RE: [Histonet] Inking esophagus biopsies

2012-12-11 Thread Mike Pence
I use eosin in a squirt bottle.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila
Adey
Sent: Tuesday, December 11, 2012 2:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Inking esophagus biopsies



Hi Everyone:Can anyone reccommend a good way to ink esophageal biopsies
without using mercurochrome? ThanksSheila
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Inking esophagus biopsies

2012-12-11 Thread Sheila Adey

Hi Everyone:Can anyone reccommend a good way to ink esophageal biopsies without 
using mercurochrome? ThanksSheila 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Pathologist Consult

2012-12-11 Thread Sarah Dysart
Does anyone know of any good services that do a per slide pathology read out?  
I would prefer a veterinary pathologist and it would not be a very large case 
load.  Mainly tox. related reports would be what we need generated.
Thanks

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Recall: Node Issue

2012-12-11 Thread Metzger, Kenneth
Metzger, Kenneth would like to recall the message, "Node Issue".

 ---
The information transmitted by this e-mail and any included
attachments are from ARUP Laboratories and are intended only for the
recipient. The information contained in this message is confidential
and may constitute inside or non-public information under
international, federal, or state securities laws, or protected health
information and is intended only for the use of the recipient.
Unauthorized forwarding, printing, copying, distributing, or use of
such information is strictly prohibited and may be unlawful. If you
are not the intended recipient, please promptly delete this e-mail
and notify the sender of the delivery error or you may call ARUP
Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
(800) 522-2787 ext. 2100


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Recall: Node Issue

2012-12-11 Thread Metzger, Kenneth
Metzger, Kenneth would like to recall the message, "Node Issue".

 ---
The information transmitted by this e-mail and any included
attachments are from ARUP Laboratories and are intended only for the
recipient. The information contained in this message is confidential
and may constitute inside or non-public information under
international, federal, or state securities laws, or protected health
information and is intended only for the use of the recipient.
Unauthorized forwarding, printing, copying, distributing, or use of
such information is strictly prohibited and may be unlawful. If you
are not the intended recipient, please promptly delete this e-mail
and notify the sender of the delivery error or you may call ARUP
Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
(800) 522-2787 ext. 2100


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Enquiry about blood removal from frozen tissue sections

2012-12-11 Thread Sue Hunter
You could try hydrogen peroxide - it wil get rid of the heme in the RBCs which 
is probably giving you the background.  I would try a 1:10 dilution (or higher) 
 of the H2O2 (in PBS) that you usually get from the store/pharmacy - undiluted 
will probably bubble too much and lift off the tissue.  You will have to try 
dilutions and time - usually 10 minutes is good, but with a diluted solution 
you may need to go longer.  Then wash the slides in PBS to get rid of the H2O2.
Good Luck
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of girish cm
Sent: Tuesday, December 11, 2012 12:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Enquiry about blood removal from frozen tissue sections

Hello,

 I would like to know whether any methods (other than cold PBS
wash) or products are available to remove the blood components from frozen 
tissue sections. My application is antibody targeted spectroscopic imaging in 
which the blood components are providing background noise.

Thanking you,

Regards
--
Girish C M
Senior Research Fellow
Amrita Centre for Nanoscience & Molecular Medicine Amrita Institute of Medical 
Sciences & Research Centre Cochin, Kerala
India-682 041
Ph: 9645095045
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet