[Histonet] Histology Technologist 2, new position at Northwestern University, Chicago Campus
Job Title: Histology Technologist 2 Job ID: 19392 Department: Cancer Center Northwestern University, Chicago Campus This is a newly created position in a small Core Laboratory that offers histology service University wide for both campuses. Apply online by following the information and link below. This is a great position located in the heart of beautiful downtown Chicago. Apply on the Northwestern University Careers site by going to: http://www.northwestern.edu/hr/jobs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Preferences between kits for EBER
Biocare has a kit. My class used it successfully. On Aug 10, 2012, at 1:46 PM, "Lewis, Patrick" wrote: > Hi Everyone, > > Thanks for the response earlier. > > Does anyone any advice on EBER ISH Kits? Specifically, if they had better > results, or less problems with one brand vs another? > Right now I am considering both Novacastra's kit for 970.00 and Vector Labs > kit for 905.00 > Has anyone used these kits? Has anyone tried a different kit they could > recommend? > > Thanks > > Patrick. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Preferences between kits for EBER
Hi Everyone, Thanks for the response earlier. Does anyone any advice on EBER ISH Kits? Specifically, if they had better results, or less problems with one brand vs another? Right now I am considering both Novacastra's kit for 970.00 and Vector Labs kit for 905.00 Has anyone used these kits? Has anyone tried a different kit they could recommend? Thanks Patrick. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sections falling off in day 2 ISH (Megha Kumar)
Hi Megha, Not sure why your sections are falling off. When I did ISH I used Colorfrost Plus Slides. I would cut at 4 microns-mouse or human. I would dry them at room temp a few hours followed by 55 C for an hour. I would use the slides the next day or next week, never the same day. On day 2 my SSC was 60 C and I used a stir bar for 2 hours on slow stirring. Rarely did I lose any sections. Terri Thinnes Research Assistant The Scripps Research Institute La Jolla, CA Message: 13 Date: Fri, 10 Aug 2012 14:40:30 +0530 From: Megha Kumar Subject: [Histonet] sections falling off in day 2 ISH To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone suggest me why this is happening? How to prevent the slides from falling off on day 2? Thank you for all the help! regards Megha * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] keeping acids in the lab.
We, of course, keep them separate from bases. We don't have large amounts, so we keep them in cabinet under the hood. Do not keep in flammable cabinets. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Friday, August 10, 2012 1:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] keeping acids in the lab. Hello Histonet, How are you keeping daily used acids like hydrochloric, nitric, acetic and formic? Thank you.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Has anyone done ISH with fairly large FFPE sections?
We buy rubber cement from walmart or staples and seal the outside of the coverslip before the hybridization step. And we have had much success with minimal probe. But I understand, I cross my fingers when I do large resected pieces of liver. AND I keep on complaining to the vendors that they do not provide enough probe to cover paraffin sections of large size. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick [patrick.le...@seattlechildrens.org] Sent: Friday, August 10, 2012 11:57 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Has anyone done ISH with fairly large FFPE sections? Hi Everyone, I am going to do some ISH for EBER on FFPE sections, and I have some concerns on the logistics. The kits I am looking at are for 50 rxns, and it seems like they expect to use 20 uL probe per rxn. My sections are rather large, covering about 2/3 of a standard slide. How many uL will it take to make sure I get good coverage and have enough to avoid drying out the slide? I plan to cover slip them and use a humidity chamber, but even still, I am worried that with such a small volume it don't be enough. These kits are expensive, +$900.00 each and if I have to double/triple my rxn volume that would use up the kit in a hurry. I haven't fully familiarized myself with the protocol yet, so maybe my concerns are unjustified, but if anyone has done some ISH, I'd appreciate any advice you'd care to give. Thanks Patrick. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] keeping acids in the lab.
Hello Histonet, How are you keeping daily used acids like hydrochloric, nitric, acetic and formic? Thank you.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Has anyone done ISH with fairly large FFPE sections?
Hi Everyone, I am going to do some ISH for EBER on FFPE sections, and I have some concerns on the logistics. The kits I am looking at are for 50 rxns, and it seems like they expect to use 20 uL probe per rxn. My sections are rather large, covering about 2/3 of a standard slide. How many uL will it take to make sure I get good coverage and have enough to avoid drying out the slide? I plan to cover slip them and use a humidity chamber, but even still, I am worried that with such a small volume it don't be enough. These kits are expensive, +$900.00 each and if I have to double/triple my rxn volume that would use up the kit in a hurry. I haven't fully familiarized myself with the protocol yet, so maybe my concerns are unjustified, but if anyone has done some ISH, I'd appreciate any advice you'd care to give. Thanks Patrick. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin
Do you recycle your alcohols? If you do and you put the recycled alcohols at the end (running down to xylene) it can GREATLY decolorize the eosin in the H&E stain. Also, make sure you have a good rinse after the blueing step as that can mess with the eosin's pH which will also decrease staining. -Original message- From: "Hannen, Valerie" valerie.han...@parrishmed.com Date: Thu, 09 Aug 2012 09:23:26 -0500 To: histonet histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin > Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one > > day to the next...one day it looks great...the next it is weak!! > > I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the properconcentrations3) > > reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing > > steps. 5) I have checked the pH of the water as well. > > Any help and suggestions would greatly appreciated!! > > Thanks Gang!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.han...@parrishmed.com > > > > > > > > * > > > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidentialor otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Brendal C. Finlay, HT (ASCP) West Florida Medical Center Clinic brendal.fin...@medicalcenterclinic.com phone - 850.474.8758 fax - 850.474.8584 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sections falling off in day 2 ISH
Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone suggest me why this is happening? How to prevent the slides from falling off on day 2? Thank you for all the help! regards Megha * * * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
