RE: [Histonet] expired abs

[Ingles Claire]

I think my "abs" expired long ago!
Happy Friday
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Patsy Ruegg
Sent: Thu 3/10/2011 8:28 PM
To: 'ihcrg Group (E-mail)'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] expired abs



Just wanted to remind everyone if you are about to discard expired abs I
would be willing to take them and put them to good use. 



If you have expired abs to get rid of contact me and I will provide you a
fedex acct. # so you can send them to me rather than discard.  This effort
paid off recently because I sent a care package of expired abs and detection
reagents to a hospital tech in Ghana West Africa who is using them to test
patients there because they have training in ihc but do not have the abs or
detection reagents to do it.



Regards,

Patsy





Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net

www.ihcrg.org



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[Histonet] Invitation to connect on LinkedIn

[Joyce Bidwell via LinkedIn]

LinkedIn
Joyce Bidwell requested to add you as a connection on LinkedIn:
--

Jackie,

I'd like to add you to my professional network on LinkedIn.

- Joyce

Accept invitation from Joyce Bidwell
http://www.linkedin.com/e/yvpgd1-gl4lbn2r-65/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1187336310_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnP0NcPoPcPsUcj59bRhCul5ehnkRbPkMdzgQcPgUd38LrCBxbOYWrSlI/EML_comm_afe/

View invitation from Joyce Bidwell
http://www.linkedin.com/e/yvpgd1-gl4lbn2r-65/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1187336310_3/3dvc34PdzcPdPwNckALqnpPbOYWrSlI/svi/


 
-- 
(c) 2011, LinkedIn Corporation
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[Histonet] expired abs

[Patsy Ruegg]

Just wanted to remind everyone if you are about to discard expired abs I
would be willing to take them and put them to good use.  

 

If you have expired abs to get rid of contact me and I will provide you a
fedex acct. # so you can send them to me rather than discard.  This effort
paid off recently because I sent a care package of expired abs and detection
reagents to a hospital tech in Ghana West Africa who is using them to test
patients there because they have training in ihc but do not have the abs or
detection reagents to do it.

 

Regards,

Patsy

 

 

Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net 

www.ihcrg.org

 

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[Histonet] Histology Manager Position in Augusta, GA.

[Oelrich, David]

Hello everyone,

Just a note to let you all know we have an opening in our Augusta, GA lab for a 
Histology Manager.

The position is a combination bench/manager position supervising 5-6 techs in a 
reference/specialty Urology AP lab. Great facility, great team, great city.

For more info or to apply: 
http://tbe.taleo.net/NA6/ats/careers/requisition.jsp?org=ENDO&cws=8&rid=2594 
and www.healthtronics.com/lab

Relocation funds available for good candidates. Excellent benefits and 
competitive pay.

Happy cutting!
Dave Oelrich
HealthTronics
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Re: [Histonet] Control Slides

[Lee & Peggy Wenk]

I've noticed that our spirochete control slides don't stain as intense 
starting about 3 months after they are sectioned, and that they stop 
staining by about 6 months. (This is with a silver stain.)


I've talked with other histotechs, and they say they've seen the same 
phenomenon with AFB and Gram controls. (We use ours up too quickly - we 
never have 6-12 months old control slides for these, so I can't attest to 
this. )


Bancroft's book talks about this phenomenon with amyloid, and suggests that 
oxidation of the tissue (proteins) due to exposure to air may be the cause. 
I'm guessing the it probably applies to the precut microorganism control 
slides, too.


We only cut enough slides for 6 months, and date them. Other people say they 
put their cut control slides in a slide box with a lid after drying, and 
then place the box in the refrig, as cold slows down the chemical change, 
and the lid keeps the moving air off the slide. Other people say that, after 
they dry the slide, they dip the slide in paraffin, to cover the tissue, so 
that the air doesn't touch the tissue during the months before the slide is 
used in a stain. Just 3 suggestions to stop this problem.


Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

--
From: "Amador, Amanda" 
Sent: Wednesday, March 09, 2011 10:22 PM
To: 
Subject: [Histonet] Control Slides

Is there guidelines for how long special stains controls are good for once 
they are cut?  We have spirochetes for our Steiner that is from 2007 and 
we are having issues.


Amanda Amador, HT(ASCP)CM
AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | 
Indianapolis, IN  46219 | phone 317.275.8052 | 
aama...@ameripath.com | 
www.AmeriPath.com


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RE: [Histonet] Control Slides

[Debra Siena]

One of the things that I usually suggest is that when every time that you cut 
the control, sequentially number the slides and test the first section and the 
last to make sure that you have not cut through the area of interest and keep 
that slide as part of the quality assurance/quality control.  If both sections 
fit, you could even put on the same slide and mark which is which for cost and 
space savings.  

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
Direct: 972-436-1010  x229 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: Thursday, March 10, 2011 4:11 PM
To: 'Sheila Fonner'; 'Amador, Amanda'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Control Slides

Sheila,
I believe that Amanda is referring to control slides cut for the Steiner 
Modification of the Warthin-Starry stain not for immunohistochemistry.
I would be surprised if the control slides for the Steiner's would "go off". It 
is possible that the technique has gone out of control.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, 10 March 2011 11:53 PM
To: 'Amador, Amanda'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Control Slides

Amanda,

You will lose antigenicity on the spirochete control slides after that long of 
a period.  We usually cut no more than 10 in advance, and we keep them in the 
refrigerator.  Hope this helps.

Sheila HT (ASCP)
Knoxville Dermatopathology Lab
Knoxville, TN


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amador, Amanda
Sent: Wednesday, March 09, 2011 10:22 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Control Slides

Is there guidelines for how long special stains controls are good for once they 
are cut?  We have spirochetes for our Steiner that is from 2007 and we are 
having issues.

Amanda Amador, HT(ASCP)CM
AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | 
Indianapolis, IN  46219 | phone 317.275.8052 | 
aama...@ameripath.com | 
www.AmeriPath.com

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you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
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This note also confirms that this email message has been virus scanned and 
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RE: [Histonet] Control Slides

[Tony Henwood]

Sheila,
I believe that Amanda is referring to control slides cut for the Steiner 
Modification of the Warthin-Starry stain not for immunohistochemistry.
I would be surprised if the control slides for the Steiner's would "go off". It 
is possible that the technique has gone out of control.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, 10 March 2011 11:53 PM
To: 'Amador, Amanda'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Control Slides

Amanda,

You will lose antigenicity on the spirochete control slides after that long of 
a period.  We usually cut no more than 10 in advance, and we keep them in the 
refrigerator.  Hope this helps.

Sheila HT (ASCP)
Knoxville Dermatopathology Lab
Knoxville, TN


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amador, Amanda
Sent: Wednesday, March 09, 2011 10:22 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Control Slides

Is there guidelines for how long special stains controls are good for once they 
are cut?  We have spirochetes for our Steiner that is from 2007 and we are 
having issues.

Amanda Amador, HT(ASCP)CM
AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | 
Indianapolis, IN  46219 | phone 317.275.8052 | 
aama...@ameripath.com | 
www.AmeriPath.com

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you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
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This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at Westmead 
accepts no liability for any consequential damage resulting from email 
containing computer viruses.
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[Histonet] AUTO: Carolyn J Kent is out of the office. (returning 03/11/2011)

[CKent]

I am out of the office until 03/11/2011.

I will respond to your message when I return.


Note: This is an automated response to your message  "Histonet Digest, Vol
88, Issue 12" sent on 3/10/2011 12:05:55 PM.

This is the only notification you will receive while this person is away.


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[Histonet] Ohio State Clinical Histology Manager Position

[Whitaker, Bonnie]

Hi Everyone,

We are still searching for the right person to fill the Clinical Histology 
Manager position at The Ohio State University Medical Center in Columbus, Ohio.

The lab does a brisk volume, our daily block count is around 760.  We are open 
from Sunday evening until Saturday morning, with call rotation for Saturday 
morning through Sunday evening.  It is a great group of people to work with.

The official blurb is:

The OSUMC clinical histology laboratory is a functional laboratory within the 
Anatomic Pathology branch serving the pathology subspecialty divisions; 
Histology Lab Manager will provide leadership for daily operations of the 
histology labs; manages human resources, financial and budget planning and 
preparation, new test development, quality improvement, safety programs, 
compliance program, successful inspection and accreditation processes, and 
ensuring current policies and procedures related to technical, operational, and 
administrative areas of the laboratory are adhered to.

We prefer someone with a solid background in clinical histology, as well as 
supervisory experience.

If you are interested, please send me an email with your current resume/CV.

Thanks!
Bonnie

Bonnie Whitaker

AP Operations Director
The Ohio State University Medical Center
Anatomic Pathology Branch
Department of Pathology
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

bonnie.whita...@osumc.edu

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013


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[Histonet] Thomas Crowell is out of the office.

