[Histonet] RE: Thanks to all!!!!!
Thanks to all! I am writing this as a thanks to all persons posing questions and responding to them on the digest. I am an Education Coordinator at MD Anderson School of Health Professions and we are implementing a Critical Thinking component to our classes. You would not realize how difficult it is to come up with those type of questions on demand. Brain farts as I call them. The digest will provide me with great ideas to create questions to teach troubleshooting and critical thinking skills to the students. Skills that are sometimes lacking. Some questions just lead me to come up with ones to pose to the students. Staining questions about the H & E are the best. ALWAYS, THE IDENTIFYING INFORMATION WILL BE LEFT OUT TO PROTECT CONFIDENTIALITY.(HIPPA) I appreciate the knowledge and experience that is found here and respect it highly. I have been able to re-connect with long lost co-workers and to pass on any experience that I have to others. Thanks again, Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tumors tumors everywhere
Sarah, I am curious; have you tried contacting the BioCare research tech support reps? They are very helpful regarding their products and trouble-shooting any issues that may arise. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 28, 2011, at 1:16 PM, wrote: So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tumors tumors everywhere
So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Probes resolution
Thanks to all of you who offered your help. I did have a conference call with Ventana this afternoon and all my questions were answered. I'll share with you all what I know so no one else needs to fret needlessly. I didn't receive 2- 4ml vials of EBER Probe. I only received 1 when I ordered it. When you order the 760-1209, you should get 2 vials of Probe 4 ml each. I will be getting the second vial sent out to me tomorrow. So now I will have enough Probe to fill my ISH prep kit. It was a production/shipping error. If Customer Support could have explained that to me this morning, I would never have been so frustrated. Water under the bridge. I have put Ventana back on my Christmas card list. Also, they cannot give you the "recipe" but if your "recipe" doesn't work, a TAS can tell you what to try to fix it. I hope this info is helpful. Angie That's what I know, so thought I'd share. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Supplier for human testis control slides?
I can think of a couple of people I would gladly volunteer. Lynne Bell, HT (ASCP) Histology Team Leader 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Supplier for human testis control slides?
I'm working up a new antibody and human testis is the suggested postive control. Does anyone know of a supplier? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recycling slide brite
Looks like B/R has a gadget that will do it... http://www.solvent--recycling.com/xylene_substitutes.html Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Tue, 26 Apr 2011 12:51:15 -0700 > From: rjbu...@yahoo.com > To: Histonet@lists.utsouthwestern.edu; dwenze...@gmail.com > Subject: Re: [Histonet] Recycling slide brite > CC: > > Being a MIXTURE of hydrocarbons, it will crack at different temperatures > (separately). You would have to reconstitute it using the same proportions > (usually "proprietary"). > René J. > > --- On Tue, 4/26/11, Dawn Herron wrote: > > > From: Dawn Herron > Subject: [Histonet] Recycling slide brite > To: Histonet@lists.utsouthwestern.edu > Date: Tuesday, April 26, 2011, 2:25 PM > > > Hello histonetters. We have made the transition from xylene to using slide > brite in our stainer and for coverslipping and have worked out all the kinks > except one---recycling it successfully. We've been talking to our recycler > manufacturer (B&R) and the manufacturer of slide brite and still haven't had > a successful run. Does anyone out there use this product and recycle it? If > so could you send me your recycling protocol? Any thoughts would be > appreciated. > > Thanks, > Dawn > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IMEB
Since my Navy time I have had two major careers in my life time. Histology and Sales(17 yrs). My experience gained me this prospective: All companies go through periods were everything they do is done right, then a few key people leave and suddenly they can't get out of their own way. Sometimes they figure it out and get back on track and sometimes they don't and fail. I am not familiar with IMEB at all and cannot comment on their status. I will comment on what I always told my staff. "It is unforgivable not to call a customer back, no matter how bad the news is". "Never lie, that way you don't have to remember what you said the last time you talked to them". "Remember this, a bad experience of any kind with us travels faster that a good one". "Above all never ever lie to me". We had customers that loved us because we did what we said were going to do and others that hated us because sometimes we just could not get out of our own way. By the way, I left my last sales position because of the President of Sales (minor owner), would not call back customers and would downright lie to them. He was later bought out and terminated. Thus my return to Histology. I think the only point I am trying to make is that there is always two sides to the story. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 28, 2011 10:52 AM To: Sheila Haas; histonet@lists.utsouthwestern.edu; Kaye Ryan Subject: RE: [Histonet] IMEB Kaye: In no way this posting is aimed at "roasting" yours, not at all, it is just to explain MY philosophy when participating in HistoNet. When I read a posting first I consider if I have something useful to contribute and if that is the case I post my answer and I really do not care if it will follow a "consensus", it is my answer based on my experience and from the start I consider that I may be wrong or that others' answers will reflect reality in a better way. The fact that you may have a good commercial experience with IMEB in absolutely no way excludes the possibility that others' experiences could have been disastrous. Additionally, it does not really matter how others have related to this company if the one posting the initial query has had a bad experience. Summing up do as I do: post your answer if you consider will help, and absolutely disregard what others may think of it, and never try to impose your criterion. René J. --- On Thu, 4/28/11, Kaye Ryan wrote: From: Kaye Ryan Subject: RE: [Histonet] IMEB To: "Sheila Haas" , histonet@lists.utsouthwestern.edu Date: Thursday, April 28, 2011, 10:32 AM I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks to everyone for the feedback on IMEB. It appears there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire dep
