[Histonet] THANK YOU !!!!

[Lin Bustamante]

To all of you for the answer to my questions concerning 100 um thick
plastic sections and Absolute v/s absolute+methanol.
Thanks again
Lin.

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Frozen Section TAT

[histot...@imagesbyhopper.com]

Allison, 

I have created an excel spreadsheet where you put time started, time completed 
& it will calculate the amount time spent doing the frozen. It highlights any 
time grater than 20 min.  Let me know if you are interested in something like 
that.

 Michelle



On Nov 1, 2010, at 2:54 PM, "Scott, Allison D"  
wrote:

> Hello to all in histoland.  How is everyone calculating the FS TAT.
> Does any anyone have a file that automatically calculates that tat when
> you plug in the data, that they would be willing to share.  Any help
> would be appreciated.  
> 
> Allison Scott
> Histology Supervisor
> LBJ Hospital.
> CONFIDENTIALITY NOTICE:
> If you have received this e-mail in error, please immediately notify the
> sender by return e-mail and delete this e-mail and any attachments from 
> your computer system.
> 
> To the extent the information in this e-mail and any attachments contain 
> protected health information as defined by the Health Insurance Portability 
> and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
> 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
> privileged.  This e-mail may also be confidential and/or privileged under 
> Texas law.  The e-mail is for the use of only the individual or entity named 
> above.  If you are not the intended recipient, or any authorized 
> representative of the intended recipient, you are hereby notified that any 
> review, dissemination or copying of this e-mail and its attachments is 
> strictly prohibited.
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HT/HTL Opening Tualatin, Oregon

[Konni Black]

An experienced HT or HTL is needed for a new histology lab in Tualatin, Oregon. 
Great company, very flexible schedule, excellent pay. Please contact Konni 
Black if you are interested. kbl...@digestivehlth.com or 253-503-2560.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Frozen Section TAT

[Rene J Buesa]

Under separate cover I am sending something on this subject.
René J.

--- On Mon, 11/1/10, Scott, Allison D  wrote:


From: Scott, Allison D 
Subject: [Histonet] Frozen Section TAT
To: histonet@lists.utsouthwestern.edu
Date: Monday, November 1, 2010, 2:54 PM


Hello to all in histoland.  How is everyone calculating the FS TAT.
Does any anyone have a file that automatically calculates that tat when
you plug in the data, that they would be willing to share.  Any help
would be appreciated.  

Allison Scott
Histology Supervisor
LBJ Hospital.
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] IHC Staining Problem

[Ross Benik]

We have developed a staining protocol for the CD79a antibody from two
different vendors and both require a HIER TRIS/EDTA pH 9.0 retrieval. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Monday, November 01, 2010 2:34 PM
To: jh...@vet.k-state.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Staining Problem

Hi,
 I think you mentioned using citrate retrieval. Have you tried high
pH
retrieval with EDTA or an equivalent? I think CD79 works better with
high pH
retrieval.

Amos


Message: 1
Date: Sun, 31 Oct 2010 19:29:59 +
From: Jennifer Hill 
Subject: RE: [Histonet] IHC Staining Problem
To: histonet 
Message-ID:
 
<8aa2173dc209ca438077a832ff98bd7f02806...@vetmxht.ads.vet.k-state.edu
>
Content-Type: text/plain; charset="iso-8859-1"

Thanks to everyone who has responded so far.  To answer the questions
asked,
our controls are either being cut the same day or the day or two before,
so
they are not setting for extended periods.  We have also used at least
three
or four different controls, all with the same results. (The controls
stain
fine for other tests: CD20, CD3, etc.)The pathologists are saying that
the
patient slides are not staining as well.  So it doesn't seem to be
something
so simple as a control issue.  As to the dehydration/deparafinization,
that
takes place on an automated stainer, and no other stains are having
issues
so I don't believe that is the issue either.


Jennifer Hill
Kansas State University
Veterinary Diagnostic Lab
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC Staining Problem

[Amos Brooks]

Hi,
 I think you mentioned using citrate retrieval. Have you tried high pH
retrieval with EDTA or an equivalent? I think CD79 works better with high pH
retrieval.

