[Histonet] CPT Code

[Marcia Fisher]

88365 x 2:  Kappa/Lambda by ISH   Does this code qualify for charges for bone 
marrows, fresh tissue and/or paraffin embedded?  Thank you.  

Marcia Fisher
Histology Supervisor/Lab Safety Officer
El Centro Regional Medical Center
 
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From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Sunday, February 12, 2012 10:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 99, Issue 16

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Today's Topics:

   1. Formalin training (Ann Angelo)
   2. Anti Static Guns (Marcia Fisher)
   3. Invitation from HistoTALK (David Kemler)


--

Message: 1
Date: Sat, 11 Feb 2012 13:20:04 -0500 (EST)
From: Ann Angelo 
Subject: [Histonet] Formalin training
To: histonet@lists.utsouthwestern.edu
Message-ID: <8ceb700e9485795-1a90-4...@webmail-m165.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Does anyone have a training program/presentation that can be used to train 
employees about 10% NBF in the Histology lab?
Ann


--

Message: 2
Date: Sat, 11 Feb 2012 18:29:06 +
From: Marcia Fisher 
Subject: [Histonet] Anti Static Guns
To: "histonet@lists.utsouthwestern.edu"

Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

We use ZeroStat Antistatic Guns in our lab.  Originally designed to remove fine 
dust particles from vinyl records (remember those?), I first began using them 
back in the 80's in our EM lab.  When I arrived here 3 years ago and living in 
the desert where we have virtually no humidity, especially right now, I brought 
in my own personal gun (yes, I still have vinyls!) and introduced it to the 
lab.  This gun is way over 20 years old and still works.  If you google these, 
you will find them out there. But search around for the best price.  You used 
to be able to walk in to a record store and buy them for about $10 but those 
stores are now obsolete too.  I found some good prices on eBay.  No chemicals, 
no harm to the environment, no smell, no mess!  

Marcia Fisher
Histology Supervisor/Lab Safety Officer
El Centro Regional Medical Center
?
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Sent: Saturday, February 11, 2012 10:03 AM
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Today's Topics:

   1. Re: anti static spray (Rick Tiefenauer)


--

Message: 1
Date: Sat, 11 Feb 2012 08:53:35 -0800
From: Rick Tiefenauer 
Subject: Re: [Histonet] anti static spray
To: l...@premierlab.com
Cc: Histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset=ISO-8859-1

Liz

During my Navy days to help prevent static problems off X-ray films, we
used a 95(ish)% isopropyl and water mix in a squeeze spray bottle.  A very
light mist before opening the paper film holders, and allowing it to
completely evaporate, normally did the 

RE: [Histonet] Ventana xt

[Burton, Lynn]

One great thing is that it does everything on the machine. It deparrifinizes as 
well as stains. The ultra view platform has even simplified the steps from 
machine to coverslipping. It can be set to run overnight. It has delayed timers 
for starting or ending. 
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of del phillips 
[dphill...@vetmed.lsu.edu]
Sent: Thursday, February 16, 2012 12:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana xt

Looking for any pros and cons on the Ventana XT.



Thanks

Del

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[Histonet] Ventana XT Closed System misnomer

[Gagnon, Eric]

Responding to Del who asked about pros/cons of Ventana BenchMark XT:

 

Ventana is often equated with 'closed system'.  This is a misnomer.  The
only thing I regard as closed (besides some colleagues' minds on this
issue) is the detection system kit and bulk reagents that are used on
the instrument.  While these are proprietary, they are also of extremely
high quality, resiliency, consistency, and in my opinion, dependability.
Preparing one's own reagents can lead to a lack of consistency of
staining, and we have confidence in Ventana reagents.

 

As others have correctly noted...other non-Ventana antibodies can be
used in Ventana dispensers.  We use an equal mix of ready-to-use
antibodies with our own diluted antibodies from other suppliers.  How is
this a closed system? 

 

Pre-treatments can also be used in Ventana dispensers. There is a range
of non-ab reagents such as hematoxylins and proteases.  There are a
range of detection system kits as well - at least four that I can think
of.

 

Perhaps this sounds like my personal crusade, as a Ventana user;  to try
to demolish the 'closed system' myth.  In my professional interactions
with non-Ventana users, this is often heard as a derogatory, negative
reaction to Ventana, which I certainly speak truth to at every
opportunity.

 

Del, we have found the BenchMark XT's very reliable, with good support
and service, plus excellent quality reagents including antibodies.  

 

Eric Gagnon MLT

Histology Laboratory

Kingston General Hospital,

Kingson, Ontario, Canada

 

 

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RE: [Histonet] Ventana xt

[CHRISTIE GOWAN]

Pros:
Lends itself well to labs that do basic IHC staining
Antibodies are pre-diluted so no guess work on dilutions
Good for batching runs
Consistent quality of slides
24 hour technical support
Can run molecular probes
protocols are easy to adjust
 
Cons:
Closed system
Must use Ventana antibodies or purchase special dispensers if using non Ventana 
products
Pre-dilute antibodies are pricey
Stand alone instrument so must have space for it
 
I'm sure there is more but you just really need to see what your needs are. We 
have the XT and Ultra as well as open systems. We love the Ventana's but we 
also will always have open platforms because we do a lot of research. The work 
flow is another thing you need to look at. How many slides do you turn out in 
one day? What is your turn around time? The XT is a great instrument but it 
depends on your lab. Hope this helps.
Christie Gowan
UAB Hospital
 
 

> From: dphill...@vetmed.lsu.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 16 Feb 2012 12:29:37 -0600
> Subject: [Histonet] Ventana xt
> 
> Looking for any pros and cons on the Ventana XT.
> 
> 
> 
> Thanks
> 
> Del
> 
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RE: [Histonet] Meditech users - pathologists ordering levels

[Rathborne, Toni]

Only occasionally.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, February 16, 2012 2:19 PM
To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Meditech users - pathologists ordering levels

I have never seen any pathologist I have worked for look at a block before 
asking for recuts.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Wednesday, February 15, 2012 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Meditech users - pathologists ordering levels

Sheila asks:

>>Hello: Are any of the Meditech users having the pathologists order 
>>their levels in the computer? We would like to implement this 
>>procedure. Any suggestions?<<

Never seen this - but it sounds like a nifty little time-waster for all 
concerned.

I usually examine the paraffin block before ordering levels or other recuts - 
to make sure the block isn't exhausted or horrible. Am I the only pathologist 
who does this?

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] Meditech users - pathologists ordering levels

[Horn, Hazel V]

I have never seen any pathologist I have worked for look at a block before 
asking for recuts.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Wednesday, February 15, 2012 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Meditech users - pathologists ordering levels

Sheila asks:

>>Hello: Are any of the Meditech users having the pathologists order 
>>their levels in the computer? We would like to implement this 
>>procedure. Any suggestions?<<

Never seen this - but it sounds like a nifty little time-waster for all 
concerned.

I usually examine the paraffin block before ordering levels or other recuts - 
to make sure the block isn't exhausted or horrible. Am I the only pathologist 
who does this?

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] Jones/PAMS

[Elizabeth Cameron]

Hi,
I was wondering if anyone has any suggestions for a Jones/PAMS stain that is 
not working properly. This is something we don't do often.  The last time we 
did it was a year and a half ago, and it seemed fine at the time.
We have tried 3 or 4 protocols, including an ammoniacal silver, and it is still 
not working properly.  In some protocols, our red cells are staining but the 
capillaries in the glomeruli do not seem to be picking up the silver.  In other 
protocols, there are nuclei of some cells that should not be staining that are, 
but again, the capillaries are not.  We are working on mouse tissue that is 
fixed in NBF.  The strange thing is the stain seems to be working well on 
Bouins and Telly's fixed tissue.  I even tried mordanting in Bouins!  We have 
tried multiple kidneys with the same results.  We are on new bottles of silver 
and periodic acid, although our methenamine has been around a while.  Any 
suggestions would be greatly appreciated.
Thanks!
Liz
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[Histonet] Myelin staining of fresh-frozen tissue

[Hilary Smith]

Hi Histonetters

Has anyone had success with Weil's myelin stain in fresh-frozen tissue?  And 
what is my best bet for post-fixation prior to staining?  Will 4% 
paraformaldehyde do the trick?  Presumably for a myelin stain I shouldn't defat?

Thanks,

Hilary

Hilary Smith
Research Assistant
Porrino Neuroimaging Lab
Department of Physiology and Pharmacology
Wake Forest School of Medicine
Medical Center Boulevard
Winston-Salem,  NC 27157
Phone 336.716.8649
Fax 336.716.8689

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[Histonet] RE: Opinions on Leica stainer

[Rathborne, Toni]

We love our ST5020. The ovens are a great feature. Very flexible. Leica service 
has been very good, especially this past year - I think they made some changes 
to their dispatch. Consumables will depend on who you get your reagents from. 
We have the CV5030 coverslipper attached, so the "load it and walk away" 
feature is nice to have.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Giracello, 
Darlene
Sent: Thursday, February 16, 2012 12:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opinions on Leica stainer


We would appreciate any opinions on the Leica Autostainer XL ST5010 and the 
ST5020.
Any likes, dislikes, has it been reliable? Consumables? Service?
We are a veterinary diagnostic lab, so flexibility is very important for us.

I bow to the wisdom of Histonet!!!

Thanks
Darlene
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[Histonet] Brucellosis

[Richard Cartun]

I have a consult (transplant kidney biopsy) that I believe is infected with 
Brucella.  My IHC test is positive, but I have never seen a human case before.  
Can someone familiar with this disease forward me a photo or a good reference 
that shows the typical morphology of this organism in human tissue?  Thank you.

Richard 

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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[Histonet] Ventana xt

[del phillips]

Looking for any pros and cons on the Ventana XT.

 

Thanks

Del

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[Histonet] RE: charging for muscle biopsy

[Weems, Joyce]

This is what our reference lab uses...


MUSCLE BX SDH -PATH REF 88319
MUSCLE BX COX -PATH REF 88319
MUSCLE BX ATP 4.3 -PATH REF 88319
MUSCLE BX ATP 9.4 -PATH REF 88319
MUSCLE BX CD8+T LYMPH -PATH REF 88342
MUSCLE BX STAIN MAJOR HISTO -PATH REF   88342
MUSCLE BX IP VIMENTIN -PATH REF 88342
MUSCLE BX NEURAL CELL ADHESION-PATH REF 88342
MUSCLE BX IP C5b 9 -PATH REF88342
MUSCLE BX CD79+B CELLS -PATH REF88342
MUSCLE BX CD68+MACROPHA-PATH REF88342
MUSCLE BX LEVEL IV - PATH   88305
MUSCLE BX CONGO RED - PATH  88313
MUSCLE BX TRICHROME - PATH  88314
MUSCLE BX OIL RED O - PATH  88314
MUSCLE BX PAS - PATH88314
MUSCLE BX ATP - 4.2 - PATH  88319
MUSCLE BX ATP - 10.4 - PATH 88319
MUSCLE BX NADH - PATH   88319
MUSCLE BX SDH - PATH88319
MUSCLE BX CYTOCHROME C - PATH   88319
MUSCLE BX COX/SDH - PATH88319
MUSCLE BX ACID PHOS - PATH  88319
ELECTRON MICROSCOPY -PATH REF   88348

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Thursday, February 16, 2012 12:57
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] charging for muscle biopsy
Importance: High

What CPT codes would you use for a muscle biopsy sample that was dissected for 
frozen enzyme histochemistry, paraffin and EM?

Thanks
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



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[Histonet] charging for muscle biopsy

[Houston, Ronald]

What CPT codes would you use for a muscle biopsy sample that was dissected for 
frozen enzyme histochemistry, paraffin and EM?

Thanks
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

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[Histonet] Opinions on Leica stainer

[Giracello, Darlene]

We would appreciate any opinions on the Leica Autostainer XL ST5010 and the 
ST5020.
Any likes, dislikes, has it been reliable? Consumables? Service?
We are a veterinary diagnostic lab, so flexibility is very important for us.

I bow to the wisdom of Histonet!!!

Thanks
Darlene
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[Histonet] Re:1

[Stephanie Hoyle-Thacker]

..Make alot of money using your PC  
http://misosofos.com/day.link.php?qositeid=66vy1
  
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[Histonet] (no subject)

[faith14913]

http://phamsp.org/wp-content/plugins/extended-comment-options/test.php?flag128.mp3

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[Histonet] RE: IHC and negative controls?

[Tom McNemar]

Thanks to everyone for the quick responses.

I am looking at ANP.22580 rev 12/12/2006 and it clearly talks about "a separate 
section of patient tissue..." but just a little further it talks about using 
internal negative controls ("The type of negative tissue control used (i.e., 
separate sections, internal controls or multitissue blocks) should be specified 
in the laboratory manual (refer to ANP.22250).")  I did not see anything useful 
in ANP.22250.

We are JCAHO accredited not CAP but I cannot find any JCAHO discussion on the 
subject.  Personally, I believe that we should run separate negative controls 
for each case.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, February 16, 2012 10:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC and negative controls?

Hi All,

We have one Ventana XT and often exceed the slide capacity.  Our pathologists 
no longer want us to run negatives and will use internal negatives instead.  Is 
this common practice?  Thanks.  I appreciate any feedback.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


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[Histonet] AUTO: Laura Miller is Out of the Office. (returning 04/16/2012)

[Laura . Miller]

I am out of the office until 04/16/2012.

I will be on medical leave until April 16, 2012. Please contact David
Archambault at 847-405-5017 if you need assistance.  Thank you.


Note: This is an automated response to your message  "Histonet Digest, Vol
99, Issue 20" sent on 2/16/2012 8:55:31 AM.

This is the only notification you will receive while this person is away.


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[Histonet] RE: IHC and negative controls?

[Settembre, Dana]

Tom,
As Tunde says, the Checklist 7/11/2011 question ANP.22570 does explain that you 
can do as your pathologists suggests, 
BUT there are rules to be followed if that is what you will do.

"A negative tissue control must be processed for each antibody in a given run. 
Any of the following can serve as a negative tissue control:
1. Multitissue blocks ...
2. The positive control slide or the patient test slides, if these slides 
contain tissue elements that should not react with the antibody.
[this is the one you want to follow]
3. A separate negative tissue control slide.

The type of negative tissue control used ... SHOULD BE SPECIFIED in the 
laboratory manual. ...

Evidence of Compliance:
Written procedure of the selection and use of ... tissue controls for IHC..."

Dana Settembre
University Hospital - UMDNJ
Newark, NJ

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tunde Ajibade
Sent: Thursday, February 16, 2012 10:40 AM
To: 'histonet@lists.utsouthwestern.edu'
Cc: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: [Histonet] RE: IHC and negative controls?

I am not sure if they can do that because CAP ANP.22570, explain this.

 Tunde Ajibade BS, HTL(ASCP)QIHC
 Histology Supervisor
 Medical Center Hospital
 Odessa,TX
 Tel:432-640-2348
 Fax:432-640-2303
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, February 16, 2012 9:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC and negative controls?

Hi All,

We have one Ventana XT and often exceed the slide capacity.  Our pathologists 
no longer want us to run negatives and will use internal negatives instead.  Is 
this common practice?  Thanks.  I appreciate any feedback.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


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[Histonet] RE: IHC and negative controls?

[Tunde Ajibade]

I am not sure if they can do that because CAP ANP.22570, explain this.

 Tunde Ajibade BS, HTL(ASCP)QIHC
 Histology Supervisor
 Medical Center Hospital
 Odessa,TX
 Tel:432-640-2348
 Fax:432-640-2303
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, February 16, 2012 9:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC and negative controls?

Hi All,

We have one Ventana XT and often exceed the slide capacity.  Our pathologists 
no longer want us to run negatives and will use internal negatives instead.  Is 
this common practice?  Thanks.  I appreciate any feedback.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


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[Histonet] tissue processors

[Bales, Candy A]

Good morning. These questions are on behalf of a friend who is in the market 
for a new tissue processor. She wants to know the pros and cons of the various 
processors in use.
She mentioned the Excelsior, VIP & Leica but is open to other suggestions. The 
workload is approximately 200 blocks or less per night. She is looking for 
something the doctors can easily stop and add cassettes to and restart.

She is also looking to replace her old linear stainer, no longer being produced 
by Thermo-Shandon.  Does anyone know of a company producing a linear stainer?
Or for those who have auto stainers, if you could give her the pros and cons of 
the models you use.

Thanking you in advance

Candy Bales, HT
Chief Histologist
The University  of Texas Health Science Center Houston-
School of Dentistry
Diagnostic & Biomedical Sciences
6516 M.D. Anderson Blvd. # 3.093
Houston, TX 77030
713.500.4411 office
713.500.4416 fax

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RE: [Histonet] Mice heads in Paraffin

[Thomas, Nancy]

Mote,
For the E17-19, we found that they need more time in paraffin.  We have 2 hours 
in each of the dehydration  and clearing steps and two hours in each of the 4 
paraffin baths.  This is 8 hours in paraffin, but for this larger size, that is 
what we found to work.

Nancy Thomas 
Stowers Institute for Medical Research
Kansas City, MO

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mote uncle
Sent: Thursday, February 16, 2012 8:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mice heads in Paraffin

Hello

This is my first post to this site. I have encountered a problem with embedding 
of mice heads in Paraffin (Gurr). Initially with Embryonic day 13-14, the 
protocol was working properly and the sections were also nice but with 
Embryonic day 17-19 I could see even after several hours of ethanol wash and 
xylene treatment the mice heads were soft but still I embedded the samples in 
paraffin. When I removed the paraffin blocks from the mold I could see the mice 
portion to be white, some flecks of wax coming out of surface and the surface 
of wax had some cracks. I could not rule out what was causing the problem.

Protocol I used for E 17-19

After Fixing in EDTA (2 weeks) and putting the mice heads in running water for 
48 hours,

50% Ethanol 3 hours

70% Ethanol 3 hours

95% Ethanol 3 hours

100% Ethanol 3 hours

100% Ethanol 3 hours

Xylene 2-4 hrs (till clearing of samples was observed)

Paraffin 30 mins

Paraffin 30 mins

Paraffin 30 mins

Paraffin 30 mins
Could anybody share me your protocol for Embryonic day 17-19 and Post natal 
days.
Regards
Mote
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[Histonet] IHC and negative controls?

[Tom McNemar]

Hi All,

We have one Ventana XT and often exceed the slide capacity.  Our pathologists 
no longer want us to run negatives and will use internal negatives instead.  Is 
this common practice?  Thanks.  I appreciate any feedback.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


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Re: [Histonet] (no subject)

[Richard Cartun]

Complete loss.  You can see patchy immunoreactivity in tumors with these 
markers. 

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Freeman, Carol"  2/16/2012 9:56 AM >>>


Good Morning Histonet,

Ok, We are just starting up with MSI testing in our facility and a
question has popped up about diagnosis of these slides...  Are you
looking for a COMPLETE LOSS of this protein in the tumor, or just a loss
of some expression.?  So if you have a normal piece of tissue staining
uniform and then a tumor of that same tissue showing vague patchy
staining in some cells and a loss in other cells of interest are you
considering that a loss or not.   Does the tumor have to show a COMPLETE
loss of this protein...  Any answer is appreciated.

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[Histonet] Interfacing CoPath with clients

[Houston, Ronald]

Does anyone have experience interfacing Sunquest CoPath with external clients 
so that they are able to access reports at will?

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org



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[Histonet] (no subject)

[Freeman, Carol]

 

Good Morning Histonet,

Ok, We are just starting up with MSI testing in our facility and a
question has popped up about diagnosis of these slides...  Are you
looking for a COMPLETE LOSS of this protein in the tumor, or just a loss
of some expression.?  So if you have a normal piece of tissue staining
uniform and then a tumor of that same tissue showing vague patchy
staining in some cells and a loss in other cells of interest are you
considering that a loss or not.   Does the tumor have to show a COMPLETE
loss of this protein...  Any answer is appreciated.

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[Histonet] Mice heads in Paraffin

[mote uncle]

Hello

This is my first post to this site. I have encountered a problem with
embedding of mice heads in Paraffin (Gurr). Initially with Embryonic day
13-14, the protocol was working properly and the sections were also nice
but with Embryonic day 17-19 I could see even after several hours of
ethanol wash and xylene treatment the mice heads were soft but still I
embedded the samples in paraffin. When I removed the paraffin blocks from
the mold I could see the mice portion to be white, some flecks of wax
coming out of surface and the surface of wax had some cracks. I could not
rule out what was causing the problem.

Protocol I used for E 17-19

After Fixing in EDTA (2 weeks) and putting the mice heads in running water
for 48 hours,

50% Ethanol 3 hours

70% Ethanol 3 hours

95% Ethanol 3 hours

100% Ethanol 3 hours

100% Ethanol 3 hours

Xylene 2-4 hrs (till clearing of samples was observed)

Paraffin 30 mins

Paraffin 30 mins

Paraffin 30 mins

Paraffin 30 mins
Could anybody share me your protocol for Embryonic day 17-19 and Post natal
days.
Regards
Mote
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RE: [Histonet] Ribbon won't stay on knife

[Rathborne, Toni]

I also find it helpful to blunt the bottom edge of the block by scraping to 
create two corners/angles on it instead of one 90 degree angle.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, February 16, 2012 6:16 AM
To: 'Jerry Ricks'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ribbon won't stay on knife

I use my forceps to round over the top edge of the block when this happens.  If 
you ever have trouble getting a ribbon to form because the sections do not 
stick together, you can also use the forceps to straighten the bottom edge of 
the block.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks
Sent: Wednesday, February 15, 2012 6:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ribbon won't stay on knife


Sometimes while cutting 5 micron paraffin sections...

I turn the wheel once and a nice section gets cut and lays down on my blade.  
When I turn the wheel again, as the knife holder is traveling Upwards, the top 
of my paraffin block will grab the ribbon and lift it off of the microtome 
blade, ruining my ribbon. Any ideas what is going on, how to fix it.

Today I'm using my old trust Spencer 820 microtome, but it also sometimes 
happens on my Leica RM2125.

Thanks,

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology


  
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[Histonet] New KOH-type stain

[Sally Price]

Histonetters:
The other day I recieved by mail a brochure for a stain in a one-gallon
bottle that claimed it stained fungal elements bluish-purple and
helped reading KOH's.  I gave the brochure to someone and they seem to have
misplaced it.  It wasn't the Chicago Sky Blue stain that's in those kits
(and is very expensive, 1 oz. bottles for $70).  Has anyone else received
this info and if so could they share the vendor/product info?
Cheers,
Sally
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RE: [Histonet] Ribbon won't stay on knife

[Tom McNemar]

I use my forceps to round over the top edge of the block when this happens.  If 
you ever have trouble getting a ribbon to form because the sections do not 
stick together, you can also use the forceps to straighten the bottom edge of 
the block.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks
Sent: Wednesday, February 15, 2012 6:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ribbon won't stay on knife


Sometimes while cutting 5 micron paraffin sections...

I turn the wheel once and a nice section gets cut and lays down on my blade.  
When I turn the wheel again, as the knife holder is traveling Upwards, the top 
of my paraffin block will grab the ribbon and lift it off of the microtome 
blade, ruining my ribbon. Any ideas what is going on, how to fix it.

Today I'm using my old trust Spencer 820 microtome, but it also sometimes 
happens on my Leica RM2125.

Thanks,

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology


  
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[Histonet] Thanks

[Marilyn Tyler]

Hi To All

A big thank you to everyone for their input regarding endogeous
peroxidase blocking.

Much appreciated

Marilyn



Medical School UCT
Dept of Surgery J50/26 or 30
Surgical research. OMB
Groote Schuur Hospital
Observatory
 
021-4066227
 




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