[Histonet] Twort's Gram
We are having no end of trouble with our Twort's Gram. Can someone share protocol, tip and tricks Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] question(s)
The sectioning protocols/sequences are determined by the pathologists and what they think is required to make diagnoses. We also stored the slides with extras sections until the case is signed and get an additional OK from the signing pathologist before disposing of the unused sections. I would never ever place ribbons of sections on paper. That is calling for "disaster" in the event that some sections are required. If you think there is too much extra work and materials wastes your only solution is to ask the pathologists to review their requirements as to extra sections, specially those the pathologist may want to use for IHC. In some protocols the pathologists require automated IHC procedures before signing the case. All these issues are to be resolved by the pathologists. If the excess work is too much, you can always talk with your manager to get help regarding the protocols. René J. From: "Webb, Dorothy L" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, December 7, 2012 1:10 PM Subject: [Histonet] question(s) How does everyone handle storing extra paraffin sections that are cut as a standard on certain protocols, such as prostate needle bx's? We currently place them on a slide and save until the case is signed out. I am concerned with the amount of waste and cost with the way we are doing it and would like other ideas. If you keep the ribbons laid out on paper, do you stack the sheets of paper and is that problematic when it comes time to need that certain cut for IHC? Also, please let me know of those who have the VIP 6, how you like it, pros and cons and also the Leica ASP300S. Would greatly appreciate all feedback on both requests and thank you so very much!! Dorothy Webb , HT(ASCP) Regions Histology Technical Specialist This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION
Yes you are. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, December 07, 2012 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION Hi All: First, thank you for all your feedback on the processors. I site-visited a VIP6 at a local hospital From my understanding the VIP6 can rotate absolute alcohol and xylene (using the bulk reservoirs), as well as the paraffin stations, but it cannot rotate other concentrations of alcohol based on hydrometer readings. Am I correct in this appraisal? Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Message 2: dako her2
Check www.cqpath.com for more info Verstuurd vanaf mijn iPad Op 7 dec. 2012 om 19:03 heeft "histonet-requ...@lists.utsouthwestern.edu" het volgende geschreven: > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to >histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at >histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. How to open Reichert 820? (Jon Krupp) > 2. DAKO Her2 (Joanne Clark) > 3. Embedding Centers (Tim Wheelock) > 4. RE: Embedding Centers (O'Donnell, Bill) > 5. Paraformaldehyde Solution Recipe for Perfusion (Andrew Coleman) > 6. Re: automated microtomes (Jay Lundgren) > 7. RE: Embedding Centers (Burton, Lynn) > 8. RE: automated microtomes (Rathborne, Toni) > 9. refurbished histology equipment (Patsy Ruegg) > 10. Alcian Blue (Sheila Adey) > 11. RE: Alcian Blue (susan.wal...@hcahealthcare.com) > 12. RE: Alcian Blue (Lynette Pavelich) > 13. Re: Alcian Blue (Rene J Buesa) > 14. Re: Paraformaldehyde Solution Recipe for Perfusion (Geoff) > 15. TISSUE PROCESSOR FOLLOW-UP QUESTION (Tim Wheelock) > > > -- > > Message: 1 > Date: Thu, 6 Dec 2012 10:10:31 -0800 > From: Jon Krupp > Subject: [Histonet] How to open Reichert 820? > To: HISTONET > Message-ID: <09ffdb2c-3cad-4342-b6bb-4a5299d72...@deltacollege.edu> > Content-Type: text/plain; charset=us-ascii > > Hi > > Anyone know how to open the cover of a Reichert 820 microtome? > > This is the model that has a wheel for the coarse advance on the left side. > > I am used to the AO Style, pop the latch & tip it back. These usually have a > crank and a cut out slot on the left. > > This one uses 4 screws from the bottom to secure the lid and there is no cut > out for the coarse advance to slide through. Looks like the wheel has to be > removed to remove the cover. Getting the wheel off is where I am stuck. > > This is an old microtome we have had sitting around, would like to check the > guts and clean it up. > > Jon > > > Jonathan Krupp > Applied Science, Business & Technology > San Joaquin Delta College > 5151 Pacific Ave. > Stockton, CA 95207 > 209-954-5284 > jkr...@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > > > > > -- > > Message: 2 > Date: Thu, 6 Dec 2012 18:32:49 + > From: Joanne Clark > Subject: [Histonet] DAKO Her2 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: ><0494a7d4e8cc254ea2fb81464982e37894637...@s10maild001n4.sh10.lan> > Content-Type: text/plain; charset="us-ascii" > > > Hi All, sorry for how I am submitting this but I have been sending in > questions to the address provided by nothing is going through. > I would like to know who uses DAKO's IVD Her2 IHC marker and how it works. > We want to start running this on our Leica BOND and any info would be much > appreciated. > > Thanks > Joanne Clark, HT(ASCP) > Histology Supervisor > Pathology Consultants of New Mexico > Roswell, NM > > > > -- > > Message: 3 > Date: Thu, 06 Dec 2012 15:28:50 -0500 > From: Tim Wheelock > Subject: [Histonet] Embedding Centers > To: histonet@lists.utsouthwestern.edu > Message-ID: <50c10002.8070...@mclean.harvard.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi Everyone: > > I am also in the market for a new paraffin embedding center. > I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and > the Thermo-Fisher HistoStar. > I was wondering if people could give me their critical opinion on these, > or other machines. > What sorts of problems have you had with them. > > I currently have a 25 year old Shandon Embedding Center. I like it a lot. > But I would like to find a machine with a specimen holding tank large > enough to allow me to immerse 300 cassettes all at once. > This is because I infiltrate brain tissue with Tissue Path Paraplast but > embed with Surgipath Embedding Media > So I let the cassettes sit immersed in the Surgipath for an hour or two > before embedding. > > (Until I can buy a new processor, The Shandon's holding tank also serves > as a third processing station, since my Shandon Hypercenter has only 2 > wax reservoirs) > I also do not feel comfortable having the cassettes sitting dry in the > holding tank > > Thanks, > > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > > > -- > > Message: 4 > Date: Thu, 6 Dec 2012 13:41:28 -0700 > From: "O'Do
[Histonet] question(s)
How does everyone handle storing extra paraffin sections that are cut as a standard on certain protocols, such as prostate needle bx's? We currently place them on a slide and save until the case is signed out. I am concerned with the amount of waste and cost with the way we are doing it and would like other ideas. If you keep the ribbons laid out on paper, do you stack the sheets of paper and is that problematic when it comes time to need that certain cut for IHC? Also, please let me know of those who have the VIP 6, how you like it, pros and cons and also the Leica ASP300S. Would greatly appreciate all feedback on both requests and thank you so very much!! Dorothy Webb , HT(ASCP) Regions Histology Technical Specialist This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION
Hi All: First, thank you for all your feedback on the processors. I site-visited a VIP6 at a local hospital From my understanding the VIP6 can rotate absolute alcohol and xylene (using the bulk reservoirs), as well as the paraffin stations, but it cannot rotate other concentrations of alcohol based on hydrometer readings. Am I correct in this appraisal? Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraformaldehyde Solution Recipe for Perfusion
What issues are you having? That might help the list diagnose the problem. Geoff On 12/6/2012 3:43 PM, Andrew Coleman wrote: Hi all, We are performing transcardial perfusions in rats using paraformaldehyde in 0.1M potassium phosphate buffer. Can anyone think of any issues that would be caused by using phosphate buffer made from solely potassium salts (basic and dibasic), rather than a mixture of sodium and potassium or only the sodium salts? We do our rinse with 0.1 M PB + Saline and then follow up with solutions just made up in the potassium phosphate buffer (therefore no Na+). Could this cause any tonicity/osmolarity issues? We are trying to troubleshoot some issues we are having with the perfused tissue. Thanks! - Andrew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alcian Blue
If after many successful runs with your Alcian Blue reagents you get a complaint about either strength of staining or cells not staining at all, most likely you have a problem with the solutions. If you prepare them "in house" check the preparation date and prepare a fresh solution. The general procedure calls for 30 min, unless you use a microwave oven version of the staining protocol. René J. From: Sheila Adey To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, December 6, 2012 8:58 PM Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alcian Blue
30 here. Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of susan.wal...@hcahealthcare.com [susan.wal...@hcahealthcare.com] Sent: Friday, December 07, 2012 3:04 AM To: sa...@hotmail.ca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Alcian Blue We've always used 30 minutes and it has never failed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Thursday, December 06, 2012 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alcian Blue
We've always used 30 minutes and it has never failed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Thursday, December 06, 2012 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
