Re: [Histonet] Charged slides

[Marvin Hanna]

Hi Helen,

Plus is the branding for silanized slides, so they are the same. As  
far as magical, I'm not sure, but the silanization procedure  
covalently bonds the amino groups in APES (3- 
Aminopropyltriethoxysilane, available from Sigma) to the silicate  
ions on the surface of the glass, creating a positive charge on the  
slide.


Polylysine coating also puts a positive charge on the slide, but it  
is just a coating, unlike the chemically reacted covalent bond of the  
silanization procedure.


A positive charge on the surface of the slide allows for strong  
bonding with negatively charged formalin fixed tissue.


John Kiernan wrote a paper many years back on adhesives for slides  
that shows the procedure and chemical reaction in silanizing slides.  
It is available here.

http://publish.uwo.ca/~jkiernan/adhesivs.htm

Kind Regards,

Marvin Hanna


On Jan 6, 2011, at 5:43 PM, Helen Fedor wrote:

Hi, A question has come up regarding the different methods used to  
put a charge on the slide. We recently ordered some plus slides and  
the boxes they are packed in say silanized slides, but they say   
"plus" on the slide . We don't want to use these for our clients if  
they are not going to be getting the same results as the former  
"plus" slides that we were using. I was under the delusion that  
Plus slides somehow are magically charged without any coating  
process taking place.


So does anyone know exactly how a "plus" slide gets its charge? Do  
they all get dipped in APES, or Polylysine?


Thanks for your help.

--
Helen

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[Histonet] Histology Training Specialist - Great Opportunity

[Matt Ward]

Happy New Year Histonet!



Our team at Personify is searching for a histology professional for a
Histology Training position with a world leader based out of the Chicago
area. Please contact me directly to learn more.



Thanks!





Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com



*Histology Training Specialist *


*The Company:*

Our client is a leading developer and producer of innovative high-tech
precision optics systems for the analysis of microstructures.  As one of the
market leaders in each of the fields of Microscopy, Confocal Laser Scanning
Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment.
Comprising nine manufacturing facilities in seven countries, sales and
service companies in 20 countries and an international network of dealers,
the company is represented in over 100 countries.

*The Opportunity:*

The company currently has an opening for a Histology Training Specialist.
All applicants must not be adverse to travel, as this is a position that may
require you to travel when necessary.

Base: Depends on Experience

Other: Full benefits - 401k program/matching

*Primary Responsibilities:*

The primary responsibility of this role will be to provide technical phone
support by answering questions, troubleshooting problems, logging and
closing complaint files and escalating major issues to appropriate company
personnel.  This role will also provide technical training on specified
products in the company's newly constructed state-of-the-art Customer
Support Laboratory.  Training programs are designed for small groups to
ensure maximum customer learning and satisfaction.

Additional Responsibilities:
- Provide product and applications phone support to end-users, field
personnel and dealers for all product lines
- Log all calls into Customer Support Database
- Participate in development of training materials and conduct classes and
labs for customers, employees and others as needed
- Serve as technical liaison to Customer Service/Field Service/Product
Management departments

*Education and Experience Required:*

Ability to interact with various people in a calm and positive fashion and
the ability to effectively communicate information to groups of participants
is required.  Experience with data entry, MS Office programs (PowerPoint,
Lotus Notes, Word) is also required.

HT/HTL/QIHC (ASCP) is helpful but not required.





Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com
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[Histonet] VIP 1000

[Lyn Stadler]

Are you looking for the VIP 1000 User Manual?  If so, I can send a copy

Lyn Stadler
Senior Research Associate - Histopathology
Cleveland Biolabs, Inc.
73 High Street
Buffalo, NY 14203
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Thursday, December 23, 2010 8:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 85, Issue 21
Importance: Low

Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Kathleen Dittrich, where are you? (Settembre, Dana)
   2. Physician signature (Webb, Dorothy L)
   3. RE: Physician signature (Weems, Joyce)
   4. Thomas Crowell is out of the office. (thomas.crow...@novartis.com)
   5. RE: RE: Physician signature (Mike Pence)
   6. RE: RE: Physician signature (Weems, Joyce)
   7. High Paying Job Opportunity - Live anywhere (Alisha Dynan)
   8. Fisher MicroProbe manual staining system (Martha Ward)
   9. RE: RE: Physician signature (Morken, Tim)
  10. Frozen Specimen Handling (John Shelley)
  11. RE: RE: Physician signature (Weems, Joyce)
  12. Symphony cover slipper (Clare Thornton)
  13. VIP 1000 Help (Drew Meyer)
  14. FW: ASCP Action Alert - Physician Signature Requirement   _FYI
  (Liz Chlipala)
  15. RE: Frozen Specimen Handling (sgoe...@mirnarx.com)
  16. Used embedding center and microtome (alineum...@aol.com)
  17. CAP Question regarding procedure manual (Victor Tobias)
  18. RE: CAP Question regarding procedure manual (Weems, Joyce)
  19. RE: CAP Question regarding procedure manual (WILLIAM DESALVO)
  20. RE: CAP Question regarding procedure manual (Jesus Ellin)
  21. Re: CAP Question regarding procedure manual
  (bsulli...@shorememorial.org)


--

Message: 1
Date: Wed, 22 Dec 2010 13:18:22 -0500
From: "Settembre, Dana" 
Subject: [Histonet] Kathleen Dittrich, where are you?
To: "Settembre, Dana" , 'IRENA SREBOTNIK KIRBIS'
, "hfe...@jhmi.edu" ,
"ahut...@dh.org" , "histonet@lists.utsouthwestern.edu"

Message-ID:

Content-Type: text/plain; charset="us-ascii"

Kathleen Dittrich, formerly from Dako.
Where are you?
Dana Settembre



--

Message: 2
Date: Wed, 22 Dec 2010 12:36:43 -0600
From: "Webb, Dorothy L" 
Subject: [Histonet] Physician signature
To: "'histonet@lists.utsouthwestern.edu'"

Message-ID:
<65365f35c0f2ef4d846ec3ca73e49c43ea95058...@hpemx3.healthpartners.int>
Content-Type: text/plain; charset="us-ascii"

According to our manager, the physician signature is for clinical testing only, 
not anatomic pathology.  If anyone knows that this is inaccurate, would love to 
see the written rules!!

Thanks and everyone have the most blessed Holiday!!




  
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HealthPartners Support Center by telephone at (952) 967-6600. You will be 
reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0


--

Message: 3
Date: Wed, 22 Dec 2010 13:38:30 -0500
From: "Weems, Joyce" 
Subject: [Histonet] RE: Physician signature
To: "Webb, Dorothy L" ,
"'histonet@lists.utsouthwestern.edu'"

Message-ID:
<92ad9b20a6c38c4587a9febe3a30e164044b332...@chexcms10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

It specifically mentions AP technical charges in the proposal.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Wednesday, December 22, 2010 13:37
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Physician signature

According to our manager, the physician signature is for clinical testing only, 
not anatomic pathology.  If anyone knows that this is inaccurate, would love to 
see the written rules!!

Thanks and everyone have the most blessed Ho

[Histonet] Feulgen Stain for DNA

[Liz Chlipala]

Hello Everyone

 

I have tried to find this answer on the web and in references but I need
some help.  With respects to the Feulgen stain, how sensitive is it? Can
it detect smaller fragments of DNA? Does it have to be double stranded
or will single stranded stain as well?  Thanks in advance.

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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Re: [Histonet] Lost Blocks

[Patrick Laurie]

Here we take a picture of the blocks before they go on the processor.  We
have a barcoding system that lets me know who embedded what and when.  With
over 140,000 blocks/year we only have had one instance where a block was
lost between grossing and embedding.  Because we have some off-site
pathologists and grossing staff, our lab relies very heavily on tracking
every specimen that comes in, every block that goes to histology and every
stained slide that goes to our pathologists.

Good luck, it can be frustrating when you lose a block.

On Fri, Jan 7, 2011 at 9:45 AM, Paula Wilder  wrote:

>
> HI all,
>
> Does anyone have a procedure outlining checks and balances to assure blocks
> have been placed on the processor, blocks that are missing, either before or
> after embedding, etc.  Any help would be greatly appreciated!
> Thank you,
>
> Paula Wilder
> St. Joseph Medical Center
> Towson, MD 21204
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plau...@cellnetix.com
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[Histonet] ArrayMold System

[Heath, Nancy L.]

Wondering if anyone has used/purchased the ArrayMold "tissue micro array
system"?? Positive and negative feedback would be greatly appreciated :)
 
Nancy Heath, HT(ASCP)
 
March 10, 2011 is Histotechnology Professionals Day
 
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[Histonet] control block swap

[Curt Tague]

I have a few good WT-1 control blocks I'm willing to barter with, any
takers?

 

Curt Tague

CEO, Pathology Arts

 

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[Histonet] Cytology blender

[sgoebel]

Hey all,

At an old job we had this tiny little blender with a kind of diaphragm
top on it to blend sputum cytology specimens with.  It probably only
held about 200cc of fluid at the most.  Does anyone have a clue where I
can find one of these?

Thanks

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

 

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[Histonet] Job Opening in Torrance, CA

[Timothy Jay]

Histotech needed in Torrance, CA for a brand new path lab in a 6 physician GI 
practice. Full autonomy, great working conditions, excellent pay and benefits. 
Must be licensed in the State of California and have all necessary education 
and training to work independently. Send resumes to tja...@yahoo.com. 
 
Thank you. 
 
Tim



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[Histonet] Lost Blocks

[Paula Wilder]

HI all,
 
Does anyone have a procedure outlining checks and balances to assure blocks 
have been placed on the processor, blocks that are missing, either before or 
after embedding, etc.  Any help would be greatly appreciated!
Thank you,
 
Paula Wilder
St. Joseph Medical Center
Towson, MD 21204  
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[Histonet] Toluidine blue staining of CFU-F

[Adam .]

Hi all,

I'm looking for a protocol to counterstain mouse CFU-F grown in tissue
culture**. The protocols I've found just say they counterstained with this
dye, don't say what it's dissolved in, sometimes list the percentage of it,
and never list the time they stained for. The only full protocol I found was
for staining of mast cells in sections, but that's dissolved in 70% ethanol
and very acidified. I'm not sure that's what I want.

Has anyone done this before?

Thanks,
Adam
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[Histonet] re: Pax-2

[Connelly, John]

We have been using the invitrogen AB.  It does not have much background and 
works well.



John Connelly, M.D.



"Message: 21

Date: Tue, 4 Jan 2011 16:06:56 -0800

From: Mark Tarango mailto:marktara...@gmail.com>>

Subject: [Histonet] Pax-2

To: 
"histonet@lists.utsouthwestern.edu"


mailto:Histonet@lists.utsouthwestern.edu>>

Message-ID:


mailto:aanlktimha5h26-8o6fx4wuk5oplobrog4nexmpsnt...@mail.gmail.com>>

Content-Type: text/plain; charset=ISO-8859-1



Hi Histonet,



My lab needs to switch vendors on this antibody.  Where is everyone buying 
Pax-2?  I found on their website that Invitrogen sells an IVD rabbit poly

Pax-2 antibody.  Has anyone tried this or others?  What about Epitomics RUO 
rabbit mono?  Do all the antibodies give that cytoplasmic background staining 
that I've seen?



I'd appreciate any info at all.



Thanks



Mark Tarango"


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[Histonet] Great histology jobs in Maine

[Alisha Dynan]

Hi Histonet Members,

 




I am a healthcare recruiter, specifically focused on the laboratory/pathology 
space. I have openings all across the country and would be interested in 
speaking with anyone interested in searching for a new job. Below is a great 
new opportunity that I am working on:

 

Histotech and Histology Supervisor Opening in Maine:

I am currently working on an amazing opportunity with one of largest most 
sophisticated labs in the Northeast. It is located in coastal Maine. This 
company is looking to hire on a Histology Supervisor  and a Histotech.  They 
currently are looking for someone with a Bachelor's Degree, histology 
experience, HTL(ASCP) or HT(ASCP) certified, and supervisory experience for the 
supervisor position. The compensation package is fantastic and includes health, 
dental, a retirement plan, and relocation assistance, if needed. If interested 
in learning more details, please email me at ali...@ka-recruiting.com.

 

If you are not interested but know of someone you could recommend for this 
position, please pass on their contact information to me. We have a referral 
bonus for anyone you refer to me that I place into a new job.

 

I am also working on the following histology/pathology jobs:

* Technical Support Specialist - Western territory (need PA or HTL license)  



* Technical Support Specialist - PA/NY territory (need PA or HTL license)
* Technical Support Specialist - Southeastern territory (need PA or HTL license)


* MI - Dermpath Histology Manager

* OK - Histology Supervisor

* NV - Histotechnologist 3rd shift

* NY - Long Island - Dermpath Lab Manager

* NH - Histology Supervisor

* Eastern OH - Histology Supervisor

* Maine - Histotechnologist

* Maine - Histology Supervisor

* FL - Histotechnologist

* NY City - Histology Supervisor with IHC experience

* NY City - Histotechnologist

* NY City - Grossing Tech

* NV - Pathologist's Assistant


If interested in any of the opportunities above, please email me.







Sincerely,

 

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

ali...@ka-recruiting.com

www.ka-recruiting.com

 






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[Histonet] isosulfan blue for rodent lymph node dissection

[Connolly, Brett M]

Happy New Year all - 

Has anyone had experience with injecting isosulfan-blue sub-q into
rats/mice priori to euthanizing for coloring of lymph nodes to make them
easier to find ??

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




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proprietary copyrighted and/or legally privileged. It is intended solely
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Re: [Histonet] Stupid, stupid static!!

[Jay Lundgren]

They use static guard spray here at this facility in IL, but it does make it
smell like  your matronly aunt just walked into the room.

 Jay A. Lundgren M.S., HTL
(ASCP)





> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

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Re: [Histonet] Stupid, stupid static!!

[histot...@imagesbyhopper.com]

I have a tech who complains about static/compression in our cell blocks, but I 
don't seem to have the same problem as her.  The difference? I face my blocks 
and then soak it on my waterbath for about 15 -30 seconds and then it goes onto 
the ice tray.  The additional moisture that is soaked up in the block seems to 
correct the issue for me.  Sort of like breathing heavily on the block!

Michelle


On Jan 6, 2011, at 4:36 PM, "Johnson, Nacaela"  
wrote:

> I am also having issues with static, but only on my particle blocks.
> This happens in the summer as well.  The static is actually "in" the
> block.  The core biopsies cut wonderfully.  Does anyone else run into
> this problem?  I can see the effects of the static when I'm embedding,
> the particles float to the edges of the mold instead of in a clump. 
> 
> 
> Thanks,
> 
> Nacaela Johnson
> Histology Technician
> KCCC Pathology
> 12000 110th St., Ste. 400
> Overland Park, KS 66210
> Office:  913-234-0576
> Fax:  913-433-7639
> Email:  nacaela.john...@usoncology.com
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> sgoe...@mirnarx.com
> Sent: Wednesday, January 05, 2011 1:15 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Stupid, stupid static!!
> 
> So where the microtome is here that I have to use we have to wear those
> blue hospital booties and disposable lab coats (the white paper type
> ones).  With me and several other people walking around in those booties
> the amount of static electricity is to say the least frusterating!!
> Does anyone know of anything I can do to get rid of the static?
> 
> Thanks
> 
> 
> 
> Sarah Goebel, BA, HT(ASCP)
> 
> Histotechnologist
> 
> Mirna Therapeutics
> 
> 2150 Woodward Street
> 
> Suite 100
> 
> Austin, Texas  78744
> 
> (512)901-0900 ext. 6912
> 
> 
> 
> 
> 
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> 
> 
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RE: [Histonet] RE: Static issues

[Margaret Blount]

I live in moist old UK and have always breathed on my ribbons, can't get
out of the habit, but do remember to breath and not too heavily!

On the cryostat front, I used to suffer from static terribly and tried
all sorts of things, but since using disposable blades have had fewer
problems. I just move the blade along or replace it and it often cures
the problem. 

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: 06 January 2011 21:50
To: Breeden, Sara; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Static issues

I was going to say the same thing... Just hadn't taken time. So may of
us are full of hot air.. J:>) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Thursday, January 06, 2011 16:45
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Static issues

This is New Mexico where "humidity" is a rumor.  The humidity in the lab
here - as I write - is 18% and that's on a really wet day!  If I have
static issues with my ribbons, I just lean a little bit toward the block
and "breathe" on it and the ribbons just float (in a good way) off the
knife.  I do that so often that when I use my sewing machine, I find
myself breathing on the material.  That's just sad!  But try the
Breathing Thing.  Or not.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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