[Histonet] gelatine, silane, super frost plus slides ???

[Daniela Bodemer]

Hi all,

 

I am putting information together for students and thought you might be
able to help me.

Gelatine, silane, super frost plus slides. What are the differences and
characteristics and which slide to use for what?

 

Thank you,

 

Daniela Bodemer 


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[Histonet] Seeking a Histology Supervisor in Connecticut

[Darcy Bloch]

   Slone=  Partners  seeks  a Histology Supervisor for our hospital based
   laboratory= client in Connecticut.
   

   
The  successful  candidate  will  have=  experience  with  workflow
   redesign  and  be  great  at  managing change. There= are currently 38
   people in the department, including 2 supervisors. Lean= and Six Sigma
   experience is a plus.
   

   
Qualified  candidates=  will  have  a B.S. degree and either HT/HTL
   certification,  with  supervisory=  experience,  preferably in a busy,
   high-volume laboratory.
   

   = 
Special features of this position: The histology supervisor= will
   have the opportunity to help redesign this busy laboratory.
   = 

   
If=  you  meet these qualifications and would like to be considered
   for  this=  position,  please  submit  your  resume  to Darcy Bloch at
   [1]dar...@slonepartners.com.
   

   
If  you  have experience in the diagnostic laboratory industry= and
   wish  to be considered for other roles, please forward your resume to   Tara 
Kochis at [2]t...@slonepartners.com.
   

   
All= inquiries are kept confidential.

References

   1. 3D"mailto:dar...@slonepartners.com";
   2. 3D"mailto:t...@slonepartners.com";
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[Histonet] Histotech needed in Texas

[Tunde Ajibade]

I have a full-time and permanent position for histotech in my department; this 
position opens due to retiring histotech.Interested applicants can contact me 
at the number below for more details or e-mail your resume.

 Tunde Ajibade BS, HTL(ASCP)QIHC
 Histology Supervisor
 Medical Center Hospital
 Odessa,TX
 Tel:432-640-2348
 Fax:432-640-2303



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[Histonet] Anyone tried the Primera Signature Slide Printer?

[Li, Nathan]

Hello Histonetters,
I was wondering if anyone out there has tried the Primera Signature Slide 
Printer.  It boasts color printing and is geared towards smaller labs (like 
mine I suppose).  I'm fairly sure it's a new printer on the market and would be 
happy if anyone out there in histoland could share their thoughts on it.  Ink 
cartridge changes, maintenance or type of slide used.

Thanks,
Nathan
Tufts University
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[Histonet] Morgue Covers

[Dana Spencer]

I was wondering what policies/ procedures others are using for cleaning/ 
disinfecting Morgue Covers.  How frequently do you clean and what kind of 
covers are you using?
 
Thanks,
Dana 

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[Histonet] Histology Supervisor Job Opening in Naples, FL

[Melissa Phelan]

Allied Search Partners is conducting a search for a qualified Histology
Supervisor. This candidate must be Florida Licensed as a Supervisor and have
a Bachelor¹s Degree or higher to qualify for this position. Please let me
know if you, or anyone that you know would be a great fit for this job.
 
Position:  Histology Supervisor
Shift:  8am-4:30pm Full time/Permanent
Location:  Naples, FL
 
To Apply:  Please send current resume as well as salary expectations to
meli...@alliedsearchpartners.com for review.  Qualified applicants will be
contacted by one of our recruiters for a phone prescreen.
 
Position Summary:  
Allied Search Partners is looking for a qualified Histology Supervisor for a
state-of-the-art laboratory in Naples, FL.
 
· Provides direct supervision to the Histology section coordinating
workflow and overseeing the scheduling of staff.

· Laboratory Supervisor maintains instruments, monitors proficiency
testing and quality control, actively participates in performance
improvement, and develops new procedures.

· Facilitates employee training and competence assessment.

· Maintains supplies and inventory in a cost effective manner.

· Develops laboratory personnel through continuing education
opportunities and delegates special projects where appropriate.

· Complies with and enforces policies and procedures in a consistent and
timely manner with a fair consideration for the associate.

· Assures minimal downtime of instruments by planning for preventative
maintenance and contacting service personnel when unavoidable repairs are
necessary.

· Assesses costs of supplies and reviews inventory levels and usage
rates to ensure adequate supplies are stocked to meet workload demands.

 
Requirements:
· BA/BS in related field required

· Current Florida licensure as a Histology Supervisor

· 3+ years clinical laboratory experience

-- 
Melissa Phelan, President Laboratory Staffing
Allied Search Partners
http://www.linkedin.com/in/melissaphelan
  P: 888-388-7571
F: 888-388-7572
C: 407-697-1175
www.alliedsearchpartners.com




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[Histonet] PAX 2 antibody

[Martha Ward-Pathology]

Hello all,

I am still hunting for a source for the PAX 2 antibody.Is this antibody 
currently available?   I have heard in the past that there was a manufacturing 
issue.

Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

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[Histonet] Tissue Processor

[Patricia Bourassa]

Hello!

We are looking to purchase a used tissue processor for a small biotech.  We
will be working with calcified bone so we will only use the machine for
dehydration and will infiltrate with methacrylate by hand.

I'm interested in suggestions.  It's been a while since I've done histology
and I'd like to know what machines/ vendors folks would recommend.

Thanks for your help!  Patti

-- 
*Patti Bourassa
Senior Scientist
Karos Pharmaceuticals
(203) 535-0540, ext 207*
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[Histonet] Gayle Callis

[Patricia Bourassa]

I'm trying to contact Gayle... is she still on the histonet?
-- 
*Patti Bourassa
Senior Scientist
Karos Pharmaceuticals
(203) 535-0540, ext 207*
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Re: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain

[Kim Merriam]

I was having similar issues with frozen rat and human cartilage with the 
cryojane.  After a lot of trial and error; I have found that the 4X slides and 
air-drying overnight has worked extremely well for us.  The tissues never fall 
off any more!
 
Good luck,
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA



From: "Connolly, Brett M" 
To: Douglas M Burns ; "histonet@lists.utsouthwestern.edu" 
 
Sent: Thursday, December 8, 2011 9:46 PM
Subject: RE: [Histonet] brain sections cut on Cryo Jane apparatus are falling 
off slides during ethanol series for a cresyl stain

Doug,
Hopefully you are using the CryoJane slides (not the subbed slides) and the UV 
glue activator and keeping both slides and tape cold in the cryostat. Be aware 
that you can get CryoJane slides with different amounts of adhesive (0.5-4x).  
I would suggest going to the 4x slides. When peeling the tape off I pull it 
slowly and evenly almost back on itself at an acute angle- this greatly reduces 
the incidence of the section coming off with the tape... Haven't had a problem 
with section detachment in ethanols.

Brett

Brett Connolly, Phd
Merck Research Labs
West Point, PA


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns 
[dmbur...@gmail.com]
Sent: Thursday, December 08, 2011 5:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] brain sections cut on Cryo Jane apparatus are falling off 
slides during ethanol series for a cresyl stain

Hello, Histo-users,

    Follow-up to our original posting about fixed-brain cryosections
falling off of subbed slides. We fished out our Cryo-Jane apparatus, and
using this apparatus has resulted in a great improvement in sections and
getting sections stuck onto the slides. This works pretty well until we
start using the ethanol series to dehydrate and delipidate before a Crystal
violet stain for Nissl bodies. (That is, for acqueous steps everything
seems to stay glued to the slide; however, once ethanols are involved
pieces start to come off.)

      To summarize, we are trying to go from cut blocks of
paraformaldehyde-fixed brain infused with sucrose and then frozen at -80.
We take them out, unfrozen, and cut them on a Leica cryostat. Early
attempts with subbed slides did not work reliably, so we have turned to
'Jane. Jane is slightly slower than a brush, and seems to work fairly well.
However, we have had trouble with the tape pulling parts of sections off of
the glued slides. When we introduce the nonacqeous solvents, our problems
become more obvious.

      We  have not so far gone to a post-fix, because we wanted to
simplify the overall process. Could  this be the mistake?

      We want to use IHC for c-Fox and possibly orexin - and similar - but
we want cresyl-stain to locate positively the area of interest in the cut
block.

      Any suggestions?  Also, are there any 'Jane enthusiasts out there?

                            thank  you in advance  ---  Doug
Burns, Kansas City
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