[Histonet] Does xylene cause skin cancer?
I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology CSF Cell Pellets made from Histogel
Hi, I haven't done a cellblock on CSF with Histogel, but I have has success with some fairly scanty cell culture specimens. A short processing cycle would be best. Try to make sure you have removed as much supernatant as possible to keep the gel from shriveling during processing. The IHC will be fine as long as the cells are suitably fixed ahead of time. Here is a link to the packaging insert for Histogel which describes preparation of cell blocks. https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf And here's a video of people using it for cytology specimens. http://tinyurl.com/7jb522e Amos On Tue, Feb 21, 2012 at 1:01 PM, wrote: > Message: 15 > Date: Tue, 21 Feb 2012 11:26:10 -0600 > From: "Turner, Leandra" > Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel > To: > Message-ID: ><3d79f47dc92b204f9e5d35c885dfc5cb010ab...@ausex2vs1.seton.org> > Content-Type: text/plain; charset="us-ascii" > > > > > > Hello Everyone, > >I am trying to find out a few of things about making cell pellets on > cerebrospinal fluids. I would like to know: > > > > 1. If anyone has ever made cell pellets from CSF's using Histogel > and has any tips or procedures they could share? > > > > 2. How to process the CSF pellets made with Histogel, do we need a > routine or stat process? (we use a Sakura Tissue Tek) > > > > 3. Can you do IHC staining on the pellets? > > > > > > Thank you for any and all help in advance. > > > > Leandra Turner, HT (ASPC)CM > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening in Orange county California
A part-time histotech position is open in our histology laboratory. Tuesday through Saturday starting at 5 am. If anyone is interested, please either fax or email me your resume. Thank you, Paula Lucas Bio-Path Medical Group Fountain Valley, CA 92708 Fax: 714-755-2984 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processor Question
Design flaw in the screen display. It is in the way of the chamber when opening chamber. If your not careful you will break the screen. Happens fairly often. Sent from my Windows Phone From: Gauch, Vicki Sent: 2/21/2012 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Question Hi everyone, We are in the market for new processors...and I was wondering if anyone could give me some pros and cons for the Tissue Tek VIP 6 tissue processor - how reliable are they? Ease of use ? Any known problems? Tissues process well? You knowall the usual questions we all ask for new equipment. Thanks in advance for your help, Vicki Gauch AMCH Albany, NY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking a Histology Supervisor in Connecticut
Slone Partners seeks a Histology Supervisor for our hospital based laboratory= client in Connecticut. The successful candidate= will have experience with workflow redesign and be great at managing change.= There are currently 38 people in the department, including 2 supervisors.= Lean and Six Sigma experience is a plus. Qualified= candidates will have a B.S. degree and either HT/HTL certification, with= supervisory experience, preferably in a busy, high-volume laboratory. Special features of this position:= The histology supervisor will have the opportunity to help redesign this= busy laboratory. If you meet these qualifications= and would like to be considered for this position, please submit your resume= to Darcy Bloch at dar...@slonepartners.com. If you= have experience in the diagnostic laboratory industry and wish to be considered= for other roles, please forward your resume to Tara Kochis at t...@slonepartners.com. All inquiries are kept confidential. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology CSF Cell Pellets made from Histogel
Hello Everyone, I am trying to find out a few of things about making cell pellets on cerebrospinal fluids. I would like to know: 1. If anyone has ever made cell pellets from CSF's using Histogel and has any tips or procedures they could share? 2. How to process the CSF pellets made with Histogel, do we need a routine or stat process? (we use a Sakura Tissue Tek) 3. Can you do IHC staining on the pellets? Thank you for any and all help in advance. Leandra Turner, HT (ASPC)CM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor Question
Hi everyone, We are in the market for new processors...and I was wondering if anyone could give me some pros and cons for the Tissue Tek VIP 6 tissue processor - how reliable are they? Ease of use ? Any known problems? Tissues process well? You knowall the usual questions we all ask for new equipment. Thanks in advance for your help, Vicki Gauch AMCH Albany, NY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] disposable blade system for microm 505e or
Hi netters, I have a system that uses full sized microtome blades for the Microm 505E cryostat. I think it would be cheaper to order the item I have seen before that us old timers used when disposable blade systems first came on the seen. The blade holder looks and feels like an old time microtome blade, but upon closer inspection is actually a holder for a disposable blade, then the entire "unit" fits into the microtome like the old blades did. So if I had one of those I could place that in the cryostat instead of using the entire assembly. I would prefer the entire assembly but I am as low budget as they get right now. Everything by hand(including ice bath, hot plates and paraffin dispensers for embedding). That will end soon though. Still hand processing, staining, coverslipping etc though. ANyone out there want to throw anything my way, sure would preeshiate it. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oil Red O
Oil Red O slides will never "fade". The technique isn't like typical histochemical procedures where dye molecules bind chemically to tissue components. In Oil Red O staining there isn't any chemical binding that can be reversed. What you end up with is a solution of the dye in the fat droplets. Once coverslipped with an aqueous mounting medium, the dye cannot escape from the fat droplets because it is insoluble in water. Therefore the dye in the fat droplets is just as stable as the dye solution in the bottle, and the slides will remain good as long as the coverslipping medium doesn't dry out - which in this case is the reason for refrigeration, to slow the drying of the mounting medium. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Position
Hello all, We have a histology position open. Requirements are HT certified or eligible, we are willing to train. Anyone interested please contact me with questions or you can apply at http://shoremedicalcenter.org/careers. Thanks! Jennifer Thawley HT, ASCP Histology Supervisor Shore Memorial Hospital (609) 653-3940 This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
