RE: [Histonet] vaginitis panel
I know. I was hoping to see what everyone else is doing. -Original Message- From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] Sent: Friday, May 04, 2012 6:30 PM To: Cynthia Pyse Cc: Subject: Re: [Histonet] vaginitis panel It's Friday :) Noticed none touched this? Sent from my iPhone On May 3, 2012, at 4:11 PM, "Cynthia Pyse" wrote: > Histonetters > > For those of you performing vaginitis panels, how are you billing > these? Do you bill for each separate test or use the combine CPT code? > We are having a discussion on what is our best method of billing. > Thanks in advance for the info. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 716-250-9235 etx. 232 > > e-mail cp...@x-celllab.com > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ken Marissael is out of the office
I will be out of the office starting 05/07/2012 and will not return until 05/11/2012. I will be away beginning today at our healthcare meeting and will return on Friday 5/11/12. My access to both e-mail and phone will be limited, so you may not receive an immediate response. Should you have an emergency, please contact customer service at 800-932-5000. Press 6 for Healthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] how often do you change your hematoxylin?
Meryl, We uses for more than 15 years a home made regressive hematoxylin Lillie. Our lab stains approximately 5000-6500 slides monthly. We does our stains maually. We never change our solution, but time to time add new hematoxylin for necessary level in stainig dish. Everyday we filter our working solution after workday. Never any metallic film on surface. Before hematoxyline we rinse rack with slides in DW 1 minute and drain excess water. Always very good results. Sincerely, Maxim Peshkov Russia, Taganrog. You wrote: We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: how often do you change your hematoxylin?
Meryl Roberts describes a vintage hematoxylin. I didn't know that hematoxylin came in vintages. But here's a poem by Oliver Wendell Holmes Sr., written about 1880, that makes the comparison. http://www.flickr.com/photos/bobrichmond/3866070073/in/set-72157622048353383 Holmes, a nearly forgotten American poet (he wrote "The Chambered Nautilus" and "The Wonderful One Hoss Shay") was a physician whose day job professor of anatomy at Harvard. There was some interest in lead-mordanted hematoxylins at that time, and he may have been working with them. Holmes actually published the poem as you see it on my Flickr link. What would now be called a concrete poem. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Neutrophil Antibodies
Hi John, In the past, I used Neutrophil Elastase from Dako on monkey FFPE tissue; no pretreatment needed; dilution with lot used = 1:50. Worked great. Jan Shivers UMN Vet Diag Lab On Sat, May 5, 2012 at 9:59 AM, John Shelley wrote: > Hi All in Histoland, > > Problem solving never ends! I am working up two antibodies for neutrophils > which are NIMP-R14 from Abcam which it is a rat monoclonal and also > Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I > have tried different conditions without any luck and was wondering if > anyone has worked with these antibodies either from Abcam or another > supplier and might have working conditions that have given results in the > past. We are trying this on FFPE sections which the antibodies say they > work on but I am thinking that I may try frozens. I was trying to avoid > this however because I am using these antibodies on mouse WAT. Any help > would be appreciated. Thanks!!! > > Happy Monday morning!!! > Kind Regards! > John J Shelley > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] how often do you change your hematoxylin?
We are a small lab and used Vintage for a while and loved the stain. I did notice that both the Eosin and Hemat did become watered down faster than any other brand we had tried and evaporated more. I also could only use it for about 2 wks before having to toss and refill. If it was more cost efficient we would still be using it. We went back to our Gill III and I filter it 2-3 times a week and can use it for about 3 weeks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Meryl Roberts Sent: Sunday, May 06, 2012 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how often do you change your hematoxylin? We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] how often do you change your hematoxylin?
Hi Meryl, What stain are you using your Hematoxylin for? We use Harris Hematoxylin for H&E staining and we filter it each day that we need to use it.We change it once per month. We are also not a high volume lab. We are a histology department in a biotech company. Your suspicions are right that it could be getting diluted by water dripping off the slides if they are not properly drained after the water rinsing step. Sheryl. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Meryl Roberts Sent: Sunday, May 06, 2012 4:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how often do you change your hematoxylin? We are currently using a vintage solution, and the manufacturer states that it should be good for 2500 slides. We are not a high volume lab; and currently I am finding that the longest I can stretch it out for is 2 weeks max, in which time we have stained less than 1000 slides. How often does on average do you change your hematoxylin? Do you think maybe it could be getting diluted by water dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
