RE: [Histonet] Faxitron Xray digital imaging

2013-11-06 Thread pruegg
Years ago/maybe more than 25 we used a faxitron xray machine to measure bone
calcification on research samples and samples being decaled, it was the best
thing since sliced bread, had to go to the radiology dept to use it and was
told they were dying off the market, loved it, so easy to use and so useful.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com 
rueggihcconsultin...@outlook.com



 

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Tuesday, November 05, 2013 9:47 AM
To: Scott, Allison D; histonet
Subject: RE: [Histonet] Faxitron Xray digital imaging

We have used the Faxitron instrument for the past 3 years at multiple sites.
Faxitron creates a digital image of a specimen quickly and safely and a t
multiple magnifications. The instrument is placed in either Surgery or
Surgical Pathology (SP) and we use it primarily on breast cases to assist in
orientation and gross dissection, identifying radioactive seeds or locating
micro calcification. The instrument is easy to use, safe and you can connect
to multiple LIS systems and a Hospital PACS. There is no code to charge for
use when used in SP, only when used by Radiology or Surgery.
 
There is only one company that manufactures and sells the instrument:
Faxitron Bioptics, LLC
Tucson, AZ
877-910-0030
 
The are willing to bring a unit to your site for demonstration and you may
be able to talk them into leaving for a evaluation.
William DeSalvo, BS HTL(ASCP)

 
> From: allison.sc...@harrishealth.org
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 5 Nov 2013 16:00:11 +
> Subject: [Histonet] Faxitron Xray digital imaging
> 
> Hello to all in histoland.  Our chief pathologist is interested in getting
a faxitron xray digital imaging machine.  Is anyone out there using this
technology and can you suggest a company in which to order one from.  Any
help in this will be greatly appreciated.
> 
> 
> 
> 
> Allison Scott HT(ASCP)
> Supervisor, Histology Lab
> LBJ Hospital
> Harris Health System
> Office: 713-566-2148
> Lab: 713-566-5287
> 
> 
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RE: [Histonet] CD 34 antibody abcam (ab963)

2013-11-06 Thread pruegg
This is a tuff one CD34 will stain all precursor cells not just endothelial 
cells for vessels, there is an excellent CD31 for ms tissue from Dionova it is 
rat anti ms but not sure of what you could use for cd31 for rat and rabbits, 
but I know cd34 is not the way to go.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com 
rueggihcconsultin...@outlook.com



 

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dissemination, distribution, copying, or other use of this message, or any of 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahseen
Sent: Wednesday, November 06, 2013 5:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD 34 antibody abcam (ab963)

Hi All,
We used CD 34 antibody abcam (ab963)with detection kit enVision to stain our 
samples from rats and rabbits. We need to stain vessels only but macrophages, 
fibroblast, collagen all are stained in other hand known +ve control (Tonsil) 
is perform good.  Please help us that what should we do for IHC staining of our 
samples.
Best regards
Muhammad Tahseen
Sr.Supervisor Histology
SKMCH&RC
Lahore


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RE: [Histonet] FDA Disclaimer

2013-11-06 Thread pruegg
I would think so, 

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com 
rueggihcconsultin...@outlook.com



 

This email is confidential and intended solely for the use of the Person(s)
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presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
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this e-mail as soon as possible.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen,
Valerie
Sent: Tuesday, November 05, 2013 3:48 AM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] FDA Disclaimer

Good Morning...

I have a question about the FDA disclaimer for Immuno's.  If we are not
doing the staining of Immuno's in our lab, but our Pathologists are
interpreting those that are stained at our reference lab, are we still
required to put the FDA disclaimer on our Path reports for those antibodies
that require the disclaimer?

Thanks so much!!


Valerie

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com


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RE: [Histonet] On call hours required of staff

2013-11-06 Thread joelle weaver
Hazel
Yes,  that sounds more reasonable. I have been on call for many years while on 
the bench- just never that much, or for that many consecutive hours 
individually. You always have set hours that you have to be available and 
respond within certain time frames and the days are rotated for on call. I 
never minded that much, and you can usually trade with someone if you have 
something important you need to do that takes you out of town.  Of course, I 
would hope for some compensation for the obliteration of my personal life with 
that many hours.  Appreciate your thoughts on this. 
If your lab has a policy for this that you can attach or copy to me the 
highlights in an email this will help me bargain.




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: hor...@archildrens.org
> To: joellewea...@hotmail.com; mpe...@grhs.net; 
> histonet@lists.utsouthwestern.edu
> Date: Wed, 6 Nov 2013 15:01:29 -0600
> Subject: RE: [Histonet] On call hours required of staff
> 
> I would not ask my techs to take call for no pay.We have to respond to 
> call in 1 hour.  The tech would not be able to make personal plans if they 
> are on call.   
> 
> We are on call on long weekends.  We take turns taking call and are paid call 
> pay.  If we get called in, it is overtime.   On the rare occasion someone 
> needs to work Saturday, one of us volunteer and are paid call pay and 
> overtime.
> 
> As a supervisor I am potentially on call all of the time.   If our tissue 
> processor alarms, I am called.  I come in and I get call pay and overtime. 
> (This is a rare occurrence)   If I am unavailable, my senior tech answers 
> calls.  I also answer after hours calls from the lab.  Usually a phone call 
> takes care of it.
> 
> Hazel Horn
> Supervisor of Histology/Autopsy/Transcription
> Anatomic Pathology
> Arkansas Children's Hospital
> 1 Children's Way | Slot 820| Little Rock, AR 72202
> 501.364.4240 direct | 501.364.1241 fax
> hor...@archildrens.org
> archildrens.org
> 
> 
> 
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: Wednesday, November 06, 2013 2:46 PM
> To: Mike Pence; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On call hours required of staff
> 
> No pay has been mentioned. The call hours are to be covered initially by one 
> person .
>  
> The tasks are potentially take care of anything occurring during those hours 
> including stat specimens. The primary concern of management is that a 
> refrigerator or the room temperature or humidity will go out of range when 
> staff are not on site.  There is a 24 hour monitor for environmental stuff 
> that will call to the person's cell phone, and anything else that occurs when 
> no one is scheduled in the area will result in a cell call. 
> 
> 
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>  
> > From: mpe...@grhs.net
> > To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu
> > Subject: RE: [Histonet] On call hours required of staff
> > Date: Wed, 6 Nov 2013 20:34:17 +
> > 
> > Will the techs be paid call pay? What is being asked of the techs to do on 
> > call?
> > 
> > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu 
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
> > weaver
> > Sent: Wednesday, November 06, 2013 2:26 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] On call hours required of staff
> > 
> >  Sorry if this re-posted- it got bounced back to me once.
> >  
> > Please advise of the number of hours that your histology staff are 
> > scheduled to take on-call in addition to their 40 scheduled hours ( FT 
> > staff/hourly) ? 
> > If you have a maximum # of on call hours by your lab or HR policies, that 
> > would be most helpful.
> > The proposal is to begin a usual 40 worked hours scheduled in the lab and 
> > then ~ 15 hours overnight on call for each weekday,( M-F),  and potentially 
> > 8 hours or more each weekend day. I think this proposal is unreasonable, 
> > and 75 hours + 16 hours potential on call/week ( 91 hours on call?). 
> > I would appreciate your thoughts , comments? I hope to derail this with 
> > something more reasonable
> > 
> > 
> > Joelle Weaver MAOM, HTL (ASCP) QIHC
> >   
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> > 
> 
> ___
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> 
> **

RE: [Histonet] On call hours required of staff

2013-11-06 Thread joelle weaver
Is that an employment law you quote? There is only one person, and they are not 
able to work that many hours at this point in their life, or even willing to do 
so for any amount of money.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mpe...@grhs.net
> To: hor...@archildrens.org; joellewea...@hotmail.com; 
> histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On call hours required of staff
> Date: Wed, 6 Nov 2013 21:08:29 +
> 
> No pay, no call! I don't think you could require your techs to take call 
> without paying them for their on-call time. Maybe you have one or two techs 
> that would not mind the extra money being on call. I remember back several 
> years ago (maybe a million) when I was younger I would have taken anything 
> for more money!
> 
> -Original Message-
> From: Horn, Hazel V [mailto:hor...@archildrens.org] 
> Sent: Wednesday, November 06, 2013 3:01 PM
> To: 'joelle weaver'; Mike Pence; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On call hours required of staff
> 
> I would not ask my techs to take call for no pay.We have to respond to 
> call in 1 hour.  The tech would not be able to make personal plans if they 
> are on call.   
> 
> We are on call on long weekends.  We take turns taking call and are paid call 
> pay.  If we get called in, it is overtime.   On the rare occasion someone 
> needs to work Saturday, one of us volunteer and are paid call pay and 
> overtime.
> 
> As a supervisor I am potentially on call all of the time.   If our tissue 
> processor alarms, I am called.  I come in and I get call pay and overtime. 
> (This is a rare occurrence)   If I am unavailable, my senior tech answers 
> calls.  I also answer after hours calls from the lab.  Usually a phone call 
> takes care of it.
> 
> Hazel Horn
> Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
> Children's Hospital
> 1 Children's Way | Slot 820| Little Rock, AR 72202
> 501.364.4240 direct | 501.364.1241 fax
> hor...@archildrens.org
> archildrens.org
> 
> 
> 
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: Wednesday, November 06, 2013 2:46 PM
> To: Mike Pence; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On call hours required of staff
> 
> No pay has been mentioned. The call hours are to be covered initially by one 
> person .
>  
> The tasks are potentially take care of anything occurring during those hours 
> including stat specimens. The primary concern of management is that a 
> refrigerator or the room temperature or humidity will go out of range when 
> staff are not on site.  There is a 24 hour monitor for environmental stuff 
> that will call to the person's cell phone, and anything else that occurs when 
> no one is scheduled in the area will result in a cell call. 
> 
> 
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>  
> > From: mpe...@grhs.net
> > To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu
> > Subject: RE: [Histonet] On call hours required of staff
> > Date: Wed, 6 Nov 2013 20:34:17 +
> > 
> > Will the techs be paid call pay? What is being asked of the techs to do on 
> > call?
> > 
> > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu 
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
> > weaver
> > Sent: Wednesday, November 06, 2013 2:26 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] On call hours required of staff
> > 
> >  Sorry if this re-posted- it got bounced back to me once.
> >  
> > Please advise of the number of hours that your histology staff are 
> > scheduled to take on-call in addition to their 40 scheduled hours ( FT 
> > staff/hourly) ? 
> > If you have a maximum # of on call hours by your lab or HR policies, that 
> > would be most helpful.
> > The proposal is to begin a usual 40 worked hours scheduled in the lab and 
> > then ~ 15 hours overnight on call for each weekday,( M-F),  and potentially 
> > 8 hours or more each weekend day. I think this proposal is unreasonable, 
> > and 75 hours + 16 hours potential on call/week ( 91 hours on call?). 
> > I would appreciate your thoughts , comments? I hope to derail this 
> > with something more reasonable
> > 
> > 
> > Joelle Weaver MAOM, HTL (ASCP) QIHC
> >   
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> > 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 

RE: [Histonet] On call hours required of staff

2013-11-06 Thread Mike Pence
No pay, no call! I don't think you could require your techs to take call 
without paying them for their on-call time. Maybe you have one or two techs 
that would not mind the extra money being on call. I remember back several 
years ago (maybe a million) when I was younger I would have taken anything for 
more money!

-Original Message-
From: Horn, Hazel V [mailto:hor...@archildrens.org] 
Sent: Wednesday, November 06, 2013 3:01 PM
To: 'joelle weaver'; Mike Pence; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On call hours required of staff

I would not ask my techs to take call for no pay.We have to respond to call 
in 1 hour.  The tech would not be able to make personal plans if they are on 
call.   

We are on call on long weekends.  We take turns taking call and are paid call 
pay.  If we get called in, it is overtime.   On the rare occasion someone needs 
to work Saturday, one of us volunteer and are paid call pay and overtime.

As a supervisor I am potentially on call all of the time.   If our tissue 
processor alarms, I am called.  I come in and I get call pay and overtime. 
(This is a rare occurrence)   If I am unavailable, my senior tech answers 
calls.  I also answer after hours calls from the lab.  Usually a phone call 
takes care of it.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, November 06, 2013 2:46 PM
To: Mike Pence; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On call hours required of staff

No pay has been mentioned. The call hours are to be covered initially by one 
person .
 
The tasks are potentially take care of anything occurring during those hours 
including stat specimens. The primary concern of management is that a 
refrigerator or the room temperature or humidity will go out of range when 
staff are not on site.  There is a 24 hour monitor for environmental stuff that 
will call to the person's cell phone, and anything else that occurs when no one 
is scheduled in the area will result in a cell call. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mpe...@grhs.net
> To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On call hours required of staff
> Date: Wed, 6 Nov 2013 20:34:17 +
> 
> Will the techs be paid call pay? What is being asked of the techs to do on 
> call?
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
> weaver
> Sent: Wednesday, November 06, 2013 2:26 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] On call hours required of staff
> 
>  Sorry if this re-posted- it got bounced back to me once.
>  
> Please advise of the number of hours that your histology staff are scheduled 
> to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? 
> If you have a maximum # of on call hours by your lab or HR policies, that 
> would be most helpful.
> The proposal is to begin a usual 40 worked hours scheduled in the lab and 
> then ~ 15 hours overnight on call for each weekday,( M-F),  and potentially 8 
> hours or more each weekend day. I think this proposal is unreasonable, and 75 
> hours + 16 hours potential on call/week ( 91 hours on call?). 
> I would appreciate your thoughts , comments? I hope to derail this 
> with something more reasonable
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
  
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RE: [Histonet] On call hours required of staff

2013-11-06 Thread Horn, Hazel V
I would not ask my techs to take call for no pay.We have to respond to call 
in 1 hour.  The tech would not be able to make personal plans if they are on 
call.   

We are on call on long weekends.  We take turns taking call and are paid call 
pay.  If we get called in, it is overtime.   On the rare occasion someone needs 
to work Saturday, one of us volunteer and are paid call pay and overtime.

As a supervisor I am potentially on call all of the time.   If our tissue 
processor alarms, I am called.  I come in and I get call pay and overtime. 
(This is a rare occurrence)   If I am unavailable, my senior tech answers 
calls.  I also answer after hours calls from the lab.  Usually a phone call 
takes care of it.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, November 06, 2013 2:46 PM
To: Mike Pence; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On call hours required of staff

No pay has been mentioned. The call hours are to be covered initially by one 
person .
 
The tasks are potentially take care of anything occurring during those hours 
including stat specimens. The primary concern of management is that a 
refrigerator or the room temperature or humidity will go out of range when 
staff are not on site.  There is a 24 hour monitor for environmental stuff that 
will call to the person's cell phone, and anything else that occurs when no one 
is scheduled in the area will result in a cell call. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mpe...@grhs.net
> To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On call hours required of staff
> Date: Wed, 6 Nov 2013 20:34:17 +
> 
> Will the techs be paid call pay? What is being asked of the techs to do on 
> call?
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: Wednesday, November 06, 2013 2:26 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] On call hours required of staff
> 
>  Sorry if this re-posted- it got bounced back to me once.
>  
> Please advise of the number of hours that your histology staff are scheduled 
> to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? 
> If you have a maximum # of on call hours by your lab or HR policies, that 
> would be most helpful.
> The proposal is to begin a usual 40 worked hours scheduled in the lab and 
> then ~ 15 hours overnight on call for each weekday,( M-F),  and potentially 8 
> hours or more each weekend day. I think this proposal is unreasonable, and 75 
> hours + 16 hours potential on call/week ( 91 hours on call?). 
> I would appreciate your thoughts , comments? I hope to derail this with 
> something more reasonable
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
  
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and protected from disclosure. If the reader of this message is not the 
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message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

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RE: [Histonet] On call hours required of staff

2013-11-06 Thread joelle weaver
No pay has been mentioned. The call hours are to be covered initially by one 
person .
 
The tasks are potentially take care of anything occurring during those hours 
including stat specimens. The primary concern of management is that a 
refrigerator or the room temperature or humidity will go out of range when 
staff are not on site.  There is a 24 hour monitor for environmental stuff that 
will call to the person's cell phone, and anything else that occurs when no one 
is scheduled in the area will result in a cell call. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mpe...@grhs.net
> To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On call hours required of staff
> Date: Wed, 6 Nov 2013 20:34:17 +
> 
> Will the techs be paid call pay? What is being asked of the techs to do on 
> call?
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: Wednesday, November 06, 2013 2:26 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] On call hours required of staff
> 
>  Sorry if this re-posted- it got bounced back to me once.
>  
> Please advise of the number of hours that your histology staff are scheduled 
> to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? 
> If you have a maximum # of on call hours by your lab or HR policies, that 
> would be most helpful.
> The proposal is to begin a usual 40 worked hours scheduled in the lab and 
> then ~ 15 hours overnight on call for each weekday,( M-F),  and potentially 8 
> hours or more each weekend day. I think this proposal is unreasonable, and 75 
> hours + 16 hours potential on call/week ( 91 hours on call?). 
> I would appreciate your thoughts , comments? I hope to derail this with 
> something more reasonable
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
> 
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> 
> 
  
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RE: [Histonet] On call hours required of staff

2013-11-06 Thread Mike Pence
Will the techs be paid call pay? What is being asked of the techs to do on call?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, November 06, 2013 2:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On call hours required of staff

 Sorry if this re-posted- it got bounced back to me once.
 
Please advise of the number of hours that your histology staff are scheduled to 
take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? 
If you have a maximum # of on call hours by your lab or HR policies, that would 
be most helpful.
The proposal is to begin a usual 40 worked hours scheduled in the lab and then 
~ 15 hours overnight on call for each weekday,( M-F),  and potentially 8 hours 
or more each weekend day. I think this proposal is unreasonable, and 75 hours + 
16 hours potential on call/week ( 91 hours on call?). 
I would appreciate your thoughts , comments? I hope to derail this with 
something more reasonable


Joelle Weaver MAOM, HTL (ASCP) QIHC
  
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[Histonet] On call hours required of staff

2013-11-06 Thread joelle weaver
 Sorry if this re-posted- it got bounced back to me once.
 
Please advise of the number of hours that your histology staff are scheduled to 
take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? 
If you have a maximum # of on call hours by your lab or HR policies, that would 
be most helpful.
The proposal is to begin a usual 40 worked hours scheduled in the lab and then 
~ 15 hours overnight on call for each weekday,( M-F),  and potentially 8 hours 
or more each weekend day. I think this proposal is unreasonable, and 75 hours + 
16 hours potential on call/week ( 91 hours on call?). 
I would appreciate your thoughts , comments? I hope to derail this with 
something more reasonable


Joelle Weaver MAOM, HTL (ASCP) QIHC
  
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[Histonet] Re: Fungus controls

2013-11-06 Thread Bob Richmond
I wouldn't be too happy with any of these substitutes for proper fungus
controls. Ideally, your control would be the fungus you're looking for,
obviously not a practical way to do it.

The usual fungus control I see is mouse lung with yeast, presumably
Candida, injected into normal lung tissue. This control will stain very
satisfactorily with PAS, and is not really a good test of the efficacy of a
fungus stain.

The most exacting fungus control for the GMS stain is histoplasma, an old
infection in which some of the yeast cells are actually dead. If you can
stain that, you can stain any fungus you're likely to encounter. Freida
Carson did an elegant study a number of years ago (reference below) of how
critical the oxidation step is in this endeavor.

The decline of medical autopsies has made controls like this much more
difficult to obtain, but when you can get it, you can get large quantities
of it which need to be effectively shared.

Bob Richmond
Samurai Pathologist
Maryville TN
***
Inconsistent Detection of Histoplasma capsulatum with Periodic Acid
Oxidation in the Grocott Methenamine-Silver Nitrate (GMS) Fungus Stain

Freida L. Carson, Jerry Fredenburgh, and John E. Maxwell
1. Department of Pathology, Baylor University Medical Center
2. Richard-Allan Scientific Kalamazoo MI
3. Glenwood Regional Medical Center, West Monroe LA

J Histotechnol [June] 1999;22:119
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RE: [Histonet] Biopsy Problems

2013-11-06 Thread McAnn, Sherrian
Seems as though they may  be lifting off in spots? Or maybe perhaps
vibration?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne
Clark
Sent: Wednesday, November 06, 2013 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biopsy Problems


Hi Fellow Histonetters, we have started having this problem with our
G.I. biopsies, and not all of them, just the odd one here and there.
After staining, when looking at it on the scope, you can't get it to
focus in one plane.  It's not cutting artifact, because we have recut
the block making sure it is well cooled etc. before picking up and
staining the section.  The cells, when in focus, look fixed and properly
processed, and the staining is the way it should be.  I have no idea
what is causing this artifact.  Has anyone else seen this and can shed
some light on what is causing it?

Joanne Clark, HT
Director of Histology
Pathology Consultants of New Mexico




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[Histonet] RE: unsubscribe

2013-11-06 Thread Weems, Joyce K.
Don't fuss at her - I told her what to do!! :>)

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McCarty, Jean 
[JRDUS]
Sent: Wednesday, November 06, 2013 1:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] unsubscribe



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, November 06, 2013 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 120, Issue 7

Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. FW: Fungus Controls (Tim Higgins)
   2. RE: Histonet Digest, Vol 120, Issue 6 (Gray, Ed)
   3. RE: FDA Disclaimer (Hannen, Valerie) (Jean Wood)
   4. RE: FW: Fungus Controls (Tom McNemar)
   5. CD 34 antibody abcam (ab963) (tahseen)
   6. Water rinse for H&E's (Pardue, Judith)
   7. Re: Water rinse for H&E's (Rene J Buesa)
   8. RE: Water rinse for H&E's (Terri Brown)
   9. Biopsy Problems (Joanne Clark)


--

Message: 1
Date: Tue, 5 Nov 2013 13:03:54 -0600
From: Tim Higgins 
Subject: [Histonet] FW: Fungus Controls
To: "histonet@lists.utsouthwestern.edu"
,"vickroy@mhsil.com"

Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"




I have used orange peel for years. I never had a problem with the pathologist 
not approving the control for use on patient stains.  Its "naturally 
occurring", a petri dish would not be naturally occurring in my mind.

Once I verified the orange peel does stain the fungus the same way it does in 
human tissue by doing a side by side staining the pathologist were fine with 
it.  Actually happy I was able to save some money.

Most controls you get from other source are not human either, usually it some 
type of animal. Try the orange peel. I did like the idea of using the some type 
of moldy meat or having your micro department do something for you  I have 
never tried that, but if it works that would be awesome.   Thanks,

Timothy N. Higgins, HT (ASCP), QIHC


> Message: 4
> Date: Mon, 4 Nov 2013 15:07:06 -0500
> From: Patrick Laurie 
> Subject: Re: [Histonet] Aspergillus tissue blocks for controls
> To: "Vickroy, Jim" 
> Cc: "histonet@lists.utsouthwestern.edu"
>   
> Message-ID:
>   
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Jim,
>
> I have found a couple of ways.  First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic).  Another method, which doesn't work the best,  can be
> moldy orange peel.  And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
>
> Good luck!
>
>
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim  wrote:
>
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
> >
> >


--

Message: 2
Date: Tue, 5 Nov 2013 19:20:42 +
From: "Gray, Ed" 
Subject: [Histonet] RE: Histonet Digest, Vol 120, Issue 6
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

<6a889df9e216449bbac73ab5d795a...@bn1pr05mb406.namprd05.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

You actually need the performing lab's disclaimer.  We'

[Histonet] unsubscribe

2013-11-06 Thread McCarty, Jean [JRDUS]


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, November 06, 2013 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 120, Issue 7

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. FW: Fungus Controls (Tim Higgins)
   2. RE: Histonet Digest, Vol 120, Issue 6 (Gray, Ed)
   3. RE: FDA Disclaimer (Hannen, Valerie) (Jean Wood)
   4. RE: FW: Fungus Controls (Tom McNemar)
   5. CD 34 antibody abcam (ab963) (tahseen)
   6. Water rinse for H&E's (Pardue, Judith)
   7. Re: Water rinse for H&E's (Rene J Buesa)
   8. RE: Water rinse for H&E's (Terri Brown)
   9. Biopsy Problems (Joanne Clark)


--

Message: 1
Date: Tue, 5 Nov 2013 13:03:54 -0600
From: Tim Higgins 
Subject: [Histonet] FW: Fungus Controls
To: "histonet@lists.utsouthwestern.edu"
,"vickroy@mhsil.com"

Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"




I have used orange peel for years. I never had a problem with the pathologist 
not approving the control for use on patient stains.  Its "naturally 
occurring", a petri dish would not be naturally occurring in my mind.
 
Once I verified the orange peel does stain the fungus the same way it does in 
human tissue by doing a side by side staining the pathologist were fine with 
it.  Actually happy I was able to save some money.
 
Most controls you get from other source are not human either, usually it some 
type of animal. Try the orange peel. I did like the idea of using the some type 
of moldy meat or having your micro department do something for you  I have 
never tried that, but if it works that would be awesome.   Thanks,

Timothy N. Higgins, HT (ASCP), QIHC
 

> Message: 4
> Date: Mon, 4 Nov 2013 15:07:06 -0500
> From: Patrick Laurie 
> Subject: Re: [Histonet] Aspergillus tissue blocks for controls
> To: "Vickroy, Jim" 
> Cc: "histonet@lists.utsouthwestern.edu"
>   
> Message-ID:
>   
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Hi Jim,
> 
> I have found a couple of ways.  First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic).  Another method, which doesn't work the best,  can be
> moldy orange peel.  And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
> 
> Good luck!
> 
> 
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim  wrote:
> 
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
> >
> >
  

--

Message: 2
Date: Tue, 5 Nov 2013 19:20:42 +
From: "Gray, Ed" 
Subject: [Histonet] RE: Histonet Digest, Vol 120, Issue 6
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

<6a889df9e216449bbac73ab5d795a...@bn1pr05mb406.namprd05.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

You actually need the performing lab's disclaimer.  We've sent stains and other 
procedures to several other labs.  We refer to the disclaimers as ASR's 
(analyte specific reagents) and build templates for each lab's specific 
comments in our APLIS.

Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU 
Healthcare l eg...@wvuhealthcare.com

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message.

Message: 9
Date: Tue, 5 Nov 2013 05:47:53 -0500
From: "Hannen, Valerie" 
Subject: [Histonet] FDA Disclaimer
To: "Histonet Post (histonet@lists.utsouthwestern.edu)"
 

[Histonet] Biopsy Problems

2013-11-06 Thread Joanne Clark

Hi Fellow Histonetters, we have started having this problem with our G.I. 
biopsies, and not all of them, just the odd one here and there.  After 
staining, when looking at it on the scope, you can't get it to focus in one 
plane.  It's not cutting artifact, because we have recut the block making sure 
it is well cooled etc. before picking up and staining the section.  The cells, 
when in focus, look fixed and properly processed, and the staining is the way 
it should be.  I have no idea what is causing this artifact.  Has anyone else 
seen this and can shed some light on what is causing it?

Joanne Clark, HT
Director of Histology
Pathology Consultants of New Mexico




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histonet@lists.utsouthwestern.edu

2013-11-06 Thread Terri Brown
We use warm water rinses for our H&E slides on our automatic stainers.

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.br...@northside.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue,
Judith
Sent: Wednesday, November 06, 2013 10:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water rinse for H&E's

Does anyone use hot water rinses on their h&e slides. If we use cold
water our slides are very light and inconsistent.
We stain on an automatic stainer.


Judith Pardue
Histology Supervisor
Memorial Health Care System
judith_par...@memorial.org

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histonet@lists.utsouthwestern.edu

2013-11-06 Thread Rene J Buesa
I do not, but it seems that you need to use hot water. If it works for you, 
keep doing it.
 
The rationale behind this issue is that water after the staining is not just 
waterto wash the sections, but also to change the pH of the hematoxylin so the 
stain is, lets say, "developed" and this is a process temperature driven (the 
speed of the reaction is directly proportional to the temp.) and if you are 
having weak results with your running water (evidently cold) and good with hot 
water, this demonstrates that you need hot water in your specific circumstances.
I hope I explained myself
René J.



From: "Pardue, Judith" 
To: "histonet@lists.utsouthwestern.edu"  
Sent: Wednesday, November 6, 2013 10:38 AM
Subject: [Histonet] Water rinse for H&E's


Does anyone use hot water rinses on their h&e slides. If we use cold water our 
slides are very light and inconsistent.
We stain on an automatic stainer.


Judith Pardue
Histology Supervisor
Memorial Health Care System
judith_par...@memorial.org

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2013-11-06 Thread Pardue, Judith
Does anyone use hot water rinses on their h&e slides. If we use cold water our 
slides are very light and inconsistent.
We stain on an automatic stainer.


Judith Pardue
Histology Supervisor
Memorial Health Care System
judith_par...@memorial.org

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[Histonet] CD 34 antibody abcam (ab963)

2013-11-06 Thread tahseen

Hi All,
We used CD 34 antibody abcam (ab963)with detection kit enVision to 
stain our samples from rats and rabbits. We need to stain vessels only 
but macrophages, fibroblast, collagen all are stained in other hand 
known +ve control (Tonsil) is perform good.  Please help us that what 
should we do for IHC staining of our samples.

Best regards
Muhammad Tahseen
Sr.Supervisor Histology
SKMCH&RC
Lahore


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RE: [Histonet] FW: Fungus Controls

2013-11-06 Thread Tom McNemar
I made some controls years ago that we are still using today.  I used bread 
mold and streaked it out on a blood agar plate.  Once the plate was covered, 
cut the agar into cubes, wrapped it in lens paper and processed.  Cuts and 
stains beautifully.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Tuesday, November 05, 2013 2:04 PM
To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com
Subject: [Histonet] FW: Fungus Controls




I have used orange peel for years. I never had a problem with the pathologist 
not approving the control for use on patient stains.  Its "naturally 
occurring", a petri dish would not be naturally occurring in my mind.

Once I verified the orange peel does stain the fungus the same way it does in 
human tissue by doing a side by side staining the pathologist were fine with 
it.  Actually happy I was able to save some money.

Most controls you get from other source are not human either, usually it some 
type of animal. Try the orange peel. I did like the idea of using the some type 
of moldy meat or having your micro department do something for you  I have 
never tried that, but if it works that would be awesome.   Thanks,

Timothy N. Higgins, HT (ASCP), QIHC


> Message: 4
> Date: Mon, 4 Nov 2013 15:07:06 -0500
> From: Patrick Laurie 
> Subject: Re: [Histonet] Aspergillus tissue blocks for controls
> To: "Vickroy, Jim" 
> Cc: "histonet@lists.utsouthwestern.edu"
>   
> Message-ID:
>   
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Jim,
>
> I have found a couple of ways.  First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic).  Another method, which doesn't work the best,  can be
> moldy orange peel.  And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
>
> Good luck!
>
>
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim  wrote:
>
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
> >
> >
  
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