Re: [Histonet] Loss of Matrigel material during staining

[Carrie Schray]

My experience with this is that the processing schedule is too long
and probably too much heat. I had some skin that was grown in a well
plate from human cells processed on a biopsy run, they cut fantastic
then the gel rolled onto itself during staining. Once we reduced the
processing schedule and took heat away on the processing reagents it
was much better.
And, try to use super sticky slides, such as those you would use with
bone samples.

Carrie L. Schray
Univ. of Michigan Medical School
Unit for Laboratory Animal Medicine
Ann Arbor, Michigan


On 4/5/12, Amy Porter  wrote:
> To all:  I have a client that we are attempting to stain H & E / as well as
> immuno on FFPE mouse skin with matrigel plugs.  These things section like a
> dream and then when we attempt to stain only the matrigel part of the sample
> is falling off.  Does anyone have any tricks with this stuff they would be
> willing to share?  We are using charged slides and cutting at 5-6 microns
> per the client.  Thanks -
>
>
>
> Amy S. Porter, HT(ASCP) QIHC
>
> Michigan State University
>
> Investigative HistoPathology Laboratory
>
> William S. Spielman, Ph.D. - Director
>
> Patricia K. Senagore, M.D. - Consulting Pathologist
>
> Department of Physiology / Human Pathology
>
> Biomedical Physical Sciences Building
>
> 567 Wilson Road - Room 2133
>
> East Lansing, MI  48824-3320
>
> Phone:  517-884-5026
>
> Fax:  517-432-1368
>
> port...@msu.edu
>
> www.humanpathology.msu.edu
>
>
>
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[Histonet] Microwave use in histo lab

[Hart, Heather]

Hello everyone!

I am currently taking a class titled Current Trends & Applications in Applied 
Science & Technology which is essentially a capstone course for my BSAST degree 
completion.   This course requires a four part research paper on any technology 
of choice in the (students) related field.  The topic I have chosen is 
Microwave Use in the Histology Lab.  I am trying to gather information for the 
third module in which I need to address the topics listed below.  I would 
appreciate any personal input or opinions about the topic per guidelines listed.

Thank you for your help!

Heather Hart, MLT (ASCP)


Various Perspectives and Opinions
   Provide alternative perspectives from experts on technology
   Political implications and influences
   Public opinion on technology such as the media, consumers and community
   Your assessment of how effective the initial planning and risk 
assessment was to the implementation and usage of the technology

   “You are encouraged to access other users and/or associated 
technologists in order to gain insight into various perspectives and fully 
understand the applications of the technology selected.”

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Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Kim Donadio]

My lab can pass any inspection I have no fear 
Bring it on
utube.com/index?desktop_uri=%2F&gl=US#/watch?v=gAQCbczCt8s

Sent from my iPhone

On Apr 5, 2012, at 7:00 PM, Katelin Lester  wrote:

> We also received this notice. We contacted our local CLIA office who had
> heard of it this week as well. We are a small lab, so we are not sure yet
> how this change will impact us. I'd also be curious to know what smaller,
> private labs are planning on doing.
> -- 
> Katelin Lester, HTL
> Gastroenterology Specialists of Oregon, P.C.
> Pathology Laboratory
> (971) 224-2408
> 
> On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence  wrote:
> 
>> We have received notification from AETNA that they now require non-hospital
>> labs to be accredited by CLIA and CAP.  The letter makes it obvious that by
>> making such a request that they are not aware that CLIA assigned deemed
>> status to CAP and CLIA is actually the gatekeeper.  Secondly we are told to
>> be registered by May 1st and accredited by August 1st (which CAP says is
>> impossible) or we will have to send our lab to either Quest or Ameripath
>> which includes Dermpath Diagnostics division.  It fails to mention that
>> there are other CAP accredited non hospital labs in our state.  The Aetna
>> contact number is either 'mailbox full" or even after leaving a message, no
>> return call.  Me thinks me smells a rat.
>> 
>> 
>> 
>> If you are a non-hospital lab, have you heard of this?  Does your
>> dematopathologist or pathologist know this is coming?  I am interested in
>> your comments.
>> 
>> 
>> 
>> Carol M. Torrence, HT(ASCP)CM
>> 
>> 
>> 
>> 
>> 
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Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Katelin Lester]

We also received this notice. We contacted our local CLIA office who had
heard of it this week as well. We are a small lab, so we are not sure yet
how this change will impact us. I'd also be curious to know what smaller,
private labs are planning on doing.
-- 
Katelin Lester, HTL
Gastroenterology Specialists of Oregon, P.C.
Pathology Laboratory
(971) 224-2408

On Thu, Apr 5, 2012 at 12:16 PM, Carol Torrence  wrote:

> We have received notification from AETNA that they now require non-hospital
> labs to be accredited by CLIA and CAP.  The letter makes it obvious that by
> making such a request that they are not aware that CLIA assigned deemed
> status to CAP and CLIA is actually the gatekeeper.  Secondly we are told to
> be registered by May 1st and accredited by August 1st (which CAP says is
> impossible) or we will have to send our lab to either Quest or Ameripath
> which includes Dermpath Diagnostics division.  It fails to mention that
> there are other CAP accredited non hospital labs in our state.  The Aetna
> contact number is either 'mailbox full" or even after leaving a message, no
> return call.  Me thinks me smells a rat.
>
>
>
> If you are a non-hospital lab, have you heard of this?  Does your
> dematopathologist or pathologist know this is coming?  I am interested in
> your comments.
>
>
>
> Carol M. Torrence, HT(ASCP)CM
>
>
>
>
>
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Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Jesus Ellin]

I think this is just the beginning,, hold on to your pants

Sent from my iPad

On Apr 5, 2012, at 2:16 PM, "Kim Donadio"  wrote:

> 
> http://www.mohscollege.org/president/AETNAletter.pdf
> 
> Sent from my iPhone
> 
> On Apr 5, 2012, at 5:09 PM, Kim Donadio  wrote:
> 
>> Hmm I found a letter regarding this.
>> 
>> mohscollege.org/president/AETNAletter
>> 
>> I must say the time restraint  seems short but I am not surprised they are 
>> wanting it. With today's reimbursement rates and the economy we are in ins 
>> companies want to insure they get the highest quality of service for their  
>> dollars. 
>> I'm a little surprised they specifically want CAP though but nit that much. 
>> CLIA has deemed CAP authority to guide in the area of quality accreditation. 
>>  
>> No need to panic though. Remember. Times are changing and prosperity favors 
>> the ones who act upon knowledge :) 
>> Kim D
>> Sent from my iPhone. 
>> 
>> On Apr 5, 2012, at 3:16 PM, "Carol Torrence"  wrote:
>> 
>>> We have received notification from AETNA that they now require non-hospital
>>> labs to be accredited by CLIA and CAP.  The letter makes it obvious that by
>>> making such a request that they are not aware that CLIA assigned deemed
>>> status to CAP and CLIA is actually the gatekeeper.  Secondly we are told to
>>> be registered by May 1st and accredited by August 1st (which CAP says is
>>> impossible) or we will have to send our lab to either Quest or Ameripath
>>> which includes Dermpath Diagnostics division.  It fails to mention that
>>> there are other CAP accredited non hospital labs in our state.  The Aetna
>>> contact number is either 'mailbox full" or even after leaving a message, no
>>> return call.  Me thinks me smells a rat.
>>> 
>>> 
>>> 
>>> If you are a non-hospital lab, have you heard of this?  Does your
>>> dematopathologist or pathologist know this is coming?  I am interested in
>>> your comments.
>>> 
>>> 
>>> 
>>> Carol M. Torrence, HT(ASCP)CM 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> ___
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> ___
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Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Kim Donadio]

http://www.mohscollege.org/president/AETNAletter.pdf

Sent from my iPhone

On Apr 5, 2012, at 5:09 PM, Kim Donadio  wrote:

