Re: [Histonet] While on the topic of harmful chemicals...
I would still use nitril gloves fir that. Nitril may be a little more expensive but for our department it's the only way to go if safety is practiced. Also I would recommend that you look at the Msds of all reagents you work with. Your department should gave a book. While we are on the subject all should be familiar with the chemicals we work with by looking at the Msds and the protocols which should include proper PPE. Our field of study is a dangerous field. We not only deal with biohazards of fresh tissue blood included we gave mass amounts of chemicals Be safe out there. And Claire I hope you feel better:) Kim :D On Feb 23, 2012, at 11:10 PM, "Ingles Claire " wrote: > I have been having some health issues lately (understatement) Does anyone > know the best formalin resistant disposable gloves? I gross (skin only) under > a fume hood but the gloves still come in contact with formalin naturally. I > am also (OK, mostly) interested in the health effects involving formalin, > Propar, and the alcohols as they pertain to the liver. (No guys, I mean > REAGENT alcohols, not the ingestable kind.) :) > Any direction I can be pointed in would be great. I am praying I won't have > to find a creative way of practicing my beloved career because these > chemicals are causing or making worse my health problems. > :( > > Claire > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] While on the topic of harmful chemicals...
I have been having some health issues lately (understatement) Does anyone know the best formalin resistant disposable gloves? I gross (skin only) under a fume hood but the gloves still come in contact with formalin naturally. I am also (OK, mostly) interested in the health effects involving formalin, Propar, and the alcohols as they pertain to the liver. (No guys, I mean REAGENT alcohols, not the ingestable kind.) :) Any direction I can be pointed in would be great. I am praying I won't have to find a creative way of practicing my beloved career because these chemicals are causing or making worse my health problems. :( Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Surgipath Millenium VSP3001/Leica Vogel 35396 Slide Writer
So apparently this model of slide writer is no longer manufactured, at least from what I can tell. My Leica rep says they don't make it anymore, but I really need to get my hands on one. Normally, I'm not a big fan of this printer for larger volume labs, but the way we make slides here at our immuno lab is perfectly conducive for the specific design of slide writer. I'm wondering if anyone out there in Histoland knows where I can get one new or used. Also, do you know if Leica still sells the ribbons that go with them? Finally, the ideal circumstance would be where we could demo one here; you know how it is getting the funding approved! However, one of our Pathologists has straight up said he'd buy it himself and worry about getting reimbursed for it later. Anyways, please let me know if you can help or point me in the right direction! Thanks, Drew Meyer, HT CSI Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block Shipping Question
Fed-Ex is good, but include a cold-pack in with the blocks. A common freezer pack from the local drug store will do it - and is reusable. FL From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor [b427...@aol.com] Sent: Thursday, February 23, 2012 4:23 PM To: hairlesstur...@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block Shipping Question FedEx overnight with a warning to keep from extreme temps. That should keep them off loading docks. -Original Message- From: E V To: histonet Sent: Thu, Feb 23, 2012 3:08 pm Subject: [Histonet] Paraffin Block Shipping Question Hi, am wanting to send out blocks to different sites across the country. My ain concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when onsidering the above stated concern? Thanks, __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Does xylene cause skin cancer?
As far as I know Xylene is not a conformed carcinogen. On the other hand the structure is close to that of Benzene which is a confirmed human carcinogen. It's an aromatic hydrocarbon, so why take chances. It makes sense to 1) minimize use. 2) Use fume hoods when possible. 3) Wear PPE--nitrile gloves not latex! http://en.wikipedia.org/wiki/Xylene http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/xylene/health_xyl.html http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/benzene/health_ben.html#_1_6 Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Thu, 23 Feb 2012 13:33:00 -0500 > From: rsrichm...@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Does xylene cause skin cancer? > > What's badly needed is a registry of death certificates of > histotechnologists. The thing I'd want to examine would be the > prevalence of myeloid leukemia and related diseases - known to be > elevated in workers exposed to benzene. If such a correlation were > found, it would mandate eliminating xylene from histology and cytology > labs, and increasing precautions for handling resinous mounting media. > > The American Medical Association maintains such a registry of American > physicians (or used to). It was used to establish that exposure to > ionizing radiation (fluoroscopes and other X-ray equipment) was > correlated with deaths from myeloid leukemia. Pathologists didn't have > any particular problems - here I'd wonder about formaldehyde exposure > and upper airway cancer. Pathologists get more exposure to > formaldehyde than do histotechnologists. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cytology slides
Hi every one, I would like to get people's opinions on whether mail-in cytology slides both stained and un-stained should be left out on the counter top or be put in a refrigerator until the next day for the tech to process them? We have always had a policy of leaving them in their cases at room temp. We now have a 'know-it-all' who is now working here and think he knows every thing. I would like a well educated summery to present to my Pathologist. Thanks so much, Fran ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cytokeratin Lu5
Hi Margaret, We have used CK-Lu5 in the past with great results. It stains dog, cat, pig, cow, horse, goat, deer, bird, frog, hedgehog, snake, guinea pig, rat, and bats so far. Use Proteinase digestion for antigen retrieval. We did notice that in recent times the staining intensity diminished with CK-Lu5 on some runs and we had to keep dropping the dilution down to more concentrated levels to achieve the same staining intensity as in the past. Dako's CK-MNF116 is also a great broad spectrum (also stains pretty much the same animals strongly). However, CK-Lu5 stains more hepatocytes than CK-MNF116 does, in my experience. Jan Shivers Section Head IHC/Histo/EM Veterinary Diagnostic Lab Univ. of Minnesota St. Paul, MN shive...@umn.edu On Thu, Feb 23, 2012 at 3:55 PM, Perry, Margaret wrote: > Has anyone used this antibody on dog? If so which company did you use? > Would you be willing to share your protocol? > > Margaret Perry HT(ASCP) > Dept of Veterinary and Biomedical services > Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cytokeratin Lu5
Has anyone used this antibody on dog? If so which company did you use? Would you be willing to share your protocol? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mAb to Serotonin
Does anyone have a good monoclonal antibody to Serotonin that they would recommend for diagnostic immunohistochemistry performed on formalin-fixed, paraffin-embedded human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block Shipping Question
FedEx overnight with a warning to keep from extreme temps. That should keep them off loading docks. -Original Message- From: E V To: histonet Sent: Thu, Feb 23, 2012 3:08 pm Subject: [Histonet] Paraffin Block Shipping Question Hi, am wanting to send out blocks to different sites across the country. My ain concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when onsidering the above stated concern? Thanks, __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Block Shipping Question
Hi, I am wanting to send out blocks to different sites across the country. My main concern is the block melting or being partially warped due to the heat. What is the most cost effective way of sending out numerous blocks when considering the above stated concern? Thanks, H ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Does xylene cause skin cancer?
-Original Message- From: Jackie O'Connor To: Jonathan.Cremer Sent: Thu, Feb 23, 2012 8:39 am Subject: Re: [Histonet] Does xylene cause skin cancer? I grew up in histology before MSDS's and OSHA. We practically bathed in xylene, which is no excuse for ignoring safety precautions now. In all my years and places I've worked, I don't know anyone who has developed cancer because of xylene exposure per se - you can all say "that they know of". I can honestly say, out of the hundreds of people I met and worked with, personally I only know of a few histotechs and pathologists who actually died from cancer over the last 40 years. I do, however, know a larger number of histotechs who have suffered unusual miscarriages, still births, and had children with unusual birth defects. That is something that no one (like the NSH or histology chemical providers) has really ever looked into. Common sense should always be your first line of defense, people.Don't