[Histonet] Library-Freida Carson

[SHANE NELSON]

Mrs. Carson,
Would it be possible to e-mail me the cost of these books? I would greatly 
appreciate it and I am very interested.



Carson:  Histotechnology: A Self-Instructional Text - 2nd edition.
2007

Bancroft and Cook: Manual of Histological Techniques and their Diagnostic 
Application. 1994


Wheater, Burkitt and Daniels: Functional Histology. 1987.

Lillie and Fullmer: Histopathologic Technic and Practical Histochemistry. 1976

Vacca: Laboratory Manual of Histochemistry.  1985

Bancroft and Stevens: Theory and Practice of Histological Techniques. 1996


THANK YOU,
 
PATTI RUBEN-NELSON  H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonr...@verizon.net
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Re: [Histonet] (no subject)

[Rene J Buesa]

Validating an Ab does not involve testing different concentrations, just a 
known (+) tissue to be evaluated by the pathologist that should compare the 
results with other tissues already reacted with the same Ab and protocol.
If the pathologist accepts the results, you have your validation.
René J.

From: Dorothy Glass 
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, June 15, 2011 11:41 AM
Subject: [Histonet] (no subject)

Dear Histonets, 
How is it possible to validate a antibody on the Ventana Ultra or XT, when the 
antibodys afre prediluted or made into a prep-kit?
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[Histonet] Downsizing my library

[Freida Carson]

I am downsizing my library and have the following books, mostly out of print, 
for sale.  If you are interested, make an offerf or any of them with enough to 
cover postage also. I hate to just throw them away. Please do this to my 
private email and not on histonet.

Carson:  Histotechnology: A Self-Instructional Text - 2nd edition.
2007
Bancroft and Cook: Manual of Histological Techniques and their Diagnostic 
Application. 1994

Montgomery: Health and Safety Guidelines for the Laboratory, 1995.

Dapson and Dapson: Hazardous Materials in the Histopathology Laboratory. 3rd 
ed, 1995

Wheater, Burkitt and Daniels: Functional Histology. 1987.

Lillie and Fullmer: Histopathologic Technic and Practical Histochemistry. 1976

Kiernan: Histological & Histochemical Methods. 1999.

Vacca: Laboratory Manual of Histochemistry.  1985

Bancroft and Stevens: Theory and Practice of Histological Techniques. 1996


Freida Carson 

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[Histonet] RE: Source for p16 antibody...

[McMahon, Loralee A]

MTM labs is the only place to get it IVD. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar 
[tmcne...@lmhealth.org]
Sent: Wednesday, June 15, 2011 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Source for p16 antibody...

Hello all,

I'm looking for p16 antibody for use on the Benchmark XT and was wondering what 
others are using and where they are getting it.  Any input appreciated.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org



This e-mail, including attachments, is intended for the sole use of the 
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Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
accept liability for any errors or omissions in the contents of this message, 
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Re: [Histonet] (no subject)

[histot...@imagesbyhopper.com]

Dorothy,

Is your question related to the fact that a protocol will have the "currently 
active" lot number and you want to use a different lot number?  Or is this a 
brand new antibody that has not been in use before and therefore has not ever 
been validated?

Michelle


On Jun 15, 2011, at 11:41 AM, Dorothy Glass  wrote:

> Dear Histonets, 
> How is it possible to validate a antibody on the Ventana Ultra or XT, when 
> the 
> antibodys afre prediluted or made into a prep-kit?
> ___
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[Histonet] RE: Source for p16 antibody...

[Walter Benton]

 MTM is the only company that can sell the antibody.
http://www.mtmlabs.com/us/index.php

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar 
[tmcne...@lmhealth.org]
Sent: Wednesday, June 15, 2011 1:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Source for p16 antibody...

Hello all,

I'm looking for p16 antibody for use on the Benchmark XT and was wondering what 
others are using and where they are getting it.  Any input appreciated.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org



This e-mail, including attachments, is intended for the sole use of the 
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error-free as information could be intercepted, corrupted, lost, destroyed, 
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RE: [Histonet] RE: Retirement

[Ingles Claire]

Try about 30 (years)!
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda
Sent: Tue 6/14/2011 2:25 PM
To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Retirement



7 years compared to 8 months is gigantically significant.





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[Histonet] Source for p16 antibody...

