[Histonet] Goldner's Trichrome staining for Ground sectioned bone samples

[Edward Ren]

Dear all,

I have trouble in getting our bone tissues embedded in Technovit Resin stained 
(without deplasticization), especially the Goldner's Trichrome stain.

The details of our samples are: sheep tibias and pig fibulas embedded in 
Technovit 9100 New MMA resin, cut and ground with the Exakt cutting and 
grinding system to thickness of 10-50 µm. We followed the standard protocol for 
sledge microtome sectioned specimen and had some success with the Goldner's 
staining on these samples but later on this year it just stopped working. I 
tried protocols for ground sectioned bone tissues but had no success. It seems 
the stains have really poor penetration into the resin. I have also tried Von 
Kossa staining but also with poor results.

Has anyone had the same issue?

Hopefully I can get some expert guide here to solve these problems.

Any help will be appreciated.

Kind regards,
Edward Ren

PhD student at Queensland University of Tech
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Re: RE: [Histonet] Oil red O

[E. Wayne Johnson 朱稳森]

While you're at it, maybe some one can explain what the
-wah- in "diddy-wah-diddy" means?



On 3:59, Morken, Timothy wrote:

Just like "Formalin," a brand name now used interchangeably with "formaldehyde."

And your trivia of the day:

"Formaldehyde was the first polyatomic organic molecule detected in the interstellar 
medium"

Courtesy of Wikipedia.

Tim Morken
UCSF Pathology

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Monday, September 09, 2013 2:00 PM
To: Rene J Buesa; Ada Feldman; histonet
Subject: RE: [Histonet] Oil red O

I believe the answer should be - Oil Red O is a brand name (not uncommon in the 
dye indutry) and represents the two dyes found in the compound, Solvent Red 27 
and Sudan Red 5B

William DeSalvo, BS HTL(ASCP)

   

Date: Mon, 9 Sep 2013 13:32:08 -0700
From: rjbu...@yahoo.com
To: adafeld...@anatechltdusa.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil red O
CC:

There you have it!
René J.



From: Ada Feldman
To: histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 4:13 PM
Subject: Re: [Histonet] Oil red O


As you are finding out the answers to dye nomenclature you can had these endings to 
your list of the "O' in Oil red O:
Oil red EGN
Oil red 4B


Ada Feldman
Anatech Ltd.
1020 Harts Lake Road
Battle Creek, MI 49037

Phone: 800.262.8324
Phone: 269.964.6450
Fax: 269.964.8084
adafeld...@anatechltdusa.com




On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:

 

I'm not sure, but whatever you do... don't store it next to the Sudan Black 
B...you'll stink up the lab!!

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck&  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E.
Visser
Sent: Monday, September 09, 2013 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oil red O

Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser

Histotech Bronovo The Netherlands





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Re: [Histonet] Travel Histology Technician Jobs

[Jay Lundgren]

 I honestly wouldn't recommend it unless you are financially able to
undergo 6 month plus periods of unemployment.  In 2008 I didn't work all
year, only maybe the last 3 weeks.  The wages have never come back to the
pre- 2008 levels. The largest staffing agency in the country declared
bankruptcy because of the downturn.
  In today's economy, there is always going to be an unemployed person
who is willing to work for a couple of dollars less, and this affects the
agencies as well.  Most of the HR depts I've dealt with lately, ONLY care
about cost. As long as the applicant is JCAHO compliant and registered,
they'll take the one who is 50 cents cheaper.
  So, right now, the agencies are able to get people for ridiculously
low wages.  I won't be as crass as to post any, but they are basically
asking you to subsidize your own working vacation.  Usually in a
semi-rural, isolated, or climatically blasted location (there's a reason
they can't get a full time tech), living in the cheapest motel in town for
3 months.  When you ask the agency why they put you in that particular
motel, they reply, "Because it's the cheapest place in town. (DUH)"  Some
agencies are better than others, but they are all SUPER cheap.  The better
ones are just honest about it.
  I see full time jobs advertised regionally (Texas) that are offering
registered techs  jobs, right out of school,  $4-5 more than what the
agencies will offer you just starting out as a traveler, regardless of
previous experience.  Also, travel = NO BENEFITS.  Some of the agencies
pretend to have benefits, but as I said before, it's near impossible to
work with enough continuity to remain covered.
 Also, big warning:  A lot of the "agencies" aren't really agencies at
all, but someone with an 800 number and a website and some hold music.
  It ain't all speedboats and supermodels, if that's what you had in
mind.

  Sincerely,

  Jay A. Lundgren, M.S., HTL (ASCP)


On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen  wrote:

> Thanks, this is very timely as I'm looking into temp/travel HT.
> Any suggestions where to look, which agencies?
>  Thanks, Jeff Lowen  HT/MLT(ASCP)
>
> > Date: Mon, 9 Sep 2013 17:52:34 -0500
> > From: jaylundg...@gmail.com
> > To: lpw...@sbcglobal.net
> > Subject: Re: [Histonet] Travel Histology Technician Jobs
> > CC: histonet@lists.utsouthwestern.edu; cjac...@clinpath.com
>
> >
> > I've been traveling for the past 16 years! Not everyone who wanders is
> > lost.
> >
> > Sincerely,
> >
> > Jay A. Lundgren,
> > M.S., HTL (ASCP)
> >
> >
> >
> >
> > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk  >wrote:
> >
> > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in
> > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on
> > > "Work and Play Across the USA - A Guide to Being a Traveling Tech".
> > > http://www.histoconvention.**org/ 
>
> > > Click on Schedule
> > >
> > > Peggy A. Wenk, HTL(ASCP)SLS
> > >
> > > -Original Message- From: Christopher Jacobs
> > > Sent: Monday, September 09, 2013 1:30 PM
> > > To: 'histonet@lists.**utsouthwestern.edu<
> histonet@lists.utsouthwestern.edu>
>
> > > '
> > > Subject: [Histonet] Travel Histology Technician Jobs
> > >
> > >
> > > Histonetters,
> > >
> > > Does anyone out there have any information on traveling histology
> > > technician jobs? What is the compensation? How does insurance work?
> What
> > > qualifications are needed? I am particularly interested in any personal
> > > experiences any one has had.
> > >
> > > Thanks!
> > >
> > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP)
> > > Clin-Path Diagnostics, LLC
> > >
> > >
> > >
> > > CONFIDENTIALITY NOTICE: This message and any attachments are for the
> sole
> > > use of the intended recipient(s). This message is confidential and may
> also
> > > be privileged. If you are not the intended recipient, please contact
> the
> > > sender immediately, delete the contents of this message and do not use
> it
> > > for any purpose.
> > >
> > > __**_
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> > >
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
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> > >
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RE: [Histonet] Unregistered HT

[joelle weaver]

All I have is a "histology assistant" description I put together. It is mostly 
clerical, instrument up keep and other duties. My employer does not hire 
uncertified techs- due to CLIA license, grossing, FISH & molecular duties ( 
high complexity). I hope to be a clinical site eventually, and then they will 
have to complete their certification within one year -if they are hired on. 
If you think that will help, I can send it on.




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> Date: Mon, 9 Sep 2013 15:39:12 -0500
> From: dels...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Unregistered HT
> 
> Hi all,
> 
> I'm wondering if anyone can share a job description for an unregistered HT
> position. I also need to know if unregistered HT's are allowed to cut
> frozen sections.  I know there is a limit to what they can do, especially
> where IHC and special staining are concerned, but I really don't know about
> frozens, since it's a lot like regular microtomy, except for the H&E
> staining of the slide. I also understand that facilities might have
> different descriptions, but what I'm really looking for is what CAP allows
> them to do.
> 
> Thanks for your help,
> 
> Deloris Carter, HT(ASCP)
> SMMC
> Shawnee, KS
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Re: [Histonet] Travel Histology Technician Jobs

[Jay Lundgren]

I've been traveling for the past 16 years!  Not everyone who wanders is
lost.

   Sincerely,

 Jay A. Lundgren,
M.S., HTL (ASCP)




On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk wrote:

> On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in
> Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on
> "Work and Play Across the USA - A Guide to Being a Traveling Tech".
> http://www.histoconvention.**org/ 
> Click on Schedule
>
> Peggy A. Wenk, HTL(ASCP)SLS
>
> -Original Message- From: Christopher Jacobs
> Sent: Monday, September 09, 2013 1:30 PM
> To: 'histonet@lists.**utsouthwestern.edu
> '
> Subject: [Histonet] Travel Histology Technician Jobs
>
>
> Histonetters,
>
> Does anyone out there have any information on traveling histology
> technician jobs? What is the compensation? How does insurance work? What
> qualifications are needed? I am particularly interested in any personal
> experiences any one has had.
>
> Thanks!
>
> "CJ" Christopher P. Jacobs, HT QIHC(ASCP)
> Clin-Path Diagnostics, LLC
>
>
>
> CONFIDENTIALITY NOTICE: This message and any attachments are for the sole
> use of the intended recipient(s). This message is confidential and may also
> be privileged. If you are not the intended recipient, please contact the
> sender immediately, delete the contents of this message and do not use it
> for any purpose.
>
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RE: [Histonet] Travel Histology Technician Jobs

[jeff lowen]

Thanks, this is very timely as I'm looking into temp/travel HT.Any suggestions 
where to look, which agencies? Thanks, Jeff Lowen  
HT/MLT(ASCP)

> Date: Mon, 9 Sep 2013 17:52:34 -0500
> From: jaylundg...@gmail.com
> To: lpw...@sbcglobal.net
> Subject: Re: [Histonet] Travel Histology Technician Jobs
> CC: histonet@lists.utsouthwestern.edu; cjac...@clinpath.com
> 
> I've been traveling for the past 16 years!  Not everyone who wanders is
> lost.
> 
>Sincerely,
> 
>  Jay A. Lundgren,
> M.S., HTL (ASCP)
> 
> 
> 
> 
> On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk wrote:
> 
> > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in
> > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on
> > "Work and Play Across the USA - A Guide to Being a Traveling Tech".
> > http://www.histoconvention.**org/ 
> > Click on Schedule
> >
> > Peggy A. Wenk, HTL(ASCP)SLS
> >
> > -Original Message- From: Christopher Jacobs
> > Sent: Monday, September 09, 2013 1:30 PM
> > To: 'histonet@lists.**utsouthwestern.edu
> > '
> > Subject: [Histonet] Travel Histology Technician Jobs
> >
> >
> > Histonetters,
> >
> > Does anyone out there have any information on traveling histology
> > technician jobs? What is the compensation? How does insurance work? What
> > qualifications are needed? I am particularly interested in any personal
> > experiences any one has had.
> >
> > Thanks!
> >
> > "CJ" Christopher P. Jacobs, HT QIHC(ASCP)
> > Clin-Path Diagnostics, LLC
> >
> >
> >
> > CONFIDENTIALITY NOTICE: This message and any attachments are for the sole
> > use of the intended recipient(s). This message is confidential and may also
> > be privileged. If you are not the intended recipient, please contact the
> > sender immediately, delete the contents of this message and do not use it
> > for any purpose.
> >
> > __**_
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> > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet
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Re: [Histonet] Oil red O

[Rene J Buesa]

Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser 
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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Re: [Histonet] Oil red O

[Jennifer MacDonald]

I believe the question is what is the "O" signify.
as in OG6 the O is for orange.



From:   Rene J Buesa 
To: "P.E. Visser" , "histonet@lists.utsouthwestern.edu" 

Date:   09/09/2013 12:46 PM
Subject:Re: [Histonet] Oil red O
Sent by:histonet-boun...@lists.utsouthwestern.edu



Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser 
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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RE: [Histonet] Oil red O

[Mitchell Jean A]

I believe the question is:  what does the "O" in Oil Red O stand for?


Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital & Clinics
Neuromuscular Laboratory 
600 Highland Avenue
Madison, WI  53792-5132 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, September 09, 2013 2:45 PM
To: P.E. Visser; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil red O

Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO René J.



From: P.E. Visser 
To: histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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[Histonet] Unregistered HT

[Deloris Carter]

Hi all,

I'm wondering if anyone can share a job description for an unregistered HT
position. I also need to know if unregistered HT's are allowed to cut
frozen sections.  I know there is a limit to what they can do, especially
where IHC and special staining are concerned, but I really don't know about
frozens, since it's a lot like regular microtomy, except for the H&E
staining of the slide. I also understand that facilities might have
different descriptions, but what I'm really looking for is what CAP allows
them to do.

Thanks for your help,

Deloris Carter, HT(ASCP)
SMMC
Shawnee, KS
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Re: [Histonet] Oil red O

[Rene J Buesa]

Not sure because for that designation to be used there have to be 2 equal 
groups and Oil Red O has 4 benzene rings and in 2 of them there are 4 methyl 
groups in "para" position so I doubt that the "O" is for "ortho".
rené J.



From: "Goins, Tresa" 
To: "Grantham, Andrea L - (algranth)"  
Cc: HISTONET  
Sent: Monday, September 9, 2013 4:11 PM
Subject: RE: [Histonet] Oil red O


May be the position of the active group on the benzene ring - "ortho" vs. 
"meta" vs. "para" ?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Monday, September 09, 2013 2:06 PM
Cc: HISTONET
Subject: Re: [Histonet] Oil red O

Rene,
You still have not answered the question - what does the "O" stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415    Fax: 520.626.2097





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RE: [Histonet] needing nerves resin embedded

[Garcia, Lori, M.Sc.]

Also APS:

Jason Thorsten (HT, ASCP)Director, Pathology Services
American Preclinical Services, LLC
8945 Evergreen Boulevard
Minneapolis, Minnesota 55433
direct office-(763)951-8034
direct fax-(763)951-8035
mobile-(612)910-3390
jthors...@apsemail.com

www.americanpreclinical.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza
Sent: Monday, September 09, 2013 2:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] needing nerves resin embedded

Hi all:

My lab is starting a project involving neuromas. We are in need of resin 
embedding of the nerves, but our facility does not have the capabilities. Is 
there a company that offers resin embedding/sectioning services?