Re: [Histonet] Re: C4d stain for IF
Try the C4d directly labeled antibody from alpco www.alpco.com anti human C4d (FITC-Conjugated) Cat #: 04-BI-RC4D-FITC Good luck. On Wed, Apr 27, 2011 at 2:54 PM, Butler, Roszetta wrote: > I'm having trouble with my C4d IF stain. I'm using AbDirect/Serotec, mouse > monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec > diluted 1:25 for ½ hour. This is not working and I've tried different > dilutions with no success. Can anyone share their protocol? > > Rosie Butler, > AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK > 73112/405.842.7575 - Office > 405.650.8921 - Cell/405.841.2002 - Fax > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TNF-alpha
I was incorrect in my earlier post, we actually use the antibody from Sigma for TNF- alpha, I was thinking of IL-6 that's the antibody we get from abcam Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TNF alpha antibody
We have used the antibody from abcam on FFPE mouse tissue with good success, have not tired it on frozen sections Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, April 28, 2011 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TNF alpha antibody Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue? Any suggestions are greatly appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TNF alpha antibody
Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue? Any suggestions are greatly appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Probes
At 100µL per dispense, to do 50 tests you will need 50 x 100µL = 5000 µL = 5 ml You do need to dilute it René J. --- On Thu, 4/28/11, Angela Bitting wrote: From: Angela Bitting Subject: Re: [Histonet] Ventana Probes To: "Angela Bitting" , "histonet" Date: Thursday, April 28, 2011, 11:10 AM Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? >>> "Angela Bitting" 4/28/2011 11:03 AM >>> Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rantanyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Probes
Before adding vodka, take some "sips". René J. --- On Thu, 4/28/11, Angela Bitting wrote: From: Angela Bitting Subject: [Histonet] Ventana Probes To: "histonet" Date: Thursday, April 28, 2011, 11:03 AM Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rantanyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Probes
Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? >>> "Angela Bitting" 4/28/2011 11:03 AM >>> Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rantanyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Probes
Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rantanyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IMEB
Kaye: In no way this posting is aimed at "roasting" yours, not at all, it is just to explain MY philosophy when participating in HistoNet. When I read a posting first I consider if I have something useful to contribute and if that is the case I post my answer and I really do not care if it will follow a "consensus", it is my answer based on my experience and from the start I consider that I may be wrong or that others' answers will reflect reality in a better way. The fact that you may have a good commercial experience with IMEB in absolutely no way excludes the possibility that others' experiences could have been disastrous. Additionally, it does not really matter how others have related to this company if the one posting the initial query has had a bad experience. Summing up do as I do: post your answer if you consider will help, and absolutely disregard what others may think of it, and never try to impose your criterion. René J. --- On Thu, 4/28/11, Kaye Ryan wrote: From: Kaye Ryan Subject: RE: [Histonet] IMEB To: "Sheila Haas" , histonet@lists.utsouthwestern.edu Date: Thursday, April 28, 2011, 10:32 AM I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks to everyone for the feedback on IMEB. It appears there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IMEB
It would clear things up quite a bit if they would simply return her call. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Thursday, April 28, 2011 10:46 AM To: kr...@nfderm.com; micropathl...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IMEB I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY I will now step down off my box =) Have a Happy Thursday Histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Thursday, April 28, 2011 9:32 AM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IMEB I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks to everyone for the feedback on IMEB. It appears there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___
Re: [Histonet] IMEB
Kaye, I was telling of my situation and asked if others had recent problems. I received many responses with two being positive and most of the others negative (a couple were offering suggestions on how to handle). I have heard this company was very reputable which is why I chose to do business with them in the first place but have since heard that recently things have been quite rocky. I do feel, at this point, that others should be leary before ordering from this company. I'd hate for someone else to be in the same situation as we are, particularly during these difficult economic times (we are out a large sum of money at this point). I also stated that if the issue is resolved, I would let everyone know but up until now it has not been. You are right, we did not have an account with IMEB but what was promised in the beginning is not what played out. I have been in this field for a very long time and realize how the accounting works but I also realize that a full refund is not unreasonable given I jumped through every hoop they requested I jump through. Keep in mind, restocking fees typically occur when items are already shipped or delivered to the customer and they return them. This is not the case here, they have both the item and our money. Again, if this issue is resolved to my satisfaction, I will gladly let everyone know. Sheila Haas From: Kaye Ryan To: Sheila Haas ; histonet@lists.utsouthwestern.edu Sent: Thu, April 28, 2011 10:32:22 AM Subject: RE: [Histonet] IMEB I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks to everyone for the feedback on IMEB. It appears there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IMEB
I agree Kaye!! IMEB has always gone above and beyond to help me with anything I have ever needed. They even had one of their techs call and help me troubleshoot some equipment that wasn't even theirs! I can never say enough awesome things about Denise either!! She is AWESOME!! Keeps in contact and tries to help out in any way she can!! I bought a used processor from them and a few months later it had an issue. They sent someone to my lab (on their dime by the way) who fixed it on the spot! From the time I called to the time it was fixed was less than 2 days!! It was an old VIP and the repair tech even made his own diagnostic equipment and repair parts! I would recommend this company to anyone (and have!!). It is a shame that one person's bad experience has been broadcast over the histonet for the world to see. With anything in this world there is always going to be one person who has a bad taste in their mouth about something. Because people always yell out the bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY I will now step down off my box =) Have a Happy Thursday Histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Thursday, April 28, 2011 9:32 AM To: Sheila Haas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IMEB I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks to everyone for the feedback on IMEB. It appears there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IMEB
I usually do not respond to postings because I have seen people that may not agree with a posting get "roasted" by others but I felt like I had to chance it with this posting. I have had many excellent transactions with IMEB and would recommend them to anyone. Their products are trustworthy and their prices are excellent compared to other companies that sell the same products. I have known the owner and several of the reps for many years and have never heard of an incident like this. I too, had to put a deposit down when I made my first order because we did not have an established account with them. I am assuming that you did not have an account already established since they were checking your credit status? It does take time to fill out the paper work and for them to check your credit references. It is that way with almost any new company that you place a large order with. In this day and time "trust" is a hard thing to come by on a new account. It is terrible that people that may not have used them now have a negative thought in their minds about the company. We all have at some time something that we can find not quite to our liking about any company and I think we need to be careful when we are saying statements like " It appears there have only been a couple of successful recent transactions with them". This can be interpreted by some to mean that there have been many negative interactions so stay clear of them. Maybe a lot of their customers do not reply to posting or do not even subscribe to the histonet. Kaye Ryan Histology Manager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, April 28, 2011 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IMEB Thanks to everyone for the feedback on IMEB. It appears there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IMEB
Thanks to everyone for the feedback on IMEB. It appears there have only been a couple of successful recent transactions with them. Most of the feedback I received was not favorable so it appears the company is in a good bit of disarray for reasons unknown. At this point, I've not gotten a response from the owner regarding the return of our entire deposit but if he actually comes through, I will let you all know. In the mean time, be leary of future transactions with them. Thanks again for the feedback. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] MSH 2011 Symposium
Hi Amber - I have the MSH Symposium scheduled to begin promos on May 8th's HistoTALK. Hopefully, the announcements will help get listeners attention and get them to attend. I'll be sure to mention your email on the show. :- ) Yours, Dave www.HistoTALK.com From: Amber McKenzie Cc: Histonet list serv. Sent: Wed, April 27, 2011 12:08:14 PM Subject: [Histonet] MSH 2011 Symposium If anyone is interested in attending the annual Mississippi Society for Histotechnology Symposium June 24-26th in Hattiesburg, MS, please contact me directly and I can email you a program! We'd love to have you :) Amber McKenzie, BS, HT(ASCP) MSH Secretary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] chicken immunohistochemistry
dear all i'm looking for vendors or companies that we could order some anti chicken antibodies (primary & secondary). speacially for CD4 & CD8 i'm new in that feild and need your help. mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