[Tom McNemar]

Hello all,

I'm looking for p16 antibody for use on the Benchmark XT and was wondering what 
others are using and where they are getting it.  Any input appreciated.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org



This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
accept liability for any errors or omissions in the contents of this message, 
which arise as a result of e-mail transmission. Thank you.
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RE: [Histonet] (no subject)

[Liz Chlipala]

Dorothy

I would assume that you would just run the validation as you would any
other antibody that is not a pre dilute.  Run the antibody protocol on
the number of tissues that you normally do to validate, evaluate and
record the results.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy
Glass
Sent: Wednesday, June 15, 2011 9:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Dear Histonets, 
How is it possible to validate a antibody on the Ventana Ultra or XT,
when the 
antibodys afre prediluted or made into a prep-kit?
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[Histonet] (no subject)

[Dorothy Glass]

Dear Histonets, 
How is it possible to validate a antibody on the Ventana Ultra or XT, when the 
antibodys afre prediluted or made into a prep-kit?
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RE: [Histonet] RE: Histonet Digest, Vol 91, Issue 21; Keeping Histo room floor clean? (JR R)

[Turner, Leandra]

Ohh and we also got housekeeping to give us a broom and long handled
dust pan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Turner,
Leandra
Sent: Wednesday, June 15, 2011 10:53 AM
To: Mayer,Toysha N; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 91, Issue 21;Keeping
Histo room floor clean? (JR R)

Hi,
We use sticky floor mats from market lab that help to collect any stray
paraffin.  We use a scraper from market lab, it has a long handle so you
don't have to stoop over, to scrap up any paraffin that did not land on
the maps.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Mayer,Toysha N
Sent: Wednesday, June 15, 2011 10:17 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 21; Keeping Histo
room floor clean? (JR R)

I will call myself old school and say, a slide works well and can be
disposed of in the broken glass box.
Now you can get your housekeeping dept to just shower scrub your floor
and not put wax on it.  But the putty knife, especially on a broom or
mop handle is great, and easier on the knees and back.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnma...@mdanderson.org

Message: 15
Date: Tue, 14 Jun 2011 20:49:57 -0400
From: "histot...@imagesbyhopper.com" 
Subject: Re: [Histonet] Keeping Histo room floor clean?
To: JR R 
Cc: ""

Message-ID: <54b2b63a-3054-414a-9d4c-97241b250...@imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii

We don't keep it off the floor, but do use a wide-bladed putty knife
attached to a mop handle to scrape the residual wax off the floor. It
woks quite nicely and doesn't remove the actual floor wax like a razor
blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo
room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the
floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
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RE: [Histonet] RE: Histonet Digest, Vol 91, Issue 21; Keeping Histo room floor clean? (JR R)

[Turner, Leandra]

Hi,
We use sticky floor mats from market lab that help to collect any stray
paraffin.  We use a scraper from market lab, it has a long handle so you
don't have to stoop over, to scrap up any paraffin that did not land on
the maps.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Mayer,Toysha N
Sent: Wednesday, June 15, 2011 10:17 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 91, Issue 21; Keeping Histo
room floor clean? (JR R)

I will call myself old school and say, a slide works well and can be
disposed of in the broken glass box.
Now you can get your housekeeping dept to just shower scrub your floor
and not put wax on it.  But the putty knife, especially on a broom or
mop handle is great, and easier on the knees and back.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnma...@mdanderson.org

Message: 15
Date: Tue, 14 Jun 2011 20:49:57 -0400
From: "histot...@imagesbyhopper.com" 
Subject: Re: [Histonet] Keeping Histo room floor clean?
To: JR R 
Cc: ""

Message-ID: <54b2b63a-3054-414a-9d4c-97241b250...@imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii

We don't keep it off the floor, but do use a wide-bladed putty knife
attached to a mop handle to scrape the residual wax off the floor. It
woks quite nicely and doesn't remove the actual floor wax like a razor
blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo
room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the
floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
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[Histonet] P5049-15 - Anyone interested buying some Pipette tips for Oxford pipetters

[Brian Branton]

Hello HistoNetters,

 

I was cleaning out a cabinet in the Laboratory, and came across 8 bags
of Allegiance S/P AccuTip Nonfiltered Pipett Tips (# P5049-15). They fit
a Oxford P-3000 or P-7000 pipetter. We no longer have the pipetter for
these tips. I am selling all eight bags for $20.00 plus shipping.

 

Would anyone be interested?

