RE: [Histonet] anti static spray?

2012-02-10 Thread Ingles Claire
 
Are you sure it isn't just your electric personality? :)
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio
Sent: Fri 2/10/2012 2:15 PM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] anti static spray?



I have this problem a lot. I have static so bad literally blue sparks fly from 
my finger tips. I'm scared I'm going to blow up something one day and I walk 
around terrified to open doors. Anyway, in order to remedy my problem during 
sectioning I use a lot of hand lotion and get some bounce dryer sheets and run 
over my cloths and hair This usually works.
Kim D




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] anti static spray?

2012-02-10 Thread jstaruk
We have a humidifier going most of the time.  This seems to alleviate the
static problems commonly experienced during the dry winter months.

Jim

___
James E. Staruk HT(ASCP)
 www.masshistology.com
   www.nehorselabs.com
 
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Friday, February 10, 2012 3:45 PM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] anti static spray?

We also have problems with static in our student lab.  We soak paper towels
with water and keep them in the waste tray.  Each time the student skims the
water bath with a Kim wipe they also add that wet towel to the waste tray.
The moisture really helps.





Elizabeth Chlipala  Sent by:
histonet-boun...@lists.utsouthwestern.edu
02/10/2012 11:48 AM

To
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] anti static spray?






Happy Friday Everyone

I need to purchase a product called static guard or static x  to help with
static when sectioning.  Can I just purchase this from a regular store or a
histology vendor?  Actually if anyone has any advice or suggestions on how
to deal with static when sectioning that would be great.

Thanks so much in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO
Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-
No virus found in this message.
Checked by AVG - www.avg.com
Version: 2012.0.1913 / Virus Database: 2112/4801 - Release Date: 02/10/12


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] AFB control

2012-02-10 Thread Debra Siena
It is also a requirement if you are doing work in or for the state of New York. 
 

Debbie Siena
800.442.3573 ext. 229 | www.statlab.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Friday, February 10, 2012 2:49 PM
To: Jennifer MacDonald; Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] AFB control

Recommended by who?
Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Friday, February 10, 2012 2:43 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; 
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB control


It is recommended to run a negative AFB control to rule out false 
positives.  Some sources of water have acid fast organisms, not TB, and if 
the water is used in the water baths or for staining you could pick up 
those organisms.  The recommendation is to cut a section of tissue, known 
NOT to contain AFB organisms, and run it through with the positive control 
and patient.  You would pick it up on the same water bath that you used 
for the patient.




Rene J Buesa  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/10/2012 11:55 AM

To
histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 

cc

Subject
Re: [Histonet] AFB control






All I recall about controls in HC procedures (any of them) is to have a 
(+) control. The only one that requires both (-) and (+) is PAS René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker  
wrote:


From: Stephanie Hoyle-Thacker 
Subject: [Histonet] AFB control
To: histonet@lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a 
regulation that states negative controls should be run for AFB stains.  I 
seem to recall seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker   
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] AFB control

2012-02-10 Thread Jennifer MacDonald
Carson




"Mike Pence"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/10/2012 12:49 PM

To
"Jennifer MacDonald" , "Rene J Buesa" 

cc
histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 
, histonet-boun...@lists.utsouthwestern.edu
Subject
RE: [Histonet] AFB control






Recommended by who?
Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Friday, February 10, 2012 2:43 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; 
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB control


It is recommended to run a negative AFB control to rule out false 
positives.  Some sources of water have acid fast organisms, not TB, and if 

the water is used in the water baths or for staining you could pick up 
those organisms.  The recommendation is to cut a section of tissue, known 
NOT to contain AFB organisms, and run it through with the positive control 

and patient.  You would pick it up on the same water bath that you used 
for the patient.




