[Histonet] Sakura X50 Microwave Tissue Processor

[Carlos Hernandez]

Thanks to everyone who gave some input on the X50, it helped a lot and has me 
thinking twice about that particular piece of equipment.

Carlos


  
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[Histonet] Snap freezing using a "Dry Shipper?"

[Hugh Luk]

Hi Histonetters,


I need some advice as my group is tasked to procure tissue from surgery sites 
off-campus.  Logistics within those other hospitals
do not allow a liquid nitrogen source, and my health and safety folks have
asked us to consider using a "Dry Shippers" or cryoport for snap
freezing.  Does anyone do this?  


We are concerned that the vapor alone would be insufficient to preserve RNA,
DNA, and morphology.   


We currently use a dewar for liquid nitrogen preservation, but it does not 
involve the whole 'transport issue'. 



Research or hospital perspectives are welcome.



Thanks in advance and have a good week.


Hugh 

Cancer research center of Hawaii



  
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[Histonet] Form for validating slides on digital slide scanner

[Mighnon Lashus]

We are just getting started with our Digital slide scanner and I am looking for 
help.  Anything you would be willing to share in terms of SOPs, Policies in 
regards to CAP and validation forms used when you are initially validation the 
machine would be helpful.
Thanks,

Mighnon Lashus, HT (ASCP)
PathGroup Lab
4071 S. Access Road, Suite 107
Chattanooga, TN  37406
423-493-0207
423-493-0208 fax
mlas...@pathgroup.com




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[Histonet] Re: Solvent Recycler

[Miyamoto, Garret T Mr CIV USA USAMEDCOM]

Timothy,
We have the B/R 9700 ProCycler to recycle alcohol, xylene, and formalin.  The 
Advantage must be a newer model.  About 70 gal of alcohol, 40 gal of xylene, 
and 20 gal of formalin is recycled every 3 months.  This recycler is easy to 
use and works well for us.  As with any equipment, you need to maintain it by 
doing pm checks (replacing parts) as needed.

Garret

- Original Message -
From: histonet-requ...@lists.utsouthwestern.edu
Date: Saturday, September 11, 2010 7:09 am
Subject: Histonet Digest, Vol 82, Issue 13
To: histonet@lists.utsouthwestern.edu


