[Histonet] Histology station for core lab

[Debra Baluch]

Hi All,
 I manage a bioimaging core facility and teach a couple of courses related 
to cell and tissue culture research and sample prep for live and fixed cell 
imaging. We have expanded to train people on the use of a cryostat, that is 
part of our core facility, and are now looking into obtaining a microtome to 
teach histological techniques.  Do you have recommendations of a good rotary 
microtome that would work well in a core facility? Most researchers in our 
department manually embed and stain so we are not as familiar with the more 
automated instruments for sample processing. Since much of the focus is in 
regards to learning the process we are leaning towards going with the more 
manual route. We are working with a limited budget so we will need to be 
somewhat conservative in the models we select.
Thanks for your suggestions,
Page

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] motorized cryostat

[Ed Roy]

I have a small research lab with an old (very old) Minot IEC microtome 
cryostat, which is on its last legs. In starting to shop for a new one, 
I was wondering how much difference a motorized one would make.  We do 
mostly fresh frozen (unfixed) mouse brain and lymph node sections. Are 
the sections any better?
Thanks for any opinions on this and any recommendations on a good 
cryostat at a reasonable cost.


Ed Roy

--

Edward Roy, PhD
Professor of Pathology
Professor of Molecular and Integrative Physiology
University of Illinois at Urbana-Champaign
506 S. Mathews Ave.
Urbana, IL 61801
217 333-3375


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Compliments to the team

[Doug Showers]

Dr. Barrett sends his thanks to the Histology team for a job well done today.



Doug Showers, MS, HT

Histology Manager

ProPath

1355 River Bend

Dallas, TX 75247

214-237-1680

214-422-3083 Mobile



To learn more about ProPath, please visit http://www.ProPathLab.com





The information contained in this message may be privileged and confidential. 
If you are NOT the intended recipient, please notify the sender immediately 
with a copy to postmas...@propath.com and destroy this message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] NAACLS accredited Histotechnician program

[Johnson, Nacaela]

Does anyone know of an online program for HT that is NAACLS accredited?
 

Thanks,

 

Nacaela Johnson, B.S. HTL (ASCP)CM

Histotechnologist

KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  nacaela.john...@usoncology.com

 
The contents of this electronic mail message and any attachments are 
confidential, possibly privileged and intended for the addressee(s) 
only.Only the addressee(s) may read, disseminate, retain or otherwise use 
this message. If received in error, please immediately inform the sender and 
then delete this message without disclosing its contents to anyone.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] 2011 Louisiana Society for Histotechnology Annual Symposium

[Montina Van Meter]

Dear Histonet Colleagues,

 

The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 3 & 4, 2011, in Baton Rouge, LA.


 

Meeting location:  The Embassy Suites Hotel

 4914 Constitution Ave.

 Baton Rouge, LA  70808  

 

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date has been extended to 

May 20, 2011.  After that date rooms may be reserved at the same special
rate per availability.  The hotel will honor this rate two days prior
and two days after our meeting (please contact the Embassy Suites Hotel
for further information: 1-225-924-6566 or 1-800-Embassy).   

 

A complimentary hotel shuttle service is provided for those flying into
Baton Rouge. Great dining, beautiful southern plantations, and gambling
boats on the Mississippi River are just a few of the attractions in the
Baton Rouge area. Remember to ask for the Louisiana Society for
Histotechnology group when placing your reservation. The LSH will accept
credit cards through our new PayPal account. If you would like to pay
via credit card, please send me your application form and the LSH will
email an invoice to you that allows you to input your card information.
The LSH will also accept check or cash payments. Walk-ins are always
welcome!  

 

If you have any questions or would like a brochure please contact me and
I will send it via email or fax:  

 

Tina Van Meter at 225-603-0953 or vanme...@pbrc.edu

 

 

 

2011 LSH Workshops:

 

1.MOHS Surgery and the Art of Proper Orientation in Embedding

2.In Situ and Assay Development

3.Lab Math, Team Building and Leadership within the Lab.

4.Basic IHC

5.Competency Assessment

6."The Right Stain"

7.Multi-Systems Made Easy - (IHC)

8.Prostate Cancer and Prostate Cancer IHC Markers - Traditional &
New

 

 

Thank you,

 

The LSH Education Committee

 

 

 

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] (no subject)

[Vickroy, Jim]

The C-Kit CD117 antibody from Ventana now has the following FDA approval:


C-Kit has PMA FDA approval along with a companion diagnostic approval to 
utilize Gleevac therapy when positive for GIST tumors.

Since the cost is now very high for the antibody and since it is used for 
predictive therapy is there another CPT code we should be charging these?
Otherwise at our current price for testing we are taking a pretty good kit.   
How are others handling this stain?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



This message (including any attachments) contains confidential information 
intended for a specific individual and purpose, and is protected by law. If you 
are not the intended recipient, you should delete this message. Any disclosure, 
copying, or distribution of this message, or the taking of any action based on 
it, is strictly prohibited.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] nuclear bubbling

[Patsy Ruegg]

This is most likely caused by inadequate fixation in formalin which would
protect the tissues from tissue processing.  With such fast turn around
times we are seeing this more and more.  Put formalin on your tissue
processor and use any extra time you may have up front in formalin rather
than tissue processing.  Ideally it takes 24 hrs to properly fix tissues so
they are not damaged by processing and embedding in paraffin, but nobody
fixes that long anymore.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, May 12, 2011 1:41 PM
To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] nuclear bubbling

Nacaela: 
Heat by itself is not the cause, but the heat applied to sections that have
not been properly drained and still have water left between them and the
slide, is.
You have to drain the sections properly before heating them.
Using formalin is not the cause.
René J.

From: "Johnson, Nacaela" 
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 12, 2011 12:51 PM
Subject: [Histonet] nuclear bubbling

Has anyone one had problems with nuclear bubbling?  I have read about
two different causes.  (1) Formalin itself is known to cause the issue
and (2) heating the tissue at a high temp (70 degrees C or above) while
drying.  The latter is definitely not happening, but I do use formalin.
I am in the process of changing the type of formalin that is used during
collection.  Does anyone have any other suggestions?  Has this problem
occurred for any other reason than I have already pointed out?  It seems
to be worse in the surgical needle biopsies than in the bone marrow
biopsies.


Thanks,



Nacaela Johnson, B.S. HTL (ASCP)CM

Histotechnologist

KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  nacaela.john...@usoncology.com


The contents of this electronic mail message and any attachments are
confidential, possibly privileged and intended for the addressee(s)
only.Only the addressee(s) may read, disseminate, retain or otherwise
use this message. If received in error, please immediately inform the sender
and then delete this message without disclosing its contents to
anyone.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HT Position

[Hale, Meredith]

Great opportunity for a Histotechnician in a brand new laboratory! We
are an 8 physician Dermatology practice located in Bucks County, PA
looking for a certified HT or HTL.  Candidate must meet CLIA grossing
requirements.  The candidate will be responsible for routine histology
duties.  This is a part-time position that offers a competitive salary
and flexible hours. Interested applicants should contact Meredith Hale,
phone (214)596-2219 or e-mail mh...@carisls.com

 

 

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator 

 

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 214-596-7095

mh...@carisls.com   

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CD79 alpha dilemma

[Jan Shivers]

Hi Craig,

I have found CD79a from Biocare (cat. # CM067C) to stain dog, cat, pig, cow, 
horse, sheep, and mouse so far.  Have not tried it on other species yet (in 
other words, I don't have any negative species results to report).


I do have to use HIER with high pH retrieval buffer (Dako; contains EDTA) to 
expose antigens (in a Biocare Decloaking Chamber/pressure cooker).  Citrate 
buffer doesn't do the job well enough.  Microwave oven antigen retrieval 
will also work, but it exposes fewer epitopes than the pressure cooker.


If you have any more questions, feel free to contact me directly.

Jan Shivers
Senior Scientist
IHC/Histology/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

(Confidentiality Notice: This message, together with any attachments, is 
intended only for the use of the individual or entity to which it is 
addressed and may contain confidential or privileged information. If you 
think you have received this message in error, please advise the sender and 
then delete this message and any attachments immediately.)




