[Histonet] Dried Tissue Marking Ink
I've been having issues with some of my tissue marking ink drying up. Is there any solvent I can add to reconstitute it, or do I just need to get new bottles? -- J Emahiser Mohs Tech ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EM question
I am the first to admit I know little to nothing about EM. I have a researcher who read an article today that said in the method that they had taken cells, applied them to copper EM grids and immersed them in liquid nitrogen to fix them. Has anyone ever heard of this technique? and if you have, do you have any directions or references for this technique? Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ? QIHC
Contemplating the IHC qualification and wondering if supervisors/managers can provide feedback as to their feeling/perception of this credential, how you feel it might effect/limit hiring for you when you review candidates? Want to make sure that I am investing in something that will truly aid my marketability. Thanks Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] counterstain for fast red
Try 1-2% Ethyl green in acetate buffer (pH4) - will give you green nuclei (of course) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, 15 November 2011 3:59 AM To: Histonet Subject: [Histonet] counterstain for fast red Hi Everyone, We are currently running some alk phos staining protocols with a fast red chromogen. My pathologist would like to use something other than Hematoxylin as a counterstain. What does everyone recommend? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: 15 years of Histonet
Can't agree more. I mean, where would Joe the Toe be without the Histonet? By the way, just read the reviews from the lecture my buddy Hector and I gave in Cinci this year. One of the critiques stated that Hector and I are the biggest egomaniacs this side of space. Wow, I've been called a lot of things in my life, but an egomaniac? Really? That hurt, right to the core. JTT - Original Message - From: "Morken, Timothy" To: Sent: Monday, November 14, 2011 10:32 AM Subject: [Histonet] RE: 15 years of Histonet Linda Wrote "By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally)." That is really great. I agree with John Kiernan that Histonet really opened up the histology community. Back in the "old" days you were lucky if you knew a few techs outside your own lab, and met a few at the occasional state or national meeting. Most histotechs were very isolated. Since Histonet that has all changed. Now we are all just an email away from getting just about any question answered. I am continually astonished at the breadth of knowledge out there AND the breadth of the field. I think I heard about Histonet from NSH. In any case it was around 1997 and I was working in Saudi Arabia. Histonet was a fantastic resource and I even ended up getting a job back in the US through contacts I made on Histonet (remember that Jeannine?). Since then I peruse it almost daily and occasionally post. In any case it is always interesting to see what the unofficial "topic of the day" is! Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, November 11, 2011 12:01 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribing from Histonet Dear Histonetters: Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: http://lists.utsouthwestern.edu/mailman/options/histonet You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know.If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. Thanks, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwes
[Histonet] Second Shift lead position available at CellNetix Labs in Seattle
Histonet, *Job Title:*Lead Histotech – 2nd shift *Department: *Laboratory *Reports To:*Histology Supervisor *Shifts: *3:00pm to 11:30PM Monday thru Friday *SUMMARY* Under the supervision of the Histology Supervisor, the Lead Histology Technician/Technologist, performs with proficiency and understanding, the tests and procedures assigned to the Histology Department. The position is responsible for grossing, processing, embedding, cutting and staining of tissue blocks, accurate reporting of results, quality control, teaching, special projects, equipment maintenance and all other duties assigned by the Supervisor. * * *ESSENTIAL FUNCTIONS* ·Performs both routine and complex special procedures with the understanding of tissue structures, techniques, principles, theory and instrumentation. ·Able to gross section and dictate descriptions of simple specimens, process, embed, cut and stain all types of tissue specimens received in the Histology Department. ·Coverslip stained sections, verify that the staining shows adequate cellular distinction, label, and distribute to the pathologist responsible for reviewing the case. ·Assures specimen labeling requirements are met and necessary clinical information is available as needed. ·Assist pathologists with frozen section staining and processing. ·Assists laboratory assistant or performs accessioning of patient specimens into laboratory computer system when needed. ·Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. ·Selects and maintains appropriate tissue blocks to serve as controls for Special Satins. Maintains all documentation that insures that all Special Stain control blocks have been adequately tested. Cuts and labels control slides for Histology, Special Stains and IHC. ·Able to process and decal bone specimens. ·Recognize and troubleshoot both routine and complex problems and assist other Histotechs and lab assistants with technical problems. ·Monitor quality control results and take immediate and proper action when controls are unacceptable. Follow defined procedures with only supervisor or pathologist approved modifications or deviations. ·Complete all instrument function verification, maintenance, and documents according to procedure in Histology area. Ensure that equipment defects and malfunctions are reported and repaired. ·Maintains a neat, clean, and orderly work area. At the end of the day’s work; is responsible for the cleaning of his/her microtome, filing his/ her cut blocks and cleaning any soiled glassware. ·Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. ·Cuts slides for IHC testing or other sendouts in conformance with procedures and submits them for analysis. ·Takes on additional responsibility for training Laboratory personnel including Laboratory Assistants, NRT’s and other Histotechs in Histology procedures. ·Maybe be asked to write/ update Operating Procedures. ·Must be able to express himself/ herself effectively both in written and verbal communications. ·Assists supervisory staff in monitoring workflow and insuring that work/ staining priorities are met for the department. ·Will be asked to put new tests/ stains on line. ·May assist pathologists or physicians in the collection of specimens from patients. ·Participates in special projects or other duties as assigned by supervisor staff or company. ·Always maintains a pleasant, courteous attitude when answering the telephone. ·Conforms to laboratory timekeeping procedures. Keeps unexcused absences and late arrivals to a minimum. ·Participates in weekend and holiday schedules as staffing requirements dictate. Remains flexible and works a share of overtime or different shifts if necessary during staffing shortages or emergencies. ·Participates in continuing education classes and courses. Strongly encouraged to keep updated on recent advances in the field of histology and to take at least 10 credit hours of continuing education a year. ·Highly encouraged to maintain membership in a professional Histology organization. ·Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. ·Conforms to established and new procedures and policies instituted by the company. ·Conforms to the Laboratory Dress code and wears appropriate name badge at all times. ·Able to accept guidance and constructive criticism. Displays a mature and profession
[Histonet] rat whiskers
&nb= sp;Instead of treating the whiskers, perhaps embedding the follicles on aga= r and then processing the agar, cutting the agar/paraffin block with a new = balde, keeping every section, you should be okay. You can also try and cut = them at 3 microns. I would practice this several times first. &nb= sp; Nick(Rocky) Madary, HT/HTL= (ASCP)QIHC On 11/14/11, histonet-request@lists.utsouthw= estern.edu wrote: Send Histonet mailing list submissions to [1]histonet@lis= ts.utsouthwestern.edu To subscribe or unsubscribe via the World = Wide Web, visit [2]http://lists.utsouthwestern.= edu/mailman/listinfo/histonet or, via email, send a message with sub= ject or body 'help' to [3]histonet-request@lists.utsouthwestern.= edu You can reach the person managing the list at [4]his= tonet-ow...@lists.utsouthwestern.edu When replying, please edit = your Subject line so it is more specific than "Re: Contents of Histonet = digest..." Today's Topics: 1. RE: HTL Exam (joelle weaver= ) 2. Whiskers (Adam Boanas) 3. using Aquoes mounting media (Remedy Bi= ) 4. RE: HTL Exam (joelle weaver) 5. (no subject) (Freeman, Carol) 6. RE: 15 years of Histonet (Morken, Timothy) 7. counterstain for fast = red (Kim Merriam) 8. HT training -competency checklist (joelle weaver) - - Message: 1 Date: Mon, 14 Nov 2011 14:11:19 + From: j= oelle weaver <[5]joelle= wea...