> Send Histonet mailing list submissions to
>   histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>   http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
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> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Re: H&E+ Alcian Blue (kim.dona...@bhcpns.org)
>   2. solvent recycling (Timothy Chilton)
>   3. Liver Processing Question (Herrick, James L. (Jim))
>   4. RE: solvent recycling (WILLIAM DESALVO)
>   5. RE: RE: Ventana vs. Leica (Makhijani, Nalini S)
>   6. RE: LIS, HIS question (Sharon Scalise)
>   7. Microm HM330 (Yu, Jian)
>   8. RE: LIS, HIS question (Feher, Stephen)
>   9. RE: solvent recycling (Feher, Stephen)
> 
> 
> ---
> ---
> 
> Message: 1
> Date: Fri, 10 Sep 2010 12:46:11 -0500
> From: kim.dona...@bhcpns.org
> Subject: Re: [Histonet] H&E+ Alcian Blue
> To: Deborah Faichney <
> Cc: "histonet@lists.utsouthwestern.edu"
>   <,
>   histonet-boun...@lists.utsouthwestern.edu
> Message-ID:
>   <
> Content-Type: text/plain; charset="US-ASCII"
> 
> It wont remain turquoise as long as you use the eosin. At least this is my 
> experience. I have known some Pathologist to read the mucin that way 
> (purple). 
> 
> 
> 
> 
> Kim Donadio 
> Pathology Supervisor
> Baptist Hospital
> 1000 W Moreno St.
> Pensacola FL 32501
> Phone (850) 469-7718
> Fax (850) 434-4996
> 
> 
> 
> Deborah Faichney < 
> Sent by: histonet-boun...@lists.utsouthwestern.edu
> 09/09/2010 03:44 AM
> 
> To
> "histonet@lists.utsouthwestern.edu" <
> cc
> 
> Subject
> [Histonet] H&E+ Alcian Blue
> 
> 
> 
> 
> 
> 
> Hello all,
> 
> I have a request to carry out a combined staining with H&E + Alcian Blue 
> pH2.5.I have tried in vain to get this to work but regardless of the 
> order of staining the end result is dark blue/purple mucin.  I have 
> carried out a parallel experiment whereby the staining has been checked 
> microscopically then stopped after each of the dyes.   (Thus giving 3 
> slides stained with:  AB, AB+H and AB+H+E)  The AB and AB+H are really 
> nicely stained but as soon as the eosin is added (using 2 different stains 
> and a variety of times) the mucin staining looks similar to the nuclear 
> stain.  I am expecting the alcian blue to remain turquoise: should it?
> 
> For information, I am trying to stain salmon intestine at 5 microns for 
> using the following:
> 
> Alcian Blue 8GX (certified), pH has been checked
> Haematoxylin Z (Cellpath Uk)
> 1% aq Eosin (Cellpath uk) and lab prepared solution from dye.
> 
> Thanks from a frustrated technician!
> 
> Debbie Faichney
> Histopathology
> Institute of Aquaculture
> University of Stirling
> Scotland
> UK
> 
> 
> 
> 
> 
> 
> 
> 
> -- 
> The Sunday Times Scottish University of the Year 2009/2010
> The University of Stirling is a charity registered in Scotland, 
> number SC 011159.
> 
> ___
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> 
> 
> 
> -
> All electronic data transmissions originating from or sent to
> Baptist Health Care Corporation (BHC) are subject to monitoring.
> This message along with any attached data, are the confidential and
> proprietary communications of BHC and are intended to be received
> only by the individual or individuals to whom the message has been
> addressed. If the reader of this message is not the intended
> recipient, please take notice that any use, copying, printing,
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> strictly prohibited and may violate State or Federal Law. If you
> have received this transmission in error, please delete or destroy
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> 
> Message: 2
> Date: Fri, 10 Sep 2010 13:13:13 -0500
> From: "Timothy Chilton" <
> Subject: [Histonet] solvent recycling
> To: <
> Message-ID: <
> Content-Type: tex

Re: [Histonet] Napsin A

[Mark Tarango]

Hi Jessica,

Our control for this antibody is normal lung, adenocarcinoma of the lung,
and thyroid.  We use this same block for other stains too (TTF-1,
sufactant-A, EMA, etc.) but if I were going to make a control block specific
for this antibody, I would want to include a squamous cell carcinoma of the
lung to show that it's negative for napsin-A.  You may see a very tiny
amount of staining on the squamous cell carcinomas where the
pulmonary-aveolar macrophages are eating up the keratin, but the tumor cells
will be negative.

Ovarian carcinomas can also stain.  I would include a few of those too so
the docs can see what that looks like.
I would also make sure to include thyroid and thyroid carcinomas.  With a
particular vendor's antibody, napsin-a seems to stain thyroid (I don't want
to name names), but with Novocastra's antibody it doesn't.  It was helpful
to our pathologists to have an antibody that doesn't stain thyroid.

Thanks
Mark

On Mon, Sep 13, 2010 at 8:57 AM, Jessica Piche  wrote:

> Hello Everyone,
>
> We are in the process of working up Napsin A. I was wondering what tissue
> others are using for multi tissue blocks to validate the anitbody? Thanks,
>
>
> Jessica Piche-Grocki, HT(ASCP)
> Waterbury Hospital, CT
>
>
>
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[Histonet] coverslip removal

[Perry, Margaret]

I  am using aqueous mounting media, faramount,  for the first time.  The slides 
I coverslipped several days ago look dry.  Will soaking in water remove the 
coverslip?

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

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Re: [Histonet] Napsin A

[Jessica Piche]

Hi Loralee,
Thanks for your response. What did you use for the multi normal tissue block? 
Kidney?
Also for the multi-tumor block did you just use tumors from organs that  you 
knew were negative for Napsin or did you use both positive and negative tumors? 

Thanks, 
Jessica





From: "McMahon, Loralee A" 
To: Jessica Piche ; "histonet@lists.utsouthwestern.edu" 

Sent: Mon, September 13, 2010 1:29:58 PM
Subject: RE: [Histonet] Napsin A

We are using a Lung Adenocarcinoma as a daily control.  Although I have noticed 
that normal lung macrophages will pick it up. 

We ran our validation of the antibody using a multi tumor block (contains 
various tumors from various organs) and a multi normal tissue block to ensure 
that it stained only the expected things and not anything else.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche 
[jhist...@yahoo.com]
Sent: Monday, September 13, 2010 11:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Napsin A

Hello Everyone,

We are in the process of working up Napsin A. I was wondering what tissue
others are using for multi tissue blocks to validate the anitbody? Thanks,


Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital, CT



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RE: [Histonet] new lab design

[jstaruk]

We recently built our new facility from the ground up.  Having worked in
various histology labs, I knew what works and what doesn't work.  Planning
the lab around work-flow is crucial.  I started the design by simply writing
the name of each room I wanted constructed on a sticky-note.  These were
initially all the same size.  Then I placed each room where I wanted it so
work would flow from room to room.  Later, I adjusted the sizes to fit the
square footage of the outside walls.  I even planned on a few through the
wall pass-throughs that I knew would help in work-flow.  As the plans began
to develop, I added where I wanted electrical plugs, lights, sinks, etc.
When the architect drew up the official plans, I was still able to adjust
some walls until everything was perfect.  It took about 3 months to plan the
lab and it's perfect for our work load.

Jim

___
James E. Staruk HT(ASCP)
 www.masshistology.com
   www.nehorselabs.com
 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, September 13, 2010 8:10 AM
To: 'Histonet'
Subject: [Histonet] new lab design

Good morning all.  I am in the process of designing a new lab.  We have
grown beyond our walls and will be moving to a new building.  If anyone
has any great suggestions or ideas they would like to share I'd love
your input!
I'm still looking for a couple of tech too!
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists,
Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email:
lbla...@digestivespecialists.com

FW: [Histonet] Liver Processing Question

[Herrick, James L. (Jim)]

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herrick,
James L. (Jim)
Sent: Friday, September 10, 2010 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Liver Processing Question

Hello everyone,

I am trying to process and embed liver tissue in MMA. I used the same
protocol that has worked very well a hundred times over, for harder
tissues, but when used on the liver tissue, polymerized into a
relatively soft and rubbery textured plastic for one of the two
specimens (the second of the two also being too soft to properly
section). If anyone would have any ideas/suggestions or protocols for me
to follow, it would be very much appreciated. Thanks and have a great
day!!

Jim


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RE: [Histonet] Napsin A

[McMahon, Loralee A]

We are using a Lung Adenocarcinoma as a daily control.  Although I have noticed 
that normal lung macrophages will pick it up. 
We ran our validation of the antibody using a multi tumor block (contains 
various tumors from various organs) and a multi normal tissue block to ensure 
that it stained only the expected things and not anything else.  

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche 
[jhist...@yahoo.com]
Sent: Monday, September 13, 2010 11:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Napsin A

Hello Everyone,

We are in the process of working up Napsin A. I was wondering what tissue
others are using for multi tissue blocks to validate the anitbody? Thanks,


Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital, CT



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RE: [Histonet] new lab design

[Morken, Tim]

Steve's workshop at NSH last year was a good intro to lab design. The most 
significant take-away was that they did many, many modifications on paper 
before they ever did anything for real...and all with LEAN principle driving 
the design. 


Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen
Sent: Monday, September 13, 2010 9:29 AM
To: Blazek, Linda; Histonet
Subject: RE: [Histonet] new lab design

Hi Linda,

We designed one from scratch without having a previous Path Lab in the
hospital before.  We are doing a workshop to that end at NSH in Seattle
(WS 50).  If you cannot attend the workshop, I will be happy to help in
any that I can. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, September 13, 2010 8:10 AM
To: 'Histonet'
Subject: [Histonet] new lab design

Good morning all.  I am in the process of designing a new lab.  We have
grown beyond our walls and will be moving to a new building.  If anyone
has any great suggestions or ideas they would like to share I'd love
your input!
I'm still looking for a couple of tech too!
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists,
Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email:
lbla...@digestivespecialists.com

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RE: [Histonet] new lab design

[Podawiltz, Thomas]

FYI, 

I have been to Steve's lab. They have a great layout. A lot of time and effort 
was spent in the design of it and it shows. 


Tom Podawiltz HT (ASCP) 
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen
Sent: Monday, September 13, 2010 12:29 PM
To: Blazek, Linda; Histonet
Subject: RE: [Histonet] new lab design

Hi Linda,

We designed one from scratch without having a previous Path Lab in the
hospital before.  We are doing a workshop to that end at NSH in Seattle
(WS 50).  If you cannot attend the workshop, I will be happy to help in
any that I can. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, September 13, 2010 8:10 AM
To: 'Histonet'
Subject: [Histonet] new lab design

Good morning all.  I am in the process of designing a new lab.  We have
grown beyond our walls and will be moving to a new building.  If anyone
has any great suggestions or ideas they would like to share I'd love
your input!
I'm still looking for a couple of tech too!
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists,
Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email:
lbla...@digestivespecialists.com

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THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential 
information intended only for the use of the recipient(s) named above. If you 
are not the intended recipient, you may not print,distribute, or copy this 
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please notify the sender by return e-mail and delete this message and any 
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RE: [Histonet] new lab design

[Feher, Stephen]

Thanks Tim! 


Steve

-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org] 
Sent: Monday, September 13, 2010 12:38 PM
To: Feher, Stephen; Blazek, Linda; Histonet
Subject: RE: [Histonet] new lab design

FYI, 

I have been to Steve's lab. They have a great layout. A lot of time and
effort was spent in the design of it and it shows. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer LRGHealthcare
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
Sent: Monday, September 13, 2010 12:29 PM
To: Blazek, Linda; Histonet
Subject: RE: [Histonet] new lab design

Hi Linda,

We designed one from scratch without having a previous Path Lab in the
hospital before.  We are doing a workshop to that end at NSH in Seattle
(WS 50).  If you cannot attend the workshop, I will be happy to help in
any that I can. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, September 13, 2010 8:10 AM
To: 'Histonet'
Subject: [Histonet] new lab design

Good morning all.  I am in the process of designing a new lab.  We have
grown beyond our walls and will be moving to a new building.  If anyone
has any great suggestions or ideas they would like to share I'd love
your input!
I'm still looking for a couple of tech too!
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists,
Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email:
lbla...@digestivespecialists.com

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THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential
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If you are not the intended recipient, you may not print,distribute, or
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delete this message and any attachments from your computer. Any views or
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RE: [Histonet] new lab design

[Feher, Stephen]

Hi Linda,

We designed one from scratch without having a previous Path Lab in the
hospital before.  We are doing a workshop to that end at NSH in Seattle
(WS 50).  If you cannot attend the workshop, I will be happy to help in
any that I can. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Monday, September 13, 2010 8:10 AM
To: 'Histonet'
Subject: [Histonet] new lab design

Good morning all.  I am in the process of designing a new lab.  We have
grown beyond our walls and will be moving to a new building.  If anyone
has any great suggestions or ideas they would like to share I'd love
your input!
I'm still looking for a couple of tech too!
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists,
Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email:
lbla...@digestivespecialists.com

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RE: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded)

[gayle callis]

YiJing, 

You could be under- processing the mouse paws.  We extend processing time
for mouse paws during dehydration, clearing and especially infiltration with
paraffin and done with vacuum. Poor paraffin infiltration can really create
shredding problems. There is a simple, cheap way to check endpoint of
decalcification when decalcifying bones.  It is called weight loss/weight
gain method and works for both EDTA and any acid decalcification protocols.
Endpoint determination is important to know when calcium is totally removed
so processing and microtomy problems do not occur.  Bending or "feeling
soft" generally is a poor way to determine total calcium removal as small
calcium deposits are not detectable this way.  X-ray is the most sensitive,
but not everyone has the technology/equipment for that. Chemical endpoint
testing is also simple and easy to do. I doubt the blades are the problem,
but more likely decalcification and processing. I would be happy to send the
weight loss/weight gain and chemical test methods to you.  

Using a harder paraffin helps with bone, Tissue Prep 2 (Thermo Scientific
under Fisher Scientific label) is one of several available although other
paraffins should work if infiltration is done properly. 

You did not provide a processing schedule for these decalcified mouse paws.
Is this done manually or on an automate processor?  Times in each solution?
Time of paraffin infiltration? 

