishes are sprayed with RNase away, then DEPC water. Also use OCT when you section, it'll make your life so much easier! It's assumed in my lab that if a chemical came in and hasn't been opened, it's RNase free. To keep it RNase free, you always wear gloves. Store your slides in a new box to keep them RNase free. RNases are everywhere on people, I assumed, and that was the problem. I didn't think RNases are everywhere in the universe on every object. But I could be wrong. Anyone else have an opinion? Emily -- Dark Pictures, thrones and stones that pilgrims kiss And poems that take a thousand years to die But ape the immortality of this Red label on a little butterfly. -Vladimir Nabokov, concluding stanza of ‘A Discovery’ 1941. On Wed, Jul 7, 2010 at 5:13 PM, Amos Brooks wrote: > Hi, > This is certainly possible, but RNAase is everywhere, and in a cryostat > you really can't remove it because the RNA remover is an aqueous solution > that will freeze in the cryostat. A disposable blade (RNAase cleaned) is > probably the best you can do there. One thing to consider, I'm sure OCT > isn't RNAase free, so perhaps you should use 30% in DEPC treated water. > I have not seen RNAase free slides being sold by any vendors. I usually > use LCM slides, but your are doing this a bit differently. For this I > wouldn't waste my money on buying RNAase free slides. You can just dip the > slides in RNAase away and let them air dry (use them soon after though). > Further, I would not use charged slides as that would be counter-productive. > You are trying to get tissues off the slides, so adhesives would not be > helpful to you. Just a plain ole' glass slide with no bells or whistles. > Come to think of it that may be hard to find too ;-) > > Good luck, > Amos > > > Message: 1 > Date: Tue, 06 Jul 2010 15:49:58 -0400 > From: Caroline Bass > Subject: [Histonet] Rnase free slides? > To: > Message-ID: >> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > > I'm doing RNA work for the first time. My plan is to take a fresh rat brain, > block quickly, freeze by immersing in dry-ice cooled isopentane, storing at > -80, collecting tissue sections (thickness will be determined, somewhere > between 20 and 300 um), and punching the particular regions I need out of > the sections. I will then isolate RNA from the punches for qPCR analysis. > > Questions: > > 1) does this sound like a viable plan? > 2) and suggestions, what to be careful of? > 3) where do I have to be careful of Rnase, should I use disposable blades, > cleaned with Rnase away? > 4) where can I find Rnase free slides, or should I just make my own. I > usually use charged slides. > > Any and all suggestions will be appreciated. I'm new to this and don't know > where I will have problems. > > Thanks! > > Caroline > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: gout crystals
Gout crystals are monosodium urate. In clinical material they're found
in large masses, called tophi (TOE-fie, singular tophus). The
individual crystals are long and needle-like, with negative
birefringence if your pathologist is so fortunate as to have a full
wave plate (a.k.a. first order plate, gout slider) on their
microscope.
The alcohol fixation and processing routine is ideal, but rarely
achievable. The specimen will normally come to you in formalin. The
crystal masses in the tophi can be picked out with the point of a
scalpel blade (if you're still allowed scalpel blades with points on
them) into water or alcohol, and looked at as a wet preparation in a
polarizing microscope, where you can see the crystals with the aid of
a polarizer. Calcium pyrophosphate ("pseudogout") crystals, blocky
with positive birefringence, can also be identified with this
technique.
The CPT code for crystal examination cannot, I think, be assigned in
addition to the 88305.
Bob Richmond
Samurai Pathologist
Knoxville TN
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[Histonet] universal microtome aligner
We purchased this item from Newcomer supply and have been universally disappointed in it. The concept for the tool is good, but we have found that it will not clamp tightly onto our Leica microtomes (model RM2125). Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Histology position in the Seattle Washington area
Hello Netters! I am trying to relocate to the Seattle Washington area and looking for a Histology position. I have 17 years experience, have set up labs as consultant and also have 5 years managememt experience. Will consider any position. I am also HT ascp. Thank you in advance!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rnase free slides?
Hi, This is certainly possible, but RNAase is everywhere, and in a cryostat you really can't remove it because the RNA remover is an aqueous solution that will freeze in the cryostat. A disposable blade (RNAase cleaned) is probably the best you can do there. One thing to consider, I'm sure OCT isn't RNAase free, so perhaps you should use 30% in DEPC treated water. I have not seen RNAase free slides being sold by any vendors. I usually use LCM slides, but your are doing this a bit differently. For this I wouldn't waste my money on buying RNAase free slides. You can just dip the slides in RNAase away and let them air dry (use them soon after though). Further, I would not use charged slides as that would be counter-productive. You are trying to get tissues off the slides, so adhesives would not be helpful to you. Just a plain ole' glass slide with no bells or whistles. Come to think of it that may be hard to find too ;-) Good luck, Amos Message: 1 Date: Tue, 06 Jul 2010 15:49:58 -0400 From: Caroline Bass Subject: [Histonet] Rnase free slides? To: Message-ID: > Content-Type: text/plain; charset="US-ASCII" Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB
Hello Histonetters, I am currently trying to work out a new DAB protocol to be used with IHC on FFPE sections. I am testing three different protocols, and am having limited success. The first protocol requires me to make a DAB stock solution of 10mg in 1 ml dH20, which is then filtered and frozen immediately. This protocol suggests using DAB tetrahydrochloride XH20, but I am using the free base of DAB as that is what was on hand. The free base does not want to go into solution, but has produced some reaction in my IHC. My second protocol asks for 40mg DAB in 1.5 ml Tris buffer (0.05M, pH 7.6), to make my stock solution. I am again using the free base, and have been stirring since 11:30 am yesterday (MDT) with the result being a chocolate milk-like substance. My third protocol requires me to make up the DAB fresh each time I want to use it. This protocol also asks for the tetrahydrochloride form, but again I used the free base and did get some reaction. Does anyone have thoughts on about how to get the free base to go into solution without oxidizing, or thoughts about free base versus the other form of DAB? Thanks! Keri Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colw...@inspection.gc.ca Telephone | Téléphone: 403-382-5500 Facsimile | Télécopieur: 403-382-5583 Government of Canada | Gouvernement du Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Has anyone used a biotin block in their antibody diluent?
I assume that you are using a biotinylated detection system? If so, endogenous biotin labelling is specific, tho' NOT wanted ;-) So, before you try variations, just buy a kit and see if your "non-specific" immunostaining is due to endog. biotin ...follow their instructions by pre-incubating in the kit. Then, if you find that the staining you get is due to endog. biotin...you can modify away ( such as including in primary Ab: However, the kits are RTU: so, by adding to primary Ab, for eg, you use a heck of a lot of kit solution.;-) I always pre-incubate because there are so many tissues that do NOT require endog. biotin blocking. Also, if you discover that endogenous biotin is the problem and you will therefore need to use a biotin blocking kit regularly, why not make up your own? Far cheaper/equally effective: many sites give protocols, such as this one http://www.immunoportal.com/ Please Post further if you have any problems. Good luck. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Reprocess
Is this run backwards ok? I want to keep the fat. In = fact I only
want the fat?
Sarah Goebel, B.A., HT (ASCP)
Histot= echnician
XBiotech USA Inc.
8201 East Riversi= de Dr. Bldg 4 Suite 100
Austin, Texas 78744<= /div>
(512)386-5107
Original Message
Subject: RE: [Histonet] Reprocess
From: Cheri Miller <[1]cmil...@phy= slab.com>
Date: Wed, July 07, 2010 8:53 am
To:"[2]sgoe...@xbiotech.com"&l=t;[3]sgoe...@xbiotech.com>,
"[4]histo...@lists.utso= uthwestern.edu"
<[5]histo...@lists.utsouthwestern.edu>
Hi Sarah, I don't melt them down I just drop them in with my dirty
molds an= d lids and run them through the cleaning cycle on my
processor. The Xylene = and Alcohol will melt the oily fat. When the
clean cycle is complete I just= drop them back into formalin until I
process that evening. It works very w= ell.
Cheryl A. Miller HT(ASAP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554
-Original Message-
From: [6]histon= et-boun...@lists.utsouthwestern.edu
[[7]mailto:histonet-boun...@lists.utsouthwestern.edu= ] On Behalf Of
[8]sgoe...@xbiote= ch.com
Sent: Wednesday, July 07, 2010 10:37 AM
To: [9]histo...@lists.u= tsouthwestern.edu
Subject: [Histonet] Reprocess
Hello all!! Hope everyone had a happy 4th!! Question =day is...I
grossed in some fat that I need to routine process. I ha= done
this in the past with extra fixation and no problem? This tim=
however it didn't fix all the way through and I have oily unfixed fat
in =e middle of my block. I fixed for 48 hours, but guess my
section we= too big and needed more. I want to fix for a little
longer and rep=cess the block. I know I need to melt it down, but
then what? =ave done this before, it's just my brain is getting
older =)
Thanks in advance!!
Sarah Goebel, B.A., HT (=CP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
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information. Any dissemination, distr= ibution, or copying of this
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from your system.
References
1. 3D"mailto://cmil...@physlab.com"/
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RE: [Histonet] (no subject)
I totally agree!!! Why did I spend the time, effort, and mo= ney
(additionally to stay certified) if I am not going to get a pay
incenti= ve to do so? Sure, anyone can get OJT for many things, but
would you = pay a non-registered PA (pathologist assistant) the same
as someone who had= completed a masters program and passed the
exam...of course not (in fact y= ou probably wouldn't even hire the
person!).I have worked in places = where there are these
"non-registered histotechs", my question always was..= .what even is
this position? I had to pay my dues as an underpaid lab= assistant
like I'm sure everyone else did while getting my degree and regi
stry...why shouldn't everyone else? I know in the past it really
gets= under people's skin when non-registered are making only $2 less
an hour th= an a registered tech with the same experience. I would
say listen to = your "dinosaur" management...you have to earn the
money you make!!!
Sarah G= oebel, B.A., HT (ASCP)
Histotechnician
XBiote= ch USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] (no subject)
From: "Gunderson, Michael" <[1]m= gund...@fairview.org>
Date: Wed, July 07, 2010 10:31 am
To: Jackie M O'Connor , "Fredrickson,
Mona"
<[3]mfredrick...@nrh-ok.com>
Cc: "[4]histo...@lists.= utsouthwestern.edu"
<[5]histo...@lists.u= tsouthwestern.edu>,
"[6]histonet-bo= un...@lists.utsouthwestern.edu"
<[7]histonet= -boun...@lists.utsouthwestern.edu>
By telling someone they should earn the same without passing our
registrati= on exam diminishes the effort and hard work of registered
HT and HTL's. Ma= nagement are not dinosaurs, they are fair. The exam
is there to weed out = the weak and under or un-qualified, if you
cannot pass the test, you have n= ot earned the right of those who
have.
Michael A. Gunderson HTL(ASCP)
Lead Technologist-Immunostains Laboratory
University of Minnesota Medical Center-Fairview
2450 Riverside Avenue
Minneapolis, MN 55454
Laboratory: 1-612-273-9119
Fax: 1-612-273-4879
Email: [8]mgund...@fairview.org
From: [9]histon= et-boun...@lists.utsouthwestern.edu
[[10]histonet-boun...@lists.utsouthwestern.edu]= On Behalf Of Jackie M
O'Connor [Jackie.O'[11]con...@abbott.com]
Sent: Wednesday, July 07, 2010 12:09 PM
To: Fredrickson, Mona
Cc: [12]histo...@lists.u= tsouthwestern.edu;
[13]histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)
Ask management if he would suddenly be a better technician and more
valuable if he passed the exam? Duh - no. In my opinion, merit
increases should be based on merit - not documentation.
If he's not eligible, he's not eligible. Does your management increase
pay based on certification? So, if a certified tech with one year of
experience came to your lab, they would be paid more than an incumbent
tech with 8 years experience? Sounds like you have some dinosaur
management there.
From:
"Fredrickson, Mona" <[14]mfredr= ick...@nrh-ok.com>
To:
"[15]histo...@lists.utso= uthwestern.edu"
<[16]histo...@lists.utsouthwestern.edu>
Date:
07/07/2010 12:04 PM
Subject:
[Histonet] (no subject)
Sent by:
[17]histonet-bou= n...@lists.utsouthwestern.edu
Hello All in Histoland,
I have a tech who is employed as Histotech eligible, but he was not
able
to pass the HT exam and now is no longer eligible to thake the test
because he has to get his associates degree in science. The lab
management wants to change his title from histotech eligible to
histotech non-registered without pay increase. But I feel the pay
should be
increased. So I would appreciate comments on the following:
1.) should title be changed from eligible to non-registered?
2.) After having done this for 8 years should his pay stay the same?
3. Should job responsibilities remain the same
Thank you in advance for feedback!
Histotech in Oklahoma
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RE: [Histonet] (no subject)
He did not meet his and his employers agreed stipulations for employment. If he was being paid equal to that of a registered tech then he should receive a decrease in pay. If not then he should continue with merit increases only. Getting an ASCP registry is a marketable achievement for both the tech and the employer. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
It seems to me that Histotech non-registered without pay increase would be appropriate. Why would you change his title and give him an increase in pay when he didn't fulfill his employment agreement? I would not decrease his pay and I would not terminate a good employee. Apparently he earned his present pay as an "undocumented" non-registered Histotech and nothing has changed. He should continue to get merit increases as per usual, with the goal of getting the Associates Degree, and registry (if still interested). He should be allowed to perform any function he has been deemed competent to perform. Just my 2 cents. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net "Fredrickson, Mona" Sent by: histonet-boun...@lists.utsouthwestern.edu 07/07/2010 01:04 PM To "Histonet@lists.utsouthwestern.edu" cc Subject [Histonet] (no subject) Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
I have known some really crummy MD's too but I still wouldn't want to go to a physician that couldn't pass his boards. I sympathize with the person that didn't pass the exam and that doesn't make him a poor histotech just not one that gets the HT (ASCP) after his name and not eligible for the same pay scale as that of a registered tech. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, July 07, 2010 1:35 PM To: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Fredrickson, Mona Subject: RE: [Histonet] (no subject) Over the years, I have had some HT and HTL registered technologists who were crummy technicians. I'm jus' sayin' that certification doesn't automatically mean you are better than a non-certified tech. From: "Gunderson, Michael" To: Jackie M O'Connor , "Fredrickson, Mona" Cc: "Histonet@lists.utsouthwestern.edu" , "histonet-boun...@lists.utsouthwestern.edu" Date: 07/07/2010 12:31 PM Subject: RE: [Histonet] (no subject) By telling someone they should earn the same without passing our registration exam diminishes the effort and hard work of registered HT and HTL's. Management are not dinosaurs, they are fair. The exam is there to weed out the weak and under or un-qualified, if you cannot pass the test, you have not earned the right of those who have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: mgund...@fairview.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor [Jackie.O'con...@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I agree with Tom and Michael. Those who have been fortunate enough to pass deserve the kudos and pay increase. As for the title, he simply isn't eligible at this point. He is a non-registered tech. Sheila Haas Laboratory Supervisor Micro Path Laboratories From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Sent: Wed, July 7, 2010 1:03:55 PM Subject: [Histonet] (no subject) Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Over the years, I have had some HT and HTL registered technologists who were crummy technicians. I'm jus' sayin' that certification doesn't automatically mean you are better than a non-certified tech. From: "Gunderson, Michael" To: Jackie M O'Connor , "Fredrickson, Mona" Cc: "Histonet@lists.utsouthwestern.edu" , "histonet-boun...@lists.utsouthwestern.edu" Date: 07/07/2010 12:31 PM Subject: RE: [Histonet] (no subject) By telling someone they should earn the same without passing our registration exam diminishes the effort and hard work of registered HT and HTL's. Management are not dinosaurs, they are fair. The exam is there to weed out the weak and under or un-qualified, if you cannot pass the test, you have not earned the right of those who have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: mgund...@fairview.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor [Jackie.O'con...@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
By telling someone they should earn the same without passing our registration exam diminishes the effort and hard work of registered HT and HTL's. Management are not dinosaurs, they are fair. The exam is there to weed out the weak and under or un-qualified, if you cannot pass the test, you have not earned the right of those who have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: mgund...@fairview.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor [Jackie.O'con...@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QC log
I forgot to say that we use a different one for specials so if you need
that also let me know.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gill,
Caula A.
Sent: Wednesday, July 07, 2010 1:19 PM
To: Scott, Allison D; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] QC log
This is the QC sheet we use and the Pathologist documents any issues
there may be if any.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, July 07, 2010 12:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] QC log
Hello to all in histoland. Does anyone have a QC log sheet that
documents the H&E stain, microtomy, floaters and other issues that they
would be willing to share with me. Any help in this matter will be
greatly appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
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To the extent the information in this e-mail and any attachments contain
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Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR
Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is
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RE: [Histonet] (no subject)
That is a hard question. Here when we hire a un registered tech, they are given a time limit (3 years) to get their certification. At the end of three years, we reserve the right to terminate if they have not achieved their certification. What are the levels for your lab? Do you have anything like Lab Scientist 1, 2 or 3 and if so are there pay ranges between each level? If there are levels like that, I as manager, would keep him in level 1 with no salary increase until the degree is finished. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fredrickson, Mona [mfredrick...@nrh-ok.com] Sent: Wednesday, July 07, 2010 1:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QC log
This is the QC sheet we use and the Pathologist documents any issues
there may be if any.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, July 07, 2010 12:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] QC log
Hello to all in histoland. Does anyone have a QC log sheet that
documents the H&E stain, microtomy, floaters and other issues that they
would be willing to share with me. Any help in this matter will be
greatly appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
your computer system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance
Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR
Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is
confidential and/or privileged. This e-mail may also be confidential
and/or privileged under Texas law. The e-mail is for the use of only
the individual or entity named above. If you are not the intended
recipient, or any authorized representative of the intended recipient,
you are hereby notified that any review, dissemination or copying of
this e-mail and its attachments is strictly prohibited.
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Re: [Histonet] (no subject)
Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: "Fredrickson, Mona" To: "Histonet@lists.utsouthwestern.edu" Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: gout crystals
See these articles- keep formalin fixation to <12 hrs. Vinod Shidham, Ganesh Shidham (2000) Staining Method to Demonstrate Urate Crystals in Formalin-Fixed, Paraffin-Embedded Tissue Sections. Archives of Pathology & Laboratory Medicine: Vol. 124, No. 5, pp. 774-776. Shidham V, Chivukula M, Basir Z, Shidham G. Mod Pathol. 2001 Aug;14(8):806-10. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HOOD MS. GLENDA Sent: Wednesday, July 07, 2010 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: gout crystals It is true that gout crystals are water-soluble, and we are all taught that... but a few years ago there was a published article (JOH??) that showed that many crystals DO survive after water-based fixation. Since you already have the specimen in formalin, at least try processing and see if they can be demonstrated. Couldn't hurt, not nearly as much as the patient would for a re-biopsy! Glenda F. Hood, M.Ed., HT(ASCP) Instructor and Program Director Histotechnology Program Tarleton State University 817-926-1101 x6 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, July 07, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Rnase free slides? (Caroline Bass) 2. Gout Crytals (Behnaz Sohrab) 3. RE: Gout Crytals (Weems, Joyce) 4. Re: Gout Crytals (Jennifer MacDonald) 5. RE: Gout Crytals (sgoe...@xbiotech.com) 6. Schmitz, Sandy is out of the office. (sandy.schm...@leica-microsystems.com) 7. RE: Gout Crytals (Joyce Cline) 8. Re: bat wing histology (Mohit Chadha) 9. RE: Gout Crytals (Douglas,Joseph) 10. used histology equipment (dcoj...@tampabay.rr.com) 11. Re: used histology equipment (Drew Meyer) 12. Re: Rnase free slides? (Emily Sours) 13. Re: bat wing histology (Amos Brooks) 14. Has anyone used a biotin block in their antibody diluent? (Jennifer Campbell) 15. Biotin block (Jim Reilly) 16. RE: Has anyone used a biotin block in their antibody diluent? (Mauger, Joanne) 17. Re: used histology equipment (kim.dona...@bhcpns.org) 18. RE: used histology equipment (Douglas,Joseph) 19. RE: used histology equipment (Douglas,Joseph) 20. Reprocess (sgoe...@xbiotech.com) 21. Re: Reprocess (Catherine Simonson) 22. RE: Reprocess (Cheri Miller) 23. RE: Reprocess (Cheri Miller) 24. RE: Reprocess (Mahoney,Janice A) -- Message: 1 Date: Tue, 06 Jul 2010 15:49:58 -0400 From: Caroline Bass Subject: [Histonet] Rnase free slides? To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline -- Message: 2 Date: Tue, 06 Jul 2010 13:12:49 -0700 From: "Behnaz Sohrab" Subject: [Histonet] Gout Crytals To: Message-ID: <4c332bd0.4347.005...@ah.org> Content-Type: text/plain; charset=US-ASCII Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you -- Message: 3 Date: Tue, 6 Jul 2010 16:35:41 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Gout Crytals To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <92ad9b20a6c3
RE: [Histonet] Reprocess
Cheri's process is a good one, we use it too. Works every time. Jan Mahoney Omaha, NE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: gout crystals
It is true that gout crystals are water-soluble, and we are all taught that... but a few years ago there was a published article (JOH??) that showed that many crystals DO survive after water-based fixation. Since you already have the specimen in formalin, at least try processing and see if they can be demonstrated. Couldn't hurt, not nearly as much as the patient would for a re-biopsy! Glenda F. Hood, M.Ed., HT(ASCP) Instructor and Program Director Histotechnology Program Tarleton State University 817-926-1101 x6 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, July 07, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Rnase free slides? (Caroline Bass) 2. Gout Crytals (Behnaz Sohrab) 3. RE: Gout Crytals (Weems, Joyce) 4. Re: Gout Crytals (Jennifer MacDonald) 5. RE: Gout Crytals (sgoe...@xbiotech.com) 6. Schmitz, Sandy is out of the office. (sandy.schm...@leica-microsystems.com) 7. RE: Gout Crytals (Joyce Cline) 8. Re: bat wing histology (Mohit Chadha) 9. RE: Gout Crytals (Douglas,Joseph) 10. used histology equipment (dcoj...@tampabay.rr.com) 11. Re: used histology equipment (Drew Meyer) 12. Re: Rnase free slides? (Emily Sours) 13. Re: bat wing histology (Amos Brooks) 14. Has anyone used a biotin block in their antibody diluent? (Jennifer Campbell) 15. Biotin block (Jim Reilly) 16. RE: Has anyone used a biotin block in their antibody diluent? (Mauger, Joanne) 17. Re: used histology equipment (kim.dona...@bhcpns.org) 18. RE: used histology equipment (Douglas,Joseph) 19. RE: used histology equipment (Douglas,Joseph) 20. Reprocess (sgoe...@xbiotech.com) 21. Re: Reprocess (Catherine Simonson) 22. RE: Reprocess (Cheri Miller) 23. RE: Reprocess (Cheri Miller) 24. RE: Reprocess (Mahoney,Janice A) -- Message: 1 Date: Tue, 06 Jul 2010 15:49:58 -0400 From: Caroline Bass Subject: [Histonet] Rnase free slides? To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline -- Message: 2 Date: Tue, 06 Jul 2010 13:12:49 -0700 From: "Behnaz Sohrab" Subject: [Histonet] Gout Crytals To: Message-ID: <4c332bd0.4347.005...@ah.org> Content-Type: text/plain; charset=US-ASCII Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you -- Message: 3 Date: Tue, 6 Jul 2010 16:35:41 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Gout Crytals To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <92ad9b20a6c38c4587a9febe3a30e164015e968...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Should skip the formalin!!! They are water soluable... :>( -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, July 06, 2010 16:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the
[Histonet] QC log
Hello to all in histoland. Does anyone have a QC log sheet that
documents the H&E stain, microtomy, floaters and other issues that they
would be willing to share with me. Any help in this matter will be
greatly appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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RE: [Histonet] Reprocess
I take that back I do melt them, recap the cassettes and then drop them in to clean. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, July 07, 2010 10:53 AM To: sgoe...@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocess Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Reprocess
Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Wednesday, July 07, 2010 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reprocess
Easy option is to melt down, and run cassettes through the clean cycle on your processors to remove all the paraffin. then process as usual. I've done this before with decent results. Good luck! Catherine On Wed, Jul 7, 2010 at 11:36 AM, wrote: > > Hello all!! Hope everyone had a happy 4th!! Question today is...I > grossed in some fat that I need to routine process. I ha ve done > this in the past with extra fixation and no problem? This tim e > however it didn't fix all the way through and I have oily unfixed fat > inthe middle of my block. I fixed for 48 hours, but guess my > section we re too big and needed more. I want to fix for a little > longer and rep rocess the block. I know I need to melt it down, but > then what? I have done this before, it's just my brain is getting > older =) > > Thanks in advance!! > > Sarah Goebel, B.A., HT ( ASCP) > > Histotechnician > > XBiotech USA Inc. > > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-5107 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reprocess
Hello all!! Hope everyone had a happy 4th!! Question = today is...I grossed in some fat that I need to routine process. I ha= ve done this in the past with extra fixation and no problem? This tim= e however it didn't fix all the way through and I have oily unfixed fat in = the middle of my block. I fixed for 48 hours, but guess my section we= re too big and needed more. I want to fix for a little longer and rep= rocess the block. I know I need to melt it down, but then what? = I have done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (= ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] used histology equipment
REMOVE FROM DATABASE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kim.dona...@bhcpns.org Sent: Wednesday, July 07, 2010 8:37 AM To: dcoj...@tampabay.rr.com Cc: 'histonet'; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] used histology equipment Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-boun...@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] used histology equipment
REMOVE FROM DATABASE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, July 06, 2010 5:56 PM To: dcoj...@tampabay.rr.com Cc: histonet Subject: Re: [Histonet] used histology equipment Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, wrote: > Does anyone know of a reputable dealer for used equipment? > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] used histology equipment
Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Sent by: histonet-boun...@lists.utsouthwestern.edu 07/06/2010 05:29 PM To "'histonet'" cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Has anyone used a biotin block in their antibody diluent?
Jennifer, Vector has an avidin-biotin blocking kit that works well by itself or mixed with reagents- very reasonable cost. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell [jcampb...@vdxpathology.com] Sent: Tuesday, July 06, 2010 10:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biotin block
Hello Jennifer I use the Avidin/Biotin blocking kit from Vector SP-2001 I mix the Avidin D with my normal blocking serum and the biotin I add to my primary antibody diluent. This seems to work well for most tissue types. Cheers Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