Amos


Message: 1
Date: Sun, 31 Oct 2010 19:29:59 +
From: Jennifer Hill 
Subject: RE: [Histonet] IHC Staining Problem
To: histonet 
Message-ID:
   <8aa2173dc209ca438077a832ff98bd7f02806...@vetmxht.ads.vet.k-state.edu
>
Content-Type: text/plain; charset="iso-8859-1"

Thanks to everyone who has responded so far.  To answer the questions asked,
our controls are either being cut the same day or the day or two before, so
they are not setting for extended periods.  We have also used at least three
or four different controls, all with the same results. (The controls stain
fine for other tests: CD20, CD3, etc.)The pathologists are saying that the
patient slides are not staining as well.  So it doesn't seem to be something
so simple as a control issue.  As to the dehydration/deparafinization, that
takes place on an automated stainer, and no other stains are having issues
so I don't believe that is the issue either.


Jennifer Hill
Kansas State University
Veterinary Diagnostic Lab
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Frozen Section TAT

[Victor Tobias]

Allison,

We have 2 fields in the database for when the frozen specimen was 
received and when the surgeon was notified. We can then run a report to 
show us the cases with frozens and the TAT.


Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


On 11/1/2010 11:54 AM, Scott, Allison D wrote:

Hello to all in histoland.  How is everyone calculating the FS TAT.
Does any anyone have a file that automatically calculates that tat when
you plug in the data, that they would be willing to share.  Any help
would be appreciated.

Allison Scott
Histology Supervisor
LBJ Hospital.
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
your computer system.

To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged.  This e-mail may also be confidential and/or privileged under
Texas law.  The e-mail is for the use of only the individual or entity named
above.  If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Frozen Section TAT

[Weems, Joyce]

Our pathologist dictate the times and we review it manually for 100% of the 
frozens!! J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Monday, November 01, 2010 14:55
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Section TAT

Hello to all in histoland.  How is everyone calculating the FS TAT.
Does any anyone have a file that automatically calculates that tat when you 
plug in the data, that they would be willing to share.  Any help would be 
appreciated.  

Allison Scott
Histology Supervisor
LBJ Hospital.
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender 
by return e-mail and delete this e-mail and any attachments from your computer 
system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability and 
Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or 
Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under Texas 
law.  The e-mail is for the use of only the individual or entity named above.  
If you are not the intended recipient, or any authorized representative of the 
intended recipient, you are hereby notified that any review, dissemination or 
copying of this e-mail and its attachments is strictly prohibited.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please delete this message, and 
reply to the sender regarding the error in a separate email.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Frozen Section TAT

[Rathborne, Toni]

We have recently started using our LIS to help with this. When the specimen is 
accessioned (in Cerner Millennium) as a frozen section, tasks are generated. 
Additional tasks can be added depending on how many slides or additional blocks 
are prepared for the FS. These tasks can then be verified when complete (i.e. 
when the pathologist calls the results to the OR). All of this is heavily 
relied upon by the person entering the tasks accurately and in a timely manner. 
 We have only started this process this week, so it will be interesting 
watching the trends. Reports can then be generated monthly and by pathologist.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Scott,
Allison D
Sent: Monday, November 01, 2010 2:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Section TAT


Hello to all in histoland.  How is everyone calculating the FS TAT.
Does any anyone have a file that automatically calculates that tat when
you plug in the data, that they would be willing to share.  Any help
would be appreciated.  

Allison Scott
Histology Supervisor
LBJ Hospital.
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Frozen Section TAT

[Scott, Allison D]

Hello to all in histoland.  How is everyone calculating the FS TAT.
Does any anyone have a file that automatically calculates that tat when
you plug in the data, that they would be willing to share.  Any help
would be appreciated.  

