Re: [Histonet] Voice Recognition Systems

[Cristi Rigazio]

We use Dragon Speak, our LIS is WindoPath.
Cristi

Sent from my iPhone

On Jan 15, 2013, at 3:37 PM, Ann Specian  wrote:

> 
> I am looking for a voice recognition system for the gross room.  It needs to 
> be a system that will interface with our LIS. 
> 
> Does anyone have any recommendations in regard to vendors?
> 
> Thank you,
> Ann Specian
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[Histonet] Voice Recognition Systems

[Ann Specian]

I am looking for a voice recognition system for the gross room.  It needs to be 
a system that will interface with our LIS. 

Does anyone have any recommendations in regard to vendors?

Thank you,
Ann Specian
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[Histonet] Jack Ratliff & Dr. Heiko Richter on HistoTALK

[David Kemler]

Hi Folks -

Laser Hisology?! That's what is being discussed (Part 1) by Jack Ratliff, 
Ratliff Histology Consultants, Franklin, TN and Dr. Heiko Richter, ROWIAK 
Company, Hannover, Germany on HistoTALK http://www.histotalk.com/. Very 
interesting...very interesting in deed! This is a definite must hear!

Yours,
Dave 
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Re: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining

[Rene J Buesa]

Gudrun:
Were the cases to be known as positive, or were you trying to determine just 
that?
If they were known as (+) then the bacteria should be seen outside the cells.
If you were trying to determine if they were (+) and you do not see the 
organisms it is just that those cases were (-), especially if all were stained 
in a single batch.
I think it would be very odd if the organisms are not seen but they generated 
so much toxin that it went into the cells.
René J.

From: Gudrun Lang 
To: Histonet@lists.utsouthwestern.edu 
Sent: Tuesday, January 15, 2013 3:56 PM
Subject: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic 
staining

Hi!
I need some help with the interpretation of Hp-IHC. I stained several cases
of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200,
ventana benchmark).
Some cases showed the nice bacteria and had usually a clean overall
background. But some cases showed no bacteria but slight cellular staining
of epithelial cells. 
It was confined on the cells, and usually not all cells in the biopsy had
this staining.

>From literature I learned, that Hp brings its toxine and other proteins
(CagA) into the hostcell. Is it possible, that the polyclonal antibody
detects this stuff, although hardly an intact bacterium can be seen?
Anybody with similar findings?

Thanks in advance
Gudrun Lang


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[Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining

[Gudrun Lang]

Hi!
I need some help with the interpretation of Hp-IHC. I stained several cases
of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200,
ventana benchmark).
Some cases showed the nice bacteria and had usually a clean overall
background. But some cases showed no bacteria but slight cellular staining
of epithelial cells. 
It was confined on the cells, and usually not all cells in the biopsy had
this staining.

>From literature I learned, that Hp brings its toxine and other proteins
(CagA) into the hostcell. Is it possible, that the polyclonal antibody
detects this stuff, although hardly an intact bacterium can be seen?
Anybody with similar findings?

Thanks in advance
Gudrun Lang


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[Histonet] (no subject)

[Galina Deyneko]

Hi Betsy
We use the Thermo Shandon precision Cut paraffin cat. # B1002490 ( Thermo 
Scientific)


Galina Deyneko
Novartis, Cambridge, MA
 
617-871-7613 w
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[Histonet] HPV IHC

[Marian Powers]

Hi:  Has anyone been successful with validating Biocare's HPV-16 and HPV
Cocktail Broad Spectrum (*both for IHC)* on either the Leica Bond System or
Ventana Bechmark XT?
Thanks,
-- 



Marian
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RE: [Histonet] Permanent Fluorescent Mounting Media

[Anatoli Gleiberman]

I tried it - got a free sample, liked how it looks (and especially price - 
two-times less than ProLong Gold)and ordered couple of bottles. Unfortunately 
they polymerized very fast and it was impossible to revive them. So, we 
switched back to ProLong.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Loralei Dewe
Sent: Tuesday, January 15, 2013 2:07 PM
To: Tina Van Meter
Cc: histonet@lists.utsouthwestern.edu; Suresch, Donna L.
Subject: Re: [Histonet] Permanent Fluorescent Mounting Media

Oh BTW they will send a free sample out for testing!

Loralei


On Tue, Jan 15, 2013 at 11:05 AM, Loralei Dewe  wrote:

> Try www.Valley-Scientific.com
>
> Loralei
>
>
> On Tue, Jan 15, 2013 at 10:51 AM, Tina Van Meter 
> wrote:
>
>> ProLong Gold (Invitrogen)
>>
>>
>>
>> Sent from my iPhone
>>
>> On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L."
>> 
>> wrote:
>>
>> > Hello Histonet,
>> > Anyone know of a non-aqueous permanent mounting media for use with
>> fluorescent IHC?
>> >
>> > Donna L. Suresch
>> > Senior Imaging Research Scientist
>> > Merck Research Laboratories
>> > Department of Imaging - West Point Campus
>> > Mail Stop:  WP44KOffice: WP44-H129
>> > 770 Sumneytown Pike
>> > PO Box 4
>> > West Point, PA  19486-0004
>> > Phone:  215-652-7349
>> > Fax:  215-993-6803
>> >
>> >
>> > Notice:  This e-mail message, together with any attachments,
>> > contains information of Merck & Co., Inc. (One Merck Drive,
>> > Whitehouse Station, New Jersey, USA 08889), and/or its affiliates
>> > Direct contact information for affiliates is available at
>> > http://www.merck.com/contact/contacts.html) that may be
>> > confidential, proprietary copyrighted and/or legally privileged. It
>> > is intended solely for the use of the individual or entity named on
>> > this message. If you
>> are
>> > not the intended recipient, and have received this message in
>> > error, please notify us immediately by reply e-mail and then delete
>> > it from your system.
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Re: [Histonet] Permanent Fluorescent Mounting Media

[Loralei Dewe]

Oh BTW they will send a free sample out for testing!

Loralei


On Tue, Jan 15, 2013 at 11:05 AM, Loralei Dewe  wrote:

> Try www.Valley-Scientific.com
>
> Loralei
>
>
> On Tue, Jan 15, 2013 at 10:51 AM, Tina Van Meter 
> wrote:
>
>> ProLong Gold (Invitrogen)
>>
>>
>>
>> Sent from my iPhone
>>
>> On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L." 
>> wrote:
>>
>> > Hello Histonet,
>> > Anyone know of a non-aqueous permanent mounting media for use with
>> fluorescent IHC?
>> >
>> > Donna L. Suresch
>> > Senior Imaging Research Scientist
>> > Merck Research Laboratories
>> > Department of Imaging - West Point Campus
>> > Mail Stop:  WP44KOffice: WP44-H129
>> > 770 Sumneytown Pike
>> > PO Box 4
>> > West Point, PA  19486-0004
>> > Phone:  215-652-7349
>> > Fax:  215-993-6803
>> >
>> >
>> > Notice:  This e-mail message, together with any attachments, contains
>> > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
>> > New Jersey, USA 08889), and/or its affiliates Direct contact information
>> > for affiliates is available at
>> > http://www.merck.com/contact/contacts.html) that may be confidential,
>> > proprietary copyrighted and/or legally privileged. It is intended solely
>> > for the use of the individual or entity named on this message. If you
>> are
>> > not the intended recipient, and have received this message in error,
>> > please notify us immediately by reply e-mail and then delete it from
>> > your system.
>> > ___
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Re: [Histonet] Permanent Fluorescent Mounting Media

[Loralei Dewe]

Try www.Valley-Scientific.com

Loralei


On Tue, Jan 15, 2013 at 10:51 AM, Tina Van Meter wrote:

> ProLong Gold (Invitrogen)
>
>
>
> Sent from my iPhone
>
> On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L." 
> wrote:
>
> > Hello Histonet,
> > Anyone know of a non-aqueous permanent mounting media for use with
> fluorescent IHC?
> >
> > Donna L. Suresch
> > Senior Imaging Research Scientist
> > Merck Research Laboratories
> > Department of Imaging - West Point Campus
> > Mail Stop:  WP44KOffice: WP44-H129
> > 770 Sumneytown Pike
> > PO Box 4
> > West Point, PA  19486-0004
> > Phone:  215-652-7349
> > Fax:  215-993-6803
> >
> >
> > Notice:  This e-mail message, together with any attachments, contains
> > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
> > New Jersey, USA 08889), and/or its affiliates Direct contact information
> > for affiliates is available at
> > http://www.merck.com/contact/contacts.html) that may be confidential,
> > proprietary copyrighted and/or legally privileged. It is intended solely
> > for the use of the individual or entity named on this message. If you are
> > not the intended recipient, and have received this message in error,
> > please notify us immediately by reply e-mail and then delete it from
> > your system.
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Re: [Histonet] Permanent Fluorescent Mounting Media

[Tina Van Meter]

ProLong Gold (Invitrogen)



Sent from my iPhone

On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L."  
wrote:

> Hello Histonet,
> Anyone know of a non-aqueous permanent mounting media for use with 
> fluorescent IHC?
> 
> Donna L. Suresch
> Senior Imaging Research Scientist
> Merck Research Laboratories
> Department of Imaging - West Point Campus
> Mail Stop:  WP44KOffice: WP44-H129
> 770 Sumneytown Pike
> PO Box 4
> West Point, PA  19486-0004
> Phone:  215-652-7349
> Fax:  215-993-6803
> 
> 
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
> New Jersey, USA 08889), and/or its affiliates Direct contact information
> for affiliates is available at 
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from 
> your system.
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Re: [Histonet] RE: Permanent Fluorescent Mounting Media

[Paula Sicurello]

James,

When I was at MCV we used Crystal Mount for our frozen IF's (skin,
kidney, etc.)  You put a few drops on, place it in the oven for a
little bit (I do not recall the temperture of the oven) and you had a
nice hard cover over your sections.

I hope this helps.

Paula
-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091




On Tue, Jan 15, 2013 at 1:25 PM, James Watson  wrote:
> Has anyone used hardmount mounting medias for fluorescence in a high volume 
> situation (up to 120 slides a day at times) when the slides are scanned?  We 
> have tested BioCares, Thermofishers, and Vectashields.  We found that we 
> liked the Thermo Immu-Mount best. Biocare Fluoro care antifade mountant 
> worked, but with scanning we saw some stains were out of focus.  The 
> VECTASHIELD HardSet Mounting Medium with DAPI gave us a higher percentage of 
> fading and out of focus images.
>
> Our issue with the hardmount and scanning is that we see one edge of the 
> slide in focus and the other out of focus frequently.  We are working on our 
> mounting technique to see if this can be corrected by modifying our 
> coverslipping technique.
>
> Any suggestions would be appreciated.  This does not make your fluorescence 
> permanent since most fluorochromes fade with age.
>
> James Watson HT  ASCP
> GNF  Genomics Institute of the Novartis Research Foundation
> Tel858-332-4647
> Fax   858-812-1915
> jwat...@gnf.org
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suresch, 
> Donna L.
> Sent: Tuesday, January 15, 2013 10:08 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Permanent Fluorescent Mounting Media
>
> Hello Histonet,
> Anyone know of a non-aqueous permanent mounting media for use with 
> fluorescent IHC?
>
> Donna L. Suresch
> Senior Imaging Research Scientist
> Merck Research Laboratories
> Department of Imaging - West Point Campus
> Mail Stop:  WP44KOffice: WP44-H129
> 770 Sumneytown Pike
> PO Box 4
> West Point, PA  19486-0004
> Phone:  215-652-7349
> Fax:  215-993-6803
>
>
> Notice:  This e-mail message, together with any attachments, contains 
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New 
> Jersey, USA 08889), and/or its affiliates Direct contact information for 
> affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential, 
> proprietary copyrighted and/or legally privileged. It is intended solely for 
> the use of the individual or entity named on this message. If you are not the 
> intended recipient, and have received this message in error, please notify us 
> immediately by reply e-mail and then delete it from your system.
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[Histonet] RE: Permanent Fluorescent Mounting Media

[James Watson]

Has anyone used hardmount mounting medias for fluorescence in a high volume 
situation (up to 120 slides a day at times) when the slides are scanned?  We 
have tested BioCares, Thermofishers, and Vectashields.  We found that we liked 
the Thermo Immu-Mount best. Biocare Fluoro care antifade mountant worked, but 
with scanning we saw some stains were out of focus.  The VECTASHIELD HardSet 
Mounting Medium with DAPI gave us a higher percentage of fading and out of 
focus images.  

Our issue with the hardmount and scanning is that we see one edge of the slide 
in focus and the other out of focus frequently.  We are working on our mounting 
technique to see if this can be corrected by modifying our coverslipping 
technique.

Any suggestions would be appreciated.  This does not make your fluorescence 
permanent since most fluorochromes fade with age.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Tel    858-332-4647
Fax   858-812-1915
jwat...@gnf.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna 
L.
Sent: Tuesday, January 15, 2013 10:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Permanent Fluorescent Mounting Media

Hello Histonet,
Anyone know of a non-aqueous permanent mounting media for use with fluorescent 
IHC?

Donna L. Suresch
Senior Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
Mail Stop:  WP44KOffice: WP44-H129
770 Sumneytown Pike
PO Box 4
West Point, PA  19486-0004
Phone:  215-652-7349
Fax:  215-993-6803


Notice:  This e-mail message, together with any attachments, contains 
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Jersey, USA 08889), and/or its affiliates Direct contact information for 
affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, 
proprietary copyrighted and/or legally privileged. It is intended solely for 
the use of the individual or entity named on this message. If you are not the 
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[Histonet] histosearch

[Elizabeth Cameron]

Anyone else having issues with histosearch today

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[Histonet] Permanent Fluorescent Mounting Media

[Suresch, Donna L.]

Hello Histonet,
Anyone know of a non-aqueous permanent mounting media for use with fluorescent 
IHC?

Donna L. Suresch
Senior Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
Mail Stop:  WP44KOffice: WP44-H129
770 Sumneytown Pike
PO Box 4
West Point, PA  19486-0004
Phone:  215-652-7349
Fax:  215-993-6803


Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
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please notify us immediately by reply e-mail and then delete it from 
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[Histonet] FW: [IHCRG] ms abs on rabbit tissue

[Patsy Ruegg]

  _  

From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of
Patsy Ruegg
Sent: Tuesday, January 15, 2013 9:49 AM
To: ih...@googlegroups.com
Subject: [IHCRG] ms abs on rabbit tissue

 

Does anyone have a mouse ab worked out on rabbit tissue?  We got a ms ab
from Abcam that is supposed react in Human, rat and rabbit but we are having
trouble getting it to stain the rabbit tissue.  Please advise.

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Fax: 720-859-4110

  pru...@ihctech.net

 

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[Histonet] Interstate Pathology and Consultations

[Pratt, Caroline]

 

I know this is a relatively hot topic and there are varying
interpretations of the law on the issue of Interstate Pathology and
Consultations.  I have read CAP's stance, but I was wondering if anyone
has any good articles, grids, charts, tables, etc. on interstate
pathology practices.  I am particularly looking for the states that do
not allow even consultations by out of state pathologists and details on
guidelines for interstate consultation for the more stringent states.
Thanks for any guidance you can provide.

 

Car J

 

Caroline M. Pratt, MBA

Practice Administrator Dermpath

3020 Market Street, Ste 201

Philadelphia, PA  19104

Phone 215-349-8178

Fax 215-662-6150

 



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RE: [Histonet] Independent Equipment Service Companies

[Rathborne, Toni]

Where are you located?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Tuesday, January 15, 2013 9:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Independent Equipment Service Companies

I lost the contact number I had for a company that will service histology 
equipment.
Could you please give me some contact info.

Thank You
Stella
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[Histonet] Independent Equipment Service Companies

[Stella Mireles]

I lost the contact number I had for a company that will service histology
equipment.
Could you please give me some contact info.

Thank You
Stella
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