RE: [Histonet] Re: Histostar Embedding Station

[Marcum, Pamela A]

We have two.  One is about ten months old, the other a month and not a problem 
with either.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Wednesday, October 26, 2011 2:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histostar Embedding Station

Hey Jessica,

We have two Histostar's that have been here since March and have not had any
issues with them.  Can't say anything bad about them, yet.

Thanks,

Tim


Subject: [Histonet] Histostar Embedding Station
To: "Histonet@lists.utsouthwestern.edu"

Message-ID:
<1319563285.61798.yahoomail...@web121504.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Everyone,

Just curious if anyone is using the Histostar Embedding Station? And I am
curious to know how you like/dislike it? We have one and have been using it
for about 4 weeks and are already needing parts.
Thanks,

Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital


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[Histonet] Histotech Needed in Naples, FL for Day Shift-M-F (Permanent)-HOT JOB

[Melissa Phelan]

Allied Search Partners is currently looking for a qualified histology
professional for a full time/permanent position.
 
Location: Naples, FL
 
Schedule: Full Time, 6am-2:30 pm, Monday-Friday. Work one weekend per month.
Every 3rd weekend.
 
 
Requirements:
 
2 years of formal education beyond HS OR and Associates Degree
Florida License in Histology or Clinical Laboratory License
 
Summary:
 
Responsible for the preparation of tissue specimens for microscopic
examination by our Pathologist. Tissue processing, embedding, cutting,
staining, and performing other special procedures such as frozen, cytology
preparation, and bone marrow collection.
 
To apply:
 
Please send resume to meli...@alliedsearchpartners.com

 
Benefits:
 
Medical and Dental Insurance ­
Your coverage will begin the first day of the month following your 90 day
probationary period
Competitive Salaries and Shift Differentials
Free Basic Life Insurance
Accidental Death/Dismemberment Insurance
Vision Care
Flexible Spending Accounts
A generous Paid Time Off Program - up to 26 days per year
Extended Illness Bank
401 K Plan ­ 
matching contributions on the first 4% of eligible pay after the 1st year -
50% vested after 2 years
and 100% after 3 years
Holiday Pay
Staffing Incentives
Short Term Disability
Free Long Term Disability
Universal Life Insurance
Tax-sheltered Annuities
Employee Referral Bonus
2 Wellness Centers - Fitness Clubs
Lunch Payroll Deduction Program - discounts
Free Onsite Parking
Team Share/Performance Bonus
PTO Cashout
Prepaid Legal Services
College Savings Plan
At Work Weight Watchers Program
Annual Service Awards Banquet and Gifts
Monthly Service Recognition
Superstar Program - Reward and Recognition for Patient Satisfaction
Silent Heroes Recognition
Key Contributor Program ($1000 bonus)
 
 
 
 
-- 
Melissa Phelan, President Laboratory Staffing
Allied Search Partners
http://www.linkedin.com/in/melissaphelan
  P: 888-388-7571
F: 888-388-7572
C: 407-697-1175
www.alliedsearchpartners.com




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[Histonet] RE: Abs for detecting immune cells in mouse FFPE sections

[Anatoli Gleiberman]

CD3 Rabbit monoclonal from Fisher (MA1-39552) works fine in IF on mouse 
paraffin sections,
B220 rat monoclonal from eBioscience (14-0452-85) works fine in IF on mouse 
paraffin sections. Used both in 1:100 dilution.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yu, Jian
Sent: Wednesday, October 26, 2011 3:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections 

Dear all,

It will be greatly appreciated if you can share experience/information on the 
Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse 
paraffin sections.  Info on alternative Abs for these populations can be 
helpful too.  I have a small research lab with limited budget for buying new 
Abs.  

Gr-1 (granulocytes) 
B220 (B cells) 
CD3 (T cells) 

Thank you!
 
