[Histonet] chemical waste co

[rmweber113]

Hello,  Does anyone have a name of a laboratory chemical waste company in 
Southern California? 



Thanks for your help. 



Marilynn Weber H.T.( ASCP ) QIHC 
Coastal Pathology Consulting Services LLC 
732 814-1170 
fax 267 722-8308 




Marilynn Weber H.T.( ASCP ) QIHC 
Coastal Pathology Consulting Services LLC 
732 814-1170 
fax 267 722-8308 
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RE: [Histonet] uric acid crystals in tissue for gout

[Rosie Scrimizzi]

You can also do dab smears with the fresh tissue on a slide, pop a coverslip on 
top and examine the wet prep under a polarising microscope. I agree routine 
processing doesn't completely remove UA crystals and an unstained section as 
described below can be used to demonstrate UA

Rosie Scrimizzi
Siemens Australia

> Date: Mon, 16 May 2011 11:06:51 -0700
> From: laurie.colb...@huntingtonhospital.com
> To: dmcc...@ckha.on.ca; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] uric acid crystals in tissue for gout
> CC: 
> 
> We receive these specimens fresh and hand-process through 100% alcohol,
> xylene, and paraffin.  The crystals are water soluble, so you need to
> avoid any aqueous solutions. We cut one slide for routine H&E staining
> and one slide that we just deparaffinize in xylene and then coverslip
> directly out of that xylene.  The pathologist looks at the unstained
> slide with a polarizing lens.  If the tissue gets processed routinely
> (through formalin and the graded alcohols), we still cut the two slides
> and sometimes the crystals are still there.
> Laurie Colbert 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana
> McCaig
> Sent: Monday, May 16, 2011 5:47 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] uric acid crystals in tissue for gout
> 
> Does anyone have a method for identifying uric acid crystals in gout on
> a tissue sample.
> 
>  
> 
> Diana
> 
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Re: [Histonet] sections falling off slides

[Rene J Buesa]

Your test subject is one of the more difficult there are.
Although you may get some background, use some gelatin dissolved in the water 
bath to try to adhere the sections to the slides. Also try to cut as thin as 
you can and make sure there is no water left between the sections and the slide.
Bake at 60ºC for at least 45 minutes before starting the IHC procedure. If you 
use HIER try to switch to pepsin.
The least "stress" you add to the sections the better.
René J.

From: Robin Dean 
To: histonet@lists.utsouthwestern.edu
Sent: Monday, May 16, 2011 2:07 PM
Subject: [Histonet] sections falling off slides

Hi All,



We are trying to do IHC for multiple antigens on goat knees. The sections
are decalcified FFPE sections and we are using a Dako autostainer. We are
currently trying air-drying for  1-2 days  and baking of tissue slides
before use in IHC..

The one previous time we tried baking, we noticed a decrease in IHC signal.
The sections are pretty large, we tried cutting them down, but can't
decrease the size much.  We also  decreased the number of washes and size of
washes on the Daok, which helped only slightly.  Does anyone have any
suggestions?



Thank you in advance.



Robin



Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com



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[Histonet] Dictaphone Troubles are over

[Parker, Helayne]

Thanks all- we got it fixed and it works like new now.

Thanks,

Helayne Parker, H.T. (ASCP)
Pathology Section Head

Skaggs Regional Medical Center
The Best Place to Get Better

P.O. Box 650, Branson Missouri 65615
Direct: 417-335-7254
Fax: 417-335-7127
E-Mail: hpar...@skaggs.net
Web: www.skaggs.net
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[Histonet] Full Time Position in Long Beach Calif.

[SHANE NELSON]

Hi All HT/HTL

Gastroenterology Group opening New Histology Lab in Long Beach Calif. Lab to be 
completed approximately in August. Candidate to have 3-5 years experience, self 
motivated and able to work well independently. Flexible hours, great pay. For 
more detailed information please contact me at information below.


THANK YOU,
 
PATTI RUBEN-NELSON  H.T.(ASCP) 
PNP LABORATORY CONSULTANT
SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonr...@verizon.net
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[Histonet] RE: Dictaphone System - HELP !!

[Rathborne, Toni]

We use the old Dicatphones too. Our service is provided from Nuance 
Communications. You can reach them at 866-383-9031.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne
Sent: Monday, May 16, 2011 1:24 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Dictaphone System - HELP !!