- Original Message - 
From: "Farish, Craig" 

To: 
Sent: Thursday, May 12, 2011 8:35 PM
Subject: [Histonet] CD79 alpha dilemma


Hi folks - has anyone out there found a Cd79α clone and supplier which will 
cover multiple species reliably?
I can find clones which will stain cats and dogs (and humans), and clones 
which will cover horses, pigs, cows and primates (and humans, but not 
opossums for some reason??) but i'm yet to find one which will cross-react 
with both the large and small animals commonly encountered in a vet lab. I 
would rather not have to stock 2 versions of the same antibody if I can 
avoid it.

Any suggestions would be much appreciated.
As always, thanks to everyone who contributes to histonet,
Craig

Craig Farish
Senior Technical Officer
Veterinary Diagnostic Laboratory
School of Animal and Veterinary Sciences
Charles Sturt University
Boorooma Street
Wagga Wagga
NSW 2678
Australia
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: CD79 alpha dilemma

[Goins, Tresa]

We use Cd79a clone Ab-1 (HM47/A9) from Thermos Scientific (Cat #MS-357).  It 
does not list either cats or dogs on the data sheet but it works.  They also 
suggest HIER-Citrate pH6 for retrieval, but in our hands Citrate pH 9 produces 
better results - steam heat for 20 min and cool on bench top for 30 min - we an 
a non-automated lab.

Good luck,


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Farish, Craig
Sent: Thursday, May 12, 2011 7:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD79 alpha dilemma

Hi folks - has anyone out there found a Cd79α clone and supplier which will 
cover multiple species reliably?
I can find clones which will stain cats and dogs (and humans), and clones which 
will cover horses, pigs, cows and primates (and humans, but not opossums for 
some reason??) but i'm yet to find one which will cross-react with both the 
large and small animals commonly encountered in a vet lab. I would rather not 
have to stock 2 versions of the same antibody if I can avoid it.
Any suggestions would be much appreciated.
As always, thanks to everyone who contributes to histonet,
Craig

Craig Farish
Senior Technical Officer
Veterinary Diagnostic Laboratory
School of Animal and Veterinary Sciences
Charles Sturt University
Boorooma Street
Wagga Wagga
NSW 2678
Australia
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Ventana Kappa/Lambda Probes

[Willis, Nancy F]

We are working up the new Ventana Kappa and Lambda ISH stains. It is
very weak staining on the core and the clot. The RNA positive control
are very good. We are using the ImmunoCal containing formic acid for our
decal solution. Does any one have it working on the core and clot.
Suggestions would be appreciated. 
 
Nancy A.F. Willis, CT(ASCP)
Quest Diagnostics Nichols Institute | Supervisor, Anatomic Pathology
Special Testing| 14225 Newbrook Dr| Chantilly, VA USA | phone
+1.703.802.6900 x65416| fax +1.703.802.7191|
nancy.f.wil...@questdiagnostics.com
mailto:nancy.f.wil...@questdiagnostics.com>  |
www.NicholsInstitute.com http://www.nicholsinstitute.com/>  

Please think about resource conservation before you print this message

 
 


--
The contents of this message, together with any attachments, are
intended only for the use of the person(s) to which they are
addressed and may contain confidential and/or privileged
information. Further, any medical information herein is
confidential and protected by law. It is unlawful for unauthorized
persons to use, review, copy, disclose, or disseminate confidential
medical information. If you are not the intended recipient,
immediately advise the sender and delete this message and any
attachments. Any distribution, or copying of this message, or any
attachment, is prohibited.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Trilogy and Kidney

[Peter Boor]

Hi everyone,

 

We are trying to establish several stainings of GFs and their receptors
(e.g. PDGF/R) in formalin fixed human kidney tissues. In our hands and
according to literature a nice specific staining is seen with Trilogy Ag
retrieval (using pressure cooker). However, using this Ag retrieval,
independent of which primary or secondary Ab we use or how rigorous is our
Av./Biot. And peroxidase blocking, we always get a strong unspecific tubular
background. 

I was wondering if anyone encountered a similar problem and if someone has
any suggestions for this?

Many thanks in advance,

Best regards

Peter

 

P E T E RB O O R,  MD, PhD

Dpt. of Nephrology & Inst. of Pathology, RWTH University Aachen

Pauwelstr. 30

52074 Aachen, Germany

Tel.:+49 241 80 89670

E-Mail: b...@email.cz

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet