[Histonet] (no subject)
http://www.blulobster.ca/tfvbbmcz.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unsubscribe
Joe Nocito, BS, PACM, HTCM (ASCP) QIHC Dept of Pathology/ 59LSQ/SGVLH Lackland AFB, TX 78236 joseph.noc...@us.af.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H&E Stainer Protocol
Hi Everyone, I was wondering if anyone out there is using a Sakura DRS-601 Diversified H&E Stainer. We are having trouble setting up an appropriate H&E staining protocol to have stains come out correctly. If anyone uses this instrument and is willing to share their protocol / times / solutions etc. it would be greatly appreciated. Thank you very much for your help! Stacy Giroux, HTL(ASCP) CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] re vacuoles in nuclei of PT Link treated tissue
The fact that the vacuoles no longer appear when you use manual dewaxing seem to point the problem at the DAKO product. I think that you will be better off consulting with DAKO because whatever we in HistoNet could tell you would be completely speculative and probably of little help, unless somebody having the same experience found the solution. René J. From: Steven Weston To: "histonet@lists.utsouthwestern.edu" Sent: Saturday, February 2, 2013 7:48 PM Subject: [Histonet] re vacuoles in nuclei of PT Link treated tissue Hi everyone, I have recently encountered an unusual result in our immunostaining. We have been bvery successfully using Dako's PT link with the High PH soln to dewax and heat retrieve some of our immuno samples. In conjunction with my colleagues from the local Hospital Anat Path dept we noticed a strange but prominent vacuolisation showing in the Nuclei (and less so in cytoplasm) in our treated tissue after staining with Haematoxylin. Here is the interesting thing, when we manually dewax the same type of sample and then through the high PH, PT link the vacuoles no longer appear. This was noticed in both laboratories. the first thought was that it had something to do with the wax. But we both use different waxes (precision cut and paraplast). Clearly it is something to do with the wax removal process but coming up with an explanation is challenging. Any ideas? Has anyone else noticed this phenomenon ? regards steve weston lab manager resp research group MRI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] congo red
Yes, smears should be fixed. I used NBF. René J. From: Nancy Schmitt To: "Histonet (histonet@lists.utsouthwestern.edu)" Sent: Sunday, February 3, 2013 7:48 AM Subject: [Histonet] congo red Hi to all- What is your process for pretreating congo red smears? Are you fixing them? We are running the stain on the Dako Artisan staining system. Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HER-2
That is a very strange question by your pathologists, since they are the ones doing the reporting. If they follow the reporting guidelines and arrive to a 2+ result, they should report it. Another question would be: are our lab's 2+ reports above the "mean" for other labs? The answer to this question can be done only if your find out the 2+ percentage for all labs is, and compare with yours. I think that your pathologists should contact CAP to find out if there are statistics on this issue. René J. From: Wilson A To: "histonet@lists.utsouthwestern.edu" Sent: Friday, February 1, 2013 9:52 PM Subject: [Histonet] HER-2 Hi, Our pathologists are concerned we may be reporting too many 2+ HER2’s. Can someone help with this? Thanks, Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Her-2 procedure
The protocol described by DAKO is very explicit, it produces consistent results and is the one I followed. Check it out. René J. From: Wilson A To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 3, 2013 12:20 AM Subject: [Histonet] Her-2 procedure Hi, Please I will appreciate it, if you guys could share your HER-2 PROCEDURE with me. Thanks, Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] congo red
Hi to all- What is your process for pretreating congo red smears? Are you fixing them? We are running the stain on the Dako Artisan staining system. Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