Allison Scott
Histology Supervisor
LBJ Hospital.
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Standard work

[Kuhnla, Melissa]

Hi Marcia,
We are a core lab...doing the work of four hospitals.  We have been live
with Vantage (Ventana) for about nine months.  We have 3 symphonies, 3
ultras, 1 XT, and two nexus special stainers all tied into the system.
I would be happy to answer specifics for you.  We are happy. Ventana
seems good about working with us as far as current and future needs that
the system can not meet at this time.  In particular with regards to
shipping of specimens, cassettes and slides from one facility to
another.  Let me know if you have questions.
Melissa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia
Funk
Sent: Monday, November 01, 2010 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Standard work

 
Hist 
We are looking at tracking system and would like some additional
data
from labs that are tracking standard work for sectioning, embedding,
labeling.
Also special stains and IHC would be helpful.  Like to compare our
lab with other
labs to see if we are in the ballpark.  Appreciate any help you
might share.
Marcia
 
 
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail, and any attachments therein, is confidential 
and for use by the intended addressee only. If this message is received by you 
in error please do not disseminate or read further. Please reply to the sender 
that you have received the message in error, then delete the message. Although 
Catholic Health Services of Long Island attempts to sweep e-mail  and 
attachments for viruses, it does not guarantee that either are virus-free and 
accepts no liability for any damage sustained as a result of viruses. Thank you.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Standard work

[Mahoney,Janice A]

Hi Marcia,
I'd be glad to help but could you be more specific as to what you need?  We 
have the Ventana Vangage system and love it.  We are a Lean Lab as I know you 
are and I think this system fits in perfectly. Please feel free to contact me 
for specifics.
We have Job guidance sheets (i.e. procedures) that follow step by step how to 
use the system during each of the stations for Vantage.  Everyone does it the 
same way every time and we have only had 2 numbering errors in 2 years with 
Vantage.  Standard work and single piece flow are essential.  We post the flow 
at each station and do audits to assure compliance.  Everyone loves the system 
and feels much less stress.
>From the Vantage data I am able to collect I know my average cutting time per 
>block is just over 2 minutes and embedding is less than a minute.
Let me know what additional info you need.
Jan Mahoney
Omaha, NE

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Funk
Sent: Monday, November 01, 2010 12:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Standard work


Hist
We are looking at tracking system and would like some additional data
from labs that are tracking standard work for sectioning, embedding, 
labeling.
Also special stains and IHC would be helpful.  Like to compare our lab with 
other
labs to see if we are in the ballpark.  Appreciate any help you might share.
Marcia


Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is 
faithful to the healing ministry of Jesus Christ, providing high quality care 
for the body, mind and spirit of every person.

The information contained in this communication, including attachments, is 
confidential and private and intended only for the use of the addressees.  
Unauthorized use, disclosure, distribution or copying is strictly prohibited 
and may be unlawful.  If you received this communication in error, please 
inform us of the erroneous delivery by return e-mail message from your 
computer.  Additionally, although all attachments have been scanned at the 
source for viruses, the recipient should check any attachments for the presence 
of viruses before opening.  Alegent Health accepts no liability for any damage 
caused by any virus transmitted by this e-mail.  Thank you for your cooperation.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Standard work

[Marcia Funk]

 
Hist 
We are looking at tracking system and would like some additional data
from labs that are tracking standard work for sectioning, embedding, 
labeling.
Also special stains and IHC would be helpful.  Like to compare our lab with 
other
labs to see if we are in the ballpark.  Appreciate any help you might share.
Marcia
 
 
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC Staining Problem

[Reynolds,Donna M]

