[Histonet] dual fluorescent staining
Hello everyone, I am working on a protocol for a dual fluorescent stain with two mouse antibodies (one is IgG1 and the other is IgG2a). We started by performing the two stains sequentially with a serum free protein blocking step in between, tagging one antibody with a 488 FITC labeled secondary and a 568 Cy3 secondary on the other (both goat anti mouse of course). With this method we are getting a lot of background staining so I was wondering if anyone who has experience in this sort of work if you have a recommendation of a proper blocking solution between the sequential stains? I've heard about using Rodent Block Rat from Biocare Medical or an un-conjugated goat anti mouse IgG secondary immunoglobulin but I would like to hear from you all as well! :-) Thanks, Ross ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Looking for a product
Was wandering of any of you guys have used either a anti MCP or IL1beta on rat brain. Thanks y'all ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TGFβ RII
Hi histonetters, I need suggestion and help with the TGFβ RII (D-2): sc-17799 Ab, which should show a cytoplasmic staining. My problem is that I am getting a beautiful nuclear staining only. How I can fix this problem? What to pay attention on and what to change in the usual protocol? My AR is citrate buffer pH6. This is a monoclonal mouse Ab against human TGFβ RII. This is only Ab that can work for me because it recognize only human TGFβ RII and does not cross react with mouse. Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I just sort of fell into Histology some 40 plus years ago and never looked back. I have left the field and always come back to it. I have done research and clinical and still love it. Pam Marcum UAMS Sent from my Verizon Wireless BlackBerry -Original Message- From: bsulli...@shorememorial.org Sender: histonet-boun...@lists.utsouthwestern.edu Date: Wed, 26 Jan 2011 15:50:17 To: Cc: ; Subject: RE: [Histonet] (no subject) Back in the day when I was trying to decide which school to pick for my scholarship monies, I had a choice of the Med Tech program, the Cytology program or the Histotechnology program. I had absolutely no idea what Histology was but that is the program I choose. One of the best decisions I ever made. I have never, ever, ever been bored with this field. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Sent by: To histonet-bounces@ , lists.utsouthwest ern.educc Subject 01/26/2011 03:42 RE: [Histonet] (no subject) PM Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more fun!! I would say maybe try and go visit a lab that has both types of techs there and see what looks like it would fit you best. Good luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 26, 2011 1:47 PM To: Histonet Subject: [Histonet] (no subject) Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks ORO
Leave it to histonet to provide numerous responses! Thank you all for providing me with your formula's and techniques. I have some great information! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Back in the day when I was trying to decide which school to pick for my scholarship monies, I had a choice of the Med Tech program, the Cytology program or the Histotechnology program. I had absolutely no idea what Histology was but that is the program I choose. One of the best decisions I ever made. I have never, ever, ever been bored with this field. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Sent by: To histonet-bounces@ , lists.utsouthwest ern.educc Subject 01/26/2011 03:42 RE: [Histonet] (no subject) PM Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more fun!! I would say maybe try and go visit a lab that has both types of techs there and see what looks like it would fit you best. Good luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 26, 2011 1:47 PM To: Histonet Subject: [Histonet] (no subject) Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more fun!! I would say maybe try and go visit a lab that has both types of techs there and see what looks like it would fit you best. Good luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 26, 2011 1:47 PM To: Histonet Subject: [Histonet] (no subject) Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] glass cleaner
HISTOLOGY UNIVERSAL WASHING INSTRUCTIONS FOR GLASSWARE Alternative to Acid Cleaning Glassware 5% Clorox is a very inexpensive and readily available chemical treatment solution, which is an alternative to acid cleaning. All glassware used for special stains MUST be cleaned in the following manner. This method MUST be used when using glassware for special stains sensitive to metal, such as FE, GMS, Retic, Jones, Dieterle, Steiner and Warthin-Starry. 1.Make fresh each morning a 5% Clorox solution containing 1/2 Tbsp. per 5 gallons. of Liqui-Nox. 2.Soak all glassware in this solution for a minimum of 30 minutes to remove any stains, and then scrub with brush until all residue is removed. 3.For hard to get out stains, soak in a stronger solution of Clorox. 4.Rinse with copious amounts of tap water, followed by several changes of deionized water. All traces of Clorox must be removed. 5.Place glassware on drying rack or upside down to dry thoroughly. 6.Use caution that NO metal instruments touch glassware. This will contaminate certain solutions, which must be free of any metal, such as Iron and Silver Stains. 7.After glassware is dry, put parafilm or gauze around top so no airborne contaminate may enter. 8.Put glassware away. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Jan 26, 2011, at 11:42 AM, Johnson, Nacaela wrote: Hello I am looking for suggestions on the best chemical cleaner for glassware that is used for special stains. Any ideas? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Yes, I just started working in a histology lab. Thats y I was asking this question. I heard about cytology and wanted to get some opinions as to who offered what! Just trying to keep the options open. I doubt you are on any blacklist. LOL!! I remain yours truely, Candice Camille From: V. Neubert To: histonet@lists.utsouthwestern.edu Sent: Wed, January 26, 2011 1:54:27 PM Subject: Re: [Histonet] (no subject) Is there a chance to go and see a lab which does histology? > Hello > > I am really new to the field of histlogy. I wanted to get some opinions on >this > > field vs. cytology. I am wondering which field is better for me. Any >suggestions > > or thoughts are welcome. Thanks!!! > > I remain yours truely, > > Candice Camille > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Is there a chance to go and see a lab which does histology? > Hello > > I am really new to the field of histlogy. I wanted to get some opinions on > this > field vs. cytology. I am wondering which field is better for me. Any > suggestions > or thoughts are welcome. Thanks!!! > > I remain yours truely, > > Candice Camille > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Go for HISTOLOGY!! Cytology requires a lot of Sitting in One Place Staring into a Microscope (please! - no offense to cytotechs - really!). With histology, your daily routine is varied and invigorating. And now I'm probably on some Cytology Tech Blacklist... sigh... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] glass cleaner
~ 90 mL 70% EtOH ~ 10 mL 25% HCl Removed everything I ever had to deal with. After that give your glassware a good wash in the dishwasher, then rinse with plenty of demineralized water. And wear protective gloves, but as we all do, I actually don't have to remind any of the list members, right? ;) > Hello > > I am looking for suggestions on the best chemical cleaner for glassware > that is used for special stains. Any ideas? > > Thanks, > > Nacaela Johnson > Histology Technician > KCCC Pathology > 12000 110th St., Ste. 400 > Overland Park, KS 66210 > Office: 913-234-0576 > Fax: 913-433-7639 > Email: nacaela.john...@usoncology.com > > The contents of this electronic mail message and any attachments are > confidential, possibly privileged and intended for the addressee(s) > only.Only the addressee(s) may read, disseminate, retain or otherwise use > this message. If received in error, please immediately inform the sender and > then delete this message without disclosing its contents to anyone. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] glass cleaner
Hello I am looking for suggestions on the best chemical cleaner for glassware that is used for special stains. Any ideas? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Needed in Santa Rosa, CA.
Certified Histotech needed in Santa Rosa, CA. Part-time (25-30 hrs/wk) opportunity for an experienced and certified tech to work in a brand new GI path lab. Competitive pay and wonderful working environment. Send resumes to tja...@yahoo.com. Kind Regards, Timothy Jay Pillar Consulting, LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil Red O for FS on Muscle
I've done many of these stains and I get my ORO stain from Poly Scientific. Actually I use the whole kit and I use Freida's protocol in the second edition. That includes fixing in 37% formaldehyde. I filter the ORO just before use and usually get nice clean slides. If there is a residue it isn't much and wipes off easily. I even re-use the ORO stain once in a while and it still comes out great. My slides are often used for quantifying the amount of lipids so I don't counterstain most of the time. Andi Grantham On Jan 26, 2011, at 10:15 AM, Akemi Allison wrote: > Happy hump day! > > Does anyone have a good procedure for Oil Red O for FS on Muscle. > The procedure the lab I am working with is having a great deal of > problems with their muscle biopsy panel. I am trouble-shooting some > of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am > working with them with pH issues. > > The Oil Red O is very problematic. They are mixing before use and > filtering with 42 Watman paper, but there is a lot of residual > background on the slides. Also, they were not fixing the sections > with 37% formaldehyde, even though the procedure calls for it. Could > you share who you get the stain from also. Your assistance is > greatly appreciated. > > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3...@yahoo.com > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica autostainer XL
I have a run set up to bake the slides & run them down to water ( for my specials), another just to start at hematoxylin down to xylene etc. The stainer allows for nearly any custom program you can think of. :o) If you have any questions about how to set the program up, please let me know & I will be happy to help you with it. Michelle On Jan 26, 2011, at 11:52 AM, "Langenberg, Stacey" wrote: > You can set up a just depar run. > > Sent from myTouch 4G > > - Reply message - > From: "Nicole Tatum" > To: "histonet@lists.utsouthwestern.edu" > Subject: [Histonet] Leica autostainer XL > Date: Wed, Jan 26, 2011 9:48 am > > > > Does anyone know if you can just deparaffinize slides in the stainer. > > Im just wondering if I also need a small stain line to dewax specials. > > Thank you, > Nicole > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oil Red O for FS on Muscle
Allison We make up our Oil Red O from scratch same day we use it, let it stand 10 minutes, and then filter with a Millipore Stericup 0.22µm, GP Express Plus Membrane, 250ml Receiver Bottle, catalog number: SCGPU02RE under vacuum. That seems to help a bit with the background and we are working with a really clear solution. We have not run muscles but aortic root and liver on mouse and rat. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 26, 2011 10:16 AM To: histonet Subject: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oil Red O for FS on Muscle
Hi, We use Oil Red O - but we don't do it on muscle. Our protocol is we filter the whole bottle before we use it and then we apply it to unfixed slides for about 30 minutes. Rinse in tap water, counterstain with Hematoxylin and coverslip with Aquamount. We use it for cytospins and occasionally on various frozen tissues. Our stain is from American Mastertech. Hope this helps, Lisa V. Children's Memorial Hospital Chicago, IL 60616 773-868-8949 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 26, 2011 11:16 AM To: histonet Subject: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O for FS on Muscle
Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FX111a Protocol
Hi, We run FactorX111a clone AC-1A1 on the Ultra, here is our protocol: 1. De-paraffinazation (selected) 2. Cell conditioning #1 20 minutes, then 36 minutes. 3. Antibody for 24 minutes 4. Ultra wash (selected) 5. Hematoxylin 4 minutes 6. Bluing 4 minutes. Hope this helps. Lisa V. Children's Memorial Hospital Chicago, IL 60616 773-868-8949 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Wednesday, January 26, 2011 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FX111a Protocol Does anyone have a current protocol for Factor X111a on the Ventana Ultra that is working for you? I would appreciate any info or support you could give. We are using the AC-1A1 clone from Cell Marque for derms. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica autostainer XL
You can set up a just depar run. Sent from myTouch 4G - Reply message - From: "Nicole Tatum" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Leica autostainer XL Date: Wed, Jan 26, 2011 9:48 am Does anyone know if you can just deparaffinize slides in the stainer. Im just wondering if I also need a small stain line to dewax specials. Thank you, Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica autostainer XL
Does anyone know if you can just deparaffinize slides in the stainer. Im just wondering if I also need a small stain line to dewax specials. Thank you, Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] used Leica XL
I've been asked to get pricing for possible purchase of a good used/refurbished Leica autostainer XL. Gale Limron CT, HT (ASCP) Histology Supervisor This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sunquest CoPath Rep
Could any Sunquest CoPath sales rep please contact me privately off the list? Thanks, Drew Meyer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FX111a Protocol
Does anyone have a current protocol for Factor X111a on the Ventana Ultra that is working for you? I would appreciate any info or support you could give. We are using the AC-1A1 clone from Cell Marque for derms. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
