[Histonet] (no subject)

[Khaire Dai]

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[Histonet] Leica Bond IHC Platform

[Wellen, Terrence D. :LPH Lab]

Does anyone have any experience with this product?


Terrence Wellen HT(ASCP)
Legacy Good Samaritan Hospital
Portland, OR

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[Histonet] Region III final reminder

[Shirley A. Powell]

Hi Histotechs,

This email is to remind you of the Georgia Society for Histotechnology hosting 
Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,.  This 
is next week friends.  We will NOT be in the conference center as last year, 
and that is a GOOD thing.  We will be in the ballroom area where we will have 
more space.  We have far exceeded our original block of rooms, extended the 
deadline and now if the Mountain Creek Inn has any available, they will allow 
the GSH room rate.  Please use the GSH Group # # 78K711 to get the discounted 
room rate of $109.  Make reservations now at 1-800-225-5292.

If you have not registered for the meeting please do so.PLEASE REGISTER NOW 
AS "AWAITING FUNDS"  if you are waiting approval for the meeting go ahead and 
register for your workshops, luncheon, dinner and note on the form that you are 
awaiting funds  below the total line.  When approved, then send the funds, or 
if time does not allow, pay on site.  But please register as soon as possible, 
don't wait, workshops are filling up fast.

  We also will have Histotalk's Dave Kemler at the meeting to interview 
some of our attendees as well.

  Also make plans to attend the Carriage and Horses Dinner on Saturday 
night for great food, great friends and good entertainment.  The deadline for 
reservations for the dinner is April 9th, that is next Monday.  Please make 
sure you fill out your registration form completely and email the form to Anne 
Taylor, GSH Treasurer to confirm your workshops as awaiting funds and then pay 
at the registration desk on site since time is short.

  If you have questions or concerns please contact Mike Ayers at 
lmay...@charter.net,  Wanda Simons at 
wandr...@att.net or myself at 
powell...@mercer.edu.

 We are excited to have 29 vendors exhibiting at our meeting and more 
signing up as we approach the meeting.

BioCare  ~Sponsor of  new  IHC Award

BioGenex

B/R Instruments

Cancer Diagnostics

Cell Marque

Choice Medical

Clarient~ sponsoring dinner at Dagher's

Dako

EMS

Epitomics

General Data

IMEB

Lab Storage

Leica Microsystems  ~ sponsoring dinner at Dagher's

Leica Biosystems

Mopec

PolyScientific R&D - sponsor of new award "TBA"

Sakura - Sponsor of GSH Histotechnologist of the Year Award

Stat Lab

Southeast Pathology

Thermo Fisher -

TBS

ScyTek

StatLab

Ventana



Those Not attending but supporting GSH are:

Anatech - Unmanned table

CL Sturkey - Door prizes

LABSCO - Sponsoring a break

Newcomer - Sponsoring a Break and provided totes

Come Experience 
 Histotechnology - Southern Style



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[Histonet] Bone (Trichrome)

[Lawrence Allen]

Why not sent in 10 % formalin. Freezing it may change the morphology of the 
bone to fixation after thawing. I would give that a try. After all, you are 
running 3 different stains at one time in order.

Lawrence S Allen
Lead Histotechnologist
Dorm VA Medical Center
Columbia, SC


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Re: [Histonet] EM tissue processors Q's

[Jan Shivers]

We use the Leica TP for our samples and endorse it.

Jan Shivers
IHC/Histo/EM Section Head
Veterinary Diagnostic Lab
UMN College of Veterinary Medicine
St. Paul, MN

On Wed, Apr 4, 2012 at 2:26 PM, Morken, Timothy <
timothy.mor...@ucsfmedctr.org> wrote:

> Hi, we're looking around at different automated TEM tissue processors. We
> have an RMC 5160 but it has had a lot of problems so we are considering
> something else. I've looked online at Leica TP and Lynx II for routine
> processing. We may get to MW processing later, but the price is higher than
> authorized right now.
>
> Any suggestions or comments?
>
> Thanks for any info!
>
>
> Tim Morken
> Supervisor, Electron Microscopy
> Department of Pathology
> UC San Francisco Medical Center
> 505 Parnassus Ave, Box 1656
> Room S570
> San Francisco, CA 94115
>
> (415) 353-2673 (ph)
> (415) 514-3403 (fax)
> tim.mor...@ucsfmedctr.org
>
>
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[Histonet] Specimen Identity Procedure

[Scott, Allison D]

Hello again to all in histoland.  Does anyone have a procedure for describing 
the system for maintaining the identity of every specimen through processing 
and block and slide preparation.  This is on the CAP checklist (ANP.21050).  
Any help will be greatly appreciated.


Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
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[Histonet] EM tissue processors Q's

[Morken, Timothy]

Hi, we're looking around at different automated TEM tissue processors. We have 
an RMC 5160 but it has had a lot of problems so we are considering something 
else. I've looked online at Leica TP and Lynx II for routine processing. We may 
get to MW processing later, but the price is higher than authorized right now.

Any suggestions or comments?

Thanks for any info!


Tim Morken
Supervisor, Electron Microscopy
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94115

(415) 353-2673 (ph)
(415) 514-3403 (fax)
tim.mor...@ucsfmedctr.org


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[Histonet] Grossing rules

[Amber McKenzie]

Are there any guidelines on how to do GI grossing?  At what size to you bisect? 
 Do you call 5 or more pieces multiple or do you count all the pieces?  How 
many categories are there: 1, 2, 3, several, multiple, etc...At what size is it 
considered a fragment and what size is a polyp?  Thanks!

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[Histonet] Automatic knife sharpener

[Maxim Peshkov]

Dear Histonetters!
Can anybody to advise, what type automatic knife sharpener
we can buy in Europe (Germany) instead Leica SP9000,
which now is unavailable for sale? What vendors?
We do not like a Shandon "Autosharp 5" type sharpener.
 Maxim Peshkov
 Russia,
 Taganrog.
 mailto:maxim...@mail.ru


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[Histonet] Re: Massons Trichrome on decalcified bone

[gayle callis]

Dear Sara,  

 

You wrote: 

I work at an Orthopaedic research lab and I have been having some trouble
getting our trichromes to work on our bone. I have tried a masson's, gomori,
and goldner's and in all cases the bone stained red.

 

Our bone is arrive to our lab frozen, we fix them in formalin, embed in
paraffin, and decal with EDTA. I also post fix with Bouins before staining.

 

Techs that worked in this lab before me were also getting the same red
staining of bone.

 

Is there something during processing that could cause this reaction? We fix
in formalin, dehydrate through graded alcohols, and clear in xylene.

 




I doubt your processing has any effect on Mass Tri staining.   However,
incomplete decalcification can but I suspect it may be the staining protocol
itself.   Using a simple weight loss/weight gain decalcification end point
test with EDTA is a good idea, and can be used for acid decalcification
endpoint testing IF you are not presently using an endpoint test. 

 

Years ago, I visited the AFIP bone lab, and acquired a Massons Trichrome
protocol which worked much better than the standard Massons Trichrome found
in most textbooks.  It never failed to work well with our decalcified bone
work although we did use acid decalcification.This is NOT a kit method.
All reagents are made up in house, and post mordant heating Bouins is NOT
done in a microwave.  We preferred to let the sections sit in Boiuns
overnight  at RT, or heat in a water bath.   We never used a dry heat,
incubator type oven where one gets uneven heating in the chamber.  

 

There is more to doing Massons Trichrome on decalcified bone other than just
following the recipe from a textbook. One of the best discussions on
understanding the chemistry/theory of trichrome staining is found in Sheehan
and Hrapchak, Theory and Practice of Histotechnology.

 

The AFIP method has some different staining times, plus how to remove the
biebrich scarlet/acid fuchsin from collagen BEFORE going into aniline blue,
a much more controlled staining method.It also has a modified Weigerts
Iron hematoxylin that is superior, a bit more concentrated since Iron
hematoxylin tends to be removed by the acidic staining solutions.   I will
be happy to send these methods to you privately.  

 

Gayle M. Callis 

HTL/HT/MT(ASCP)  

 

 

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[Histonet] Glassware washing

[Oneil, Beth Ann]

Our laboratory does about half of it's daily special stains manually which 
means we go through a lot of Copeland jars.  We currently wash everything by 
hand using liquinox with a splash of bleach in the water.  We rinse with tap 
water.  My question is how do other labs address the CAP issue of testing their 
glassware for detergent removal.  When I worked in the chemistry lab, we didn't 
go through a lot of glassware so we were able to take a monthly bin of dirty 
glassware to sterile processing for cleaning, and then just check one 
representative piece of glassware from the batch for detergent.  I can't figure 
out how my lab can check our glassware when there is so much, we wash it all 
throughout the day.  Thank you for any help.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor/Technical Specialist
West Virginia University Hospitals
304-293-7629 (office)
304-293-6014 (lab)




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[Histonet] New Position Alert - IHC Field Tech Atlanta

[Matt Ward]

Good Afternoon Histonet,



We have had a global leader in IHC and Histology open a field based IHC
support opportunity covering the Southeast Region. The ideal location would
be to be based in Atlanta and the position is open due to promotion.



If you or anyone you may know has a strong background in IHC and is looking
to break out of the lab into a field role please contact me directly to
learn more.



The position offers a Base Salary + Bonus + Full Benefits (Car Allowance,
Corporate Credit Card, Cell Phone, Laptop, Home Office, Full Health, 401k).



Regards,





Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com
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RE: [Histonet] Slippery Floor due to paraffin

[Sebree Linda A]

Peel off film here...works well. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, April 04, 2012 11:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slippery Floor due to paraffin

Hello to all in histoland.  What are histology labs doing to combat the
slipperiness of the floor due to paraffin.  Are you using rugs, peel
away films ?  Any help would be greatly appreciated.


Allison  Scott  HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026

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If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
your computer system.

To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance
Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR
Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is
confidential and/or privileged.  This e-mail may also be confidential
and/or privileged under Texas law.  The e-mail is for the use of only
the individual or entity named above.  If you are not the intended
recipient, or any authorized representative of the intended recipient,
you are hereby notified that any review, dissemination or copying of
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[Histonet] Slippery Floor due to paraffin

[Scott, Allison D]

Hello to all in histoland.  What are histology labs doing to combat the 
slipperiness of the floor due to paraffin.  Are you using rugs, peel away films 
?  Any help would be greatly appreciated.


Allison  Scott  HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

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AW: [Histonet] Masson's Trichrome for Bone

[Gudrun Lang]

Perhaps I am wrong.
But doesn't the trichrome differentiate between mature and fresh bone
tissue? Old one - red; new one - blue (or green) Depending on the amount of
collagen.
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sara
Landschoot
Gesendet: Dienstag, 03. April 2012 21:34
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Masson's Trichrome for Bone

Hi!

I work at an Orthopaedic research lab and I have been having some trouble
getting our trichromes to work on our bone. I have tried a masson's, gomori,
and goldner's and in all cases the bone stained red.

Our bone is arrive to our lab frozen, we fix them in formalin, embed in
paraffin, and decal with EDTA. I also post fix with Bouins before staining.

Techs that worked in this lab before me were also getting the same red
staining of bone.

Is there something during processing that could cause this reaction? We fix
in formalin, dehydrate through graded alcohols, and clear in xylene.

Thanks,
Sara
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