[Histonet] Fluorescent Label for Collagen-1
Dear Histonetters, I'm performing fluorescent microscopy on PFA-fixed, paraffin-embedded rat tissue. I need to stain Collagen with a fluorescent label. I was trying a goat-anti-hu/ms/rat-Col1A Ab from Santa Cruz (sc-25974) but I simply can't see a signal. My question would be whether one of you can recommend either an antibody which actually works for rat tissue or an alternative fluorescent probe I could use. I recently read about the Collagen-binding protein CNA35, which looks very promising, but I don't have the means of labeling this protein for myself and I couldn't find a vendor for a labeled version of CNA35. I'd be glad to hear your thoughts and ideas on this topic! All the best, Till ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Number of blocks before a processor change???
We rotate one of each solution type(including wax) every 50 blocks or every 2 runs(whichever occurs first). Helps counteract carryover from sponges and seems to work better and conserve a little on reagents than when doing complete change less frequently. Note: we rune a large number of small biopsies using sponges. Pat Valente Histo Manager Healthtronics San Antonio From: Sheila Adey To: histonet@lists.utsouthwestern.edu Sent: Sat, July 9, 2011 8:31:52 AM Subject: [Histonet] Number of blocks before a processor change??? Hello: We currently change our processors every 5 runs. I am thinking it would be more cost and time effective to count the number of blocks before doing a change. If you count your blocks could you share with me at what number you do a change. We run all types of tissues. We use a gallon of reagent in each position. Vip5. Thanks in advance. :) Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Many thanks: radionuclides query
Hi All, Sincere thanks to all who responded to my query on tissue containing radionuclides and CAP - especially Galbraith Joe, Connolly Bret and Dawn. Apologies if I missed out anyone else. Joyce Fortin: I have forwarded all of these replies to you. Best Wishes Shehnaz Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Number of blocks before a processor change???
I always counted the number of blocks processed per run. When my VIP reached its maximum (e.g. 300 blocks), I changed all the alcohols of less than 100%, and moved "down" the 100EthOL, the xylenes and the paraffins (meaning eliminating the first 100 EthOL, xylene and paraffin, and replacing the last). René J. --- On Sat, 7/9/11, Sheila Adey wrote: From: Sheila Adey Subject: [Histonet] Number of blocks before a processor change??? To: histonet@lists.utsouthwestern.edu Date: Saturday, July 9, 2011, 9:31 AM Hello: We currently change our processors every 5 runs. I am thinking it would be more cost and time effective to count the number of blocks before doing a change. If you count your blocks could you share with me at what number you do a change. We run all types of tissues. We use a gallon of reagent in each position. Vip5. Thanks in advance. :) Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] prostate bx containers
Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Number of blocks before a processor change???
Hello: We currently change our processors every 5 runs. I am thinking it would be more cost and time effective to count the number of blocks before doing a change. If you count your blocks could you share with me at what number you do a change. We run all types of tissues. We use a gallon of reagent in each position. Vip5. Thanks in advance. :) Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