>
>We had a similar problem a few years back. We had used an antibody at 1:500 
>dilution for years. Got in a new bottle and no labeling. Called the 
>manufacture and they assured me it was the same concentration.  Finally went 
>to 1:50 dilution and the staining was like our old stain.  Over the last few 
>years with each new vial I titer and we are now up to 1:200 dilution. So I 
>suspect it is your antibody.  When the problem began I still had enough of the 
>old vial to run them simultaneously and the old vial was working fine. 
> --
>
> Message: 10
> Date: Fri, 29 Oct 2010 22:44:58 -0700
> From: Jennifer MacDonald 
> Subject: Re: [Histonet] IHC Staining Problem
> To: Jennifer Hill 
> Cc: histonet ,
>histonet-boun...@lists.utsouthwestern.edu
> Message-ID:
> >
> Content-Type: text/plain; charset="US-ASCII"
>
> How far in advance are your controls being cut?  Are your patient  
> tissues
> staining?
>
>
>
>
> Jennifer Hill 
> Sent by: histonet-boun...@lists.utsouthwestern.edu
> 10/29/2010 01:11 PM
>
> To
> histonet 
> cc
>
> Subject
> [Histonet] IHC Staining Problem
>
>
>
>
>
>
> I hope someone can help me with a problem I've been having with my  
> CD79a
> hand stain.  This is a stain that was validated and working perfectly,
> when a few months ago I started having reduced, to no staining on my
> controls, with the occasional run staining a little stronger. I  
> followed
> the discussion not too long ago with the person who was having similar
> problems, but the suggestions didn't seem to apply (I use Tween 20  
> in my
> PBS rinses, and we use APEX slides from Surgipath-not Fischer  
> Plus).  I've
> tried everything I can think of and I hope someone may have some other
> ideas.  For reference this is for a veterinary diagnostic lab, not a  
> human
> lab, and this is the only stain we've been having this issue with.
>
> My protocol is as follows: After deparfinization/rehydration,
> AR for 20 min in the steamer in Biogenex Citra Solution (I've gotten  
> new
> Citra Solution, tried a pressure cooker)
> 5 min peroxide quench, followed by a 10 min protein block using Dako's
> Protein Block-Serum Free
> 60 min Primary incubation (Dako CD79a) at 37 C (decreased dilution  
> from
> 1:100 to 1:65, new bottle of antibody)
> 30 min incubation at room temp of ImmPRESS anti-Mouse Reagent from  
> Vector
> Develop stain with DAB chromogen from Vector
>
> Washes of PBS/Tween 20 follow between steps
>
> Thank you,
> Jennifer Hill
> Research Assistant
> Kansas State University
> Veterinary Diagnostic Lab
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> --
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 83, Issue 46
> 



--
**

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Pax 8

[McMahon, Loralee A]

Biocare,  They have a prediluted.  Works great. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz 
[raest...@grics.net]
Sent: Monday, November 01, 2010 9:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pax 8

Does anyone know where to purchase Pax 8 antibody for formalin fixed paraffin 
embedded tissue?

Rae Staskiewicz
Methodist Medical Center


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Nashville TN Ht Position

[Hale, Meredith]

 

Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time  position
that offers a competitive salary and  the flexible hours allows you to
put your own personal stamp on the laboratory .  Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mh...@carisls.com

 

 

 

 

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mh...@carisls.com   

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 46

[Feher, Stephen]

http://www.cytopathnet.org/tiki-index.php 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miranda
D. Felton
Sent: Saturday, October 30, 2010 1:43 PM
To: histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 46

Does anyone know of a cytology listserv?

Thank you
Miranda

Sent from my iPod

On Oct 30, 2010, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu
wrote:

> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific 
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>   1. RE: lymph nodes peeling out of OCT (Monfils, Paul)
>   2. PLP fixative (Truscott, Tom)
>   3. Precipitating Silver (Sheila Haas)
>   4. RE: Precipitating Silver (Monfils, Paul)
>   5. RE: PLP fixative (WILLIAM DESALVO)
>   6. IHC Staining Problem (Jennifer Hill)
>   7. Laura Miller is Out of  the Office.
>  (laura.mil...@leica-microsystems.com)
>   8. RE: RE: breast fixation times (Patsy Ruegg)
>   9. cryostat question (histo...@aol.com)  10. Re: IHC Staining 
> Problem (Jennifer MacDonald)
>
>
> --
>
> Message: 1
> Date: Fri, 29 Oct 2010 13:13:44 -0400
> From: "Monfils, Paul" 
> Subject: RE: [Histonet] lymph nodes peeling out of OCT
> To: 
> Message-ID:
>
> <4ebff65383b74d49995298c4976d1d5e075e0...@lsriexch1.lsmaster.lifespan.
> org
> >
>
> Content-Type: text/plain;charset="us-ascii"
>
> I place all tissues in liquid OCT for at least 15 minutes before 
> freezing.  This virtually eliminates the problem of tissue sections 
> separating from the OCT after sectioning.
>
>
>
>
>
> --
>
> Message: 2
> Date: Fri, 29 Oct 2010 10:55:45 -0700
> From: "Truscott, Tom" 
> Subject: [Histonet] PLP fixative
> To: "histonet@lists.utsouthwestern.edu"
>
> Message-ID:
> 
> <44f1d6d7eb8cc84f92859ee5c4e6ecb4011b20f1b...@cvmmbx.vetmed.wsu.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> I am seeking basic information on PLP fixative. What is it? Can I make

> it or buy it? How long does fixation take for lymph node?
> Thanks in advance, Tom Truscott
>
>
> --
>
> Message: 3
> Date: Fri, 29 Oct 2010 10:56:14 -0700 (PDT)
> From: Sheila Haas 
> Subject: [Histonet] Precipitating Silver
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <450265.9811...@web57802.mail.re3.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Has anyone heard of precipitating silver out of a solution by adding 
> table salt?
> I read this somewhere as a means of disposing of silver easily. Any 
> info would be appreciated.
> Thanks,
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
>
>
>
>
> --
>
> Message: 4
> Date: Fri, 29 Oct 2010 14:02:26 -0400
> From: "Monfils, Paul" 
> Subject: RE: [Histonet] Precipitating Silver
> To: 
> Message-ID:
>
> <4ebff65383b74d49995298c4976d1d5e075e0...@lsriexch1.lsmaster.lifespan.
> org
> >
>
> Content-Type: text/plain;charset="iso-8859-1"
>
> Yes, it is easy to do. Since silver chloride is insoluble in water, 
> chloride added to a solution containing ionic silver will cause the 
> white compound to precipitate out immediately.  It will settle out 
> onto the bottom of the container overnight, or can be filtered out.
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- 
> boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
> Sent: Friday, October 29, 2010 1:56 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Precipitating Silver
>
> Has anyone heard of precipitating silver out of a solution by adding 
> table salt?
> I read this somewhere as a means of disposing of silver easily. Any 
> info would be appreciated.
> Thanks,
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc.
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> --
>
> Message: 5
> Date: Fri, 29 Oct 2010 12:52:06 -0600
> From: WILLIAM DESALVO 
> Subject: RE: [Histonet] PLP fixative
> To: , histonet
>
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Follow the links for a receipe.
>
> www.pathology.ufl.edu/~molecular/PLP%20Fixative.doc
>
> http://www.niehs.nih.gov/research/atniehs/labs/lep/p

[Histonet] aluminum gutter guard

[DiCarlo, Margaret]

Hello Histonetters, 

I have been using aluminum gutter guard to wrap my bones when I manually
process them- from increasing percentages of alcohols to xylene and
paraffin.  I am running very low on it and I have not been able to find
it at any hardware stores.  I looked on line and Amazon is out of stock
and doesn't even know if they will still carry it.  Does anyone know
what I can use to replace it?  I appreciate your help.

 

 

Peggy DiCarlo HT (ASCP)

Orthopedic Bone Lab

Buffalo General Hospital

Buffalo, NY  14203

716-859-1293

 



2009 Best Places to Work Winner 
Visit our careers page at www.kaleidahealth.org/careers

CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or 
previous e-mail messages attached to it are confidential and intended solely 
for the use of the individual or entity to whom they are addressed. If you are 
not the intended recipient, or a person responsible for delivering it to the 
intended recipient, you are hereby notified that any further review, 
disclosure, copying, dissemination, distribution, or use of any of the 
information contained in or attached to this e-mail transmission is strictly 
prohibited. If you have received this message in error, please notify the 
sender immediately by e-mail, discard any paper copies, and delete all 
electronic files of the message. If you are unable to contact the sender or you 
are not sure as to whether you are the intended recipient, please e-mail 
ist...@kaleidahealth.org or call (716) 859-. 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Medite Microtomes

[Nails, Felton]

I have had some experience with this company and their embedding station but 
not microtome. The small cold area where you orient the tissue tends to ice 
over. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Fields
Sent: Saturday, October 30, 2010 4:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Medite Microtomes

Hi Netters,

Does anyone out there use the Medite Microtomes?  If so, have you had any 
problems with them?  I purchased 3 of them less than a year ago and I have 
already sent them back to Germany because of advancing (back and
forth) issues.  They are telling me (of course) no one else has had these 
issues and they want to send me a third set of them (the 2nd set are demos that 
are having some of the same issues).  I would appreciate any feed back on their 
microtomes and company.
Thank you in advanceI'm afraid I have made a big mistake in buying these.
Carole

Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com



CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by 
Northside Hospital. It may contain information that is confidential, 
privileged, proprietary, or otherwise legally exempt from disclosure. If you 
are not the intended recipient, you are hereby notified that you are not 
authorized to read, print, retain, copy or disseminate this message, any part 
of it, or any attachments. If you have received this message in error, please 
delete this message and any attachments from your system without reading the 
content and notify the sender immediately of the inadvertent transmission. 
There is no intent on the part of the sender to waive any privilege.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE:
 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
 authorized representative of the intended recipient, you
 are hereby notified that any review, dissemination, or
 copying of this e-mail and its attachments, if any, or
 the information contained herein is prohibited.  If you
 have received this e-mail in error, please immediately
 notify the sender by return e-mail and delete this e-mail
 from your computer system.  Thank you.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Pax 8

[Rae Staskiewicz]

Does anyone know where to purchase Pax 8 antibody for formalin fixed paraffin 
embedded tissue?

Rae Staskiewicz
Methodist Medical Center


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FW: I love this story

[Doughty, Adele]

Good morning sure was getting to talk to you the other day. This past
weekend was the first time I heard dad complain that he did not feel
good, headache and nausea. His hair is getting thinner. Not much else
happening here thank goodness. Love ya, A

 



 


 

 





 

 

 

 

 

 

 

"IT'S WHAT YOU SCATTER

I was at the corner grocery store buying some early potatoes... I
noticed a small boy, delicate of bone and feature, ragged but clean,
hungrily apprising a basket of freshly picked green peas.

I paid for my potatoes but was also drawn to the display of fresh green
peas. I am a pushover for creamed peas and new potatoes.

Pondering the peas, I couldn't help overhearing the conversation between
Mr. Miller (the store owner) and the ragged boy next to me.

'Hello Barry, how are you today?'

'H'lo, Mr. Miller. Fine, thank ya. Jus' admirin' them peas. They sure
look good.' 
'They are good, Barry. How's your Ma?'
'Fine. Gittin' stronger alla' time.'
'Good. Anything I can help you with?'
'No, Sir. Jus' admirin' them peas.'
Would you like to take some home?'  Asked Mr. Miller. 



'No, Sir. Got nuthin' to pay for 'em with.'

'Well, what have you to trade me for some of those peas?'

'All I got's my prize marble here.'



'Is that right? Let me see it' said Miller.



'Here 'tis. She's a dandy.' 



'I can see that. Hmm mmm, only thing is this one is blue and I sort of
go for red. Do you have a red one like this at home?' the store owner
asked.


'Not zackley but almost.'

'Tell you what. Take this sack of peas home with you and next trip this
way let me look at that red marble'. Mr. Miller told the boy.

'Sure will. Thanks Mr. Miller.'



Mrs. Miller, who had been standing nearby, came over to help me. 

With a smile she said, 'There are two other boys like him in our
community, all three are in very poor circumstances. Jim just loves to
bargain with them for peas, apples, tomatoes, or whatever.



When they come back with their red marbles, and they always do, he
decides he doesn't like red after all and he sends them home with a bag
of produce for a green marble or an orange one, when they come on their
next trip to the store.' 



I left the store smiling to myself, impressed with this man. A short
time later I moved to Colorado , but I never forgot the story of this
man, the boys, and their bartering for marbles. 



Several years went by, each more rapid than the previous one. Just
recently I had occasion to visit some old friends in that Idaho
community and while I was there learned that Mr. Miller had died. They
were having his visitation that evening and knowing my friends wanted to
go, I agreed to accompany them. Upon arrival at the mortuary we fell
into line to meet the relatives of the deceased and to offer whatever
words of comfort we could.

Ahead of us in line were three young men. One was in an army uniform and
the other two wore nice haircuts, dark suits and white shirts...all very
professional looking. They approached Mrs. Miller, standing composed and
smiling by her husband's casket. 



Each of the young men hugged her, kissed her on the cheek, spoke briefly
with her and
moved on to the casket. Her misty light blue eyes followed them as, one
by one; each young man stopped briefly and placed his own warm hand over
the cold pale hand in the casket. Each left the mortuary awkwardly,
wiping his eyes. 



Our turn came to meet Mrs. Miller. I told her who I was and reminded her
of the story from those many years ago and what she had told me about
her husband's bartering for marbles. With her eyes glistening, she took
my hand and led me to the casket. 

'Those three young men who just left were the boys I told you about.

They just told me how they appreciated the things Jim 'traded' them.
Now, at last, when Jim could not change his mind about color or
sizethey came to pay their debt.'



'We've never had a great deal of the wealth of this world,' she
confided, 'but right now, Jim would consider himself the richest man in
Idaho ..' 



With loving gentleness she lifted the lifeless fingers of her deceased
husband. Resting underneath were three exquisitely shined red marbles. 

The Moral: 
We will not be remembered by our words, but by our kind deeds. Life is
not measured by the breaths we take, but by the moments that take our
breath. 

Today I wish you a day of ordinary miracles ~ A fresh pot of coffee you
didn't make yourself...

An unexpected phone call from an old friend Green stoplights on your
way to work

The fastest line at the grocery store 

A good sing-along song on the radio... 

Your keys found right where you left them. 

Send this to the people you'll never forget. I just did...

If you don't send it to anyone, it means you are in way too much of a
hurry to even notice the ordinary miracles when they occur. 



IT'S NOT WHAT YOU GATHER, BUT WHAT YOU SCATTER THAT TELLS WHAT KIND OF
LIFE YOU HAVE LIVED! 

 

  

"Lif

Re: [Histonet] cryostat question

[Alan Bright]

Dear Susan,
Our Starlet 2212 Bench Top Cryostat has an option for orientation. There are 
many used for Mohs, as holding a low temperature is not a problem. I can email 
you some user references  if. You want.
Regards: Alan Bright
Bright Instrument Co. Ltd.
England 
Sent from my BlackBerry® wireless device

-Original Message-
From: histo...@aol.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Fri, 29 Oct 2010 22:10:21 
To: 
Subject: [Histonet] cryostat question


Does anyone have 'hands on'  experience performing Mohs using a countertop 
cryostat?  Can the specimen head be manipulated to acccomodate a badly embedded 
block? Does the machine stay cold enough to perform Mohs? Any advice would be 
appreciated.  
My e-mail is histo...@aol.com. Thanks, Susan
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


-- 
BEGIN-ANTISPAM-VOTING-LINKS
--


Teach SpamSniper if this mail (ID 01DoOb8La) is spam:

Spam:
http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8La&m=99b5dbb60f3d&c=s

Not spam:
http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8La&m=99b5dbb60f3d&c=n

Forget vote: 
http://admin.spamsniper.co.uk/canit/b.php?i=01DoOb8La&m=99b5dbb60f3d&c=f
--
END-ANTISPAM-VOTING-LINKS

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet