RE: [Histonet] Shandon Excelsior Tissue Processor

[Tony Henwood (SCHN)]

Vanessa,

We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector.

Cost under $1,500 Australian.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa
Sent: Thursday, 13 June 2013 2:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Shandon Excelsior Tissue Processor

Does any one having problems with the Excelsior Tissue Processor power board 
repeatedly after power outage?  This has happened to me three times in the last 
five years even though it has backup batteries.
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[Histonet] Paraffin processing native sheep ACL

[Elizabeth Ronan]

Hello,

I need to paraffin process native sheep anterior cruciate ligament (ACL) 
that has been fixed in 10% neutral buffered formalin for 7 days. I was 
wondering if anyone with more expertise on this subject could guide me 
with the best lengths of times in the alcohols, xylenes, and paraffin to 
fix ACL. I tried a 12 hour program but the sections crumbled in the 
middle and it appeared that the paraffin had not fully perfused the 
ligament.


I have access to the following program, and can alter the lengths of the 
steps for as long as desired:


Program:
70%
80%
95%
95%
100%
100%
Xylene
Xylene
Paraffin
Paraffin
Paraffin

Any advice is much appreciated.
Thanks for your time,
Liz


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[Histonet] Histology Openings

[Anna Nolan]

Hi my name is Anna and I work with Prometheus Healthcare doing laboratory
recruitment. Currently I'm looking to fill several histology positions.
Please call or email me if any of these positions are of interest to you.

 

 

Portland, ME

 -Histology Technologist (3AM-11AM)

 

NYC

 -Grossing Specialist (3rd shift)

 -Histology Tech (1st shift) 

 

Suffern, NY

 -IHC Histology Tech (1st shift)

 -Cutting embedding, staining, and coverslipping. IHC Experience a plus.
(7AM-4PM)

 

Anna Nolan
Recruiter
Prometheus Healthcare
Office 301-693-9057
Cell 301-693-8908
Fax 301-368-2478
  anolan
 @prometheushealthcare.com
 
http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6
 
  www.prometheushealthcare.com

 

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[Histonet] Tissue Processors

[Matthew Roark]

So in the next couple of months we are getting ready to demo Thermo's STP
420ES  and Leica's ASP6025 tissue processor . 
 
Looking for the good, bad, and ugly about them.   So if you have one  or
have had a demo please share what you've found.  
 
Thanks!!
 
 
 
Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
  mro...@sfmc.net
  http://www.sfmc.net
 
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[Histonet] Shandon Excelsior Tissue Processor

[Velez, Vanessa]

Does any one having problems with the Excelsior Tissue Processor power
board repeatedly after power outage?  This has happened to me three times
in the last five years even though it has backup batteries.
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[Histonet] RE: Control Storage

[Helen Fedor]

Hello, We are storing our unstained slides at -20 in Ziploc bags. Our TMA 
blocks are being stored at 4 degrees.

Helen L. Fedor 

Oncology Tissue Services, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065

410.614.1660

http://tmalab.jhmi.edu/
http://prostatebiorepository.org/



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Wednesday, June 12, 2013 11:56 AM
To: Herring, Colleen; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Control Storage

We store our blocks at room temp, our IHC slides are stored in the fridge.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen 
[colleen_herr...@bshsi.org]
Sent: Wednesday, June 12, 2013 9:48 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control Storage

Does anyone have any ideas or suggestions about the storage of blocks and 
precut slides for immunos?


The information in this communication is intended to be confidential to the 
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is 
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the 
intended recipient, do not copy or use the information contained within this 
communication, or allow it to be read, copied or utilized in any manner, by any 
other person(s). Should this communication be received in error, please notify 
the sender immediately either by response e-mail or by phone, and permanently 
delete the original e-mail, attachment(s), and any copies.

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[Histonet] RE: Control Storage

[Elizabeth Chlipala]

We store our blocks at room temp, our IHC slides are stored in the fridge.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen 
[colleen_herr...@bshsi.org]
Sent: Wednesday, June 12, 2013 9:48 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control Storage

Does anyone have any ideas or suggestions about the storage of blocks and 
precut slides for immunos?


The information in this communication is intended to be confidential to the 
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is 
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the 
intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized 
in any manner, by any other person(s). Should
this communication be received in error, please notify the sender immediately 
either by response e-mail or by phone,
and permanently delete the original e-mail, attachment(s), and any copies.

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[Histonet] Control Storage

[Herring, Colleen]

Does anyone have any ideas or suggestions about the storage of blocks and 
precut slides for immunos?


The information in this communication is intended to be confidential to the 
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is 
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the 
intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized 
in any manner, by any other person(s). Should
this communication be received in error, please notify the sender immediately 
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and permanently delete the original e-mail, attachment(s), and any copies.

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[Histonet] RE: Her-2 antibody

[Sue Hunter]

We have contacted Leica several times, had the machine calibrated/aligned many 
times, and that is what we have been told.  It seems to hold true as with only 
one label we have no problems.  Also, after the upgrade, we saw the problem a 
lot when we only ran one or two slides on a tray.  The magic number seems to be 
4 slides to get acceptable staining, but Leica suggests we load it up.   We had 
never had a problem until the upgrade in software.
Sue

-Original Message-
From: Houston, Ronald [mailto:ronald.hous...@nationwidechildrens.org] 
Sent: Wednesday, June 12, 2013 10:59 AM
To: Sue Hunter; Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu
Subject: RE: Her-2 antibody

Never had any such problems with either the Max or the Bond IIIs in the 6+ 
years we have had them(no matter how many labels are on the slide - we have had 
three labels on a slide with no problems at all), unless of course your tissue 
extends to the rim of the covertile - then what would you expect?
You certainly do not need to have all 10 spaces filled on the tray. 
Suggest you contact Leica service as it seems as though your machine needs 
calibrated and realigned

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Wednesday, June 12, 2013 10:42 AM
To: Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Her-2 antibody

We have a Bond Max and run mainly ER/PR and ISH stains on that.  After Leica 
loaded in a new version of software in October we started having problems like 
that too.  Seems you have to have all 10 spots on the tray filled with a slide 
- doesn't have to be programed, just a slide and cover tile for the weight.  We 
also were doing a workaround with a Vantage label under the Leica label and 
found out that the two label thickness interferes with the movement of the 
cover tile (sometimes...but not always), which was affecting our staining.  
Even a regular slide label under the Leica label will cause a problem.  We 
finally have gone to entering a case, printing the labels but not putting them 
on the slides, cutting the sections and then putting the labels on the slide 
for positive patient identification.  Not ideal, and I worry a lot about case 
mix ups, but that is what is working to get consistent staining here.  As they 
say, The Enemy of Good is Better.  Wish Leica had not made it better!  It was 
working very nicely before.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Fauck 
[CCDHB]
Sent: Tuesday, June 11, 2013 1:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Her-2 antibody

Hi All
 
We here in wellington Hospital New Zealand having some problem with the
Her-2 ( Lieca, NCL-L-CBE-356 )
 
Top half of the slide ( control or Test tissue) are strongly positive but if we 
put the control tissue on the bottom half it is week to negative!
 
Does any one has the same problems with your Her-2, it is done on a Leica Bond 
(Max) III.
 
Much appreciated for any suggestions, also from Leica Technical specialists.
 
Regards,
Robert Fauck
Wellington hospital, NZ


This email or attachment(s) may contain confidential or legally privileged 
information intended for the sole use of the addressee(s). Any use, 
redistribution, disclosure, or reproduction of this message, except as 
intended, is prohibited. If you received this email in error, please notify the 
sender and remove all copies of the message, including any attachments. Any 
views or opinions expressed in this email (unless otherwise stated) may not 
represent those of Capital & Coast District Health Board.

http://www.ccdhb.org.nz

(1C_S1)
 
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[Histonet] RE: Waterbaths

[Cheri Miller]

I agree with you. Ours seem to short out as well. I now only purchase the baths 
that have a separate Pyrex glass insert. They also provide more of an "edge" 
space to lay out your ribbons when cutting multiple levels and ribbons. Cheri

"March 10, 2013 is Histotechnology Professionals Day"
Cheryl A. Miller HT(ASCP)cm
Histology Supervisor,
Hygiene Officer
Physicians Laboratory Services
4840 F Street
Omaha, NE. 68127-0999
402 731 4145 ext. 554


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy
Sent: Wednesday, June 12, 2013 9:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Waterbaths

What are the best water bath to use?  I am using the Boekel water baths
and they always seem to become rusty and we have to purchase new ones.
Any suggestions?



LeAnn Murphy

Aultman Hospital

Canton, Ohio



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message in error, please notify the sender immediately and delete this email 
from your system.


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[Histonet] RE: Her-2 antibody

[Houston, Ronald]

Never had any such problems with either the Max or the Bond IIIs in the 6+ 
years we have had them(no matter how many labels are on the slide - we have had 
three labels on a slide with no problems at all), unless of course your tissue 
extends to the rim of the covertile - then what would you expect?
You certainly do not need to have all 10 spaces filled on the tray. 
Suggest you contact Leica service as it seems as though your machine needs 
calibrated and realigned

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Wednesday, June 12, 2013 10:42 AM
To: Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Her-2 antibody

We have a Bond Max and run mainly ER/PR and ISH stains on that.  After Leica 
loaded in a new version of software in October we started having problems like 
that too.  Seems you have to have all 10 spots on the tray filled with a slide 
- doesn't have to be programed, just a slide and cover tile for the weight.  We 
also were doing a workaround with a Vantage label under the Leica label and 
found out that the two label thickness interferes with the movement of the 
cover tile (sometimes...but not always), which was affecting our staining.  
Even a regular slide label under the Leica label will cause a problem.  We 
finally have gone to entering a case, printing the labels but not putting them 
on the slides, cutting the sections and then putting the labels on the slide 
for positive patient identification.  Not ideal, and I worry a lot about case 
mix ups, but that is what is working to get consistent staining here.  As they 
say, The Enemy of Good is Better.  Wish Leica had not made it better!  It was 
working very nicely before.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Fauck 
[CCDHB]
Sent: Tuesday, June 11, 2013 1:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Her-2 antibody

Hi All
 
We here in wellington Hospital New Zealand having some problem with the
Her-2 ( Lieca, NCL-L-CBE-356 )
 
Top half of the slide ( control or Test tissue) are strongly positive but if we 
put the control tissue on the bottom half it is week to negative!
 
Does any one has the same problems with your Her-2, it is done on a Leica Bond 
(Max) III.
 
Much appreciated for any suggestions, also from Leica Technical specialists.
 
Regards,
Robert Fauck
Wellington hospital, NZ


This email or attachment(s) may contain confidential or legally privileged 
information intended for the sole use of the addressee(s). Any use, 
redistribution, disclosure, or reproduction of this message, except as 
intended, is prohibited. If you received this email in error, please notify the 
sender and remove all copies of the message, including any attachments. Any 
views or opinions expressed in this email (unless otherwise stated) may not 
represent those of Capital & Coast District Health Board.

http://www.ccdhb.org.nz

(1C_S1)
 
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[Histonet] Waterbaths

[Leann M. Murphy]

What are the best water bath to use?  I am using the Boekel water baths
and they always seem to become rusty and we have to purchase new ones.
Any suggestions?

 

LeAnn Murphy

Aultman Hospital

Canton, Ohio

 

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[Histonet] RE: Her-2 antibody

[Sue Hunter]

We have a Bond Max and run mainly ER/PR and ISH stains on that.  After Leica 
loaded in a new version of software in October we started having problems like 
that too.  Seems you have to have all 10 spots on the tray filled with a slide 
- doesn't have to be programed, just a slide and cover tile for the weight.  We 
also were doing a workaround with a Vantage label under the Leica label and 
found out that the two label thickness interferes with the movement of the 
cover tile (sometimes...but not always), which was affecting our staining.  
Even a regular slide label under the Leica label will cause a problem.  We 
finally have gone to entering a case, printing the labels but not putting them 
on the slides, cutting the sections and then putting the labels on the slide 
for positive patient identification.  Not ideal, and I worry a lot about case 
mix ups, but that is what is working to get consistent staining here.  As they 
say, The Enemy of Good is Better.  Wish Leica had not made it better!  It was 
working very nicely before.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Fauck 
[CCDHB]
Sent: Tuesday, June 11, 2013 1:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Her-2 antibody

Hi All
 
We here in wellington Hospital New Zealand having some problem with the
Her-2 ( Lieca, NCL-L-CBE-356 )
 
Top half of the slide ( control or Test tissue) are strongly positive but if we 
put the control tissue on the bottom half it is week to negative!
 
Does any one has the same problems with your Her-2, it is done on a Leica Bond 
(Max) III.
 
Much appreciated for any suggestions, also from Leica Technical specialists.
 
Regards,
Robert Fauck
Wellington hospital, NZ


This email or attachment(s) may contain confidential or legally privileged 
information intended for the sole use of the addressee(s). Any use, 
redistribution, disclosure, or reproduction of this message, except as 
intended, is prohibited. If you received this email in error, please notify the 
sender and remove all copies of the message, including any attachments. Any 
views or opinions expressed in this email (unless otherwise stated) may not 
represent those of Capital & Coast District Health Board.

http://www.ccdhb.org.nz

(1C_S1)
 
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RE: [Histonet] Telepathology

[Ian R Bernard]

We are  a test site for the Aperio System.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp
Sent: Tuesday, June 11, 2013 9:09 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Telepathology

Does anyone have any recommendations for scanning equipment  to have slides 
read off site from main lab.  Thanks.

 

 

Gloria Tharp, BA, HTL(ASCP)

Director of Operations

PCA Southeast

gth...@pcasoutheast.com

931-490-1005

800-388-1294

 

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