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[Histonet] needing nerves resin embedded

[Nicole Cosenza]

Hi all:

My lab is starting a project involving neuromas. We are in need of resin 
embedding of the nerves, but our facility does not have the 
capabilities. Is there a company that offers resin embedding/sectioning 
services?


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RE: [Histonet] needing nerves resin embedded

[Garcia, Lori, M.Sc.]

Wax-it  in Canada should be able to do this. Here is the contact information:

Contact: Aruna Somasiri
202 - 2386 East Mall
Vancouver, B.C., V6T 1Z3
Phone: 604-822-1595
i...@waxitinc.com
www.waxitinc.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza
Sent: Monday, September 09, 2013 2:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] needing nerves resin embedded

Hi all:

My lab is starting a project involving neuromas. We are in need of resin 
embedding of the nerves, but our facility does not have the capabilities. Is 
there a company that offers resin embedding/sectioning services?

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RE: [Histonet] Oil red O

[Morken, Timothy]

Just like "Formalin," a brand name now used interchangeably with "formaldehyde."

And your trivia of the day:

"Formaldehyde was the first polyatomic organic molecule detected in the 
interstellar medium"

Courtesy of Wikipedia.

Tim Morken
UCSF Pathology

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Monday, September 09, 2013 2:00 PM
To: Rene J Buesa; Ada Feldman; histonet
Subject: RE: [Histonet] Oil red O

I believe the answer should be - Oil Red O is a brand name (not uncommon in the 
dye indutry) and represents the two dyes found in the compound, Solvent Red 27 
and Sudan Red 5B

William DeSalvo, BS HTL(ASCP)

> Date: Mon, 9 Sep 2013 13:32:08 -0700
> From: rjbu...@yahoo.com
> To: adafeld...@anatechltdusa.com; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Oil red O
> CC: 
> 
> There you have it!
> René J.
> 
> 
> 
> From: Ada Feldman 
> To: histonet@lists.utsouthwestern.edu
> Sent: Monday, September 9, 2013 4:13 PM
> Subject: Re: [Histonet] Oil red O
> 
> 
> As you are finding out the answers to dye nomenclature you can had these 
> endings to your list of the "O' in Oil red O:
> Oil red EGN
> Oil red 4B
> 
> 
> Ada Feldman
> Anatech Ltd.
> 1020 Harts Lake Road
> Battle Creek, MI 49037
> 
> Phone: 800.262.8324
> Phone: 269.964.6450
> Fax: 269.964.8084
> adafeld...@anatechltdusa.com
> 
> 
> 
> 
> On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:
> 
> > I'm not sure, but whatever you do... don't store it next to the Sudan Black 
> > B...you'll stink up the lab!!
> > 
> > Brett M. Connolly, Ph.D.
> > Principal Scientist, Imaging Dept.
> > Merck & Co., Inc.
> > PO Box 4, WP-44K
> > West Point, PA 19486
> > brett_conno...@merck.com
> > T- 215-652-2501
> > F- 215-993-6803
> > 
> > 
> > 
> > 
> > 
> > 
> > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu 
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. 
> > Visser
> > Sent: Monday, September 09, 2013 3:41 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Oil red O
> > 
> > Hi all
> > 
> > I was requested where the O stands for. who has any suggestion.
> > 
> > 
> > 
> > Regards Piet Visser
> > 
> > Histotech Bronovo The Netherlands
> > 
> > 
> > 
> > 
> > 
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > Notice:  This e-mail message, together with any attachments, 
> > contains information of Merck & Co., Inc. (One Merck Drive, 
> > Whitehouse Station, New Jersey, USA 08889), and/or its affiliates 
> > Direct contact information for affiliates is available at
> > http://www.merck.com/contact/contacts.html) that may be 
> > confidential, proprietary copyrighted and/or legally privileged. It 
> > is intended solely for the use of the individual or entity named on 
> > this message. If you are not the intended recipient, and have 
> > received this message in error, please notify us immediately by 
> > reply e-mail and then delete it from your system.
> > 
> > 
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RE: [Histonet] Oil red O

[WILLIAM DESALVO]

I believe the answer should be - Oil Red O is a brand name (not uncommon in the 
dye indutry) and represents the two dyes found in the compound, Solvent Red 27 
and Sudan Red 5B

William DeSalvo, BS HTL(ASCP)

> Date: Mon, 9 Sep 2013 13:32:08 -0700
> From: rjbu...@yahoo.com
> To: adafeld...@anatechltdusa.com; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Oil red O
> CC: 
> 
> There you have it!
> René J.
> 
> 
> 
> From: Ada Feldman 
> To: histonet@lists.utsouthwestern.edu 
> Sent: Monday, September 9, 2013 4:13 PM
> Subject: Re: [Histonet] Oil red O
> 
> 
> As you are finding out the answers to dye nomenclature you can had these 
> endings to your list of the "O' in Oil red O:
> Oil red EGN
> Oil red 4B
> 
> 
> Ada Feldman
> Anatech Ltd.
> 1020 Harts Lake Road
> Battle Creek, MI 49037
> 
> Phone: 800.262.8324
> Phone: 269.964.6450
> Fax: 269.964.8084
> adafeld...@anatechltdusa.com
> 
> 
> 
> 
> On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:
> 
> > I'm not sure, but whatever you do... don't store it next to the Sudan Black 
> > B...you'll stink up the lab!!
> > 
> > Brett M. Connolly, Ph.D.
> > Principal Scientist, Imaging Dept.
> > Merck & Co., Inc.
> > PO Box 4, WP-44K
> > West Point, PA 19486
> > brett_conno...@merck.com
> > T- 215-652-2501
> > F- 215-993-6803
> > 
> > 
> > 
> > 
> > 
> > 
> > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu 
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
> > Sent: Monday, September 09, 2013 3:41 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Oil red O
> > 
> > Hi all
> > 
> > I was requested where the O stands for. who has any suggestion.
> > 
> > 
> > 
> > Regards Piet Visser 
> > 
> > Histotech Bronovo The Netherlands
> > 
> > 
> > 
> > 
> > 
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > Notice:  This e-mail message, together with any attachments, contains
> > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
> > New Jersey, USA 08889), and/or its affiliates Direct contact information
> > for affiliates is available at 
> > http://www.merck.com/contact/contacts.html) that may be confidential,
> > proprietary copyrighted and/or legally privileged. It is intended solely
> > for the use of the individual or entity named on this message. If you are
> > not the intended recipient, and have received this message in error,
> > please notify us immediately by reply e-mail and then delete it from 
> > your system.
> > 
> > 
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Re: [Histonet] Unregistered HT

[Pam Marcum]

Please if there is an exact list of duties let us all know.  

  

We have had trainees and we basically taught embedding, processing and 
staining, in other words basic Histology.  This included special stains as the 
persons involved would be taking their registries after one year of training.  
We do not gross in Histology so that was not an issue.  We did not allow 
cutting biopsies or in the end embedding them.  We trained them on the care and 
feeding of the instrumentation and what it was used for in Histology.  
Transplant biopsies and other more complicated types of tissue were still 
handled by someone with an HT or HTL only.  

  

They were listed during this time as Lab Aids and that was all.  We are no 
longer training people as it has it ups and downs that can affect the whole lab 
structure over time. 

  

Pam Marcum 

UAMS 




- Original Message -
From: "Deloris Carter"  
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:39:12 PM 
Subject: [Histonet] Unregistered HT 

Hi all, 

I'm wondering if anyone can share a job description for an unregistered HT 
position. I also need to know if unregistered HT's are allowed to cut 
frozen sections.  I know there is a limit to what they can do, especially 
where IHC and special staining are concerned, but I really don't know about 
frozens, since it's a lot like regular microtomy, except for the H&E 
staining of the slide. I also understand that facilities might have 
different descriptions, but what I'm really looking for is what CAP allows 
them to do. 

Thanks for your help, 

Deloris Carter, HT(ASCP) 
SMMC 
Shawnee, KS 
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Re: [Histonet] Oil red O

[Rene J Buesa]

There you have it!
René J.



From: Ada Feldman 
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 4:13 PM
Subject: Re: [Histonet] Oil red O


As you are finding out the answers to dye nomenclature you can had these 
endings to your list of the "O' in Oil red O:
Oil red EGN
Oil red 4B


Ada Feldman
Anatech Ltd.
1020 Harts Lake Road
Battle Creek, MI 49037

Phone: 800.262.8324
Phone: 269.964.6450
Fax: 269.964.8084
adafeld...@anatechltdusa.com




On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:

> I'm not sure, but whatever you do... don't store it next to the Sudan Black 
> B...you'll stink up the lab!!
> 
> Brett M. Connolly, Ph.D.
> Principal Scientist, Imaging Dept.
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_conno...@merck.com
> T- 215-652-2501
> F- 215-993-6803
> 
> 
> 
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
> Sent: Monday, September 09, 2013 3:41 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Oil red O
> 
> Hi all
> 
> I was requested where the O stands for. who has any suggestion.
> 
> 
> 
> Regards Piet Visser 
> 
> Histotech Bronovo The Netherlands
> 
> 
> 
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
> New Jersey, USA 08889), and/or its affiliates Direct contact information
> for affiliates is available at 
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from 
> your system.
> 
> 
> ___
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Re: [Histonet] Oil red O

[Rene J Buesa]

It is for Sudan Red 5B. All these "apothecary" names are essentially meaningless
René J.



From: Jennifer MacDonald 
To: 
Cc: "histonet@lists.utsouthwestern.edu" ; 
histonet-boun...@lists.utsouthwestern.edu; P.E. Visser  
Sent: Monday, September 9, 2013 3:53 PM
Subject: Re: [Histonet] Oil red O


I believe the question is what is the "O" signify.
as in OG6 the O is for orange.



From:  Rene J Buesa 
To:    "P.E. Visser" , "histonet@lists.utsouthwestern.edu" 

Date:  09/09/2013 12:46 PM
Subject:        Re: [Histonet] Oil red O
Sent by:        histonet-boun...@lists.utsouthwestern.edu



Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO
René J.



From: P.E. Visser 
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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RE: [Histonet] Oil red O

[Connolly, Brett M]

I'm not sure, but whatever you do... don't store it next to the Sudan Black 
B...you'll stink up the lab!!

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
Sent: Monday, September 09, 2013 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oil red O

Hi all

I was requested where the O stands for. who has any suggestion.

 

Regards Piet Visser 

Histotech Bronovo The Netherlands

 

 

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Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


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Re: [Histonet] Oil red O

[Rene J Buesa]

If that is the case, ORO will stain fat droplets in Frozen Sections. In FFPE 
will stain nothing.
René J.



From: Mitchell Jean A 
To: 'Rene J Buesa' ; P.E. Visser ; 
"histonet@lists.utsouthwestern.edu"  
Sent: Monday, September 9, 2013 3:49 PM
Subject: RE: [Histonet] Oil red O


I believe the question is:  what does the "O" in Oil Red O stand for?


Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital & Clinics
Neuromuscular Laboratory 
600 Highland Avenue
Madison, WI  53792-5132 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, September 09, 2013 2:45 PM
To: P.E. Visser; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil red O

Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that 
stain with ORO René J.



From: P.E. Visser 
To: histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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[Histonet] Oil red O

[P.E. Visser]

Hi all

I was requested where the O stands for. who has any suggestion.

 

Regards Piet Visser 

Histotech Bronovo The Netherlands

 

 

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[Histonet] Alizarin red/Alcian blue on sections

[Rui TAHARA]

Does anybody have protocol for Alizarin
red/Alcian blue (also counterstain with something else?) on sections. All I can
find is either stain or double staining on whole mounts. 


 

Thank you in advance, 


Rui TAHARA

PhD student
McGill University

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Re: [Histonet] Oil red O

[Rene J Buesa]

I wrote that apothecary names are meaningless = they do not have to have a 
specific meaning that, in this case, I do not have the foggiest idea! It does 
not have to be anything specific. 
Something is for sure: it has nothing to do with "orange".
 René J.



From: "Grantham, Andrea L - (algranth)" 
To: 
Cc: HISTONET  
Sent: Monday, September 9, 2013 4:06 PM
Subject: Re: [Histonet] Oil red O


Rene,
You still have not answered the question - what does the "O" stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415    Fax: 520.626.2097





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[Histonet] IHC Manager

[Anna Nolan]

I'm currently recruiting for an IHC Manager for a facility in southern San
Francisco. ASCP Certification is required. Feel free to contact me directly
with any questions. 

 

Anna Nolan
Recruiter
Prometheus Healthcare
Direct Line 301-693-8908
Office 301-693-9057
Fax 301-368-2478
  anolan
 @prometheushealthcare.com
 
http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6
 
  www.prometheushealthcare.com

 

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Re: [Histonet] Oil red O

[Ada Feldman]

As you are finding out the answers to dye nomenclature you can had these 
endings to your list of the "O' in Oil red O:
Oil red EGN
Oil red 4B


Ada Feldman
Anatech Ltd.
1020 Harts Lake Road
Battle Creek, MI 49037

Phone: 800.262.8324
Phone: 269.964.6450
Fax: 269.964.8084
adafeld...@anatechltdusa.com




On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote:

> I'm not sure, but whatever you do... don't store it next to the Sudan Black 
> B...you'll stink up the lab!!
> 
> Brett M. Connolly, Ph.D.
> Principal Scientist, Imaging Dept.
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_conno...@merck.com
> T- 215-652-2501
> F- 215-993-6803
> 
> 
> 
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
> Sent: Monday, September 09, 2013 3:41 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Oil red O
> 
> Hi all
> 
> I was requested where the O stands for. who has any suggestion.
> 
> 
> 
> Regards Piet Visser 
> 
> Histotech Bronovo The Netherlands
> 
> 
> 
> 
> 
> ___
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
> New Jersey, USA 08889), and/or its affiliates Direct contact information
> for affiliates is available at 
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from 
> your system.
> 
> 
> ___
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RE: [Histonet] Oil red O

[Goins, Tresa]

May be the position of the active group on the benzene ring - "ortho" vs. 
"meta" vs. "para" ?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Monday, September 09, 2013 2:06 PM
Cc: HISTONET
Subject: Re: [Histonet] Oil red O

Rene,
You still have not answered the question - what does the "O" stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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Re: [Histonet] Oil red O

[Grantham, Andrea L - (algranth)]

Rene,
You still have not answered the question - what does the "O" stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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[Histonet] VIP antibody

[Leila Etemadi]

Hello every body,

I wounded if any of you is working with VIP antibody ( rabbit)?, Any advice for 
selecting right supplier will be 

appreciated. The best of this antibody used to be presented by Eurodiagnostica, 
which unfortunately they are not 

presenting it any more for IHC project ( they just sell the kit now a days). 
Santa cruz, milipore….?!?, Any 

suggestion?!?


Many thanks,

Leila :-)
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Re: [Histonet] (no subject)

[Jennifer MacDonald]

Recommended melting point of paraffin is 2-4 degrees above the melting 
point of the paraffin.  Because we really don't see paraffins that would 
have a melting point of 46, the BEST answer would be 58-70.  Perhaps not 
what we do, but the best answer for the choices provided.



From:   "Martin, Erin" 
To: histonet 
Cc: "Naujokas, Agne" , "Meier, Melissa" 

Date:   09/09/2013 06:30 AM
Subject:[Histonet] (no subject)
Sent by:histonet-boun...@lists.utsouthwestern.edu



Good morning all!

One of our fellows emailed me a question that she came across while 
studying for her boards:



"I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting 
point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but 
we have also used paraffin that melts at 56C and we embedded at 58C.  Or 
am I missing something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

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[Histonet] Travel Histology Technician Jobs

[Christopher Jacobs]

Histonetters,

Does anyone out there have any information on traveling histology technician 
jobs? What is the compensation? How does insurance work? What qualifications 
are needed? I am particularly interested in any personal experiences any one 
has had.

Thanks!

"CJ" Christopher P. Jacobs, HT QIHC(ASCP)
Clin-Path Diagnostics, LLC



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RE: [Histonet] FISH enumeration

[Sue Hunter]

My techs do the scoring for the FISH testing.  We have a Leica Ariol system - 
the HER2 FISH are computer assisted, but the Urovysions are manually assessed.  
That may change with the new systems we are receiving.  My techs are 
exceptionally well trained in morphology - they sit with our director to learn, 
and are really really good. We do about 10 urovysions per week and sometimes as 
many as 30 pathvysions per week.  I have one or two techs on the FISH rotation 
each week.  The other techs step in as needed.  The pathologist circles the 
area on the HER2 IHC slide to be FISHed.  The Pathologists do come down to  the 
lab to see cases, but they rely on the techs.  The pathologists also look at 
all other criteria before signing out the case to make sure everything fits. We 
are also getting a new image hub so the saved images will be viewable by the 
pathologists - this will be a really nice addition to the reviewing process. I 
believe there are many laboratories where the cytotechs read out the urovsyions.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Saturday, September 07, 2013 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FISH enumeration

 For any laboratories out there who perform in house FISH procedures, if you 
could share what personnel are responsible for doing the signal enumeration & 
scoring? It would be helpful if you could describe the personnel's training and 
certification,  as well as an approximation of FTE's needed with some volumes. 
Do you do manual enumeration or use scanning software? 
Thanks for any input.





Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: tmcne...@lmhealth.org
> To: thisis...@aol.com; histonet@lists.utsouthwestern.edu
> Date: Fri, 6 Sep 2013 05:45:41 -0400
> Subject: RE: [Histonet] Cell Block Preparation
> CC: 
> 
> This is how we do it now.  In the old days, we used agar and to my mind, it 
> is still the best way when you have scant material.
> - Spin in a conical tube and pour off
> - Melt an agar slant (we get TSA slant from micro)
> - Pour the agar into the conical tube and spin for 5 minutes
> - The agar will re-solidify and whatever sediment there is will be 
> concentrated in the very tip of the cone
> - The agar will slide out of the centrifuge tube
> - Slice off the very tip and wrap in lens paper
> - Place the wrapped tip in a cassette and process as usual
> - Embed the specimen tip down and you are good to go...
> 
> I still use this method today when I feel it necessary.  Works great.
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcne...@lmhealth.org
> www.LMHealth.org
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
> Sent: Thursday, September 05, 2013 12:45 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Cell Block Preparation
> 
> 
> I am getting complaints in regard to "insufficient" cell blocks.  We 
> currently spin, pour off the supernatant, retrieve the sediment and process 
> in lens paper.
> 
> Does anyone have a more current technique which renders better cellularity?
> 
> Also, do you know which renders a better cell block:  a fresh specimen, a 
> specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
> 
> Thanks,
> Ann
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> therefore does not accept liability for any errors or omissions in the 
> contents of this message, which arise as a result of e-mail transmission. 
> Thank you.
> 
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[Histonet] unsubscribe

[Silverman, Jeffrey]

Jeffrey S. Silverman HT HTL QIHC (ASCP)
Operations Manager
Anatomic Pathology Specimen Processing,
Laboratory Safety Officer

Lenox Hill Hospital
12 Uris- Anatomic Pathology
100 East 77th Street
New York, New York 10075

office- 212 434 6673
cell-631 375 4505
email:  jsilver...@nshs.edu






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Monday, September 09, 2013 1:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 118, Issue 10

Send Histonet mailing list submissions to
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To subscribe or unsubscribe via the World Wide Web, visit
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than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: Cell Block Preparation (Cartun, Richard)
   2. Re: Unsubscribe, Chapter 195 (Emily Sours)
   3. (no subject) (Martin, Erin)
   4. RE: (no subject) (Manfre, Philip)
   5. RE: (no subject) (Marcum, Pamela A)
   6. RELIA Hot Histology Job Alert Histology Supervisorneeded in
  North Florida. (Pam Barker)
   7. Re: Unsubscribe, Chapter 195 (Lee & Peggy Wenk)
   8. Re: Unsubscribe, Chapter 195 (nmhi...@comcast.net)
   9. ICC on previously Wright stained smears (Susan Foreman)
  10. Re: ICC on previously Wright stained smears (Rene J Buesa)
  11. Re: Unsubscribe, Chapter 195 (dusko trajkovic)
  12. Re: (no subject) (Jennifer MacDonald)
  13. RE: FISH enumeration (Sue Hunter)


--

Message: 1
Date: Mon, 9 Sep 2013 00:20:12 +
From: "Cartun, Richard" 
Subject: RE: [Histonet] Cell Block Preparation
To: Ann Specian ,
"histonet@lists.utsouthwestern.edu"

Message-ID:
<9215bd4b0ba1b44d962a71c758b68d2e01882...@hhcexchmb05.hhcsystem.org>
Content-Type: text/plain; charset="us-ascii"

We borrowed a wonderful cell block procedure from Windham Community Memorial 
Hospital here in CT several years ago.  The specimen is collected fresh in 
sterile saline or RPMI and then centrifuged to concentrate the cells.  Plasma 
and then thrombin are added to form a clot.  The clot is then fixed in formalin 
and processed in our Histology Laboratory.  I will send the procedure to anyone 
who is interested.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.car...@hhchealth.org


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Ann Specian 
[thisis...@aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation

I am getting complaints in regard to "insufficient" cell blocks.  We currently 
spin, pour off the supernatant, retrieve the sediment and process in lens paper.

Does anyone have a more current technique which renders better cellularity?

Also, do you know which renders a better cell block:  a fresh specimen, a 
specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann
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--

Message: 2
Date: Mon, 9 Sep 2013 07:32:25 -0400
From: Emily Sours 
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

Content-Type: text/plain; charset=UTF-8

Excellent idea!!

"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks  wrote:

> Hi,
>  I do not disagree at all. in fact, I think it would make for an
> interesting management tool. If you have the nicest CV around, but a simple
> Histonet archive se

Re: [Histonet] Travel Histology Technician Jobs

[Lee & Peggy Wenk]

On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in Providence, 
RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on "Work and Play 
Across the USA - A Guide to Being a Traveling Tech".

http://www.histoconvention.org/
Click on Schedule

Peggy A. Wenk, HTL(ASCP)SLS

-Original Message- 
From: Christopher Jacobs

Sent: Monday, September 09, 2013 1:30 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Travel Histology Technician Jobs

Histonetters,

Does anyone out there have any information on traveling histology technician 
jobs? What is the compensation? How does insurance work? What qualifications 
are needed? I am particularly interested in any personal experiences any one 
has had.


Thanks!

"CJ" Christopher P. Jacobs, HT QIHC(ASCP)
Clin-Path Diagnostics, LLC



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be privileged. If you are not the intended recipient, please contact the 
sender immediately, delete the contents of this message and do not use it 
for any purpose.


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[Histonet] IHC Stainer Repair

[Gwindolyn W Kerce]

I am looking for someone to work on my Dako Autostainer and my Leica ST5050 
stainer in the Atlanta area. Can anyone recommend someone?

Thanks,
Gwin Kerce ASCP(HT) laboratory supervisor
University of Georgia
College of Veterinary Medicine
Dept of Population Health
Poultry Diagnostic and Research Center
Histopathology laboratory rm 244
953 College Station Rd.
Athens, GA 30605
(706) 583-0636
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[Histonet] Re: ICC on previously Wright stained smears

[Teri Johnson]

Hi Susan,

If you are getting background with little cellular staining, then the antigen 
retrieval method isn't working. Rene is correct that the smears you are using 
were most likely methanol fixed.  Although heat induced epitope retrieval is 
thought to be most useful for formalin fixed specimens, there is also some 
benefit to using heat to denature (reconfigure) some of the proteins and you 
may get good staining in minimally fixed or alternatively fixed material. I 
have had success using it in minimally fixed frozen sections, when standard 
dogma says it shouldn't be necessary (no cross-linking occurred).

Your biggest problem may be that the antibody you are using does not recognize 
an alcohol-fixed protein, and no amount of antigen retrieval can fix that.

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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Re: [Histonet] ICC on previously Wright stained smears

[Rene J Buesa]

You need antigen retrieval if you have a specimen that has been fixed with NBF 
but usually blood smears are just fixed with methanol and in this case antigen 
retrieval is not a necessary step.
Eliminate it and you will probably will get better results altogether.
René J.



From: Susan Foreman 
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, September 9, 2013 11:18 AM
Subject: [Histonet] ICC on previously Wright stained smears


Is anyone running ICC on previously Wright-Giemsa stained smears?  What kind
of info can you offer?  We didn't have any staining without antigen
retrieval, so we added that step.  So with the antigen retrieval,  the cells
are in good shape, but now there is too much background without very much
cellular staining.  What recommendations do you have?  Longer or shorter
time in antigen retrieval?  Adjust the pH of the antigen retrieval?  We
don't have very many smears left on this research patient.



Many Thanks,

Susan





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Re: [Histonet] Unsubscribe, Chapter 195

[dusko trajkovic]

Peggy,
I don't think anyone else could have said it better.
Thank you
Dusko



From: Lee & Peggy Wenk 
To: "Manfre, Philip" ; nmhi...@comcast.net; HISTONET 
 
Sent: Monday, September 9, 2013 7:47 AM
Subject: Re: [Histonet] Unsubscribe, Chapter 195


I'm going to wade in, not as someone who has posted numerous times on how to 
unsubscribe, but as someone assessing it from a "risk assessment" evaluation.

If there is a lab task that is consistently being done wrong, by many different 
people, it is usually NOT the fault of the people. It is either a training 
issue, or a process problem. So we either have to do a better job training and 
re-training, or we need to change how the process/procedure is being done.

With the Histonet email, since people are constantly joining, often for a day 
or two, we can't really improve the "training" aspect. Yes, there are 
instructions when we first join, to print off/save how to subscribe or 
unsubscribe or change personal information, etc. But (be honest) how many of us 
pay attention to these types of instructions when we sign up to be a "member" 
of a credit card or a on-line department store or an on-line book store or 
other email lists? Most people do not. So we know that this type of "training" 
is not effective. But we really can't do a one-on-one type of training session 
for each person who signs on to Histonet. Therefore, improving the training is 
not the answer.

The answer lies in modifying the process. Look at the bottom of those emails 
from credit cards or hotels or department stores that you have signed up with. 
There is usually a line that says "If you no longer wish to receive these 
emails, click on this link and follow the instructions".

Add to that, various email lists have various methods on how to unsubscribe, 
which can involve a link, or putting the word unsubscribe in the subject, or 
putting the word unsubscribe in the message.

Histonet has a link at the bottom, but no instructions. So it's not clear to 
click on the link to unsubscribe, nor is there any mention whether one of the 
other unsubscribing methods would work. I therefore believe the Histonet 
unsubscribing procedure has a process problem, that could be easily fixed.

As for the fact that how to unsubscribe has been explained 5,391+ times in the 
past does not help the person who signed up over the weekend, and as of today, 
decided that Histonet is not what they need. This new person has not seen the 
previous requests for help with unsubscribing, nor the answers on how to do it. 
Again, this is a process problem.

Is there any way Histonet can get some clearer instructions at the bottom of 
each email, on how to unsubscribe, either permanently or temporarily while on 
vacation? Such as saying "To unsubscribe, click on the link below, and follow 
the instructions at the bottom of the next webpage".

Let's not yell at the people trying to unsubscribe. Let's work on improving the 
unsubscribing process, so we don't get these requests.

Peggy A. Wenk, HTL(ASCP)SLS

-Original Message- From: Manfre, Philip
Sent: Friday, September 06, 2013 7:30 AM
To: nmhi...@comcast.net ; HISTONET
Subject: RE: [Histonet] Unsubscribe, Chapter 195

Trained professionals should know by now that if you want to unsubscribe, you 
must type in all caps - UNSUBSCRIBE


Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_man...@merck.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
nmhi...@comcast.net
Sent: Thursday, September 05, 2013 9:57 PM
To: HISTONET
Subject: [Histonet] Unsubscribe, Chapter 195

It is a concern that members of our  technically-oriented career field  have a 
difficult time understanding the method for unsubscribing to Histonet. There is 
an  almost- daily posting to "unsubscribe", despite the fact that this subject 
has been addressed literally hundreds of times.  When one "joins" Histonet, 
instructions are provided, should be printed out for reference and used if the 
subscriber decides to leave the group.  We are required to be knowledgeable on 
all manner of technical routines requiring detailed instructions and  Histonet 
is no less clear in the methods for joining and "un-joining".  Use them, 
please.  Fire away - I'm retired and I can take the flak!  I do miss my 
microtome, though...
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http://www.merck.com/contact/contacts.html) t

[Histonet] ICC on previously Wright stained smears

[Susan Foreman]

Is anyone running ICC on previously Wright-Giemsa stained smears?  What kind
of info can you offer?   We didn't have any staining without antigen
retrieval, so we added that step.  So with the antigen retrieval,  the cells
are in good shape, but now there is too much background without very much
cellular staining.  What recommendations do you have?  Longer or shorter
time in antigen retrieval?  Adjust the pH of the antigen retrieval?  We
don't have very many smears left on this research patient.

 

Many Thanks,

Susan

 

 

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Re: [Histonet] Unsubscribe, Chapter 195

[Lee & Peggy Wenk]

I'm going to wade in, not as someone who has posted numerous times on how to 
unsubscribe, but as someone assessing it from a "risk assessment" 
evaluation.


If there is a lab task that is consistently being done wrong, by many 
different people, it is usually NOT the fault of the people. It is either a 
training issue, or a process problem. So we either have to do a better job 
training and re-training, or we need to change how the process/procedure is 
being done.


With the Histonet email, since people are constantly joining, often for a 
day or two, we can't really improve the "training" aspect. Yes, there are 
instructions when we first join, to print off/save how to subscribe or 
unsubscribe or change personal information, etc. But (be honest) how many of 
us pay attention to these types of instructions when we sign up to be a 
"member" of a credit card or a on-line department store or an on-line book 
store or other email lists? Most people do not. So we know that this type of 
"training" is not effective. But we really can't do a one-on-one type of 
training session for each person who signs on to Histonet. Therefore, 
improving the training is not the answer.


The answer lies in modifying the process. Look at the bottom of those emails 
from credit cards or hotels or department stores that you have signed up 
with. There is usually a line that says "If you no longer wish to receive 
these emails, click on this link and follow the instructions".


Add to that, various email lists have various methods on how to unsubscribe, 
which can involve a link, or putting the word unsubscribe in the subject, or 
putting the word unsubscribe in the message.


Histonet has a link at the bottom, but no instructions. So it's not clear to 
click on the link to unsubscribe, nor is there any mention whether one of 
the other unsubscribing methods would work. I therefore believe the Histonet 
unsubscribing procedure has a process problem, that could be easily fixed.


As for the fact that how to unsubscribe has been explained 5,391+ times in 
the past does not help the person who signed up over the weekend, and as of 
today, decided that Histonet is not what they need. This new person has not 
seen the previous requests for help with unsubscribing, nor the answers on 
how to do it. Again, this is a process problem.


Is there any way Histonet can get some clearer instructions at the bottom of 
each email, on how to unsubscribe, either permanently or temporarily while 
on vacation? Such as saying "To unsubscribe, click on the link below, and 
follow the instructions at the bottom of the next webpage".


Let's not yell at the people trying to unsubscribe. Let's work on improving 
the unsubscribing process, so we don't get these requests.


Peggy A. Wenk, HTL(ASCP)SLS

-Original Message- 
From: Manfre, Philip

Sent: Friday, September 06, 2013 7:30 AM
To: nmhi...@comcast.net ; HISTONET
Subject: RE: [Histonet] Unsubscribe, Chapter 195

Trained professionals should know by now that if you want to unsubscribe, 
you must type in all caps - UNSUBSCRIBE



Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_man...@merck.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
nmhi...@comcast.net

Sent: Thursday, September 05, 2013 9:57 PM
To: HISTONET
Subject: [Histonet] Unsubscribe, Chapter 195

It is a concern that members of our  technically-oriented career field  have 
a difficult time understanding the method for unsubscribing to Histonet. 
There is an  almost- daily posting to "unsubscribe", despite the fact that 
this subject has been addressed literally hundreds of times.  When one 
"joins" Histonet, instructions are provided, should be printed out for 
reference and used if the subscriber decides to leave the group.  We are 
required to be knowledgeable on all manner of technical routines requiring 
detailed instructions and  Histonet is no less clear in the methods for 
joining and "un-joining".  Use them, please.  Fire away - I'm retired and I 
can take the flak!  I do miss my microtome, though...

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Re: [Histonet] Unsubscribe, Chapter 195

[nmhisto]

I was happy to read your thoughtful evaluation of the problem and, after having 
read through it, wonder why I hadn't thought of it before!  Perhaps a little 
"tweaking" by the listserv management would make this process much more clear.  
The sign-on page of information about Histonet is rather lengthy (rightly so) 
but perhaps it's time for a little updating.  Thank you, Peggy, for your 
thoughts and proposed solution. 

- Original Message -
From: "Lee & Peggy Wenk"  
To: "Philip Manfre" , nmhi...@comcast.net, "HISTONET" 
 
Sent: Monday, September 9, 2013 8:47:55 AM 
Subject: Re: [Histonet] Unsubscribe, Chapter 195 

I'm going to wade in, not as someone who has posted numerous times on how to 
unsubscribe, but as someone assessing it from a "risk assessment" 
evaluation. 

If there is a lab task that is consistently being done wrong, by many 
different people, it is usually NOT the fault of the people. It is either a 
training issue, or a process problem. So we either have to do a better job 
training and re-training, or we need to change how the process/procedure is 
being done. 

With the Histonet email, since people are constantly joining, often for a 
day or two, we can't really improve the "training" aspect. Yes, there are 
instructions when we first join, to print off/save how to subscribe or 
unsubscribe or change personal information, etc. But (be honest) how many of 
us pay attention to these types of instructions when we sign up to be a 
"member" of a credit card or a on-line department store or an on-line book 
store or other email lists? Most people do not. So we know that this type of 
"training" is not effective. But we really can't do a one-on-one type of 
training session for each person who signs on to Histonet. Therefore, 
improving the training is not the answer. 

The answer lies in modifying the process. Look at the bottom of those emails 
from credit cards or hotels or department stores that you have signed up 
with. There is usually a line that says "If you no longer wish to receive 
these emails, click on this link and follow the instructions". 

Add to that, various email lists have various methods on how to unsubscribe, 
which can involve a link, or putting the word unsubscribe in the subject, or 
putting the word unsubscribe in the message. 

Histonet has a link at the bottom, but no instructions. So it's not clear to 
click on the link to unsubscribe, nor is there any mention whether one of 
the other unsubscribing methods would work. I therefore believe the Histonet 
unsubscribing procedure has a process problem, that could be easily fixed. 

As for the fact that how to unsubscribe has been explained 5,391+ times in 
the past does not help the person who signed up over the weekend, and as of 
today, decided that Histonet is not what they need. This new person has not 
seen the previous requests for help with unsubscribing, nor the answers on 
how to do it. Again, this is a process problem. 

Is there any way Histonet can get some clearer instructions at the bottom of 
each email, on how to unsubscribe, either permanently or temporarily while 
on vacation? Such as saying "To unsubscribe, click on the link below, and 
follow the instructions at the bottom of the next webpage". 

Let's not yell at the people trying to unsubscribe. Let's work on improving 
the unsubscribing process, so we don't get these requests. 

Peggy A. Wenk, HTL(ASCP)SLS 

-Original Message- 
From: Manfre, Philip 
Sent: Friday, September 06, 2013 7:30 AM 
To: nmhi...@comcast.net ; HISTONET 
Subject: RE: [Histonet] Unsubscribe, Chapter 195 

Trained professionals should know by now that if you want to unsubscribe, 
you must type in all caps - UNSUBSCRIBE 


Philip Manfre, B.A., HT (ASCP) 
Associate Principal Scientist 
Merck Research Laboratories 
WP45-251 
PO Box 4 
West Point, PA 19486 

215-652-9750 
215-993-0383 (fax) 
philip_man...@merck.com 




-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
nmhi...@comcast.net 
Sent: Thursday, September 05, 2013 9:57 PM 
To: HISTONET 
Subject: [Histonet] Unsubscribe, Chapter 195 

It is a concern that members of our  technically-oriented career field  have 
a difficult time understanding the method for unsubscribing to Histonet. 
There is an  almost- daily posting to "unsubscribe", despite the fact that 
this subject has been addressed literally hundreds of times.  When one 
"joins" Histonet, instructions are provided, should be printed out for 
reference and used if the subscriber decides to leave the group.  We are 
required to be knowledgeable on all manner of technical routines requiring 
detailed instructions and  Histonet is no less clear in the methods for 
joining and "un-joining".  Use them, please.  Fire away - I'm retired and I 
can take the flak!  I do miss my microtome, though... 
___ 
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[Histonet] RELIA Hot Histology Job Alert Histology Supervisor needed in North Florida.

[Pam Barker]

Hi Histonetters!!
I hope you all had a great weekend!! And I hope your teams NCAA and NFL did
better than mine did!!  I wanted to take a minute and tell you about a
position I recently was engaged on that I am very excited about.
RELIA has been engaged to recruit for a histology supervisor for a private
pathology lab located in North Florida.  This is a busy full staffed private
full service pathology lab which processes all types of tissue and runs a
full menu of special stains and IHC.  The ideal candidate for my client will
have at least 5 years of supervisory experience in a histology lab. HT/HTL
and Florida Supervisor license or elig are required.  My client offers an
excellent salary, benefits and relocation assistance.  For more information
please contact me - Pam Barker toll free at 866-607-3542 until 5pm EST or
anytime on my cell phone at 407-353-5070 or via email at
rel...@earthlink.net to set up a time to talk.  

If you know of someone who might be interested remember RELIA offers a
referral bonus.  If we place someone you refer you will earn a referral
bonus.  
Have a great Monday!!!

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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RE: [Histonet] (no subject)

[Marcum, Pamela A]

The last answer will cover most paraffins used routinely and may cover 
microwave also.  Temperatures below 58C are often melt points however; they may 
not be a complete liquid or have slightly increased viscosity.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 8:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying 
for her boards:



"I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point 
of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have 
also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing 
something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which 
it is addressed and may contain confidential and/or priviledged material.  Any 
review, retransmission, dissemination or other use of, or taking of any action 
in reliance upon, this information by persons or entities other than the 
intended recipient is prohibited.  If you receive this in error, please contact 
the sender and delete the material from any computer.
___
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--
Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of the original 
message.

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RE: [Histonet] (no subject)

[Manfre, Philip]

It's a bad question.  Generally the "best" temperature is probably between 55 
and 65, depending on the paraffin.  This would fall between the two latter 
answers.  Hmm  What to do?

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_man...@merck.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 9:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying 
for her boards:



"I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point 
of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have 
also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing 
something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which 
it is addressed and may contain confidential and/or priviledged material.  Any 
review, retransmission, dissemination or other use of, or taking of any action 
in reliance upon, this information by persons or entities other than the 
intended recipient is prohibited.  If you receive this in error, please contact 
the sender and delete the material from any computer.
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Notice:  This e-mail message, together with any attachments, contains
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please notify us immediately by reply e-mail and then delete it from 
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[Histonet] (no subject)

[Martin, Erin]

Good morning all!

One of our fellows emailed me a question that she came across while studying 
for her boards:



"I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point 
of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have 
also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing 
something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which 
it is addressed and may contain confidential and/or priviledged material.  Any 
review, retransmission, dissemination or other use of, or taking of any action 
in reliance upon, this information by persons or entities other than the 
intended recipient is prohibited.  If you receive this in error, please contact 
the sender and delete the material from any computer.
___
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Re: [Histonet] Unsubscribe, Chapter 195

[Emily Sours]

Excellent idea!!

"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks  wrote:

> Hi,
>  I do not disagree at all. in fact, I think it would make for an
> interesting management tool. If you have the nicest CV around, but a simple
> Histonet archive search for your name + "unsubscribe" shows that you can't
> follow simple instructions, it says something about your communication
> skills and raises interesting questions about weather you are a good
> employment candidate. One might also look for the various incarnations of
> misspelled attempts at unsubscribing such as "unscribe" and "unsiscribe".
>
> Just sayin'
> Amos
>
> On Fri, Sep 6, 2013 at 1:00 PM,
> wrote:
>
> > Message: 6
> > Date: Fri, 6 Sep 2013 01:56:37 + (UTC)
> > From: nmhi...@comcast.net
> > Subject: [Histonet] Unsubscribe, Chapter 195
> > To: HISTONET  
> > Message-ID:
> > <
> >
> 1200426006.1029812.1378432597832.javamail.r...@sz0075a.emeryville.ca.mail.comcast.net
> > >
> >
> > Content-Type: text/plain; charset=utf-8
> >
> > It is a concern that members of our  technically-oriented career field
> > Â have a difficult time understanding the method for unsubscribing to
> > Histonet.  There is an  almost- daily posting to "unsubscribe", despite
> > the fact that this subject has been addressed literally hundreds of
> > times.  When one "joins" Histonet, instructions are provided, should be
> > printed out for reference and used if the subscriber decides to leave
> the
> > group.  We are required to be knowledgeable on all manner of technical
> > routines requiring detailed instructions and  Histonet is no less clear
> in
> > the methods for joining and "un-joining".  Use them, please.  Fire
> away -
> > I'm retired and I can take the flak!  I do miss my microtome, though...
> >
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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