[Histonet] Bone Marrow Issues

[Jordan Phillips]

HI everyone.  We are currently having problems with all of our bone marrows, 
mainly clots, washing off the slides.  We fix our bone marrows in Zenkers.  We 
use positive charged slides, put the slides in the oven for a minimum of 2 
hours and hand depar with no agitation.  When we get to the water, the sections 
just float off the slides, leaving just a rim of the tissue.   Any tips or 
suggestions would be greatly appreciated! 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue

[Krista Sider]

Hi Gudrun

How deep would you consider the oxidation to go? I have tried samples from
around 200um into the block. The Alkaline Alcohol converts the alcian blue
to monastral fast blue, which is insoluble - this is what i have been told.
So locks it in I guess. Doesn't really matter as I don't get alcian blue in
the sample to lock in.

Thanks
Krista
On May 28, 2013 10:09 AM, "Gudrun Lang"  wrote:

> Hi Krista,
> Maybe the bad stainability is more due to oxidation by air of the old
> block-surface than due to fixation. A deeper slide for comparison would
> show.
> I don't know what the alkaline alcohol is good for ?
>
> Gudrun
>
> -Ursprüngliche Nachricht-
> Von: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Krista
> Sider
> Gesendet: Montag, 27. Mai 2013 23:28
> An: histonet@lists.utsouthwestern.edu
> Betreff: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue
>
> Hi All,
>
> I had wonderful help from this site for my Woodstain Scarlet-Acid Fuchsin
> binding collagen problem. Now that this is fixed I have realized that the
> Alcian Blue is not staining my excessively fixed samples (Human Aorta &
> Aortic Valve, formalin fixed 2-4 years, embedded in paraffin and stored at
> RT for ~6 years). I have some samples fixed for only 2-6 months and these
> are fine but the samples fixed for 2-4 years do not uptake the Alcian Blue.
> I have taken it up to 1 hr in the 1% Alcian Blue solution followed by 10
> min
> in 56*C Alkaline Alcohol solution. I do a 1hr Bouin's 50*C to start (I have
> also tried Bouin's at 60*C).
>
> Any ideas on how I can get the Alcian Blue to stain these excessively fixed
> samples or possibly a substitute? I need to see the proteoglycans/GAGs.
>
> Thank you
> Krista
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] looking for an IHC tech

[Curt]

We're a southern CA lab looking for someone to help grow our IHC dept. as well 
as contribute with some routine bench work. I'm not going to go into great 
detail here, please contact if you're interested in exploring the opportunity.

PLEASE NO HEAD HUNTERS OR JOB PLACEMENT AGENTS.


Curt
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Temperature strips:

[Burton, Lynn]

That's what we do.
Lynn Burton
Animal Disease Lab
Galesburg, Il

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Tuesday, May 28, 2013 1:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature strips:

Does anyone know if we have to keep our temperature strips from our decloaking 
chamber/pressure cooker for CAP?  We have currently been stapling them to our 
log. 

Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Temperature strips:

[Jb]

Does anyone know if we have to keep our temperature strips from our decloaking 
chamber/pressure cooker for CAP?  We have currently been stapling them to our 
log. 

Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Checklist

[Luis Teran]

Does anyone have a checklist for Thermo Shannon Cryotome?
Sent from my iPhone

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: another Movats question (paraffin)

[Molinari, Betsy]

Donella,
I use Verhoffs.



-


Betsy  Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | www.texasheart.org

6770 Bertner Ave., MC 1-283, Houston, TX 77030



Confidentiality Notice: This message may be confidential and/or privileged. If 
you are not the intended recipient you may not review, disseminate or copy this 
e-mail, its contents and/or any attachments. Please immediately notify the 
sender If you have received this e-mail in error and delete it from your 
computer system.

-

-Original Message-
From: Donella Stillings [mailto:dstilli...@unipathdx.com]
Sent: Tuesday, May 28, 2013 10:56 AM
To: Molinari, Betsy
Subject: RE: another Movats question (paraffin)

Are you using Weigert's Hematoxylin or regular Hematoxylin?

Donella

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy
Sent: Tuesday, May 28, 2013 9:47 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] another Movats question (paraffin)

Hi,
The Alcian Blue in my Movats  is very faint or seems to completely disappear. I 
have been running Movats for years and this is a new problem. I have checked 
lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in 
solution for 20 mins, rinse for 5 mins in tap H2O,  then in alkaline alcohol 
(90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But 
after hematoxylin the blue is either gone or very faint.
Any suggestions?
Thanks,
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston , TX 77030
832-355-6524 (lab)
832-355-6812 (fax)







Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart Institute][THI 
News]
 [THI on Facebook]   [THI on 
Flicker]   [THI on Google] 
   [THI on Pinterest] 
  [THI on Twitter] 
  [THI on You Tube] 

Confidentiality Notice: This message may be confidential and/or privileged. If 
you are not the intended recipient you may not review, disseminate or copy this 
e-mail, its contents and/or any attachments. Please immediately notify the 
sender If you have received this e-mail in error and delete it from your 
computer system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Reagent containers for VIP5

[Diana McCaig]

Does anyone have spare reagent containers for a VIP5 they are willing to sell.  
I am looking for 3 to use exclusively for warm water wash.  We are located in 
Ontario, Canada

Diana

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Leica microtome, buying used vs. new?

[Kate Davoli]

Hi All,

I need to acquire a Leica RM2235 microtome.  I've got a quote from Leica
directly, and several quotes from refurbished / used ones too with
warranties for 6 months parts and labor.

So here's my question: *does anyone recommend strongly against getting
refurbished models?*  The list seems to appreciate Leica products in
general, partly because of how reliable the machinery is.  So are there
serious drawbacks to buying second hand?  Are there certain refurbishing
vendors that are more reliable than others?

Any advice would be greatly appreciated.

Thanks in advance,

Katherine Davoli
Supervisor, Tissue Culture & Histology Core
Department of Ophthalmology
University of Pittsburgh
Mail Stop Code: EEI010901
928 Eye & Ear Institute
203 Lothrop Street
Pittsburgh, PA 15213
(412) 647-8256
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] another Movats question (paraffin)

[koellingr]

Betsy, 
Don't know if applicable to this situation but in the past I have on occasion 
in other labs noted some stain going off and after checking everything, looked 
at the tap water. In fact I backtracked through the water utility once to see 
where the water came from, was delivered and what the utility did to purify. 
Convinced, although never proven, that tap water was culprit on at least one or 
two occasions. Utility would say they used "this chemical" to purify but on 
occasion changed to "that chemical". Although we fall into the habit that tap 
water is tap water it can change from time to time (source, purification, 
metals eroding in old pipes, etc). Don't have to be a chemist to drink tap 
water in one part of US and find it completely different tasting from another 
part of country. Maybe not likely cause but worth considering. 
Ray 
Research Scientist 
UW Seattle 

- Original Message -
From: "Betsy Molinari"  
To: Histonet@lists.utsouthwestern.edu 
Sent: Tuesday, May 28, 2013 8:47:19 AM 
Subject: [Histonet] another Movats question (paraffin) 

Hi, 
The Alcian Blue in my Movats is very faint or seems to completely disappear. I 
have been running Movats for years and this is a new problem. I have checked 
lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in 
solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 
ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after 
hematoxylin the blue is either gone or very faint. 
Any suggestions? 
Thanks, 
Betsy Molinari HT(ASCP) 
Texas Heart Institute 
Cardiovascular Pathology 
6770 Bertner Ave. 
Houston , TX 77030 
832-355-6524 (lab) 
832-355-6812 (fax) 



 



Betsy Molinari 
Senior Histology Research Technician 
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org 



6770 Bertner Ave., MC 1-283, Houston, TX 77030 



[Texas Heart Institute][THI 
News]
 [THI on Facebook]  [THI on 
Flicker]  [THI on Google] 
 [THI on Pinterest] 
 [THI on Twitter] 
 [THI on You Tube] 
 
Confidentiality Notice: This message may be confidential and/or privileged. If 
you are not the intended recipient you may not review, disseminate or copy this 
e-mail, its contents and/or any attachments. Please immediately notify the 
sender If you have received this e-mail in error and delete it from your 
computer system. 

___ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] another Movats question (paraffin)

[Molinari, Betsy]

Hi,
The Alcian Blue in my Movats  is very faint or seems to completely disappear. I 
have been running Movats for years and this is a new problem. I have checked 
lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in 
solution for 20 mins, rinse for 5 mins in tap H2O,  then in alkaline alcohol 
(90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But 
after hematoxylin the blue is either gone or very faint.
Any suggestions?
Thanks,
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston , TX 77030
832-355-6524 (lab)
832-355-6812 (fax)







Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart Institute][THI 
News]
 [THI on Facebook]   [THI on 
Flicker]   [THI on Google] 
   [THI on Pinterest] 
  [THI on Twitter] 
  [THI on You Tube] 

Confidentiality Notice: This message may be confidential and/or privileged. If 
you are not the intended recipient you may not review, disseminate or copy this 
e-mail, its contents and/or any attachments. Please immediately notify the 
sender If you have received this e-mail in error and delete it from your 
computer system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] antibodies online

[Connolly, Brett M]

I find that the individual antibody search engines I use each provide info from 
select companies so I generally look at several ...in addition to Biocompare, 
Google, Human Protein Atlas and PubMed searches.

http://www.antibodyresource.com/home

http://www.antibodydirectory.com/index2.php

http://www.labome.com/

http://www.linscottsdirectory.com/

http://www.nordiqc.org/Default.htm

and, here is one for working on murine tissue specifically, I don't think it 
gets updated though  

http://ncifrederick.cancer.gov/rtp/lasp/phl/immuno/

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803












-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, May 28, 2013 9:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antibodies online

Hello Histonetters!

What search engines do you use to find antibodies that are available
commercially? I use biocompare, but I'm not sure if that's the best site.
 I would just google it, but that brings up wy too many protocols and
papers (though the papers are sometimes a good source as well)
Searching individual companies sometimes helps, but I've found that
anti-chick antibodies are usually sold by small companies.

Emily


"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue

[Gudrun Lang]

Hi Krista,
Maybe the bad stainability is more due to oxidation by air of the old
block-surface than due to fixation. A deeper slide for comparison would
show.
I don't know what the alkaline alcohol is good for ?

Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Krista
Sider
Gesendet: Montag, 27. Mai 2013 23:28
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue

Hi All,

I had wonderful help from this site for my Woodstain Scarlet-Acid Fuchsin
binding collagen problem. Now that this is fixed I have realized that the
Alcian Blue is not staining my excessively fixed samples (Human Aorta &
Aortic Valve, formalin fixed 2-4 years, embedded in paraffin and stored at
RT for ~6 years). I have some samples fixed for only 2-6 months and these
are fine but the samples fixed for 2-4 years do not uptake the Alcian Blue.
I have taken it up to 1 hr in the 1% Alcian Blue solution followed by 10 min
in 56*C Alkaline Alcohol solution. I do a 1hr Bouin's 50*C to start (I have
also tried Bouin's at 60*C).

Any ideas on how I can get the Alcian Blue to stain these excessively fixed
samples or possibly a substitute? I need to see the proteoglycans/GAGs.

Thank you
Krista
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] antibodies online

[Elizabeth Chlipala]

Emily

We use biocompare, google, pub med and human protein atlas.  I always try to 
look at some papers if I can, with papers you can review materials and methods 
and also review images, etc.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
l...@premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, May 28, 2013 7:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antibodies online

Hello Histonetters!

What search engines do you use to find antibodies that are available 
commercially? I use biocompare, but I'm not sure if that's the best site.
 I would just google it, but that brings up wy too many protocols and 
papers (though the papers are sometimes a good source as well) Searching 
individual companies sometimes helps, but I've found that anti-chick antibodies 
are usually sold by small companies.

Emily


"By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward."

-Chuck Palahniuk, "Haunted"
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] antibodies online

[Emily Sours]

Hello Histonetters!

What search engines do you use to find antibodies that are available
commercially? I use biocompare, but I'm not sure if that's the best site.
 I would just google it, but that brings up wy too many protocols and
papers (though the papers are sometimes a good source as well)
Searching individual companies sometimes helps, but I've found that
anti-chick antibodies are usually sold by small companies.

Emily


"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet