[Histonet] RE: embedding forceps

[Jane Weber]

There is also a company that makes great embedding presses called
Histopress. I use them and love them! Check Histopress.com

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[Histonet] embedding forceps

[Jane Weber]

There is a company that makes a great tool for embedding tissue. I have used
it and love it. Their website is Histopress.com Good luck!

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[Histonet] REMOVE FROM LIST

[Kim Stanley]

Please remove this email address from this list.

Thank you,
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[Histonet] NY cert requirements

[Pitts, Jaclyn S. (Jackie), HT(ASCP)]

Hello, 
My colleague and I have been wondering what the certification
requirements are in New York and what other kind of guidelines that have
to be followed for the state as well in the lab. 

Jaclyn Pitts, HT(ASCP)
Histotechnician
E-mail: pitts.jac...@mayo.edu

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[Histonet] P16 and ProExC Substitutions

[Rick and Sue Boen]

Hi All,

 

Now that MTM has made P16 very expensive and TriPath is have problems
producing ProExC, what's everybody using on ffpe tissue? We were told by
TriPath/BD that ProExC will probably not be available until late June.

 

Rick Boen, BS, HTL(ASCP)

 

St. Luke's Hospital

901 East 1st Street

Duluth, Mn 55805

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[Histonet] Used Laboratory Equipment

[Leslie Chen]

Hi,

Does anyone which are better websites to place ads to sell laboratory
equipment that are reliable and well-trafficked?  We are looking to sell a
Tecan machine.  Thank you!

Leslie
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Re: [Histonet] Re:2

[V. Neubert]

Redirects to a site which offers pills (I cannot even see what pills...)

> ...I hope you’ll enjoy after visiting this site.  
> http://kristiang.kr.funpic.de/page.php?mafortune=65m5
>
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[Histonet] Re:2

[robert king]

...I hope you’ll enjoy after visiting this site.  
http://kristiang.kr.funpic.de/page.php?mafortune=65m5

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[Histonet] RE: Immuno's

[Goins, Tresa]

Valerie -

All of our IHC stains are done manually.  
As an animal tissue lab, our antibody sources will differ, but the best 
chromagen I have use by far is Biocare's Vulcan Fast Red.  I have tried other 
sources (cheaper) for the new fuchsin chromagen, but they do not produce the 
same vivid red stain as Vulcan.

Our secondary antibodies are from KPL (biotinylated anti-mouse or anti-rabbit) 
with KPL alkaline phosphatase.

Good luck


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Monday, April 11, 2011 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immuno's

Hi Folks..
 
Looking for a bit of help. Is there anyone out there who are doing their
immuno's manually?  Our Pathologist has just informed us that our
immuno's are ALL too weak.
He states that they always have been, as he compares them to slides that
we get back from the  reference labs that we use for the antibodies that
we don't do in-house.
 
If you are doing them manually...what companies are you using for
antibodies, detection kit and chromogen? And are your stains really
dark?
 
I informed our Pathologist that I know for a fact that the reference
labs that we use, do the immuno's on stainers, and naturally these
stains will be consistently be stronger.
 
I am trying to see if I can keep costs down by trying not to have to buy
a stainer and continue to do the immuno's manually.
 
If you all know of a wet-workshop or of a immuno. company who would send
someone to our facility to teach us ( the latter is preferred, since my
section chief is going to retire in 1 1/2 weeks, I will be taking over
her duties and this is going to leave us with only myself and one other
tech, until we can get a replacement for my position).
 
Any help or advice that can be given will be greatly appreciated.
 
Thanks so much.
 
Valerie Hannen, MLT(ASCP),HTL,SU (FL)
Parrish Medical Center
Titusville,Florida


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[Histonet] Ken Marissael is out of the office

[Ken_Marissael]

I will be out of the office starting  04/11/2011 and will not return until
04/15/2011.

I will be unavailable and will have  limited access to phone and e-mail.
While I am away, please contact VWR Customer Service at 800-932-5000.


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[Histonet] Gary Steinke is out of the office

[Gary_Steinke]

I will be out of the office starting  04/11/2011 and will not return until
04/18/2011.

I will be out of the office from April 11th and returning on April 18th.  I
will out of town attending a business meeting and will have very limited
access to email.  If you need assistance immediately, please contact
Customer Service at 800-932-5000.  Take care and have a great day.


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[Histonet] 1 H&E slide vs. 2

[Eugenia Thomas]

Good afternoon everyone,
 
Does anyone know of an article or statistics discussing the impact (medical 
diagnosing) of cutting 2 H&E slides per block verses 1 for all routine work?
 
 
Genia 
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AW: [Histonet] Benchmark Ultra question

[Gudrun Lang]

Yes, that could be true. Some of the PrepKits "go harder" than others.
Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles
are not filled equally. (We press always reagens in the nozzle when we
prepare the run.)

With the Ventana-System there is an additional dilution, because the
working-solution is added to a reaction-buffer film under the LCS. Perhaps
it makes a difference if you dispense the solution with the pipett-tip under
the LCS or if the drop falls on the surface of the LCS.  That could be a
matter of the LCS-quality, or buffer-quantity , or . I think I'll become
mad!

Our pathologists have an uncertain unhappiness with the overall staining
results, but most of the antibodies work well.  Perhaps the majority isn't
very sensitiv to small changes in the system and the quality-difference
isn't big enough, but some are easier to "kill".

Regards, Gudrun

 

 

  _  

Von: Angela Bitting [mailto:akbitt...@geisinger.edu] 
Gesendet: Montag, 11. April 2011 18:06
An: gu.l...@gmx.at
Betreff: Re: [Histonet] Benchmark Ultra question

 

When I was trained to do titration runs on the BenchmarkXT and Ultras, I was
told to titrate 100ul of antibody. I have been suspecting for some time now
that the dispensers DON'T dispense a full 100ul. I haven't taken the time to
prove it, but your question may have motivated me.

That could explain the weak staining. 

>>> "Gudrun Lang"  4/11/2011 11:48 AM >>>
Hi!

I have some issues with our new Ultra. Since a few days antibodies, that
work usually well, show up very faint. For example the TTF1 (Novocastra).

I ordered a new bottle, made a titration and found, that the "old" titer
1:50 was again well enough. I filled the old PrepKit - and the result was
very weak.

Then I thought, the PrepKit itself is the culprit. I changed the PrepKit  -
and again the result was very weak. At that time the working-solution was
only three days old.



Has anyone an explanation, why the titration works well and the automated
dispension doesn't?



Regards

Gudrun Lang

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IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
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RE: [Histonet] Immuno's

[Cynthia Pyse]

Do you use a hydrophobic pen to circle the tissue. This concentrates the
solution where you need it. 

Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
716-250-9235 etx.232
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen,
Valerie
Sent: Monday, April 11, 2011 10:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immuno's

Hi Folks..
 
Looking for a bit of help. Is there anyone out there who are doing their
immuno's manually?  Our Pathologist has just informed us that our
immuno's are ALL too weak.
He states that they always have been, as he compares them to slides that
we get back from the  reference labs that we use for the antibodies that
we don't do in-house.
 
If you are doing them manually...what companies are you using for
antibodies, detection kit and chromogen? And are your stains really
dark?
 
I informed our Pathologist that I know for a fact that the reference
labs that we use, do the immuno's on stainers, and naturally these
stains will be consistently be stronger.
 
I am trying to see if I can keep costs down by trying not to have to buy
a stainer and continue to do the immuno's manually.
 
If you all know of a wet-workshop or of a immuno. company who would send
someone to our facility to teach us ( the latter is preferred, since my
section chief is going to retire in 1 1/2 weeks, I will be taking over
her duties and this is going to leave us with only myself and one other
tech, until we can get a replacement for my position).
 
Any help or advice that can be given will be greatly appreciated.
 
Thanks so much.
 
Valerie Hannen, MLT(ASCP),HTL,SU (FL)
Parrish Medical Center
Titusville,Florida


**
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under applicable law. If the reader of this email is not the
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[Histonet] Histology Manager Needed in NY

[Alyssa]

Hello Everyone,

 

I am looking for a Night Shift Histology Manager in New York. The position
is in the White Plains area. Message me for details! Thank you!

 

Alyssa Peterson, Candidate Recruitment

LinkedIn: http://www.linkedin.com/in/alyssapetersonasp 

 Allied Search Partners

T: 888.388.7571 

F: 888.388.7572

www.alliedsearchpartners.com 
 

 

This email including its attachments is intended only for the confidential
use of the individual to whom it is addressed. If you are not the intended
recipient, any use, dissemination, distribution or copying of this message
or its attachments is prohibited.  If you have received this message in
error, please notify us immediately, and delete this message and its
attachments permanently from your system.

 

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[Histonet] Benchmark Ultra question

[Gudrun Lang]

Hi!

I have some issues with our new Ultra. Since a few days antibodies, that
work usually well, show up very faint. For example the TTF1 (Novocastra).

I ordered a new bottle, made a titration and found, that the "old" titer
1:50 was again well enough. I filled the old PrepKit - and the result was
very weak.

Then I thought, the PrepKit itself is the culprit. I changed the PrepKit  -
and again the result was very weak. At that time the working-solution was
only three days old.

 

Has anyone an explanation, why the titration works well and the automated
dispension doesn't?

 

Regards

Gudrun Lang

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RE: [Histonet] Immuno's

[William Chappell]

Toni beat me to it.

My suggestion is to contact the vendor where you purchase your detection.  The 
technical support departments for immuno vendors are emmensely helpful.  Try 
Dako at 800-400-DAKO, Biocare at 603-925-8000, or Cellmarque at 800.665.7284.  

In addition, it has always been my experience that a good hand stain is always 
better than an instrument stain -- primarily because they are better washed by 
hand.  My best guess is that you are not draining your slides well enough 
between rinses.  What I do is flood my slides with buffer, then put them at an 
angle (the Biocare IQ stainer is great for this) and rinse again -- Robin 
Simpkins at Biocare calls this the Jamaican waterfall.  I then follow up with a 
flick of the slide, then proceed to the next step.

Again call your vendors.  Technical support will be extremely helpful and you 
may get an expert (applications specialist or TSR) out to help you in person.

William Chappell, HTL(ASCP)QIHC
IHC Development Specialist
CellNetix Pathology & Laboratories
(503) 358-9567
www.cellnetix.com
"Our expertise...your peace of mind"




--- On Mon, 4/11/11, Rathborne, Toni  wrote:

> From: Rathborne, Toni 
> Subject: RE: [Histonet] Immuno's
> To: "Hannen, Valerie" , 
> histonet@lists.utsouthwestern.edu
> Date: Monday, April 11, 2011, 8:38 AM
> Who are you purchasing your
> antibodies from? Any of the companies that I have dealt with
> would gladly send someone out to optimize their antibodies.
> The fact that you are performing them manually probably is
> not the cause for the weak staining. We used to use the old
> Shandon Sequenza staining system as a backup if our Dako was
> out of service. When used with the same protocols, the
> staining was the same. 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]On
> Behalf Of Hannen,
> Valerie
> Sent: Monday, April 11, 2011 10:26 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Immuno's
> 
> 
> Hi Folks..
>  
> Looking for a bit of help. Is there anyone out there who
> are doing their
> immuno's manually?  Our Pathologist has just informed
> us that our
> immuno's are ALL too weak.
> He states that they always have been, as he compares them
> to slides that
> we get back from the  reference labs that we use for
> the antibodies that
> we don't do in-house.
>  
> If you are doing them manually...what companies are you
> using for
> antibodies, detection kit and chromogen? And are your
> stains really
> dark?
>  
> I informed our Pathologist that I know for a fact that the
> reference
> labs that we use, do the immuno's on stainers, and
> naturally these
> stains will be consistently be stronger.
>  
> I am trying to see if I can keep costs down by trying not
> to have to buy
> a stainer and continue to do the immuno's manually.
>  
> If you all know of a wet-workshop or of a immuno. company
> who would send
> someone to our facility to teach us ( the latter is
> preferred, since my
> section chief is going to retire in 1 1/2 weeks, I will be
> taking over
> her duties and this is going to leave us with only myself
> and one other
> tech, until we can get a replacement for my position).
>  
> Any help or advice that can be given will be greatly
> appreciated.
>  
> Thanks so much.
>  
> Valerie Hannen, MLT(ASCP),HTL,SU (FL)
> Parrish Medical Center
> Titusville,Florida
> 
> 
> **
> "This email is intended solely for the use of the
> individual to
> whom it is addressed and may contain information that is
> privileged, confidential or otherwise exempt from
> disclosure
> under applicable law. If the reader of this email is not
> the
> intended recipient or the employee or agent responsible
> for
> delivering the message to the intended recipient, you are
> hereby notified that any dissemination, distribution, or
> copying of this communication is strictly prohibited. If
> you
> have received this communication in error, please
> immediately
> delete this message. Thank you"
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> by e-mail or yo

RE: [Histonet] Immuno's

[Rathborne, Toni]

Who are you purchasing your antibodies from? Any of the companies that I have 
dealt with would gladly send someone out to optimize their antibodies. The fact 
that you are performing them manually probably is not the cause for the weak 
staining. We used to use the old Shandon Sequenza staining system as a backup 
if our Dako was out of service. When used with the same protocols, the staining 
was the same. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Hannen,
Valerie
Sent: Monday, April 11, 2011 10:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immuno's


Hi Folks..
 
Looking for a bit of help. Is there anyone out there who are doing their
immuno's manually?  Our Pathologist has just informed us that our
immuno's are ALL too weak.
He states that they always have been, as he compares them to slides that
we get back from the  reference labs that we use for the antibodies that
we don't do in-house.
 
If you are doing them manually...what companies are you using for
antibodies, detection kit and chromogen? And are your stains really
dark?
 
I informed our Pathologist that I know for a fact that the reference
labs that we use, do the immuno's on stainers, and naturally these
stains will be consistently be stronger.
 
I am trying to see if I can keep costs down by trying not to have to buy
a stainer and continue to do the immuno's manually.
 
If you all know of a wet-workshop or of a immuno. company who would send
someone to our facility to teach us ( the latter is preferred, since my
section chief is going to retire in 1 1/2 weeks, I will be taking over
her duties and this is going to leave us with only myself and one other
tech, until we can get a replacement for my position).
 
Any help or advice that can be given will be greatly appreciated.
 
Thanks so much.
 
Valerie Hannen, MLT(ASCP),HTL,SU (FL)
Parrish Medical Center
Titusville,Florida


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[Histonet] Unsubscribe

[Kaylee McCaffrey]

Unsubscribe.


Kaylee McCaffrey
MedWrench Product Manager



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RE: [Histonet] RE: embedding forceps

[Rathborne, Toni]

We also use Mopec. They come in a few sizes, and our rep has worked with us to 
find something that I was having a hard time locating.

http://www.mopec.com/category/947/ergonomic/

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Horn,
Hazel V
Sent: Monday, April 11, 2011 11:06 AM
To: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; Gale Limron;
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embedding forceps


These are the same ones we like.  We like the 7" ones.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Monday, April 11, 2011 7:32 AM
To: Gale Limron; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embedding forceps

I like these:

You can get them in different lengths.5 1/2" or 7"  

  
http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron
Sent: Monday, April 11, 2011 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding forceps

Good Morning!
Can anyone recommend comfortable, not-heated, embedding forceps (with ordering 
info)? Our favorite that we had for years disappeared a while back and we 
haven't found a replacement that we really like yet.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



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[Histonet] RE: embedding forceps

[Horn, Hazel V]

These are the same ones we like.  We like the 7" ones.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Monday, April 11, 2011 7:32 AM
To: Gale Limron; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embedding forceps

I like these:

You can get them in different lengths.5 1/2" or 7"  

  
http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron
Sent: Monday, April 11, 2011 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding forceps

Good Morning!
Can anyone recommend comfortable, not-heated, embedding forceps (with ordering 
info)? Our favorite that we had for years disappeared a while back and we 
haven't found a replacement that we really like yet.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed 
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RE: [Histonet] science for kids

[Rathborne, Toni]

So would I!


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Sherwood,
Margaret 
Sent: Monday, April 11, 2011 10:25 AM
To: Patsy Ruegg; Margaret Blount; Pam Barker; Emily Sours
Cc: Histonet
Subject: RE: [Histonet] science for kids


Patsy,

I would appreciate receiving the flyers as well.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Saturday, April 09, 2011 12:04 PM
To: 'Margaret Blount'; 'Pam Barker'; 'Emily Sours'
Cc: 'Histonet'
Subject: RE: [Histonet] science for kids

Years ago ASCP I believe gave out coloring books for histology, or was it
NSH?  I got a couple for my grandkids, they love it, my 7 year old
granddaughter just pulled it out and was talking to me about it last week.
I have some flyers on histology I could email to you as well, I pass these
out when I talk to kids about careers in histology.  Please contact me
directly at work and I will email them to you, I scanned them into my
computer so it will be easy to send them to you.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret
Blount
Sent: Wednesday, April 06, 2011 1:55 AM
To: Pam Barker; Emily Sours
Cc: Histonet
Subject: RE: [Histonet] science for kids

Me too, even in the UK!

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam
Barker
Sent: 05 April 2011 20:25
To: 'Emily Sours'
Cc: 'Histonet'
Subject: RE: [Histonet] science for kids

Me TOO How do we get them? 


Thank You!
  
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Tuesday, April 05, 2011 3:19 PM
To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] science for kids

A coloring book?! I want that for myself!!!

A great book should leave you with many experiences, and slightly
exhausted.
You should live several lives while reading it.
-William Styron



On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) <
algra...@email.arizona.edu> wrote:

> Emily,
>
> NSH has a coloring book that explains histology.
>
>
> Andi Grantham
>
>
>
> On Apr 5, 2011, at 7:14 AM, Emily Sours wrote:
>
> > I'm going to visit my nieces this summer and they want to know what 
> > I do
> at
> > work.  Does anyone know of a good book or website that explains DNA
> and/or
> > histology for 4 to 6 year olds? Just thought I'd throw it out there,
> since
> > googling it would yield way more than I can deal with.
> >
> > Emily
> >
> > A great book should leave you with many experiences, and slightly
> exhausted.
> > You should live several lives while reading it.
> > -William Styron
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
___
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The information in this e-mail is intended only for the person to whom it is
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contains patient information, please contact the Partners Compliance HelpLine at
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[Histonet] Immuno's

[Hannen, Valerie]

Hi Folks..
 
Looking for a bit of help. Is there anyone out there who are doing their
immuno's manually?  Our Pathologist has just informed us that our
immuno's are ALL too weak.
He states that they always have been, as he compares them to slides that
we get back from the  reference labs that we use for the antibodies that
we don't do in-house.
 
If you are doing them manually...what companies are you using for
antibodies, detection kit and chromogen? And are your stains really
dark?
 
I informed our Pathologist that I know for a fact that the reference
labs that we use, do the immuno's on stainers, and naturally these
stains will be consistently be stronger.
 
I am trying to see if I can keep costs down by trying not to have to buy
a stainer and continue to do the immuno's manually.
 
If you all know of a wet-workshop or of a immuno. company who would send
someone to our facility to teach us ( the latter is preferred, since my
section chief is going to retire in 1 1/2 weeks, I will be taking over
her duties and this is going to leave us with only myself and one other
tech, until we can get a replacement for my position).
 
Any help or advice that can be given will be greatly appreciated.
 
Thanks so much.
 
