Re: [Histonet] Fwd: Plants

2012-05-11 Thread Emily Sours
A canary? Well, I know what you used that for...how did that work out for
you?
Actually, I know the answer because you're alive.
EH&S should really look into this option, screw the badges...

Emily

The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson



On Fri, May 11, 2012 at 1:32 PM, William  wrote:

> I have had plants in a number of labs. Could be against the rules, but I
> never saw it. I even had a canary in one lab - pretty sure that is against
> the rules.
>
> Will Chappell
>
> Sent from my iPhone
>
> On May 11, 2012, at 1:29 PM, "Behnaz Sohrab"  wrote:
>
> >
> > I was told by infectious control person that plants are not allowed in
> the lab?? IS this true? any experience with this?
> > Thank you, Behnaz
> > ___
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> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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RE: [Histonet] Fwd: Plants

2012-05-11 Thread Sebree Linda A
Yeah but they used to use canaries in mines to warn of toxic levels of
gases; having one in a histo lab might be a VERY good idea! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Sent: Friday, May 11, 2012 12:33 PM
To: Behnaz Sohrab
Cc: 
Subject: Re: [Histonet] Fwd: Plants

I have had plants in a number of labs. Could be against the rules, but I
never saw it. I even had a canary in one lab - pretty sure that is
against the rules. 

Will Chappell

Sent from my iPhone

On May 11, 2012, at 1:29 PM, "Behnaz Sohrab"  wrote:

> 
> I was told by infectious control person that plants are not allowed in
the lab?? IS this true? any experience with this?
> Thank you, Behnaz
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Fwd: Plants

2012-05-11 Thread Sebree Linda A
I've always had at least one; makes the day more tolerable. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz
Sohrab
Sent: Friday, May 11, 2012 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: Plants


I was told by infectious control person that plants are not allowed in
the lab?? IS this true? any experience with this?
Thank you, Behnaz

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RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread gayle callis
You are also  likely to have spheres dissolve in toluene based mounting
media.   Aqueous media may be the answer unless the neutral red runs out of
the tissue in the aqueous environment.   Hard to get the best of both worlds
if dye runs in aqueous media and spheres dissolve in solvent based .   Good
luck! 

Gayle Callis 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, May 11, 2012 12:23 PM
To: histonet@lists.utsouthwestern.edu; Andrew Coleman
Subject: Re: [Histonet] Xylene Substitute for Counterstain Clearing

After staining dry the sections in an oven at 60ºC for 10 minutes. When
completely dried, coverslip as usual. Beware of the mounting medium solvent
because it may contain xylene as well. Use one mounting medium without
xylene.
René J.

--- On Fri, 5/11/12, Andrew Coleman  wrote:


From: Andrew Coleman 
Subject: [Histonet] Xylene Substitute for Counterstain Clearing
To: histonet@lists.utsouthwestern.edu
Date: Friday, May 11, 2012, 12:25 PM


Hi all,

We are performing a neutral red counterstain on tissue sections containing
colored polystyrene microspheres. The spheres are inert to alcohol, but are
washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and methylene chloride for what its worth.

Is it reasonable to coverslip these slides in permanent mount without
clearing with xylene after dehydrating the tissue? Or does anyone know of a
substitute clearing agent with chemical properties dissimilar enough from
xylene that might be worth trying instead?

Thanks,

Andrew

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Re: [Histonet] Fwd: Plants

2012-05-11 Thread Kim Donadio
Hate to say it, but yes plants are considered infectious. Thats why you cant 
take them in ICU's either. I guess the mold or bacterias can grow on them. Most 
places let this slide, but some dont. Good luck! 




From: William 
To: Behnaz Sohrab  
Cc: ""  
Sent: Friday, May 11, 2012 1:32 PM
Subject: Re: [Histonet] Fwd: Plants

I have had plants in a number of labs. Could be against the rules, but I never 
saw it. I even had a canary in one lab - pretty sure that is against the rules. 

Will Chappell

Sent from my iPhone

On May 11, 2012, at 1:29 PM, "Behnaz Sohrab"  wrote:

> 
> I was told by infectious control person that plants are not allowed in the 
> lab?? IS this true? any experience with this?
> Thank you, Behnaz
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] Fwd: Plants

2012-05-11 Thread Lucie Guernsey
I keep a pothos and spider plant in my lab. EH&S has never complained,
though I can't say one way or another if it's technically allowed.

While my plants are mostly just decorative (I don't think I have enough of
them to make much of a difference), it doesn't hurt that they may be
filtering our air somewhat. NASA compiled a list of air-filtering plants
that can eliminate significant amounts of formaldehyde, xylene, benzene,
etc. (Source:   http://en.wikipedia.org/wiki/List_of_air-filtering_plants).

Lucie
UCSD
Dept. of Pathology


On Friday, May 11, 2012, Victoria Baker wrote:

> It's probably more toxic for the plants, but I like having them and no one
> has told me I had to remove them.  Ivy's are the most sturdy and the green
> color just perks up things.
> On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab  wrote:
>
> >
> > I was told by infectious control person that plants are not allowed in
> the
> > lab?? IS this true? any experience with this?
> > Thank you, Behnaz
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> ___
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>
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RE: [Histonet] paraffin melting in VIP

2012-05-11 Thread Goins, Tresa
When it is time to exchange reagents, the paraffin is the first thing we do 
because of the melting time required.
We fill up the bin and place it in the oven until there is room to add more wax 
pellets - we do not stuff it in, but leave it rather loose.  We do this about 
six times during the day until the bin is full.
The paraffin pellets are not "cold" and there is a lot of residual heat in the 
oven due to the three hot wax bins already at temperature, so I am not sure how 
much "stress" this places on the heating elements.  

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Friday, May 11, 2012 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] paraffin melting in VIP

HI!

A question for those, who melt the paraffin directly in the VIP. How long does 
it take to melt the pellets in the VIP-oven?

 

Thanks

Gudrun

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AW: [Histonet] paraffin melting in VIP

2012-05-11 Thread Gudrun Lang
Thanks for your kind responses!
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Freitag, 11. Mai 2012 19:59
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] paraffin melting in VIP

HI!

A question for those, who melt the paraffin directly in the VIP. How long
does it take to melt the pellets in the VIP-oven?

 

Thanks

Gudrun

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RE: [Histonet] paraffin melting in VIP

2012-05-11 Thread Sarah Dysart
This is probably a best case scenario, but for labs like the one I am currently 
in I melt it in the processor.  My lab doesn't have the funds to buy me a 
melting pot (I have had them in all my other labs, just don't know what they 
are technically called).
To answer your question it usually takes overnight and it's melted, but 
sometimes I end up having to add a little more to get to top off level, that 
takes a couple hours.
Good Luck!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, May 11, 2012 1:28 PM
To: histonet@lists.utsouthwestern.edu; gu.l...@gmx.at
Subject: Re: [Histonet] paraffin melting in VIP

Regardless of the time it takes or of how many people do it, melting the 
paraffin directly in the VIP should not be done because it causes the heating 
elements to work extra reducing their useful life. They are quite expensive to 
replace!. 
Melt the paraffin outside the VIP and use the VIP only to keep the melted 
paraffin at the temperature you desire.
René J.

--- On Fri, 5/11/12, Gudrun Lang  wrote:


From: Gudrun Lang 
Subject: [Histonet] paraffin melting in VIP
To: histonet@lists.utsouthwestern.edu
Date: Friday, May 11, 2012, 1:58 PM


HI!

A question for those, who melt the paraffin directly in the VIP. How long
does it take to melt the pellets in the VIP-oven?



Thanks

Gudrun

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Re: [Histonet] paraffin melting in VIP

2012-05-11 Thread Jennifer Campbell
I agree Rene!

 I also believe Sakura recommends not putting paraffin flakes directly in
the containers.

On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa  wrote:

> Regardless of the time it takes or of how many people do it, melting the
> paraffin directly in the VIP should not be done because it causes the
> heating elements to work extra reducing their useful life. They are quite
> expensive to replace!.
> Melt the paraffin outside the VIP and use the VIP only to keep the melted
> paraffin at the temperature you desire.
> René J.
>
> --- On Fri, 5/11/12, Gudrun Lang  wrote:
>
>
> From: Gudrun Lang 
> Subject: [Histonet] paraffin melting in VIP
> To: histonet@lists.utsouthwestern.edu
> Date: Friday, May 11, 2012, 1:58 PM
>
>
> HI!
>
> A question for those, who melt the paraffin directly in the VIP. How long
> does it take to melt the pellets in the VIP-oven?
>
>
>
> Thanks
>
> Gudrun
>
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>



-- 
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137


IMPORTANT NOTICE:  This e-mail and any attachments may contain confidential
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Re: [Histonet] paraffin melting in VIP

2012-05-11 Thread Rene J Buesa
Regardless of the time it takes or of how many people do it, melting the 
paraffin directly in the VIP should not be done because it causes the heating 
elements to work extra reducing their useful life. They are quite expensive to 
replace!. 
Melt the paraffin outside the VIP and use the VIP only to keep the melted 
paraffin at the temperature you desire.
René J.

--- On Fri, 5/11/12, Gudrun Lang  wrote:


From: Gudrun Lang 
Subject: [Histonet] paraffin melting in VIP
To: histonet@lists.utsouthwestern.edu
Date: Friday, May 11, 2012, 1:58 PM


HI!

A question for those, who melt the paraffin directly in the VIP. How long
does it take to melt the pellets in the VIP-oven?



Thanks

Gudrun

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Re: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Rene J Buesa
After staining dry the sections in an oven at 60ºC for 10 minutes. When 
completely dried, coverslip as usual. Beware of the mounting medium solvent 
because it may contain xylene as well. Use one mounting medium without xylene.
René J.

--- On Fri, 5/11/12, Andrew Coleman  wrote:


From: Andrew Coleman 
Subject: [Histonet] Xylene Substitute for Counterstain Clearing
To: histonet@lists.utsouthwestern.edu
Date: Friday, May 11, 2012, 12:25 PM


Hi all,

We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres are inert to
alcohol, but are washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and methylene chloride for what its worth.

Is it reasonable to coverslip these slides in permanent mount without
clearing with xylene after dehydrating the tissue? Or does anyone know
of a substitute clearing agent with chemical properties dissimilar
enough from xylene that might be worth trying instead?

Thanks,

Andrew

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Re: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Victoria Baker
I think that it is called Crystal Mount - but apply to section, allow to
harden dip slide in clearing media and coverslip.  I know that there must
be others out there as well.
Good Luck



On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman  wrote:

> Hi all,
>
> We are performing a neutral red counterstain on tissue sections
> containing colored polystyrene microspheres. The spheres are inert to
> alcohol, but are washed out when we clear with xylene to coverslip.
> The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
> chloroform and methylene chloride for what its worth.
>
> Is it reasonable to coverslip these slides in permanent mount without
> clearing with xylene after dehydrating the tissue? Or does anyone know
> of a substitute clearing agent with chemical properties dissimilar
> enough from xylene that might be worth trying instead?
>
> Thanks,
>
> Andrew
>
> ___
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>
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Re: [Histonet] Fwd: Plants

2012-05-11 Thread Victoria Baker
It's probably more toxic for the plants, but I like having them and no one
has told me I had to remove them.  Ivy's are the most sturdy and the green
color just perks up things.
On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab  wrote:

>
> I was told by infectious control person that plants are not allowed in the
> lab?? IS this true? any experience with this?
> Thank you, Behnaz
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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[Histonet] paraffin melting in VIP

2012-05-11 Thread Gudrun Lang
HI!

A question for those, who melt the paraffin directly in the VIP. How long
does it take to melt the pellets in the VIP-oven?

 

Thanks

Gudrun

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RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Cynthia Pyse
Andrew
You could use Clearium from Leica. Clearium can either be coverslipped from
xylene or isopropyl alcohol. Drying time from isopropyl is longer then
xylene.
Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew
Coleman
Sent: Friday, May 11, 2012 12:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute for Counterstain Clearing

Hi all,

We are performing a neutral red counterstain on tissue sections containing
colored polystyrene microspheres. The spheres are inert to alcohol, but are
washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and methylene chloride for what its worth.

Is it reasonable to coverslip these slides in permanent mount without
clearing with xylene after dehydrating the tissue? Or does anyone know of a
substitute clearing agent with chemical properties dissimilar enough from
xylene that might be worth trying instead?

Thanks,

Andrew

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Re: [Histonet] Fwd: Plants

2012-05-11 Thread William
I have had plants in a number of labs. Could be against the rules, but I never 
saw it. I even had a canary in one lab - pretty sure that is against the rules. 

Will Chappell

Sent from my iPhone

On May 11, 2012, at 1:29 PM, "Behnaz Sohrab"  wrote:

> 
> I was told by infectious control person that plants are not allowed in the 
> lab?? IS this true? any experience with this?
> Thank you, Behnaz
> ___
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] Fwd: Plants

2012-05-11 Thread Behnaz Sohrab

I was told by infectious control person that plants are not allowed in the 
lab?? IS this true? any experience with this?
Thank you, Behnaz
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RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Elizabeth Chlipala
Andrew

There are aqueous permanent mounting medias such as Advantage Permanent 
Mounting Media from Accurate Chemical NB300A (516) 333-2221 its been years 
since I used it but it does work on some applications.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Coleman
Sent: Friday, May 11, 2012 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute for Counterstain Clearing

Hi all,

We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres are inert to
alcohol, but are washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and methylene chloride for what its worth.

Is it reasonable to coverslip these slides in permanent mount without
clearing with xylene after dehydrating the tissue? Or does anyone know
of a substitute clearing agent with chemical properties dissimilar
enough from xylene that might be worth trying instead?

Thanks,

Andrew

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[Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Andrew Coleman
Hi all,

We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres are inert to
alcohol, but are washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and methylene chloride for what its worth.

Is it reasonable to coverslip these slides in permanent mount without
clearing with xylene after dehydrating the tissue? Or does anyone know
of a substitute clearing agent with chemical properties dissimilar
enough from xylene that might be worth trying instead?

Thanks,

Andrew

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RE: [Histonet] Re. Grossing Cassette Baskets

2012-05-11 Thread Morken, Timothy
You can get the old-style sakura baskets from this company. These are the ones 
in which the plastic cassettes lay on their sides.  They are about half the 
price. 

*

http://store.techonebiomedical.com/store/

We sell the old style Sakura baskets. We have the 50 and 100 cassette models. 
We also sell a 150 cassette deluxe model. 

Thanks
Matt

--
Matthew Mincer
Tech One Biomedical Services
159 N Marion Street, PMB163
Oak Park, IL 60301
(708) 383-6040 X 10
fax (708) 383-6045
cell (708) 822-3738



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell Wan
Sent: Thursday, May 10, 2012 3:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re. Grossing Cassette Baskets 

Hi James,
I am interested in locating some baskets as well.
I am tempted to go to a local metal works shop and ask them to make some 
stainless steel ones for myself. It will be approx. 1/10 of the price.



Regards
Mitchell Wan

InfinityPATH Pty Ltd

M: 0418 745 750
P: 07 3123 
F: 07 3123 8889

E: mitchell@infinitypath.com.au

W: www.infinitypath.com.au


Does anybody know where we can purchase wire or plastic tissue cassette baskets 
for our grossing stations.  We are looking for baskets that will keep the 
cassettes in line similar to the old VIP 3000 metal baskets with dividers.  In 
our workflow we later transfer the cassettes to processing
baskets.   (Looked into purchasing more processing baskets, but at 400-500 a
basket that doesn't seem to feasible.)  Surely someone has old wire baskets or 
new plastic ones.  Thanks for your help.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046


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Re: [Histonet] cryptosopridia

2012-05-11 Thread Richard Cartun
Yes, most testing is performed in the Microbiology Laboratory; however, I have 
an IHC test for formalin-fixed, paraffin-embedded tissue.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Webb, Dorothy L"  5/11/2012 10:11 AM >>>
What stain is anyone using for cryptosporidia in histology??  I thought this 
was more of a microbiology test.  Could use some help please!!

Thanks,

Dorothy Webb



  
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[Histonet] cryptosopridia

2012-05-11 Thread Webb, Dorothy L
What stain is anyone using for cryptosporidia in histology??  I thought this 
was more of a microbiology test.  Could use some help please!!

Thanks,

Dorothy Webb



  
This e-mail and any files transmitted with it are confidential and are intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient or the individual responsible for delivering 
the e-mail to the intended recipient, please be advised that you have received 
this e-mail in error and that any use, dissemination, forwarding, printing, or 
copying of this e-mail is strictly prohibited.

If you have received this communication in error, please return it to the 
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