Hi All,
  Does anybody use the old Dictaphones ?  We have been using them and the 
company does not support them any longer.  The one at our grossing station went 
down Friday and was replaced by a newer one (as we had purchased several from a 
hospital that was getting out of them).  This one did not have a foot pedal so 
a foot pedal connection was adapted to it; however, when the other machine went 
down it was unplugged by another team member and all the programming was 
therefore lost.  Does anyone have Dictaphones that are operated by means of a 
foot pedal who's IT team would be willing to share the proper method to program 
in a foot pedal to use w/ it.  It is almost impossible to gross without a foot 
pedal. Thanks in advance.

Sincerely,
Helayne Parker, HT (ASCP)   
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or attached to this transmission is STRICTLY PROHIBITED. If you have received 
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or return e-mail and delete the original transmission and its attachments 
without reading or saving in any manner. Thank you.


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RE: [Histonet] sections falling off slides

[Liz Chlipala]

Robin

We run quite a few IHC's on goat bone femurs.  We use a very good plus
slide. Dry flat at 40C overnight and let them sit a few days before we
stain, we do not place them in a 60C oven since we find that the
articular cartilage may flip.  We have good success with proteinase K
with staining on the dako stainer for several antibodies.  If we have to
run HIER we use a 70C retrieval for 2 hours and then may choose to
manually stain depending upon the samples rather then placing on the
dako autostainer.  We have done this type of staining on porcine femurs
on 2x3 slides manually.  

I really think tissue adherence is dependent upon good fixation, optimal
decalcification and proper processing, if samples have been
insufficiently decaled or processed you are going to run into problems
with tissue adherence.  I can't comment to the lack of staining
intensity we always pilot a few slides prior to staining the entire
study just to make sure our methods will work for the particular tissues
we are currently working on.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin
Dean
Sent: Monday, May 16, 2011 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] sections falling off slides

Hi All,

 

We are trying to do IHC for multiple antigens on goat knees. The
sections
are decalcified FFPE sections and we are using a Dako autostainer. We
are
currently trying air-drying for  1-2 days  and baking of tissue slides
before use in IHC..

The one previous time we tried baking, we noticed a decrease in IHC
signal.
The sections are pretty large, we tried cutting them down, but can't
decrease the size much.  We also  decreased the number of washes and
size of
washes on the Daok, which helped only slightly.  Does anyone have any
suggestions?

 

Thank you in advance.

 

Robin

 

Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com

 

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[Histonet] sections falling off slides

[Robin Dean]

Hi All,

 

We are trying to do IHC for multiple antigens on goat knees. The sections
are decalcified FFPE sections and we are using a Dako autostainer. We are
currently trying air-drying for  1-2 days  and baking of tissue slides
before use in IHC..

The one previous time we tried baking, we noticed a decrease in IHC signal.
The sections are pretty large, we tried cutting them down, but can't
decrease the size much.  We also  decreased the number of washes and size of
washes on the Daok, which helped only slightly.  Does anyone have any
suggestions?

 

Thank you in advance.

 

Robin

 

Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com

 

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RE: [Histonet] uric acid crystals in tissue for gout

[Laurie Colbert]

We receive these specimens fresh and hand-process through 100% alcohol,
xylene, and paraffin.  The crystals are water soluble, so you need to
avoid any aqueous solutions. We cut one slide for routine H&E staining
and one slide that we just deparaffinize in xylene and then coverslip
directly out of that xylene.  The pathologist looks at the unstained
slide with a polarizing lens.  If the tissue gets processed routinely
(through formalin and the graded alcohols), we still cut the two slides
and sometimes the crystals are still there.
Laurie Colbert 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana
McCaig
Sent: Monday, May 16, 2011 5:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] uric acid crystals in tissue for gout

Does anyone have a method for identifying uric acid crystals in gout on
a tissue sample.

 

Diana

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[Histonet] HT/ HTL Position Columbia, SC

[Konni Black]

Hi All,

There is a fulltime opening for an experienced HT or HTL in Columbia, SC. 
Construction of this beautiful new lab will be completed by July. The ideal 
candidate will have 3 to 5 years experience and be able to work independently. 
They offer great pay and benefits and a very flexible scheduleno need to 
work at 4AM unless that is what YOU want! Interested parties may call @ 
253-503-2560 or e-mail me directly kbl...@digestivehlth.com. 

Thank you,
Konni Black
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[Histonet] Dictaphone System - HELP !!

[Parker, Helayne]

Hi All,
  Does anybody use the old Dictaphones ?  We have been using them and the 
company does not support them any longer.  The one at our grossing station went 
down Friday and was replaced by a newer one (as we had purchased several from a 
hospital that was getting out of them).  This one did not have a foot pedal so 
a foot pedal connection was adapted to it; however, when the other machine went 
down it was unplugged by another team member and all the programming was 
therefore lost.  Does anyone have Dictaphones that are operated by means of a 
foot pedal who's IT team would be willing to share the proper method to program 
in a foot pedal to use w/ it.  It is almost impossible to gross without a foot 
pedal. Thanks in advance.

Sincerely,
Helayne Parker, HT (ASCP)   
CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or 
previous e-mail messages attached to it may contain information that is 
confidential or legally privileged. If you are not the intended recipient, or a 
person responsible for delivering it to the intended recipient, you are hereby 
notified that you must not read this transmission and that any disclosure, 
copying, printing, distribution or use of any of the information contained in 
or attached to this transmission is STRICTLY PROHIBITED. If you have received 
this transmission in error, please immediately notify the sender by telephone 
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[Histonet] Re: uric acid crystals in tissue for gout

[Robert Richmond]

To see uric acid (actually monosodium urate) crystals in tissue, the
best thing to do is pick them out of the wet tissue with a needle, put
them on a slide in a drop of water with a coverslip, and examine them
promptly with polarization.

If they can't be seen on gross examination, then the special
processing techniques mentioned certainly help, though often MSU will
come through in routine processing.

The real problem is for the pathologist. Most of the small-hospital
pathologists I work with aren't allowed to have a polarizer on their
microscopes, so they have to take the trouble to go to the urine scope
(no, the broken-sunglasses shtick doesn't work), if they're allowed in
the urine lab which they sometimes aren't. And if they're willing to
take the time.

To identify MSU, you need to demonstrate negative birefringence. That
requires a full wave plate system (sometimes called a first order
plate), and somebody has to have preserved the documentation so that
the microscopist knows which way is up. In the absence of a procedure
manual, the pee scope can be quite frustrating. (Also I have to spend
five minutes cleaning and aligning it.)

I'm not telling you anything I don't have to put up with at least once a month.

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] Job Opening in Dallas, Texas

[Doug Showers]

ProPath, a progressive, CAP accredited, high volume, pathology practice,
located in Dallas, Texas, is seeking a Histotechnologist.  The hours for this
position are Monday through Friday, 2:00 pm to 10:30 pm.  In this position
you will be responsible for embedding tissue specimens, microtomy of
paraffin-embedded tissue, operation of automated stainers and coverslippers,
equipment maintenance and record retention.



The ideal candidate will have a high school diploma or equivalent.  We
prefer, HT, HTL (ASCP) registered or eligible.



Benefits include medical, dental, Short and Long Term Disability insurance, a
matched 401K plan and more!



Don't Follow the Leader!  Join the Leader!



EOE



For consideration send resume to:

ProPath, Human Resources

1355 River Bend Drive

Dallas, TX 75247

FAX: 214/237-1825

Job-Line: 214/237-1775

Email address: j...@propath.com

Website: www.propath.com





Doug Showers, MS, HT

Histology Manager

ProPath

1355 River Bend

Dallas, TX 75247



To learn more about ProPath, please visit http://www.ProPathLab.com









The information contained in this message may be privileged and confidential. 
If you are NOT the intended recipient, please notify the sender immediately 
with a copy to postmas...@propath.com and destroy this message.
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[Histonet] Amyloid stain

[Roberta Horner]

Does anyone have a procedure to do an amyloid stain on a smear?  Someone at the 
university wants to see if there is amyloid in milk.
Roberta Horner HT/HTl
Animal Diagnostic Lab
Penn State University
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Re: [Histonet] uric acid crystals in tissue for gout

[Patrick Laurie]

Here we just process starting in 70% EtOH, cut the section on a bath
of 70% EtOH (watch out, it wrinkles very bad) and stain them in an
Eosin only stain (which is the H&E with all of the aqueous parts taken
out).  The pathologist looks at them with a polarizing filter.  Make
sure that they stay away from water!
A good article that talks about this is:
Evaluation of Crystals in Formalin-Fixed, Paraffin-Embedded Tissue
Sections for the Differential Diagnosis of Pseudogout, Gout, and
Tumoral Calcinosis
Shidham et. al. Mod Pathol 2001;14(8):806–810
Good luck!

On Mon, May 16, 2011 at 5:46 AM, Diana McCaig  wrote:
> Does anyone have a method for identifying uric acid crystals in gout on
> a tissue sample.
>
>
>
> Diana
>
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plau...@cellnetix.com

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[Histonet] Microtome Quote

[Lin Bustamante]

I am so sorry I misplaced the hard copy I made of a quote I need and my
computer crashed with a storm we had last week.
Someone sent me a quote for 4 Leica/Reichert 2030 rotary microtomes and
2 or 4 water bath.
The price for each microtome was $3,200 (I believe) and the water bath
less than $700.00 each.
Please send me the quote again.
Thank you and I apologise again.
Lin Bustamante

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas A&M University
College Station, TX 77843-4458
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[Histonet] Field Support Opportunity with a World Leader

[Matt Ward]

Good Morning Histonet!



One of our retained Cancer Diagnostics clients has opened up an expansion
Field Technical Support Opportunity perfect for a Histotech looking to get
out of the lab and into the field. Please send your resumes to
m...@personifysearch.com, or call me at 800.875.6188 ext. 103 so I can answer
any questions.



Also, if you know of anyone who may be interested please do not hesitate to
forward this e-mail, and have them reach out to me.



Have a great day!



Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com



*Field Support Specialist*


*The Company:*

Our client is a leading developer and producer of innovative high-tech
precision optics systems for the analysis of microstructures.  As one of the
market leaders in each of the fields of Microscopy, Confocal Laser Scanning
Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment.
Comprising nine manufacturing facilities in seven countries, sales and
service companies in 20 countries and an international network of dealers,
the company is represented in over 100 countries.

*The Opportunity:*

The company currently has an opening for a Field Support Specialist in
Cancer Diagnostics to cover the Mid Atlantic Region and to be based in
Baltimore or Washington DC.  All applicants must not be adverse to travel,
as this is a position that may require you to travel when necessary.

Base: $60-65k per year + bonus/commission

Car Allowance: $700/month + Gas Card

Cell Phone/Laptop

Other: Full benefits - 401k program/matching

*Primary Responsibilities: *

The primary responsibility of this role will be to provide in-field
technical applications support for current and next generation range of
products.  The FSS will install/set-up instrumentation in customer
laboratories, perform demonstrations and maintain demonstration equipment in
a clean and operational manner.  The FSS will also train customers on the
use of instrumentation.  Conducting in-field post purchase applications
support and performing instrument validations will be a key responsibility
of this position.

Additional Responsibilities:
- Build an in-depth understanding of new product technologies and their
applications in a diagnostic environment
- Contribute to the strategic planning process by providing technical data
on instrumentation and reagents
- Conduct post-purchase reagent order management
- Be the customer liaison by providing scientific and laboratory expertise
for in-field installations, evaluations, training and trouble-shooting
- Provide customer support for remote problem solving
- Design and perform experiments to investigate and solve tough technical
applications problems
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[Histonet] uric acid crystals in tissue for gout

[Diana McCaig]

Does anyone have a method for identifying uric acid crystals in gout on
a tissue sample.

 

Diana

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[Histonet] In need of HT/HTL in Little Rock, AR

[Pathology Partners]

Pathology Partners in Little Rock, Arkansas is in need of an experienced HT or 
HTL to fill a full time position.  

Position requirements:  Certified HT or HTL
   Must meet CLIA/CAP guidelines for tissue 
grossing
   Must be able to demonstrate expertise in 
histology, including the following:  grossing GI specimens, embedding, 
microtomy, IHC and special staining of GI 

tissue.
 
Shift: M-F 9:00am - 6:00 pm
Rate of pay is hourly and negotiable based on experience.
Full benefits including vision, dental and health available.
90 day probationary period.

Please fax all resumes to 501-687-9228 or email to 
histo...@pathology-partners.com
Call 501-687-9220 and ask for Clay.

Sincerely,

Clay Milks 

Histology Laboratory

Pathology Partners LLC

histo...@pathology-partners.com

 

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is prohibited.  If you are not the intended recipient, please contact the 
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