[Histonet] Staining dish for vertical 30-slide rack

[Morken, Tim]

Does anyone know of a plastic, inexpensive staining dish for the Richard-Allan 
or Raymond Lamb 30-slide vertical rack. Something similar to the tissue tek 
plastic stain dish. The Rack is 11cm / 4 3/8" long


Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
Box 1656
1600 Divisadero St.
San Francisco, CA 94143-1656
USA

Phone: (415) 514-6042
Pager: (415) 443-6509
Fax: (415) 885-7409

Email: tim.mor...@ucsfmedctr.org

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Re: [Histonet] Sectioning problem

[Bryan Llewellyn]

I had spring problems on the block holder of a microtome many years ago.  I 
solved it by getting a local engineering shop to make me some new springs 
using the old springs as a template.  It worked fine, and was cheap, too.


Bryan Llewellyn


- Original Message - 
From: 

To: "histonet" 
Sent: Thursday, July 22, 2010 1:12 PM
Subject: [Histonet] Sectioning problem


I am working on an old microtome, a Reichert Histostat with a Tissue-Tek
II microtome chuck adapter, and the last time I had this machine cleaned
and rehabbed by my repairman, he said that the springs were shot on the
chuck adapter, resulting in the windowblind chatter that I was getting in
my sections. Unfortunately he did not have a replacement, Sakura doesn't
make or sell them anymore, and the one source for replacement adapters I
did find online sold me a part that had the same problem-shot springs (I
assume this, as I was getting the same chatter with the "new" part).  I
have Googled, searched on eBay, checked online used scientific/medical
equipment suppliers to no avail...but I will keep looking in case
something comes up.

So I guess my question is...would one of the newer cassette holders fit in
my microtomes?  In addition to the Reichert mentioned above, I also have
an American Optical 860.  Or is it possible to figure out how to replace
the springs in the thing? (I have half a mind to wander over to the
mechanical engineering dept. here to ask that question, but I figured I'd
start here on Histonet.) I would really hate to have to replace the
microtomes just because I can't get a cassette holder for them.

Thanks for all your help,

Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914

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[Histonet] Sectioning problem

[kgrobert]

I am working on an old microtome, a Reichert Histostat with a Tissue-Tek
II microtome chuck adapter, and the last time I had this machine cleaned
and rehabbed by my repairman, he said that the springs were shot on the
chuck adapter, resulting in the windowblind chatter that I was getting in
my sections. Unfortunately he did not have a replacement, Sakura doesn't
make or sell them anymore, and the one source for replacement adapters I
did find online sold me a part that had the same problem-shot springs (I
assume this, as I was getting the same chatter with the "new" part).  I
have Googled, searched on eBay, checked online used scientific/medical
equipment suppliers to no avail...but I will keep looking in case
something comes up.

So I guess my question is...would one of the newer cassette holders fit in
my microtomes?  In addition to the Reichert mentioned above, I also have
an American Optical 860.  Or is it possible to figure out how to replace
the springs in the thing? (I have half a mind to wander over to the
mechanical engineering dept. here to ask that question, but I figured I'd
start here on Histonet.) I would really hate to have to replace the
microtomes just because I can't get a cassette holder for them.

Thanks for all your help,

Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914

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Re: [Histonet] biohazard bags

[DKBoyd]

We were doing just that and our Infection Control Nurse has banded this 
practice.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







"Jeff Birkner"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
07/22/2010 03:11 PM

To

cc

Subject
[Histonet] biohazard bags






We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirk...@colabserv.com

 


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delete the
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RE: [Histonet] biohazard bags

[Stacy McLaughlin]

Re-use of specimen transport bio-bags is a big NO-NO in my laboratory.  You run 
the risk of accidentally using one that is contaminated. (This actually 
happened, and it was not a pretty sight.) 
You can't guarantee that the outside of that specimen container in the bag is 
not contaminated, even though it looks clean.  You don't know the practices of 
the person that collected it.  This could be a big liability and the possible 
consequences just aren't worth it.  
Just my 2 cents.
Stacy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Jeff
Birkner
Sent: Thursday, July 22, 2010 3:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] biohazard bags


We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirk...@colabserv.com

 


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only for the use of the recipient(s) named above, and may be legally privileged.
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RE: [Histonet] Biocare P63 with Dako Flex detection

[Cynthia Pyse]

Cathy
Have you tried adding an EnVision FLEX mouse linker to your protocol.
Sometimes that will work.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
cathy.crump...@tuality.org
Sent: Wednesday, July 21, 2010 3:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biocare P63 with Dako Flex detection


   Hi  all, I have ran into a troubleshooting dilemma and was wonderi=g
   if  anyone  else could help.  Is there anyone out there that uses the
Biocare  Medical  P63 concentrated antibody with the Dako TRS and Flex
   detec=tion  kit?  I am curious what dilution you use and if you have
   to  do  an=ything  special  for  the  P63 (extra long retrieval, DAB
   enhancer, etc). = am having problems getting a strong signal for the
   basal cells in prosta=e.  I am running it at 1:50 with 40 minutes in
   pH  9  retrieval  (which  =seems  too  long to me but does help).  A
   dilution with the same protoco= at 1:100 is way too light.

   Thanks,
<=DIV>
   Cathy Crumpton HT(ASCP), Histology Lead
   Tuality Community Hosp=tal
   Hillsboro, OR 97123
   (503)681-1292
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[Histonet] biohazard bags

[Jeff Birkner]

We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirk...@colabserv.com

 


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only for the use of the recipient(s) named above, and may be legally privileged.
If the reader of this message is not the intended recipient, you are hereby 
notified that any dissemination, distribution, or copying of this communication,
or any of its contents, is strictly prohibited.  If you have received this
communication in error, please return it to the sender immediately and delete 
the
original message and any copy of it from your computer system.  If you have any
questions concerning this message, please contact the sender. 
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RE: [Histonet] HPV testing

[McMahon, Loralee A]

Hi Dr. Cartun, 

We are running the HPV Genepoint test from Dako (in situ test).  Along with the 
EGFR IHC.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun 
[rcar...@harthosp.org]
Sent: Thursday, July 22, 2010 11:01 AM
To: Histonet
Subject: [Histonet] HPV testing

What are labs doing for HPV testing on head and neck cancers when a request is 
received from a clinician?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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[Histonet] Re: Histonet Digest, Vol 80, Issue 26

[Stephanie Rodriguez]

As do we.


Stephanie Rodriguez, HTL(ASCP), QIHC
Senior Molecular Technologist-FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA
(206) 374-9000 




On 7/22/10 10:04 AM, "histonet-requ...@lists.utsouthwestern.edu"
 wrote:

> 
> Message: 4
> Date: Thu, 22 Jul 2010 08:18:27 -0700
> From: "Tench, Bill" 
> Subject: [Histonet] hpv testing on head and neck ca's
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local>
> Content-Type: text/plain; charset=us-ascii
> 
> We do p16 on squamous ca of the head and neck region routinely
>  
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> bill.te...@pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604



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[Histonet] Histology metrics

[Scherer, Kris]

We are in need of a metrics for use in requesting an additional histotech.  
Does anyone have a system in place for presenting the need for an additional 
FTE, based on volume, worklow, vacations, etc?
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[Histonet] Laboratory Labeling Systems...

[Barone, Carol]

HistonettersWhat are your thoughts: 
 
We are getting ready to purchase a labeling system for our Core
Facility. We have limited $'s and have demo'ed three systems. We have
many different projects,with varying numbers of samples, blocks and
slides. We would like to cover the widest amount of needs, with the
systemnot just cassettes and slides, but samples in freezers to
antibodies we have alloquotted. We are about to purchase a two-D
bar-coding system that can label everything we need and work with our
present EXCEL data bases.  We are leaning toward a Brady System, where
we can purchase individually the parts we need for labeling cassettes to
eppy's and we especially like that they... not only have a label that
can be attached to frozen vials... but that there are several types of
scanners available for the different stations in our lab. This system
meets our CER requirements and is up-gradable for adding more stations
if needed at alater time. 
 
Sounds too good to be trueso that is where you all come in. 
 
Is anyone using a Brady Labeling System? Good? Bad? Problems? What are
your thoughts?  Thanks for the impromptu survey. We plan to order in
August, if there are no "big" issues, with those that use it. We demo's
the labels and are happy with them. We are going alpha numeric/2-D...3
lines of text, is needed for our research work. Thanks for your
thoughts. 
 
C. Barone
Director Histotechnology Core Lab
cbar...@nemours.org
 
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[Histonet] Surplus Equipment

[Gary P Rogers]

Good Morning Histonetters!

I would like to speak to anyone that might have any surplus Histology
equipment. I am specifically interested in the following items:

1.  Leica XL Autostainer

2. Sakura Tissue Processors-"E" Series or Newer

3. Sakura SCA Tape Coverslippers

4. Leica Microtomes

5. Leica Cryostats

Thanks in advance for your response

Gary Rogers

484-886-6870

g...@usedhistologyequipment.com

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[Histonet] hpv testing on head and neck ca's

[Tench, Bill]

We do p16 on squamous ca of the head and neck region routinely
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
bill.te...@pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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[Histonet] OmniTrax

[Victor Tobias]

 Good morning all,

Just to clarify Dr. Perkocha's request on tracking programs. OmniTrax 
was developed by the Pathology Dept. at University of Washington Medical 
Center. For more information please go to 
http://pathwaypathologyconsultants.com/


Victor

--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
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disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


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[Histonet] HPV testing

[Richard Cartun]

What are labs doing for HPV testing on head and neck cancers when a request is 
received from a clinician?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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RE: [Histonet] ER/PR ON BENCHMARK XT

[Sebree Linda A]

Adrienne,

ER:  Leica (Vision Biosystems), clone 6F11, mild CC1, 48" @ 42 degrees
C, AB block, Hem II / 8"

PR:  VMS, clone 1E2,  standard CC1, 32" @ 42 degrees C, AB block, Hem II
/ 8"


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Aperghis Kavanagh
Sent: Wednesday, July 21, 2010 2:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ER/PR ON BENCHMARK XT

Hi All,

Does anyone do ER/PR and/or CA15.3 on the Ventana Benchmark XT?  If so,
can you please tell me what protocol you are using?

Thank you in advance,


Adrienne Kavanagh
US PATH
30 W. Century Road
Suite 255
Paramus NJ 07652 

- Original Message -
From: histonet-requ...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, July 21, 2010 1:03:27 PM
Subject: Histonet Digest, Vol 80, Issue 24

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email,
send a message with subject or body 'help' to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."


Today's Topics:

1. Fluid for snap freezing (as in histobath fluid) (ebin...@mmm.com) 2.
EXPOSE (Perry, Margaret) 3. Utilization of consultation services (Cristi
stephenson) 4. RE: Utilization of consultation services (Feher, Stephen)
5. Cryostat Decontamination (Myers, Karyn) 6. Per Diem Histotech Needed
(Orange County, California) (Paula Lucas) 7. Certification question
(jcarpenter...@aol.com) 8. Certification question
(jcarpenter...@aol.com) 9. RE: Certification question (Geils, Karen
Brinker) 10. RE: Certification question (Geils, Karen Brinker) 11.
decontamination of cryostat (Tench, Bill) 12. RE: Certification
question (Hannen, Valerie) 13. RE: Certification question
(Hannen, Valerie) 14. Histology Externships for New Grads. A message
from Pam Barker at RELIA (Pam Barker) 15. RE: decontamination of
cryostat (Jesus Ellin) 16. Re: decontamination of cryostat
(anni...@gmail.com)


--

Message: 1
Date: Tue, 20 Jul 2010 12:09:02 -0500
From: ebin...@mmm.com
Subject: [Histonet] Fluid for snap freezing (as in histobath fluid)
To: Histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset="US-ASCII"

I noticed that our Novec 7100 has been discussed many times on Histonet
as an alternative to isopentane in Histobath and we've had a number of
laboratories who have ordered our fluid for this purpose. I'd like to
learn more about what works well, whether this is the best fluid we have
to offer, how to understand this market. Is it just because it's
nonflammable that it's of interest? Or what other characteristics are
important? My division is primarily involved in Electronics Markets, but
if this is a new use, I'd really like to help and hopefully find a way
to make the product more accessible and make sure it works.
I'm looking for up to 10 hospitals or labs who would be willing to
participate in a formal evaluation (I would supply fluid). I believe I
can share results on Histonet if it's of interest.

Erin Binder
(651) 733 3203 office
(612) 290 6343 mobile
Market Development Mgr
Electronic Markets Materials Division


--

Message: 2
Date: Tue, 20 Jul 2010 14:49:21 -0500
From: "Perry, Margaret" 
Subject: [Histonet] EXPOSE
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

Content-Type: text/plain; charset="us-ascii"

Has anyone tried the new polyvalent polymer detection "EXPOSE" from
AbCam? What did you think of it?
Margaret SDSU


--

Message: 3
Date: Tue, 20 Jul 2010 14:03:59 -0700 (PDT)
From: Cristi stephenson 
Subject: [Histonet] Utilization of consultation services
To: Histo Net 
Message-ID: <84474.37770...@web81203.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello Histoland,
I am currently researching the utilization of consultation services for
our lab.  We are a physician owned lab and therefore only have one
pathologist on site at a time.  Does anyone else track this type of
information?  Would you be willing to share the percentage of cases sent
out to cases read?  Any assistance is greatly appreciated.
Thanks, Cristi

--

Message: 4
Date: Wed, 21 Jul 2010 09:03:16 -0400
From: "Feher, Stephen" 
Subject: RE: [Histonet] Utilization of consultation services
To: "Cristi stephenson" , "Histo Net"

Message-ID:
<73a7ed895ee0c24d9267ed814911df1912b75...@exchange.cmc-nh.org>
Co

[Histonet] Re: test animal vs. plant

[Birgitta Stephenson]

Hello Tamara,

Thanks for your reply. In the lab we have worked with the carbohydrate
ideas, so travelling down the same path. With the grindstones we are
looking for some tests that we can also carry out in the field as
sometimes we have grinding hollows or stones in situ which can not be
moved. This is why we thought simple visual staining and utilising a
hand held digital microscope could be the answer. However the staining
is not as straight forward as we'd hoped. I am thinking that if the
Phloroglucinol is light sensitive then perhaps counterstaining with
Toluidine Blue can highlight other areas. Problem is the residues are
water lifted and drowning the slide with tap water (well drops of) means
that the residue at times almost vanishes. Any more thoughts are most
welcome.

Thnaks again

Birgitta Stephenson

Research Microscopy Lab, University of Queensland
On Tue, 20 Jul 2010 11:41:51 -0600, "Tamara A Howard" 
said:
> I saw your post on the Histonet - it seems that there must 
> specific tests that could do what you need. I Googled the 
> word string: "test distinguish plant animal" & got the 
> following promising hit:
> 
> http://www.hsc.csu.edu.au/chemistry/options/forensic/2964/ch992nov03.html
> 
> My guess is that there would be more info from forensic 
> science sites; they must have listservers, too.
> 
> It sounds like a very interesting project - I'm envious!
> 
> Tamara
> 
> ***
> Tamara Howard
> Cell Biology & Physiology
> UNM-HSC
> Albuquerque, NM
> ***
-- 
  Birgitta Stephenson
  bstep...@fastmail.fm

-- 
http://www.fastmail.fm - Same, same, but different...


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Re: [Histonet] Toluidine Blue and staining archaeological residues.

[Birgitta Stephenson]

Hello Paula,

Thanks for the reply. We do use IKI regualarly for starch staining,
however once the slide is stained it is difficult to counterstain to
detect animal residues which might be present. The residues from
grindstones have lost a lot of their microsopic diagnostic properties
and we are hponing that staining the residues will help differentiate
between intensive specialised seed grinders versus multi-purpose
expedient grinders. I am finding that the Phloroglucinol stain is light
sensitive and therefore not permanent which allows for some
counterstaining but still the technique is a work in progress. Any more
thoughts most welcome.

Regards

Birgitta Stephenson
Research Microscopy Lab, University of Queensland


On Tue, 20 Jul 2010 06:51:09 -0700 (PDT), "Paula Pierce"
 said:
> Starch (plants) + Iodine = Black solution.
> 
> 
> 
> 
> 
> From: Birgitta Stephenson 
> To: histonet@lists.utsouthwestern.edu
> Sent: Mon, July 19, 2010 8:19:27 PM
> Subject: [Histonet] Toluidine Blue and staining archaeological residues.
> 
> Hello Histonetters,
> 
> I am working with archaeological residues lifted from Holocene
> grindstones. I was trying to find a stain that could differentiate
> between plant and animal tissue in one hit. I have been using buffered
> Toluidine Blue solutions however given that these are ancient residues
> which are lifted using 20ul of water, the subtle colour differences
> between blues, purples, and violets have not been useful. I was looking
> at the possibility of counterstaining the Toluidine Blue stained
> residues with say Phloroglucinol or IKI which would highlight the plant
> component of the residue and then see if what was left looked like
> collagen.  Has anyone tried this type of counterstaining? OR does anyone
> know of a stain that would colour differently for plant and animals
> understanding that this is not a tissue section but microscopic residues
> in solution?
> 
> Thanks 
> Birgitta Stephenson
> The Research Microscopy Laboratory, University of Queensland.
> -- 
>   Birgitta Stephenson
>   bstep...@fastmail.fm
> 
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> http://www.fastmail.fm - Access your email from home and the web
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-- 
  Birgitta Stephenson
  bstep...@fastmail.fm

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