 

Brian Branton 
Purchasing Agent 

SaraPath Diagnostics

Sarasota Pathology

Sarasota Professional Enterprises II 
(941) 362-8963 
(941) 362-8964 FAX 

 

 

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[Histonet] RE: Histonet Digest, Vol 91, Issue 21; Keeping Histo room floor clean? (JR R)

[Mayer,Toysha N]

I will call myself old school and say, a slide works well and can be disposed 
of in the broken glass box.
Now you can get your housekeeping dept to just shower scrub your floor and not 
put wax on it.  But the putty knife, especially on a broom or mop handle is 
great, and easier on the knees and back.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnma...@mdanderson.org

Message: 15
Date: Tue, 14 Jun 2011 20:49:57 -0400
From: "histot...@imagesbyhopper.com" 
Subject: Re: [Histonet] Keeping Histo room floor clean?
To: JR R 
Cc: ""

Message-ID: <54b2b63a-3054-414a-9d4c-97241b250...@imagesbyhopper.com>
Content-Type: text/plain; charset=us-ascii

We don't keep it off the floor, but do use a wide-bladed putty knife attached 
to a mop handle to scrape the residual wax off the floor. It woks quite nicely 
and doesn't remove the actual floor wax like a razor blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo room--especially 
> around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


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confidential information that is protected by law and is for the
sole use of the individuals or entities to which it is addressed.
If you are not the intended recipient, please notify the sender by
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communication and attachments. Further use, disclosure, copying,
distribution of, or reliance upon the contents of this email and
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Re: [Histonet] decal fish larvae

[Rene J Buesa]

Try EDTA for at least 24 hours
René J.

From: Tora Bardal 
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, June 15, 2011 5:16 AM
Subject: [Histonet] decal fish larvae

Hi

Does anyone have a suggestion for decal solution/ time for (weakly) ossified 
bone in fish larvae?  Paraffin section tends to split due to the harder tissue, 
nice longitudinal section are hard to get.



Tora Bardal
Overingeniør/ Senior Engineer
NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture
NTNU, 7491 Trondheim


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[Histonet] special stain validation documentation

[Jeff Birkner]

Just wondering how everyone is formally documenting validation of manual
special stains.  If you have a template/form to share that would be
great.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)

Manager, Pathology Laboratory Section

Collaborative Laboratory Services, L.L.C.

1005 Pennsylvania Ave, Suite 102

Ottumwa, IA  52501

641-455-5414

ORHC Extension #3538

jbirk...@colabserv.com

 

 

 

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Re: [Histonet] Keeping Histo room floor clean?

[Grantham, Andrea L - (algranth)]

I periodically use a scraper to get the wax off the floor under the microtomes 
and I have housekeeping strip the floors a couple of times a year. We also use 
a shop vac to vacuum up around the embedder and throughout the lab. We have 
sticky mats at the entrance to the microtome stations and at the door to the 
embedding room. Also Market lab makes these mats that trap paraffin and we have 
them throughout the lab. They come in a couple different sizes and also you can 
get custom sizes.
All this because we almost took out our department head one day when he went 
slipping over the waxy floor - before my time but I imagine it wasn't pretty.
You also just have to be aware that what we do produces waxy debris and you 
have to be careful as to not make such a big mess. I teach the students to be 
neat and to clean up after themselves - and they do!

Andi


On Jun 14, 2011, at 3:44 PM, JR R wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo room--especially 
> around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
> 
> ___
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RE: [Histonet] Paraffin processing and embeding of Chondrocyte pellets.

[Liz Chlipala]

Bret

We have done a bit of work with these types of samples, the first thing
we do is remove the formalin and add eosin to the container for about 5
minutes.  Then we place the pellet (using a plastic disposable pipettor)
in a mesh bag and process on our tissue processor on a cycle that is set
at 7 minutes per station, we also used propar instead of xylene (3
changes).  We used to process by hand but this method works just as
well.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough,
Bret
Sent: Tuesday, June 14, 2011 8:52 PM
To: Histonet list serv. (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte
pellets.

Hello Everyone,
 I was wondering if anyone has a protocol for paraffin embedding
chondrocyte pellets that they would be willing to share with me? Any
help would be greatly appreciated.

Thanks,
 Bret

Bret Clough
TAMHSC

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[Histonet] RE: Paraffin processing and embeding of Chondrocyte

[Linn, Marisa]

Hi Bret-
I've dealt with chondrocyte pellets a few times.  It's a pretty half-assed 
technique, but works pretty well if the pellet is relatively solid...if it's 
not, maybe someone else can offer a useful suggestion :)
I was taught to use a piece of lens paper; pre-fold it in half several times, 
and drop the pellet along one of the folds and close the thing up.  If you trap 
an edge or two of the paper in the cassette lid (or put a sponge in), the paper 
shouldn't be able to unfold.  When the time comes to embed, keep that paraffin 
nice and melty.  
For sectioning...check frequently under a scope.   
Good luck,
Marisa

-Original Message-

Message: 19
Date: Wed, 15 Jun 2011 02:52:29 +
From: "Clough, Bret" 
Subject: [Histonet] Paraffin processing and embeding of Chondrocyte
pellets.
To: "Histonet list serv. (histonet@lists.utsouthwestern.edu)"

Message-ID:

Content-Type: text/plain; charset="iso-8859-1"

Hello Everyone,
 I was wondering if anyone has a protocol for paraffin embedding chondrocyte 
pellets that they would be willing to share with me? Any help would be greatly 
appreciated.

Thanks,
 Bret

Bret Clough
TAMHSC

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[Histonet] histotechnologist open position

[Barbara.Crill]

Danville Regional Medical Center located in Danville, VA has an opening for a 
histotechnologist.
You may apply on line:
http://lifepointhealthcontent.com/custompage.asp?PageName=danvilletaleo

For Further information please contact our human resources department
434.799.4552 or 434.799.3719


Job Description
Histologist-112870 Danville Regional Medical Center
Description

Performs routine and assigned histology and pathology functions.  Processes, 
prepared, cuts, mounts, and stains tissue specimens from patients of all age 
groups, including adults, adolescents, pediatrics and infants to provide high 
quality anatomic pathology services in a cost efficient manner.
Qualifications

Baccalaureate degree AND/OR completion of technical training school in 
Histology.  HTL (ASCP) or eligible
Job
 Laboratory
Primary Location
 Virginia-Danville

Schedule
 Full-time
Shift
 Day Job



ANTOINETTE CRILL, MBA,CT(ASCP)
TEAM LEADER ANATOMIC PATHOLOGY
DANVILLE REGIONAL MEDICAL CENTER
(O) 434.799.4470
(F) 434.773.6806
E-mail:  barbara.cr...@lpnt.net

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RE: [Histonet] Keeping Histo room floor clean?

[DANIEL HEWITT]

We us a scraper I bought at Home Depot called the floor buster.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of CHRISTIE
GOWAN
Sent: Wednesday, June 15, 2011 10:21 AM
To: sgoe...@mirnarx.com; histot...@imagesbyhopper.com;
rosenfeld...@hotmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Keeping Histo room floor clean?


We use a razor blade scraper but we turn it around and use the dull
side.
 

> Date: Wed, 15 Jun 2011 08:59:21 -0500
> From: sgoe...@mirnarx.com
> To: histot...@imagesbyhopper.com; rosenfeld...@hotmail.com
> Subject: RE: [Histonet] Keeping Histo room floor clean?
> CC: histonet@lists.utsouthwestern.edu
> 
> You can buy this type of thing too if you aren't the McGiver type. For
> instance...from American Mastertech item # CPW04200E
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> histot...@imagesbyhopper.com
> Sent: Tuesday, June 14, 2011 7:50 PM
> To: JR R
> Cc: 
> Subject: Re: [Histonet] Keeping Histo room floor clean?
> 
> We don't keep it off the floor, but do use a wide-bladed putty knife
> attached to a mop handle to scrape the residual wax off the floor. It
> woks quite nicely and doesn't remove the actual floor wax like a razor
> blade scrapper would.
> 
> Michelle
> 
> Sent from my iPhone
> 
> On Jun 14, 2011, at 6:44 PM, JR R  wrote:
> 
> > 
> > We keep getting a lot of paraffin on the floor of one histo
> room--especially around the microtome and the embedding station.
> > 
> > Short of laying down a tarp, what do folks do keep wax off of the
> floor?
> > 
> > Thanks,
> > 
> > Jerry Ricks
> > Research Scientist
> > University of Washington
> > Department of Pathology
> >
> ___
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> 
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RE: [Histonet] Keeping Histo room floor clean?

[CHRISTIE GOWAN]

We use a razor blade scraper but we turn it around and use the dull side.
 

> Date: Wed, 15 Jun 2011 08:59:21 -0500
> From: sgoe...@mirnarx.com
> To: histot...@imagesbyhopper.com; rosenfeld...@hotmail.com
> Subject: RE: [Histonet] Keeping Histo room floor clean?
> CC: histonet@lists.utsouthwestern.edu
> 
> You can buy this type of thing too if you aren't the McGiver type. For
> instance...from American Mastertech item # CPW04200E
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> histot...@imagesbyhopper.com
> Sent: Tuesday, June 14, 2011 7:50 PM
> To: JR R
> Cc: 
> Subject: Re: [Histonet] Keeping Histo room floor clean?
> 
> We don't keep it off the floor, but do use a wide-bladed putty knife
> attached to a mop handle to scrape the residual wax off the floor. It
> woks quite nicely and doesn't remove the actual floor wax like a razor
> blade scrapper would.
> 
> Michelle
> 
> Sent from my iPhone
> 
> On Jun 14, 2011, at 6:44 PM, JR R  wrote:
> 
> > 
> > We keep getting a lot of paraffin on the floor of one histo
> room--especially around the microtome and the embedding station.
> > 
> > Short of laying down a tarp, what do folks do keep wax off of the
> floor?
> > 
> > Thanks,
> > 
> > Jerry Ricks
> > Research Scientist
> > University of Washington
> > Department of Pathology
> >
> ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> 
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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RE: [Histonet] Keeping Histo room floor clean?

[sgoebel]

You can buy this type of thing too if you aren't the McGiver type.  For
instance...from American Mastertech item # CPW04200E

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Tuesday, June 14, 2011 7:50 PM
To: JR R
Cc: 
Subject: Re: [Histonet] Keeping Histo room floor clean?

We don't keep it off the floor, but do use a wide-bladed putty knife
attached to a mop handle to scrape the residual wax off the floor. It
woks quite nicely and doesn't remove the actual floor wax like a razor
blade scrapper would.

Michelle

Sent from my iPhone

On Jun 14, 2011, at 6:44 PM, JR R  wrote:

> 
> We keep getting a lot of paraffin on the floor of one histo
room--especially around the microtome and the embedding station.
> 
> Short of laying down a tarp, what do folks do keep wax off of the
floor?
> 
> Thanks,
> 
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
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[Histonet] mouse-on-mouse kit and non-specific staining

[iris oren]

Hi, 

I am using a mouse monoclonal antibody on PFA-fixed mouse brain slices, and the 
Vector mouse-on-mouse kit. My background with the provided avidin-biotyn is 
ridiculously high even after blocking endogenous biotyn. However, I get an 
overall low background using an Alexa-conjugated goat anti-mouse (with the 
slices pre-treated in Normal Goat Serum). The problem is that in the control 
sections with no primary, I still get staining of isolated cells.
Does anyone have any thoughts/experience/recommendation with using an Alexa 
conjugated anti mouse IgG secondary on mouse tissue (with a mouse-on-mouse 
block)?

Many thanks in advance
Iris

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RE: [Histonet] decal fish larvae

[Debbie Faichney]

Hello Tora,

We surface decal the trimmed block, for one hour, using a commercial product 
called Rapid Decalcifier (Cellpath, UK). I don't know what it contains, other 
than Hydrochloric acid, but it works a treat.  Other alternatives for fish have 
been Nair hair removal cream (applied to the surface for 10 mins or so) and 
fabric softner (dilute 1:10 in distilled water and soak for 30 min to an hour)
Hope you are successful

Debbie Faichney
Institute of Aquaculture
University of Stirling
Scotland

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tora Bardal
Sent: 15 June 2011 10:16
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decal fish larvae

Hi

Does anyone have a suggestion for decal solution/ time for (weakly) 
ossified bone in fish larvae?  Paraffin section tends to split due to 
the harder tissue, nice longitudinal section are hard to get.



Tora Bardal
Overingeniør/ Senior Engineer
NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture
NTNU, 7491 Trondheim


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-- 
The Sunday Times Scottish University of the Year 2009/2010
The University of Stirling is a charity registered in Scotland, 
 number SC 011159.


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[Histonet] Thank You

[Gauch, Vicki]

Thank you to everyone who responded to my decal solution for IHC 
questionyour help was greatly appreciated...
Have a great day,
Vicki Gauch
AMCH
Albany, NY



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[Histonet] decal fish larvae

[Tora Bardal]

Hi

Does anyone have a suggestion for decal solution/ time for (weakly) 
ossified bone in fish larvae?  Paraffin section tends to split due to 
the harder tissue, nice longitudinal section are hard to get.




Tora Bardal
Overingeniør/ Senior Engineer
NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture
NTNU, 7491 Trondheim


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