Re: [Histonet] Cresyl echt Violett for Nissl substance

[Lee & Peggy Wenk]

Cresyl Echt Violet (CEV) is the pre-WWI name. After factories in Germany 
were bombed, the formulation for this stain was lost (that's the story I 
heard in the 70's).


Closest companies since then have made is cresyl violet acetate (CVA), 
though for many decades, continued to call it CEV. Only recently  (maybe 
last 10 years) have companies been calling it CVA, which is confusing to 
labs that need to order a 25 gram bottle once every 25 years.


Here's how we make it up:
CRESYL ECHT VIOLET
Cresyl echt violet (Cresyl Violet Acetate)0.5 g
Sodium acetate (CH3COONa3H2O)0.18 g
Distilled water500.0 mL
Acetic acid, concentrated (CH3COOH)1.5 mL (up to)
Dissolve cresyl echt violet and sodium acetate in distilled water. Slowly 
add acetic acid, drop by drop, to solution. Should have a pH of 3.5. If 
solution pH is below 3.5, add more sodium acetate. If solution pH is above 
3.5, add more acetic acid. Filter. Let stand overnight before using. Store 
at room temperature. Stable for months. May be reused until weak.


Stain for 1-2 hours. Differentiate in 2 changes 95% alcohol, until 
background cytoplasm is clear (check with microscope). Run up through 2 
changes 100% reagent alcohol, 2 changes xylene or substitute, coverslip. 
Nissl, RNA and DNA (nuclei) will be purple.


To speed up differentiation, and to pull more out of the DNA/nuclei, add 2 
drops of acetic acid conc. to the first 95% alcohol.


It might be CI # 51180, but I don't have that info at home.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
3601 W. 13 Mile Rd.
Royal Oak, MI 48073

Opinions expressed by me to not represent my place of employment.

-Original Message- 
From: Yaskovich, Ruth A (NIH/NIDCR) [E]

Sent: Thursday, May 02, 2013 3:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cresyl echt Violett for Nissl substance

What is everyone using for this stain?
Ruth Yaskovich
N.I.H.
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RE: [Histonet] H pylori and Ventana instruments

[Sebree Linda A]

Rae,

We use Ventana's HP antibody with no problems.

Linda A. Sebree

University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792

(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz
Sent: Tuesday, May 07, 2013 12:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H pylori and Ventana instruments

Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori 
antibody you are using, and whether or not you are experiencing non-specific 
staining, or staining that looks specific but with a stippled effect. 

Thanks

Rae Staskiewicz
UnityPoint Health-Methodist


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[Histonet] cap anp.23120

[anita dudley]

Wondering what people are doing about the new cap anp.23120?  validating tissue 
processing schedules.  Thanks a lot
 
Anita Dudley
Providence Hospital
Mobile, Alabama   
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[Histonet] histotech job opening, Merck Research Laboratories, Palo Alto CA

[Yearley, Jennifer Holmes]

Histotechnician/Histotechnologist - PHA001165
https://merck.taleo.net/careersection/merck_external_career_section/jobdetail.ftl?job=575074&lang=en
Merck is a global health care leader with a diversified portfolio of 
prescription medicines, vaccines and consumer health products, as well as 
animal health products.   Today, we are building a new kind of healthcare 
company - one that is ready to help create a healthier future for all of us.
Our ability to excel depends on the integrity, knowledge, imagination, skill, 
diversity and teamwork of people like you.  To this end, we strive to create an 
environment of mutual respect, encouragement and teamwork.  As part of our 
global team, you'll have the opportunity to collaborate with talented and 
dedicated colleagues while developing and expanding your career.

The Pathology Department at Merck Research Laboratories, Palo Alto, is 
currently seeking an energetic, self-motivated, highly collaborative 
histotechnician/histotechnologist  to join our histology group to help support 
basic research, mechanistic studies, and biomarker development for evaluations 
of candidate biological pharmaceuticals.  The successful candidate will have a 
strong histology background, excellent organizational skills and be a confident 
multitasker, able to juggle multiple responsibilities in a team environment, 
often under stringent time pressures. Responsibilities for the position will 
include:
*Assistance with mouse tissue collection/tissue handling at necropsy
*Familiarity with and implementation of routine fixation and 
decalcification protocols
*Grossing in of a wide array of human and animal tissues, both osseous 
and non-osseous
*Assistance with data entry and database updating for specimens in 
in-house tissue bank
*Fixed tissue processing and maintenance of tissue processors
*Tissue embedding into paraffin/OCT
*Sectioning of FFPE and frozen tissue blocks
*Labeling of slides and tissue cassettes using automated slide and 
cassette printers
*Performance of routine (H&E) and special histochemical stains (both 
manual and automated)

The succesful candidate will be a strong communicator with good writing skills, 
which will be used to generate well-organized, comprehensive, and timely 
documentation of work planned and results on completion. The successful 
candidate will occasionally be responsible, with supervision, for designing 
small experiments to test new methods, improve old methods, and/or answer 
well-defined questions pertaining to tissue properties. The successful 
candidate will consult with investigators on points of technical detail 
regarding tissue handling as needed. Comfort with common office computer 
software (e.g. Microsoft Office) is essential.
Education:
* BA/BS in the biological sciences, with a minimum of 3 years full-time 
work in a biological research or clinical laboratory or a MS degree in 
Biological Sciences
Preferred Skills and Experience:
* HT(ASCP) or HTL(ASCP) certification
* Familiarity with principles and techniques of immunohistochemistry

Our employees are the key to our company's success.  We demonstrate our 
commitment to our employees by offering a competitive and valuable rewards 
program.  Merck's benefits are designed to support the wide range of goals, 
needs and lifestyles of our employees, and many of the people that matter the 
most in their lives.

Search Firm Representatives Please Read Carefully:
Merck is not accepting unsolicited assistance from search firms for this 
employment opportunity.  Please, no phone calls or emails.  All resumes 
submitted by search firms to any employee at Merck via-email, the Internet or 
in any form and/or method without a valid written search agreement in place for 
this position will be deemed the sole property of Merck.  No fee will be paid 
in the event the candidate is hired by Merck as a result of the referral or 
through other means.



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[Histonet] Immunohistochmistry

[Michelle M. Mackinnon]

Has anyone tried or have a Wave RPD system from Celerus Diagnostics in their 
pathology lab? If yes, do you stain immunohistochemisrty on Mohs and paraffin 
embedded slides?  Would you suggest this instrument for a small lab?  Also what 
are the pros and cons using the Wave RPD system performing paraffin embedded 
tissue and mohs?



dermatology
CONSULTANTS
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[Histonet] H pylori and Ventana instruments

[Rae Staskiewicz]

Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori 
antibody you are using, and whether or not you are experiencing non-specific 
staining, or staining that looks specific but with a stippled effect. 

Thanks

Rae Staskiewicz
UnityPoint Health-Methodist


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RE: [Histonet] Monitoring Histology Quality

[Jesus Ellin]

We use Sunquest PowerPath to electronically show this information as well as 
keep records for data minning and Quality improvement

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna
Sent: Tuesday, May 07, 2013 10:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Monitoring Histology Quality

Is anyone using an electronic system to communicate histology quality info from 
the pathologists to Histology management? Also, do you use this same system to 
demonstrate to the CAP that histology quality is being checked daily, and if 
so, how do you indicate that slide quality was checked when there are no 
quality issues on a given day?
 
We operate in Sunquest CoPath.
 
Thank you,
 
Roger Heyna
Histology Supervisor
Maywood, IL

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Thank You.
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[Histonet] Monitoring Histology Quality

[Roger Heyna]

Is anyone using an electronic system to communicate histology quality info from 
the pathologists to Histology management? Also, do you use this same system to 
demonstrate to the CAP that histology quality is being checked daily, and if 
so, how do you indicate that slide quality was checked when there are no 
quality issues on a given day?
 
We operate in Sunquest CoPath.
 
Thank you,
 
Roger Heyna
Histology Supervisor
Maywood, IL
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[Histonet] Colorado Sakura Sales Rep

[Elizabeth Chlipala]

Does anyone out there have the contact information for the Sakura Rep for 
Colorado.   I have been trying to contact someone regarding an embedding cryo 
console but have not been successful.

I do have an additional question, our cryo console has essentially stopped 
working,  we only purchased it about a year and a half ago and we don't even us 
it every day, it started having problems several months ago.  One of my 
equipment repair guys told me that he has seen this before.   I'm just checking 
to see if this is a rare event or not.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
l...@premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


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FW: [Histonet] histo special request forms

[Vanessa Perez]

Ours is built into our LIS system. So when the doc places the order also puts 
the charges in.


Vanessa 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara 
Baldwin/mhhcc.org
Sent: Tuesday, May 07, 2013 9:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] histo special request forms

Hi Histonetters
Would anyone be willing to share their histo special request forms, we are 
thinking of using these to keep track of what our pathologists want done some 
write it down and some just call our histotechs and we are losing track of 
billing!!  So would someone please share?

Thanks
Histology/Cytology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 
0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 
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[Histonet] histo special request forms

[Sara Baldwin/mhhcc.org]

Hi Histonetters
Would anyone be willing to share their histo special request forms, we are 
thinking of using these to keep track of what our pathologists want done some 
write it down and some just call our histotechs and we are losing track of 
billing!!  So would someone please share?

Thanks
Histology/Cytology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-996-0210, 0216, Fax 812-996-0232, 
Pager 812-481-0897, Cell 812-887-3357
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