Gayle M. Callis
HTL/HT/MT(ASCP) 
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kristen
Lauing
Sent: Monday, September 13, 2010 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Trouble shooting decalcified bone sections (paraffin
embedded)

I section EDTA-decalcified mouse tibias frequently, and I find it helps if
the paraffin is very very cold during sectioning - if the tissue starts
shredding again, I press a large piece of ice to the block for a minute to
cool it down without having to remove the block from the microtome.  It
seems to work well for me, although it may not be the most technically sound
way to get the job done.  I can usually get a good ribbon of sections this
way when I'm having difficulty with a particular sample.
I have to use an old microtome and blades because I'm a student at a
research institution, without the help of professional histotechs.  Our
tibias are decalcified in 10% EDTA for 7 days at 4 degrees with agitation
with frequent solution changes.

Kristen

>>> "CHEN, YIJING"  09/12/10 12:50 AM >>>
Hi,
We are having difficulty sectioning paraffin-embedded decalcified adult
mouse autopods (paws).  The tissues shatter as soon as they hit the blade
when sectioned.  

The autopods were soaked in CalEX for 4 days at room temp and felt extremely
soft before embedding, suggesting effective decalcification.  We use the
Sturkey EXTREMUS low profile disposable knives on our microtome.  These
knives are said to be coated with the hardest nitride coating available.
Any suggestions will be greatly appreciated.
Sincerely,
Yijing


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[Histonet] Napsin A

[Jessica Piche]

Hello Everyone,

We are in the process of working up Napsin A. I was wondering what tissue 
others are using for multi tissue blocks to validate the anitbody? Thanks, 


Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital, CT



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Re: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded)

[Kristen Lauing]

I section EDTA-decalcified mouse tibias frequently, and I find it helps if the 
paraffin is very very cold during sectioning - if the tissue starts shredding 
again, I press a large piece of ice to the block for a minute to cool it down 
without having to remove the block from the microtome.  It seems to work well 
for me, although it may not be the most technically sound way to get the job 
done.  I can usually get a good ribbon of sections this way when I'm having 
difficulty with a particular sample.
I have to use an old microtome and blades because I'm a student at a research 
institution, without the help of professional histotechs.  Our tibias are 
decalcified in 10% EDTA for 7 days at 4 degrees with agitation with frequent 
solution changes.

Kristen

>>> "CHEN, YIJING"  09/12/10 12:50 AM >>>
Hi,
We are having difficulty sectioning paraffin-embedded decalcified adult
mouse autopods (paws).  The tissues shatter as soon as they hit the blade
when sectioned.  

The autopods were soaked in CalEX for 4 days at room temp and felt extremely
soft before embedding, suggesting effective decalcification.  We use the
Sturkey EXTREMUS low profile disposable knives on our microtome.  These
knives are said to be coated with the hardest nitride coating available.
Any suggestions will be greatly appreciated.
Sincerely,
Yijing


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[Histonet] SOX-10

[Angela Bitting]

Happy Monday Histonetters,
 
I'm wondering if anyone can share a protocol for running SOX-10 on the Ventana 
BenchmarkXT?
(On FFPE tissue)
 
Thanks,
Angie


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RE: [Histonet] Flat Ice???

[Kim . Donadio]

Thanks. I am going to try that "ice rink: tip. :-) 

What I do to keep my blocks from sliding all over the place now is put a 
kimwipe on my wet ice. They can cool and I also like to hydrate my blocks 
a little. I feel it gives me a better looking section histologically. my 2 
cents as well. ;o)



Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



 
Sent by: histonet-boun...@lists.utsouthwestern.edu
09/11/2010 09:22 PM

To
"'WILLIAM DESALVO'" , , 
, "'histonet'" 
cc

Subject
RE: [Histonet] Flat Ice???






I much prefer the "wet ice" approach to the more dry variety of the cool
trays.  The "wet" ice gets my blocks colder and I like the fact that it
introduces a small amount of moisture into the already faced block.  My
blocks are not on the ice for long periods of time, so over-saturating 
them
with water is not an issue.

To keep my trays from growing mountains, I have found that if I fill them 
up
3/4 of the way and let them freeze and then follow up with the other 1/4
later in the day, I get nice, flat little "ice rinks" that I can lay my
blocks on.  It's not time consuming either, just have to remember to go 
back
and pour more water on them!  By the way, this works on ice cube trays as
well as steel pans we have.  I don't really care for the pans, but some of
my techs do.  They take a little more work to make them ice rinks.  ;o)

My $0.02.  :o)

Michelle


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Friday, September 10, 2010 12:03 PM
To: msherw...@partners.org; sraib...@yahoo.com; histonet
Subject: RE: [Histonet] Flat Ice???



For quality assurance and safety concerns, I would suggest moving away 
from
the "wet ice" and go with a sealed freezing tray. We moved to the small
Histo-Cool ice trays w/ tray. 
 
 http://www.labstore.com/catalog/index.cfm?Category_ID=375

William DeSalvo, B.S., HTL(ASCP)




 
> Date: Fri, 10 Sep 2010 10:58:01 -0400
> From: msherw...@partners.org
> To: sraib...@yahoo.com; Histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Flat Ice???
> CC:
> 
> We use a styrofoam container and have no problem with it freezing 
> flat. Are you containers sitting flat in the freezer?
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan 
> Raibley
> Sent: Friday, September 10, 2010 10:52 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Flat Ice???
> 
> Hello! I would greatly appreciate any tips or tricks to getting ice to 
> freeze flat in the pan! We are tired of opening the freezer to see 
> mountains that have formed in our pans overnight, making it hard to 
> try and fit all the blocks we need to microtome on them. We have two 
> freezers and they both have this problem. Help please! Thanks!
> 
> 
> Susan Bincsik, HT (ASCP)
> 
> 
> 
> 
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[Histonet] re: new lab design

[Paul Verden]

Congratulations on your move to a new building and the opportunity to
have a "fresh" start in a new lab.  

 

The first thing I would suggest is to NOT blindly depend on a laboratory
design firm to design your lab.  Consider the design and operation of
your new lab like you would buying a new car.  Would you buy a new car
from a dealer just because they tell you that they know what they are
doing and they know what you need?  Or, would you prefer to a car built
for you that has all the elements you need to function in the way you
want it to function?  

 

Second, make sure you are involved in all the design aspects of your
laboratory.  Don't trust anyone but yourself and your techs to know
exactly what you need.

 

Third, and most important, be sure to involve your lab/cyto/histo
technologists in the design.  No one knows better about how they would
like their work area to be designed than the people doing the actual
work.  You will probably get some of your best ideas from the troops.
And, they will greatly appreciate the opportunity to input on the design
and have a much greater "buy-in" when all is said and done.

 

Good luck on your endeavor.  You will get great advice from your peers
on the histonet.

 

Paul

Supervisor - Cytology and molecular

UroPartners, LLC

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[Histonet] Histotechs Needed!!

[Matthew Ward]

Good Morning, 

 

Our team here at Personify has recently been partnered with a World Leader
in Cancer Diagnostics who is going through a large expansion due to their
recent and continued success in the Immunohistochemistry market. Due to this
expansion we are currently seeking Histotechs who have experience working
with IHC analyzers that would be interested in Field Support roles. 

 

 

 

These Positions Offer:

 

 

 

- Outstanding Base Salary and Bonus!

 

- Gold Standard Benefits including but not limited to Medical, Cell Phone,
Laptop, Car Allowance, Expenses, 401k, Paid Vacation!

 

- Opportunity for Career Advancement!

 

 

 

We currently have positions open in the following areas and will have more
locations throughout the year!

 

 

 

Southeast Florida (Miami/ FT. Lauderdale)

 

Boston/ New England

 

MD/VA/DC

 

NYC/Bronx/NJ

 

TN/MS/AR

 

 

 

If you are interested in learning more please contact me directly at

800.875.6188 ext. 103 or m...@personifysearch.com

 

 

Matt Ward

Account Executive

Personify

201 Shannon Oaks Circle, Suite 101

Cary, North Carolina 27511

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

   www.personifysearch.com

 

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[Histonet] new lab design

[Blazek, Linda]

Good morning all.  I am in the process of designing a new lab.  We have grown 
beyond our walls and will be moving to a new building.  If anyone has any great 
suggestions or ideas they would like to share I'd love your input!
I'm still looking for a couple of tech too!
Thanks,
Linda


Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com

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