[thomas . crowell]

I will be out of the office starting  03/10/2011 and will not return until
03/14/2011.

Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
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[Histonet] Job Post for NY

[Eric Sulkosky]

New Urology Histology lab opening in Booklyn, NY. Hiring full and partime
histology techs. If interested please send cv and letters of interests to

Ellen Barbarino
ebarbar...@siradonc.com

Thanks and have a great day!
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[Histonet] It's March 10th. . .

[David Kemler]

Hi HistoNetters -

Have an absolutely, marvelous, fantastic and needless to say, HAPPY 
Histotechnology Professionals Day! I've been plugging it on my show since 
January 30th, so I hope everyone is celebrating "OUR" day!

Regards,
David
www.HistoTALK.com



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[Histonet] celloidin tissue staining

[Crystal Verdick]

I am currently working with 30-40 year old celloidin embedded tissue
that I have sectioned myself. I have used a myelin stain of a modified
Weigert's and am trying to get a Nissl stain to work with no luck.

The 40 micron sections are being kept in 70% etoh in individual series
jars. Weigert's stain works perfectly however when I attempt to stain
for Nissl substance the sections turn out blotchy, uneven and faded as
if the background is the only thing that is holding the stain. I have
tried soaking the sections in 50% etoh for at least an hour before
going into dH20 for 2 hours up to 2 days. Nothing has worked. Does
anyone have any ideas that might help? Something to dissolve a
possible mordant? My only other option is to dissolve the celloidin in
a 50/50 mixture of absolute etoh and ether which could damage the
tissue/structures within.

Crystal V.
Saint Louis University
Center for Anatomical Science Education

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[Histonet] TTC staining & Happy Histotechnology Professionals Day

[John Shelley]

I hope all of you are being appreciated today and really every day for the hard 
work that you do each and every  day to make a difference in people's lives.

Now down to some business, I was hoping to talk with someone either on line or 
off line about TTC staining on infracted hearts and how to perform this 
technique. Whether it is better to perfuse or to slice the hearts and then 
stain. I really need someone to mentor me through the whole process. Thanks in 
advance for whatever help you may be able to give me.

Kind Regards!

John J Shelley


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[Histonet] BOND Max vs. BOND III

[Nancy Schmitt]

Happy Histotechnology Professional Day!

We currently have a Bond Max and are happy with it - looking for users of the 
Bond III to see if you like it - is it really almost maintenance free? - is it 
really faster?  Honest opinions appreciated!

Thanks
Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
Dubuque, IA



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[Histonet] Happy National Histotechnology Day from Pam Barker at RELIA Solutions

[Pam Barker]

Hi Histonetters!

How are you?  I wanted to take a moment and say Happy Histotechnology
Professionals Day.  

I want to thank you not only for the job that you do and the lives that
you save but also for allowing me to work with you in improving your
careers.  In all my years of recruiting I have never worked with a more
professional, appreciative, honest and truly enjoyable group of people.
Kudos to you!!

 

I am curious as to how everyone has celebrated today.  I starting
celebrating yesterday by contacting each and every one of my 1000+
client/employers to let them know that today is  National
Histotechnology Professionals Day.  Hopefully, if they weren't already
doing something to show their appreciation they are now!!  I hope
everyone has a great day!!

I do have some BRAND NEW positions that I would like to tell you about
as well.

Here is a list of my current openings.  All of these positions are
fulltime permanent positions and my clients offer excellent salaries,
benefits and relocation assistance.

 

HISTOLOGY/PATHOLOGY  MANAGEMENT
ME – Histology Supervisor – Scarborough, ME

CA - Histology Lab Manager – Central Valley of CA

CO – Histology Manager – Colorado Springs

LA - Histology Supervisor – Baton Rouge, LA

WI – IHC Specialist/Supervisor -Milwaukee

 

HISTOTECHS

FL – Fort Myers – Dermpath Histotech

FL – Panama City Fl. Lic histotechnologist

MA – North of Boston dermpath exper a plus.

NC – Charlotte Mohs Tech

NC – Charlotte Dermpath Histotech

PA – Southern PA Dermpath Histotech

ME – Scarborough Histology Tech. 

IL – Chicago Histology Training Specialist

WI – Lead Histotech/Immunohistochemistry Specialist

OR – Portland Histotech

 

PATHOLOGY/PATHOLOGIST'S ASSISTANTS

NC – Charlotte PA grad from NAACLES program required
NY – Lead Medical Technologist – Generalist/Days

MA – Medical Technologist - Generalist

 

If you or anyone you know might be interested in any of these positions
or want help with a job search in another area please contact me.  I can
be reached at 866-607-3542 or rel...@earthlink.net.

 

Happy Histo Day!!  Pam



Thank You!

 


Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net

www.myspace.com/pamatrelia

www.twitter.com/pamatrelia 

 

 

 

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[Histonet] Happy Histo Day

[Phyllis Thaxton]

Happy Histotechnology Day EveryoneI want to pass this on from Sharon 
Wehman of Sakura Finetek. Thank you Sharon!

It gives me great pleasure to celebrate this day and give thanks to the 
wonderful histotechs!
 
 
-Thank you for dedicating your expertise and knowledge to every specimen, 
block, 
slide and stain
 
-Thank you for being a part of and making a difference in every diagnosis
 
-Thank you for working hard every day and going above and beyond on most days
 
-Thank you for the incredible care and consideration you give to every patient, 
even though most do not realize what you do--that is EXCELLENCE!
 
 
Excellence is never an accident; it is the result of high intention, sincere 
effort, intelligent direction, skillful execution and the vision to see 
obstacles as opportunities. 

 
 
May Your Histotechnology Day Be As Wonderful As You Are!

 
Sharon Wehman 
Area Manager 
Sakura Finetek USA, Inc. 
1750 West 214th Street 
Torrance, CA 90501 
Office:800-725-8723 X:7274 
Cell:678-462-6349 
sweh...@sakuraus.com 
www.sakuraus.com
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 



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RE: [Histonet] diastase

[Bonner, Janet]

Porcine amylase has worked for us (of course, so has the spit!)

Janet 


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com 
[sgoe...@mirnarx.com]
Sent: Wednesday, March 09, 2011 9:56 AM
To: dmcc...@ckha.on.ca; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] diastase

Just hack up some spit...

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana
McCaig
Sent: Wednesday, March 09, 2011 8:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] diastase

I am looking to purchase diastase to use for the PAS/DIASTASE stain.  I
see there are many options in the catalogues.  What is recommended?



diana

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[Histonet] Histologist Position at Tufts University Boston

[Papalegis, Derek]

Histotechnologist Position at Tufts University, Boston

Tufts University Division of Laboratory Animal Medicine (DLAM) in Boston, MA, 
has an immediate opening for a histotechnologist to provide research and 
diagnostic histology and immunohistochemistry support for Tufts University and 
Tufts Medical Center researchers.  The histotechnologist will manage day-to-day 
operations of the Animal Histology Core under the guidance of the veterinary 
pathologist in the Research Animal Health and Pathology Support Laboratory and 
will work closely with investigators to meet their research histology needs.  
The histotechnologist is expected to function independently to manage technical 
operations of the laboratory with minimal supervision from the pathologist. 
Responsibilities include specimen processing; cryostat sectioning; slide 
production; routine, histochemical and immunohistochemical (IHC) staining; IHC 
protocol development; meeting quality control standards; assisting the 
pathologist as needed; training investigative staff; and development of new 
services for the laboratory.  Applicants must be eligible or certified as 
American Society of Clinical Pathology (ASCP) Histotechnician (HT) or 
Histotechnologist (HTL) and have an Associate's Degree or higher. At least 3-5 
years experience, including experience in immunohistochemistry and experience 
with animal tissues, is preferred.  

For further information or to apply: 
https://recruiter.kenexa.com/tufts/cc/CCJobDetailAction.ss?command=CCViewDetail&job_REQUISITION_NUMBER=56133&ccid=bupJEdUjsTs%3D

Please use the link above if you require more information or would like to 
apply.



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RE: [Histonet] Control Slides

[Sheila Fonner]

Amanda,

You will lose antigenicity on the spirochete control slides after that long
of a period.  We usually cut no more than 10 in advance, and we keep them in
the refrigerator.  Hope this helps.

Sheila HT (ASCP)
Knoxville Dermatopathology Lab
Knoxville, TN


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amador,
Amanda
Sent: Wednesday, March 09, 2011 10:22 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Control Slides

Is there guidelines for how long special stains controls are good for once
they are cut?  We have spirochetes for our Steiner that is from 2007 and we
are having issues.

Amanda Amador, HT(ASCP)CM
AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A |
Indianapolis, IN  46219 | phone 317.275.8052 |
aama...@ameripath.com |
www.AmeriPath.com

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