Re: [Histonet] IHC FILD

[Jennifer MacDonald]

http://www.ascp.org/FunctionalNavigation/certification/QualificationinImmunohistochemistryQIHC.aspx

Here is the information from the ASCP website.





Andrea  
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/15/2010 05:56 PM

To
"Langenberg, Stacey" 
cc
Debora Probst , 
"histonet@lists.utsouthwestern.edu" , 
"histonet-boun...@lists.utsouthwestern.edu" 

Subject
Re: [Histonet] IHC FILD






Hi, how do you go about taking this test. are there prerequisite courses 
to take?



On 2010-10-15, at 3:25 PM, "Langenberg, Stacey" 
 wrote:

> Well said Mark!
> Sent via BlackBerry from T-Mobile
> 
> -Original Message-
> From: Mark Turner 
> Sender: "histonet-boun...@lists.utsouthwestern.edu"
>
> Date: Fri, 15 Oct 2010 12:46:59 
> To: Debora Probst; 
histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] IHC FILD
> 
> Since it wasn't a requirement for my position, I took the exam for my 
own personal satisfaction.  I highly recommend taking it if you have the 
chance and not worrying about whether you will receive extra money. 
Successfully passing the exam is a personal milestone and may not be 
recognized by your current employer, however it just might make a future 
one consider investing a little stronger in you because you have invested 
in yourself.
> 
> Good luck!
> 
> Mark Turner
> 
> 
>  Debora Probst  wrote: 
>> Can anyone tell me if once you have taken the IHC certification test 
and
>> passed dose the administration consider that a specialty field and give
>> you a pay increase? Or is it just for a persons own gratification to
>> take it and pass? 
>> 
>> ___
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>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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Re: [Histonet] IHC FILD

[Andrea]

Hi, how do you go about taking this test. are there prerequisite courses to 
take?



On 2010-10-15, at 3:25 PM, "Langenberg, Stacey" 
 wrote:

> Well said Mark!
> Sent via BlackBerry from T-Mobile
> 
> -Original Message-
> From: Mark Turner 
> Sender: "histonet-boun...@lists.utsouthwestern.edu"
>   
> Date: Fri, 15 Oct 2010 12:46:59 
> To: Debora Probst; 
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] IHC FILD
> 
> Since it wasn't a requirement for my position, I took the exam for my own 
> personal satisfaction.  I highly recommend taking it if you have the chance 
> and not worrying about whether you will receive extra money.  Successfully 
> passing the exam is a personal milestone and may not be recognized by your 
> current employer, however it just might make a future one consider investing 
> a little stronger in you because you have invested in yourself.
> 
> Good luck!
> 
> Mark Turner
> 
> 
>  Debora Probst  wrote: 
>> Can anyone tell me if once you have taken the IHC certification test and
>> passed dose the administration consider that a specialty field and give
>> you a pay increase? Or is it just for a persons own gratification to
>> take it and pass? 
>> 
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ___
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RE: [Histonet] IHC FILD

[O'Donnell, Bill]

I did not receive a pay increas. Personal gratification and a sense of
accomplishment. It was not a walk in the park by any stretch of the
imagination. I learned a great deal from taking it. - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
bsulli...@shorememorial.org
Sent: Friday, October 15, 2010 12:56 PM
To: Debora Probst
Cc: histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC FILD

I have never received any additional pay for any of my certifications.
It is something I did for myself.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor
Shore Memorial Hospital 609-653-3590


 

 "Debora Probst"

 
To 
 Sent by:
 
 histonet-bounces@
cc 
 lists.utsouthwest

 ern.edu
Subject 
   [Histonet] IHC FILD

 

 10/15/2010 01:53

 PM

 

 

 





Can anyone tell me if once you have taken the IHC certification test and
passed dose the administration consider that a specialty field and give
you a pay increase? Or is it just for a persons own gratification to
take it and pass?

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Re: [Histonet] negative controls

[Jan Shivers]

Linda,

My system of negative controls is identical to yours.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

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- Original Message - 
From: "Sebree Linda A" 

To: ; "Rene J Buesa" 
Cc: "Histo Net list server" 
Sent: Friday, October 15, 2010 11:07 AM
Subject: RE: [Histonet] negative controls


To clarify even further:  we cut 2 patient sections, put one on a slide with 
a section of positive control already on it.  This slide gets stained with 
the antibody  The other section goes on another slide and is run as a 
corresponding negative control using the same antibody protocol but 
substituting a negative control serum for the antibody, thus this is a 
"negative reagent control" slide.  Elements within the patient slide that 
received antibody and are expected to be negative, serve as a "negative 
tissue control".  Again, we run 1 negative control slide for EVERY 1 patient 
block in a run but only 1 negative control per any number of antibodies run 
on that same block, using the harshest protocol.  Only autopsy cases differ 
in that we run 1 negative control per TISSUE TYPE.



Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com]
Sent: Friday, October 15, 2010 10:48 AM
To: Rene J Buesa
Cc: Histo Net list server; Sebree Linda A
Subject: RE: [Histonet] negative controls

So for every HP you do, you process a control cassette with the patient
tissue cassette?  That seems like alot?  How do you get that many
control tissues on a daily basis?  What do you do with the remaining
tissue in the control block?  If you throw them away everyday, I would
be interested in some of them.  How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time?  We have always just had a bunch of
blocks that you cut a control from?  I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue.  If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times.  Why couldn't you
just have one for all 3 cases?  Then the next day have a fresh ONE for
that day, date them, and file them.  So if you needed to see the HP
control for October 15th, you could go pull the control for that day...

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




 Original Message 
Subject: RE: [Histonet] negative controls
From: Rene J Buesa 
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A , sgoe...@xbiotech.com
Cc: Histo Net list server 

Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.

--- On Fri, 10/15/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" 
Cc: "Histo Net list server" 
Date: Friday, October 15, 2010, 11:17 AM


  Why do you need a negative control for each block if you are runn=
ing
  the  same  antibody  on each patient block?  Is it just for case by c
 ase  reference  so  the negative is filed with the patient slide?  Why
  co=  uldn't  you  have  a control slide bank that was dated so all
the
  slides you d= id on that day, on that run, could be referenced back
to
  that control? = ; Just curious?

  Sarah Goebel, B.A., HT (ASCP)

  Histotechnician<= br>

  XBiotech USA Inc.

  8201 East Riverside Dr. Bld= g 4 Suite 100

  Austin, Texas  78744

  =

  (512)386-= 5107

   Original Message 
  Subject: RE: [Histonet] negative controls
  From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
  Date: Fri, October 15, 2010 8:08 am
  To:  "Victoria  Baker"  <[2]bakevict= o...@gmail

Re: [Histonet] IHC FILD

[Langenberg, Stacey]

Well said Mark!
Sent via BlackBerry from T-Mobile

-Original Message-
From: Mark Turner 
Sender: "histonet-boun...@lists.utsouthwestern.edu"

Date: Fri, 15 Oct 2010 12:46:59 
To: Debora Probst; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC FILD

Since it wasn't a requirement for my position, I took the exam for my own 
personal satisfaction.  I highly recommend taking it if you have the chance and 
not worrying about whether you will receive extra money.  Successfully passing 
the exam is a personal milestone and may not be recognized by your current 
employer, however it just might make a future one consider investing a little 
stronger in you because you have invested in yourself.