> Hmm I found a letter regarding this.
> 
> mohscollege.org/president/AETNAletter
> 
> I must say the time restraint  seems short but I am not surprised they are 
> wanting it. With today's reimbursement rates and the economy we are in ins 
> companies want to insure they get the highest quality of service for their  
> dollars. 
> I'm a little surprised they specifically want CAP though but nit that much. 
> CLIA has deemed CAP authority to guide in the area of quality accreditation.  
> No need to panic though. Remember. Times are changing and prosperity favors 
> the ones who act upon knowledge :) 
> Kim D
> Sent from my iPhone. 
> 
> On Apr 5, 2012, at 3:16 PM, "Carol Torrence"  wrote:
> 
>> We have received notification from AETNA that they now require non-hospital
>> labs to be accredited by CLIA and CAP.  The letter makes it obvious that by
>> making such a request that they are not aware that CLIA assigned deemed
>> status to CAP and CLIA is actually the gatekeeper.  Secondly we are told to
>> be registered by May 1st and accredited by August 1st (which CAP says is
>> impossible) or we will have to send our lab to either Quest or Ameripath
>> which includes Dermpath Diagnostics division.  It fails to mention that
>> there are other CAP accredited non hospital labs in our state.  The Aetna
>> contact number is either 'mailbox full" or even after leaving a message, no
>> return call.  Me thinks me smells a rat.
>> 
>> 
>> 
>> If you are a non-hospital lab, have you heard of this?  Does your
>> dematopathologist or pathologist know this is coming?  I am interested in
>> your comments.
>> 
>> 
>> 
>> Carol M. Torrence, HT(ASCP)CM 
>> 
>> 
>> 
>> 
>> 
>> ___
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>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Kim Donadio]

Hmm I found a letter regarding this.

mohscollege.org/president/AETNAletter

I must say the time restraint  seems short but I am not surprised they are 
wanting it. With today's reimbursement rates and the economy we are in ins 
companies want to insure they get the highest quality of service for their  
dollars. 
I'm a little surprised they specifically want CAP though but nit that much. 
CLIA has deemed CAP authority to guide in the area of quality accreditation.  
No need to panic though. Remember. Times are changing and prosperity favors the 
ones who act upon knowledge :) 
Kim D
Sent from my iPhone. 

On Apr 5, 2012, at 3:16 PM, "Carol Torrence"  wrote:

> We have received notification from AETNA that they now require non-hospital
> labs to be accredited by CLIA and CAP.  The letter makes it obvious that by
> making such a request that they are not aware that CLIA assigned deemed
> status to CAP and CLIA is actually the gatekeeper.  Secondly we are told to
> be registered by May 1st and accredited by August 1st (which CAP says is
> impossible) or we will have to send our lab to either Quest or Ameripath
> which includes Dermpath Diagnostics division.  It fails to mention that
> there are other CAP accredited non hospital labs in our state.  The Aetna
> contact number is either 'mailbox full" or even after leaving a message, no
> return call.  Me thinks me smells a rat.
> 
> 
> 
> If you are a non-hospital lab, have you heard of this?  Does your
> dematopathologist or pathologist know this is coming?  I am interested in
> your comments.
> 
> 
> 
> Carol M. Torrence, HT(ASCP)CM 
> 
> 
> 
> 
> 
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[Histonet] 5. Automatic knife sharpener, Histonet Digest, Vol 101, Issue 4

[Keith]

Maxim, I have used and would recommend the Hacker H/I-76 Microtome Knife 
Sharpener.  With this sharpener, honing and stropping a microtome knife takes 
only a few minutes, and results in a better knife edge than the automatic glass 
plate honing/stropping method.  Also, as long as the knife edge has not been 
nicked or damaged, the microtome knife need only be stropped as needed.    I 
like you choice to stay away from the disposable knife blades.  I preferred 
using the solid knife to the disposable blades because of the strength and 
stability at the cutting edge (less of a chance for the chatter artifact).  The 
very sharp, slightly biconcave edge on the blade (see profile B on their 
website, below) allows 4 micron sections to be easily sectioned from the 
paraffin block.  A new H/I-76 can be ordered directly from Hacker,  
http://www.hackerinstruments.com/tissueprep.htm.   For less of an investment... 
be wary, there are a few available on the secondary market.
 
Keith Mikoff, HTL/HT(ASCP)
Sacramento, California
mik...@gmail.com

Message: 5
Date: Wed, 4 Apr 2012 23:20:15 +0300
From: Maxim Peshkov 
Subject: [Histonet] Automatic knife sharpener
To: histonet@lists.utsouthwestern.edu
Message-ID: <1182039392.20120404232...@mail.ru>
Content-Type: text/plain; charset=windows-1251

Dear Histonetters!
Can anybody to advise, what type automatic knife sharpener
we can buy in Europe (Germany) instead Leica SP9000,
which now is unavailable for sale? What vendors?
We do not like a Shandon "Autosharp 5" type sharpener.
Maxim Peshkov
Russia,
Taganrog.
mailto:maxim...@mail.ru
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RE: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Troyer, Dean A.]

Was the notification in the form of a letter (snail mail) or another form such 
as email?
 
Dean Troyer



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Carol Torrence
Sent: Thu 4/5/2012 3:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital labs



We have received notification from AETNA that they now require non-hospital
labs to be accredited by CLIA and CAP.  The letter makes it obvious that by
making such a request that they are not aware that CLIA assigned deemed
status to CAP and CLIA is actually the gatekeeper.  Secondly we are told to
be registered by May 1st and accredited by August 1st (which CAP says is
impossible) or we will have to send our lab to either Quest or Ameripath
which includes Dermpath Diagnostics division.  It fails to mention that
there are other CAP accredited non hospital labs in our state.  The Aetna
contact number is either 'mailbox full" or even after leaving a message, no
return call.  Me thinks me smells a rat.



If you are a non-hospital lab, have you heard of this?  Does your
dematopathologist or pathologist know this is coming?  I am interested in
your comments.



Carol M. Torrence, HT(ASCP)CM





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RE: [Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Pratt, Caroline]

Actually, I called the contact person on the letter and received a call
back within 24 hours.  I asked her if TJC and COLA would be acceptable
in lieu of CAP and she is going to get an answer to me by next week.  I
am hoping the goal of this is simply to ensure and support high quality
patient care, but I agree that the letter should not read as an
advertisement for "preferred laboratories".



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol
Torrence
Sent: Thursday, April 05, 2012 3:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Aetna requiring CAP accreditation for non-hospital
labs

We have received notification from AETNA that they now require
non-hospital
labs to be accredited by CLIA and CAP.  The letter makes it obvious that
by
making such a request that they are not aware that CLIA assigned deemed
status to CAP and CLIA is actually the gatekeeper.  Secondly we are told
to
be registered by May 1st and accredited by August 1st (which CAP says is
impossible) or we will have to send our lab to either Quest or Ameripath
which includes Dermpath Diagnostics division.  It fails to mention that
there are other CAP accredited non hospital labs in our state.  The
Aetna
contact number is either 'mailbox full" or even after leaving a message,
no
return call.  Me thinks me smells a rat.

 

If you are a non-hospital lab, have you heard of this?  Does your
dematopathologist or pathologist know this is coming?  I am interested
in
your comments.

 

Carol M. Torrence, HT(ASCP)CM 

 

 

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[Histonet] Loss of Matrigel material during staining

[Amy Porter]

To all:  I have a client that we are attempting to stain H & E / as well as
immuno on FFPE mouse skin with matrigel plugs.  These things section like a
dream and then when we attempt to stain only the matrigel part of the sample
is falling off.  Does anyone have any tricks with this stuff they would be
willing to share?  We are using charged slides and cutting at 5-6 microns
per the client.  Thanks -

 

Amy S. Porter, HT(ASCP) QIHC

Michigan State University

Investigative HistoPathology Laboratory

William S. Spielman, Ph.D. - Director

Patricia K. Senagore, M.D. - Consulting Pathologist

Department of Physiology / Human Pathology

Biomedical Physical Sciences Building 

567 Wilson Road - Room 2133

East Lansing, MI  48824-3320

Phone:  517-884-5026

Fax:  517-432-1368

port...@msu.edu

www.humanpathology.msu.edu

 

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[Histonet] Aetna requiring CAP accreditation for non-hospital labs

[Carol Torrence]

We have received notification from AETNA that they now require non-hospital
labs to be accredited by CLIA and CAP.  The letter makes it obvious that by
making such a request that they are not aware that CLIA assigned deemed
status to CAP and CLIA is actually the gatekeeper.  Secondly we are told to
be registered by May 1st and accredited by August 1st (which CAP says is
impossible) or we will have to send our lab to either Quest or Ameripath
which includes Dermpath Diagnostics division.  It fails to mention that
there are other CAP accredited non hospital labs in our state.  The Aetna
contact number is either 'mailbox full" or even after leaving a message, no
return call.  Me thinks me smells a rat.

 

If you are a non-hospital lab, have you heard of this?  Does your
dematopathologist or pathologist know this is coming?  I am interested in
your comments.

 

Carol M. Torrence, HT(ASCP)CM 

 

 

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[Histonet] Histology Openings for full and part time

[Brannon Owens]

Allied Search Partners currently has openings for the following (full job
descriptions available):

Mohs Histotech- Denver, CO area- full time

Histotechnician or Histotechnologist ­ Portland, OR area- part time

Interested candidates should submit their updated resumes to
bran...@alliedsearchpartners.com.  No more information will be given about
location of the lab at this time.  Thank you.
-- 
*If you wish to no longer receive emails from Allied Search Partners please
respond to this email message with "remove."
 
Brannon Owens, Recruitment Manager
LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823

Allied Search Partners

T: 888.388.7571 ext. 106

F: 888.388.7572

www.alliedsearchpartners.com 

Tell us about your experience with ASP by clicking on this link:
http://ratepoint.com/tellus/82388  

 

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[Histonet] Re: Grossing rules

[Bob Richmond]

Amber McKenzie asks: >>Are there any guidelines on how to do GI
grossing? At what size to you bisect? Do you call 5 or more pieces
multiple or do you count all the pieces? How many categories are
there: 1, 2, 3, several, multiple, etc.? At what size is it considered
a fragment and what size is a polyp?<<

This is very poorly standardized, and basically I can tell you my own
practice. I bisect polyps 4 mm in size or greater - that's about as
steady as my hands are. I count all the pieces up to about 10. I don't
understand the question about categories. It's a polyp if it looks
like a polyp.

I don't measure specimens of ordinary size, since I cannot do it
accurately. I'll sometimes refer to "a single quite small specimen."

Examples of dictation:
1234. Received in fixative labeled "John Doe" and "duodenum" is a
single biopsy specimen submitted in cassette A.
2345. Received in fixative labeled "Mary Doe" and "esophagus" are four
biopsy specimens submitted together in cassette A.
3456. Received in fixative labeled "John Roe" and "transverse colon"
is a single 5 mm dull red polyp with a very short stalk, divided
longitudinally into two pieces, and entirely submitted in cassette A.

As I said, that's just what I do when I gross for myself.

Bob Richmond
Samurai Pathologist
Knoxville TN
Received in fixative is a single biopsy specimen submitted in cassette A.

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[Histonet] Re:SAVE MAsson's in BONE info

[Vicki Kalscheur]

- Original Message - 
From: 

To: 
Sent: Thursday, April 05, 2012 12:05 PM
Subject: Histonet Digest, Vol 101, Issue 4



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Today's Topics:

  1. RE: Slippery Floor due to paraffin (Sebree Linda A)
  2. New Position Alert - IHC Field Tech Atlanta (Matt Ward)
  3. Glassware washing (Oneil, Beth Ann)
  4. Re:  Massons Trichrome on decalcified bone (gayle callis)
  5. Automatic knife sharpener (Maxim Peshkov)
  6. Grossing rules (Amber McKenzie)
  7. EM tissue processors Q's (Morken, Timothy)
  8. Specimen Identity Procedure (Scott, Allison D)
  9. Re: EM tissue processors Q's (Jan Shivers)
 10. Bone (Trichrome) (Lawrence Allen)
 11. Region III final reminder (Shirley A. Powell)
 12. Leica Bond IHC Platform (Wellen, Terrence D. :LPH Lab)
 13. (no subject) (Khaire Dai)
 14. please don't sent e-mail (mervata...@aol.com)
 15. Re: Massons Trichrome on decalcified bone (Sara Landschoot)
 16. RE: Leica Bond IHC Platform (Sue Hunter)
 17. Re: Re: Massons Trichrome on decalcified bone (Jack Ratliff)
 18. RE: Leica Bond IHC Platform (Rathborne, Toni)
 19. RE: Leica Bond IHC Platform (Sue Hunter)
 20. Commercial clearing and bluing solutions in Europe
 (Jackie O'Connor)
 21. JOB OPENING (Jeffrey Howery)


--

Message: 1
Date: Wed, 4 Apr 2012 12:21:24 -0500
From: "Sebree Linda A" 
Subject: RE: [Histonet] Slippery Floor due to paraffin
To: "Scott, Allison D" ,

Message-ID:


Content-Type: text/plain; charset="US-ASCII"

Peel off film here...works well.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, April 04, 2012 11:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slippery Floor due to paraffin

Hello to all in histoland.  What are histology labs doing to combat the
slipperiness of the floor due to paraffin.  Are you using rugs, peel
away films ?  Any help would be greatly appreciated.


Allison  Scott  HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026

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Message: 2
Date: Wed, 4 Apr 2012 13:31:10 -0400
From: Matt Ward 
Subject: [Histonet] New Position Alert - IHC Field Tech Atlanta
To: histonet@lists.utsouthwestern.edu
Message-ID: <33b696097ef326f96a51a0f52df63...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Good Afternoon Histonet,



We have had a global leader in IHC and Histology open a field based IHC
support opportunity covering the Southeast Region. The ideal location
would
be to be based in Atlanta and the position is open due to promotion.



If you or anyone you may know has a strong background in IHC and is
looking
to break out of the lab into a field role please contact me directly to
learn more.



The position offers a Base Salary + Bonus + Full Benefits (Car Allowance,
Corporate Credit Card, Cell Phone, Laptop, Home Office, Full Health,
401k).



Regards,





Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

www.personifysearch.com


--

Message: 3
Date: Wed, 4 Apr 2012 18:08:14 +
From: "Oneil, Beth Ann" 
Subject: [Histonet] Glassware washing
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<3ceb8ebcf9c7a648b9694b5696462a71e...@nt-exmb2.wvuh.wvuhs.com>
Content-Type: text/plain; charset="us-a

[Histonet] JOB OPENING

[Jeffrey Howery]

I am just putting this out there but we have an opening here at John C Lincoln 
Deer Valley Phoenix, Arizona.
If anyone might be interested please contact me. I can help fill you in on what 
we are looking for.
Thanks Jeff
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[Histonet] Commercial clearing and bluing solutions in Europe

[Jackie O'Connor]

I am looking for vendors in Germany who can provide commercially prepared, RTU 
H+E clarifier and bluing reagents.  Any advice?  Leica does not distribute 
these products in Germany, I've been told.
Thanks,
Jackie O'
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[Histonet] RE: Leica Bond IHC Platform

[Sue Hunter]

I've only been told that the Bond III could do a run in, say 3 hours, where the 
Bond Max might be done in 4.(don't take these times as true - just as an 
example).  We love the ability to do a delayed start so the slides are done 
when we come in  the next morning - especially the in-situ slides that take 
longer than the immunos.
sue

-Original Message-
From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] 
Sent: Thursday, April 05, 2012 10:51 AM
To: Sue Hunter; Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu
Subject: RE: Leica Bond IHC Platform

We have the Bond III. No complaints. I'm not sure what to compare to regarding 
speed, but we can do 4 runs a day if necessary. This does not include an 
overnight run, which we do as needed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Thursday, April 05, 2012 9:24 AM
To: Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica Bond IHC Platform

We have a Bond Max in my lab and love it!  It has been very dependable and easy 
to use . We also have three Ventana Ultras and two Lab Visions.  We originally 
got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors 
and a few other immunos on it.  Our pathologists felt the signal obtained with 
the DAB on the in-situ slides was superior to the NBT of the Ventana slides.  
The one draw back that Ventana will talk about is that you have three racks of 
ten slides - each rack is independent of the others, but not the continual load 
of the Ultras.  You also cannot mix pretreatments on each rack because of 
timing/heating issues.  But we have not found either of these two issues to be 
a problem for us.  The Bond III is supposed to be even faster than the Bond Max 
but haven't looked at that.
Sue

Sue Hunter
Supervisor, Advanced Diagnostics
Beaumont Health Systems
Royal Oak MI 48073
248-898-5146

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wellen, 
Terrence D. :LPH Lab
Sent: Wednesday, April 04, 2012 8:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond IHC Platform


Does anyone have any experience with this product?


Terrence Wellen HT(ASCP)
Legacy Good Samaritan Hospital
Portland, OR

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[Histonet] RE: Leica Bond IHC Platform

[Rathborne, Toni]

We have the Bond III. No complaints. I'm not sure what to compare to regarding 
speed, but we can do 4 runs a day if necessary. This does not include an 
overnight run, which we do as needed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Thursday, April 05, 2012 9:24 AM
To: Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Leica Bond IHC Platform

We have a Bond Max in my lab and love it!  It has been very dependable and easy 
to use . We also have three Ventana Ultras and two Lab Visions.  We originally 
got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors 
and a few other immunos on it.  Our pathologists felt the signal obtained with 
the DAB on the in-situ slides was superior to the NBT of the Ventana slides.  
The one draw back that Ventana will talk about is that you have three racks of 
ten slides - each rack is independent of the others, but not the continual load 
of the Ultras.  You also cannot mix pretreatments on each rack because of 
timing/heating issues.  But we have not found either of these two issues to be 
a problem for us.  The Bond III is supposed to be even faster than the Bond Max 
but haven't looked at that.
Sue

Sue Hunter
Supervisor, Advanced Diagnostics
Beaumont Health Systems
Royal Oak MI 48073
248-898-5146

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wellen, 
Terrence D. :LPH Lab
Sent: Wednesday, April 04, 2012 8:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond IHC Platform


Does anyone have any experience with this product?


Terrence Wellen HT(ASCP)
Legacy Good Samaritan Hospital
Portland, OR

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Re: [Histonet] Re: Massons Trichrome on decalcified bone

[Jack Ratliff]

Hello Sara!

Might I quickly ask why you decalcify instead of processing and embedding 
undecalcified using methyl methacrylate resin/plastic? I routinely process 
these type of specimens into MMA and cut either 5 micron sections without metal 
present or thick/ground sections polished to 30-40 microns if a metallic device 
is present.

These same specimens that have been processed and embedded into plastic and cut 
at 5 microns can be deplasticized prior to staining with H&E, Goldner's 
trichrome, VonKossa-MacNeal's tetrachrome, Safranin O-Fast Green, Sirius 
Red-Fast Green, etc. If these specimens contain a large metal implant, the 
sections are stained undeplasticized with Sanderson's Rapid Bone Stain & Van 
Gieson picrofuchsin.

Of course of you don't have these capabilities or equipment to process these 
specimens into resin, decalcification is your only option, but I would 
personally use 5% or 10% Formic acid instead of the lengthy EDTA process. I 
would also use methyl salicylate to replace the xylenes steps so that you can 
avoid making the bone too brittle and difficult to cut.

Feel free to message me back if you need further explanation or if you would 
even like to discuss privately by phone. I would also be happy to forward you 
stained images of these stains, previously listed and from this same specimen 
type you are working with, that has been resin embedded. Also, I have trained 
people all over the world to process undecalcified bone (any size) into 
resin/plastic and cut at 5 microns if this is an option for you!

Best Regards,

Jack

Jack Ratliff
Hard Tissue Histologist
Chairman, Hard Tissue Committee - National Society for Histotechnology 

On Apr 5, 2012, at 9:20 AM, Sara Landschoot  wrote:

> Hi Gayle,
> 
> Thanks so much for your response on Histonet. I have been getting several
> different answers that I have been trying to test.
> 
> I can give you more details on how we decal our bone here. We deal with
> entire sheep or primate spines which are grossed while frozen into slabs.
> The slabs are about 5-6mm thick. We take an initial xray to get a starting
> point then we decal the slabs in EDTA (after formalin fixation). Xrays are
> taken throughout the decal process to check for the endpoint.
> 
> As for post fixing in Bouins, I was using the water bath method (60
> degrees) for about an hour.
> 
> If you don't mind sending me the AFIP methods so I can try then out on my
> sections I would greatly appreciate it. My email is sllan...@gmail.com.
> 
> Again thank you for your help and I look forward to hearing from you.
> 
> Sara Landschoot
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> 

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[Histonet] RE: Leica Bond IHC Platform

[Sue Hunter]

We have a Bond Max in my lab and love it!  It has been very dependable and easy 
to use . We also have three Ventana Ultras and two Lab Visions.  We originally 
got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors 
and a few other immunos on it.  Our pathologists felt the signal obtained with 
the DAB on the in-situ slides was superior to the NBT of the Ventana slides.  
The one draw back that Ventana will talk about is that you have three racks of 
ten slides - each rack is independent of the others, but not the continual load 
of the Ultras.  You also cannot mix pretreatments on each rack because of 
timing/heating issues.  But we have not found either of these two issues to be 
a problem for us.  The Bond III is supposed to be even faster than the Bond Max 
but haven't looked at that.
Sue

Sue Hunter
Supervisor, Advanced Diagnostics
Beaumont Health Systems
Royal Oak MI 48073
248-898-5146

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wellen, 
Terrence D. :LPH Lab
Sent: Wednesday, April 04, 2012 8:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond IHC Platform


Does anyone have any experience with this product?


Terrence Wellen HT(ASCP)
Legacy Good Samaritan Hospital
Portland, OR

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[Histonet] Re: Massons Trichrome on decalcified bone

[Sara Landschoot]

Hi Gayle,

Thanks so much for your response on Histonet. I have been getting several
different answers that I have been trying to test.

I can give you more details on how we decal our bone here. We deal with
entire sheep or primate spines which are grossed while frozen into slabs.
The slabs are about 5-6mm thick. We take an initial xray to get a starting
point then we decal the slabs in EDTA (after formalin fixation). Xrays are
taken throughout the decal process to check for the endpoint.

As for post fixing in Bouins, I was using the water bath method (60
degrees) for about an hour.

If you don't mind sending me the AFIP methods so I can try then out on my
sections I would greatly appreciate it. My email is sllan...@gmail.com.

Again thank you for your help and I look forward to hearing from you.

 Sara Landschoot
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[Histonet] please don't sent e-mail

[mervatawad]

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