@hotmail.com> Subject: [Histonet] RE: HTL Exam To: <<= A class=parsedEmail href="mailto:aaperg...@uspath.com"; target=_blank _djrealurl="mailto:aaperg...@uspath.com";>aaperg...@uspath.com>, Hi stonet <[6]histonet@lists.utsouthwestern.edu>, <[7]histo= net-boun...@lists.utsouthwestern.edu> Message-ID: Content-Type: text/plain; charset="iso-8859-= 1" AdrienneThanks for your thoughts, however I was not in need o= f any study help, having passed the exam some time ago, but was posting in response to students questions to try to help and support them. I think you= r system is good if your goal is memorization. My personal feeling is that = while this may allow you to select the correct answer on any MC test in the= short term; if you only memorize information it will leave short term reca= ll very quickly, and you will be lacking when called upon to use this infor= mation for application, synthesis, troubleshooting etc., in a real lab situ ation. Memorization is a learning first step, but good to move beyond this = level of understanding in your learning process. I am glad that you were ab= le to pass, and I hope you go on to build on your learning through your wor= k in the lab. Best of luck. Joelle Weaver MAOM, BA, (HTL) ASCP [9]http:/= /www.linkedin.com/in/joelleweaver > Date: Fri, 11 Nov 2011 12= :38:24 -0700 > From: [10]aape= rg...@uspath.com > To: [11]joellewea...@hotmail.com > Subject: HTL Exam > > Hi Joelle, > > > I took the HTL exam in April. I= thought I did terribly but ended up passing (go figure). > >= I honestly think it's a lot of memorization. > > I got the f= lash-cards that compliment the Carson text. Some images were repeats of the= book but most were new. And it was an excellent study guide for questions = like: what is this stain, what's wrong with this stain/section, what's the = best fixative for this stain, etc. I also made my own flashcards, of which = I had probably around 500. Not an easy task. > > I read and h= igh-lighted the Carson and Barncroft text. Then I went through and made an = outline of the text (a lng outline). > After reviewing that a fe= w times, I made my flashcards. > > Going through the flashcar= ds over the weeks, I could eventually cut out the ones that I knew the answ= ers to and kept studying the more difficult ones. > > The Bar= ncroft text was an excellent supplemental for Carson and also had nice imag= es. > > If you would like my outline, let me know and I will s= end it over. $500. Kidding. > > Best of luck! > &g= t; Adrienne > > -- > > > > Adrienn= e Kavanagh HTL (ASCP) > US PATH > 30 W. Century Road >= Suite 255 > Paramus NJ 07652 --= Message: 2 Date: Mon, 14 Nov 2011 15:13:05 + From: Ad= am Boanas <[12]a.boanas@epis= tem.co.uk> Subject: [Histonet] Whiskers To: "[13]histonet@lists.utsouth= western.edu" <[14]histonet@lists.utsouthwestern.edu> Message-ID: <176C97AAA877D24798ED73[15]76652D0FD815AEE4F263@SRVEXCH 02.epistem.local> Content-Type: text/plain; charset="us-ascii"<= BR> Hello, I am currently sectioning individual rat whiskers and = follicles and am having some trouble obtaining cl
[Histonet] HTL exam
&nb= sp;Although I paased it when there was still a practical, I found that read= ing ALL of the recommended texts provided me with the information needed to= pass the exam without the need to memorize. I esp found the B&S and th= e Carson text particularly helpful and it was great to read them again.If y= ou work in a histolab and pay attention to what you are doing, you will lik= ely pas the exam or at least get half the answers from just being engaged o= n the job. Memorization will not help with questions that require you to re cognize what a stain is, an artifact, troubleshooting etc. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC On 11/14/11, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to [1]histonet@lists.utsouthwest= ern.edu To subscribe or unsubscribe via the World Wide Web, visi= t [2]http://lists.utsouthwestern.edu/mailman/li= stinfo/histonet or, via email, send a message with subject or body '= help' to [3]histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lis= ts.utsouthwestern.edu When replying, please edit your Subject li= ne so it is more specific than "Re: Contents of Histonet digest..." <= BR> Today's Topics: 1. RE: HTL Exam (joelle weaver) 2. Whisker= s (Adam Boanas) 3. using Aquoes mounting media (Remedy Bi) 4. RE: HTL= Exam (joelle weaver) 5. (no subject) (Freeman, Carol) 6. RE: 15 year= s of Histonet (Morken, Timothy) 7. counterstain for fast red (Kim Merria= m) 8. HT training -competency checklist (joelle weaver) - - Me= ssage: 1 Date: Mon, 14 Nov 2011 14:11:19 + From: joelle weaver &l= t;[4]joelleweaver@hotmail.= com> Subject: [Histonet] RE: HTL Exam To: <[5]aaperg...@uspath.com>, Histonet <<= A class=parsedEmail href="mailto:histonet@lists.utsouthwestern.edu"; tar= get=_blank _djrealurl="mailto:histonet@lists.utsouthwestern.edu";>histon e...@lists.utsouthwestern.edu>, <[6]histonet-bounces@lis= ts.utsouthwestern.edu> Message-ID: Content-Type: text/plain; charset="iso-8859-1" A= drienneThanks for your thoughts, however I was not in need of any study hel= p, having passed the exam some time ago, but was posting in response to stu= dents questions to try to help and support them. I think your system is goo= d if your goal is memorization. My personal feeling is that while this may = allow you to select the correct answer on any MC test in the short term; if= you only memorize information it will leave short term recall very quickly= , and you will be lacking when called upon to use this information for appl ication, synthesis, troubleshooting etc., in a real lab situation. Memoriza= tion is a learning first step, but good to move beyond this level of unders= tanding in your learning process. I am glad that you were able to pass, and= I hope you go on to build on your learning through your work in the lab. B= est of luck. Joelle Weaver MAOM, BA, (HTL) ASCP [8]http://www.linkedin.c= om/in/joelleweaver > Date: Fri, 11 Nov 2011 12:38:24 -0700 > From: [9]aaperg...@uspath.co= m > To: [10]joell= ewea...@hotmail.com > Subject: HTL Exam > > Hi Joell= e, > > > I took the HTL exam in April. I thought I did = terribly but ended up passing (go figure). > > I honestly thi= nk it's a lot of memorization. > > I got the flash-cards that= compliment the Carson text. Some images were repeats of the book but most = were new. And it was an excellent study guide for questions like: what is t= his stain, what's wrong with this stain/section, what's the best fixative f= or this stain, etc. I also made my own flashcards, of which I had probably = around 500. Not an easy task. > > I read and high-lighted the= Carson and Barncroft text. Then I went through and made an outline of the = text (a lng outline). > After reviewing that a few times, I made= my flashcards. > > Going through the flashcards over the wee= ks, I could eventually cut out the ones that I knew the answers to and kept= studying the more difficult ones. > > The Barncroft text was= an excellent supplemental for Carson and also had nice images. > > If you would like my outline, let me know and I will send it over. $5= 00. Kidding. > > Best of luck! > > Adrienne = > > -- > > > > Adrienne Kavanagh HTL = (ASCP) > US PATH > 30 W. Century Road > Suite 255 = > Paramus NJ 07652 -- Mes= sage: 2 Date: Mon, 14 Nov 2011 15:13:05 + From: Adam Boanas <<= A class=parsedEmail href="mailto:a.boa...@epistem.co.uk";target=_blan=k
[Histonet] RE: Histonet Digest, Vol 96, Issue 20
Carol- We started doing the MSI panel on all colon resections earlier this year. We run this on the BOND system with good results. Nancy Dubuque, IA Message: 5 Date: Mon, 14 Nov 2011 11:15:19 -0500 From: "Freeman, Carol" Subject: [Histonet] (no subject) To: Message-ID: Content-Type: text/plain; charset="us-ascii" We are starting MSI testing in our lab, I am just curious if there is any labs out there doing this on colon biopsy cases. If so we would love some feedback. I would like to know if this is common practice somewhere. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT training -competency checklist
JenniferI got a linked in account and have never paid for it since 2008.I think that you pay only if you want the enhanced page features. I think that the checklist builder is under the lab quality management and competency materials from CAP. I posted the link the other day by entering competency in the search bar. I got to it even though I have not renewed my CAP membership as of yet. My own documents I posted as a word doc from slide share. On my profile, I believe that you can click on the presentations link and then it will show what I have posted at the moment. If none of this works, let me know and if you give me more specifics, perhaps I can help you with your project in a more individualized way? I don't think that the document I posted would be at enough detail to be used by any specific lab anyhow as it is, just trying to help out if someone is struggling to get started since I have worked with this exact project for awhile. Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > From: jwri...@molecularmd.com > To: joellewea...@hotmail.com > Date: Mon, 14 Nov 2011 10:51:11 -0800 > Subject: RE: [Histonet] HT training -competency checklist > > Hi Joelle, > > I tried to click on the CAP website link and I got... Tips & Tricks - Fall > 2008 > Share Your Own Tips and Tricks > Share Your Own Tips and Tricks. > > I also tried to access your linked in page, I even signed up for a linked in > account, when I tried to access your page I was prompted to pay a yearly > membership fee. > > Please help, I really don't want to join linked in but I would like to see > what you use as training checklist. I promise I will not copy and paste yours > and try to pass it off as my own :-) > > Thank-you, > > Jennifer Wright, HTL (ASCP)cm, QIHC > Senior Histology Technologist > MolecularMD Corp. > 1341 SW Custer Drive > Portland, OR 97219 > Office: 1-503-459-4974 Ext. 158 > Fax: 1-503-459-4976 > jwri...@molecularmd.com > www.molecularmd.com > > > This document and any associated attachments may contain information that is > privileged, CONFIDENTIAL, and exempt from disclosure under applicable law. > If > you are not the intended recipient, please notify me immediately, as the use > of > this information is strictly prohibited. > > > > > > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: 14 November, 2011 9:47 AM > To: histonet-boun...@lists.utsouthwestern.edu; Histonet > Subject: [Histonet] HT training -competency checklist > > > A couple of people contacted me about a training and competency checklist for > intial, entry level HT employees. I put something together that is pretty > generic from some items I developed for a previous employer. Of course if I > was going to do this for myself, I would expand it out, but it might help > those out who are working to develop this instrument for their lab. I will > post from my linked in page and if it helps out, great. Just keep in mind > that this would not be a copy/paste item and that you might use this as an > idea starter only, and would need to add your specific instrumentation > processes and procedures, LIS and other SOP's to be incorporated. I still > recommend the CAP tool, and also the need to correlate with the job > description, and broader organizational objectives, which of course would > vary by laboratory.ThanksJoelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pan Keratin Mouse
Hi All Anyone have any recommendations for a pan cytokeratin that works in mouse tissue? Thanks Luis This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] using Aquoes mounting media
For sure the one you bought come with the instructions. In essence your section has to be wet (with water); add the mounting medium and cover. Let it dar. René J. --- On Mon, 11/14/11, Remedy Bi wrote: From: Remedy Bi Subject: [Histonet] using Aquoes mounting media To: histonet@lists.utsouthwestern.edu Date: Monday, November 14, 2011, 10:29 AM Hello friends i wish to fine out how to use the aqueous mounting media. thanks alot ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whiskers
Us 10% aq. KOH sol. for half an ahour and process after that. René J. --- On Mon, 11/14/11, Adam Boanas wrote: From: Adam Boanas Subject: [Histonet] Whiskers To: "histonet@lists.utsouthwestern.edu" Date: Monday, November 14, 2011, 10:13 AM Hello, I am currently sectioning individual rat whiskers and follicles and am having some trouble obtaining clean sections from within the centre of the whisker (and intact bulb) without the brittle hair falling out of the block or splintering. Does anyone know of a method that I could use to soften the keratin prior to embedding? I am thinking 10% Sodium Hydroxide or 5% Ammonium Hydroxide but do not really know about appropriate timings for such a small tissue. Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT training -competency checklist
A couple of people contacted me about a training and competency checklist for intial, entry level HT employees. I put something together that is pretty generic from some items I developed for a previous employer. Of course if I was going to do this for myself, I would expand it out, but it might help those out who are working to develop this instrument for their lab. I will post from my linked in page and if it helps out, great. Just keep in mind that this would not be a copy/paste item and that you might use this as an idea starter only, and would need to add your specific instrumentation processes and procedures, LIS and other SOP's to be incorporated. I still recommend the CAP tool, and also the need to correlate with the job description, and broader organizational objectives, which of course would vary by laboratory.ThanksJoelle Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] counterstain for fast red
Hi Everyone, We are currently running some alk phos staining protocols with a fast red chromogen. My pathologist would like to use something other than Hematoxylin as a counterstain. What does everyone recommend? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: 15 years of Histonet
Linda Wrote "By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally)." That is really great. I agree with John Kiernan that Histonet really opened up the histology community. Back in the "old" days you were lucky if you knew a few techs outside your own lab, and met a few at the occasional state or national meeting. Most histotechs were very isolated. Since Histonet that has all changed. Now we are all just an email away from getting just about any question answered. I am continually astonished at the breadth of knowledge out there AND the breadth of the field. I think I heard about Histonet from NSH. In any case it was around 1997 and I was working in Saudi Arabia. Histonet was a fantastic resource and I even ended up getting a job back in the US through contacts I made on Histonet (remember that Jeannine?). Since then I peruse it almost daily and occasionally post. In any case it is always interesting to see what the unofficial "topic of the day" is! Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, November 11, 2011 12:01 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribing from Histonet Dear Histonetters: Hi, I haven't put out many messages on the list recently. I have just been quietly administering the list (and occasionally removing people who ask to be unsubscribed as my time allows). By the way, Histonet is coming up on its 16th year anniversary (wow!) and there are currently 3543 members on the list (from >30 countries as of my last tally). As it keeps coming up, if you wish to unsubscribe or modify your account options, go to this link: http://lists.utsouthwestern.edu/mailman/options/histonet You will need to request your password if you don't know or remember it. I tried to get this link added to the bottom of all the messages but apparently that is not possible with the current software. If you have trouble getting off the list, let me know.If your email address has been changed at your organization, you may have trouble posting messages or unsubscribing. If so, let me know (but provide me with the old address that is on the membership list) and I'll try to help. Thanks, Linda M Histonet administrator Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
We are starting MSI testing in our lab, I am just curious if there is any labs out there doing this on colon biopsy cases. If so we would love some feedback. I would like to know if this is common practice somewhere. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HTL Exam
No harm done. I believe that you meant the Bancroft text, and it is also good and similar in content to the Sheehan & Hrapchak as well as the Pierce and Luna publication(s) . All would suffice in my opinion, as a compliment to Carson, it is more or less a matter of opinion and preference, though you have to keep in mind the publication timelines of those. Your flashcards would be helpful to students I believe, though in my opinion the process of creating the flashcards or outlines yourself is probably as valuable to the intial memorization, as using them as memorization tools.There are considerably more good resources available now than in the past, and whatever methods work best for your learning- go for it. Given my own past experiences, I merely emphasize the practical application of the knowledge in the lab as the final, yet most important, step. However, I recognize and encourage ANYONE who attempts the HTL, however they choose to pursue it, we really need more educated, knowledgable people in the hiring pool! Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Mon, 14 Nov 2011 07:08:41 -0700 > From: aaperg...@uspath.com > To: joellewea...@hotmail.com > Subject: Re: HTL Exam > > I just realized I sent this to you instead of the one asking about the exam. > > My apologies! > > > > - Original Message - > From: "Adrienne Aperghis Kavanagh" > To: joellewea...@hotmail.com > Sent: Friday, November 11, 2011 2:38:24 PM > Subject: HTL Exam > > Hi Joelle, > > > I took the HTL exam in April. I thought I did terribly but ended up > passing (go figure). > > I honestly think it's a lot of memorization. > > I got the flash-cards that compliment the Carson text. Some images were > repeats of the book but most were new. And it was an excellent study > guide for questions like: what is this stain, what's wrong with this > stain/section, what's the best fixative for this stain, etc. I also made > my own flashcards, of which I had probably around 500. Not an easy task. > > I read and high-lighted the Carson and Barncroft text. Then I went > through and made an outline of the text (a lng outline). > After reviewing that a few times, I made my flashcards. > > Going through the flashcards over the weeks, I could eventually cut out > the ones that I knew the answers to and kept studying the more difficult > ones. > > The Barncroft text was an excellent supplemental for Carson and also had > nice images. > > If you would like my outline, let me know and I will send it over. $500. > Kidding. > > Best of luck! > > Adrienne > > -- > > > > Adrienne Kavanagh HTL (ASCP) > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 > > -- > > > > Adrienne Kavanagh HTL (ASCP) > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] using Aquoes mounting media
Hello friends i wish to fine out how to use the aqueous mounting media. thanks alot ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whiskers
Hello, I am currently sectioning individual rat whiskers and follicles and am having some trouble obtaining clean sections from within the centre of the whisker (and intact bulb) without the brittle hair falling out of the block or splintering. Does anyone know of a method that I could use to soften the keratin prior to embedding? I am thinking 10% Sodium Hydroxide or 5% Ammonium Hydroxide but do not really know about appropriate timings for such a small tissue. Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HTL Exam
AdrienneThanks for your thoughts, however I was not in need of any study help, having passed the exam some time ago, but was posting in response to students questions to try to help and support them. I think your system is good if your goal is memorization. My personal feeling is that while this may allow you to select the correct answer on any MC test in the short term; if you only memorize information it will leave short term recall very quickly, and you will be lacking when called upon to use this information for application, synthesis, troubleshooting etc., in a real lab situation. Memorization is a learning first step, but good to move beyond this level of understanding in your learning process. I am glad that you were able to pass, and I hope you go on to build on your learning through your work in the lab. Best of luck. Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver > Date: Fri, 11 Nov 2011 12:38:24 -0700 > From: aaperg...@uspath.com > To: joellewea...@hotmail.com > Subject: HTL Exam > > Hi Joelle, > > > I took the HTL exam in April. I thought I did terribly but ended up passing > (go figure). > > I honestly think it's a lot of memorization. > > I got the flash-cards that compliment the Carson text. Some images were > repeats of the book but most were new. And it was an excellent study guide > for questions like: what is this stain, what's wrong with this > stain/section, what's the best fixative for this stain, etc. I also made my > own flashcards, of which I had probably around 500. Not an easy task. > > I read and high-lighted the Carson and Barncroft text. Then I went through > and made an outline of the text (a lng outline). > After reviewing that a few times, I made my flashcards. > > Going through the flashcards over the weeks, I could eventually cut out the > ones that I knew the answers to and kept studying the more difficult ones. > > The Barncroft text was an excellent supplemental for Carson and also had nice > images. > > If you would like my outline, let me know and I will send it over. $500. > Kidding. > > Best of luck! > > Adrienne > > -- > > > > Adrienne Kavanagh HTL (ASCP) > US PATH > 30 W. Century Road > Suite 255 > Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