Good luck!

Mark Turner


 Debora Probst  wrote: 
> Can anyone tell me if once you have taken the IHC certification test and
> passed dose the administration consider that a specialty field and give
> you a pay increase? Or is it just for a persons own gratification to
> take it and pass? 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] Histotech needed in awesome Pensacola Florida

[Kim . Donadio]

Just a reminder for the weekend for all the Histotechs who are seeking a 
great position in a great place to live. I have a position coming 
available Very soon. 

I'm not a recruiter, just a supervisor who works the bench with you daily 
looking for someone smart and fun :-)

Have a great weekend everyone! 





Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

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[Histonet] High Paying Jobs in Laboratory

[Alisha Dynan]

 


 



Dear Beatrice,





 


I hope you are doing well. I am a recruiter at a highly successful and well 
respected healthcare recruiting firm.  I help place Lab Professionals in 
permanent positions across the country and I wanted to see if you or someone 
you know may be interested in exploring other career opportunities?  We are 
completely free of charge to job seekers and and we work on quite a few 
laboratory openings across the country. Our clients typically assist with 
relocation expenses. 




 

I am currently working with a private non-profit acute care hospital in 
Vermont. My client is looking to hire a full-time Medical Technologist for 
their 3rd shift. The shift is composed of three 12.5 hour shifts. The ideal 
candidate must be a certified Medical Technologist by the ASCP or equivalent 
and have 2+ years experience as a generalist. This is a fantastic opportunity 
at an outstanding hospital with a great reputation in this community. My 
clients offers some of the best benefits and compensation package around, 
including childcare reimbursement, commuter assistance, a retirement plan with 
a generous match, relocation assistance and tuition reimbursement. 
Additionally, the night shift comes with a generous shift differential. If you 
are interested in learning more about this position, please call or email me.

 

Below is a list of some of the other great opportunities we are currently 
working on. If you do not see an opening in a location in which you live or 
would like to live, please send me an email me a copy of your resume and let me 
know where you would be interested in a job. I will then tailor a search for 
you that is completely confidential and free to candidates.

 

Current Laboratory Opportunities (I just updated this list TODAY!!!): 

 


Medical Technologist/ Clinical Lab Scientist:


* NY - Rochester - Medical Technologist (3rd shift)


* NY - North of Albany - Medical Technologist (2nd shift)


* NY - Lake Placid - Medical Technologist (2nd or 3rd shift)


* NY - West of Albany - Medical Technologist (All Shifts)


* NY - Syracuse - Medical Technologist (2nd or 3rd shift)


* NY - NYC - Lead Medical Technologist (2nd shift)


* NY - Long Island - Medical Technologist (1st shift)


* PA - Lehigh Valley - Medical Technologist (2nd shift)


* CT - Medical Technologist (3rd shift)


* CT - MLT (1st shift)


* VT - Medical Technologist (3rd shift with 12 hours shifts)


* GA - Hematology Medical Technologist (1st shift)


* FL - Treasure Coast - Medical Technologist (3rd shift)


* OR - Coastal - Medical Technologist (3rd shift)


* OR - Medical Technologist (all shifts)


* WA - Medical Technologist


* AK - Medical Technologist


* NM - Medical Technologist (1st shift)


* CA - Los Angeles - Medical Technologist (all shifts)


* CA - Central Valley (rural) - Medical Technologist (3rd shift)


* CA - Sacramento - Laboratory Specialist



 



Lab Supervisor/Management Opportunities:


* MS - 2nd/3rd shift Laboratory Supervisor



* CT - Hematology Supervisor



* TX - Director of Laboratory Operations for Hospital System



* KY - Laboratory Supervisor



* CA - Los Angeles - Laboratory Supervisor



* TX - San Antonio - Laboratory Supervisor



* GA - HLA Supervisor



* PA - Director of Laboratory Operations



* NJ - General Manager of Anatomic Pathology



* WA - Quality Control Supervisor







 


Blood Bank (Bench and Management)








* WA - Transfusion Services Supervisor


* WA - Blood Bank Specialist


* WA - Blood Bank Educator


* IA - Blood Bank Technologist (2nd shift)


* NY - NYC - Immunohematologist (4pm12am shift)


* CA - San Fran - Specialist in Blood Bank


* MN - Component Laboratory Manager/ Blood Bank Supervisor


* NY - Syracuse - Blood Bank Specialist


* VA - Blood Bank Supervisor



 

Microbiology (Bench and Management)






* OR - Microbiology Technologist


* NY - Microbiology Lead Technologist


* MS - Microbiology Supervisor


* OR - Manager of Microbiology and Molecular Diagnostics


* CA - Los Angeles - Mycobacteriology Technologist


* NY - Microbiology Manager


* OH - Cinci - Microbiology Technologist (1st shift)































 











Cytotech and Histotech (Bench and Management)


* NY - NYC - Cytology Supervisor


* NY - Syracuse - Histotech


* NY - NYC -  Histotech (3rd shift)


* NY - Long Island - Histology Manager with Derm experience


* MI - Histology Manager with Derm experience


* OK - Histology Supervisor


* GA - Histology Supervisor


* NV - Histotech (3rd shift)


* TN - Histotech (1st shift)


* MA - Cape Cod - Histotech


* TX - Western - Histology Supervisor



 

Cytogenetics and Other Specialities:



* NY - Toxicology Technologist


* NY - Bone Marrow Technologist


* OR - Toxicology Technologist


* MA - Cytogenetics Technologist


* NJ - Northern - Cytogenetics Technologist


* MD - Cytogenetics Technologist


* NJ - Northern - FISH Supervisor



 



Pathologist Assistant:


* 

Re: [Histonet] IHC FILD

[Mark Turner]

Since it wasn't a requirement for my position, I took the exam for my own 
personal satisfaction.  I highly recommend taking it if you have the chance and 
not worrying about whether you will receive extra money.  Successfully passing 
the exam is a personal milestone and may not be recognized by your current 
employer, however it just might make a future one consider investing a little 
stronger in you because you have invested in yourself.

Good luck!

Mark Turner


 Debora Probst  wrote: 
> Can anyone tell me if once you have taken the IHC certification test and
> passed dose the administration consider that a specialty field and give
> you a pay increase? Or is it just for a persons own gratification to
> take it and pass? 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] IHC FILD

[Angela Bitting]

In my case, it was for my own personal gratification. 

>>> "Debora Probst"  10/15/2010 1:53 
>>> PM >>>
Can anyone tell me if once you have taken the IHC certification test and
passed dose the administration consider that a specialty field and give
you a pay increase? Or is it just for a persons own gratification to
take it and pass? 

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Re: [Histonet] IHC FILD

[BSullivan]

I have never received any additional pay for any of my certifications. It
is something I did for myself.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


   
 "Debora Probst"   
 To 
 Sent by:   
 histonet-bounces@  cc 
 lists.utsouthwest 
 ern.edu   Subject 
   [Histonet] IHC FILD 
   
 10/15/2010 01:53  
 PM
   
   
   




Can anyone tell me if once you have taken the IHC certification test and
passed dose the administration consider that a specialty field and give
you a pay increase? Or is it just for a persons own gratification to
take it and pass?

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[Histonet] IHC FILD

[Debora Probst]

Can anyone tell me if once you have taken the IHC certification test and
passed dose the administration consider that a specialty field and give
you a pay increase? Or is it just for a persons own gratification to
take it and pass? 

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RE: [Histonet] Locking up formalin

[Thomas Jasper]

Hi Victoria,

I've never heard of this, of course it could exist somewhere.  I wonder
what would prompt this.  There are obvious safety issues to consider.
Perhaps an unauthorized party, somewhere, got into some formalin and
caused problems?

Locked up or not, proper spill containment is mandated by OSHA (I
believe) so, again perhaps an unauthorized personnel issue?  

I've worked in the Upper Midwest and Pacific Northwest and have not
heard of this.  I regularly attend the NSH and have not heard anything
at the meetings either.

Thomas Jasper
Histology Supervisor
Central Oregon Regional Path

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Spoon,
Victoria
Sent: Thursday, October 14, 2010 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Locking up formalin

Is anyone aware of regulations stating that formalin has to be locked
up-  put in locked cabinets when not under direct supervision?

Applying to either clinics where specimens are collected into formalin
containers or in the pathology lab?

Thank you


Victoria Spoon
Anatomic Pathology Manager
Bassett Medical Center
Cooperstown NY 13326
victoria.sp...@bassett.org
Tel(607) 547-6357
Fax(607) 547-3203



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[Histonet] I

[Marilyn . A . Weiss]

I will be out of the office starting  10/14/2010 and will not return until
10/18/2010.

In my absence please ask for Mary Campbell .  If this is urgent or you need
to speak to me directly  you can contact me on my cell phone number
858-472-4266.
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RE: [Histonet] negative controls

[Kuhnla, Melissa]

Hi Vikki,
I have 1 Ventana XT and 3 Ultras.  I have certain antibodies designated
to each ultra.  This means not all slides from any case are guaranteed
to be on the same machine.  They obviously all have their own detection
kit.  My theory is that our detection kits are QCd prior to initial load
onto an instrument. The patient block was treated the same prior to
cutting. The slides receive solutions that are QCd...I think we are
covered. What are your thoughts?
Melissa
  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria
Baker
Sent: Friday, October 15, 2010 10:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls

Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run?  Also, if you are doing this and have to use a different detection
kit
how do you work the QA/QC portion of this for CAP requirements.

Thanks

Vikki
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[Histonet] AFB stain

[Amy Farnan]

Have any of you know  heard of  AFB will picking up in any other organisms 
beside mycobacterium? 
 
Amy

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[Histonet] Bone Marrow Trephines

[Vickroy, Jim]

I sent out an email around two months ago regarding a problem with our bone 
marrow trephines.   Our pathologist says that many of the trephines appear to 
be fragmented and although this has not prohibited determining a diagnosis it 
is still a problem.   Currently we use AZF as a fixative and decal the 
trephines in Rapid Immuno Cal by BBC.  We have investigated our times in each 
solution and have found that our hematopathologists often want us to rush the 
cases and the time in AZF is probably less than some other institutions.   We 
use a minimum time of 3 hours and try to have most of the biopsies fix for 
around 6 -8 hours.   Our standard of time in the decal solution is 1.5 hrs.  
(Variable depending on the amount of hard bone in the biopsy).

I have also wondered whether the "fragmentation" may be a result of the 
sectioning technique, time the sections float on the waterbath, or temperature 
of the waterbath.   We have not noticed this artifact in the routine tissues 
and on recutting sometimes the artifact is diminished but not completely gone.  
 Would anybody be willing to share with me their protocols so that we can 
continue to investigate a cause of the 'fragmentation"?   Any help would be 
appreciated.  Thanks

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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[Histonet] Update On New Job Openings

[Alyssa Peterson]

Allied Search Partners has been retained for the following searches.



   1. Please email a copy of updated resume to
   aly...@alliedsearchpartners.com for a full job description.
   2. Please send availability for a phone screen with one of our
   recruiters.
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   City/State



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* *

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SCHEDULE & DEPARTMENT:

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9PM-5:30AM Monday-Friday (Cytology Department)

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RE: [Histonet] negative controls

[Sebree Linda A]

Beatrice,

If there is not adequate tissue to cut a negative control, the pathologist has 
to make a "priority" decision so as to allow 1 slide to be treated as the 
negative control.  If for some reason, there is only 1 slide available, i.e. no 
slide available to use as a negative control, we do not run the test. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: bsulli...@shorememorial.org [mailto:bsulli...@shorememorial.org] 
Sent: Friday, October 15, 2010 11:01 AM
To: sgoe...@xbiotech.com
Cc: Histo Net list server; histonet-boun...@lists.utsouthwestern.edu; Sebree 
Linda A; Rene J Buesa
Subject: RE: [Histonet] negative controls

We're talking NEGATIVE controls here folks and if you use internal tissue
for your positive and negative controls...they should all be
processed and blocked in the same fashion as your actual patient material.
We have scrutinized the negative control issue on the CAP checklist and
have decided that if tissue warrants it, we cut an extra patient slide and
it is run as the negative for that case. All is processed the same and
stained identically with exception of the primary antibody. If there is not
adequate material  we use the Positive control and treat it negatively.
This is reflected in our policy and procedure.


Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


   
  
 Sent by:   To 
 histonet-bounces@ "Rene J Buesa"   
 lists.utsouthwest  cc 
 ern.edu   Histo Net list server   

   , Sebree Linda A
 10/15/2010 11:47
 AMSubject 
   RE: [Histonet] negative controls
   
   
   
   
   
   




So for every HP you do, you process a control cassette with the patient
tissue cassette?  That seems like alot?  How do you get that many
control tissues on a daily basis?  What do you do with the remaining
tissue in the control block?  If you throw them away everyday, I would
be interested in some of them.  How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time?  We have always just had a bunch of
blocks that you cut a control from?  I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue.  If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times.  Why couldn't you
just have one for all 3 cases?  Then the next day have a fresh ONE for
that day, date them, and file them.  So if you needed to see the HP
control for October 15th, you could go pull the control for that day...

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




 Original Message 
Subject: RE: [Histonet] negative controls
From: Rene J Buesa 
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A , sgoe...@xbiotech.com
Cc: Histo Net list server 

Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.

--- On Fri, 10/15/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" 
Cc: "Histo Net list server" 
Date: Friday, October 15, 2010, 11:17 AM


   Why do you need a negative control for each block if y

RE: [Histonet] negative controls

[Sebree Linda A]

To clarify even further:  we cut 2 patient sections, put one on a slide with a 
section of positive control already on it.  This slide gets stained with the 
antibody  The other section goes on another slide and is run as a corresponding 
negative control using the same antibody protocol but substituting a negative 
control serum for the antibody, thus this is a "negative reagent control" 
slide.  Elements within the patient slide that received antibody and are 
expected to be negative, serve as a "negative tissue control".  Again, we run 1 
negative control slide for EVERY 1 patient block in a run but only 1 negative 
control per any number of antibodies run on that same block, using the harshest 
protocol.  Only autopsy cases differ in that we run 1 negative control per 
TISSUE TYPE.


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com] 
Sent: Friday, October 15, 2010 10:48 AM
To: Rene J Buesa
Cc: Histo Net list server; Sebree Linda A
Subject: RE: [Histonet] negative controls

So for every HP you do, you process a control cassette with the patient
tissue cassette?  That seems like alot?  How do you get that many
control tissues on a daily basis?  What do you do with the remaining
tissue in the control block?  If you throw them away everyday, I would
be interested in some of them.  How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time?  We have always just had a bunch of
blocks that you cut a control from?  I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue.  If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times.  Why couldn't you
just have one for all 3 cases?  Then the next day have a fresh ONE for
that day, date them, and file them.  So if you needed to see the HP
control for October 15th, you could go pull the control for that day...

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




 Original Message 
Subject: RE: [Histonet] negative controls
From: Rene J Buesa 
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A , sgoe...@xbiotech.com
Cc: Histo Net list server 

Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J. 

--- On Fri, 10/15/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" 
Cc: "Histo Net list server" 
Date: Friday, October 15, 2010, 11:17 AM

 
   Why do you need a negative control for each block if you are runn=
ing
   the  same  antibody  on each patient block?  Is it just for case by c
  ase  reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all
the
   slides you d= id on that day, on that run, could be referenced back
to
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744

   =

   (512)386-= 5107

    Original Message 
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= o...@gmail.com>, "Histo Net
list
   server"
   <[3]histo...@lists.uts= outhwestern.edu>
   We run negative controls on every block of a case within the same
run.
   On autopsy cases, we only run 1 negative per tissue type, within the
   same run...this is the only exception to the rule of 1 negative per
   block.
   Linda A. Sebree
   University of Wisconsin Hospital & Clinics
   IHC/ISH Laboratory
   DB1-223 VAH
   600 Highland Ave.
   Madison, WI 53792
   (608)265-6596
   -Original Message-
   From: [4]histonet= -boun...@lists.utsouthwestern.edu
   [[5]mailto:histon=  et-boun...@lists.utsouthwestern.edu]  On Behalf
Of
   Victoria
   Baker
   Sent: Friday, October 15, 2010 9:26 AM
   To: Histo Net list server
   Subject: [Histonet] negative controls
   Hi
   I have a hypothetical question to 

RE: [Histonet] negative controls

[BSullivan]

We're talking NEGATIVE controls here folks and if you use internal tissue
for your positive and negative controls...they should all be
processed and blocked in the same fashion as your actual patient material.
We have scrutinized the negative control issue on the CAP checklist and
have decided that if tissue warrants it, we cut an extra patient slide and
it is run as the negative for that case. All is processed the same and
stained identically with exception of the primary antibody. If there is not
adequate material  we use the Positive control and treat it negatively.
This is reflected in our policy and procedure.


Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


   

 Sent by:   To
 histonet-bounces@ "Rene J Buesa" 
 lists.utsouthwest  cc
 ern.edu   Histo Net list server 
   
   , Sebree Linda A
 10/15/2010 11:47  
 AMSubject
   RE: [Histonet] negative controls
   
   
   
   
   
   




So for every HP you do, you process a control cassette with the patient
tissue cassette?  That seems like alot?  How do you get that many
control tissues on a daily basis?  What do you do with the remaining
tissue in the control block?  If you throw them away everyday, I would
be interested in some of them.  How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time?  We have always just had a bunch of
blocks that you cut a control from?  I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue.  If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times.  Why couldn't you
just have one for all 3 cases?  Then the next day have a fresh ONE for
that day, date them, and file them.  So if you needed to see the HP
control for October 15th, you could go pull the control for that day...

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




 Original Message 
Subject: RE: [Histonet] negative controls
From: Rene J Buesa 
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A , sgoe...@xbiotech.com
Cc: Histo Net list server 

Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.

--- On Fri, 10/15/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" 
Cc: "Histo Net list server" 
Date: Friday, October 15, 2010, 11:17 AM


   Why do you need a negative control for each block if you are runn=
ing
   the  same  antibody  on each patient block?  Is it just for case by c
  ase  reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all
the
   slides you d= id on that day, on that run, could be referenced back
to
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744

   =

   (512)386-= 5107

    Original Message 
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= o...@gmail.com>, "Histo Net
list
   server"
   <[3]histo...@lists.uts= outhwestern.ed

Re: [Histonet] negative controls

[Mark Tarango]

Hi Sarah,

It's better to have the control on the same slide.  There are slides that
work and slides that don't.  You know which ones are good and which ones are
bad because you have that control on each slide.  It's not always a complete
run that fails.  Yes, you CAN have a single batch control (it's not against
the rules), but best practice is to have a control on each slide.

You don't process a control for each case, you use a similarly processed
piece of positive tissue placed on each slide (except the negative which
should just have the patient tissue).


Mark
On Fri, Oct 15, 2010 at 8:47 AM,  wrote:

> So for every HP you do, you process a control cassette with the patient
> tissue cassette?  That seems like alot?  How do you get that many
> control tissues on a daily basis?  What do you do with the remaining
> tissue in the control block?  If you throw them away everyday, I would
> be interested in some of them.  How do you know what IHC stains the
> pathologist is going to order to know what control tissue to fix and
> process at the exact same time?  We have always just had a bunch of
> blocks that you cut a control from?  I understand that there is
> variability with processing, age, etc. not trying to be dense just still
> don't understand... Most places I have ever worked have control blocks
> that they cut a fresh control from everyday, then stain with the patient
> tissue.  If there are 3 HP cases, from what I am understanding, you guys
> are saying you need 3 controls for slides that are on the same machine,
> with the same reagents, same antibody, and same times.  Why couldn't you
> just have one for all 3 cases?  Then the next day have a fresh ONE for
> that day, date them, and file them.  So if you needed to see the HP
> control for October 15th, you could go pull the control for that day...
>
> Sarah Goebel, B.A., HT (ASCP)
> Histotechnician
>
>
> XBiotech USA Inc.
>
> 8201 East Riverside Dr. Bldg 4 Suite 100
>
> Austin, Texas  78744
>
> (512)386-5107
>
>
>
>
>  Original Message 
> Subject: RE: [Histonet] negative controls
>  From: Rene J Buesa 
> Date: Fri, October 15, 2010 8:33 am
> To: Sebree Linda A , sgoe...@xbiotech.com
> Cc: Histo Net list server 
>
> Because each tissue block has its own characteristics regarding fixation
> and processing some of which can influence the reactivity. If you have a
> bank of negative controls, how can you be sure that any of those blocks
> have received exactly the same treatment and reacted in the same way to
> the test block?
> The same goes for any bank of positives, so that is why you should have
> a positive control section in the same slide as the test section.
> René J.
>
> --- On Fri, 10/15/10, sgoe...@xbiotech.com  wrote:
>
>
> From: sgoe...@xbiotech.com 
> Subject: RE: [Histonet] negative controls
> To: "Sebree Linda A" 
> Cc: "Histo Net list server" 
> Date: Friday, October 15, 2010, 11:17 AM
>
>
>   Why do you need a negative control for each block if you are runn=
> ing
>   the  same  antibody  on each patient block?  Is it just for case by c
>  ase  reference  so  the negative is filed with the patient slide?  Why
>   co=  uldn't  you  have  a control slide bank that was dated so all
> the
>   slides you d= id on that day, on that run, could be referenced back
> to
>   that control? = ; Just curious?
>
>   Sarah Goebel, B.A., HT (ASCP)
>
>   Histotechnician<= br>
>
>   XBiotech USA Inc.
>
>   8201 East Riverside Dr. Bld= g 4 Suite 100
>
>   Austin, Texas  78744
>
>   =
>
>   (512)386-= 5107
>
>    Original Message 
>   Subject: RE: [Histonet] negative controls
>   From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
>   Date: Fri, October 15, 2010 8:08 am
>   To:  "Victoria  Baker"  <[2]bakevict= o...@gmail.com>, "Histo Net
> list
>   server"
>   <[3]histo...@lists.uts= outhwestern.edu>
>   We run negative controls on every block of a case within the same
> run.
>   On autopsy cases, we only run 1 negative per tissue type, within the
>   same run...this is the only exception to the rule of 1 negative per
>   block.
>   Linda A. Sebree
>   University of Wisconsin Hospital & Clinics
>   IHC/ISH Laboratory
>   DB1-223 VAH
>   600 Highland Ave.
>   Madison, WI 53792
>   (608)265-6596
>   -Original Message-
>   From: [4]histonet= -boun...@lists.utsouthwestern.edu
>   [[5]mailto:histon=  et-boun...@lists.utsouthwestern.edu]  On Behalf
> Of
>   Victoria
>   Baker
>   Sent: Friday, October 15, 2010 9:26 AM
>   To: Histo Net list server
>   Subject: [Histonet] negative controls
>   Hi
>   I have a hypothetical question to those who run IHC on Ventana
>   instruments.
>   Are you running your negatives with your patient/test cases or on a
>   separate
>   run? Also, if you are doing this and have to use a different
> detection
>   kit
>   how do you work the QA/QC portion of this for CAP requirements.
>   Thanks
>   Vikki
>   ___
>   Histonet mailing list
>   [6]h

RE: [Histonet] negative controls

[sgoebel]

So for every HP you do, you process a control cassette with the patient
tissue cassette?  That seems like alot?  How do you get that many
control tissues on a daily basis?  What do you do with the remaining
tissue in the control block?  If you throw them away everyday, I would
be interested in some of them.  How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time?  We have always just had a bunch of
blocks that you cut a control from?  I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue.  If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times.  Why couldn't you
just have one for all 3 cases?  Then the next day have a fresh ONE for
that day, date them, and file them.  So if you needed to see the HP
control for October 15th, you could go pull the control for that day...

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




 Original Message 
Subject: RE: [Histonet] negative controls
From: Rene J Buesa 
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A , sgoe...@xbiotech.com
Cc: Histo Net list server 

Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J. 

--- On Fri, 10/15/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" 
Cc: "Histo Net list server" 
Date: Friday, October 15, 2010, 11:17 AM

 
   Why do you need a negative control for each block if you are runn=
ing
   the  same  antibody  on each patient block?  Is it just for case by c
  ase  reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all
the
   slides you d= id on that day, on that run, could be referenced back
to
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744

   =

   (512)386-= 5107

    Original Message 
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= o...@gmail.com>, "Histo Net
list
   server"
   <[3]histo...@lists.uts= outhwestern.edu>
   We run negative controls on every block of a case within the same
run.
   On autopsy cases, we only run 1 negative per tissue type, within the
   same run...this is the only exception to the rule of 1 negative per
   block.
   Linda A. Sebree
   University of Wisconsin Hospital & Clinics
   IHC/ISH Laboratory
   DB1-223 VAH
   600 Highland Ave.
   Madison, WI 53792
   (608)265-6596
   -Original Message-
   From: [4]histonet= -boun...@lists.utsouthwestern.edu
   [[5]mailto:histon=  et-boun...@lists.utsouthwestern.edu]  On Behalf
Of
   Victoria
   Baker
   Sent: Friday, October 15, 2010 9:26 AM
   To: Histo Net list server
   Subject: [Histonet] negative controls
   Hi
   I have a hypothetical question to those who run IHC on Ventana
   instruments.
   Are you running your negatives with your patient/test cases or on a
   separate
   run? Also, if you are doing this and have to use a different
detection
   kit
   how do you work the QA/QC portion of this for CAP requirements.
   Thanks
   Vikki
   ___
   Histonet mailing list
   [6]histo...@lists.utsouth= western.edu
   [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
   ___
   Histonet mailing list
   [8]histo...@lists.utsouth= western.edu
   [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet

References

   1. 3D"mailto:lseb...@uwhealth.org";
   2. 3D"mailto:bakevicto...@gmail.com";
   3. 3D"mailto:HistoNet@lists.utsouthwestern.edu";
   4. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
   5. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
   6. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
   8. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"

RE: [Histonet] negative controls

[Rene J Buesa]

Because each tissue block has its own characteristics regarding fixation and 
processing some of which can influence the reactivity. If you have a bank of 
negative controls, how can you be sure that any of those blocks have received 
exactly the same treatment and reacted in the same way to the test block?
The same goes for any bank of positives, so that is why you should have a 
positive control section in the same slide as the test section.
René J. 

--- On Fri, 10/15/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" 
Cc: "Histo Net list server" 
Date: Friday, October 15, 2010, 11:17 AM



   Why do you need a negative control for each block if you are runn= ing
   the  same  antibody  on each patient block?  Is it just for case by c   ase  
reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all the
   slides you d= id on that day, on that run, could be referenced back to
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744

   =

   (512)386-= 5107

    Original Message 
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= o...@gmail.com>, "Histo Net list
   server"
   <[3]histo...@lists.uts= outhwestern.edu>
   We run negative controls on every block of a case within the same run.
   On autopsy cases, we only run 1 negative per tissue type, within the
   same run...this is the only exception to the rule of 1 negative per
   block.
   Linda A. Sebree
   University of Wisconsin Hospital & Clinics
   IHC/ISH Laboratory
   DB1-223 VAH
   600 Highland Ave.
   Madison, WI 53792
   (608)265-6596
   -Original Message-
   From: [4]histonet= -boun...@lists.utsouthwestern.edu
   [[5]mailto:histon=  et-boun...@lists.utsouthwestern.edu]  On Behalf Of
   Victoria
   Baker
   Sent: Friday, October 15, 2010 9:26 AM
   To: Histo Net list server
   Subject: [Histonet] negative controls
   Hi
   I have a hypothetical question to those who run IHC on Ventana
   instruments.
   Are you running your negatives with your patient/test cases or on a
   separate
   run? Also, if you are doing this and have to use a different detection
   kit
   how do you work the QA/QC portion of this for CAP requirements.
   Thanks
   Vikki
   ___
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   [8]histo...@lists.utsouth= western.edu
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References

   1. 3D"mailto:lseb...@uwhealth.org";
   2. 3D"mailto:bakevicto...@gmail.com";
   3. 3D"mailto:HistoNet@lists.utsouthwestern.edu";
   4. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
   5. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
   6. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
   8. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
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RE: [Histonet] negative controls

[sgoebel]

   Why do you need a negative control for each block if you are runn= ing
   the  same  antibody  on each patient block?  Is it just for case by c   ase  
reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all the
   slides you d= id on that day, on that run, could be referenced back to
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744

   
   (512)386-= 5107

    Original Message 
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]lseb...@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= o...@gmail.com>, "Histo Net list
   server"
   <[3]histo...@lists.uts= outhwestern.edu>
   We run negative controls on every block of a case within the same run.
   On autopsy cases, we only run 1 negative per tissue type, within the
   same run...this is the only exception to the rule of 1 negative per
   block.
   Linda A. Sebree
   University of Wisconsin Hospital & Clinics
   IHC/ISH Laboratory
   DB1-223 VAH
   600 Highland Ave.
   Madison, WI 53792
   (608)265-6596
   -Original Message-
   From: [4]histonet= -boun...@lists.utsouthwestern.edu
   [[5]mailto:histon=  et-boun...@lists.utsouthwestern.edu]  On Behalf Of
   Victoria
   Baker
   Sent: Friday, October 15, 2010 9:26 AM
   To: Histo Net list server
   Subject: [Histonet] negative controls
   Hi
   I have a hypothetical question to those who run IHC on Ventana
   instruments.
   Are you running your negatives with your patient/test cases or on a
   separate
   run? Also, if you are doing this and have to use a different detection
   kit
   how do you work the QA/QC portion of this for CAP requirements.
   Thanks
   Vikki
   ___
   Histonet mailing list
   [6]histo...@lists.utsouth= western.edu
   [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
   ___
   Histonet mailing list
   [8]histo...@lists.utsouth= western.edu
   [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet

References

   1. 3D"mailto:lseb...@uwhealth.org";
   2. 3D"mailto:bakevicto...@gmail.com";
   3. 3D"mailto:HistoNet@lists.utsouthwestern.edu";
   4. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
   5. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
   6. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
   8. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
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RE: [Histonet] negative controls

[Sebree Linda A]

We run negative controls on every block of a case within the same run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria
Baker
Sent: Friday, October 15, 2010 9:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls

Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run?  Also, if you are doing this and have to use a different detection
kit
how do you work the QA/QC portion of this for CAP requirements.

Thanks

Vikki
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Re: [Histonet] negative controls

[Mark Tarango]

Hi Vikki,

It's best to run it all together and run a negative control for each
detection kit.

Mark
On Fri, Oct 15, 2010 at 7:25 AM, Victoria Baker wrote:

> Hi
> I have a hypothetical question to those who run IHC on Ventana instruments.
> Are you running your negatives with your patient/test cases or on a
> separate
> run?  Also, if you are doing this and have to use a different detection kit
> how do you work the QA/QC portion of this for CAP requirements.
>
> Thanks
>
> Vikki
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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[Histonet] FT HT needed in Fayetteville, NC

[Wendy Bridges]

We're more than a job...we're a family.

Cape Fear Valley Health is a regional health system serving a six-county
region of Southeastern North Carolina, with more than 935,000 patient
visits annually. A private not-for-profit organization and the state's
ninth largest health system, it includes: Cape Fear Valley Medical
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Rehabilitation Center, Behavioral Health Care and Bladen County
Hospital. We are centrally located, in a diverse community of 300,000
residents, just two hours from North Carolina's pristine beaches and
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877-7-CAPEFEAR or you can apply online at www.capefearcareers.com
 .

 

*   We're looking for HISTOTECHNOLOGIST/HISTOTECHNICIAN to work in
our busy, state-of-the art CAP accredited laboratory. Bachelor or
Associate degree in relevant science and appropriate clinical training
in the testing of neonatal, pediatric, adolescent and adult patients.
HTL (ASCP)/HT (ASCP) or equivalent registration.

 

If you're interested in a dynamic team environment where career growth
is optimum or to learn more about benefits and career opportunities,
visit us at www.capefearvalley.com
 .

 

EEO/AAP Employer

 

CONFIDENTIALITY NOTICE: This electronic mail transmission may contain 
information that is privileged and/or confidential. Additionally, this 
communication may contain individual protected health information ("PHI") that 
is subject to protection under state and federal laws, or other privileged, 
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RE: [Histonet] negative controls

[sgoebel]

Pretty sure you have to run your positive with your negative to keep the
conditions 100% the same.  You don't have to put them on the same slide
necessarily, but they need to be on the same run.  Also, say you are
running 15 HP patient slides, you can have one negative control for all
of these as long as it is on the same run.

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




 Original Message 
Subject: [Histonet] negative controls
From: Victoria Baker 
Date: Fri, October 15, 2010 7:25 am
To: Histo Net list server 

Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run? Also, if you are doing this and have to use a different detection
kit
how do you work the QA/QC portion of this for CAP requirements.

Thanks

Vikki
___
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RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20

[Weems, Joyce]

http://www.flpath.org/rli2.asp

Here is a link that will help..  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Friday, October 15, 2010 09:59
To: Weems, Joyce
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20

Can you please provide the specific CMS update number?  The website doesn't 
seem to be too user friendly ...   thanks!



On Oct 14, 2010, at 12:22 PM, "Weems, Joyce"  wrote:

> 
> CMS/NCCI Update Dated October 1, 2009
> 
> 8. The unit of service for special stains (CPT codes 88312-88313) and 
> immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If 
> it is medically reasonable and necessary to perform the same stain on 
> more than one specimen or more than one block of tissue from the same 
> specimen, additional units of service may be reported for the 
> additional specimen(s) or block(s). Physicians should not report more 
> than one unit of service for a stain performed on a single tissue 
> block. For example it is common practice to cut multiple levels from a 
> tissue block and stain each level with the same stain. The multiple 
> levels from the same block of tissue stained with the same stain 
> should not be reported as additional units of service. Only one unit 
> of service should be reported for the stain on multiple levels from 
> the single tissue block. Additionally, controls performed with special 
> stains should not be reported as separate units of service for the 
> stain.
> 
> 
> -Original Message-
> From: Mike Pence [mailto:mpe...@grhs.net]
> Sent: Thursday, October 14, 2010 11:31
> To: Weems, Joyce; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
> 
> Can you site your source, please.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, 
> Joyce
> Sent: Thursday, October 14, 2010 10:25 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
> 
> 
> 
> 
> The change is that you can bill per block now and not per specimen. This is 
> for immunos and special stains. It does make a huge difference! 
> 
> Best,
> 
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chris 
> Evanish
> Sent: Thursday, October 14, 2010 11:10
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
> 
> Has anyone heard of a cpt coding change that allows us to bill 88342 per 
> slide run instead of per antibody? One of our Pathologist was at a conference 
> and was told that we could do that. It makes a big difference with running 
> cytokeratins on multiple blocks and levels of sentinel nodes.
> 
>  Thanks,
> Chris Evanish
> Montgomery Hospital
> Norristown PA
> 
> Chris D. Evanish
> Histology Supervisor
> Montgomery Hospital
> 610-270-2379
> 
> Please consider the environment before printing this email " to your outgoing 
> mail. 
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Catholic Health 
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> It may contain information that is privileged and confidential.  Any 
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> are not the intended recipient, please delete this message, and reply to the 
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Re: [Histonet] negative controls

[BSullivan]

To my knowledge for this stain to be legitimate and meet criteria  you must
run your positive and negative controls on the same run.


Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


   
 Victoria Baker
 To 
 Sent by:  Histo Net list server   
 histonet-bounces@  
 lists.utsouthwest  cc 
 ern.edu   
   Subject 
   [Histonet] negative controls
 10/15/2010 10:25  
 AM
   
   
   
   




Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with your patient/test cases or on a
separate
run?  Also, if you are doing this and have to use a different detection kit
how do you work the QA/QC portion of this for CAP requirements.

Thanks

Vikki
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[Histonet] negative controls

[Victoria Baker]

Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with your patient/test cases or on a separate
run?  Also, if you are doing this and have to use a different detection kit
how do you work the QA/QC portion of this for CAP requirements.

Thanks

Vikki
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Re: [Histonet] Optimal processing for prostate needle biopsies

[Phyllis Thaxton]

Nilesh,
 This doesn't sound like a staining problem, it sounds like a processing 
problem. Try preprocessing for 5 minutes instead of 15 and see if that 
helps. OR 
what we had to do with GI biopsies, we stopped using any preprocessing with 
them. After fixation, they are placed in molecular fixative for 10 minutes 
before processing. 

  If that doesn't work, I would investigate further to where the biopsy was 
actually obtained. We had a problem with urologists obtaining the core, then 
swishing the needle gun around in a container of saline, then ADDING formalin 
to 
the saline after the biopsy was obtained. The biopsies were horrible.
  Hope this helps.
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 





From: "Gupta, Nilesh" 
To: Phyllis Thaxton ; "histonet@lists.utsouthwestern.edu" 

Sent: Thu, October 14, 2010 8:08:17 AM
Subject: RE: [Histonet] Optimal processing for prostate needle biopsies


Phyllis, 
We hold all our needle cores overnight and pre-process for 15 min. The problems 
we are having is shrunken nuclei and nucleoli are not distinct even in 
carcinoma 
cases. Cracking artifacts of cores (longitudinal), darker and paler staining 
along the length of the cores. We tried Harris but staining with Mayer's brings 
out nucleoli better.
 
 


From: Phyllis Thaxton [dch...@yahoo.com]
Sent: Wednesday, October 13, 2010 10:37 AM
To: Gupta, Nilesh; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Optimal processing for prostate needle biopsies


How long are you pre-processing the prostate biopsies before processing on the 
XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, 
then wash for 20 minutes, pre-processing solution for 10 minutes then process. 
We use Harris Hematoxylin in our H&E and morphology is good.
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 





From: "Gupta, Nilesh" 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Tue, October 12, 2010 12:02:23 PM
Subject: [Histonet] Optimal processing for prostate needle biopsies

I have a few questions regrading processing of prostate needle biopsies.
1.  What is optimal fixation time that the needle cores should be fixed for 
before loading these on the processors.
2.  Our cores are processed on Tissue tek Xpress x120 but the morphology is not 
so good. I'd like to know if any other labs have tried this processor for 
prostate needle cores processing and would like to know their experience as far 
as morphologic quality on slides
3.  What is the best H&E stain to use for prostate needle biopsies. We are 
currently using Mayer's which is giving us better staining than Harris. Any 
recommendations on which H&E is best suited for prostate needle biopsies.

Thanks

N.Gupta
Henry Ford Hospital

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Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20

[histot...@imagesbyhopper.com]

Can you please provide the specific CMS update number?  The website doesn't 
seem to be too user friendly ...   thanks!



On Oct 14, 2010, at 12:22 PM, "Weems, Joyce"  wrote:

> 
> CMS/NCCI Update Dated October 1, 2009 
> 
> 8. The unit of service for special stains (CPT codes 88312-88313) and
> immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If
> it is medically reasonable and necessary to perform the same stain on
> more than one specimen or more than one block of tissue from the same
> specimen, additional units of service may be reported for the
> additional specimen(s) or block(s). Physicians should not report more
> than one unit of service for a stain performed on a single tissue
> block. For example it is common practice to cut multiple levels from a
> tissue block and stain each level with the same stain. The multiple
> levels from the same block of tissue stained with the same stain
> should not be reported as additional units of service. Only one unit
> of service should be reported for the stain on multiple levels from
> the single tissue block. Additionally, controls performed with special
> stains should not be reported as separate units of service for the
> stain. 
> 
> 
> -Original Message-
> From: Mike Pence [mailto:mpe...@grhs.net] 
> Sent: Thursday, October 14, 2010 11:31
> To: Weems, Joyce; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
> 
> Can you site your source, please.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
> Sent: Thursday, October 14, 2010 10:25 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
> 
> 
> 
> 
> The change is that you can bill per block now and not per specimen. This is 
> for immunos and special stains. It does make a huge difference! 
> 
> Best, 
> 
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax 
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chris Evanish
> Sent: Thursday, October 14, 2010 11:10
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
> 
> Has anyone heard of a cpt coding change that allows us to bill 88342 per 
> slide run instead of per antibody? One of our Pathologist was at a conference 
> and was told that we could do that. It makes a big difference with running 
> cytokeratins on multiple blocks and levels of sentinel nodes.
> 
>  Thanks,
> Chris Evanish
> Montgomery Hospital
> Norristown PA
> 
> Chris D. Evanish
> Histology Supervisor
> Montgomery Hospital
> 610-270-2379 
> 
> Please consider the environment before printing this email " to your outgoing 
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RE: [Histonet] Immunohistochemistry images

[sgoebel]

   Are  you  wanting  to  save your specific images, or just need a refe   
rence  guide  for  QC?  Photobucket or Snapfish are two sites that are
   pr= etty cheap if not free?

   Sarah Goebel, B.A., HT (ASCP)

   Histotec= hnician

   XBiotech USA Inc.

   <= /div>
   8201 East Riverside= Dr. Bldg 4 Suite 100
   Austin, Texas  78744
   (= 512)386-5107

    Original Message 
   Subject: RE: [Histonet] Immunohistochemistry images
   From: "Blazek, Linda" <[1]lbla...@digestivespecialists.com>
   Date: Thu, October 14, 2010 9:04 am
   To: 'Michele Carr' <[2]micheleca= r...@yahoo.com>,
   "[3]histo...@lists.utsout= hwestern.edu" <[4]   
histonet@lists.utsouthwestern.edu>
   Try the web site of the vender that you get your antibodies from.
   -Original Message-
   From:  [5]histonet=  -boun...@lists.utsouthwestern.edu
   [[6]mailto:histonet-boun...@lists.utsouthwestern.edu]   On  Behalf  Of
   Michele Carr
   Sent: Thursday, October 14, 2010 11:41 AM
   To: [7]histo...@lists.uts= outhwestern.edu
   Subject: [Histonet] Immunohistochemistry images
   Hi  everyone,  I  was wondering if anyone knew of a website that I can
   view an= d
   save  the  IHC images.  We are putting together a new procedure manual
   a= nd the
   pathogists  want images of the antibodies we use to be included in the
   manua= l.
   Thanks in advance for your responses.
   Michele Carr HTL ASCP
   Medical Laboratory Services
   Murrieta Ca
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References

   1. 3D"mailto:lbla...@digestivespecialists.co   2. 
3D"mailto:michelecar...@yahoo.com";
   3. 3D"mailto:histonet@lists.utsouthwestern.edu";
   4. 3D"mailto:histonet@lists.utsouthwestern.edu";
   5. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
   6. 3D"mailto:histonet-boun...@l   7. 
3D"mailto:histonet@lists.utsouthwestern.edu";
   8. 3D"mailto:Histonet@lists.utsouthwestern.edu";
   9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
  10. 3D"mailto:Histonet@lists.utsouthwestern.edu";
  11. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
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Re: [Histonet] I have a couple of quick questions

[Rene J Buesa]

Yes, xylene is the way to go.
No, 37ºC will not hurt the sections no matter for how long they are at that 
temp.
René J.

--- On Thu, 10/14/10, Lewis, Patrick  wrote:


From: Lewis, Patrick 
Subject: [Histonet] I have a couple of quick questions
To: Histonet@lists.utsouthwestern.edu
Date: Thursday, October 14, 2010, 7:11 PM


If I want to remove a cover slip that is coversliped with permount.  Do
I need to use xylene to get rid of the semi hardened permount gunk?



Also,  If I am heating paraffin sectioned slides overnight at 37C to
adhere them what happens if I over cook them by leaving them at 37C for
more than one day?



Say 3-4 days at 37C  It probably wont hurt them as they haven't been
epitope retrieved yet.















Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

206-884-1115  OFFICE 

000-000-  PAGER

000-000-  CELL 

206-884-7311  FAX

patrick.le...@seattlechildrens.org

OFFICE  1900 9th Avenue Seattle, WA 98101

MAIL      M/S C9S-8, Seattle, WA 98101

WWW     seattlechildrens.org  





CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, is for 
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privileged information protected by law.  Any unauthorized review, use, 
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Re: [Histonet] I have a couple of quick questions

[BSullivan]

Yes. to remove mounting media you need to leave them in the Xylene
after the coverslip is removed. If the mounting media is not totally
removed your stain, whatever one you are doing, will not give you the
results you need.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


   
 "Lewis, Patrick"  
  
 Sent by:   cc 
 histonet-bounces@ 
 lists.utsouthwest Subject 
 ern.edu   [Histonet] I have a couple of quick 
   questions   
   
 10/14/2010 07:11  
 PM
   
   
   




If I want to remove a cover slip that is coversliped with permount.  Do
I need to use xylene to get rid of the semi hardened permount gunk?



Also,  If I am heating paraffin sectioned slides overnight at 37C to
adhere them what happens if I over cook them by leaving them at 37C for
more than one day?



Say 3-4 days at 37C  It probably wont hurt them as they haven't been
epitope retrieved yet.















Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

206-884-1115  OFFICE

000-000-  PAGER

000-000-  CELL

206-884-7311  FAX

patrick.le...@seattlechildrens.org

OFFICE  1900 9th Avenue Seattle, WA 98101

MAIL  M/S C9S-8, Seattle, WA 98101

WWW seattlechildrens.org 





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for the sole use of the intended recipient(s) and may contain confidential
and privileged information protected by law.  Any unauthorized review, use,
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recipient, please contact the sender by reply e-mail and destroy all copies
of the original message.

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[Histonet] Invitation to connect on LinkedIn

[Tyrone Genade via LinkedIn]

LinkedIn
Tyrone Genade requested to add you as a connection on LinkedIn:
--

Jackie,

I'd like to add you to my professional network on LinkedIn.

- Tyrone

Accept invitation from Tyrone Genade
http://www.linkedin.com/e/yvpgd1-gfarewfb-54/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I901563316_3/pmpxnSRJrSdvj4R5fnhv9ClRsDgZp6lQs6lzoQ5AomZIpn8_cRYScjcPdzkNc3B9bRATu3tIkSBibPgUejcUdjcUdP4LrCBxbOYWrSlI/EML_comm_afe/

View invitation from Tyrone Genade
http://www.linkedin.com/e/yvpgd1-gfarewfb-54/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I901563316_3/0PnPoNcPcSdj4MekALqnpPbOYWrSlI/svi/
 
--

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LinkedIn? Enter the company name and years of employment or the prospective 
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you with a more balanced set of feedback to evaluate that new hire.
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