get it on your skin if you can avoid contact, but if you're that paranoid about the chemicals you work with, you are probably in the wrong line of work. If you look at some of the special stains we work with, they are scarier than xylene. I'm jus' sayin . . . Jackie O' -Original Message- From: Jonathan Cremer To: Tony Henwood (SCHN) ; 'Hilary Smith' ; Debbie Faichney ; Rene J Buesa ; histonet ; Jenny Vega Sent: Thu, Feb 23, 2012 2:59 am Subject: RE: [Histonet] Does xylene cause skin cancer? But the gloves in that Ansell list are reusable, whereas I suppose that you mean he disposable Kimberley-Clark gloves? The thickness would be very different in hat case. And I wouldn't be able to mount a slide with thick, re-use gloves. :) In any case, the doctor for my professional yearly medical exam always tells me o only use nitrile gloves in combination with xylene, and that xylene will get hrough after a few minutes. So I just avoid getting xylene on my hands ltogether by using forceps to handle wet slides while coverslipping. -- onathan Cremer aboratory Technician astro-enterologie KUL CDG Bus 811; Labo Experimentele Immunologie; Herestraat 49; 3000 Leuven; elgium ___ an: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] amens Tony Henwood (SCHN) [tony.henw...@health.nsw.gov.au] erzonden: donderdag 23 februari 2012 1:56 an: 'Hilary Smith'; Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; enny Vega nderwerp: RE: [Histonet] Does xylene cause skin cancer? Wow, That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene, hereas Ansell's Nitrile gloves are (see http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) Regards ony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) aboratory Manager & Senior Scientist el: 612 9845 3306 ax: 612 9845 3318 he children's hospital at westmead nr Hawkesbury Road and Hainsworth Street, Westmead ocked Bag 4001, Westmead NSW 2145, AUSTRALIA Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Hilary Smith ent: Thursday, 23 February 2012 1:52 AM o: Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega ubject: RE: [Histonet] Does xylene cause skin cancer? You might want to go to something with even greater chemical resistance - thin itrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: "The substance may be toxic to blood, kidneys, iver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or rolonged exposure to the substance can produce target organs damage." I would definitely use gloves if I were you. Hilary Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Debbie Faichney ent: Wednesday, February 22, 2012 8:15 AM o: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega ubject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher hemical resistance than latex. I use them and change every 30 minutes to avoid reakthrough. Debbie Faichney nstitute of Aquaculture niversity of Stirling K -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Rene J Buesa ent: 22 February 2012 13:09 o: histonet@lists.utsouthwestern.edu; Jenny Vega ubject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, lthough dermatitis are well documented. egardless you should use gloves whenever your hands can get in contact with any hemical as a good safety practice. If your colleagues
[Histonet] Re: Does xylene cause skin cancer?
As I failed to mention in my earlier post - the problem with a registry is that many (most, in my personal experience) American histotechnologists are completely uncertified, and would not appear in any registry. One would hazard a guess that these uncertified techs get the most xylene exposure. Bob Richmond * On Thu, Feb 23, 2012 at 1:33 PM, Bob Richmond wrote: > What's badly needed is a registry of death certificates of > histotechnologists. The thing I'd want to examine would be the > prevalence of myeloid leukemia and related diseases - known to be > elevated in workers exposed to benzene. If such a correlation were > found, it would mandate eliminating xylene from histology and cytology > labs, and increasing precautions for handling resinous mounting media. > > The American Medical Association maintains such a registry of American > physicians (or used to). It was used to establish that exposure to > ionizing radiation (fluoroscopes and other X-ray equipment) was > correlated with deaths from myeloid leukemia. Pathologists didn't have > any particular problems - here I'd wonder about formaldehyde exposure > and upper airway cancer. Pathologists get more exposure to > formaldehyde than do histotechnologists. > > Bob Richmond > Samurai Pathologist > Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Adenovirus controls for paraffin sections
Hi, Can anyone recommend Adenovirus controls for paraffin sections. I can not find a vendor that sells these controls any longer. Any help would be appreciated. Pat Karlisch *E-Mail Confidentiality Notice* This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: does xylene cause skin cancer
