[Histonet] Unsubscribe, Chapter 195
Hi, I do not disagree at all. in fact, I think it would make for an interesting management tool. If you have the nicest CV around, but a simple Histonet archive search for your name + "unsubscribe" shows that you can't follow simple instructions, it says something about your communication skills and raises interesting questions about weather you are a good employment candidate. One might also look for the various incarnations of misspelled attempts at unsubscribing such as "unscribe" and "unsiscribe". Just sayin' Amos On Fri, Sep 6, 2013 at 1:00 PM, wrote: > Message: 6 > Date: Fri, 6 Sep 2013 01:56:37 + (UTC) > From: nmhi...@comcast.net > Subject: [Histonet] Unsubscribe, Chapter 195 > To: HISTONET > Message-ID: > < > 1200426006.1029812.1378432597832.javamail.r...@sz0075a.emeryville.ca.mail.comcast.net > > > > Content-Type: text/plain; charset=utf-8 > > It is a concern that members of our technically-oriented career field >  have a difficult time understanding the method for unsubscribing to > Histonet. There is an  almost- daily posting to "unsubscribe", despite > the fact that this subject has been addressed literally hundreds of > times. When one "joins" Histonet, instructions are provided, should be > printed out for reference and used if the subscriber decides to leave the > group. We are required to be knowledgeable on all manner of technical > routines requiring detailed instructions and Histonet is no less clear in > the methods for joining and "un-joining". Use them, please. Fire away - > I'm retired and I can take the flak! I do miss my microtome, though... > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] FISH enumeration
Pathologists do FISH reading. Learning by doing. Manual enumeration. Each doctor, who orders a FISH has to read it by him/herself. Often they do it with a second person/second look for reliable results. Gudrun Lang Histolab, Linz, Austria -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von joelle weaver Gesendet: Samstag, 07. September 2013 16:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH enumeration For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? Thanks for any input. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tmcne...@lmhealth.org > To: thisis...@aol.com; histonet@lists.utsouthwestern.edu > Date: Fri, 6 Sep 2013 05:45:41 -0400 > Subject: RE: [Histonet] Cell Block Preparation > CC: > > This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. > - Spin in a conical tube and pour off > - Melt an agar slant (we get TSA slant from micro) > - Pour the agar into the conical tube and spin for 5 minutes > - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone > - The agar will slide out of the centrifuge tube > - Slice off the very tip and wrap in lens paper > - Place the wrapped tip in a cassette and process as usual > - Embed the specimen tip down and you are good to go... > > I still use this method today when I feel it necessary. Works great. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcne...@lmhealth.org > www.LMHealth.org > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. > > Does anyone have a more current technique which renders better cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FISH enumeration
For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? Thanks for any input. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tmcne...@lmhealth.org > To: thisis...@aol.com; histonet@lists.utsouthwestern.edu > Date: Fri, 6 Sep 2013 05:45:41 -0400 > Subject: RE: [Histonet] Cell Block Preparation > CC: > > This is how we do it now. In the old days, we used agar and to my mind, it > is still the best way when you have scant material. > - Spin in a conical tube and pour off > - Melt an agar slant (we get TSA slant from micro) > - Pour the agar into the conical tube and spin for 5 minutes > - The agar will re-solidify and whatever sediment there is will be > concentrated in the very tip of the cone > - The agar will slide out of the centrifuge tube > - Slice off the very tip and wrap in lens paper > - Place the wrapped tip in a cassette and process as usual > - Embed the specimen tip down and you are good to go... > > I still use this method today when I feel it necessary. Works great. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcne...@lmhealth.org > www.LMHealth.org > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We > currently spin, pour off the supernatant, retrieve the sediment and process > in lens paper. > > Does anyone have a more current technique which renders better cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, a > specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. If > you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you have > received this in error, please advise the sender by reply e-mail and delete > the message immediately. You may also contact the LMH Process Improvement > Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be > secure or error-free as information could be intercepted, corrupted, lost, > destroyed, arrive late or incomplete, or contain viruses. The sender > therefore does not accept liability for any errors or omissions in the > contents of this message, which arise as a result of e-mail transmission. > Thank you. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