Valerie Hannen, MLT(ASCP),HTL,SU (FL)
Parrish Medical Center
Titusville,Florida


**
"This email is intended solely for the use of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you
have received this communication in error, please immediately
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RE: [Histonet] science for kids

[Sherwood, Margaret]

Patsy,

I would appreciate receiving the flyers as well.

Peggy 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Saturday, April 09, 2011 12:04 PM
To: 'Margaret Blount'; 'Pam Barker'; 'Emily Sours'
Cc: 'Histonet'
Subject: RE: [Histonet] science for kids

Years ago ASCP I believe gave out coloring books for histology, or was it
NSH?  I got a couple for my grandkids, they love it, my 7 year old
granddaughter just pulled it out and was talking to me about it last week.
I have some flyers on histology I could email to you as well, I pass these
out when I talk to kids about careers in histology.  Please contact me
directly at work and I will email them to you, I scanned them into my
computer so it will be easy to send them to you.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret
Blount
Sent: Wednesday, April 06, 2011 1:55 AM
To: Pam Barker; Emily Sours
Cc: Histonet
Subject: RE: [Histonet] science for kids

Me too, even in the UK!

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam
Barker
Sent: 05 April 2011 20:25
To: 'Emily Sours'
Cc: 'Histonet'
Subject: RE: [Histonet] science for kids

Me TOO How do we get them? 


Thank You!
  
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Tuesday, April 05, 2011 3:19 PM
To: Grantham, Andrea L - (algranth); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] science for kids

A coloring book?! I want that for myself!!!

A great book should leave you with many experiences, and slightly
exhausted.
You should live several lives while reading it.
-William Styron



On Tue, Apr 5, 2011 at 1:49 PM, Grantham, Andrea L - (algranth) <
algra...@email.arizona.edu> wrote:

> Emily,
>
> NSH has a coloring book that explains histology.
>
>
> Andi Grantham
>
>
>
> On Apr 5, 2011, at 7:14 AM, Emily Sours wrote:
>
> > I'm going to visit my nieces this summer and they want to know what 
> > I do
> at
> > work.  Does anyone know of a good book or website that explains DNA
> and/or
> > histology for 4 to 6 year olds? Just thought I'd throw it out there,
> since
> > googling it would yield way more than I can deal with.
> >
> > Emily
> >
> > A great book should leave you with many experiences, and slightly
> exhausted.
> > You should live several lives while reading it.
> > -William Styron
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
___
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


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[Histonet] RE: embedding forceps

[Bartlett, Jeanine (CDC/OID/NCEZID)]

I like these:

You can get them in different lengths.5 1/2" or 7"  

  
http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron
Sent: Monday, April 11, 2011 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding forceps

Good Morning!
Can anyone recommend comfortable, not-heated, embedding forceps (with ordering 
info)? Our favorite that we had for years disappeared a while back and we 
haven't found a replacement that we really like yet.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed 
and may contain information that is privileged, confidential or otherwise 
protected from disclosure. Dissemination, distribution or copying of this 
e-mail or the information herein by anyone other than the intended recipient, 
or an employee or agent responsible for delivering the message to the intended 
recipient, is prohibited. If you received this message in error, please delete 
without copying and kindly e-mail a reply to inform us of the mistake in 
delivery.
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[Histonet] embedding forceps

[Gale Limron]

Good Morning!
Can anyone recommend comfortable, not-heated, embedding forceps (with ordering 
info)? Our favorite that we had for years disappeared a while back and we 
haven't found a replacement that we really like yet.
Thank you,
Gale

Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562



This e-mail is intended only for the person or entity to which it is addressed 
and may contain information that is privileged, confidential or otherwise 
protected from disclosure. Dissemination, distribution or copying of this 
e-mail or the information herein by anyone other than the intended recipient, 
or an employee or agent responsible for delivering the message to the intended 
recipient, is prohibited. If you received this message in error, please delete 
without copying and kindly e-mail a reply to inform us of the mistake in 
delivery.
___
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