[Histonet] Recording formalin "out times"

[Wanda Shotsberger Gray]

Anyone out there using CoPath "paper free"? We are trying to use as little 
paper as possible, and have yet to come up with a good way/place to record the 
times that breast tissue comes out of formalin. If we use the "comment" line, 
the poor tech who scans the cassettes into CoPath must enter the time on every 
case, which can be way too many cases to make this feasible. We have also tried 
putting the out time as the name of the run, but the pathologists, who want 
this info in the report, can't easily see this information, and end up calling 
us for it.
Any help would be appreciated, and vendors: Are you listening? Make this part 
of your LIS  product, please.
Thank you all,
Wanda Shotsberger Gray
HT/HTL (ASCP)___
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Re: [Histonet] p40

[Akemi Allison]

Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting 
last weekend.
 
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com





 From: "Taylor, Jean" 
To: "'ih...@googlegroups.com'" ; 
"'histonet@lists.utsouthwestern.edu'"  
Sent: Friday, May 10, 2013 10:53 AM
Subject: [Histonet] p40
 

Hi everyone,

I'm looking into ordering the p40 antibody for one of our pathologists. He 
wants to use it to help distinguish between adenocarcinoma and squamous cell ca 
in lung. I'm wondering what labs are using, the monoclonal or polyclonal 
antibody, and where you purchase it from. I haven't found that a lot of 
companies that carry it. Any info would be helpful.

Thanks,

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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[Histonet] ground section staining

[Yan Liang]

Hi Histonet,
We are staining plastic bone ground section. However we have trouble on plastic 
ground section with H&E, Sandersons with acid fuchsin counterstaining and 
Goldner's Trichrome staining . It would be great appreciated if you can help  
or share protocol with us.

Thanks you
Yan
..
Yan Liang   Ph.D.
Senior Director, Histopathology

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RE: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen

[Elizabeth Chlipala]

Krista

Not sure if this would help but we mordant in Bouins for 1 hour at 60C, we let 
the bouins come up to temp (60C) before we put the slides in.  We have not had 
good success with temps lower than 60C and if we do not warm up the bouins.  We 
also find that fresh reagents are key in successful results, we make up our 
reagents, we don't use a kit.  If you don't need the alcian blue portion of the 
stain present, my suggestion would be to try a modified trichome, - mordant in 
bouins and in place of the iron hematoxylin use verhoff elastic stain, 
differenciate and then counterstain with a regular trichrome.  This will turn 
out really nice and it will demonstrates elastic fibers, collagen and muscle 
nicely.  We prefer doing this over the Movats.  I have a protocol if you need 
one.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
l...@premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Krista Sider
Sent: Friday, May 10, 2013 3:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid 
Fuchsin from Collagen

Hello All,

I have successfully stained porcine and mouse paraffin embedded heart tissues 
with Movat's Pentachrome (MP), using EMS' MP solutions and protocol 
(http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some 
optimization on times.

However, now I am working with archival human tissue that has been fixed for 
much longer and is much older than my other samples (Human Aorta & Aortic 
Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored 
at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin 
(WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% 
Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin's initial 
fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) 
and taking the PA or PM up to 1 hr, yet there is always a lot of red still left 
in the collagen, in some regions as strong as the muscle (which I am sure are 
not muscle or dense cells). I see virtually no change with increased PA or PM 
time. As I have aorta in my samples I can't just leave out the muscle stain.

I would be very grateful for your insights into anything I could try to get 
clean collagen and stained muscle in the MP stain. Why might my method not be 
working? Is there something I can substitute for the WSAF that might be 
appropriate?

Thank you very much for you help,

Krista
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[Histonet] Movat Pentachrome - Can’t Remove Woodstain Scarlet-Acid Fuchsin from Collagen

[Krista Sider]

Hello All,

I have successfully stained porcine and mouse paraffin embedded heart
tissues with Movat’s Pentachrome (MP), using EMS’ MP solutions and protocol
(http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with
some optimization on times.

However, now I am working with archival human tissue that has been fixed
for much longer and is much older than my other samples (Human Aorta &
Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin
and stored at RT for ~6 years). I cannot seem to get the Woodstain
Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5%
Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have
tried increasing the Bouin’s initial fixation (max 2 hrs 50*C), diluting
the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1
hr, yet there is always a lot of red still left in the collagen, in some
regions as strong as the muscle (which I am sure are not muscle or dense
cells). I see virtually no change with increased PA or PM time. As I have
aorta in my samples I can’t just leave out the muscle stain.

I would be very grateful for your insights into anything I could try to get
clean collagen and stained muscle in the MP stain. Why might my method not
be working? Is there something I can substitute for the WSAF that might be
appropriate?

Thank you very much for you help,

Krista
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[Histonet] Extra Ventana Reagent

[Delray Beach Pathology Kari Simeone]

Hi everyone. I use the Ventana NexES and when opening a new kit for PAS, I end 
up tossing the extra (2) Schiff's 2 (22ml each) bottles. Is there anyone out 
there that can use this stuff? I'd love to help someone instead of just wasting 
it. I inquired with Ventana and they basically told me that's just how they 
sell it. Yuck, wasteful. It's already registered so of no use on any other 
instrument but who knows??

Kari M Simeone
Histology/Iummunohistochemisrty Specialist Supervisor
Alternate Laboratory Supervisor
Leavitt Management Group
Advanced Dermatology & Cosmetic Surgery
561.819.0857 ext 100
561.819.6517 fax
ksime...@leavittmgt.com
www.advancedderm.com 

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the sender immediately by return e-mail and delete the original message.


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Re: [Histonet] GI biopsy H&E staining

[Jennifer MacDonald]

Use a Gill hematoxylin.

Sent from my iPad

On May 10, 2013, at 11:05 AM, "Annie T. Walton"  wrote:

> Hello all,
>
> Our Medical Director would like the goblet cells on our GI biopsies to
stain a darker blue. I hand stain using the following protocol:
>
>
>  *   Xylene, 3 changes
3 minutes each
>  *   100% Alcohol, 2 changes
1 minute each
>  *   95% Alcohol
1 minute
>  *   Tap water
rinse until water runs off evenly
>  *   Harris Hematoxylin
5 minutes
>  *   Water wash
rinse until water runs clear
>  *   Clarifier 2
5 seconds
>  *   Running water
30 seconds
>  *   Bluing Solution
1 minute
>  *   Tap water
rinse well
>  *   95% Alcohol
30 seconds
>  *   Eosin Y
1 minute
>  *   100% Alcohol, 2 changes
30 seconds
>  *   100% Alcohol, 2 changes
1 minute each
>  *   Xylene, 3 changes
1 minute each
>
> I have recently increased the hematoxylin time from 4 to 5 minutes and
decreased the time in clarifier from 25 to 5 seconds so the hematoxylin
doesn't get washed out as much, but it is not good enough. I also switched
from 70% alcohol to 95% before eosin and increased the time in eosin from
45 seconds to 1 minute to provide a greater contrast in hopes that that
would work, but the goblet cells are still not staining dark enough. Any
suggestions would greatly be appreciated!
>
> Thanks!
>
> Annie Walton, HTL (ASCP)
> Histology Technician
> Overlake Internal Medicine Associates Histology Lab
> 425-974-9643
> awal...@oima.org
>
>
> 
> DISCLAIMER: This message is confidential, intended only for the named
recipient(s) and may contain information that is privileged or exempt from
disclosure under applicable law. If you are not the intended recipient(s),
you are notified that the dissemination, distribution or copying of this
information is strictly prohibited. If you received this message in error,
please notify the sender then delete this message.
> ___
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[Histonet] GI biopsy H&E staining

[Annie T. Walton]

Hello all,

Our Medical Director would like the goblet cells on our GI biopsies to stain a 
darker blue. I hand stain using the following protocol:


 *   Xylene, 3 changes  
  3 minutes each
 *   100% Alcohol, 2 changes
   1 minute each
 *   95% Alcohol
1 minute
 *   Tap water  
  rinse until water runs off evenly
 *   Harris Hematoxylin 
 5 minutes
 *   Water wash 
   rinse until water runs clear
 *   Clarifier 2
 5 seconds
 *   Running water  
30 seconds
 *   Bluing Solution
  1 minute
 *   Tap water  
  rinse well
 *   95% Alcohol
30 seconds
 *   Eosin Y
  1 minute
 *   100% Alcohol, 2 changes
   30 seconds
 *   100% Alcohol, 2 changes
   1 minute each
 *   Xylene, 3 changes  
  1 minute each

I have recently increased the hematoxylin time from 4 to 5 minutes and 
decreased the time in clarifier from 25 to 5 seconds so the hematoxylin doesn't 
get washed out as much, but it is not good enough. I also switched from 70% 
alcohol to 95% before eosin and increased the time in eosin from 45 seconds to 
1 minute to provide a greater contrast in hopes that that would work, but the 
goblet cells are still not staining dark enough. Any suggestions would greatly 
be appreciated!

Thanks!

Annie Walton, HTL (ASCP)
Histology Technician
Overlake Internal Medicine Associates Histology Lab
425-974-9643
awal...@oima.org



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[Histonet] p40

[Taylor, Jean]

Hi everyone,

I'm looking into ordering the p40 antibody for one of our pathologists. He 
wants to use it to help distinguish between adenocarcinoma and squamous cell ca 
in lung. I'm wondering what labs are using, the monoclonal or polyclonal 
antibody, and where you purchase it from. I haven't found that a lot of 
companies that carry it. Any info would be helpful.

Thanks,

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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RE: [Histonet] Vantage and Prisma

[WILLIAM DESALVO]

I use Vantage and Sakura Prisma. The Sakura Prisma is not interfaced into 
Vantage. You place a bar-coded label on your H&E slides at microtomy. We scan 
the slides after staining, case assembly.

William DeSalvo, BS HTL(ASCP)
Production Manager-Anatomic Pathology
Chair, NSH Quality Management Committee
Owner/Consultant, Collaborative Advantage Consulting

 

> To: histonet@lists.utsouthwestern.edu
> From: jmasla...@stpetes.org
> Date: Fri, 10 May 2013 09:35:12 -0600
> Subject: [Histonet] Vantage and Prisma
> 
> Happy Friday
> Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma 
> stainer?
> 
> 
> Joe Maslanka BS, CT,HT (ASCP)
> Anatomical Pathology Technical Supervisor
> St Peter's Hospital,MT 59601
> (P)(406) 447-2406
> (F)(406)444-2126 
> 
> Give thanks for ALL things.
> "Kindness is the language the blind can see & the deaf can hear- Mark 
> Twain
> 
> 
> 
> This electronic mail message contains information which is confidential. 
> If you are not the intended recipient, please be aware that any 
> disclosure, photocopying, distribution or use of the contents of the 
> received information is prohibited. If you have received this e-mail in 
> error, please reply to the sender immediately and permanently delete this 
> message and all copies of it. Thank you.
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[Histonet] Full Time Openings for Histotech and Grossing Tech

[Laurie Colbert]

PATH MD has two full time openings for a histotech and a grossing tech.  Both 
positions require at least two years of experience and the ability to 
troubleshoot a variety of issues.  The histotechnician shift is from 11:00pm to 
7:30 am and entails embedding, cutting, and manual special stains.  The 
grossing tech shift is 10:30 am to 7:00 pm, Monday-Friday.

PATH MD is a new pathology reference lab located in West Hollywood, CA.  We are 
rapidly expanding and are looking to add motivated and experienced personnel to 
our team.  Salary is competitive and full medical, dental, and vision benefits 
are offered.  If interested, please forward your resume to: 
lcolb...@pathmdlabs.com


Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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[Histonet] Fibrin staining

[Jo-Ann Bader, Ms.]

We have a client who is interested in studying both fibrin loose in the mouse 
tissue as well as intravascular (arterial or venous).   It has been suggested I 
do either a Picro-Mallory or a Martius, Scarlet & Blue (MSB) stain.  Is there 
any other stains I could do, which is the most popular?  Where could I get 
either control blocks or slides.  I have been looking but have not come across 
any yet.

Thanks

Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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RE: [Histonet] Vantage and Prisma

[Morken, Timothy]

Joe, since the Prisma can't read bar codes you will need to label the slides 
before staining (usually at the microtome, which also acts as a checkpoint 
between cutting and staining) and then hand-scan them after they come out of 
the stainer. 


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jmasla...@stpetes.org
Sent: Friday, May 10, 2013 8:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Vantage and Prisma

Happy Friday
Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma 
stainer?


Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126 

Give thanks for ALL things.
"Kindness is the language the blind can see & the deaf can hear- Mark 
Twain



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[Histonet] Vantage and Prisma

[JMaslanka]

Happy Friday
Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma 
stainer?


Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126 

Give thanks for ALL things.
"Kindness is the language the blind can see & the deaf can hear- Mark 
Twain



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disclosure, photocopying, distribution or use of the contents of the 
received information is prohibited. If you have received this e-mail in 
error, please reply to the sender immediately and permanently delete this 
message and all copies of it. Thank you.
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[Histonet] RE: ANP.21382-Evaluating reagents lacking exp. date

[Morken, Timothy]

We label liquids with a 3-year expiration and solids with a 5 year expiration. 
I try to order amounts that we will use it up within that time period (tho 
usually a shorter time period for most things, like a year or less). If not, we 
run a test  on the reagent and relabel it for another 3 or 5 years.

I also asked that question to Fisher Scientific tech support and they sent me a 
letter stating that reagents without expiration dates were expected to last 
indefinitely under the stated storage conditions, but generally 3 to 5 years 
was a reasonable assumption for shelf life.

One thing you can do to ensure shelf life of powders is order hydrated 
versions. Anhydrous versions will absorb  water from the air over time and so 
have less shelf life.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Turner, Leandra
Sent: Friday, May 10, 2013 5:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ANP.21382-Evaluating reagents lacking exp. date

Hi Everyone,

This CAP question has probably been posted before, so I apologize in advance.
But I was wondering if anyone would be willing to share some ideas about the 
best way to handle this question.


Thank You,
Leandra Turner, HT (ASCP)cm
Dell Children's Medical Center




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[Histonet] ANP.21382-Evaluating reagents lacking exp. date

[Turner, Leandra]

Hi Everyone,

This CAP question has probably been posted before, so I apologize in advance.
But I was wondering if anyone would be willing to share some ideas about the 
best way to handle this question.


Thank You,
Leandra Turner, HT (ASCP)cm
Dell Children's Medical Center




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