Rene J Buesa  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/10/2012 11:55 AM

To
histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 

cc

Subject
Re: [Histonet] AFB control






All I recall about controls in HC procedures (any of them) is to have a 
(+) control. The only one that requires both (-) and (+) is PAS René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker  
wrote:


From: Stephanie Hoyle-Thacker 
Subject: [Histonet] AFB control
To: histonet@lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a 
regulation that states negative controls should be run for AFB stains.  I 
seem to recall seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] AFB control

2012-02-10 Thread Mike Pence
Recommended by who?
Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Friday, February 10, 2012 2:43 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; Stephanie Hoyle-Thacker; 
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB control


It is recommended to run a negative AFB control to rule out false 
positives.  Some sources of water have acid fast organisms, not TB, and if 
the water is used in the water baths or for staining you could pick up 
those organisms.  The recommendation is to cut a section of tissue, known 
NOT to contain AFB organisms, and run it through with the positive control 
and patient.  You would pick it up on the same water bath that you used 
for the patient.




Rene J Buesa  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/10/2012 11:55 AM

To
histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 

cc

Subject
Re: [Histonet] AFB control






All I recall about controls in HC procedures (any of them) is to have a 
(+) control. The only one that requires both (-) and (+) is PAS René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker  
wrote:


From: Stephanie Hoyle-Thacker 
Subject: [Histonet] AFB control
To: histonet@lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a 
regulation that states negative controls should be run for AFB stains.  I 
seem to recall seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker   
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] RE: Hemisecting a fresh-frozen brain

2012-02-10 Thread Hilary Smith
Thanks Brett - I was hoping that someone out there had done this.  If I screw 
up the cerebellum and brainstem it is no big deal as we are only looking at 
forebrain structures. This is very encouraging!

Thanks again,
Hilary

-Original Message-
From: Connolly, Brett M [mailto:brett_conno...@merck.com] 
Sent: Friday, February 10, 2012 3:40 PM
To: Hilary Smith; histonet@lists.utsouthwestern.edu
Subject: RE: Hemisecting a fresh-frozen brain

Hilary,

I have done this before.

Definitely warm to -20C (overnight) otherwise it will likely fracture. A 
non-serrated brain slicing autopsy knife works well...it is best to use a knife 
that has a big blade and a sturdy handle. You will need a little bit of 
downward pressure on the knife and a slow, short sawing motion to work your way 
through. Cutting through the cerebral midline won't be much of a problem, be 
careful with the cerebellum and brainstem. Too much pressure and cutting too 
fast is bad.

Good luck,
Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilary Smith
Sent: Friday, February 10, 2012 3:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hemisecting a fresh-frozen brain

Hi everyone

I need to sagitally hemisect a fresh-frozen rhesus brain that has been stored 
at -80°C .  Does anyone have any advice on the best way to go about this?  I 
imagine warming it to about -20°C to reduce brittleness might be a good idea 
but I am not sure what my weapon of choice should be.  Can't use anything that 
will generate heat of course.  Will a non-serrated blade such as a tissue 
slicer blade do the trick at -20°C?  Or do I need to go to something like an 
ultrafine wire saw?  Or ultrafine hacksaw perhaps?  I welcome even the most 
outlandish suggestions!

Thanks,

Hilary

Hilary Smith
Research Assistant
Neuroimaging Lab
Department of Physiology and Pharmacology
Wake Forest School of Medicine
Medical Center Boulevard
Winston-Salem,  NC 27157
Phone 336.716.8649
Fax 336.716.8689

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Question - Pancreatic CA Research

2012-02-10 Thread Tunde Ajibade

Visit the website below

http://www.cancer.gov/cancertopics/types/pancreatic


 Tunde Ajibade BS, HTL(ASCP)QIHC
 Histology Supervisor
 Medical Center Hospital
 Odessa,TX
 Tel:432-640-2348
 Fax:432-640-2303
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Friday, February 10, 2012 12:49 PM
To: Histonet
Subject: [Histonet] Question - Pancreatic CA Research

We have a patient with pancreatic cancer that is close to death.  The patient 
has requested that her body be donated for pancreatic cancer research.  Is 
there anyone in the Histonet Community that is working in pancreatic cancer 
research that can help me with this patient's request?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



CONFIDENTIALITY NOTICE: The documents accompanying this email transmission 
contain confidential information belonging to the sender that is legally 
privileged. This information is intended only for the use of the individual or 
entity named above. The authorized recipient of this information is prohibited 
from disclosing this information to any other party and is required to destroy 
the information after its stated need has been fulfilled.  If you are not the 
intended recipient, you are hereby notified that any disclosure, copying, 
distribution, or action taken in reliance on the contents of these documents is 
strictly prohibited. If you have received this email in error, please notify 
the sender immediately to arrange for return of these documents.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] anti static spray?

2012-02-10 Thread Jennifer MacDonald
We also have problems with static in our student lab.  We soak paper 
towels with water and keep them in the waste tray.  Each time the student 
skims the water bath with a Kim wipe they also add that wet towel to the 
waste tray.  The moisture really helps.




Elizabeth Chlipala  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/10/2012 11:48 AM

To
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] anti static spray?






Happy Friday Everyone

I need to purchase a product called static guard or static x  to help with 
static when sectioning.  Can I just purchase this from a regular store or 
a histology vendor?  Actually if anyone has any advice or suggestions on 
how to deal with static when sectioning that would be great.

Thanks so much in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] AFB control

2012-02-10 Thread Jennifer MacDonald
It is recommended to run a negative AFB control to rule out false 
positives.  Some sources of water have acid fast organisms, not TB, and if 
the water is used in the water baths or for staining you could pick up 
those organisms.  The recommendation is to cut a section of tissue, known 
NOT to contain AFB organisms, and run it through with the positive control 
and patient.  You would pick it up on the same water bath that you used 
for the patient.




Rene J Buesa  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/10/2012 11:55 AM

To
histonet@lists.utsouthwestern.edu, Stephanie Hoyle-Thacker 

cc

Subject
Re: [Histonet] AFB control






All I recall about controls in HC procedures (any of them) is to have a 
(+) control. The only one that requires both (-) and (+) is PAS
René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker  
wrote:


From: Stephanie Hoyle-Thacker 
Subject: [Histonet] AFB control
To: histonet@lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a 
regulation that states negative controls should be run for AFB stains.  I 
seem to recall seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker   
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] RE: Hemisecting a fresh-frozen brain

2012-02-10 Thread Connolly, Brett M
Hilary,

I have done this before.

Definitely warm to -20C (overnight) otherwise it will likely fracture. A 
non-serrated brain slicing autopsy knife works well...it is best to use a knife 
that has a big blade and a sturdy handle. You will need a little bit of 
downward pressure on the knife and a slow, short sawing motion to work your way 
through. Cutting through the cerebral midline won't be much of a problem, be 
careful with the cerebellum and brainstem. Too much pressure and cutting too 
fast is bad.

Good luck,
Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilary Smith
Sent: Friday, February 10, 2012 3:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hemisecting a fresh-frozen brain

Hi everyone

I need to sagitally hemisect a fresh-frozen rhesus brain that has been stored 
at -80°C .  Does anyone have any advice on the best way to go about this?  I 
imagine warming it to about -20°C to reduce brittleness might be a good idea 
but I am not sure what my weapon of choice should be.  Can't use anything that 
will generate heat of course.  Will a non-serrated blade such as a tissue 
slicer blade do the trick at -20°C?  Or do I need to go to something like an 
ultrafine wire saw?  Or ultrafine hacksaw perhaps?  I welcome even the most 
outlandish suggestions!

Thanks,

Hilary

Hilary Smith
Research Assistant
Neuroimaging Lab
Department of Physiology and Pharmacology
Wake Forest School of Medicine
Medical Center Boulevard
Winston-Salem,  NC 27157
Phone 336.716.8649
Fax 336.716.8689

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] anti static spray?

2012-02-10 Thread Houston, Ronald
Liz,
we bought anti-static mats that are grounded and placed underneath the 
microtomes. This helped a lot, although we do have an anti-static brush and 
spray that we use in extreme circumstances when the air handlers go down.

I don't have the information to hand just now, but if you are ineterested I can 
get it to you on Monday

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Friday, February 10, 2012 3:15 PM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] anti static spray?

I have this problem a lot. I have static so bad literally blue sparks fly from 
my finger tips. I'm scared I'm going to blow up something one day and I walk 
around terrified to open doors. Anyway, in order to remedy my problem during 
sectioning I use a lot of hand lotion and get some bounce dryer sheets and run 
over my cloths and hair This usually works. 
Kim D

Sent from my iPhone

On Feb 10, 2012, at 2:46 PM, Elizabeth Chlipala  wrote:

> Happy Friday Everyone
> 
> I need to purchase a product called static guard or static x  to help with 
> static when sectioning.  Can I just purchase this from a regular store or a 
> histology vendor?  Actually if anyone has any advice or suggestions on how to 
> deal with static when sectioning that would be great.
> 
> Thanks so much in advance
> 
> Liz
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, 
> LLC PO Box 18592 Boulder, CO 80308-1592
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> www.premierlab.com
> 
> Ship to address:
> 
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
intended recipient (or authorized to receive information for the
intended recipient), you are hereby notified that any review, use,
disclosure, distribution, copying, printing, or action taken in
reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] anti static spray?

2012-02-10 Thread Elizabeth Chlipala
Thanks

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Kim Donadio [mailto:one_angel_sec...@yahoo.com]
Sent: Friday, February 10, 2012 1:15 PM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] anti static spray?

I have this problem a lot. I have static so bad literally blue sparks fly from 
my finger tips. I'm scared I'm going to blow up something one day and I walk 
around terrified to open doors. Anyway, in order to remedy my problem during 
sectioning I use a lot of hand lotion and get some bounce dryer sheets and run 
over my cloths and hair This usually works.
Kim D

Sent from my iPhone

On Feb 10, 2012, at 2:46 PM, Elizabeth Chlipala  wrote:

> Happy Friday Everyone
>
> I need to purchase a product called static guard or static x  to help with 
> static when sectioning.  Can I just purchase this from a regular store or a 
> histology vendor?  Actually if anyone has any advice or suggestions on how to 
> deal with static when sectioning that would be great.
>
> Thanks so much in advance
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308-1592
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> www.premierlab.com
>
> Ship to address:
>
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] anti static spray?

2012-02-10 Thread Kim Donadio
I have this problem a lot. I have static so bad literally blue sparks fly from 
my finger tips. I'm scared I'm going to blow up something one day and I walk 
around terrified to open doors. Anyway, in order to remedy my problem during 
sectioning I use a lot of hand lotion and get some bounce dryer sheets and run 
over my cloths and hair This usually works. 
Kim D

Sent from my iPhone

On Feb 10, 2012, at 2:46 PM, Elizabeth Chlipala  wrote:

> Happy Friday Everyone
> 
> I need to purchase a product called static guard or static x  to help with 
> static when sectioning.  Can I just purchase this from a regular store or a 
> histology vendor?  Actually if anyone has any advice or suggestions on how to 
> deal with static when sectioning that would be great.
> 
> Thanks so much in advance
> 
> Liz
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308-1592
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> www.premierlab.com
> 
> Ship to address:
> 
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Hemisecting a fresh-frozen brain

2012-02-10 Thread Hilary Smith
Hi everyone

I need to sagitally hemisect a fresh-frozen rhesus brain that has been stored 
at -80°C .  Does anyone have any advice on the best way to go about this?  I 
imagine warming it to about -20°C to reduce brittleness might be a good idea 
but I am not sure what my weapon of choice should be.  Can't use anything that 
will generate heat of course.  Will a non-serrated blade such as a tissue 
slicer blade do the trick at -20°C?  Or do I need to go to something like an 
ultrafine wire saw?  Or ultrafine hacksaw perhaps?  I welcome even the most 
outlandish suggestions!

Thanks,

Hilary

Hilary Smith
Research Assistant
Neuroimaging Lab
Department of Physiology and Pharmacology
Wake Forest School of Medicine
Medical Center Boulevard
Winston-Salem,  NC 27157
Phone 336.716.8649
Fax 336.716.8689

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] RE: anti static spray?

2012-02-10 Thread Rathborne, Toni
I remember buying this product years ago, before dryer sheets were invented. At 
the time, you could buy it in any grocery store or WalMart. 
What I would suggest is for you to check your humidity in the room. Quite often 
static is caused by low humidity levels. If possible, use a humidifier, or 
steamer if you have one. This should add more humidity to the room and help 
alleviate the problem.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Friday, February 10, 2012 2:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] anti static spray?

Happy Friday Everyone

I need to purchase a product called static guard or static x  to help with 
static when sectioning.  Can I just purchase this from a regular store or a 
histology vendor?  Actually if anyone has any advice or suggestions on how to 
deal with static when sectioning that would be great.

Thanks so much in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 
18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] anti static spray?

2012-02-10 Thread Rene J Buesa
Probably the histology vendor is selling you the same brand product you can buy 
in a store, but with a different "proprietary" label.
René J.

--- On Fri, 2/10/12, Elizabeth Chlipala  wrote:


From: Elizabeth Chlipala 
Subject: [Histonet] anti static spray?
To: "histonet@lists.utsouthwestern.edu" 
Date: Friday, February 10, 2012, 2:46 PM


Happy Friday Everyone

I need to purchase a product called static guard or static x  to help with 
static when sectioning.  Can I just purchase this from a regular store or a 
histology vendor?  Actually if anyone has any advice or suggestions on how to 
deal with static when sectioning that would be great.

Thanks so much in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] AFB control

2012-02-10 Thread Rene J Buesa
All I recall about controls in HC procedures (any of them) is to have a (+) 
control. The only one that requires both (-) and (+) is PAS
René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker  wrote:


From: Stephanie Hoyle-Thacker 
Subject: [Histonet] AFB control
To: histonet@lists.utsouthwestern.edu
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a regulation 
that states negative controls should be run for AFB stains.  I seem to recall 
seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker               
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] anti static spray?

2012-02-10 Thread Elizabeth Chlipala
Happy Friday Everyone

I need to purchase a product called static guard or static x  to help with 
static when sectioning.  Can I just purchase this from a regular store or a 
histology vendor?  Actually if anyone has any advice or suggestions on how to 
deal with static when sectioning that would be great.

Thanks so much in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] AFB control

2012-02-10 Thread Stephanie Hoyle-Thacker

Is anyone using a negative control for AFB stains?  If so is there a regulation 
that states negative controls should be run for AFB stains.  I seem to recall 
seeing such a regulation but cannot find it.
 
Thanks for your help!
 
Renee Thacker 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Question - Pancreatic CA Research

2012-02-10 Thread Richard Cartun
We have a patient with pancreatic cancer that is close to death.  The patient 
has requested that her body be donated for pancreatic cancer research.  Is 
there anyone in the Histonet Community that is working in pancreatic cancer 
research that can help me with this patient's request?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Antibody

2012-02-10 Thread McMahon, Loralee A
Looking for either IVD or ASR antibodies for

MDM 2 and CDK4.

Vendors ok to respond.

Thank you.



Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] PA area folks--company to NOT use

2012-02-10 Thread Emily Sours
Take it with a grain of salt, it's from a character on Parks and Rec.  He's
supposed to be over the top, outrageously libertarian.  It's a good tv
show, watch it on hulu for free.

Emily


The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson



On Thu, Feb 9, 2012 at 4:20 PM, Davide Costanzo wrote:

> Surely, as healthcare professionals, we can derive a better example of
> American Freedom" than obesity and gluttony.
>
> Am I the only one offended by Mr. Swanson's quote being used in a forum
> for medical professionals? Just curious.
>
>
> Sent from my Windows Phone
> From: Rene J Buesa
> Sent: 2/4/2012 9:12 AM
> To: histonet@lists.utsouthwestern.edu; Emily Sours
> Subject: Re: [Histonet] PA area folks--company to NOT use
> You should also file a complaint with the Better Business Bureau of your
> area.
> René J.
>
> --- On Fri, 2/3/12, Emily Sours  wrote:
>
>
> From: Emily Sours 
> Subject: [Histonet] PA area folks--company to NOT use
> To: histonet@lists.utsouthwestern.edu
> Date: Friday, February 3, 2012, 4:12 PM
>
>
> Hello histonetters!
>
> I am having a great deal of trouble getting a microscope cleaning bill
> paid.  We were overcharged by an hour.  I suggest if you ever need your
> instruments cleaned or repaired, DO NOT USE GEORGE NABLE INSTRUMENTS.  He
> has consistently overcharged us for his work, even after writing down a
> certain price on a purchase order.
> Just a warning.  He works in the Pittsburgh area.
> I wish there was a yelp for scientists where I could post this, because I
> know  a lot of people use him.
>
>
> Emily  Sours
>
>
> The whole point of this country is if you want to eat garbage, balloon up
> to 600 pounds and die of a heart attack at 43, you can! You are free to do
> so. To me, that’s beautiful.
> --Ron Swanson
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Job in AZ

2012-02-10 Thread Shirley A. Powell
Asked to pass this on.
Opportunity in AZ

Full time Histotech opportunity w/Cancer Treatment Centers of America in 
Goodyear, AZ?  This is a great opportunity offering an annual bonus, 
competitive pay and full (attractive) benefits.
Requires Bachelor's Degree in Biological/Physical Sciences or equivalent; 
minimum of 2-5 yrs experience as a Histotechnologist and ASCP registration 
preferred.
A national network of hospitals providing a comprehensive, fully integrative 
approach to cancer care. CTCA serves patients with advanced cancer from all 50 
states at facilities located in suburban Chicago, Philadelphia, Tulsa and 
suburban Phoenix. CTCA offers state-of-the-art oncology treatments combined 
with complementary therapies to treat the disease and help improve each 
person's quality of life.

CTCA is committed to becoming the best place to work. One way that we strive to 
achieve this goal is by offering a full spectrum of benefits which include: 
medical, dental, Healthy Awards Program, vision, flexible spending accounts, 
basic life and AD&D Insurance, supplemental life and AD&D insurance, short and 
long-term disability insurance, long-term care insurance, supplemental 
voluntary benefits, 401(k) savings and retirement plan, stakeholder assistance 
programs, adoption assistance, nutritional supplements, educational benefits, 
paid time off and an attractive incentive compensation program.

To learn more about this opportunity and to apply online, click on the link:  
http://bit.ly/ypjhC8  (Histotech/Lab)

Any question, you may call me directly at (651) 769-4067. Thank you.

Liz Bornhofen
Sr. Clinical Recruiter
Cancer Treatment Centers of America
(651) 769-4067
lbornho...@cor3talent.com

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] (no subject)

2012-02-10 Thread Segovia, Claudia


Claudia N. Segovia
Neurohistologist
NeuroScience Associates
10915 Lake Ridge Drive
Knoxville, TN  37934
865-675-2245
csego...@nsalabs.com

STATEMENT OF CONFIDENTIALITY:
The information contained in this electronic message and any attachments are 
intended for the exclusive use of the addressee(s) and may contain confidential 
or privileged information. If you are not the intended recipient, or the person 
responsible for delivering email to the intended recipient, be advised you have 
received this message in error and any use, dissemination, forwarding, printing 
or copying is strictly prohibited. Please notify NeuroScience Associates 
immediately at 865-675-2245 or at csego...@nsalabs.com, and destroy all copies 
of this message and any attachments. You will be reimbursed for reasonable 
costs incurred in notifying us.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet