[Histonet] (no subject)
Greetings, friend! http://nahku3m.zymichost.com/party.php?cytjs=59&hosyzapi=165&ilaze=56 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Please unsubscribe. Damaris E. Beil "Live Well, Laugh Often, Love Much" ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: Re: [Histonet] Antibody diluent
--- On Sun, 3/11/12, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Antibody diluent To: "Dr. Mohammad Golam Mostafa" Date: Sunday, March 11, 2012, 11:13 AM Yes, there will be a problem, namely that the antibodies (proteins) need a diluted protein base to "float", and a preservative to prevent bacterial growth. Under separate cover I am sending you my recipe to prepare the antibody diluent "in house". René J. --- On Sat, 3/10/12, Dr. Mohammad Golam Mostafa wrote: From: Dr. Mohammad Golam Mostafa Subject: [Histonet] Antibody diluent To: rjbu...@yahoo.com Date: Saturday, March 10, 2012, 1:20 PM I am a Pathologist of Bangladesh. Is there any problem, if I use TBS as antibody diluents? Please let me know. Dr. Mohammad Golam Mostafa Dhaka, Bangladesh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] false positive CD31?
Dear histonet! Has anyone seen granular, fine dot-like, cytoplasmic CD-31 staining in tumorcells, that was thought to be false positive? It looks like really specific staining, but my pathologist sent this case to an expert, who couldn't reproduce this staining. The expert is really an expert and his diagnosis is not to be bothered. We stain with dako-ab on benchmark Ultra (CC1 mild, 32min plus amplifier, 1:50). thank you Gudrun Lang Biomed. Analytikerin histolab, Linz, Austria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thick Sections cut at 8 microns and up
Bharti One thing that usually works is to have a thin layer of a softer melting point wax on the top and bottom of the block, this should allow the sections to adhere to each other and not roll up. Need only a 5 degree or so difference in the wax for this to work. Another is to use a slightly lower melting point wax for your embedding. Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bharti Parihar [bhartolog...@gmail.com] Sent: Saturday, March 10, 2012 10:03 PM To: Histonet Archive Subject: [Histonet] Thick Sections cut at 8 microns and up Hello Hitsonetters! I worked on a lab today for my Histology program which called for cutting brain sections at 12 microns. The lab we are doing is Weils to demonstrate myelin. Man oh man was it hard to cut 12 micron sections. Apparently 10 microns work for this stain as well which I managed to do that more easily than 12 but I used the idea of using a small brush to help guide the section down the knife plate like when cutting frozen sections so it wouldn't instantly roll up into a tube which is what the 12 micron sections kept doing. Any tricks to cutting thick sections? Suggestions for the student here? Thanks. -Bharti Parihar ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
