[Histonet] HistoTALK at the NSH Convention

[David Kemler]

Hello HistoNetters -
 
I'll be presenting next month at the NSH Symposium/Convention AND producing a 
few HistoTALK shows. If you are also presenting a workshop and would like to 
talk about your workshop on HistoTALK, let me know and I'll schedule a 
convenient time to be interviewed. Ask anybody who has been on the show and 
they will tell you that it's a great time, lots of fun and easier than cutting 
your finger on a microtome blade! Check out www.HistoTALK.com to see other 
HistoPersons (Aubrey, Carrie, Nancy Heath, Elaine Basham, Beth Sheppard, Skip 
Brown, Lawerence Patton, Sheri Blair, Lacy Cardillo, Rose Tavares, etc; etc; 
etc;) who have taken the big step into internet radio!
 
If you are not presenting and have something of interest to pass on to the 
Histo-Community, let me know. We'll see about getting you scheduled as well.
 
Yours,
Dave
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[Histonet] IF antibodies for Human Lung Tissue

[Kathy Bonness]

 Hello-

   I will be IF staining human lung tissue, both cancerous (NSCLC) and 
benign, and I am looking for suggestions regarding primary antibodies that have 
been successful. To develop our model, I would prefer to use 'tried and true' 
antibodies rather than re-inventing the wheel with precious samples.



   Any suggestions would be greatly appreciated for either FFPE or frozen 
tissue samples as I will be using both methods for IF staining of patient 
samples. Thank you in advance for any suggestions or advice.



Cheers-

Kathy M. Bonness, PhD.



251-533-2661

kathy.bonn...@utsouthwestern.edu

http://www.linkedin.com/pub/kathy-bonness/6/1a1/931



UTSW  Dallas, TX

Green Center for Computational & Systems Biology

Department of Pharmacology

Altschuler/Wu Lab   (ND9.214)

http://www4.utsouthwestern.edu/altschulerwulab/



UT Southwestern Medical Center
The future of medicine, today.
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[Histonet] (no subject)

[mohamed abd el razik]

http://capelladunboycastle.com/oldtemp/vfst.htm";>http://capelladunboycastle.com/oldtemp/vfst.htm
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[Histonet] Re: Baboon Tissues

[Johnson, Teri]

Mike,

It sounds like a classic case of either slow freezing or thawing and slow 
re-freezing causing ice crystal formation. One other guess is that they were 
fixed in formalin but not cryoprotected, and then frozen. I have seen freezing 
artifact in this way as well. Unfortunately there is nothing you can do at this 
point to save the samples. They are architecturally ruined.

It is entirely possible to snap freeze unfixed tissues properly without a 
sucrose gradient. Matter of fact, we only use the sucrose cryoprotection step 
on samples that have been previously fixed. As for the proper collection of the 
new samples, that depends on what will need to happen to them after they are 
mounted on the slide. The best histology results from fixed and cryoprotected 
frozen section. You can usually get something that looks almost as good as a 
paraffin section. Arguably the best IHC is achieved on unfixed and snap frozen 
tissues. Gayle Callis is the master at doing this, she has worked in rodent 
spleen in cryo for many years and can give you tons of good advice on 
sectioning and staining them. You should do a histonet search looking for her 
information on sample handling if you indeed need unfixed frozen sections.

For fixed frozens, definitely put them through the sucrose gradient and then 
snap freeze. Make sure your sectioning temperature is not too cold (shattering 
of the tissue), or too warm (ooey gooey sticky mess). You are looking for the 
Goldilocks temp - just right.

Good luck!

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO


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[Histonet] RE: Histonet Digest, Vol 93, Issue 29

[Andy Hall]

The only part that is not completed is the last back brought in last week,
otherwise everything is completed so far

Andy Hall (HTL-ASCP)
RRC Histology Core
909 S. Wolcott Ave 
COMRB RM. 6100
Chicago, IL 60612
Ph:312-996-3869


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, August 23, 2011 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 93, Issue 29

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Today's Topics:

   1. Histotechnologist in IHC (Miriam)
   2. Re: Cassette Marking Pen (histot...@imagesbyhopper.com)
   3. Jennifer Thawley is out of the office.
  (jthaw...@shorememorial.org)
   4. control tissue for IHC, ffpe (Patsy Ruegg)
   5. CAP Requirement??? (histot...@imagesbyhopper.com)
   6. Histotechnologist Position-University Hospital UMDNJ
  (Delia, Catherine)
   7. p53 staining in mouse tissue (Sally Price)
   8. Re: (lynn13...@aol.com)
   9. slide box freezer racks (Ed Roy)
  10. CSF billing.  (Podawiltz, Thomas)
  11. Re: slide box freezer racks (Jan Shivers)
  12. about some antiboides.. (Mehmet Fatih BOZKURT)
  13. Nile Red stain on Froz. muscle bx's (Sharon Allen)
  14. RE: Nile Red stain on Froz. muscle bx's (Houston, Ronald)
  15. Baboon Tissues (Mike Tighe)
  16. Eosinophil Cationic Protein (Houston, Ronald)


--

Message: 1
Date: Mon, 22 Aug 2011 14:34:46 -0400 (EDT)
From: Miriam 
Subject: [Histonet] Histotechnologist in IHC
To: histonet@lists.utsouthwestern.edu
Message-ID: <8ce2f114d05f068-18cc-6...@webmail-d051.sysops.aol.com>
Content-Type: text/plain; charset="utf-8"


Dear Group Members,


I was hoping you might be able to recommend someone for the following
opportunity: 

Southeastern MA b


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[Histonet] Eosinophil Cationic Protein

[Houston, Ronald]

Does anyone know of a source of ECP for use  on human paraffin embedded 
material? Pharmacia used to carry it but since they were bought out it is only 
availble as part of an ELISA kit?
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster


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[Histonet] Baboon Tissues

[Mike Tighe]

Hi All,

We received some frozen Baboon spleen tissue from the NIH that turned out to be 
very difficult to cut. I am experienced with Mouse tissues but rarely have to 
cut tissue the size of my thumb. The best information I could get was that the 
tissue was not put through a sucrose gradient but put directly into OCT. The 
OCT seemed to come off the blade just fine but the tissue seemed spongy and 
looked more like lung tissue (with many holes) once it was warmed on a slide. 
My first thought was that it had been put through a sucrose gradient that was 
not complete. I tried colder and warmer temps with thicker and thinner sections.

My question list

Is there anybody who has had a similar problem and do you think it is a tissue 
processing problem (No hope).
Do you think it is a cutting problem? (only hope is for a better technician 
than me, maybe)

 I have an opportunity to get more tissues collected from a collaborator. What 
would be the best way to collect these tissues be?

Thanks again for all your help!!!
Mike









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[Histonet] RE: Nile Red stain on Froz. muscle bx's

[Houston, Ronald]

J Histochem Cytochem. 1987 May;35(5):619-21.
Application of nile blue and nile red, two fluorescent probes, for detection of 
lipid droplets in human skeletal muscle.
Bonilla E, Prelle A.
Abstract
Using frozen sections from human muscle biopsies, we assessed the value of Nile 
blue and Nile red, two fluorescent probes, as stains for lipid droplets in 
normal and pathological skeletal muscle fibers. In normal muscle, lipid storage 
disorders, and mitochondrial myopathies, Nile blue stained the lipid droplets 
as yellow-gold fluorescent structures. The lipid droplets were also seen as 
yellow-gold fluorescent structures in Nile red-stained sections, but the 
outstanding feature in these preparations was the staining of the membrane 
network of the muscle fibers and membrane proliferations in pathological muscle 
as red-orange fluorescent structures. These results suggest that both Nile blue 
and Nile red stains are useful for visualization of lipid droplets and membrane 
proliferations in pathological muscle biopsies.

PMID:3559182[PubMed - indexed for MEDLINE]



Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Allen
Sent: Tuesday, August 23, 2011 10:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nile Red stain on Froz. muscle bx's

Hi,
Does anyone have a method for staining frozen skeletal muscle bx's with Nile 
Red &/or Nile Blue for the detection of lipid droplets.
Our Neuropathologist saw the stain at a conference & would like us to try it. 
Thanks

Sharon Allen
Senior Technologist
Neuropathology Lab MS435U
Health Sciences Centre
825 Sherbrook St.
Winnipeg, Manitoba R3A 1R9
Ph# 787-4615


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[Histonet] Nile Red stain on Froz. muscle bx's

[Sharon Allen]

Hi,
Does anyone have a method for staining frozen skeletal muscle bx's with
Nile Red &/or Nile Blue for the detection of lipid droplets.
Our Neuropathologist saw the stain at a conference & would like us to
try it. 
Thanks

Sharon Allen
Senior Technologist
Neuropathology Lab MS435U
Health Sciences Centre
825 Sherbrook St.
Winnipeg, Manitoba R3A 1R9
Ph# 787-4615


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[Histonet] about some antiboides..

[Mehmet Fatih BOZKURT]

Hello Histonet,




I’m looking for “mmp-1, mmp-2, ER beta, TIMP-1, Collagen 1, and Collagen 3”
antibodies that work on canine tissue (dog). Does anybody have any
experiences about these antibodies? Thanks in advance for replies.


Best Wishes.

-- 
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-109
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Re: [Histonet] slide box freezer racks

[Jan Shivers]

Hello Ed,

You could check out MarketLabInc.com.   They supply "Unique & Hard-to-Find 
Products for the Clincal Laboratory".  I've purchased several things from 
them (bins, plastic storage boxes, glove holders, document displays, etc.).


Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

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- Original Message - 
From: "Ed Roy" 

To: 
Sent: Tuesday, August 23, 2011 8:34 AM
Subject: [Histonet] slide box freezer racks



Hi
I have a gazillion Fisher brand slide boxes that hold 50 slides each. We 
store the slides (fresh frozen unfixed) at -80 in freezer bags, but they 
are just piled into the freezer. Does anyone know of a source for a rack 
to organize such boxes in a -80 chest freezer?

Thanks,
Ed Roy

--

Edward Roy, PhD
Professor of Pathology
Professor of Molecular and Integrative Physiology
University of Illinois at Urbana-Champaign
506 S. Mathews Ave.
Urbana, IL 61801
217 333-3375


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[Histonet] CSF billing.

[Podawiltz, Thomas]

What is the standard CPT code that everyone uses for CSF specimens>

Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.

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[Histonet] slide box freezer racks

[Ed Roy]

Hi
I have a gazillion Fisher brand slide boxes that hold 50 slides each. We 
store the slides (fresh frozen unfixed) at -80 in freezer bags, but they 
are just piled into the freezer. Does anyone know of a source for a rack 
to organize such boxes in a -80 chest freezer?

Thanks,
Ed Roy

--

Edward Roy, PhD
Professor of Pathology
Professor of Molecular and Integrative Physiology
University of Illinois at Urbana-Champaign
506 S. Mathews Ave.
Urbana, IL 61801
217 333-3375


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