Jian Yu, Ph.D.
University of Pittsburgh

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[Histonet] Abs for detecting immune cells in mouse FFPE sections

[Jan Shivers]

CD3 - rabbit polyclonal Ab from ThermoScientific (cat. # RB-360) works on 
mouse FFPE tissue.


Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

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and then delete this message and any attachments immediately.)


- Original Message - 
From: "Yu, Jian" 

To: 
Sent: Wednesday, October 26, 2011 2:44 PM
Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections


Dear all,

It will be greatly appreciated if you can share experience/information on 
the Abs against immune cells (vendor and cat#) that work for IHC or IF 
using mouse paraffin sections.  Info on alternative Abs for these 
populations can be helpful too.  I have a small research lab with limited 
budget for buying new Abs.


Gr-1 (granulocytes)
B220 (B cells)
CD3 (T cells)

Thank you!

Jian Yu, Ph.D.
University of Pittsburgh

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[Histonet] Abs for detecting immune cells in mouse FFPE sections

[Yu, Jian]

Dear all,

It will be greatly appreciated if you can share experience/information on the 
Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse 
paraffin sections.  Info on alternative Abs for these populations can be 
helpful too.  I have a small research lab with limited budget for buying new 
Abs.  

Gr-1 (granulocytes) 
B220 (B cells) 
CD3 (T cells) 

Thank you!
 
Jian Yu, Ph.D.
University of Pittsburgh

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[Histonet] B220 and F4/80 on Ventana Discovery

[Olive Pitt]

Hello,
 
Is anyone using B220 (RA3-6B2) or F4/80 (C1:A3-1) on the Ventana Discovery?
Any protocols or tips would be greatly appreciated.  I am new to using Ventanas 
and would like to use these abs on a Discovery instead of a Dako.  Also, do all 
antibodies that work on Dako eventually work on the Ventana?  I know it is a 
closed system, so im not sure if everything eventually will work.
 
thank you for any input
-Olive
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[Histonet] Re: Histostar Embedding Station

[Tim Higgins]

Hey Jessica,

We have two Histostar's that have been here since March and have not had any
issues with them.  Can't say anything bad about them, yet.

Thanks,

Tim


Subject: [Histonet] Histostar Embedding Station
To: "Histonet@lists.utsouthwestern.edu"

Message-ID:
<1319563285.61798.yahoomail...@web121504.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Everyone,

Just curious if anyone is using the Histostar Embedding Station? And I am
curious to know how you like/dislike it? We have one and have been using it
for about 4 weeks and are already needing parts.
Thanks,

Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital


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RE: [Histonet] removal me from histo net

[Sherwood, Margaret]

To all who want to be removed from the Histonet List--you have to do it
yourself!  Go to the website and follow the directions.

 


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Raymond Stone
Sent: Wednesday, October 26, 2011 1:04 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] removal me from histo net

Please removal me for histo net
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addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


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[Histonet] removal me from histo net

[Raymond Stone]

Please removal me for histo net
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[Histonet] Antibody questions

[Sheila Fonner]

Is there anyone who is doing myc, Bap1, Pten, or CD123 for dermpath
specimens, and who is your vendor?  Do you like the products, and what
platform are you using?

 

Thanks for any information you may have.  These are new requests from our
pathologist.

 

Sheila

 

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[Histonet] EBER ISH

[Sheila Fonner]

Histonetters,

 

Is anyone out there using the EBER ISH probes from Ventana on the Ultra
Platform?  I have a few questions.  Did you need to get new software
installed?  Was there any training involved?  And do you have successful
protocols that you are willing to share?

 

I have asked the questions before, but at the time there was nobody actually
using it.  I know that there were inquiries about the protocols because of
the ASR status and the fact that Ventana cannot give  you any advice.

 

Any and all information would be greatly appreciated since we are wanting to
start it up at our facility.

 

Thanks,

Sheila

KDL Pathology

Knoxville, TN

 

 

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Re: [Histonet] saving slides between levels for IHC?

[Kim Donadio]

I think it depends on what the specimen is. All of the places Ive worked have 
always kept extras(levels) on sentinal nodes for keratins, also for prostate 
needle biopsies for p63/504s or other IHC's. 
 
Really doesnt it come down to what the Pathologist needs to make a diagnosis 
for the patient? 
 
I know it's a pain to cut so many levels and save them. Its also costly. But 
trust me, none of that is as bad as the sinking feeling you get when a biopsy 
has been exhausted and they need more stains :(
 
Hope this helps. Just my 2 cents worth...
 
Kim Donadio



From: Sheila Fonner 
To: "'Harrison, Sandra C.'" ; 
histonet@lists.utsouthwestern.edu
Sent: Wednesday, October 26, 2011 7:28 AM
Subject: RE: [Histonet] saving slides between levels for IHC?

Sandy,

I don't think it is a "standard" procedure.  However, our pathologist will
frequently ask us to keep intervening levels for possible IHC on certain
cases that are small or where the tumor is about to be exhausted.  We are a
dermatopathology lab.  When it is requested, we cut the whole case on a
clean waterbath, using distilled water and no additives, on plus slides.
The routine H&E's are stained normally and the other slides are dried and
held for possible immunos.

Sheila
KDL Pathology
Knoxville, TN


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison,
Sandra C.
Sent: Tuesday, October 25, 2011 2:00 PM
To: Webb, Dorothy L; Horge, Pamela J; rollmann.de...@mayo.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] saving slides between levels for IHC?

Does anyone know if it is standard  to save sections from between the
levels for IP?  Is it standard at your institution?





Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449



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[Histonet] RE: Please remove my name

[Bea DeBrosse-Serra]

If you want to be removed from the list, you have to do it yourself by going to 
the website below.

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Brown
Sent: Wednesday, October 26, 2011 6:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Please remove my name

Hello, 

Please remove my name from the Histonet mailing list.

Thank you,

Roger Brown (rbr...@mst.edu)


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[Histonet] Please remove my name

[Vanessa J. Phelan]

Hi, please remove my name from the mailing list.

Thank you

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[Histonet] Please remove my name

[Roger Brown]

Hello, 

Please remove my name from the Histonet mailing list.

Thank you,

Roger Brown (rbr...@mst.edu)


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[Histonet] RE: Re: Seeking Beta-Amyloid not needing FA pretreatment

[Connolly, Brett M]

Interestingwe see APP on frozen sections with 6E10, but not in paraffin 
sections after citrate HIER. We use the monoclonal from Covance, 1:12000 
overnight at 4C. 

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Wednesday, October 26, 2011 2:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment


Be careful: HIER may expose not only extracellular amyloid plaques but also 
intracellular APP ( or...is it intracellular A beta  ;-))
Eg:  with 6E10 it is directed against an epitope found in APP and also A 
beta: if you do Formic acid, only extracellular plaques are positive...after 
HIER, there is also intracellular positivity.
In my experience.
Some examples of this can be found here, in the image gallery : 
http://www.immunoportal.com/


Whichever way you gogood luck.

Carl


Carl Hobbs
Histology Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813

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RE: [Histonet] saving slides between levels for IHC?

[Sheila Fonner]

Sandy,

I don't think it is a "standard" procedure.  However, our pathologist will
frequently ask us to keep intervening levels for possible IHC on certain
cases that are small or where the tumor is about to be exhausted.  We are a
dermatopathology lab.  When it is requested, we cut the whole case on a
clean waterbath, using distilled water and no additives, on plus slides.
The routine H&E's are stained normally and the other slides are dried and
held for possible immunos.

Sheila
KDL Pathology
Knoxville, TN


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison,
Sandra C.
Sent: Tuesday, October 25, 2011 2:00 PM
To: Webb, Dorothy L; Horge, Pamela J; rollmann.de...@mayo.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] saving slides between levels for IHC?

Does anyone know if it is standard  to save sections from between the
levels for IP?  Is it standard at your institution?

 

 

Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449

 

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