Xylene is toxic and you should always wear gloves and a face mask when dealing with it. Dermal absorption is a lot slower than inhalation, but contact with organic solvents such as xylene is bad in general. With the issue of latex residue left on the slide, try using nitrile gloves. We use nitrile in our lab and we never have an issue with residue left on the slides. You may also want to wear two gloves on the hand that might come in contact with xylene because it can eat through gloves. I haven't found anything linking xylene as a human carcinogen, but it's toxic to the central nervous system as well as a known teratogen, so limiting any contact is advised. You can also use histoclear, another organic solvent that works really well that is far less toxic than xylene. Best, Rory On Feb 23, 2012, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to >histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at >histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Re: Histonet Digest, Vol 99, Issue 28 (Sheila Fonner) > 2. ADIPOPHILIN (Walls, Rachel) > 3. out of office (marilyn.a.we...@kp.org) > 4. RE: Does xylene cause skin cancer? (Mayer,Toysha N) > 5. Histology Lab Donations (Salim Yalcin Inan) > 6. Cleaning Bond Bulk Reagent Containers (Konop, Nicole) > > > -- > > Message: 1 > Date: Thu, 23 Feb 2012 06:44:25 -0500 > From: "Sheila Fonner" > Subject: RE: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > To: "'Madeleine Huey'" , > > Message-ID: <01ccf220$7e8ff2a0$7bafd7e0$@com> > Content-Type: text/plain;charset="iso-8859-1" > > ? You use regular superfrost plus slides for temperature verification of the > thermal pads? The slides to use are the superfrost plus slides from Ventana > that have a qc'd strip on them that will turn black if the temperatures of > pads are at acceptable levels. How do you prove that with a plain > superfrost plus slide? > > We use the suggested qc slides, but only do the test twice/yr instead of > quarterly. We re-wrote our procedure to indicate that this is what we do. > We then copy the slides onto paper and file for documentation purposes. You > could also keep the slides, if you have the room to file and store them. > CAP requires the written procedure as well as documentation that it has been > done. > > Sheila, HT (ASCP) > KDL Pathology > Knoxville, TN > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine > Huey > Sent: Wednesday, February 22, 2012 9:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > > Message: 15 > Date: Wed, 22 Feb 2012 15:26:43 + > From: Amber McKenzie > Subject: [Histonet] Temp verifier slides - Ventana equipment > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <5a33c952bb67f4468af1f36d739212bc115ec...@jerry.gia.com> > Content-Type: text/plain; charset="us-ascii" > > Do y'all run the temp verifier slides for the quarterly maintenance for the > Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 > months, esp if you have multiple pieces of equipment. > > Amber, > > We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just > fine. > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor - Pathology (IPOX & Histology) madelein...@elcaminohospital.org > > On Wed, Feb 22, 2012 at 10:01 AM, > wrote: >> Send Histonet mailing list submissions to >> � � � �histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> � � � �http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> � � � �histonet-requ...@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> � � � �histonet-ow...@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> � 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) >> � 2. RE: Processor Question (Davide Costanzo) >> � 3. Job Opening in Orange county California (Paula Lucas) >> � 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) >> � 5. Does xylene cause skin cancer? (Jenny Vega) >> � 6. RE: Does xylene cause skin cancer? (Settembre, Dana) >> � 7. Storage of Frozen Tissues (Dennis Ha
RE: [Histonet] Cleaning Bond Bulk Reagent Containers
Try after cleaning them rinse them with 100% alcohol and using a hair dryer! You really should purchase a spare set anyway, because sometime there are issues with the nozzles and the bond will not run without them. Your production would stop immediately and you would still have to purchase the bottles anyhow! Josie Britton HT Cheshire Medical Center Keene NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Thursday, February 23, 2012 11:19 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cleaning Bond Bulk Reagent Containers Hello Everyone! I am interested to find out how people are cleaning their Bond Bulk Reagent Containers. I don't need a procedure. I know how to do that. My question is how do you clean these containers when you use them day and night and they don't have an opportunity to be cleaned and completely dry? I'm looking for any feedback on how you do this. Purchasing a second set of bulk reagent bottles is not an option. Thanks in advance for your feedback. Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)266-2524 Histology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 99, Issue 30
Message 5: xylene Xylene is toxic and you should always wear gloves and a face mask when dealing with it. Dermal absorption is a lot slower than inhalation, but contact with organic solvents such as xylene is bad in general. With the issue of latex residue left on the slide, try using nitrile gloves. We use nitrile in our lab and we never have an issue with residue left on the slides. You may also want to wear two gloves on the hand that might come in contact with xylene because it can eat through gloves. I haven't found anything linking xylene as a human carcinogen, but it's toxic to the central nervous system as well as a known teratogen, so limiting any contact is advised. You can also use histoclear, another organic solvent that works really well that is far less toxic than xylene. On Feb 23, 2012, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to >histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at >histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Re: Histonet Digest, Vol 99, Issue 28 (Sheila Fonner) > 2. ADIPOPHILIN (Walls, Rachel) > 3. out of office (marilyn.a.we...@kp.org) > 4. RE: Does xylene cause skin cancer? (Mayer,Toysha N) > 5. Histology Lab Donations (Salim Yalcin Inan) > 6. Cleaning Bond Bulk Reagent Containers (Konop, Nicole) > > > -- > > Message: 1 > Date: Thu, 23 Feb 2012 06:44:25 -0500 > From: "Sheila Fonner" > Subject: RE: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > To: "'Madeleine Huey'" , > > Message-ID: <01ccf220$7e8ff2a0$7bafd7e0$@com> > Content-Type: text/plain;charset="iso-8859-1" > > ? You use regular superfrost plus slides for temperature verification of the > thermal pads? The slides to use are the superfrost plus slides from Ventana > that have a qc'd strip on them that will turn black if the temperatures of > pads are at acceptable levels. How do you prove that with a plain > superfrost plus slide? > > We use the suggested qc slides, but only do the test twice/yr instead of > quarterly. We re-wrote our procedure to indicate that this is what we do. > We then copy the slides onto paper and file for documentation purposes. You > could also keep the slides, if you have the room to file and store them. > CAP requires the written procedure as well as documentation that it has been > done. > > Sheila, HT (ASCP) > KDL Pathology > Knoxville, TN > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine > Huey > Sent: Wednesday, February 22, 2012 9:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 > > Message: 15 > Date: Wed, 22 Feb 2012 15:26:43 + > From: Amber McKenzie > Subject: [Histonet] Temp verifier slides - Ventana equipment > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <5a33c952bb67f4468af1f36d739212bc115ec...@jerry.gia.com> > Content-Type: text/plain; charset="us-ascii" > > Do y'all run the temp verifier slides for the quarterly maintenance for the > Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 > months, esp if you have multiple pieces of equipment. > > Amber, > > We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just > fine. > > Madeleine Huey BS, HTL (ASCP) QIHC > Supervisor - Pathology (IPOX & Histology) madelein...@elcaminohospital.org > > On Wed, Feb 22, 2012 at 10:01 AM, > wrote: >> Send Histonet mailing list submissions to >> � � � �histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> � � � �http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> � � � �histonet-requ...@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> � � � �histonet-ow...@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> � 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) >> � 2. RE: Processor Question (Davide Costanzo) >> � 3. Job Opening in Orange county California (Paula Lucas) >> � 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) >> � 5. Does xylene cause skin cancer? (Jenny Vega) >> � 6. RE: Does xylene cause skin cancer? (Settembre, Dana) >> � 7. Storage of Frozen Tissues (
[Histonet] Re: Does xylene cause skin cancer?
What's badly needed is a registry of death certificates of histotechnologists. The thing I'd want to examine would be the prevalence of myeloid leukemia and related diseases - known to be elevated in workers exposed to benzene. If such a correlation were found, it would mandate eliminating xylene from histology and cytology labs, and increasing precautions for handling resinous mounting media. The American Medical Association maintains such a registry of American physicians (or used to). It was used to establish that exposure to ionizing radiation (fluoroscopes and other X-ray equipment) was correlated with deaths from myeloid leukemia. Pathologists didn't have any particular problems - here I'd wonder about formaldehyde exposure and upper airway cancer. Pathologists get more exposure to formaldehyde than do histotechnologists. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cleaning Bond Bulk Reagent Containers
Hello Everyone! I am interested to find out how people are cleaning their Bond Bulk Reagent Containers. I don't need a procedure. I know how to do that. My question is how do you clean these containers when you use them day and night and they don't have an opportunity to be cleaned and completely dry? I'm looking for any feedback on how you do this. Purchasing a second set of bulk reagent bottles is not an option. Thanks in advance for your feedback. Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)266-2524 Histology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Lab Donations
Dear Histonet Members, I have been trying to set up a histology lab in our pharmacology department in Konya, Turkey. So far, I could only buy some basic stuff for cresyl violet staining in rat brain tissues. If you would like to get rid of some of your lab equipments and materials, or if you would like to donate some of them, I would like to accept any of your donations. Please contact me at: salim.i...@gmail.com Thank you very much in advance. Sincerely Yours, Salim -- Dr. Salim Yalcin Inan Assistant Professor Department of Pharmacology Meram Faculty of Medicine University of Konya 42080 Akyokus, Meram Konya, Turkey E-mail: salim.i...@gmail.com Phone: +90 530 967 9787 (Cellular) Phone: +90 332 223 6624 (Office-direct line) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Does xylene cause skin cancer?
We all have heard the reports that xylene causes cancer. It is a carcinogen. However only in cases where the user is extremely sensitive to xylene should you worry about a little bit getting on your skin every now and then. Don't bathe in it. Do not make it a habit. Wearing gloves (nitrile) and using other appropriate PPE should keep you safe. I wear gloves when I coverslip, change the machines and recycle. User safety is first, so check with the hospital to see what the safety department says. Change your gloves frequently when coverslipping. I have been a tech for 20 years and I am ok. I take the usual precautions with PPE and teach the same to my students. Toysha Mayer -- Message: 2 Date: Wed, 22 Feb 2012 13:48:11 -0500 From: Bob Richmond Subject: [Histonet] Re: Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I don't know of any evidence that xylene causes skin cancer. Concern is with absorption through the skin. The most likely problem is with the bone marrow - leukemia and related diseases - from aromatic hydrocarbons (xylene, toluene, benzene) - which of course are present in resinous mounting media even in "xylene free" laboratories. Latex gloves dissolve rapidly. Nitrile rubber is more resistant, though not very. I don't know about vinyl examination gloves. I don't wear gloves in this situation, but obviously a pathologist gets much less exposure than a histotechnologist does. I certainly wouldn't argue with anyone who wanted to wear them. Bob Richmond Samurai Pathologist Knoxville TN * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] out of office
I will be out of the office starting 02/23/2012 and will not return until 02/24/2012. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ADIPOPHILIN
Has anyone worked up the antibody, Adipophilin on the Ventana XT's? Thanks, Rachel rachel.wa...@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Histonet Digest, Vol 99, Issue 28
? You use regular superfrost plus slides for temperature verification of the thermal pads? The slides to use are the superfrost plus slides from Ventana that have a qc'd strip on them that will turn black if the temperatures of pads are at acceptable levels. How do you prove that with a plain superfrost plus slide? We use the suggested qc slides, but only do the test twice/yr instead of quarterly. We re-wrote our procedure to indicate that this is what we do. We then copy the slides onto paper and file for documentation purposes. You could also keep the slides, if you have the room to file and store them. CAP requires the written procedure as well as documentation that it has been done. Sheila, HT (ASCP) KDL Pathology Knoxville, TN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Wednesday, February 22, 2012 9:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28 Message: 15 Date: Wed, 22 Feb 2012 15:26:43 + From: Amber McKenzie Subject: [Histonet] Temp verifier slides - Ventana equipment To: "histonet@lists.utsouthwestern.edu" Message-ID: <5a33c952bb67f4468af1f36d739212bc115ec...@jerry.gia.com> Content-Type: text/plain; charset="us-ascii" Do y'all run the temp verifier slides for the quarterly maintenance for the Ventana XT and Ultra? Those slides are mighty expensive to buy every 3 months, esp if you have multiple pieces of equipment. Amber, We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just fine. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madelein...@elcaminohospital.org On Wed, Feb 22, 2012 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch) > 2. RE: Processor Question (Davide Costanzo) > 3. Job Opening in Orange county California (Paula Lucas) > 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks) > 5. Does xylene cause skin cancer? (Jenny Vega) > 6. RE: Does xylene cause skin cancer? (Settembre, Dana) > 7. Storage of Frozen Tissues (Dennis Hahn) > 8. Re: Does xylene cause skin cancer? (Rene J Buesa) > 9. RE: Does xylene cause skin cancer? (Debbie Faichney) > 10. Re: Storage of Frozen Tissues (Rene J Buesa) > 11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) > 12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam) > 13. RE: Does xylene cause skin cancer? (Hilary Smith) > 14. Histotech & Histology Supervisor Job in Naples, FL > (Melissa Phelan) > 15. Temp verifier slides - Ventana equipment (Amber McKenzie) > 16. RE: Does xylene cause skin cancer? (Goins, Tresa) > > > -- > > Message: 1 > Date: 21 Feb 2012 13:08:28 -0500 > From: "Darcy Bloch" > Subject: [Histonet] Seeking a Histology Supervisor in Connecticut > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > Slone Partners seeks a Histology Supervisor for our hospital > based > laboratory > > > The successful candidate redesign and be great at managing change. people in the department, including 2 supervisors. experience is a plus. > > > > Qualified certification, with high-volume laboratory. > > > > Special features of this position: have the opportunity to help > redesign this > > > If you meet these qualifications for this position, please submit your resume dar...@slonepartners.com. > > > > If you wish to be considered Tara Kochis at t...@slonepartners.com. > > > > All inquiries are kept confidential. > > > -- > > Message: 2 > Date: Tue, 21 Feb 2012 10:33:27 -0800 > From: Davide Costanzo > Subject: RE: [Histonet] Processor Question > To: "Gauch, Vicki" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <-2296715788321707800@unknownmsgid> > Content-Type: text/plain; charset=ISO-8859-1 > > Design flaw in the screen display. It is in the way of the chamber > when opening chamber. If your not careful you will break the screen. > Happens fairly often. > > Sent from my Windows Phone > From: Gauch, Vicki > Sent: 2/21/2012 9:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processor Question Hi everyone, We a
RE: [Histonet] Does xylene cause skin cancer?
But the gloves in that Ansell list are reusable, whereas I suppose that you mean the disposable Kimberley-Clark gloves? The thickness would be very different in that case. And I wouldn't be able to mount a slide with thick, re-use gloves. :) In any case, the doctor for my professional yearly medical exam always tells me to only use nitrile gloves in combination with xylene, and that xylene will get through after a few minutes. So I just avoid getting xylene on my hands altogether by using forceps to handle wet slides while coverslipping. --- Jonathan Cremer Laboratory Technician Gastro-enterologie KUL CDG Bus 811; Labo Experimentele Immunologie; Herestraat 49; 3000 Leuven; Belgium Van: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] namens Tony Henwood (SCHN) [tony.henw...@health.nsw.gov.au] Verzonden: donderdag 23 februari 2012 1:56 Aan: 'Hilary Smith'; Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Onderwerp: RE: [Histonet] Does xylene cause skin cancer? Wow, That's interesting; KIMBERLY-CLARK Nitrile Gloves are not resistant to xylene, whereas Ansell's Nitrile gloves are (see http://www.ansellpro.com/download/Ansell_7thEditionChemicalResistanceGuide.pdf) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilary Smith Sent: Thursday, 23 February 2012 1:52 AM To: Debbie Faichney; Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? You might want to go to something with even greater chemical resistance - thin nitrile is not recommended for use with xylene: http://www.kcproductselector.com/~/media/RelatedMedia/PDFs/Gloves/K2365_09_01_SN%20Chem%20Guide_v10.ashx According to our xylene MSDS: "The substance may be toxic to blood, kidneys, liver, mucous membranes, bone marrow, central nervous system (CNS). Repeated or prolonged exposure to the substance can produce target organs damage." I would definitely use gloves if I were you. Hilary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Faichney Sent: Wednesday, February 22, 2012 8:15 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] Does xylene cause skin cancer? If you can get a hold of them, try using Nitrile gloves as these have a higher chemical resistance than latex. I use them and change every 30 minutes to avoid breakthrough. Debbie Faichney Institute of Aquaculture University of Stirling UK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 22 February 2012 13:09 To: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] Does xylene cause skin cancer? There is no evidence in the literature about skin cancer produced by xylene, although dermatitis are well documented. Regardless you should use gloves whenever your hands can get in contact with any chemical as a good safety practice. If your colleagues do not want to use gloves, that is their prerogative, as is yours to wear them. René J. --- On Tue, 2/21/12, Jenny Vega wrote: From: Jenny Vega Subject: [Histonet] Does xylene cause skin cancer? To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 21, 2012, 10:17 PM I am asking this because in my job we mount slides by hand, and my coworkers don't like to use gloves because it leaves a residue of latex in the back of the slides. I really don't feel comfortable mounting without gloves because I heard that xyelene can cause cancer. Some people I know personally has told me that this is not possible, but I read in some places that xylene could a possible carcinogen. I have already gotten contact with xylene in my hands a couple of times and I am worried. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __
